AlfalfaGEDB | MS.gene34285

M. sativa cultivar XinJiangDaYe / MS.gene34285

KEGG: K02960
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34285.t1	XP_003631001.1	99.3	140	1	0	1	140	1	140	5.40E-73	283.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34285.t1	P46293	93.6	140	9	0	1	140	6	145	2.8e-70	265.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34285.t1	G7LBJ0	99.3	140	1	0	1	140	1	140	3.9e-73	283.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050252	MS.gene34285	0.869195	3.71E-66	-1.69E-46
MS.gene050290	MS.gene34285	0.870255	1.68E-66	-1.69E-46
MS.gene051904	MS.gene34285	0.841651	4.18E-58	-1.69E-46
MS.gene054937	MS.gene34285	0.854447	1.22E-61	-1.69E-46
MS.gene057052	MS.gene34285	0.836897	7.15E-57	-1.69E-46
MS.gene057206	MS.gene34285	0.804746	1.86E-49	-1.69E-46
MS.gene057235	MS.gene34285	0.832525	9.00E-56	-1.69E-46
MS.gene060856	MS.gene34285	0.834124	3.59E-56	-1.69E-46
MS.gene061145	MS.gene34285	0.840392	8.94E-58	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene34284	MS.gene34285	PPI
MS.gene34285	MS.gene054733	PPI
MS.gene34285	MS.gene034319	PPI
MS.gene34285	MS.gene62652	PPI
MS.gene34285	MS.gene77759	PPI
MS.gene034318	MS.gene34285	PPI
MS.gene34285	MS.gene98113	PPI
MS.gene34285	MS.gene34283	PPI
MS.gene063979	MS.gene34285	PPI
MS.gene34285	MS.gene015616	PPI
MS.gene34285	MS.gene058560	PPI
MS.gene34285	MS.gene95856	PPI
MS.gene34285	MS.gene50088	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34285.t1	MTR_8g106080	99.286	140	1	1	140	1	140	3.04e-100	283
MS.gene34285.t1	MTR_8g106020	99.286	140	1	1	140	1	140	3.04e-100	283
MS.gene34285.t1	MTR_5g075240	98.571	140	2	1	140	1	140	7.72e-100	282
MS.gene34285.t1	MTR_4g085580	94.286	140	8	1	140	4	143	9.84e-96	272

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34285.t1	AT2G09990	89.209	139	15	2	140	8	146	1.84e-91	261
MS.gene34285.t1	AT5G18380	88.489	139	16	2	140	8	146	3.12e-91	261
MS.gene34285.t1	AT3G04230	83.453	139	23	2	140	8	146	5.94e-85	245
MS.gene34285.t1	AT5G18380	86.400	125	17	2	126	8	132	1.60e-78	229
MS.gene34285.t1	AT5G18380	86.111	108	15	2	109	8	115	5.33e-67	199

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTAACGATAAGATTTC+TGG	0.210396	4.1:+89827261	None:intergenic
ACAGAGTAAGAAAGAGATTA+AGG	0.239341	4.1:-89827378	MS.gene34285:CDS
CCGCAACGGCGTTCTTCTTC+CGG	0.308388	4.1:+89827642	None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG	0.344740	4.1:-89827555	MS.gene34285:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	4.1:-89827308	MS.gene34285:CDS
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	4.1:-89827429	MS.gene34285:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	4.1:+89827299	None:intergenic
TACGAACCAATCCTCCTTCT+CGG	0.444217	4.1:-89827530	MS.gene34285:CDS
GTTTCGCCGGCGTTGATATG+AGG	0.478501	4.1:-89827499	MS.gene34285:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.478888	4.1:+89827554	None:intergenic
CTCGGAAGACACCGTTTCGC+CGG	0.484088	4.1:-89827512	MS.gene34285:CDS
ATGGAACAAGTGCAATGCTT+TGG	0.487256	4.1:-89827665	MS.gene34285:CDS
CAACGGCTGTCCAATCGAAC+TGG	0.488470	4.1:-89827582	MS.gene34285:CDS
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	4.1:-89827286	MS.gene34285:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	4.1:-89827299	MS.gene34285:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	4.1:-89827479	MS.gene34285:CDS
TCATATCAACGCCGGCGAAA+CGG	0.524265	4.1:+89827501	None:intergenic
TCAGGCTCAACCAGTTCGAT+TGG	0.524396	4.1:+89827572	None:intergenic
AACAAGTGCAATGCTTTGGC+CGG	0.546867	4.1:-89827661	MS.gene34285:CDS
AACGGTGTCTTCCGAGAAGG+AGG	0.549270	4.1:+89827519	None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	4.1:-89827305	MS.gene34285:CDS
GACCGCGACCTCTCTTGCAG+TGG	0.558828	4.1:+89827615	None:intergenic
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	4.1:-89827617	MS.gene34285:CDS
CATTCGTCAGAGTATTGCTA+AGG	0.576627	4.1:-89827435	MS.gene34285:CDS
TGATATGAGGATCCGTGTGA+AGG	0.579448	4.1:-89827486	MS.gene34285:CDS
GCGGTAACCCACTGCAAGAG+AGG	0.580471	4.1:-89827623	MS.gene34285:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	4.1:+89827314	None:intergenic
TGTCTTCCGAGAAGGAGGAT+TGG	0.592227	4.1:+89827524	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	4.1:-89827485	MS.gene34285:CDS
CTTGCAGTGGGTTACCGCAA+CGG	0.599991	4.1:+89827628	None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG	0.603590	4.1:+89827616	None:intergenic
CGCGGTCTGATCAAGATCAA+CGG	0.609302	4.1:-89827599	MS.gene34285:CDS
CTTAGCAATACTCTGACGAA+TGG	0.614188	4.1:+89827436	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	4.1:-89827482	MS.gene34285:CDS
CGAAACGGTGTCTTCCGAGA+AGG	0.622095	4.1:+89827516	None:intergenic
TGTGACCTCCTCCCTTCACA+CGG	0.629480	4.1:+89827474	None:intergenic
CCGGAAGAAGAACGCCGTTG+CGG	0.651449	4.1:-89827642	MS.gene34285:CDS
ACGGATCCTCATATCAACGC+CGG	0.685813	4.1:+89827493	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAAGAGATTA+AGG	-	chr4.1:89827552-89827571	MS.gene34285:CDS	30.0%
	ATGGAACAAGTGCAATGCTT+TGG	-	chr4.1:89827265-89827284	MS.gene34285:CDS	40.0%
	CTTAGCAATACTCTGACGAA+TGG	+	chr4.1:89827497-89827516	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	-	chr4.1:89827495-89827514	MS.gene34285:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	-	chr4.1:89827501-89827520	MS.gene34285:CDS	40.0%
	AACAAGTGCAATGCTTTGGC+CGG	-	chr4.1:89827269-89827288	MS.gene34285:CDS	45.0%
	GATATGAGGATCCGTGTGAA+GGG	-	chr4.1:89827445-89827464	MS.gene34285:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	-	chr4.1:89827375-89827394	MS.gene34285:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	+	chr4.1:89827379-89827398	None:intergenic	45.0%
	TACGAACCAATCCTCCTTCT+CGG	-	chr4.1:89827400-89827419	MS.gene34285:CDS	45.0%
	TGATATGAGGATCCGTGTGA+AGG	-	chr4.1:89827444-89827463	MS.gene34285:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	-	chr4.1:89827625-89827644	MS.gene34285:CDS	45.0%
	ACGGATCCTCATATCAACGC+CGG	+	chr4.1:89827440-89827459	None:intergenic	50.0%
	CCACGACCACCAAACTTCTT+AGG	+	chr4.1:89827634-89827653	None:intergenic	50.0%
	CGCGGTCTGATCAAGATCAA+CGG	-	chr4.1:89827331-89827350	MS.gene34285:CDS	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	-	chr4.1:89827622-89827641	MS.gene34285:CDS	50.0%
	TCAGGCTCAACCAGTTCGAT+TGG	+	chr4.1:89827361-89827380	None:intergenic	50.0%
	TCATATCAACGCCGGCGAAA+CGG	+	chr4.1:89827432-89827451	None:intergenic	50.0%
	TGTCTTCCGAGAAGGAGGAT+TGG	+	chr4.1:89827409-89827428	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	+	chr4.1:89827619-89827638	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	-	chr4.1:89827631-89827650	MS.gene34285:CDS	50.0%
	AACGGTGTCTTCCGAGAAGG+AGG	+	chr4.1:89827414-89827433	None:intergenic	55.0%
	ATGAGGATCCGTGTGAAGGG+AGG	-	chr4.1:89827448-89827467	MS.gene34285:CDS	55.0%
	CAACGGCTGTCCAATCGAAC+TGG	-	chr4.1:89827348-89827367	MS.gene34285:CDS	55.0%
	CGAAACGGTGTCTTCCGAGA+AGG	+	chr4.1:89827417-89827436	None:intergenic	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	+	chr4.1:89827459-89827478	None:intergenic	55.0%
!!	CTTGCAGTGGGTTACCGCAA+CGG	+	chr4.1:89827305-89827324	None:intergenic	55.0%
!!	GTTTCGCCGGCGTTGATATG+AGG	-	chr4.1:89827431-89827450	MS.gene34285:CDS	55.0%
	ACCCACTGCAAGAGAGGTCG+CGG	-	chr4.1:89827313-89827332	MS.gene34285:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	+	chr4.1:89827317-89827336	None:intergenic	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	-	chr4.1:89827451-89827470	MS.gene34285:CDS	60.0%
	CCGCAACGGCGTTCTTCTTC+CGG	+	chr4.1:89827291-89827310	None:intergenic	60.0%
	CCGGAAGAAGAACGCCGTTG+CGG	-	chr4.1:89827288-89827307	MS.gene34285:CDS	60.0%
	CTCGGAAGACACCGTTTCGC+CGG	-	chr4.1:89827418-89827437	MS.gene34285:CDS	60.0%
	GCGGTAACCCACTGCAAGAG+AGG	-	chr4.1:89827307-89827326	MS.gene34285:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	+	chr4.1:89827318-89827337	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	-	chr4.1:89827644-89827663	MS.gene34285:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	89827265	89827687	89827265	ID=MS.gene34285
chr4.1	mRNA	89827265	89827687	89827265	ID=MS.gene34285.t1;Parent=MS.gene34285
chr4.1	exon	89827265	89827687	89827265	ID=MS.gene34285.t1.exon1;Parent=MS.gene34285.t1
chr4.1	CDS	89827265	89827687	89827265	ID=cds.MS.gene34285.t1;Parent=MS.gene34285.t1

Gene Sequence

>MS.gene34285

ATGGAACAAGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCGGTAACCCACTGCAAGAGAGGTCGCGGTCTGATCAAGATCAACGGCTGTCCAATCGAACTGGTTGAGCCTGAGATTCTTCGTTTCAAGGCATACGAACCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGCGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTCTCAGATCTACGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTACTATCAGAAATTTGTTGATGAACAGAGTAAGAAAGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>MS.gene34285.t1

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKFVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR