AlfalfaGEDB | MS.gene054733

M. sativa cultivar XinJiangDaYe / MS.gene054733

KEGG: K02960
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054733.t1	XP_003631001.1	100	140	0	0	1	140	1	140	2.40E-73	284.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054733.t1	P46293	94.3	140	8	0	1	140	6	145	1.2e-70	266.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054733.t1	G7LBJ0	100.0	140	0	0	1	140	1	140	1.7e-73	284.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050252	MS.gene054733	0.819362	1.21E-52	-1.69E-46
MS.gene054733	MS.gene058994	0.834653	2.65E-56	-1.69E-46
MS.gene054733	MS.gene063979	0.97454	2.46E-138	-1.69E-46
MS.gene054733	MS.gene073021	0.807233	5.59E-50	-1.69E-46
MS.gene054733	MS.gene20295	0.824697	7.01E-54	-1.69E-46
MS.gene054733	MS.gene38222	0.801648	8.15E-49	-1.69E-46
MS.gene054733	MS.gene42419	0.800502	1.40E-48	-1.69E-46
MS.gene054733	MS.gene48048	0.84432	8.13E-59	-1.69E-46
MS.gene054733	MS.gene57391	0.805182	1.51E-49	-1.69E-46
MS.gene054733	MS.gene70215	0.805803	1.12E-49	-1.69E-46
MS.gene054733	MS.gene93753	0.800156	1.65E-48	-1.69E-46
MS.gene054733	MS.gene95856	0.858086	1.05E-62	-1.69E-46
MS.gene054733	MS.gene98113	0.805865	1.09E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene034318	MS.gene054733	PPI
MS.gene98113	MS.gene054733	PPI
MS.gene063979	MS.gene054733	PPI
MS.gene34285	MS.gene054733	PPI
MS.gene054733	MS.gene62652	PPI
MS.gene054733	MS.gene77759	PPI
MS.gene34283	MS.gene054733	PPI
MS.gene34284	MS.gene054733	PPI
MS.gene054733	MS.gene015616	PPI
MS.gene054733	MS.gene95856	PPI
MS.gene054733	MS.gene50088	PPI
MS.gene054733	MS.gene058560	PPI
MS.gene054733	MS.gene034319	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054733.t1	MTR_8g106080	100.000	140	0	1	140	1	140	1.17e-100	285
MS.gene054733.t1	MTR_8g106020	100.000	140	0	1	140	1	140	1.17e-100	285
MS.gene054733.t1	MTR_5g075240	99.286	140	1	1	140	1	140	2.63e-100	283
MS.gene054733.t1	MTR_4g085580	95.000	140	7	1	140	4	143	2.91e-96	273

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054733.t1	AT2G09990	89.928	139	14	2	140	8	146	5.16e-92	263
MS.gene054733.t1	AT5G18380	89.209	139	15	2	140	8	146	8.83e-92	262
MS.gene054733.t1	AT3G04230	84.173	139	22	2	140	8	146	1.84e-85	246
MS.gene054733.t1	AT5G18380	87.200	125	16	2	126	8	132	3.68e-79	230
MS.gene054733.t1	AT5G18380	87.037	108	14	2	109	8	115	1.14e-67	201

Find 39 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTCTAACGATAAGATTTC+TGG	0.233791	5.3:+33183745	None:intergenic
ACAGAGTAAGAAGGAGATTA+AGG	0.258952	5.3:-33183862	MS.gene054733:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	5.3:-33183792	MS.gene054733:CDS
CTGCAACGGCGTTCTTCTTC+CGG	0.366311	5.3:+33184126	None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG	0.392228	5.3:-33184039	MS.gene054733:CDS
TCAGAGTATTGCGAAGGCTT+TGG	0.400647	5.3:-33183913	MS.gene054733:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	5.3:+33183783	None:intergenic
TACGAACCAATCCTCCTTCT+CGG	0.444217	5.3:-33184014	MS.gene054733:CDS
ATGGAACAGGTGCAATGCTT+TGG	0.453443	5.3:-33184149	MS.gene054733:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	5.3:+33184038	None:intergenic
GAAGCTCAGCAAGATGGAAC+AGG	0.488449	5.3:-33184162	None:intergenic
CGCGGTCTCATCAAGATCAA+TGG	0.494184	5.3:-33184083	MS.gene054733:CDS
TCATATCAACTCCGGCGAAA+CGG	0.494924	5.3:+33183985	None:intergenic
TCAGGCTCCACGAGTTCGAT+TGG	0.496656	5.3:+33184056	None:intergenic
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	5.3:-33183770	MS.gene054733:CDS
CTCGGAAGACACCGTTTCGC+CGG	0.504037	5.3:-33183996	MS.gene054733:CDS
GTTTCGCCGGAGTTGATATG+AGG	0.511168	5.3:-33183983	MS.gene054733:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	5.3:-33183783	MS.gene054733:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	5.3:-33183963	MS.gene054733:CDS
TGTTGATGAACAGAGTAAGA+AGG	0.534508	5.3:-33183871	MS.gene054733:CDS
AACAGGTGCAATGCTTTGGC+CGG	0.546867	5.3:-33184145	MS.gene054733:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	5.3:+33184003	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	5.3:-33183789	MS.gene054733:CDS
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	5.3:-33184101	MS.gene054733:CDS
TGATATGAGGATCCGTGTGA+AGG	0.569818	5.3:-33183970	MS.gene054733:CDS
GCAGTTACCCACTGCAAGAG+AGG	0.582565	5.3:-33184107	MS.gene054733:CDS
TGGCTGTCCAATCGAACTCG+TGG	0.587642	5.3:-33184063	MS.gene054733:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	5.3:+33183798	None:intergenic
TGTCTTCCGAGAAGGAGGAT+TGG	0.592227	5.3:+33184008	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	5.3:-33183969	MS.gene054733:CDS
CTTCGCAATACTCTGACGAA+TGG	0.610127	5.3:+33183920	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	5.3:-33183966	MS.gene054733:CDS
ACCGCGACCTCTCTTGCAGT+GGG	0.618077	5.3:+33184100	None:intergenic
CATTCGTCAGAGTATTGCGA+AGG	0.620290	5.3:-33183919	MS.gene054733:CDS
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	5.3:+33184000	None:intergenic
GACCGCGACCTCTCTTGCAG+TGG	0.624398	5.3:+33184099	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.629480	5.3:+33183958	None:intergenic
CTTGCAGTGGGTAACTGCAA+CGG	0.644084	5.3:+33184112	None:intergenic
ACGGATCCTCATATCAACTC+CGG	0.648153	5.3:+33183977	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAGGAGATTA+AGG	-	chr5.3:33184036-33184055	MS.gene054733:CDS	35.0%
!	TGTTGATGAACAGAGTAAGA+AGG	-	chr5.3:33184027-33184046	MS.gene054733:CDS	35.0%
	ACGGATCCTCATATCAACTC+CGG	+	chr5.3:33183924-33183943	None:intergenic	45.0%
	ATGGAACAGGTGCAATGCTT+TGG	-	chr5.3:33183749-33183768	MS.gene054733:CDS	45.0%
	CTTCGCAATACTCTGACGAA+TGG	+	chr5.3:33183981-33184000	None:intergenic	45.0%
	GATATGAGGATCCGTGTGAA+GGG	-	chr5.3:33183929-33183948	MS.gene054733:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	-	chr5.3:33183859-33183878	MS.gene054733:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	+	chr5.3:33183863-33183882	None:intergenic	45.0%
	TACGAACCAATCCTCCTTCT+CGG	-	chr5.3:33183884-33183903	MS.gene054733:CDS	45.0%
	TCATATCAACTCCGGCGAAA+CGG	+	chr5.3:33183916-33183935	None:intergenic	45.0%
	TGATATGAGGATCCGTGTGA+AGG	-	chr5.3:33183928-33183947	MS.gene054733:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	-	chr5.3:33184109-33184128	MS.gene054733:CDS	45.0%
!!	CATTCGTCAGAGTATTGCGA+AGG	-	chr5.3:33183979-33183998	MS.gene054733:CDS	45.0%
!!	TCAGAGTATTGCGAAGGCTT+TGG	-	chr5.3:33183985-33184004	MS.gene054733:CDS	45.0%
	AACAGGTGCAATGCTTTGGC+CGG	-	chr5.3:33183753-33183772	MS.gene054733:CDS	50.0%
	CCACGACCACCAAACTTCTT+AGG	+	chr5.3:33184118-33184137	None:intergenic	50.0%
	CGCGGTCTCATCAAGATCAA+TGG	-	chr5.3:33183815-33183834	MS.gene054733:CDS	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	-	chr5.3:33184106-33184125	MS.gene054733:CDS	50.0%
	CTTGCAGTGGGTAACTGCAA+CGG	+	chr5.3:33183789-33183808	None:intergenic	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	+	chr5.3:33183893-33183912	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	+	chr5.3:33184103-33184122	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	-	chr5.3:33184115-33184134	MS.gene054733:CDS	50.0%
!!	GTTTCGCCGGAGTTGATATG+AGG	-	chr5.3:33183915-33183934	MS.gene054733:CDS	50.0%
	AACGGTGTCTTCCGAGAAGG+AGG	+	chr5.3:33183898-33183917	None:intergenic	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	-	chr5.3:33183932-33183951	MS.gene054733:CDS	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	+	chr5.3:33183901-33183920	None:intergenic	55.0%
	CTGCAACGGCGTTCTTCTTC+CGG	+	chr5.3:33183775-33183794	None:intergenic	55.0%
	GCAGTTACCCACTGCAAGAG+AGG	-	chr5.3:33183791-33183810	MS.gene054733:CDS	55.0%
	TCAGGCTCCACGAGTTCGAT+TGG	+	chr5.3:33183845-33183864	None:intergenic	55.0%
	TGGCTGTCCAATCGAACTCG+TGG	-	chr5.3:33183835-33183854	MS.gene054733:CDS	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	+	chr5.3:33183943-33183962	None:intergenic	55.0%
	ACCCACTGCAAGAGAGGTCG+CGG	-	chr5.3:33183797-33183816	MS.gene054733:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	+	chr5.3:33183801-33183820	None:intergenic	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	-	chr5.3:33183935-33183954	MS.gene054733:CDS	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	-	chr5.3:33183902-33183921	MS.gene054733:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	+	chr5.3:33183802-33183821	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	-	chr5.3:33184128-33184147	MS.gene054733:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	33183749	33184171	33183749	ID=MS.gene054733
chr5.3	mRNA	33183749	33184171	33183749	ID=MS.gene054733.t1;Parent=MS.gene054733
chr5.3	exon	33183749	33184171	33183749	ID=MS.gene054733.t1.exon1;Parent=MS.gene054733.t1
chr5.3	CDS	33183749	33184171	33183749	ID=cds.MS.gene054733.t1;Parent=MS.gene054733.t1

Gene Sequence

>MS.gene054733

ATGGAACAGGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCAGTTACCCACTGCAAGAGAGGTCGCGGTCTCATCAAGATCAATGGCTGTCCAATCGAACTCGTGGAGCCTGAGATTCTTCGTTTCAAGGCATACGAACCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGAGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCTCAGATATATGCCATTCGTCAGAGTATTGCGAAGGCTTTGGTTGCTTATTATCAGAAATATGTTGATGAACAGAGTAAGAAGGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>MS.gene054733.t1

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKYVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR