AlfalfaGEDB | MS.gene77759

M. sativa cultivar XinJiangDaYe / MS.gene77759

KEGG: K02960
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00380.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77759.t1	XP_003631001.1	97.1	140	4	0	1	140	1	140	1.50E-70	275.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77759.t1	P46293	91.4	140	12	0	1	140	6	145	7.6e-68	257.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77759.t1	G7LBJ0	97.1	140	4	0	1	140	1	140	1.1e-70	275.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene77759	0.806294	8.82E-50	-1.69E-46
MS.gene049178	MS.gene77759	0.831219	1.89E-55	-1.69E-46
MS.gene050114	MS.gene77759	0.812844	3.46E-51	-1.69E-46
MS.gene050317	MS.gene77759	0.802736	4.87E-49	-1.69E-46
MS.gene050439	MS.gene77759	0.82103	5.01E-53	-1.69E-46
MS.gene050838	MS.gene77759	0.847898	8.64E-60	-1.69E-46
MS.gene051003	MS.gene77759	0.81949	1.13E-52	-1.69E-46
MS.gene051343	MS.gene77759	0.815342	9.71E-52	-1.69E-46
MS.gene051700	MS.gene77759	0.824969	6.05E-54	-1.69E-46
MS.gene051748	MS.gene77759	0.800978	1.12E-48	-1.69E-46
MS.gene051757	MS.gene77759	0.823696	1.21E-53	-1.69E-46
MS.gene051790	MS.gene77759	0.801119	1.05E-48	-1.69E-46
MS.gene05188	MS.gene77759	0.839055	1.99E-57	-1.69E-46
MS.gene051903	MS.gene77759	0.809751	1.62E-50	-1.69E-46
MS.gene051975	MS.gene77759	0.844272	8.38E-59	-1.69E-46
MS.gene052434	MS.gene77759	0.803984	2.69E-49	-1.69E-46
MS.gene052947	MS.gene77759	0.831004	2.13E-55	-1.69E-46
MS.gene053058	MS.gene77759	0.806427	8.27E-50	-1.69E-46
MS.gene053381	MS.gene77759	0.826811	2.21E-54	-1.69E-46
MS.gene053564	MS.gene77759	0.82318	1.59E-53	-1.69E-46
MS.gene053786	MS.gene77759	0.820289	7.42E-53	-1.69E-46
MS.gene054576	MS.gene77759	0.802545	5.33E-49	-1.69E-46
MS.gene055311	MS.gene77759	0.815405	9.40E-52	-1.69E-46
MS.gene055313	MS.gene77759	0.821687	3.54E-53	-1.69E-46
MS.gene055314	MS.gene77759	0.800787	1.22E-48	-1.69E-46
MS.gene055513	MS.gene77759	0.830083	3.58E-55	-1.69E-46
MS.gene055603	MS.gene77759	0.848715	5.14E-60	-1.69E-46
MS.gene056390	MS.gene77759	0.804035	2.62E-49	-1.69E-46
MS.gene056403	MS.gene77759	0.830656	2.60E-55	-1.69E-46
MS.gene056605	MS.gene77759	0.810778	9.73E-51	-1.69E-46
MS.gene056631	MS.gene77759	0.803627	3.19E-49	-1.69E-46
MS.gene056638	MS.gene77759	0.804922	1.71E-49	-1.69E-46
MS.gene056787	MS.gene77759	0.801449	8.96E-49	-1.69E-46
MS.gene056986	MS.gene77759	0.841825	3.76E-58	-1.69E-46
MS.gene057094	MS.gene77759	0.816597	5.09E-52	-1.69E-46
MS.gene05781	MS.gene77759	0.801875	7.33E-49	-1.69E-46
MS.gene057890	MS.gene77759	-0.811177	7.98E-51	-1.69E-46
MS.gene058084	MS.gene77759	0.800928	1.14E-48	-1.69E-46
MS.gene058654	MS.gene77759	0.803267	3.78E-49	-1.69E-46
MS.gene058758	MS.gene77759	0.800572	1.35E-48	-1.69E-46
MS.gene058852	MS.gene77759	0.808022	3.80E-50	-1.69E-46
MS.gene058906	MS.gene77759	0.813714	2.23E-51	-1.69E-46
MS.gene059170	MS.gene77759	0.823474	1.36E-53	-1.69E-46
MS.gene059171	MS.gene77759	0.816475	5.43E-52	-1.69E-46
MS.gene059227	MS.gene77759	0.817367	3.42E-52	-1.69E-46
MS.gene059360	MS.gene77759	0.800382	1.48E-48	-1.69E-46
MS.gene06001	MS.gene77759	0.849773	2.61E-60	-1.69E-46
MS.gene06010	MS.gene77759	0.810154	1.33E-50	-1.69E-46
MS.gene06011	MS.gene77759	0.856102	4.04E-62	-1.69E-46
MS.gene060429	MS.gene77759	0.816986	4.17E-52	-1.69E-46
MS.gene060763	MS.gene77759	0.806559	7.76E-50	-1.69E-46
MS.gene060869	MS.gene77759	0.838758	2.38E-57	-1.69E-46
MS.gene060870	MS.gene77759	0.828418	9.09E-55	-1.69E-46
MS.gene061100	MS.gene77759	0.805203	1.49E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene034319	MS.gene77759	PPI
MS.gene054733	MS.gene77759	PPI
MS.gene034318	MS.gene77759	PPI
MS.gene98113	MS.gene77759	PPI
MS.gene62652	MS.gene77759	PPI
MS.gene063979	MS.gene77759	PPI
MS.gene34285	MS.gene77759	PPI
MS.gene95856	MS.gene77759	PPI
MS.gene50088	MS.gene77759	PPI
MS.gene058560	MS.gene77759	PPI
MS.gene34283	MS.gene77759	PPI
MS.gene015616	MS.gene77759	PPI
MS.gene34284	MS.gene77759	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77759.t1	MTR_8g106080	97.143	140	4	1	140	1	140	6.24e-97	275
MS.gene77759.t1	MTR_8g106020	97.143	140	4	1	140	1	140	6.24e-97	275
MS.gene77759.t1	MTR_5g075240	96.429	140	5	1	140	1	140	1.81e-96	274
MS.gene77759.t1	MTR_4g085580	92.857	140	10	1	140	4	143	1.15e-92	264

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77759.t1	AT2G09990	86.429	140	19	1	140	7	146	3.20e-88	253
MS.gene77759.t1	AT5G18380	85.714	140	20	1	140	7	146	4.60e-88	253
MS.gene77759.t1	AT3G04230	81.295	139	26	2	140	8	146	1.11e-81	237
MS.gene77759.t1	AT5G18380	84.800	125	19	2	126	8	132	5.65e-76	222
MS.gene77759.t1	AT5G18380	84.259	108	17	2	109	8	115	2.14e-64	193

Find 37 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTAACGATAATATTTC+TGG	0.207731	8.1:+59754672	None:intergenic
ACAGAGTAAGAAAGAGATTA+AGG	0.239341	8.1:-59754789	MS.gene77759:CDS
CCGCAACGGCGTTCTTCTTC+CGG	0.308388	8.1:+59755053	None:intergenic
GCCTGAGATTCTTCGTTTCA+AGG	0.344740	8.1:-59754966	MS.gene77759:CDS
CGCTGTGAGCCTAAGAAGTT+TGG	0.359075	8.1:-59754719	MS.gene77759:CDS
TCAGAGTATTGCTAAGGCTT+TGG	0.386737	8.1:-59754840	MS.gene77759:CDS
CCACGACCACCAAACTTCTT+AGG	0.436009	8.1:+59754710	None:intergenic
GTTTCGCCGGCGTTGATATG+AGG	0.478501	8.1:-59754910	MS.gene77759:CDS
GCCTTGAAACGAAGAATCTC+AGG	0.479199	8.1:+59754965	None:intergenic
CGAAACGGTGTCTTTCTAGA+AGG	0.480472	8.1:+59754927	None:intergenic
ATGGAACAAGTGCAATGCTT+TGG	0.487256	8.1:-59755076	MS.gene77759:CDS
CAACGGCTGTCCAATCGAAC+TGG	0.488470	8.1:-59754993	MS.gene77759:CDS
GTGGTCGTGGTGCTCGTGCT+AGG	0.502073	8.1:-59754697	MS.gene77759:CDS
CCTAAGAAGTTTGGTGGTCG+TGG	0.511400	8.1:-59754710	MS.gene77759:CDS
AGGATCCGTGTGAAGGGAGG+AGG	0.521481	8.1:-59754890	MS.gene77759:CDS
TCATATCAACGCCGGCGAAA+CGG	0.524265	8.1:+59754912	None:intergenic
TCAGGCTCAACCAGTTCGAT+TGG	0.524396	8.1:+59754983	None:intergenic
TGTCTTTCTAGAAGGAGGAT+TGG	0.532576	8.1:+59754935	None:intergenic
CTAGAAAGACACCGTTTCGC+CGG	0.532820	8.1:-59754923	MS.gene77759:CDS
AACAAGTGCAATGCTTTGGC+CGG	0.546867	8.1:-59755072	MS.gene77759:CDS
TGTGAGCCTAAGAAGTTTGG+TGG	0.558448	8.1:-59754716	MS.gene77759:CDS
GACCGCGACCTCTCTTGCAG+TGG	0.558828	8.1:+59755026	None:intergenic
ACCCACTGCAAGAGAGGTCG+CGG	0.562945	8.1:-59755028	MS.gene77759:CDS
AACGGTGTCTTTCTAGAAGG+AGG	0.569043	8.1:+59754930	None:intergenic
CATTCGTCAGAGTATTGCTA+AGG	0.576627	8.1:-59754846	MS.gene77759:CDS
TGATATGAGGATCCGTGTGA+AGG	0.579448	8.1:-59754897	MS.gene77759:CDS
GCGGTAACCCACTGCAAGAG+AGG	0.580471	8.1:-59755034	MS.gene77759:CDS
TTCTTAGGCTCACAGCGTCT+AGG	0.590494	8.1:+59754725	None:intergenic
GATATGAGGATCCGTGTGAA+GGG	0.594162	8.1:-59754896	MS.gene77759:CDS
CTTGCAGTGGGTTACCGCAA+CGG	0.599991	8.1:+59755039	None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG	0.603590	8.1:+59755027	None:intergenic
CGCGGTCTGATCAAGATCAA+CGG	0.609302	8.1:-59755010	MS.gene77759:CDS
CTTAGCAATACTCTGACGAA+TGG	0.614188	8.1:+59754847	None:intergenic
ATGAGGATCCGTGTGAAGGG+AGG	0.615322	8.1:-59754893	MS.gene77759:CDS
TGTGACCTCCTCCCTTCACA+CGG	0.631500	8.1:+59754885	None:intergenic
CCGGAAGAAGAACGCCGTTG+CGG	0.651449	8.1:-59755053	MS.gene77759:CDS
ACGGATCCTCATATCAACGC+CGG	0.685813	8.1:+59754904	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAGAGTAAGAAAGAGATTA+AGG	-	chr8.1:59754963-59754982	MS.gene77759:CDS	30.0%
	ATGGAACAAGTGCAATGCTT+TGG	-	chr8.1:59754676-59754695	MS.gene77759:CDS	40.0%
	CTTAGCAATACTCTGACGAA+TGG	+	chr8.1:59754908-59754927	None:intergenic	40.0%
!	TGTCTTTCTAGAAGGAGGAT+TGG	+	chr8.1:59754820-59754839	None:intergenic	40.0%
!!	CATTCGTCAGAGTATTGCTA+AGG	-	chr8.1:59754906-59754925	MS.gene77759:CDS	40.0%
!!	TCAGAGTATTGCTAAGGCTT+TGG	-	chr8.1:59754912-59754931	MS.gene77759:CDS	40.0%
	AACAAGTGCAATGCTTTGGC+CGG	-	chr8.1:59754680-59754699	MS.gene77759:CDS	45.0%
	CGAAACGGTGTCTTTCTAGA+AGG	+	chr8.1:59754828-59754847	None:intergenic	45.0%
	GATATGAGGATCCGTGTGAA+GGG	-	chr8.1:59754856-59754875	MS.gene77759:CDS	45.0%
	GCCTGAGATTCTTCGTTTCA+AGG	-	chr8.1:59754786-59754805	MS.gene77759:CDS	45.0%
	GCCTTGAAACGAAGAATCTC+AGG	+	chr8.1:59754790-59754809	None:intergenic	45.0%
	TGATATGAGGATCCGTGTGA+AGG	-	chr8.1:59754855-59754874	MS.gene77759:CDS	45.0%
	TGTGAGCCTAAGAAGTTTGG+TGG	-	chr8.1:59755036-59755055	MS.gene77759:CDS	45.0%
!	AACGGTGTCTTTCTAGAAGG+AGG	+	chr8.1:59754825-59754844	None:intergenic	45.0%
	ACGGATCCTCATATCAACGC+CGG	+	chr8.1:59754851-59754870	None:intergenic	50.0%
	CCACGACCACCAAACTTCTT+AGG	+	chr8.1:59755045-59755064	None:intergenic	50.0%
	CGCGGTCTGATCAAGATCAA+CGG	-	chr8.1:59754742-59754761	MS.gene77759:CDS	50.0%
	CGCTGTGAGCCTAAGAAGTT+TGG	-	chr8.1:59755033-59755052	MS.gene77759:CDS	50.0%
	CTAGAAAGACACCGTTTCGC+CGG	-	chr8.1:59754829-59754848	MS.gene77759:CDS	50.0%
	TCAGGCTCAACCAGTTCGAT+TGG	+	chr8.1:59754772-59754791	None:intergenic	50.0%
	TCATATCAACGCCGGCGAAA+CGG	+	chr8.1:59754843-59754862	None:intergenic	50.0%
	TTCTTAGGCTCACAGCGTCT+AGG	+	chr8.1:59755030-59755049	None:intergenic	50.0%
!	CCTAAGAAGTTTGGTGGTCG+TGG	-	chr8.1:59755042-59755061	MS.gene77759:CDS	50.0%
	ATGAGGATCCGTGTGAAGGG+AGG	-	chr8.1:59754859-59754878	MS.gene77759:CDS	55.0%
	CAACGGCTGTCCAATCGAAC+TGG	-	chr8.1:59754759-59754778	MS.gene77759:CDS	55.0%
	TGTGACCTCCTCCCTTCACA+CGG	+	chr8.1:59754870-59754889	None:intergenic	55.0%
!!	CTTGCAGTGGGTTACCGCAA+CGG	+	chr8.1:59754716-59754735	None:intergenic	55.0%
!!	GTTTCGCCGGCGTTGATATG+AGG	-	chr8.1:59754842-59754861	MS.gene77759:CDS	55.0%
	ACCCACTGCAAGAGAGGTCG+CGG	-	chr8.1:59754724-59754743	MS.gene77759:CDS	60.0%
	ACCGCGACCTCTCTTGCAGT+GGG	+	chr8.1:59754728-59754747	None:intergenic	60.0%
	AGGATCCGTGTGAAGGGAGG+AGG	-	chr8.1:59754862-59754881	MS.gene77759:CDS	60.0%
	CCGCAACGGCGTTCTTCTTC+CGG	+	chr8.1:59754702-59754721	None:intergenic	60.0%
	CCGGAAGAAGAACGCCGTTG+CGG	-	chr8.1:59754699-59754718	MS.gene77759:CDS	60.0%
	GCGGTAACCCACTGCAAGAG+AGG	-	chr8.1:59754718-59754737	MS.gene77759:CDS	60.0%
	GACCGCGACCTCTCTTGCAG+TGG	+	chr8.1:59754729-59754748	None:intergenic	65.0%
	GTGGTCGTGGTGCTCGTGCT+AGG	-	chr8.1:59755055-59755074	MS.gene77759:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	59754676	59755098	59754676	ID=MS.gene77759
chr8.1	mRNA	59754676	59755098	59754676	ID=MS.gene77759.t1;Parent=MS.gene77759
chr8.1	exon	59754676	59755098	59754676	ID=MS.gene77759.t1.exon1;Parent=MS.gene77759.t1
chr8.1	CDS	59754676	59755098	59754676	ID=cds.MS.gene77759.t1;Parent=MS.gene77759.t1

Gene Sequence

>MS.gene77759

ATGGAACAAGTGCAATGCTTTGGCCGGAAGAAGAACGCCGTTGCGGTAACCCACTGCAAGAGAGGTCGCGGTCTGATCAAGATCAACGGCTGTCCAATCGAACTGGTTGAGCCTGAGATTCTTCGTTTCAAGGCATATGAACCAATCCTCCTTCTAGAAAGACACCGTTTCGCCGGCGTTGATATGAGGATCCGTGTGAAGGGAGGAGGTCACACTTTTCAGATCTACGCCATTCGTCAGAGTATTGCTAAGGCTTTGGTTGCTTACTATCAGAAATTTGTTGATGAACAGAGTAAGAAAGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATATTATCGTTAG

Protein sequence

>MS.gene77759.t1

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLERHRFAGVDMRIRVKGGGHTFQIYAIRQSIAKALVAYYQKFVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKYYR