AlfalfaGEDB | MS.gene50088

M. sativa cultivar XinJiangDaYe / MS.gene50088

KEGG: K02960
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50088.t1	XP_003631001.1	98.6	140	2	0	1	140	1	140	2.70E-72	281.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50088.t1	P46293	92.9	140	10	0	1	140	6	145	1.4e-69	263.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50088.t1	G7LBJ0	98.6	140	2	0	1	140	1	140	1.9e-72	281.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050752	MS.gene50088	0.801589	8.38E-49	-1.69E-46
MS.gene050955	MS.gene50088	0.828049	1.12E-54	-1.69E-46
MS.gene051241	MS.gene50088	0.822491	2.30E-53	-1.69E-46
MS.gene051903	MS.gene50088	0.818512	1.89E-52	-1.69E-46
MS.gene052947	MS.gene50088	0.834088	3.67E-56	-1.69E-46
MS.gene054403	MS.gene50088	0.802715	4.92E-49	-1.69E-46
MS.gene054937	MS.gene50088	0.805207	1.49E-49	-1.69E-46
MS.gene057705	MS.gene50088	0.80074	1.25E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene34283	MS.gene50088	PPI
MS.gene015616	MS.gene50088	PPI
MS.gene34284	MS.gene50088	PPI
MS.gene034319	MS.gene50088	PPI
MS.gene054733	MS.gene50088	PPI
MS.gene50088	MS.gene95856	PPI
MS.gene98113	MS.gene50088	PPI
MS.gene50088	MS.gene77759	PPI
MS.gene62652	MS.gene50088	PPI
MS.gene50088	MS.gene058560	PPI
MS.gene034318	MS.gene50088	PPI
MS.gene063979	MS.gene50088	PPI
MS.gene34285	MS.gene50088	PPI
MS.gene95856	MS.gene50088	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50088.t1	MTR_8g106080	98.571	140	2	1	140	1	140	2.88e-99	281
MS.gene50088.t1	MTR_8g106020	98.571	140	2	1	140	1	140	2.88e-99	281
MS.gene50088.t1	MTR_5g075240	97.857	140	3	1	140	1	140	7.01e-99	280
MS.gene50088.t1	MTR_4g085580	93.571	140	9	1	140	4	143	1.12e-94	270

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50088.t1	AT2G09990	87.857	140	17	1	140	7	146	1.69e-90	259
MS.gene50088.t1	AT5G18380	87.143	140	18	1	140	7	146	2.35e-90	259
MS.gene50088.t1	AT3G04230	82.734	139	24	2	140	8	146	4.14e-84	243
MS.gene50088.t1	AT5G18380	85.600	125	18	2	126	8	132	1.34e-77	226
MS.gene50088.t1	AT5G18380	85.185	108	16	2	109	8	115	5.27e-66	197

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTAACGATAAGATTTC+TGG	0.210396	4.3:-90144324	None:intergenic
ACAGAGTAAGAAAGAGATTA+AGG	0.239341	4.3:+90144207	MS.gene50088:CDS
CCGCAACGGCGTTCTTCTTC+CGG	0.308388	4.3:-90143943	None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG	0.344740	4.3:+90144030	MS.gene50088:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	4.3:+90144277	MS.gene50088:CDS
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	4.3:+90144156	MS.gene50088:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	4.3:-90144286	None:intergenic
TACGAACCAATCCTCCTTCT+CGG	0.444217	4.3:+90144055	MS.gene50088:CDS
GTTTCGCCGGCGTTGATATG+AGG	0.478501	4.3:+90144086	MS.gene50088:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	4.3:-90144031	None:intergenic
CTCGGAAGACACCGTTTCGC+CGG	0.484088	4.3:+90144073	MS.gene50088:CDS
ATGGAACAAGTGCAATGCTT+TGG	0.487256	4.3:+90143920	MS.gene50088:CDS
CAACGGCTGTCCAATCGAAC+TGG	0.488470	4.3:+90144003	MS.gene50088:CDS
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	4.3:+90144299	MS.gene50088:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	4.3:+90144286	MS.gene50088:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	4.3:+90144106	MS.gene50088:CDS
TCATATCAACGCCGGCGAAA+CGG	0.524265	4.3:-90144084	None:intergenic
TCAGGCTCAACCAGTTCGAT+TGG	0.524396	4.3:-90144013	None:intergenic
AACAAGTGCAATGCTTTGGC+CGG	0.546867	4.3:+90143924	MS.gene50088:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	4.3:-90144066	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	4.3:+90144280	MS.gene50088:CDS
GACCGCGACCTCTCTTGCAG+TGG	0.558828	4.3:-90143970	None:intergenic
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	4.3:+90143968	MS.gene50088:CDS
CATTCGTCAGAGTATTGCTA+AGG	0.576627	4.3:+90144150	MS.gene50088:CDS
TGATATGAGGATCCGTGTGA+AGG	0.579448	4.3:+90144099	MS.gene50088:CDS
GCGGTAACCCACTGCAAGAG+AGG	0.580471	4.3:+90143962	MS.gene50088:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	4.3:-90144271	None:intergenic
TGTCTTCCGAGAAGGAGGAT+TGG	0.592227	4.3:-90144061	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	4.3:+90144100	MS.gene50088:CDS
CTTGCAGTGGGTTACCGCAA+CGG	0.599991	4.3:-90143957	None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG	0.603590	4.3:-90143969	None:intergenic
CGCGGTCTGATCAAGATCAA+CGG	0.609302	4.3:+90143986	MS.gene50088:CDS
CTTAGCAATACTCTGACGAA+TGG	0.614188	4.3:-90144149	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	4.3:+90144103	MS.gene50088:CDS
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	4.3:-90144069	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.624846	4.3:-90144111	None:intergenic
CCGGAAGAAGAACGCCGTTG+CGG	0.651449	4.3:+90143943	MS.gene50088:CDS
ACGGATCCTCATATCAACGC+CGG	0.685813	4.3:-90144092	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAAGAGATTA+AGG	+	chr4.3:90144207-90144226	MS.gene50088:CDS	30.0%
	ATGGAACAAGTGCAATGCTT+TGG	+	chr4.3:90143920-90143939	MS.gene50088:CDS	40.0%
	CTTAGCAATACTCTGACGAA+TGG	-	chr4.3:90144152-90144171	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	+	chr4.3:90144150-90144169	MS.gene50088:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	+	chr4.3:90144156-90144175	MS.gene50088:CDS	40.0%
	AACAAGTGCAATGCTTTGGC+CGG	+	chr4.3:90143924-90143943	MS.gene50088:CDS	45.0%
	GATATGAGGATCCGTGTGAA+GGG	+	chr4.3:90144100-90144119	MS.gene50088:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	+	chr4.3:90144030-90144049	MS.gene50088:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	-	chr4.3:90144034-90144053	None:intergenic	45.0%
	TACGAACCAATCCTCCTTCT+CGG	+	chr4.3:90144055-90144074	MS.gene50088:CDS	45.0%
	TGATATGAGGATCCGTGTGA+AGG	+	chr4.3:90144099-90144118	MS.gene50088:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	+	chr4.3:90144280-90144299	MS.gene50088:CDS	45.0%
	ACGGATCCTCATATCAACGC+CGG	-	chr4.3:90144095-90144114	None:intergenic	50.0%
	CCACGACCACCAAACTTCTT+AGG	-	chr4.3:90144289-90144308	None:intergenic	50.0%
	CGCGGTCTGATCAAGATCAA+CGG	+	chr4.3:90143986-90144005	MS.gene50088:CDS	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	+	chr4.3:90144277-90144296	MS.gene50088:CDS	50.0%
	TCAGGCTCAACCAGTTCGAT+TGG	-	chr4.3:90144016-90144035	None:intergenic	50.0%
	TCATATCAACGCCGGCGAAA+CGG	-	chr4.3:90144087-90144106	None:intergenic	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	-	chr4.3:90144064-90144083	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	-	chr4.3:90144274-90144293	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	+	chr4.3:90144286-90144305	MS.gene50088:CDS	50.0%
	AACGGTGTCTTCCGAGAAGG+AGG	-	chr4.3:90144069-90144088	None:intergenic	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	+	chr4.3:90144103-90144122	MS.gene50088:CDS	55.0%
	CAACGGCTGTCCAATCGAAC+TGG	+	chr4.3:90144003-90144022	MS.gene50088:CDS	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	-	chr4.3:90144072-90144091	None:intergenic	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	-	chr4.3:90144114-90144133	None:intergenic	55.0%
!!	CTTGCAGTGGGTTACCGCAA+CGG	-	chr4.3:90143960-90143979	None:intergenic	55.0%
!!	GTTTCGCCGGCGTTGATATG+AGG	+	chr4.3:90144086-90144105	MS.gene50088:CDS	55.0%
	ACCCACTGCAAGAGAGGTCG+CGG	+	chr4.3:90143968-90143987	MS.gene50088:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	-	chr4.3:90143972-90143991	None:intergenic	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	+	chr4.3:90144106-90144125	MS.gene50088:CDS	60.0%
	CCGCAACGGCGTTCTTCTTC+CGG	-	chr4.3:90143946-90143965	None:intergenic	60.0%
	CCGGAAGAAGAACGCCGTTG+CGG	+	chr4.3:90143943-90143962	MS.gene50088:CDS	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	+	chr4.3:90144073-90144092	MS.gene50088:CDS	60.0%
	GCGGTAACCCACTGCAAGAG+AGG	+	chr4.3:90143962-90143981	MS.gene50088:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	-	chr4.3:90143973-90143992	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	+	chr4.3:90144299-90144318	MS.gene50088:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	90143920	90144342	90143920	ID=MS.gene50088
chr4.3	mRNA	90143920	90144342	90143920	ID=MS.gene50088.t1;Parent=MS.gene50088
chr4.3	exon	90143920	90144342	90143920	ID=MS.gene50088.t1.exon1;Parent=MS.gene50088.t1
chr4.3	CDS	90143920	90144342	90143920	ID=cds.MS.gene50088.t1;Parent=MS.gene50088.t1

Gene Sequence

>MS.gene50088

ATGGAACAAGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCGGTAACCCACTGCAAGAGAGGTCGCGGTCTGATCAAGATCAACGGCTGTCCAATCGAACTGGTTGAGCCTGAGATTCTTCGTTTCAAGGCATACGAACCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGCGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACATTTCTCAGATCTACGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTACTATCAGAAATTTGTTGATGAACAGAGTAAGAAAGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>MS.gene50088.t1

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHISQIYAIRQSIAKALVAYYQKFVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR