AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21541


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21541.t1 AT5G22250 64.179 268 91 4 23 287 12 277 8.79e-119 342
MS.gene21541.t1 AT3G44260 63.941 269 89 5 22 287 16 279 4.89e-116 335
MS.gene21541.t1 AT5G10960 53.933 267 114 5 22 287 9 267 2.58e-93 277
MS.gene21541.t1 AT2G32070 52.809 267 118 5 22 287 9 268 3.83e-93 277
MS.gene21541.t1 AT1G15920 48.727 275 134 4 15 286 6 276 9.23e-92 274
MS.gene21541.t1 AT1G15920 48.727 275 134 4 15 286 6 276 9.23e-92 274
MS.gene21541.t1 AT1G80780 51.504 266 122 4 22 287 9 267 1.26e-89 268
MS.gene21541.t1 AT1G80780 51.504 266 122 4 22 287 9 267 1.26e-89 268
MS.gene21541.t1 AT1G80780 51.504 266 122 4 22 287 9 267 1.86e-89 268
MS.gene21541.t1 AT1G06450 35.581 267 147 9 24 285 8 254 5.04e-42 148
MS.gene21541.t1 AT1G61470 33.333 246 141 6 24 264 1 228 1.41e-33 124
MS.gene21541.t1 AT1G27820 34.553 246 144 5 22 264 3 234 2.56e-33 124
MS.gene21541.t1 AT1G27890 34.167 240 141 5 28 264 4 229 4.20e-30 115
MS.gene21541.t1 AT3G44240 34.649 228 132 7 39 264 3 215 6.77e-29 110

Find 63 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTGAAAATAGTTATATT+TGG 0.169182 7.3:-8816512 MS.gene21541:CDS
TTCATAGTGCGTATGATTTC+GGG 0.187035 7.3:-8816305 MS.gene21541:CDS
TTGAAGGTGGAGAGGCTTAT+AGG 0.219054 7.3:-8816123 MS.gene21541:CDS
TTTCATAGTGCGTATGATTT+CGG 0.233508 7.3:-8816306 MS.gene21541:CDS
TGAAGGTGGAGAGGCTTATA+GGG 0.242741 7.3:-8816122 MS.gene21541:CDS
TGCGTATGATTTCGGGTATT+TGG 0.263514 7.3:-8816298 MS.gene21541:CDS
TGTAAGAATGGAACCATTAC+TGG 0.287488 7.3:+8816782 None:intergenic
GAAGCTGTTACCGGATGATT+TGG 0.329254 7.3:-8816259 MS.gene21541:CDS
TGCTGAAAATAGTTATATTT+GGG 0.331814 7.3:-8816511 MS.gene21541:CDS
TTTGTGCTGATTCTGTTAGC+TGG 0.344952 7.3:-8816335 MS.gene21541:CDS
TCAAATCTTGATAACGAATT+CGG 0.346517 7.3:-8816744 MS.gene21541:CDS
TTAACTCTTTCTGATCATAA+AGG 0.384845 7.3:-8816552 MS.gene21541:CDS
GAAGGTGTATTTCGGTGATA+TGG 0.431170 7.3:-8816217 MS.gene21541:CDS
CTGATTCTGTTGAGTTGTTG+AGG 0.433052 7.3:-8816443 MS.gene21541:CDS
AAAGAGGAATCGATTCCGTT+AGG 0.437401 7.3:+8816814 None:intergenic
TCAATGGACACGGAGTTTCC+CGG 0.451307 7.3:-8816684 MS.gene21541:CDS
ATATCAGAGAGCTTTACTTC+GGG 0.453384 7.3:-8816047 MS.gene21541:CDS
AGGTTGAATTTGATTCAGAT+TGG 0.457708 7.3:-8816576 MS.gene21541:CDS
AGGCTGGTGAAGGTGTATTT+CGG 0.458544 7.3:-8816225 MS.gene21541:CDS
CCACACTGATCTTGTAAGAA+TGG 0.461921 7.3:+8816770 None:intergenic
GGTTGAATTTGATTCAGATT+GGG 0.466535 7.3:-8816575 MS.gene21541:CDS
GTTGATGATGTCGTCCGGTT+TGG 0.470458 7.3:-8816358 MS.gene21541:CDS
ATTTGCATGGTGGATTGGAT+AGG 0.470548 7.3:-8816158 MS.gene21541:CDS
TGTTGAAAGCGAATGTTGAT+AGG 0.470879 7.3:-8816596 MS.gene21541:CDS
AAGAGGAATCGATTCCGTTA+GGG 0.475020 7.3:+8816815 None:intergenic
TACGAATTTGCATGGTGGAT+TGG 0.478435 7.3:-8816163 MS.gene21541:CDS
TTGTGCTGATTCTGTTAGCT+GGG 0.481953 7.3:-8816334 MS.gene21541:CDS
TCAGTATCCGCTAATTTCAA+TGG 0.486009 7.3:-8816700 MS.gene21541:CDS
GTTCTTGAACGGCGAATCAC+AGG 0.491118 7.3:+8816641 None:intergenic
CTTTCACCACGATCCAGTAA+TGG 0.491979 7.3:-8816795 MS.gene21541:CDS
TTGAGAAGAATCGTGAGTGT+GGG 0.492422 7.3:-8816404 MS.gene21541:CDS
AATATCAGAGAGCTTTACTT+CGG 0.499438 7.3:-8816048 MS.gene21541:CDS
TAGGGTTTGCAAGAGTTTGA+AGG 0.503822 7.3:-8816139 MS.gene21541:CDS
GCTGACGCAGAAGCTGTTAC+CGG 0.505236 7.3:-8816268 MS.gene21541:CDS
TTTGCATGGTGGATTGGATA+GGG 0.507227 7.3:-8816157 MS.gene21541:CDS
AAATATGCCGGTGTATTGTA+TGG 0.511609 7.3:-8816006 MS.gene21541:CDS
AGTTTCCCGGTGTGATTGTG+AGG 0.516131 7.3:-8816671 MS.gene21541:CDS
AGAATCAGCACAAACCAAAC+CGG 0.520363 7.3:+8816344 None:intergenic
TTTACTTCGGGAAAGCAGAC+GGG 0.525168 7.3:-8816035 MS.gene21541:CDS
GGTTTGCAAGAGTTTGAAGG+TGG 0.534617 7.3:-8816136 MS.gene21541:CDS
CTTTACTTCGGGAAAGCAGA+CGG 0.534703 7.3:-8816036 MS.gene21541:CDS
GCTGTTACCGGATGATTTGG+AGG 0.539995 7.3:-8816256 MS.gene21541:CDS
GACGGGTTTGTCAAATATGC+CGG 0.543121 7.3:-8816018 MS.gene21541:CDS
GCTGAGTTGATGATGTCGTC+CGG 0.548614 7.3:-8816363 MS.gene21541:CDS
CACGCGCCACGGTTCTTGAA+CGG 0.548659 7.3:+8816630 None:intergenic
TTACTGGATCGTGGTGAAAG+AGG 0.558261 7.3:+8816798 None:intergenic
GTTGAGTTGTTGAGGAGACA+AGG 0.563888 7.3:-8816435 MS.gene21541:CDS
GTTTCCCGGTGTGATTGTGA+GGG 0.568400 7.3:-8816670 MS.gene21541:CDS
TTTGAGAAGAATCGTGAGTG+TGG 0.568643 7.3:-8816405 MS.gene21541:CDS
TCCCGGTGTGATTGTGAGGG+CGG 0.571114 7.3:-8816667 MS.gene21541:CDS
GCAAGAGTTTGAAGGTGGAG+AGG 0.582586 7.3:-8816131 MS.gene21541:CDS
TCCGTGTCCATTGAAATTAG+CGG 0.591513 7.3:+8816693 None:intergenic
TTGAGTTGTTGAGGAGACAA+GGG 0.608645 7.3:-8816434 MS.gene21541:CDS
TCGATGTGAAGCATTTGATG+AGG 0.612916 7.3:-8816191 MS.gene21541:CDS
TTCTAATCCATACAATACAC+CGG 0.622880 7.3:+8815999 None:intergenic
ATCCGCCCTCACAATCACAC+CGG 0.632148 7.3:+8816665 None:intergenic
TCCGCTAATTTCAATGGACA+CGG 0.632987 7.3:-8816694 MS.gene21541:CDS
CTGCATAGAGACACGCGCCA+CGG 0.638041 7.3:+8816619 None:intergenic
TCCGCCCTCACAATCACACC+GGG 0.638539 7.3:+8816666 None:intergenic
ATAGGGAAGAGTCATCAAGC+AGG 0.666021 7.3:-8816105 MS.gene21541:CDS
ATGGAACCATTACTGGATCG+TGG 0.673080 7.3:+8816789 None:intergenic
GATTCGCCGTTCAAGAACCG+TGG 0.687694 7.3:-8816636 MS.gene21541:CDS
CCATTCTTACAAGATCAGTG+TGG 0.730924 7.3:-8816770 MS.gene21541:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGCTGAAAATAGTTATATT+TGG - chr7.3:8816312-8816331 MS.gene21541:CDS 20.0%
!! TGCTGAAAATAGTTATATTT+GGG - chr7.3:8816313-8816332 MS.gene21541:CDS 20.0%
!!! TAATTTTAAGGATTTTGATG+TGG - chr7.3:8816340-8816359 MS.gene21541:CDS 20.0%
!!! TATTTGGGAGTTTAATTTTA+AGG - chr7.3:8816328-8816347 MS.gene21541:CDS 20.0%
! TTAACTCTTTCTGATCATAA+AGG - chr7.3:8816272-8816291 MS.gene21541:CDS 25.0%
!!! TCAAATCTTGATAACGAATT+CGG - chr7.3:8816080-8816099 MS.gene21541:CDS 25.0%
AATATCAGAGAGCTTTACTT+CGG - chr7.3:8816776-8816795 MS.gene21541:CDS 30.0%
AGGTTGAATTTGATTCAGAT+TGG - chr7.3:8816248-8816267 MS.gene21541:CDS 30.0%
GGTTGAATTTGATTCAGATT+GGG - chr7.3:8816249-8816268 MS.gene21541:CDS 30.0%
TTCTAATCCATACAATACAC+CGG + chr7.3:8816828-8816847 None:intergenic 30.0%
TTTCATAGTGCGTATGATTT+CGG - chr7.3:8816518-8816537 MS.gene21541:CDS 30.0%
! CTATTTTCAGCATCTAACAT+TGG + chr7.3:8816305-8816324 None:intergenic 30.0%
AAAAACTCCTCCAAATCATC+CGG + chr7.3:8816578-8816597 None:intergenic 35.0%
AAATATGCCGGTGTATTGTA+TGG - chr7.3:8816818-8816837 MS.gene21541:CDS 35.0%
ATATCAGAGAGCTTTACTTC+GGG - chr7.3:8816777-8816796 MS.gene21541:CDS 35.0%
TAACCGCAAAAAAACCCTAA+CGG - chr7.3:8815995-8816014 MS.gene21541:CDS 35.0%
TCAGTATCCGCTAATTTCAA+TGG - chr7.3:8816124-8816143 MS.gene21541:CDS 35.0%
TGTAAGAATGGAACCATTAC+TGG + chr7.3:8816045-8816064 None:intergenic 35.0%
TGTTGAAAGCGAATGTTGAT+AGG - chr7.3:8816228-8816247 MS.gene21541:CDS 35.0%
TTCATAGTGCGTATGATTTC+GGG - chr7.3:8816519-8816538 MS.gene21541:CDS 35.0%
! TTTTGTACGAATTTGCATGG+TGG - chr7.3:8816656-8816675 MS.gene21541:CDS 35.0%
!! AGGTTTTGTACGAATTTGCA+TGG - chr7.3:8816653-8816672 MS.gene21541:CDS 35.0%
!! ATGATTTGGAGGAGTTTTTG+AGG - chr7.3:8816579-8816598 MS.gene21541:CDS 35.0%
!!! ATTCCGTTAGGGTTTTTTTG+CGG + chr7.3:8816001-8816020 None:intergenic 35.0%
AGAATCAGCACAAACCAAAC+CGG + chr7.3:8816483-8816502 None:intergenic 40.0%
CCACACTGATCTTGTAAGAA+TGG + chr7.3:8816057-8816076 None:intergenic 40.0%
CCATTCTTACAAGATCAGTG+TGG - chr7.3:8816054-8816073 MS.gene21541:CDS 40.0%
TAGGGTTTGCAAGAGTTTGA+AGG - chr7.3:8816685-8816704 MS.gene21541:CDS 40.0%
TCCGCTAATTTCAATGGACA+CGG - chr7.3:8816130-8816149 MS.gene21541:CDS 40.0%
