AlfalfaGEDB | MS.gene95856

M. sativa cultivar XinJiangDaYe / MS.gene95856

KEGG: K02960
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95856.t1	XP_003631001.1	100	140	0	0	1	140	1	140	2.40E-73	284.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95856.t1	P46293	94.3	140	8	0	1	140	6	145	1.2e-70	266.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95856.t1	G7LBJ0	100.0	140	0	0	1	140	1	140	1.7e-73	284.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054733	MS.gene95856	0.858086	1.05E-62	-1.69E-46
MS.gene059893	MS.gene95856	0.812968	3.25E-51	-1.69E-46
MS.gene060856	MS.gene95856	0.825075	5.71E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene015616	MS.gene95856	PPI
MS.gene034319	MS.gene95856	PPI
MS.gene054733	MS.gene95856	PPI
MS.gene50088	MS.gene95856	PPI
MS.gene034318	MS.gene95856	PPI
MS.gene98113	MS.gene95856	PPI
MS.gene063979	MS.gene95856	PPI
MS.gene62652	MS.gene95856	PPI
MS.gene95856	MS.gene77759	PPI
MS.gene34285	MS.gene95856	PPI
MS.gene34284	MS.gene95856	PPI
MS.gene95856	MS.gene058560	PPI
MS.gene95856	MS.gene50088	PPI
MS.gene34283	MS.gene95856	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95856.t1	MTR_8g106080	100.000	140	0	1	140	1	140	1.17e-100	285
MS.gene95856.t1	MTR_8g106020	100.000	140	0	1	140	1	140	1.17e-100	285
MS.gene95856.t1	MTR_5g075240	99.286	140	1	1	140	1	140	2.63e-100	283
MS.gene95856.t1	MTR_4g085580	95.000	140	7	1	140	4	143	2.91e-96	273

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95856.t1	AT2G09990	89.928	139	14	2	140	8	146	5.16e-92	263
MS.gene95856.t1	AT5G18380	89.209	139	15	2	140	8	146	8.83e-92	262
MS.gene95856.t1	AT3G04230	84.173	139	22	2	140	8	146	1.84e-85	246
MS.gene95856.t1	AT5G18380	87.200	125	16	2	126	8	132	3.68e-79	230
MS.gene95856.t1	AT5G18380	87.037	108	14	2	109	8	115	1.14e-67	201

Find 38 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTAACGATAAGATTTC+TGG	0.210396	4.3:-1106949	None:intergenic
ACAGAGTAAGAAGGAGATTA+AGG	0.258952	4.3:+1106832	MS.gene95856:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	4.3:+1106902	MS.gene95856:CDS
CTGCAACAGCGTTCTTCTTC+CGG	0.375769	4.3:-1106568	None:intergenic
TCAGAGTATTGCGAAGGCTT+TGG	0.400647	4.3:+1106781	MS.gene95856:CDS
TACGAACCAATCCTCCTTCT+CGG	0.413918	4.3:+1106680	MS.gene95856:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	4.3:-1106911	None:intergenic
ATGGAACAGGTGCAATGCTT+TGG	0.453443	4.3:+1106545	MS.gene95856:CDS
GAAGCTCAGCAAGATGGAAC+AGG	0.488449	4.3:+1106532	None:intergenic
CAATGGCTGTCCAATCGAAC+TGG	0.489439	4.3:+1106628	MS.gene95856:CDS
CGCGGTCTCATCAAGATCAA+TGG	0.494184	4.3:+1106611	MS.gene95856:CDS
TCATATCAACTCCGGCGAAA+CGG	0.494924	4.3:-1106709	None:intergenic
CTCGGCAGACACCGTTTCGC+CGG	0.496313	4.3:+1106698	MS.gene95856:CDS
TCAGGCTCCACCAGTTCGAT+TGG	0.496656	4.3:-1106638	None:intergenic
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	4.3:+1106924	MS.gene95856:CDS
GCTTTGAAACGAAGAATCTC+AGG	0.503475	4.3:-1106656	None:intergenic
GTTTCGCCGGAGTTGATATG+AGG	0.511168	4.3:+1106711	MS.gene95856:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	4.3:+1106911	MS.gene95856:CDS
AGGATCCGTGTGAAGGGTGG+AGG	0.521481	4.3:+1106731	MS.gene95856:CDS
TGTTGATGAACAGAGTAAGA+AGG	0.534508	4.3:+1106823	MS.gene95856:CDS
CGAAACGGTGTCTGCCGAGA+AGG	0.541529	4.3:-1106694	None:intergenic
AACAGGTGCAATGCTTTGGC+CGG	0.546867	4.3:+1106549	MS.gene95856:CDS
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	4.3:+1106905	MS.gene95856:CDS
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	4.3:+1106593	MS.gene95856:CDS
AACGGTGTCTGCCGAGAAGG+AGG	0.579454	4.3:-1106691	None:intergenic
GCAGTTACCCACTGCAAGAG+AGG	0.580125	4.3:+1106587	MS.gene95856:CDS
TGGCTGTCCAATCGAACTGG+TGG	0.587642	4.3:+1106631	MS.gene95856:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	4.3:-1106896	None:intergenic
TGTCTGCCGAGAAGGAGGAT+TGG	0.604124	4.3:-1106686	None:intergenic
CTTCGCAATACTCTGACGAA+TGG	0.610127	4.3:-1106774	None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG	0.618077	4.3:-1106594	None:intergenic
CATTCGTCAGAGTATTGCGA+AGG	0.620290	4.3:+1106775	MS.gene95856:CDS
GACCGCGACCTCTCTTGCAG+TGG	0.624398	4.3:-1106595	None:intergenic
TGATATGAGGATCCGTGTGA+AGG	0.626071	4.3:+1106724	MS.gene95856:CDS
ATGAGGATCCGTGTGAAGGG+TGG	0.634725	4.3:+1106728	MS.gene95856:CDS
TGTGACCTCCACCCTTCACA+CGG	0.645312	4.3:-1106736	None:intergenic
ACGGATCCTCATATCAACTC+CGG	0.648153	4.3:-1106717	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.662094	4.3:+1106725	MS.gene95856:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAGGAGATTA+AGG	+	chr4.3:1106832-1106851	MS.gene95856:CDS	35.0%
!	TGTTGATGAACAGAGTAAGA+AGG	+	chr4.3:1106823-1106842	MS.gene95856:CDS	35.0%
!!	GCTTTGAAACGAAGAATCTC+AGG	-	chr4.3:1106659-1106678	None:intergenic	40.0%
	ACGGATCCTCATATCAACTC+CGG	-	chr4.3:1106720-1106739	None:intergenic	45.0%
	ATGGAACAGGTGCAATGCTT+TGG	+	chr4.3:1106545-1106564	MS.gene95856:CDS	45.0%
	CTTCGCAATACTCTGACGAA+TGG	-	chr4.3:1106777-1106796	None:intergenic	45.0%
	GATATGAGGATCCGTGTGAA+GGG	+	chr4.3:1106725-1106744	MS.gene95856:CDS	45.0%
	TACGAACCAATCCTCCTTCT+CGG	+	chr4.3:1106680-1106699	MS.gene95856:CDS	45.0%
	TCATATCAACTCCGGCGAAA+CGG	-	chr4.3:1106712-1106731	None:intergenic	45.0%
	TGATATGAGGATCCGTGTGA+AGG	+	chr4.3:1106724-1106743	MS.gene95856:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	+	chr4.3:1106905-1106924	MS.gene95856:CDS	45.0%
!!	CATTCGTCAGAGTATTGCGA+AGG	+	chr4.3:1106775-1106794	MS.gene95856:CDS	45.0%
!!	TCAGAGTATTGCGAAGGCTT+TGG	+	chr4.3:1106781-1106800	MS.gene95856:CDS	45.0%
	AACAGGTGCAATGCTTTGGC+CGG	+	chr4.3:1106549-1106568	MS.gene95856:CDS	50.0%
	CAATGGCTGTCCAATCGAAC+TGG	+	chr4.3:1106628-1106647	MS.gene95856:CDS	50.0%
	CCACGACCACCAAACTTCTT+AGG	-	chr4.3:1106914-1106933	None:intergenic	50.0%
	CGCGGTCTCATCAAGATCAA+TGG	+	chr4.3:1106611-1106630	MS.gene95856:CDS	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	+	chr4.3:1106902-1106921	MS.gene95856:CDS	50.0%
	CTGCAACAGCGTTCTTCTTC+CGG	-	chr4.3:1106571-1106590	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	-	chr4.3:1106899-1106918	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	+	chr4.3:1106911-1106930	MS.gene95856:CDS	50.0%
!!	GTTTCGCCGGAGTTGATATG+AGG	+	chr4.3:1106711-1106730	MS.gene95856:CDS	50.0%
	ATGAGGATCCGTGTGAAGGG+TGG	+	chr4.3:1106728-1106747	MS.gene95856:CDS	55.0%
	GCAGTTACCCACTGCAAGAG+AGG	+	chr4.3:1106587-1106606	MS.gene95856:CDS	55.0%
	TCAGGCTCCACCAGTTCGAT+TGG	-	chr4.3:1106641-1106660	None:intergenic	55.0%
	TGGCTGTCCAATCGAACTGG+TGG	+	chr4.3:1106631-1106650	MS.gene95856:CDS	55.0%
	TGTCTGCCGAGAAGGAGGAT+TGG	-	chr4.3:1106689-1106708	None:intergenic	55.0%
	TGTGACCTCCACCCTTCACA+CGG	-	chr4.3:1106739-1106758	None:intergenic	55.0%
	AACGGTGTCTGCCGAGAAGG+AGG	-	chr4.3:1106694-1106713	None:intergenic	60.0%
	ACCCACTGCAAGAGAGGTCG+CGG	+	chr4.3:1106593-1106612	MS.gene95856:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	-	chr4.3:1106597-1106616	None:intergenic	60.0%
	AGGATCCGTGTGAAGGGTGG+AGG	+	chr4.3:1106731-1106750	MS.gene95856:CDS	60.0%
	CGAAACGGTGTCTGCCGAGA+AGG	-	chr4.3:1106697-1106716	None:intergenic	60.0%
	CTCGGCAGACACCGTTTCGC+CGG	+	chr4.3:1106698-1106717	MS.gene95856:CDS	65.0%
	GACCGCGACCTCTCTTGCAG+TGG	-	chr4.3:1106598-1106617	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	+	chr4.3:1106924-1106943	MS.gene95856:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	1106545	1106967	1106545	ID=MS.gene95856
chr4.3	mRNA	1106545	1106967	1106545	ID=MS.gene95856.t1;Parent=MS.gene95856
chr4.3	exon	1106545	1106967	1106545	ID=MS.gene95856.t1.exon1;Parent=MS.gene95856.t1
chr4.3	CDS	1106545	1106967	1106545	ID=cds.MS.gene95856.t1;Parent=MS.gene95856.t1

Gene Sequence

>MS.gene95856

ATGGAACAGGTGCAATGCTTTGGCCGGAAGAAGAACGCTGTTGCAGTTACCCACTGCAAGAGAGGTCGCGGTCTCATCAAGATCAATGGCTGTCCAATCGAACTGGTGGAGCCTGAGATTCTTCGTTTCAAAGCATACGAACCAATCCTCCTTCTCGGCAGACACCGTTTCGCCGGAGTTGATATGAGGATCCGTGTGAAGGGTGGAGGTCACACTTCTCAGATCTATGCCATTCGTCAGAGTATTGCGAAGGCTTTGGTTGCTTATTATCAGAAATATGTTGATGAACAGAGTAAGAAGGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>MS.gene95856.t1

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKYVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR