AlfalfaGEDB | MS.gene98113

M. sativa cultivar XinJiangDaYe / MS.gene98113

KEGG: K02960
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98113.t1	XP_003631001.1	99.3	140	1	0	1	140	1	140	5.40E-73	283.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98113.t1	P46293	93.6	140	9	0	1	140	6	145	2.8e-70	265.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98113.t1	G7LBJ0	99.3	140	1	0	1	140	1	140	3.9e-73	283.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050252	MS.gene98113	0.869631	2.68E-66	-1.69E-46
MS.gene050290	MS.gene98113	0.862845	3.80E-64	-1.69E-46
MS.gene051904	MS.gene98113	0.831386	1.72E-55	-1.69E-46
MS.gene054733	MS.gene98113	0.805865	1.09E-49	-1.69E-46
MS.gene054937	MS.gene98113	0.842654	2.27E-58	-1.69E-46
MS.gene057052	MS.gene98113	0.834456	2.97E-56	-1.69E-46
MS.gene057235	MS.gene98113	0.822352	2.48E-53	-1.69E-46
MS.gene060856	MS.gene98113	0.825322	4.99E-54	-1.69E-46
MS.gene061145	MS.gene98113	0.841621	4.25E-58	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene34284	MS.gene98113	PPI
MS.gene98113	MS.gene054733	PPI
MS.gene98113	MS.gene62652	PPI
MS.gene98113	MS.gene77759	PPI
MS.gene98113	MS.gene015616	PPI
MS.gene98113	MS.gene95856	PPI
MS.gene98113	MS.gene50088	PPI
MS.gene034318	MS.gene98113	PPI
MS.gene063979	MS.gene98113	PPI
MS.gene34285	MS.gene98113	PPI
MS.gene98113	MS.gene058560	PPI
MS.gene34283	MS.gene98113	PPI
MS.gene98113	MS.gene034319	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98113.t1	MTR_8g106080	99.286	140	1	1	140	1	140	3.04e-100	283
MS.gene98113.t1	MTR_8g106020	99.286	140	1	1	140	1	140	3.04e-100	283
MS.gene98113.t1	MTR_5g075240	98.571	140	2	1	140	1	140	7.72e-100	282
MS.gene98113.t1	MTR_4g085580	94.286	140	8	1	140	4	143	9.84e-96	272

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98113.t1	AT2G09990	89.209	139	15	2	140	8	146	1.84e-91	261
MS.gene98113.t1	AT5G18380	88.489	139	16	2	140	8	146	3.12e-91	261
MS.gene98113.t1	AT3G04230	83.453	139	23	2	140	8	146	5.94e-85	245
MS.gene98113.t1	AT5G18380	86.400	125	17	2	126	8	132	1.60e-78	229
MS.gene98113.t1	AT5G18380	86.111	108	15	2	109	8	115	5.33e-67	199

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAGAGTAAGAAAGAGATTA+AGG	0.239341	4.1:+89715443	MS.gene98113:CDS
AAATCTAACGATAAGATTTC+TGG	0.303017	4.1:-89715560	None:intergenic
CCGCAACGGCGTTCTTCTTC+CGG	0.308388	4.1:-89715179	None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG	0.344740	4.1:+89715266	MS.gene98113:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	4.1:+89715513	MS.gene98113:CDS
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	4.1:+89715392	MS.gene98113:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	4.1:-89715522	None:intergenic
TACGAACCAATCCTCCTTCT+CGG	0.444217	4.1:+89715291	MS.gene98113:CDS
GTTTCGCCGGCGTTGATATG+AGG	0.478501	4.1:+89715322	MS.gene98113:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	4.1:-89715267	None:intergenic
CTCGGAAGACACCGTTTCGC+CGG	0.484088	4.1:+89715309	MS.gene98113:CDS
ATGGAACAAGTGCAATGCTT+TGG	0.487256	4.1:+89715156	MS.gene98113:CDS
CAACGGCTGTCCAATCGAAC+TGG	0.488470	4.1:+89715239	MS.gene98113:CDS
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	4.1:+89715535	MS.gene98113:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	4.1:+89715522	MS.gene98113:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	4.1:+89715342	MS.gene98113:CDS
TCATATCAACGCCGGCGAAA+CGG	0.524265	4.1:-89715320	None:intergenic
TCAGGCTCAACCAGTTCGAT+TGG	0.524396	4.1:-89715249	None:intergenic
AACAAGTGCAATGCTTTGGC+CGG	0.546867	4.1:+89715160	MS.gene98113:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	4.1:-89715302	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	4.1:+89715516	MS.gene98113:CDS
GACCGCGACCTCTCTTGCAG+TGG	0.558828	4.1:-89715206	None:intergenic
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	4.1:+89715204	MS.gene98113:CDS
CATTCGTCAGAGTATTGCTA+AGG	0.576627	4.1:+89715386	MS.gene98113:CDS
TGATATGAGGATCCGTGTGA+AGG	0.579448	4.1:+89715335	MS.gene98113:CDS
GCGGTAACCCACTGCAAGAG+AGG	0.580471	4.1:+89715198	MS.gene98113:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	4.1:-89715507	None:intergenic
TGTCTTCCGAGAAGGAGGAT+TGG	0.592227	4.1:-89715297	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	4.1:+89715336	MS.gene98113:CDS
CTTGCAGTGGGTTACCGCAA+CGG	0.599991	4.1:-89715193	None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG	0.603590	4.1:-89715205	None:intergenic
CGCGGTCTGATCAAGATCAA+CGG	0.609302	4.1:+89715222	MS.gene98113:CDS
CTTAGCAATACTCTGACGAA+TGG	0.614188	4.1:-89715385	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	4.1:+89715339	MS.gene98113:CDS
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	4.1:-89715305	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.629480	4.1:-89715347	None:intergenic
CCGGAAGAAGAACGCCGTTG+CGG	0.651449	4.1:+89715179	MS.gene98113:CDS
ACGGATCCTCATATCAACGC+CGG	0.685813	4.1:-89715328	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAAGAGATTA+AGG	+	chr4.1:89715443-89715462	MS.gene98113:CDS	30.0%
	ATGGAACAAGTGCAATGCTT+TGG	+	chr4.1:89715156-89715175	MS.gene98113:CDS	40.0%
	CTTAGCAATACTCTGACGAA+TGG	-	chr4.1:89715388-89715407	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	+	chr4.1:89715386-89715405	MS.gene98113:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	+	chr4.1:89715392-89715411	MS.gene98113:CDS	40.0%
	AACAAGTGCAATGCTTTGGC+CGG	+	chr4.1:89715160-89715179	MS.gene98113:CDS	45.0%
	GATATGAGGATCCGTGTGAA+GGG	+	chr4.1:89715336-89715355	MS.gene98113:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	+	chr4.1:89715266-89715285	MS.gene98113:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	-	chr4.1:89715270-89715289	None:intergenic	45.0%
	TACGAACCAATCCTCCTTCT+CGG	+	chr4.1:89715291-89715310	MS.gene98113:CDS	45.0%
	TGATATGAGGATCCGTGTGA+AGG	+	chr4.1:89715335-89715354	MS.gene98113:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	+	chr4.1:89715516-89715535	MS.gene98113:CDS	45.0%
	ACGGATCCTCATATCAACGC+CGG	-	chr4.1:89715331-89715350	None:intergenic	50.0%
	CCACGACCACCAAACTTCTT+AGG	-	chr4.1:89715525-89715544	None:intergenic	50.0%
	CGCGGTCTGATCAAGATCAA+CGG	+	chr4.1:89715222-89715241	MS.gene98113:CDS	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	+	chr4.1:89715513-89715532	MS.gene98113:CDS	50.0%
	TCAGGCTCAACCAGTTCGAT+TGG	-	chr4.1:89715252-89715271	None:intergenic	50.0%
	TCATATCAACGCCGGCGAAA+CGG	-	chr4.1:89715323-89715342	None:intergenic	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	-	chr4.1:89715300-89715319	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	-	chr4.1:89715510-89715529	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	+	chr4.1:89715522-89715541	MS.gene98113:CDS	50.0%
	AACGGTGTCTTCCGAGAAGG+AGG	-	chr4.1:89715305-89715324	None:intergenic	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	+	chr4.1:89715339-89715358	MS.gene98113:CDS	55.0%
	CAACGGCTGTCCAATCGAAC+TGG	+	chr4.1:89715239-89715258	MS.gene98113:CDS	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	-	chr4.1:89715308-89715327	None:intergenic	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	-	chr4.1:89715350-89715369	None:intergenic	55.0%
!!	CTTGCAGTGGGTTACCGCAA+CGG	-	chr4.1:89715196-89715215	None:intergenic	55.0%
!!	GTTTCGCCGGCGTTGATATG+AGG	+	chr4.1:89715322-89715341	MS.gene98113:CDS	55.0%
	ACCCACTGCAAGAGAGGTCG+CGG	+	chr4.1:89715204-89715223	MS.gene98113:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	-	chr4.1:89715208-89715227	None:intergenic	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	+	chr4.1:89715342-89715361	MS.gene98113:CDS	60.0%
	CCGCAACGGCGTTCTTCTTC+CGG	-	chr4.1:89715182-89715201	None:intergenic	60.0%
	CCGGAAGAAGAACGCCGTTG+CGG	+	chr4.1:89715179-89715198	MS.gene98113:CDS	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	+	chr4.1:89715309-89715328	MS.gene98113:CDS	60.0%
	GCGGTAACCCACTGCAAGAG+AGG	+	chr4.1:89715198-89715217	MS.gene98113:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	-	chr4.1:89715209-89715228	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	+	chr4.1:89715535-89715554	MS.gene98113:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	89715156	89715578	89715156	ID=MS.gene98113
chr4.1	mRNA	89715156	89715578	89715156	ID=MS.gene98113.t1;Parent=MS.gene98113
chr4.1	exon	89715156	89715578	89715156	ID=MS.gene98113.t1.exon1;Parent=MS.gene98113.t1
chr4.1	CDS	89715156	89715578	89715156	ID=cds.MS.gene98113.t1;Parent=MS.gene98113.t1

Gene Sequence

>MS.gene98113

ATGGAACAAGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCGGTAACCCACTGCAAGAGAGGTCGCGGTCTGATCAAGATCAACGGCTGTCCAATCGAACTGGTTGAGCCTGAGATTCTTCGTTTCAAGGCATACGAACCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGCGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCTCAGATCTACGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTACTATCAGAAATTTGTTGATGAACAGAGTAAGAAAGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>MS.gene98113.t1

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKFVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR