AlfalfaGEDB | MS.gene034815

M. sativa cultivar XinJiangDaYe / MS.gene034815

KEGG: K12581
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04857.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034815.t1	XP_024633362.1	99	288	3	0	1	288	1	288	8.20E-161	576.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034815.t1	Q9FMS6	61.8	272	95	5	16	285	12	276	7.2e-89	328.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034815.t1	A0A396JQM4	77.1	332	32	1	1	288	1	332	2.2e-139	504.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene034815	MS.gene85905	PPI
MS.gene72178	MS.gene034815	PPI
MS.gene034815	MS.gene68205	PPI
MS.gene034815	MS.gene85904	PPI
MS.gene034815	MS.gene030414	PPI
MS.gene034815	MS.gene84309	PPI
MS.gene034815	MS.gene53121	PPI
MS.gene034815	MS.gene68798	PPI
MS.gene034815	MS.gene034438	PPI
MS.gene034250	MS.gene034815	PPI
MS.gene034815	MS.gene030415	PPI
MS.gene034815	MS.gene53119	PPI
MS.gene034815	MS.gene000609	PPI
MS.gene034815	MS.gene55324	PPI
MS.gene034815	MS.gene020895	PPI
MS.gene034815	MS.gene38057	PPI
MS.gene034815	MS.gene72157	PPI
MS.gene034815	MS.gene35444	PPI
MS.gene034815	MS.gene38201	PPI
MS.gene034815	MS.gene38199	PPI
MS.gene034815	MS.gene85901	PPI
MS.gene034815	MS.gene53123	PPI
MS.gene034815	MS.gene78556	PPI
MS.gene034815	MS.gene85903	PPI
MS.gene034815	MS.gene048793	PPI
MS.gene034815	MS.gene35431	PPI
MS.gene034815	MS.gene53120	PPI
MS.gene034815	MS.gene053225	PPI
MS.gene070320	MS.gene034815	PPI
MS.gene034815	MS.gene72160	PPI
MS.gene034815	MS.gene72156	PPI
MS.gene034815	MS.gene78557	PPI
MS.gene034815	MS.gene048794	PPI
MS.gene034815	MS.gene014264	PPI
MS.gene034815	MS.gene35692	PPI
MS.gene034815	MS.gene53122	PPI
MS.gene81219	MS.gene034815	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034815.t1	AT5G22250	61.765	272	95	5	16	285	12	276	1.98e-114	331
MS.gene034815.t1	AT3G44260	59.928	277	101	4	10	285	11	278	1.46e-112	326
MS.gene034815.t1	AT2G32070	55.185	270	109	5	15	284	9	266	5.90e-98	289
MS.gene034815.t1	AT5G10960	54.815	270	109	5	15	284	9	265	1.07e-94	281
MS.gene034815.t1	AT1G80780	53.333	270	113	5	15	284	9	265	5.54e-93	276
MS.gene034815.t1	AT1G80780	53.333	270	113	5	15	284	9	265	5.54e-93	276
MS.gene034815.t1	AT1G80780	53.333	270	113	5	15	284	9	265	7.29e-93	276
MS.gene034815.t1	AT1G15920	48.718	273	131	5	15	286	13	277	1.58e-85	258
MS.gene034815.t1	AT1G15920	48.718	273	131	5	15	286	13	277	1.58e-85	258
MS.gene034815.t1	AT1G61470	37.652	247	126	8	21	262	5	228	1.08e-38	137
MS.gene034815.t1	AT1G06450	33.579	271	149	9	19	284	10	254	1.11e-38	139
MS.gene034815.t1	AT1G27820	37.500	256	130	10	14	262	2	234	1.97e-36	132
MS.gene034815.t1	AT1G27890	36.948	249	127	9	21	262	4	229	2.14e-32	121
MS.gene034815.t1	AT3G44240	36.052	233	127	7	32	262	3	215	1.80e-31	117

Find 0 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTGAGAAAAACAAGGAATTT+GGG	-	8685:220174-220193	MS.gene034815:CDS	25.0%
!	TTTGAGAAAAACAAGGAATT+TGG	-	8685:220173-220192	MS.gene034815:CDS	25.0%
!!!	GATTGATTTTGAGAAAAACA+AGG	-	8685:220166-220185	MS.gene034815:CDS	25.0%
	AAGATCAAGGAAGTTTACTT+TGG	-	8685:220530-220549	MS.gene034815:CDS	30.0%
	AATCTTCCGAAATTGAGAAA+TGG	-	8685:220041-220060	MS.gene034815:CDS	30.0%
	AGATCAAGGAAGTTTACTTT+GGG	-	8685:220531-220550	MS.gene034815:CDS	30.0%
	AGTCTTAATATCATCCAAGT+CGG	-	8685:219993-220012	MS.gene034815:CDS	30.0%
	GAGTTTCCATTTCTCAATTT+CGG	+	8685:220050-220069	None:intergenic	30.0%
	TTTCATAGCGGTTATGATTT+CGG	-	8685:220272-220291	MS.gene034815:CDS	30.0%
!	TGTTTATGATGTGAAGCATT+TGG	-	8685:220382-220401	MS.gene034815:CDS	30.0%
!!	GTTGTATGGATTAGAAAATG+TGG	-	8685:220589-220608	MS.gene034815:CDS	30.0%
	AATCAGAGTTCAAACTCATC+CGG	-	8685:219829-219848	MS.gene034815:CDS	35.0%
	ATTGAATTGCTGAAGAGTCA+GGG	-	8685:220143-220162	MS.gene034815:CDS	35.0%
	ATTGAGAAATGGAAACTCTG+AGG	-	8685:220052-220071	MS.gene034815:CDS	35.0%
	ATTGAGATTGATGAAGTGGA+GGG	+	8685:219783-219802	None:intergenic	35.0%
	CGGTTATGATTTCGGATATT+TGG	-	8685:220280-220299	MS.gene034815:CDS	35.0%
	CTGACTTTATCTGATGTTGA+TGG	-	8685:220017-220036	MS.gene034815:CDS	35.0%
	TATTGAATTGCTGAAGAGTC+AGG	-	8685:220142-220161	MS.gene034815:CDS	35.0%
	TTCACATCCGATCATTTCAA+TGG	-	8685:219863-219882	MS.gene034815:CDS	35.0%
!	GTTTACTTTGGGAATGATGA+TGG	-	8685:220542-220561	MS.gene034815:CDS	35.0%
!	TGATTTCGGATATTTGGTGA+AGG	-	8685:220286-220305	MS.gene034815:CDS	35.0%
!!	ATTTGGCAAAGTTTTGTGAG+GGG	-	8685:220399-220418	MS.gene034815:CDS	35.0%
!!	CATTTGGCAAAGTTTTGTGA+GGG	-	8685:220398-220417	MS.gene034815:CDS	35.0%
!!	TCATGCTTTTAGGAAGATCA+AGG	-	8685:220517-220536	MS.gene034815:CDS	35.0%
	AAGTATGCTGGTGTGTTGTA+TGG	-	8685:220575-220594	MS.gene034815:CDS	40.0%
	GATTGAGATTGATGAAGTGG+AGG	+	8685:219784-219803	None:intergenic	40.0%
	TCGGATTGAGATTGATGAAG+TGG	+	8685:219787-219806	None:intergenic	40.0%
	TTCATCAATCTCAATCCGAC+CGG	-	8685:219788-219807	MS.gene034815:CDS	40.0%
	TTGAATTGCTGAAGAGTCAG+GGG	-	8685:220144-220163	MS.gene034815:CDS	40.0%
!	AGGAATTTGGGATTGACTCT+AGG	-	8685:220186-220205	MS.gene034815:CDS	40.0%
!	GTTGTATGGTGGTTTGGATA+GGG	-	8685:220421-220440	MS.gene034815:CDS	40.0%
!	TCGGTGTCCATTGAAATGAT+CGG	+	8685:219873-219892	None:intergenic	40.0%
!!	AAGTTTTGTGAGGGGTTGTA+TGG	-	8685:220407-220426	MS.gene034815:CDS	40.0%
!!	GCATTTGGCAAAGTTTTGTG+AGG	-	8685:220397-220416	MS.gene034815:CDS	40.0%
!!	GCTGAATTGATGATGAGTTC+CGG	-	8685:220215-220234	MS.gene034815:CDS	40.0%
!!	TGCTGGTTAGGGTTTTCTTT+GGG	-	8685:220354-220373	MS.gene034815:CDS	40.0%
!!!	TGCTGACTCTTCATGCTTTT+AGG	-	8685:220507-220526	MS.gene034815:CDS	40.0%
	AGAAACTCAGCAAGCTCTTC+AGG	+	8685:220332-220351	None:intergenic	45.0%
	AGCTGGGTGACATTTCATAG+CGG	-	8685:220260-220279	MS.gene034815:CDS	45.0%
	ATAAGTCAGTGATGCCGTCA+GGG	+	8685:219917-219936	None:intergenic	45.0%
	CAGATAAAGTCAGACCGACT+TGG	+	8685:220010-220029	None:intergenic	45.0%
	CTCCTCATTACACACAAGTC+CGG	+	8685:220237-220256	None:intergenic	45.0%
	GAAGATGCTGAATGTGGATC+GGG	-	8685:220448-220467	MS.gene034815:CDS	45.0%
	GATGTGAATCCACGAATGAC+CGG	+	8685:219851-219870	None:intergenic	45.0%
	GGAAGATGCTGAATGTGGAT+CGG	-	8685:220447-220466	MS.gene034815:CDS	45.0%
	GGTCAACTGATGAAGTATGC+TGG	-	8685:220563-220582	MS.gene034815:CDS	45.0%
	TCGAGATTGAATGACCACAC+CGG	+	8685:219810-219829	None:intergenic	45.0%
	TGTGTAATGAGGAGGTTAGC+TGG	-	8685:220243-220262	MS.gene034815:CDS	45.0%
	TTCCGGACTTGTGTGTAATG+AGG	-	8685:220232-220251	MS.gene034815:CDS	45.0%
!	ACTCTGAGGAGTCTCTGATT+TGG	-	8685:220066-220085	MS.gene034815:CDS	45.0%
!	ATGGTGGTTTGGATAGGGTT+GGG	-	8685:220426-220445	MS.gene034815:CDS	45.0%
!	CTCTGAGGAGTCTCTGATTT+GGG	-	8685:220067-220086	MS.gene034815:CDS	45.0%
!	GCTGGTTAGGGTTTTCTTTG+GGG	-	8685:220355-220374	MS.gene034815:CDS	45.0%
!	GGTTGTATGGTGGTTTGGAT+AGG	-	8685:220420-220439	MS.gene034815:CDS	45.0%
!	GTGTAATGAGGAGGTTAGCT+GGG	-	8685:220244-220263	MS.gene034815:CDS	45.0%
!	TATGGTGGTTTGGATAGGGT+TGG	-	8685:220425-220444	MS.gene034815:CDS	45.0%
!	TGAATGTGGATCGGGTTGTT+GGG	-	8685:220456-220475	MS.gene034815:CDS	45.0%
!	TTTTGTGAGGGGTTGTATGG+TGG	-	8685:220410-220429	MS.gene034815:CDS	45.0%
!!	TGAAGAGCTTGCTGAGTTTC+TGG	-	8685:220331-220350	MS.gene034815:CDS	45.0%
!!	TGAGTTTCTGGTGCTGGTTA+GGG	-	8685:220343-220362	MS.gene034815:CDS	45.0%
!!!	GTGCTGGTTAGGGTTTTCTT+TGG	-	8685:220353-220372	MS.gene034815:CDS	45.0%
	CAAACTCATCCGGTCATTCG+TGG	-	8685:219839-219858	MS.gene034815:CDS	50.0%
	CGGACTTGTGTGTAATGAGG+AGG	-	8685:220235-220254	MS.gene034815:CDS	50.0%
	GACTATCAACATTCGCCCTG+AGG	+	8685:219977-219996	None:intergenic	50.0%
	GATAAGTCAGTGATGCCGTC+AGG	+	8685:219918-219937	None:intergenic	50.0%
	GATTGAATGACCACACCGGT+CGG	+	8685:219806-219825	None:intergenic	50.0%
	GGTTGGGAAGATGCTGAATG+TGG	-	8685:220442-220461	MS.gene034815:CDS	50.0%
	GGTTGTTGGGAAGACTCATG+AGG	-	8685:220469-220488	MS.gene034815:CDS	50.0%
	TCAATGGACACCGAGTTTCC+CGG	-	8685:219879-219898	MS.gene034815:CDS	50.0%
	TGAGGGGTTGTATGGTGGTT+TGG	-	8685:220415-220434	MS.gene034815:CDS	50.0%
	TGTTGGGAAGACTCATGAGG+CGG	-	8685:220472-220491	MS.gene034815:CDS	50.0%
!	CTGAATGTGGATCGGGTTGT+TGG	-	8685:220455-220474	MS.gene034815:CDS	50.0%
!!	CTGAGTTTCTGGTGCTGGTT+AGG	-	8685:220342-220361	MS.gene034815:CDS	50.0%
	CAATCTCAATCCGACCGGTG+TGG	-	8685:219793-219812	MS.gene034815:CDS	55.0%
	GTTGGGAAGACTCATGAGGC+GGG	-	8685:220473-220492	MS.gene034815:CDS	55.0%
	TGCCCACTACTCTGTCCTCA+GGG	-	8685:219959-219978	MS.gene034815:CDS	55.0%
!!	GCTTGCTGAGTTTCTGGTGC+TGG	-	8685:220337-220356	MS.gene034815:CDS	55.0%
	AGTCAGTGATGCCGTCAGGG+AGG	+	8685:219914-219933	None:intergenic	60.0%
	CGCCCTGAGGACAGAGTAGT+GGG	+	8685:219964-219983	None:intergenic	60.0%
	CTGCCCACTACTCTGTCCTC+AGG	-	8685:219958-219977	MS.gene034815:CDS	60.0%
	GATGACGACGCCGGGAAACT+CGG	+	8685:219892-219911	None:intergenic	60.0%
	TCGCCCTGAGGACAGAGTAG+TGG	+	8685:219965-219984	None:intergenic	60.0%
!!	AGGACAGAGTAGTGGGCAGC+AGG	+	8685:219957-219976	None:intergenic	60.0%
!!	GACAGAGTAGTGGGCAGCAG+GGG	+	8685:219955-219974	None:intergenic	60.0%
!!	GGACAGAGTAGTGGGCAGCA+GGG	+	8685:219956-219975	None:intergenic	60.0%
	AGTGGGCAGCAGGGGTTCGA+TGG	+	8685:219947-219966	None:intergenic	65.0%
	GGCGTCGTCATCCTCCCTGA+CGG	-	8685:219900-219919	MS.gene034815:CDS	65.0%
	GTCAGGGAGGATGACGACGC+CGG	+	8685:219901-219920	None:intergenic	65.0%
	TCAGGGAGGATGACGACGCC+GGG	+	8685:219900-219919	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
8685	gene	219750	220616	219750	ID=MS.gene034815
8685	mRNA	219750	220616	219750	ID=MS.gene034815.t1;Parent=MS.gene034815
8685	exon	219750	220616	219750	ID=MS.gene034815.t1.exon1;Parent=MS.gene034815.t1
8685	CDS	219750	220616	219750	ID=cds.MS.gene034815.t1;Parent=MS.gene034815.t1

Gene Sequence

>MS.gene034815

ATGTTTTCACAAAATATGTTCTTTGCAAAACCCTCCACTTCATCAATCTCAATCCGACCGGTGTGGTCATTCAATCTCGAATCAGAGTTCAAACTCATCCGGTCATTCGTGGATTCACATCCGATCATTTCAATGGACACCGAGTTTCCCGGCGTCGTCATCCTCCCTGACGGCATCACTGACTTATCATCTTACCATCGAACCCCTGCTGCCCACTACTCTGTCCTCAGGGCGAATGTTGATAGTCTTAATATCATCCAAGTCGGTCTGACTTTATCTGATGTTGATGGTAATCTTCCGAAATTGAGAAATGGAAACTCTGAGGAGTCTCTGATTTGGGAATTCAATTTCAGTGATTTTGACTTCGAGCGTGATATTCACGCGCACGAGTCTATTGAATTGCTGAAGAGTCAGGGGATTGATTTTGAGAAAAACAAGGAATTTGGGATTGACTCTAGGAGATTTGCTGAATTGATGATGAGTTCCGGACTTGTGTGTAATGAGGAGGTTAGCTGGGTGACATTTCATAGCGGTTATGATTTCGGATATTTGGTGAAGGCGTTGACTCAATATGCTCTGCCTGAAGAGCTTGCTGAGTTTCTGGTGCTGGTTAGGGTTTTCTTTGGGGAGAGTGTTTATGATGTGAAGCATTTGGCAAAGTTTTGTGAGGGGTTGTATGGTGGTTTGGATAGGGTTGGGAAGATGCTGAATGTGGATCGGGTTGTTGGGAAGACTCATGAGGCGGGTTCTGATAGTTTGCTGACTCTTCATGCTTTTAGGAAGATCAAGGAAGTTTACTTTGGGAATGATGATGGTCAACTGATGAAGTATGCTGGTGTGTTGTATGGATTAGAAAATGTGGTTTGA

Protein sequence

>MS.gene034815.t1

MFSQNMFFAKPSTSSISIRPVWSFNLESEFKLIRSFVDSHPIISMDTEFPGVVILPDGITDLSSYHRTPAAHYSVLRANVDSLNIIQVGLTLSDVDGNLPKLRNGNSEESLIWEFNFSDFDFERDIHAHESIELLKSQGIDFEKNKEFGIDSRRFAELMMSSGLVCNEEVSWVTFHSGYDFGYLVKALTQYALPEELAEFLVLVRVFFGESVYDVKHLAKFCEGLYGGLDRVGKMLNVDRVVGKTHEAGSDSLLTLHAFRKIKEVYFGNDDGQLMKYAGVLYGLENVV