TCCGTGTCCATTGAAATTAG+CGG + chr7.3:8816134-8816153 None:intergenic 40.0%
TTGAGAAGAATCGTGAGTGT+GGG - chr7.3:8816420-8816439 MS.gene21541:CDS 40.0%
TTTGAGAAGAATCGTGAGTG+TGG - chr7.3:8816419-8816438 MS.gene21541:CDS 40.0%
! AAAGAGGAATCGATTCCGTT+AGG + chr7.3:8816013-8816032 None:intergenic 40.0%
! AAGAGGAATCGATTCCGTTA+GGG + chr7.3:8816012-8816031 None:intergenic 40.0%
! CTGATTCTGTTGAGTTGTTG+AGG - chr7.3:8816381-8816400 MS.gene21541:CDS 40.0%
! GAAGGTGTATTTCGGTGATA+TGG - chr7.3:8816607-8816626 MS.gene21541:CDS 40.0%
! TCGATGTGAAGCATTTGATG+AGG - chr7.3:8816633-8816652 MS.gene21541:CDS 40.0%
! TGCGTATGATTTCGGGTATT+TGG - chr7.3:8816526-8816545 MS.gene21541:CDS 40.0%
! TTGAGTTGTTGAGGAGACAA+GGG - chr7.3:8816390-8816409 MS.gene21541:CDS 40.0%
!! ATTTGCATGGTGGATTGGAT+AGG - chr7.3:8816666-8816685 MS.gene21541:CDS 40.0%
!! TACGAATTTGCATGGTGGAT+TGG - chr7.3:8816661-8816680 MS.gene21541:CDS 40.0%
!! TTGTGCTGATTCTGTTAGCT+GGG - chr7.3:8816490-8816509 MS.gene21541:CDS 40.0%
!! TTTGCATGGTGGATTGGATA+GGG - chr7.3:8816667-8816686 MS.gene21541:CDS 40.0%
!! TTTGTGCTGATTCTGTTAGC+TGG - chr7.3:8816489-8816508 MS.gene21541:CDS 40.0%
ATAGGGAAGAGTCATCAAGC+AGG - chr7.3:8816719-8816738 MS.gene21541:CDS 45.0%
ATGGAACCATTACTGGATCG+TGG + chr7.3:8816038-8816057 None:intergenic 45.0%
CTTTACTTCGGGAAAGCAGA+CGG - chr7.3:8816788-8816807 MS.gene21541:CDS 45.0%
CTTTCACCACGATCCAGTAA+TGG - chr7.3:8816029-8816048 MS.gene21541:CDS 45.0%
GAAGCTGTTACCGGATGATT+TGG - chr7.3:8816565-8816584 MS.gene21541:CDS 45.0%
GGTTTGCAAGAGTTTGAAGG+TGG - chr7.3:8816688-8816707 MS.gene21541:CDS 45.0%
TGAAGGTGGAGAGGCTTATA+GGG - chr7.3:8816702-8816721 MS.gene21541:CDS 45.0%
TTACTGGATCGTGGTGAAAG+AGG + chr7.3:8816029-8816048 None:intergenic 45.0%
TTGAAGGTGGAGAGGCTTAT+AGG - chr7.3:8816701-8816720 MS.gene21541:CDS 45.0%
TTTACTTCGGGAAAGCAGAC+GGG - chr7.3:8816789-8816808 MS.gene21541:CDS 45.0%
! AGGCTGGTGAAGGTGTATTT+CGG - chr7.3:8816599-8816618 MS.gene21541:CDS 45.0%
! GACGGGTTTGTCAAATATGC+CGG - chr7.3:8816806-8816825 MS.gene21541:CDS 45.0%
! GTTGAGTTGTTGAGGAGACA+AGG - chr7.3:8816389-8816408 MS.gene21541:CDS 45.0%
!!! TTTGGAGGAGTTTTTGAGGC+TGG - chr7.3:8816583-8816602 MS.gene21541:CDS 45.0%
AGTTTCCCGGTGTGATTGTG+AGG - chr7.3:8816153-8816172 MS.gene21541:CDS 50.0%
GCAAGAGTTTGAAGGTGGAG+AGG - chr7.3:8816693-8816712 MS.gene21541:CDS 50.0%
GTTCTTGAACGGCGAATCAC+AGG + chr7.3:8816186-8816205 None:intergenic 50.0%
GTTTCCCGGTGTGATTGTGA+GGG - chr7.3:8816154-8816173 MS.gene21541:CDS 50.0%
TCAATGGACACGGAGTTTCC+CGG - chr7.3:8816140-8816159 MS.gene21541:CDS 50.0%
! GCTGTTACCGGATGATTTGG+AGG - chr7.3:8816568-8816587 MS.gene21541:CDS 50.0%
!! GCTGAGTTGATGATGTCGTC+CGG - chr7.3:8816461-8816480 MS.gene21541:CDS 50.0%
!! GTTGATGATGTCGTCCGGTT+TGG - chr7.3:8816466-8816485 MS.gene21541:CDS 50.0%
!!! GGAGTTTTTGAGGCTGGTGA+AGG - chr7.3:8816589-8816608 MS.gene21541:CDS 50.0%
ATCCGCCCTCACAATCACAC+CGG + chr7.3:8816162-8816181 None:intergenic 55.0%
GATTCGCCGTTCAAGAACCG+TGG - chr7.3:8816188-8816207 MS.gene21541:CDS 55.0%
GCTGACGCAGAAGCTGTTAC+CGG - chr7.3:8816556-8816575 MS.gene21541:CDS 55.0%
CACGCGCCACGGTTCTTGAA+CGG + chr7.3:8816197-8816216 None:intergenic 60.0%
CTGCATAGAGACACGCGCCA+CGG + chr7.3:8816208-8816227 None:intergenic 60.0%
TCCCGGTGTGATTGTGAGGG+CGG - chr7.3:8816157-8816176 MS.gene21541:CDS 60.0%
TCCGCCCTCACAATCACACC+GGG + chr7.3:8816161-8816180 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 8815990 8816856 8815990 ID=MS.gene21541
chr7.3 mRNA 8815990 8816856 8815990 ID=MS.gene21541.t1;Parent=MS.gene21541
chr7.3 exon 8815990 8816856 8815990 ID=MS.gene21541.t1.exon1;Parent=MS.gene21541.t1
chr7.3 CDS 8815990 8816856 8815990 ID=cds.MS.gene21541.t1;Parent=MS.gene21541.t1
Gene Sequence

>MS.gene21541

ATGTGTAACCGCAAAAAAACCCTAACGGAATCGATTCCTCTTTCACCACGATCCAGTAATGGTTCCATTCTTACAAGATCAGTGTGGAGTTCAAATCTTGATAACGAATTCGGTTTGATTCGATCGTTAATCGATCAGTATCCGCTAATTTCAATGGACACGGAGTTTCCCGGTGTGATTGTGAGGGCGGATTCCTGTGATTCGCCGTTCAAGAACCGTGGCGCGTGTCTCTATGCAGTGTTGAAAGCGAATGTTGATAGGTTGAATTTGATTCAGATTGGGTTAACTCTTTCTGATCATAAAGGTAATCTTCCAATGTTAGATGCTGAAAATAGTTATATTTGGGAGTTTAATTTTAAGGATTTTGATGTGGCGCGTGATGATCACGCTGCTGATTCTGTTGAGTTGTTGAGGAGACAAGGGATTGATTTTGAGAAGAATCGTGAGTGTGGGATTGATTCTGTTAAGTTTGCTGAGTTGATGATGTCGTCCGGTTTGGTTTGTGCTGATTCTGTTAGCTGGGTAACGTTTCATAGTGCGTATGATTTCGGGTATTTGGTGAAATTGCTGACGCAGAAGCTGTTACCGGATGATTTGGAGGAGTTTTTGAGGCTGGTGAAGGTGTATTTCGGTGATATGGTATTCGATGTGAAGCATTTGATGAGGTTTTGTACGAATTTGCATGGTGGATTGGATAGGGTTTGCAAGAGTTTGAAGGTGGAGAGGCTTATAGGGAAGAGTCATCAAGCAGGTTCAGATAGTTTGCTTACTTTGCATGCTTTTCAGAATATCAGAGAGCTTTACTTCGGGAAAGCAGACGGGTTTGTCAAATATGCCGGTGTATTGTATGGATTAGAAGTTTGCTGA

Protein sequence

>MS.gene21541.t1

MCNRKKTLTESIPLSPRSSNGSILTRSVWSSNLDNEFGLIRSLIDQYPLISMDTEFPGVIVRADSCDSPFKNRGACLYAVLKANVDRLNLIQIGLTLSDHKGNLPMLDAENSYIWEFNFKDFDVARDDHAADSVELLRRQGIDFEKNRECGIDSVKFAELMMSSGLVCADSVSWVTFHSAYDFGYLVKLLTQKLLPDDLEEFLRLVKVYFGDMVFDVKHLMRFCTNLHGGLDRVCKSLKVERLIGKSHQAGSDSLLTLHAFQNIRELYFGKADGFVKYAGVLYGLEVC