AlfalfaGEDB | MS.gene34283

M. sativa cultivar XinJiangDaYe / MS.gene34283

KEGG: K02960
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34283.t1	XP_003631001.1	99.3	139	1	0	1	139	1	139	1.20E-72	282.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34283.t1	P46293	93.5	139	9	0	1	139	6	144	6.2e-70	264.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34283.t1	G7LBJ0	99.3	139	1	0	1	139	1	139	8.5e-73	282.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050252	MS.gene34283	0.865301	6.52E-65	-1.69E-46
MS.gene050290	MS.gene34283	0.882563	9.38E-71	-1.69E-46
MS.gene051850	MS.gene34283	0.804098	2.54E-49	-1.69E-46
MS.gene051904	MS.gene34283	0.828843	7.18E-55	-1.69E-46
MS.gene053952	MS.gene34283	0.80177	7.70E-49	-1.69E-46
MS.gene054937	MS.gene34283	0.866487	2.75E-65	-1.69E-46
MS.gene056399	MS.gene34283	0.803784	2.96E-49	-1.69E-46
MS.gene057052	MS.gene34283	0.846523	2.06E-59	-1.69E-46
MS.gene057206	MS.gene34283	0.802935	4.43E-49	-1.69E-46
MS.gene057235	MS.gene34283	0.810432	1.16E-50	-1.69E-46
MS.gene060856	MS.gene34283	0.836326	9.99E-57	-1.69E-46
MS.gene061145	MS.gene34283	0.867288	1.53E-65	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene34284	MS.gene34283	PPI
MS.gene34283	MS.gene50088	PPI
MS.gene34283	MS.gene054733	PPI
MS.gene34283	MS.gene034319	PPI
MS.gene034318	MS.gene34283	PPI
MS.gene34283	MS.gene62652	PPI
MS.gene063979	MS.gene34283	PPI
MS.gene34283	MS.gene77759	PPI
MS.gene34285	MS.gene34283	PPI
MS.gene34283	MS.gene98113	PPI
MS.gene34283	MS.gene015616	PPI
MS.gene34283	MS.gene058560	PPI
MS.gene34283	MS.gene95856	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34283.t1	MTR_8g106080	99.281	139	1	1	139	1	139	1.65e-99	281
MS.gene34283.t1	MTR_8g106020	99.281	139	1	1	139	1	139	1.65e-99	281
MS.gene34283.t1	MTR_5g075240	98.561	139	2	1	139	1	139	3.41e-99	281
MS.gene34283.t1	MTR_4g085580	94.245	139	8	1	139	4	142	4.90e-95	270

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34283.t1	AT2G09990	89.130	138	15	2	139	8	145	8.59e-91	259
MS.gene34283.t1	AT5G18380	88.406	138	16	2	139	8	145	1.27e-90	259
MS.gene34283.t1	AT3G04230	83.333	138	23	2	139	8	145	2.77e-84	243
MS.gene34283.t1	AT5G18380	86.400	125	17	2	126	8	132	1.41e-78	229
MS.gene34283.t1	AT5G18380	86.111	108	15	2	109	8	115	6.31e-67	199

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTACGATAAGATTTC+TGG	0.203036	4.1:-89791151	None:intergenic
ACAGAGTAAGAAAGAGATTA+AGG	0.239341	4.1:+89791034	MS.gene34283:CDS
CCGCAACGGCGTTCTTCTTC+CGG	0.308388	4.1:-89790770	None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG	0.344740	4.1:+89790857	MS.gene34283:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	4.1:+89791104	MS.gene34283:CDS
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	4.1:+89790983	MS.gene34283:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	4.1:-89791113	None:intergenic
TACGAACCAATCCTCCTTCT+CGG	0.444217	4.1:+89790882	MS.gene34283:CDS
GTTTCGCCGGCGTTGATATG+AGG	0.478501	4.1:+89790913	MS.gene34283:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	4.1:-89790858	None:intergenic
CTCGGAAGACACCGTTTCGC+CGG	0.484088	4.1:+89790900	MS.gene34283:CDS
ATGGAACAAGTGCAATGCTT+TGG	0.487256	4.1:+89790747	MS.gene34283:CDS
CAACGGCTGTCCAATCGAAC+TGG	0.488470	4.1:+89790830	MS.gene34283:CDS
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	4.1:+89791126	MS.gene34283:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	4.1:+89791113	MS.gene34283:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	4.1:+89790933	MS.gene34283:CDS
TCATATCAACGCCGGCGAAA+CGG	0.524265	4.1:-89790911	None:intergenic
TCAGGCTCAACCAGTTCGAT+TGG	0.524396	4.1:-89790840	None:intergenic
AACAAGTGCAATGCTTTGGC+CGG	0.546867	4.1:+89790751	MS.gene34283:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	4.1:-89790893	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	4.1:+89791107	MS.gene34283:CDS
GACCGCGACCTCTCTTGCAG+TGG	0.558828	4.1:-89790797	None:intergenic
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	4.1:+89790795	MS.gene34283:CDS
CATTCGTCAGAGTATTGCTA+AGG	0.576627	4.1:+89790977	MS.gene34283:CDS
TGATATGAGGATCCGTGTGA+AGG	0.579448	4.1:+89790926	MS.gene34283:CDS
GCGGTAACCCACTGCAAGAG+AGG	0.580471	4.1:+89790789	MS.gene34283:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	4.1:-89791098	None:intergenic
TGTCTTCCGAGAAGGAGGAT+TGG	0.592227	4.1:-89790888	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	4.1:+89790927	MS.gene34283:CDS
CTTGCAGTGGGTTACCGCAA+CGG	0.599991	4.1:-89790784	None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG	0.603590	4.1:-89790796	None:intergenic
CGCGGTCTGATCAAGATCAA+CGG	0.609302	4.1:+89790813	MS.gene34283:CDS
CTTAGCAATACTCTGACGAA+TGG	0.614188	4.1:-89790976	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	4.1:+89790930	MS.gene34283:CDS
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	4.1:-89790896	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.629480	4.1:-89790938	None:intergenic
CCGGAAGAAGAACGCCGTTG+CGG	0.651449	4.1:+89790770	MS.gene34283:CDS
ACGGATCCTCATATCAACGC+CGG	0.685813	4.1:-89790919	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAAGAGATTA+AGG	+	chr4.1:89791034-89791053	MS.gene34283:CDS	30.0%
	ATGGAACAAGTGCAATGCTT+TGG	+	chr4.1:89790747-89790766	MS.gene34283:CDS	40.0%
	CTTAGCAATACTCTGACGAA+TGG	-	chr4.1:89790979-89790998	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	+	chr4.1:89790977-89790996	MS.gene34283:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	+	chr4.1:89790983-89791002	MS.gene34283:CDS	40.0%
	AACAAGTGCAATGCTTTGGC+CGG	+	chr4.1:89790751-89790770	MS.gene34283:CDS	45.0%
	GATATGAGGATCCGTGTGAA+GGG	+	chr4.1:89790927-89790946	MS.gene34283:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	+	chr4.1:89790857-89790876	MS.gene34283:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	-	chr4.1:89790861-89790880	None:intergenic	45.0%
	TACGAACCAATCCTCCTTCT+CGG	+	chr4.1:89790882-89790901	MS.gene34283:CDS	45.0%
	TGATATGAGGATCCGTGTGA+AGG	+	chr4.1:89790926-89790945	MS.gene34283:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	+	chr4.1:89791107-89791126	MS.gene34283:CDS	45.0%
	ACGGATCCTCATATCAACGC+CGG	-	chr4.1:89790922-89790941	None:intergenic	50.0%
	CCACGACCACCAAACTTCTT+AGG	-	chr4.1:89791116-89791135	None:intergenic	50.0%
	CGCGGTCTGATCAAGATCAA+CGG	+	chr4.1:89790813-89790832	MS.gene34283:CDS	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	+	chr4.1:89791104-89791123	MS.gene34283:CDS	50.0%
	TCAGGCTCAACCAGTTCGAT+TGG	-	chr4.1:89790843-89790862	None:intergenic	50.0%
	TCATATCAACGCCGGCGAAA+CGG	-	chr4.1:89790914-89790933	None:intergenic	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	-	chr4.1:89790891-89790910	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	-	chr4.1:89791101-89791120	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	+	chr4.1:89791113-89791132	MS.gene34283:CDS	50.0%
	AACGGTGTCTTCCGAGAAGG+AGG	-	chr4.1:89790896-89790915	None:intergenic	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	+	chr4.1:89790930-89790949	MS.gene34283:CDS	55.0%
	CAACGGCTGTCCAATCGAAC+TGG	+	chr4.1:89790830-89790849	MS.gene34283:CDS	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	-	chr4.1:89790899-89790918	None:intergenic	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	-	chr4.1:89790941-89790960	None:intergenic	55.0%
!!	CTTGCAGTGGGTTACCGCAA+CGG	-	chr4.1:89790787-89790806	None:intergenic	55.0%
!!	GTTTCGCCGGCGTTGATATG+AGG	+	chr4.1:89790913-89790932	MS.gene34283:CDS	55.0%
	ACCCACTGCAAGAGAGGTCG+CGG	+	chr4.1:89790795-89790814	MS.gene34283:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	-	chr4.1:89790799-89790818	None:intergenic	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	+	chr4.1:89790933-89790952	MS.gene34283:CDS	60.0%
	CCGCAACGGCGTTCTTCTTC+CGG	-	chr4.1:89790773-89790792	None:intergenic	60.0%
	CCGGAAGAAGAACGCCGTTG+CGG	+	chr4.1:89790770-89790789	MS.gene34283:CDS	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	+	chr4.1:89790900-89790919	MS.gene34283:CDS	60.0%
	GCGGTAACCCACTGCAAGAG+AGG	+	chr4.1:89790789-89790808	MS.gene34283:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	-	chr4.1:89790800-89790819	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	+	chr4.1:89791126-89791145	MS.gene34283:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	89790747	89791164	89790747	ID=MS.gene34283
chr4.1	mRNA	89790747	89791164	89790747	ID=MS.gene34283.t1;Parent=MS.gene34283
chr4.1	exon	89790747	89791164	89790747	ID=MS.gene34283.t1.exon1;Parent=MS.gene34283.t1
chr4.1	CDS	89790747	89791164	89790747	ID=cds.MS.gene34283.t1;Parent=MS.gene34283.t1

Gene Sequence

>MS.gene34283

ATGGAACAAGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCGGTAACCCACTGCAAGAGAGGTCGCGGTCTGATCAAGATCAACGGCTGTCCAATCGAACTGGTTGAGCCTGAGATTCTTCGTTTCAAGGCATACGAACCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGCGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCTCAGATCTACGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTACTATCAGAAATTTGTTGATGAACAGAGTAAGAAAGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATC

Protein sequence

>MS.gene34283.t1

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKFVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSY