AlfalfaGEDB | MS.gene34284

M. sativa cultivar XinJiangDaYe / MS.gene34284

KEGG: K02960
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34284.t1	XP_003631001.1	99.3	139	1	0	1	139	1	139	1.20E-72	282.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34284.t1	P46293	93.5	139	9	0	1	139	6	144	6.2e-70	264.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34284.t1	G7LBJ0	99.3	139	1	0	1	139	1	139	8.5e-73	282.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050252	MS.gene34284	0.864011	1.65E-64	-1.69E-46
MS.gene050290	MS.gene34284	0.880165	6.87E-70	-1.69E-46
MS.gene051904	MS.gene34284	0.826214	3.07E-54	-1.69E-46
MS.gene054937	MS.gene34284	0.867626	1.19E-65	-1.69E-46
MS.gene057052	MS.gene34284	0.845817	3.20E-59	-1.69E-46
MS.gene057206	MS.gene34284	0.814937	1.19E-51	-1.69E-46
MS.gene057235	MS.gene34284	0.826148	3.18E-54	-1.69E-46
MS.gene060856	MS.gene34284	0.839356	1.66E-57	-1.69E-46
MS.gene061145	MS.gene34284	0.862809	3.90E-64	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene34284	MS.gene98113	PPI
MS.gene34284	MS.gene34283	PPI
MS.gene34284	MS.gene015616	PPI
MS.gene34284	MS.gene058560	PPI
MS.gene34284	MS.gene34285	PPI
MS.gene34284	MS.gene50088	PPI
MS.gene34284	MS.gene054733	PPI
MS.gene34284	MS.gene034319	PPI
MS.gene34284	MS.gene063979	PPI
MS.gene34284	MS.gene034318	PPI
MS.gene34284	MS.gene62652	PPI
MS.gene34284	MS.gene77759	PPI
MS.gene34284	MS.gene95856	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34284.t1	MTR_8g106080	99.281	139	1	1	139	1	139	1.65e-99	281
MS.gene34284.t1	MTR_8g106020	99.281	139	1	1	139	1	139	1.65e-99	281
MS.gene34284.t1	MTR_5g075240	98.561	139	2	1	139	1	139	3.41e-99	281
MS.gene34284.t1	MTR_4g085580	94.245	139	8	1	139	4	142	4.90e-95	270

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34284.t1	AT2G09990	89.130	138	15	2	139	8	145	8.59e-91	259
MS.gene34284.t1	AT5G18380	88.406	138	16	2	139	8	145	1.27e-90	259
MS.gene34284.t1	AT3G04230	83.333	138	23	2	139	8	145	2.77e-84	243
MS.gene34284.t1	AT5G18380	86.400	125	17	2	126	8	132	1.41e-78	229
MS.gene34284.t1	AT5G18380	86.111	108	15	2	109	8	115	6.31e-67	199

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTAACGATAAGATTTC+TGG	0.210396	4.1:+89803692	None:intergenic
ACAGAGTAAGAAAGAGATTA+AGG	0.239341	4.1:-89803809	MS.gene34284:CDS
CCGCAACGGCGTTCTTCTTC+CGG	0.308388	4.1:+89804073	None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG	0.344740	4.1:-89803986	MS.gene34284:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	4.1:-89803739	MS.gene34284:CDS
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	4.1:-89803860	MS.gene34284:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	4.1:+89803730	None:intergenic
TACGAACCAATCCTCCTTCT+CGG	0.444217	4.1:-89803961	MS.gene34284:CDS
GTTTCGCCGGCGTTGATATG+AGG	0.478501	4.1:-89803930	MS.gene34284:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	4.1:+89803985	None:intergenic
CTCGGAAGACACCGTTTCGC+CGG	0.484088	4.1:-89803943	MS.gene34284:CDS
ATGGAACAAGTGCAATGCTT+TGG	0.487256	4.1:-89804096	MS.gene34284:CDS
CAACGGCTGTCCAATCGAAC+TGG	0.488470	4.1:-89804013	MS.gene34284:CDS
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	4.1:-89803717	MS.gene34284:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	4.1:-89803730	MS.gene34284:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	4.1:-89803910	MS.gene34284:CDS
TCATATCAACGCCGGCGAAA+CGG	0.524265	4.1:+89803932	None:intergenic
TCAGGCTCAACCAGTTCGAT+TGG	0.524396	4.1:+89804003	None:intergenic
AACAAGTGCAATGCTTTGGC+CGG	0.546867	4.1:-89804092	MS.gene34284:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	4.1:+89803950	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	4.1:-89803736	MS.gene34284:CDS
GACCGCGACCTCTCTTGCAG+TGG	0.558828	4.1:+89804046	None:intergenic
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	4.1:-89804048	MS.gene34284:CDS
CATTCGTCAGAGTATTGCTA+AGG	0.576627	4.1:-89803866	MS.gene34284:CDS
TGATATGAGGATCCGTGTGA+AGG	0.579448	4.1:-89803917	MS.gene34284:CDS
GCGGTAACCCACTGCAAGAG+AGG	0.580471	4.1:-89804054	MS.gene34284:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	4.1:+89803745	None:intergenic
TGTCTTCCGAGAAGGAGGAT+TGG	0.592227	4.1:+89803955	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	4.1:-89803916	MS.gene34284:CDS
CTTGCAGTGGGTTACCGCAA+CGG	0.599991	4.1:+89804059	None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG	0.603590	4.1:+89804047	None:intergenic
CGCGGTCTGATCAAGATCAA+CGG	0.609302	4.1:-89804030	MS.gene34284:CDS
CTTAGCAATACTCTGACGAA+TGG	0.614188	4.1:+89803867	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	4.1:-89803913	MS.gene34284:CDS
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	4.1:+89803947	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.629480	4.1:+89803905	None:intergenic
CCGGAAGAAGAACGCCGTTG+CGG	0.651449	4.1:-89804073	MS.gene34284:CDS
ACGGATCCTCATATCAACGC+CGG	0.685813	4.1:+89803924	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAAGAGATTA+AGG	-	chr4.1:89803988-89804007	MS.gene34284:CDS	30.0%
	ATGGAACAAGTGCAATGCTT+TGG	-	chr4.1:89803701-89803720	MS.gene34284:CDS	40.0%
	CTTAGCAATACTCTGACGAA+TGG	+	chr4.1:89803933-89803952	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	-	chr4.1:89803931-89803950	MS.gene34284:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	-	chr4.1:89803937-89803956	MS.gene34284:CDS	40.0%
	AACAAGTGCAATGCTTTGGC+CGG	-	chr4.1:89803705-89803724	MS.gene34284:CDS	45.0%
	GATATGAGGATCCGTGTGAA+GGG	-	chr4.1:89803881-89803900	MS.gene34284:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	-	chr4.1:89803811-89803830	MS.gene34284:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	+	chr4.1:89803815-89803834	None:intergenic	45.0%
	TACGAACCAATCCTCCTTCT+CGG	-	chr4.1:89803836-89803855	MS.gene34284:CDS	45.0%
	TGATATGAGGATCCGTGTGA+AGG	-	chr4.1:89803880-89803899	MS.gene34284:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	-	chr4.1:89804061-89804080	MS.gene34284:CDS	45.0%
	ACGGATCCTCATATCAACGC+CGG	+	chr4.1:89803876-89803895	None:intergenic	50.0%
	CCACGACCACCAAACTTCTT+AGG	+	chr4.1:89804070-89804089	None:intergenic	50.0%
	CGCGGTCTGATCAAGATCAA+CGG	-	chr4.1:89803767-89803786	MS.gene34284:CDS	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	-	chr4.1:89804058-89804077	MS.gene34284:CDS	50.0%
	TCAGGCTCAACCAGTTCGAT+TGG	+	chr4.1:89803797-89803816	None:intergenic	50.0%
	TCATATCAACGCCGGCGAAA+CGG	+	chr4.1:89803868-89803887	None:intergenic	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	+	chr4.1:89803845-89803864	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	+	chr4.1:89804055-89804074	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	-	chr4.1:89804067-89804086	MS.gene34284:CDS	50.0%
	AACGGTGTCTTCCGAGAAGG+AGG	+	chr4.1:89803850-89803869	None:intergenic	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	-	chr4.1:89803884-89803903	MS.gene34284:CDS	55.0%
	CAACGGCTGTCCAATCGAAC+TGG	-	chr4.1:89803784-89803803	MS.gene34284:CDS	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	+	chr4.1:89803853-89803872	None:intergenic	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	+	chr4.1:89803895-89803914	None:intergenic	55.0%
!!	CTTGCAGTGGGTTACCGCAA+CGG	+	chr4.1:89803741-89803760	None:intergenic	55.0%
!!	GTTTCGCCGGCGTTGATATG+AGG	-	chr4.1:89803867-89803886	MS.gene34284:CDS	55.0%
	ACCCACTGCAAGAGAGGTCG+CGG	-	chr4.1:89803749-89803768	MS.gene34284:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	+	chr4.1:89803753-89803772	None:intergenic	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	-	chr4.1:89803887-89803906	MS.gene34284:CDS	60.0%
	CCGCAACGGCGTTCTTCTTC+CGG	+	chr4.1:89803727-89803746	None:intergenic	60.0%
	CCGGAAGAAGAACGCCGTTG+CGG	-	chr4.1:89803724-89803743	MS.gene34284:CDS	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	-	chr4.1:89803854-89803873	MS.gene34284:CDS	60.0%
	GCGGTAACCCACTGCAAGAG+AGG	-	chr4.1:89803743-89803762	MS.gene34284:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	+	chr4.1:89803754-89803773	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	-	chr4.1:89804080-89804099	MS.gene34284:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	89803701	89804118	89803701	ID=MS.gene34284
chr4.1	mRNA	89803701	89804118	89803701	ID=MS.gene34284.t1;Parent=MS.gene34284
chr4.1	exon	89803701	89804118	89803701	ID=MS.gene34284.t1.exon1;Parent=MS.gene34284.t1
chr4.1	CDS	89803701	89804118	89803701	ID=cds.MS.gene34284.t1;Parent=MS.gene34284.t1

Gene Sequence

>MS.gene34284

ATGGAACAAGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCGGTAACCCACTGCAAGAGAGGTCGCGGTCTGATCAAGATCAACGGCTGTCCAATCGAACTGGTTGAGCCTGAGATTCTTCGTTTCAAGGCATACGAACCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGCGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCTCAGATCTACGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTACTATCAGAAATTTGTTGATGAACAGAGTAAGAAAGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATC

Protein sequence

>MS.gene34284.t1

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKFVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSY