AlfalfaGEDB | MS.gene000609

M. sativa cultivar XinJiangDaYe / MS.gene000609

KEGG: K12581
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04857.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000609.t1	XP_024638824.1	99.6	277	1	0	1	277	1	277	1.00E-160	575.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000609.t1	Q9SKZ2	79.9	269	54	0	9	277	7	275	3.0e-132	472.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000609.t1	A0A396HYE4	99.6	277	1	0	1	277	1	277	7.4e-161	575.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene000609	MS.gene21541	PPI
MS.gene000609	MS.gene38199	PPI
MS.gene000609	MS.gene85901	PPI
MS.gene35444	MS.gene000609	PPI
MS.gene000609	MS.gene53123	PPI
MS.gene000609	MS.gene78556	PPI
MS.gene000609	MS.gene048793	PPI
MS.gene81219	MS.gene000609	PPI
MS.gene053225	MS.gene000609	PPI
MS.gene034250	MS.gene000609	PPI
MS.gene000609	MS.gene35431	PPI
MS.gene034816	MS.gene000609	PPI
MS.gene53120	MS.gene000609	PPI
MS.gene72978	MS.gene000609	PPI
MS.gene72178	MS.gene000609	PPI
MS.gene034815	MS.gene000609	PPI
MS.gene68798	MS.gene000609	PPI
MS.gene000609	MS.gene007154	PPI
MS.gene000609	MS.gene41101	PPI
MS.gene000609	MS.gene72156	PPI
MS.gene55324	MS.gene000609	PPI
MS.gene000609	MS.gene048794	PPI
MS.gene025629	MS.gene000609	PPI
MS.gene000609	MS.gene014264	PPI
MS.gene000609	MS.gene35692	PPI
MS.gene41100	MS.gene000609	PPI
MS.gene000609	MS.gene53122	PPI
MS.gene000609	MS.gene85905	PPI
MS.gene53121	MS.gene000609	PPI
MS.gene000609	MS.gene065709	PPI
MS.gene000609	MS.gene72977	PPI
MS.gene000609	MS.gene065707	PPI
MS.gene000609	MS.gene025628	PPI
MS.gene030414	MS.gene000609	PPI
MS.gene030415	MS.gene000609	PPI
MS.gene000609	MS.gene85904	PPI
MS.gene000609	MS.gene84309	PPI
MS.gene68205	MS.gene000609	PPI
MS.gene070320	MS.gene000609	PPI
MS.gene78557	MS.gene000609	PPI
MS.gene72160	MS.gene000609	PPI
MS.gene000609	MS.gene034438	PPI
MS.gene000609	MS.gene41097	PPI
MS.gene38057	MS.gene000609	PPI
MS.gene38201	MS.gene000609	PPI
MS.gene000609	MS.gene53119	PPI
MS.gene000609	MS.gene020895	PPI
MS.gene065708	MS.gene000609	PPI
MS.gene000609	MS.gene72157	PPI
MS.gene85903	MS.gene000609	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000609.t1	AT2G32070	79.926	269	54	0	9	277	7	275	4.73e-169	469
MS.gene000609.t1	AT1G80780	79.323	266	54	1	9	274	7	271	2.46e-162	452
MS.gene000609.t1	AT1G80780	79.323	266	54	1	9	274	7	271	2.46e-162	452
MS.gene000609.t1	AT1G80780	79.167	264	54	1	9	272	7	269	1.87e-160	447
MS.gene000609.t1	AT5G10960	72.119	269	74	1	8	276	6	273	1.44e-146	412
MS.gene000609.t1	AT1G15920	63.433	268	91	2	7	268	9	275	1.78e-122	351
MS.gene000609.t1	AT1G15920	63.433	268	91	2	7	268	9	275	1.78e-122	351
MS.gene000609.t1	AT3G44260	53.571	280	119	8	1	270	1	279	1.25e-94	280
MS.gene000609.t1	AT5G22250	53.731	268	117	6	10	270	10	277	1.56e-94	280
MS.gene000609.t1	AT1G06450	37.405	262	147	6	15	272	10	258	1.56e-47	162
MS.gene000609.t1	AT1G61470	35.000	240	135	7	16	249	4	228	3.69e-34	125
MS.gene000609.t1	AT3G44240	34.934	229	136	5	27	253	2	219	4.69e-33	121
MS.gene000609.t1	AT1G27820	34.156	243	135	9	16	249	8	234	4.76e-31	117
MS.gene000609.t1	AT1G27890	34.568	243	134	9	16	249	3	229	1.59e-29	113

Find 45 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCATAGTGGATATGATTT+TGG	0.178563	4.3:+88603511	MS.gene000609:CDS
CGATTCAGATCCGTGAAGTT+TGG	0.267035	4.3:+88603053	MS.gene000609:CDS
TACTCACATCTTCTGCATTC+AGG	0.268390	4.3:+88603740	MS.gene000609:CDS
GAATTTCTTTAGTGGATCTT+TGG	0.269835	4.3:+88603774	MS.gene000609:CDS
TATGTTAAAGCTTATACAAT+TGG	0.337330	4.3:+88603237	MS.gene000609:CDS
AAGAACCCAACTTGTGTATC+AGG	0.337532	4.3:-88603568	None:intergenic
CAACTTGCGGCGAAGATGAT+AGG	0.351788	4.3:+88603293	MS.gene000609:CDS
TTGAAAGAGAATTTCTTTAG+TGG	0.358856	4.3:+88603766	MS.gene000609:CDS
GGTATCGATGCTAGGCGTTT+CGG	0.383381	4.3:+88603433	MS.gene000609:CDS
TGTTGGAGGTTAAGAGGGTT+GGG	0.387020	4.3:+88603689	MS.gene000609:CDS
CAGAGTTGTTGGAGGTTAAG+AGG	0.391647	4.3:+88603683	MS.gene000609:CDS
TCACAGCGAATTCTTCTTCA+AGG	0.398899	4.3:-88603084	None:intergenic
AAATATGCAGGGGTCTTGTA+TGG	0.400647	4.3:+88603799	MS.gene000609:CDS
TGATTATCCTTTCATTGCTA+TGG	0.408589	4.3:+88603123	MS.gene000609:CDS
TTGTTGGAGGTTAAGAGGGT+TGG	0.413971	4.3:+88603688	MS.gene000609:CDS
TCCTTTCATTGCTATGGATA+CGG	0.427277	4.3:+88603129	MS.gene000609:CDS
GGTATTGTTCTTCGACCTGT+TGG	0.437734	4.3:+88603160	MS.gene000609:CDS
ATGTTAAAGCTTATACAATT+GGG	0.439850	4.3:+88603238	MS.gene000609:CDS
GATTTGTCATCAAGCGGGTT+CGG	0.446291	4.3:+88603711	MS.gene000609:CDS
GAATAAGCTTGCAGAGTTGT+TGG	0.449673	4.3:+88603672	MS.gene000609:CDS
GCAGGGGTCTTGTATGGGTT+AGG	0.460666	4.3:+88603805	MS.gene000609:CDS
GTATTGTTCTTCGACCTGTT+GGG	0.461121	4.3:+88603161	MS.gene000609:CDS
AGAGTTGTTGGAGGTTAAGA+GGG	0.462475	4.3:+88603684	MS.gene000609:CDS
TAGAGAATTTAATGTTAATG+AGG	0.462708	4.3:+88603339	MS.gene000609:CDS
AATTTACCTGATACACAAGT+TGG	0.472874	4.3:+88603562	MS.gene000609:CDS
ATTTACCTGATACACAAGTT+GGG	0.507490	4.3:+88603563	MS.gene000609:CDS
GGATCTTTGGAGAAATATGC+AGG	0.509118	4.3:+88603787	MS.gene000609:CDS
CTATCATCTTCGCCGCAAGT+TGG	0.526576	4.3:-88603292	None:intergenic
GGTTATCGCTCCAAACTTCA+CGG	0.529023	4.3:-88603063	None:intergenic
TTTGTAATAGTCTTCATGGT+GGG	0.534821	4.3:+88603647	MS.gene000609:CDS
GTTTGATATCATACAGCATA+GGG	0.539432	4.3:-88603612	None:intergenic
TATGGGTTAGGTGTTGAGAA+TGG	0.543159	4.3:+88603817	MS.gene000609:CDS
ATGAACATGGTATCGATGCT+AGG	0.555439	4.3:+88603425	MS.gene000609:CDS
TAAGCTTGCAGAGTTGTTGG+AGG	0.562547	4.3:+88603675	MS.gene000609:CDS
TGTTTGATATCATACAGCAT+AGG	0.581668	4.3:-88603613	None:intergenic
ACAGGTCGAAGAACAATACC+AGG	0.581710	4.3:-88603157	None:intergenic
CATGGAAATCTTCCAACTTG+CGG	0.583679	4.3:+88603280	MS.gene000609:CDS
TCCGTATCCATAGCAATGAA+AGG	0.583831	4.3:-88603130	None:intergenic
GTTGGGATTTGTCATCAAGC+GGG	0.588282	4.3:+88603706	MS.gene000609:CDS
AATATGCAGGGGTCTTGTAT+GGG	0.597633	4.3:+88603800	MS.gene000609:CDS
GGTTGGGATTTGTCATCAAG+CGG	0.598328	4.3:+88603705	MS.gene000609:CDS
GCTATGGATACGGAATTCCC+TGG	0.614374	4.3:+88603139	MS.gene000609:CDS
GATCTTTGGAGAAATATGCA+GGG	0.629100	4.3:+88603788	MS.gene000609:CDS
ATCTTTGGAGAAATATGCAG+GGG	0.683756	4.3:+88603789	MS.gene000609:CDS
CAGGTCGAAGAACAATACCA+GGG	0.748306	4.3:-88603156	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGTTAAAGCTTATACAATT+GGG	+	chr4.3:88603238-88603257	MS.gene000609:CDS	20.0%
!!	TAGAGAATTTAATGTTAATG+AGG	+	chr4.3:88603339-88603358	MS.gene000609:CDS	20.0%
!!	TATGTTAAAGCTTATACAAT+TGG	+	chr4.3:88603237-88603256	MS.gene000609:CDS	20.0%
!!	TTCAAGAAAAATAATGAACA+TGG	+	chr4.3:88603412-88603431	MS.gene000609:CDS	20.0%
!!!	ACATATCAACATTATCTTTT+AGG	-	chr4.3:88603222-88603241	None:intergenic	20.0%
!!!	CATATCAACATTATCTTTTA+GGG	-	chr4.3:88603221-88603240	None:intergenic	20.0%
!!!	TTTTGAATGATAATGTTCAT+TGG	+	chr4.3:88603482-88603501	MS.gene000609:CDS	20.0%
!	TTGAAAGAGAATTTCTTTAG+TGG	+	chr4.3:88603766-88603785	MS.gene000609:CDS	25.0%
!!!	AAGTTTTGTAATAGTCTTCA+TGG	+	chr4.3:88603643-88603662	MS.gene000609:CDS	25.0%
!!!	TTTCATAGTGGATATGATTT+TGG	+	chr4.3:88603511-88603530	MS.gene000609:CDS	25.0%
	AATTTACCTGATACACAAGT+TGG	+	chr4.3:88603562-88603581	MS.gene000609:CDS	30.0%
	ATTTACCTGATACACAAGTT+GGG	+	chr4.3:88603563-88603582	MS.gene000609:CDS	30.0%
	GTTTGATATCATACAGCATA+GGG	-	chr4.3:88603615-88603634	None:intergenic	30.0%
	TGATTATCCTTTCATTGCTA+TGG	+	chr4.3:88603123-88603142	MS.gene000609:CDS	30.0%
	TGTTTGATATCATACAGCAT+AGG	-	chr4.3:88603616-88603635	None:intergenic	30.0%
	TTTGTAATAGTCTTCATGGT+GGG	+	chr4.3:88603647-88603666	MS.gene000609:CDS	30.0%
!	CATTGGATAACTTTTCATAG+TGG	+	chr4.3:88603499-88603518	MS.gene000609:CDS	30.0%
!	GAATTTCTTTAGTGGATCTT+TGG	+	chr4.3:88603774-88603793	MS.gene000609:CDS	30.0%
!	TTTTGTAATAGTCTTCATGG+TGG	+	chr4.3:88603646-88603665	MS.gene000609:CDS	30.0%
!!	AAGATGATAGGTTTTGTATC+TGG	+	chr4.3:88603305-88603324	MS.gene000609:CDS	30.0%
!!!	CAACATTATCTTTTAGGGTT+TGG	-	chr4.3:88603216-88603235	None:intergenic	30.0%
!!!	TCGTTTTGAGGTAAAATTAG+CGG	-	chr4.3:88603028-88603047	None:intergenic	30.0%
	TCCTTTCATTGCTATGGATA+CGG	+	chr4.3:88603129-88603148	MS.gene000609:CDS	35.0%
!	ATCTTTGGAGAAATATGCAG+GGG	+	chr4.3:88603789-88603808	MS.gene000609:CDS	35.0%
!	GATCTTTGGAGAAATATGCA+GGG	+	chr4.3:88603788-88603807	MS.gene000609:CDS	35.0%
!!!	ATTGAGCTTTTGAGACAAAG+TGG	+	chr4.3:88603382-88603401	MS.gene000609:CDS	35.0%
!!!	CTTACTTTTTCTGATGAGCA+TGG	+	chr4.3:88603262-88603281	MS.gene000609:CDS	35.0%
!!!	TTGCTTTTGAAATTCCCAAC+AGG	-	chr4.3:88603178-88603197	None:intergenic	35.0%
	AAGAACCCAACTTGTGTATC+AGG	-	chr4.3:88603571-88603590	None:intergenic	40.0%
	AGAGTTGTTGGAGGTTAAGA+GGG	+	chr4.3:88603684-88603703	MS.gene000609:CDS	40.0%
	CATGGAAATCTTCCAACTTG+CGG	+	chr4.3:88603280-88603299	MS.gene000609:CDS	40.0%
	GAATAAGCTTGCAGAGTTGT+TGG	+	chr4.3:88603672-88603691	MS.gene000609:CDS	40.0%
	GTATTGTTCTTCGACCTGTT+GGG	+	chr4.3:88603161-88603180	MS.gene000609:CDS	40.0%
	TACTCACATCTTCTGCATTC+AGG	+	chr4.3:88603740-88603759	MS.gene000609:CDS	40.0%
	TCACAGCGAATTCTTCTTCA+AGG	-	chr4.3:88603087-88603106	None:intergenic	40.0%
	TCCGTATCCATAGCAATGAA+AGG	-	chr4.3:88603133-88603152	None:intergenic	40.0%
!	AAATATGCAGGGGTCTTGTA+TGG	+	chr4.3:88603799-88603818	MS.gene000609:CDS	40.0%
!	AATATGCAGGGGTCTTGTAT+GGG	+	chr4.3:88603800-88603819	MS.gene000609:CDS	40.0%
!	GGATCTTTGGAGAAATATGC+AGG	+	chr4.3:88603787-88603806	MS.gene000609:CDS	40.0%
!	GTGAGCTTTTGATGTCATCT+GGG	+	chr4.3:88603455-88603474	MS.gene000609:CDS	40.0%
!!	ATGAACATGGTATCGATGCT+AGG	+	chr4.3:88603425-88603444	MS.gene000609:CDS	40.0%
!!	TATGGGTTAGGTGTTGAGAA+TGG	+	chr4.3:88603817-88603836	MS.gene000609:CDS	40.0%
!!	TGAATCGAATCGTCGTTTTG+AGG	-	chr4.3:88603040-88603059	None:intergenic	40.0%
	ACAGGTCGAAGAACAATACC+AGG	-	chr4.3:88603160-88603179	None:intergenic	45.0%
	CAGAGTTGTTGGAGGTTAAG+AGG	+	chr4.3:88603683-88603702	MS.gene000609:CDS	45.0%
	CAGGTCGAAGAACAATACCA+GGG	-	chr4.3:88603159-88603178	None:intergenic	45.0%
	CGATTCAGATCCGTGAAGTT+TGG	+	chr4.3:88603053-88603072	MS.gene000609:CDS	45.0%
	GGTATTGTTCTTCGACCTGT+TGG	+	chr4.3:88603160-88603179	MS.gene000609:CDS	45.0%
	GGTTATCGCTCCAAACTTCA+CGG	-	chr4.3:88603066-88603085	None:intergenic	45.0%
	GGTTGGGATTTGTCATCAAG+CGG	+	chr4.3:88603705-88603724	MS.gene000609:CDS	45.0%
	GTTGGGATTTGTCATCAAGC+GGG	+	chr4.3:88603706-88603725	MS.gene000609:CDS	45.0%
	TAAGCTTGCAGAGTTGTTGG+AGG	+	chr4.3:88603675-88603694	MS.gene000609:CDS	45.0%
	TGTTGGAGGTTAAGAGGGTT+GGG	+	chr4.3:88603689-88603708	MS.gene000609:CDS	45.0%
	TTGTTGGAGGTTAAGAGGGT+TGG	+	chr4.3:88603688-88603707	MS.gene000609:CDS	45.0%
!	GATTTGTCATCAAGCGGGTT+CGG	+	chr4.3:88603711-88603730	MS.gene000609:CDS	45.0%
!	GGTGAGCTTTTGATGTCATC+TGG	+	chr4.3:88603454-88603473	MS.gene000609:CDS	45.0%
	CAACTTGCGGCGAAGATGAT+AGG	+	chr4.3:88603293-88603312	MS.gene000609:CDS	50.0%
	CTATCATCTTCGCCGCAAGT+TGG	-	chr4.3:88603295-88603314	None:intergenic	50.0%
	GCTATGGATACGGAATTCCC+TGG	+	chr4.3:88603139-88603158	MS.gene000609:CDS	50.0%
!!	GGTATCGATGCTAGGCGTTT+CGG	+	chr4.3:88603433-88603452	MS.gene000609:CDS	50.0%
!	GCAGGGGTCTTGTATGGGTT+AGG	+	chr4.3:88603805-88603824	MS.gene000609:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	88603022	88603855	88603022	ID=MS.gene000609
chr4.3	mRNA	88603022	88603855	88603022	ID=MS.gene000609.t1;Parent=MS.gene000609
chr4.3	exon	88603022	88603855	88603022	ID=MS.gene000609.t1.exon1;Parent=MS.gene000609.t1
chr4.3	CDS	88603022	88603855	88603022	ID=cds.MS.gene000609.t1;Parent=MS.gene000609.t1

Gene Sequence

>MS.gene000609

ATGCCGCTAATTTTACCTCAAAACGACGATTCGATTCAGATCCGTGAAGTTTGGAGCGATAACCTTGAAGAAGAATTCGCTGTGATTCGAGAAATTGTTGATGATTATCCTTTCATTGCTATGGATACGGAATTCCCTGGTATTGTTCTTCGACCTGTTGGGAATTTCAAAAGCAATTATGATTATCATTACCAAACCCTAAAAGATAATGTTGATATGTTAAAGCTTATACAATTGGGTCTTACTTTTTCTGATGAGCATGGAAATCTTCCAACTTGCGGCGAAGATGATAGGTTTTGTATCTGGCAATTCAATTTTAGAGAATTTAATGTTAATGAGGATGTTTTTGCAAATGATTCAATTGAGCTTTTGAGACAAAGTGGAATTGATTTCAAGAAAAATAATGAACATGGTATCGATGCTAGGCGTTTCGGTGAGCTTTTGATGTCATCTGGGATTGTTTTGAATGATAATGTTCATTGGATAACTTTTCATAGTGGATATGATTTTGGTTATTTGTTGAAGTTGTTGACTTGTCAAAATTTACCTGATACACAAGTTGGGTTCTTTAATTTGATTAACATGTATTTCCCTATGCTGTATGATATCAAACATTTGATGAAGTTTTGTAATAGTCTTCATGGTGGGTTGAATAAGCTTGCAGAGTTGTTGGAGGTTAAGAGGGTTGGGATTTGTCATCAAGCGGGTTCGGATAGTTTACTCACATCTTCTGCATTCAGGAAATTGAAAGAGAATTTCTTTAGTGGATCTTTGGAGAAATATGCAGGGGTCTTGTATGGGTTAGGTGTTGAGAATGGACAGAGTACTCATTAA

Protein sequence

>MS.gene000609.t1

MPLILPQNDDSIQIREVWSDNLEEEFAVIREIVDDYPFIAMDTEFPGIVLRPVGNFKSNYDYHYQTLKDNVDMLKLIQLGLTFSDEHGNLPTCGEDDRFCIWQFNFREFNVNEDVFANDSIELLRQSGIDFKKNNEHGIDARRFGELLMSSGIVLNDNVHWITFHSGYDFGYLLKLLTCQNLPDTQVGFFNLINMYFPMLYDIKHLMKFCNSLHGGLNKLAELLEVKRVGICHQAGSDSLLTSSAFRKLKENFFSGSLEKYAGVLYGLGVENGQSTH