Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025628.t1 | XP_024625187.1 | 96.6 | 235 | 8 | 0 | 1 | 235 | 52 | 286 | 9.80E-128 | 466.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025628.t1 | Q9FMS6 | 65.5 | 238 | 77 | 4 | 1 | 235 | 41 | 276 | 4.1e-82 | 305.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025628.t1 | A0A396H7H5 | 96.6 | 235 | 8 | 0 | 1 | 235 | 52 | 286 | 7.1e-128 | 466.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene025628.t1 | AT5G22250 | 65.546 | 238 | 77 | 4 | 1 | 235 | 41 | 276 | 2.96e-105 | 305 |
| MS.gene025628.t1 | AT3G44260 | 65.126 | 238 | 75 | 5 | 1 | 235 | 46 | 278 | 1.61e-101 | 296 |
| MS.gene025628.t1 | AT5G10960 | 54.701 | 234 | 99 | 4 | 1 | 234 | 39 | 265 | 1.14e-81 | 246 |
| MS.gene025628.t1 | AT2G32070 | 54.043 | 235 | 100 | 5 | 1 | 234 | 39 | 266 | 1.59e-81 | 245 |
| MS.gene025628.t1 | AT1G15920 | 50.840 | 238 | 110 | 4 | 1 | 235 | 43 | 276 | 1.80e-80 | 243 |
| MS.gene025628.t1 | AT1G15920 | 50.840 | 238 | 110 | 4 | 1 | 235 | 43 | 276 | 1.80e-80 | 243 |
| MS.gene025628.t1 | AT1G80780 | 52.564 | 234 | 104 | 4 | 1 | 234 | 39 | 265 | 8.73e-79 | 238 |
| MS.gene025628.t1 | AT1G80780 | 52.564 | 234 | 104 | 4 | 1 | 234 | 39 | 265 | 8.73e-79 | 238 |
| MS.gene025628.t1 | AT1G80780 | 52.564 | 234 | 104 | 4 | 1 | 234 | 39 | 265 | 1.23e-78 | 238 |
| MS.gene025628.t1 | AT1G06450 | 35.294 | 238 | 129 | 9 | 2 | 234 | 37 | 254 | 6.91e-33 | 122 |
| MS.gene025628.t1 | AT1G61470 | 34.404 | 218 | 120 | 6 | 1 | 213 | 29 | 228 | 1.73e-30 | 114 |
| MS.gene025628.t1 | AT1G27820 | 35.648 | 216 | 122 | 5 | 1 | 213 | 33 | 234 | 7.27e-30 | 113 |
| MS.gene025628.t1 | AT3G44240 | 34.884 | 215 | 123 | 7 | 1 | 213 | 16 | 215 | 8.46e-27 | 103 |
| MS.gene025628.t1 | AT1G27890 | 34.722 | 216 | 124 | 5 | 1 | 213 | 28 | 229 | 2.75e-26 | 103 |
Find 52 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCTGAAAATAGTTATATT+TGG | 0.169182 | 7.3:-8846496 | MS.gene025628:CDS |
| TTCATAGTGCGTATGATTTC+GGG | 0.187035 | 7.3:-8846289 | MS.gene025628:CDS |
| TTGAAGGTGGAGAGGCTTAT+AGG | 0.219054 | 7.3:-8846107 | MS.gene025628:CDS |
| TTTCATAGTGCGTATGATTT+CGG | 0.233508 | 7.3:-8846290 | MS.gene025628:CDS |
| TGAAGGTGGAGAGGCTTATA+GGG | 0.242741 | 7.3:-8846106 | MS.gene025628:CDS |
| TGCGTATGATTTCGGGTATT+TGG | 0.263514 | 7.3:-8846282 | MS.gene025628:CDS |
| GAAGCTGTTACCGGATGATT+TGG | 0.329254 | 7.3:-8846243 | MS.gene025628:CDS |
| TGCTGAAAATAGTTATATTT+GGG | 0.331814 | 7.3:-8846495 | MS.gene025628:CDS |
| TTTGTGCTGATTCTGTTAGC+TGG | 0.344952 | 7.3:-8846319 | MS.gene025628:CDS |
| TTAACTCTTTCTGATCATAA+AGG | 0.384845 | 7.3:-8846536 | MS.gene025628:CDS |
| GAAGGTGTATTTCGGTGATA+TGG | 0.431170 | 7.3:-8846201 | MS.gene025628:CDS |
| CTGATTCTGTTGAGTTGTTG+AGG | 0.433052 | 7.3:-8846427 | MS.gene025628:CDS |
| TCAATGGACACGGAGTTTCC+CGG | 0.451307 | 7.3:-8846668 | MS.gene025628:CDS |
| ATATCAGAGAGCTTTACTTC+GGG | 0.453384 | 7.3:-8846031 | MS.gene025628:CDS |
| AGGTTGAATTTGATTCAGAT+TGG | 0.457708 | 7.3:-8846560 | MS.gene025628:CDS |
| AGGCTGGTGAAGGTGTATTT+CGG | 0.458544 | 7.3:-8846209 | MS.gene025628:CDS |
| GGTTGAATTTGATTCAGATT+GGG | 0.466535 | 7.3:-8846559 | MS.gene025628:CDS |
| GTTGATGATGTCGTCCGGTT+TGG | 0.470458 | 7.3:-8846342 | MS.gene025628:CDS |
| ATTTGCATGGTGGATTGGAT+AGG | 0.470548 | 7.3:-8846142 | MS.gene025628:CDS |
| TGTTGAAAGCGAATGTTGAT+AGG | 0.470879 | 7.3:-8846580 | MS.gene025628:CDS |
| TACGAATTTGCATGGTGGAT+TGG | 0.478435 | 7.3:-8846147 | MS.gene025628:CDS |
| TTGTGCTGATTCTGTTAGCT+GGG | 0.481953 | 7.3:-8846318 | MS.gene025628:CDS |
| GTTCTTGAACGGCGAATCAC+AGG | 0.491118 | 7.3:+8846625 | None:intergenic |
| TTGAGAAGAATCGTGAGTGT+GGG | 0.492422 | 7.3:-8846388 | MS.gene025628:CDS |
| AATATCAGAGAGCTTTACTT+CGG | 0.499438 | 7.3:-8846032 | MS.gene025628:CDS |
| TAGGGTTTGCAAGAGTTTGA+AGG | 0.503822 | 7.3:-8846123 | MS.gene025628:CDS |
| GCTGACGCAGAAGCTGTTAC+CGG | 0.505236 | 7.3:-8846252 | MS.gene025628:CDS |
| TTTGCATGGTGGATTGGATA+GGG | 0.507227 | 7.3:-8846141 | MS.gene025628:CDS |
| AAATATGCCGGTGTATTGTA+TGG | 0.511609 | 7.3:-8845990 | MS.gene025628:CDS |
| AGTTTCCCGGTGTGATTGTG+AGG | 0.516131 | 7.3:-8846655 | MS.gene025628:CDS |
| AGAATCAGCACAAACCAAAC+CGG | 0.520363 | 7.3:+8846328 | None:intergenic |
| TTTACTTCGGGAAAGCAGAC+GGG | 0.525168 | 7.3:-8846019 | MS.gene025628:CDS |
| GGTTTGCAAGAGTTTGAAGG+TGG | 0.534617 | 7.3:-8846120 | MS.gene025628:CDS |
| CTTTACTTCGGGAAAGCAGA+CGG | 0.534703 | 7.3:-8846020 | MS.gene025628:CDS |
| GCTGTTACCGGATGATTTGG+AGG | 0.539995 | 7.3:-8846240 | MS.gene025628:CDS |
| GACGGGTTTGTCAAATATGC+CGG | 0.543121 | 7.3:-8846002 | MS.gene025628:CDS |
| GCTGAGTTGATGATGTCGTC+CGG | 0.548614 | 7.3:-8846347 | MS.gene025628:CDS |
| CACGCGCCACGGTTCTTGAA+CGG | 0.548659 | 7.3:+8846614 | None:intergenic |
| GTTGAGTTGTTGAGGAGACA+AGG | 0.563888 | 7.3:-8846419 | MS.gene025628:CDS |
| GTTTCCCGGTGTGATTGTGA+GGG | 0.568400 | 7.3:-8846654 | MS.gene025628:CDS |
| TTTGAGAAGAATCGTGAGTG+TGG | 0.568643 | 7.3:-8846389 | MS.gene025628:CDS |
| TCCCGGTGTGATTGTGAGGG+CGG | 0.571114 | 7.3:-8846651 | MS.gene025628:CDS |
| GCAAGAGTTTGAAGGTGGAG+AGG | 0.582586 | 7.3:-8846115 | MS.gene025628:CDS |
| TTGAGTTGTTGAGGAGACAA+GGG | 0.608645 | 7.3:-8846418 | MS.gene025628:CDS |
| TCGATGTGAAGCATTTGATG+AGG | 0.612916 | 7.3:-8846175 | MS.gene025628:CDS |
| TTCTAATCCATACAATACAC+CGG | 0.622880 | 7.3:+8845983 | None:intergenic |
| ATCCGCCCTCACAATCACAC+CGG | 0.632148 | 7.3:+8846649 | None:intergenic |
| TCCGCTAATTTCAATGGACA+CGG | 0.632987 | 7.3:-8846678 | None:intergenic |
| CTGCATAGAGACACGCGCCA+CGG | 0.638041 | 7.3:+8846603 | None:intergenic |
| TCCGCCCTCACAATCACACC+GGG | 0.638539 | 7.3:+8846650 | None:intergenic |
| ATAGGGAAGAGTCATCAAGC+AGG | 0.666021 | 7.3:-8846089 | MS.gene025628:CDS |
| GATTCGCCGTTCAAGAACCG+TGG | 0.687694 | 7.3:-8846620 | MS.gene025628:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGCTGAAAATAGTTATATT+TGG | - | chr7.3:8846152-8846171 | MS.gene025628:CDS | 20.0% |
| !! | TGCTGAAAATAGTTATATTT+GGG | - | chr7.3:8846153-8846172 | MS.gene025628:CDS | 20.0% |
| !!! | TAATTTTAAGGATTTTGATG+TGG | - | chr7.3:8846180-8846199 | MS.gene025628:CDS | 20.0% |
| !!! | TATTTGGGAGTTTAATTTTA+AGG | - | chr7.3:8846168-8846187 | MS.gene025628:CDS | 20.0% |
| ! | TTAACTCTTTCTGATCATAA+AGG | - | chr7.3:8846112-8846131 | MS.gene025628:CDS | 25.0% |
| AATATCAGAGAGCTTTACTT+CGG | - | chr7.3:8846616-8846635 | MS.gene025628:CDS | 30.0% | |
| AGGTTGAATTTGATTCAGAT+TGG | - | chr7.3:8846088-8846107 | MS.gene025628:CDS | 30.0% | |
| GGTTGAATTTGATTCAGATT+GGG | - | chr7.3:8846089-8846108 | MS.gene025628:CDS | 30.0% | |
| TTCTAATCCATACAATACAC+CGG | + | chr7.3:8846668-8846687 | None:intergenic | 30.0% | |
| TTTCATAGTGCGTATGATTT+CGG | - | chr7.3:8846358-8846377 | MS.gene025628:CDS | 30.0% | |
| ! | CTATTTTCAGCATCTAACAT+TGG | + | chr7.3:8846145-8846164 | None:intergenic | 30.0% |
| AAAAACTCCTCCAAATCATC+CGG | + | chr7.3:8846418-8846437 | None:intergenic | 35.0% | |
| AAATATGCCGGTGTATTGTA+TGG | - | chr7.3:8846658-8846677 | MS.gene025628:CDS | 35.0% | |
| ATATCAGAGAGCTTTACTTC+GGG | - | chr7.3:8846617-8846636 | MS.gene025628:CDS | 35.0% | |
| TGTTGAAAGCGAATGTTGAT+AGG | - | chr7.3:8846068-8846087 | MS.gene025628:CDS | 35.0% | |
| TTCATAGTGCGTATGATTTC+GGG | - | chr7.3:8846359-8846378 | MS.gene025628:CDS | 35.0% | |
| ! | TTTTGTACGAATTTGCATGG+TGG | - | chr7.3:8846496-8846515 | MS.gene025628:CDS | 35.0% |
| !! | AGGTTTTGTACGAATTTGCA+TGG | - | chr7.3:8846493-8846512 | MS.gene025628:CDS | 35.0% |
| !! | ATGATTTGGAGGAGTTTTTG+AGG | - | chr7.3:8846419-8846438 | MS.gene025628:CDS | 35.0% |
| AGAATCAGCACAAACCAAAC+CGG | + | chr7.3:8846323-8846342 | None:intergenic | 40.0% | |
| TAGGGTTTGCAAGAGTTTGA+AGG | - | chr7.3:8846525-8846544 | MS.gene025628:CDS | 40.0% | |
| TTGAGAAGAATCGTGAGTGT+GGG | - | chr7.3:8846260-8846279 | MS.gene025628:CDS | 40.0% | |
| TTTGAGAAGAATCGTGAGTG+TGG | - | chr7.3:8846259-8846278 | MS.gene025628:CDS | 40.0% | |
| ! | CTGATTCTGTTGAGTTGTTG+AGG | - | chr7.3:8846221-8846240 | MS.gene025628:CDS | 40.0% |
| ! | GAAGGTGTATTTCGGTGATA+TGG | - | chr7.3:8846447-8846466 | MS.gene025628:CDS | 40.0% |
| ! | TCGATGTGAAGCATTTGATG+AGG | - | chr7.3:8846473-8846492 | MS.gene025628:CDS | 40.0% |
| ! | TGCGTATGATTTCGGGTATT+TGG | - | chr7.3:8846366-8846385 | MS.gene025628:CDS | 40.0% |
| ! | TTGAGTTGTTGAGGAGACAA+GGG | - | chr7.3:8846230-8846249 | MS.gene025628:CDS | 40.0% |
| !! | ATTTGCATGGTGGATTGGAT+AGG | - | chr7.3:8846506-8846525 | MS.gene025628:CDS | 40.0% |
| !! | TACGAATTTGCATGGTGGAT+TGG | - | chr7.3:8846501-8846520 | MS.gene025628:CDS | 40.0% |
| !! | TTGTGCTGATTCTGTTAGCT+GGG | - | chr7.3:8846330-8846349 | MS.gene025628:CDS | 40.0% |
| !! | TTTGCATGGTGGATTGGATA+GGG | - | chr7.3:8846507-8846526 | MS.gene025628:CDS | 40.0% |
| !! | TTTGTGCTGATTCTGTTAGC+TGG | - | chr7.3:8846329-8846348 | MS.gene025628:CDS | 40.0% |
| ATAGGGAAGAGTCATCAAGC+AGG | - | chr7.3:8846559-8846578 | MS.gene025628:CDS | 45.0% | |
| CTTTACTTCGGGAAAGCAGA+CGG | - | chr7.3:8846628-8846647 | MS.gene025628:CDS | 45.0% | |
| GAAGCTGTTACCGGATGATT+TGG | - | chr7.3:8846405-8846424 | MS.gene025628:CDS | 45.0% | |
| GGTTTGCAAGAGTTTGAAGG+TGG | - | chr7.3:8846528-8846547 | MS.gene025628:CDS | 45.0% | |
| TGAAGGTGGAGAGGCTTATA+GGG | - | chr7.3:8846542-8846561 | MS.gene025628:CDS | 45.0% | |
| TTGAAGGTGGAGAGGCTTAT+AGG | - | chr7.3:8846541-8846560 | MS.gene025628:CDS | 45.0% | |
| TTTACTTCGGGAAAGCAGAC+GGG | - | chr7.3:8846629-8846648 | MS.gene025628:CDS | 45.0% | |
| ! | AGGCTGGTGAAGGTGTATTT+CGG | - | chr7.3:8846439-8846458 | MS.gene025628:CDS | 45.0% |
| ! | GACGGGTTTGTCAAATATGC+CGG | - | chr7.3:8846646-8846665 | MS.gene025628:CDS | 45.0% |
| ! | GTTGAGTTGTTGAGGAGACA+AGG | - | chr7.3:8846229-8846248 | MS.gene025628:CDS | 45.0% |
| !!! | TTTGGAGGAGTTTTTGAGGC+TGG | - | chr7.3:8846423-8846442 | MS.gene025628:CDS | 45.0% |
| AGTTTCCCGGTGTGATTGTG+AGG | - | chr7.3:8845993-8846012 | MS.gene025628:CDS | 50.0% | |
| GCAAGAGTTTGAAGGTGGAG+AGG | - | chr7.3:8846533-8846552 | MS.gene025628:CDS | 50.0% | |
| GTTCTTGAACGGCGAATCAC+AGG | + | chr7.3:8846026-8846045 | None:intergenic | 50.0% | |
| GTTTCCCGGTGTGATTGTGA+GGG | - | chr7.3:8845994-8846013 | MS.gene025628:CDS | 50.0% | |
| ! | GCTGTTACCGGATGATTTGG+AGG | - | chr7.3:8846408-8846427 | MS.gene025628:CDS | 50.0% |
| !! | GCTGAGTTGATGATGTCGTC+CGG | - | chr7.3:8846301-8846320 | MS.gene025628:CDS | 50.0% |
| !! | GTTGATGATGTCGTCCGGTT+TGG | - | chr7.3:8846306-8846325 | MS.gene025628:CDS | 50.0% |
| !!! | GGAGTTTTTGAGGCTGGTGA+AGG | - | chr7.3:8846429-8846448 | MS.gene025628:CDS | 50.0% |
| ATCCGCCCTCACAATCACAC+CGG | + | chr7.3:8846002-8846021 | None:intergenic | 55.0% | |
| GATTCGCCGTTCAAGAACCG+TGG | - | chr7.3:8846028-8846047 | MS.gene025628:CDS | 55.0% | |
| GCTGACGCAGAAGCTGTTAC+CGG | - | chr7.3:8846396-8846415 | MS.gene025628:CDS | 55.0% | |
| CACGCGCCACGGTTCTTGAA+CGG | + | chr7.3:8846037-8846056 | None:intergenic | 60.0% | |
| CTGCATAGAGACACGCGCCA+CGG | + | chr7.3:8846048-8846067 | None:intergenic | 60.0% | |
| TCCCGGTGTGATTGTGAGGG+CGG | - | chr7.3:8845997-8846016 | MS.gene025628:CDS | 60.0% | |
| TCCGCCCTCACAATCACACC+GGG | + | chr7.3:8846001-8846020 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 8845983 | 8846687 | 8845983 | ID=MS.gene025628 |
| chr7.3 | mRNA | 8845983 | 8846687 | 8845983 | ID=MS.gene025628.t1;Parent=MS.gene025628 |
| chr7.3 | exon | 8845983 | 8846687 | 8845983 | ID=MS.gene025628.t1.exon1;Parent=MS.gene025628.t1 |
| chr7.3 | CDS | 8845983 | 8846687 | 8845983 | ID=cds.MS.gene025628.t1;Parent=MS.gene025628.t1 |
| Gene Sequence |
| Protein sequence |
