AlfalfaGEDB | MS.gene015616

M. sativa cultivar XinJiangDaYe / MS.gene015616

KEGG: K02960
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015616.t1	XP_003631001.1	100	140	0	0	1	140	1	140	2.40E-73	284.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015616.t1	P46293	94.3	140	8	0	1	140	6	145	1.2e-70	266.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015616.t1	G7LBJ0	100.0	140	0	0	1	140	1	140	1.7e-73	284.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene015616	MS.gene058560	PPI
MS.gene34284	MS.gene015616	PPI
MS.gene015616	MS.gene95856	PPI
MS.gene015616	MS.gene50088	PPI
MS.gene034319	MS.gene015616	PPI
MS.gene054733	MS.gene015616	PPI
MS.gene98113	MS.gene015616	PPI
MS.gene015616	MS.gene034319	PPI
MS.gene62652	MS.gene015616	PPI
MS.gene034318	MS.gene015616	PPI
MS.gene063979	MS.gene015616	PPI
MS.gene34285	MS.gene015616	PPI
MS.gene015616	MS.gene77759	PPI
MS.gene34283	MS.gene015616	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015616.t1	MTR_8g106080	100.000	140	0	1	140	1	140	1.17e-100	285
MS.gene015616.t1	MTR_8g106020	100.000	140	0	1	140	1	140	1.17e-100	285
MS.gene015616.t1	MTR_5g075240	99.286	140	1	1	140	1	140	2.63e-100	283
MS.gene015616.t1	MTR_4g085580	95.000	140	7	1	140	4	143	2.91e-96	273

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015616.t1	AT2G09990	89.928	139	14	2	140	8	146	5.16e-92	263
MS.gene015616.t1	AT5G18380	89.209	139	15	2	140	8	146	8.83e-92	262
MS.gene015616.t1	AT3G04230	84.173	139	22	2	140	8	146	1.84e-85	246
MS.gene015616.t1	AT5G18380	87.200	125	16	2	126	8	132	3.68e-79	230
MS.gene015616.t1	AT5G18380	87.037	108	14	2	109	8	115	1.14e-67	201

Find 40 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTAACGATAAGATTTC+TGG	0.210396	4.1:-1289328	None:intergenic
ACAGAGTAAGAAGGAGATTA+AGG	0.258952	4.1:+1289211	MS.gene015616:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	4.1:+1289281	MS.gene015616:CDS
CTGCAACAGCGTTCTTCTTC+CGG	0.375769	4.1:-1288947	None:intergenic
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	4.1:+1289160	MS.gene015616:CDS
GCCTGAGATTCTTCGTTTCA+AGG	0.392228	4.1:+1289034	MS.gene015616:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	4.1:-1289290	None:intergenic
TACGAGCCAATCCTCCTTCT+CGG	0.440837	4.1:+1289059	MS.gene015616:CDS
TCTGACGAATGGCATAGATC+TGG	0.445614	4.1:-1289142	None:intergenic
ATGGAACAGGTGCAATGCTT+TGG	0.453443	4.1:+1288924	MS.gene015616:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	4.1:-1289035	None:intergenic
GAAGCTCAGCAAGATGGAAC+AGG	0.488449	4.1:+1288911	None:intergenic
CGCGGTCTCATCAAGATCAA+TGG	0.494184	4.1:+1288990	MS.gene015616:CDS
TCATATCAACTCCGGCGAAA+CGG	0.494924	4.1:-1289088	None:intergenic
TCAGGCTCCACGAGTTCGAT+TGG	0.496656	4.1:-1289017	None:intergenic
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	4.1:+1289303	MS.gene015616:CDS
CTCGGAAGACACCGTTTCGC+CGG	0.504037	4.1:+1289077	MS.gene015616:CDS
GTTTCGCCGGAGTTGATATG+AGG	0.511168	4.1:+1289090	MS.gene015616:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	4.1:+1289290	MS.gene015616:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	4.1:+1289110	MS.gene015616:CDS
CTGACGAATGGCATAGATCT+GGG	0.534090	4.1:-1289141	None:intergenic
TGTTGATGAACAGAGTAAGA+AGG	0.534508	4.1:+1289202	MS.gene015616:CDS
AACAGGTGCAATGCTTTGGC+CGG	0.546867	4.1:+1288928	MS.gene015616:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	4.1:-1289070	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	4.1:+1289284	MS.gene015616:CDS
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	4.1:+1288972	MS.gene015616:CDS
TGATATGAGGATCCGTGTGA+AGG	0.569818	4.1:+1289103	MS.gene015616:CDS
GCAGTTACCCACTGCAAGAG+AGG	0.580125	4.1:+1288966	MS.gene015616:CDS
CATTCGTCAGAGTATTGCTA+AGG	0.581266	4.1:+1289154	MS.gene015616:CDS
TGTCTTCCGAGAAGGAGGAT+TGG	0.582501	4.1:-1289065	None:intergenic
TGGCTGTCCAATCGAACTCG+TGG	0.587642	4.1:+1289010	MS.gene015616:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	4.1:-1289275	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	4.1:+1289104	MS.gene015616:CDS
CTTAGCAATACTCTGACGAA+TGG	0.613778	4.1:-1289153	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	4.1:+1289107	MS.gene015616:CDS
ACCGCGACCTCTCTTGCAGT+GGG	0.618077	4.1:-1288973	None:intergenic
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	4.1:-1289073	None:intergenic
GACCGCGACCTCTCTTGCAG+TGG	0.624398	4.1:-1288974	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.629480	4.1:-1289115	None:intergenic
ACGGATCCTCATATCAACTC+CGG	0.648153	4.1:-1289096	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAGGAGATTA+AGG	+	chr4.1:1289211-1289230	MS.gene015616:CDS	35.0%
!	TGTTGATGAACAGAGTAAGA+AGG	+	chr4.1:1289202-1289221	MS.gene015616:CDS	35.0%
	CTTAGCAATACTCTGACGAA+TGG	-	chr4.1:1289156-1289175	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	+	chr4.1:1289154-1289173	MS.gene015616:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	+	chr4.1:1289160-1289179	MS.gene015616:CDS	40.0%
	ACGGATCCTCATATCAACTC+CGG	-	chr4.1:1289099-1289118	None:intergenic	45.0%
	ATGGAACAGGTGCAATGCTT+TGG	+	chr4.1:1288924-1288943	MS.gene015616:CDS	45.0%
	CTGACGAATGGCATAGATCT+GGG	-	chr4.1:1289144-1289163	None:intergenic	45.0%
	GATATGAGGATCCGTGTGAA+GGG	+	chr4.1:1289104-1289123	MS.gene015616:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	+	chr4.1:1289034-1289053	MS.gene015616:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	-	chr4.1:1289038-1289057	None:intergenic	45.0%
	TCATATCAACTCCGGCGAAA+CGG	-	chr4.1:1289091-1289110	None:intergenic	45.0%
	TCTGACGAATGGCATAGATC+TGG	-	chr4.1:1289145-1289164	None:intergenic	45.0%
	TGATATGAGGATCCGTGTGA+AGG	+	chr4.1:1289103-1289122	MS.gene015616:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	+	chr4.1:1289284-1289303	MS.gene015616:CDS	45.0%
	AACAGGTGCAATGCTTTGGC+CGG	+	chr4.1:1288928-1288947	MS.gene015616:CDS	50.0%
	CCACGACCACCAAACTTCTT+AGG	-	chr4.1:1289293-1289312	None:intergenic	50.0%
	CGCGGTCTCATCAAGATCAA+TGG	+	chr4.1:1288990-1289009	MS.gene015616:CDS	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	+	chr4.1:1289281-1289300	MS.gene015616:CDS	50.0%
	CTGCAACAGCGTTCTTCTTC+CGG	-	chr4.1:1288950-1288969	None:intergenic	50.0%
	TACGAGCCAATCCTCCTTCT+CGG	+	chr4.1:1289059-1289078	MS.gene015616:CDS	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	-	chr4.1:1289068-1289087	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	-	chr4.1:1289278-1289297	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	+	chr4.1:1289290-1289309	MS.gene015616:CDS	50.0%
!!	GTTTCGCCGGAGTTGATATG+AGG	+	chr4.1:1289090-1289109	MS.gene015616:CDS	50.0%
	AACGGTGTCTTCCGAGAAGG+AGG	-	chr4.1:1289073-1289092	None:intergenic	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	+	chr4.1:1289107-1289126	MS.gene015616:CDS	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	-	chr4.1:1289076-1289095	None:intergenic	55.0%
	GCAGTTACCCACTGCAAGAG+AGG	+	chr4.1:1288966-1288985	MS.gene015616:CDS	55.0%
	TCAGGCTCCACGAGTTCGAT+TGG	-	chr4.1:1289020-1289039	None:intergenic	55.0%
	TGGCTGTCCAATCGAACTCG+TGG	+	chr4.1:1289010-1289029	MS.gene015616:CDS	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	-	chr4.1:1289118-1289137	None:intergenic	55.0%
	ACCCACTGCAAGAGAGGTCG+CGG	+	chr4.1:1288972-1288991	MS.gene015616:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	-	chr4.1:1288976-1288995	None:intergenic	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	+	chr4.1:1289110-1289129	MS.gene015616:CDS	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	+	chr4.1:1289077-1289096	MS.gene015616:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	-	chr4.1:1288977-1288996	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	+	chr4.1:1289303-1289322	MS.gene015616:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	1288924	1289346	1288924	ID=MS.gene015616
chr4.1	mRNA	1288924	1289346	1288924	ID=MS.gene015616.t1;Parent=MS.gene015616
chr4.1	exon	1288924	1289346	1288924	ID=MS.gene015616.t1.exon1;Parent=MS.gene015616.t1
chr4.1	CDS	1288924	1289346	1288924	ID=cds.MS.gene015616.t1;Parent=MS.gene015616.t1

Gene Sequence

>MS.gene015616

ATGGAACAGGTGCAATGCTTTGGCCGGAAGAAGAACGCTGTTGCAGTTACCCACTGCAAGAGAGGTCGCGGTCTCATCAAGATCAATGGCTGTCCAATCGAACTCGTGGAGCCTGAGATTCTTCGTTTCAAGGCATACGAGCCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGAGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCCCAGATCTATGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTATTATCAGAAATATGTTGATGAACAGAGTAAGAAGGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>MS.gene015616.t1

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKYVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR