AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015821

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015821.t1 MTR_8g102470 97.626 337 3 2 1 337 1 332 0.0 661
MS.gene015821.t1 MTR_8g102470 99.351 308 2 0 30 337 1 308 0.0 619
MS.gene015821.t1 MTR_8g088900 49.000 300 147 3 34 328 7 305 3.74e-95 287
MS.gene015821.t1 MTR_2g099910 30.195 308 207 3 25 325 24 330 1.74e-40 145
MS.gene015821.t1 MTR_8g028120 31.557 244 164 3 42 284 2 243 1.26e-36 133
MS.gene015821.t1 MTR_2g099910 31.004 229 150 3 25 246 24 251 3.59e-29 113
MS.gene015821.t1 MTR_6g069685 27.742 310 217 4 33 336 315 623 1.20e-24 105
MS.gene015821.t1 MTR_6g069685 23.986 296 216 6 43 332 7 299 4.82e-14 73.6
MS.gene015821.t1 MTR_2g030400 25.904 332 212 8 3 307 1 325 8.74e-24 100
MS.gene015821.t1 MTR_2g099910 31.959 194 124 3 25 211 24 216 2.62e-23 97.1
MS.gene015821.t1 MTR_2g030400 25.610 328 210 8 3 303 1 321 1.77e-22 96.3
MS.gene015821.t1 MTR_2g030400 26.812 276 175 6 3 258 1 269 1.87e-19 87.4
MS.gene015821.t1 MTR_2g030400 27.953 254 156 6 3 236 1 247 2.28e-19 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015821.t1 AT4G29120 72.141 341 80 2 1 335 1 332 6.85e-180 501
MS.gene015821.t1 AT1G71180 48.684 304 147 3 30 331 22 318 5.73e-84 257
MS.gene015821.t1 AT1G71170 46.711 304 155 3 30 331 1 299 1.09e-80 248
MS.gene015821.t1 AT1G17650 29.664 327 221 4 6 325 27 351 4.50e-41 147
MS.gene015821.t1 AT1G17650 30.986 284 194 2 43 325 10 292 1.74e-40 144
MS.gene015821.t1 AT3G25530 30.249 281 194 2 42 321 2 281 1.80e-37 136
MS.gene015821.t1 AT3G25530 28.826 281 187 2 42 321 2 270 4.16e-34 127
MS.gene015821.t1 AT3G25530 33.498 203 133 2 42 243 2 203 5.82e-31 116
MS.gene015821.t1 AT4G20930 29.210 291 181 7 43 311 40 327 7.71e-28 111
MS.gene015821.t1 AT1G18270 26.603 312 220 4 25 332 310 616 1.72e-23 102
MS.gene015821.t1 AT1G18270 21.034 290 221 5 43 327 5 291 6.07e-12 67.4
MS.gene015821.t1 AT1G18270 26.603 312 220 4 25 332 310 616 2.17e-23 102
MS.gene015821.t1 AT1G18270 21.034 290 221 5 43 327 5 291 6.97e-12 67.0
MS.gene015821.t1 AT1G18270 26.603 312 220 4 25 332 310 616 2.26e-23 101
MS.gene015821.t1 AT1G18270 21.034 290 221 5 43 327 5 291 8.25e-12 67.0
MS.gene015821.t1 AT1G18270 26.603 312 220 4 25 332 311 617 2.26e-23 101
MS.gene015821.t1 AT4G20930 28.063 253 157 7 81 311 6 255 2.99e-20 89.4

Find 134 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTCATGGTGAAGGTAATT+TGG 0.154817 4.2:+2620419 MS.gene015821:CDS
GGGTACTCAGGCTCTTATTT+TGG 0.213438 4.2:+2620440 MS.gene015821:CDS
GCGGGTGTTTGATGGGGTTA+TGG 0.236145 4.2:-2619599 None:intergenic
TTTGAAAGAATGTGAGAAAA+TGG 0.246727 4.2:+2620344 MS.gene015821:CDS
CCGGCGAGTGCGCCGGTGTT+TGG 0.261173 4.2:-2619844 None:intergenic
TCAATCGACGCTCCCGTTTC+CGG 0.279408 4.2:+2619961 MS.gene015821:CDS
CTGGATCGGCACCGGCGTAA+TGG 0.282706 4.2:+2619627 MS.gene015821:CDS
CAGCCCAGGCGGGTGTTTGA+TGG 0.285087 4.2:-2619607 None:intergenic
CGGGTGTTTGATGGGGTTAT+GGG 0.288686 4.2:-2619598 None:intergenic
TCCCTAAATCCTTCACAAAA+TGG 0.292923 4.2:-2620317 None:intergenic
CGGCGAGTGCGCCGGTGTTT+GGG 0.329981 4.2:-2619843 None:intergenic
GTCGAGGAGTGGTTGGGCTT+TGG 0.338068 4.2:-2619716 None:intergenic
CATTCTCACCCGCCACTTCA+TGG 0.353626 4.2:+2619564 MS.gene015821:CDS
TGGATCGGCACCGGCGTAAT+GGG 0.358966 4.2:+2619628 MS.gene015821:CDS
GTTTGATGGGGTTATGGGCT+CGG 0.365692 4.2:-2619593 None:intergenic
GGAGTGGTTGGGCTTTGGAG+GGG 0.366756 4.2:-2619711 None:intergenic
GAGTGGTTGGGCTTTGGAGG+GGG 0.366905 4.2:-2619710 None:intergenic
GAGGAGTGGTTGGGCTTTGG+AGG 0.376743 4.2:-2619713 None:intergenic
AGCCCAGGCGGGTGTTTGAT+GGG 0.383843 4.2:-2619606 None:intergenic
GCTCATGGTGAAGGTAATTT+GGG 0.387757 4.2:+2620420 MS.gene015821:CDS
GGGTATTGCTTTGCCTGGTT+TGG 0.390445 4.2:+2620365 MS.gene015821:CDS
ACAGCTTTATGTGTCTCTTA+AGG 0.398105 4.2:+2620398 MS.gene015821:CDS
GCCACGGTCTCCACCGGAAA+CGG 0.412055 4.2:-2619974 None:intergenic
CATCAAACACCCGCCTGGGC+TGG 0.416712 4.2:+2619608 MS.gene015821:CDS
AACCCCATCAAACACCCGCC+TGG 0.417289 4.2:+2619603 MS.gene015821:CDS
TGGGGTTATGGGCTCGGAGG+TGG 0.424426 4.2:-2619587 None:intergenic
ACTTGATGTAGGGTTGTATC+TGG 0.426394 4.2:+2620200 MS.gene015821:CDS
TCGAAATCGCTTGAGTTGTA+TGG 0.429219 4.2:+2620249 MS.gene015821:CDS
GCAATTTCGACGGGTGCGGC+TGG 0.432079 4.2:+2620225 MS.gene015821:CDS
CGCCGATGTCGAGGAGTGGT+TGG 0.432520 4.2:-2619723 None:intergenic
GGATTGTGATGCGACGGCGT+GGG 0.436919 4.2:-2619764 None:intergenic
CCACGGTCTCCACCGGAAAC+GGG 0.438644 4.2:-2619973 None:intergenic
AGGAGTGGTTGGGCTTTGGA+GGG 0.441675 4.2:-2619712 None:intergenic
TTCAACAATGGTAGGGTTAG+TGG 0.453377 4.2:+2620149 MS.gene015821:CDS
TGAGATGGGCGCACATGGAT+TGG 0.459719 4.2:-2619651 None:intergenic
TGCGCCCATCTCATCCGTTC+CGG 0.464896 4.2:+2619661 MS.gene015821:CDS
GAAGACGGTGAGGGTGTAAC+CGG 0.466194 4.2:-2619680 None:intergenic
GATTGTGATGCGACGGCGTG+GGG 0.468193 4.2:-2619763 None:intergenic
TACATGGGAACTAGCGGAAA+AGG 0.469512 4.2:+2620090 MS.gene015821:CDS
CACGATTGATTCATCTCCAC+CGG 0.470291 4.2:-2620025 None:intergenic
CGCCTGGGCTGGATCGGCAC+CGG 0.472143 4.2:+2619619 MS.gene015821:CDS
ACGATAGCTTCAACAATGGT+AGG 0.474726 4.2:+2620141 MS.gene015821:CDS
AACACCCGCCTGGGCTGGAT+CGG 0.475780 4.2:+2619613 MS.gene015821:CDS
GCCGATGTCGAGGAGTGGTT+GGG 0.478660 4.2:-2619722 None:intergenic
GGAGACCGTGGCGCAAAGAA+TGG 0.480378 4.2:+2619985 MS.gene015821:CDS
TATGTGTCTCTTAAGGCTCA+TGG 0.483295 4.2:+2620405 MS.gene015821:CDS
CGGAGGTGGCCATGAAGTGG+CGG 0.483903 4.2:-2619573 None:intergenic
TTGAAAGAATGTGAGAAAAT+GGG 0.490579 4.2:+2620345 MS.gene015821:CDS
GCACTCGCCGGTCTCAAATC+CGG 0.490972 4.2:+2619856 MS.gene015821:CDS
TGTTTCTCTTCCACCTTCTG+TGG 0.492236 4.2:+2620482 MS.gene015821:CDS
GTGCGCCGGTGTTTGGGTCG+AGG 0.495329 4.2:-2619837 None:intergenic
CTCCACCGGCGAAGATTGCT+AGG 0.501858 4.2:-2620011 None:intergenic
GCCCAGGCGGGTGTTTGATG+GGG 0.515820 4.2:-2619605 None:intergenic
ACCCCATCAAACACCCGCCT+GGG 0.517433 4.2:+2619604 MS.gene015821:CDS
CTTTGCGCCACGGTCTCCAC+CGG 0.519012 4.2:-2619980 None:intergenic
GGATGCAATTTCGACGGGTG+CGG 0.521188 4.2:+2620221 MS.gene015821:CDS
TGAGGAGGTGAGTGTGAGAG+TGG 0.521698 4.2:-2619543 None:intergenic
GGGTGCCATTCTTTGCGCCA+CGG 0.523853 4.2:-2619990 None:intergenic
TGTAACCGGAACGGATGAGA+TGG 0.525879 4.2:-2619666 None:intergenic
TTGTGATGCGACGGCGTGGG+GGG 0.527594 4.2:-2619761 None:intergenic
CGCCGGTGCCGATCCAGCCC+AGG 0.529107 4.2:-2619621 None:intergenic
GGTGTTTGGGTCGAGGAGGA+CGG 0.532059 4.2:-2619830 None:intergenic
TGAAGTGGCGGGTGAGAATG+AGG 0.533969 4.2:-2619561 None:intergenic
AAAATGGGTATTGCTTTGCC+TGG 0.535454 4.2:+2620360 MS.gene015821:CDS
GTATCTGGATGCAATTTCGA+CGG 0.537538 4.2:+2620215 MS.gene015821:CDS
ACGGATGAGATGGGCGCACA+TGG 0.540422 4.2:-2619656 None:intergenic
TTTGAGACCGGCGAGTGCGC+CGG 0.541946 4.2:-2619851 None:intergenic
GCTCGGAGGTGGCCATGAAG+TGG 0.545180 4.2:-2619576 None:intergenic
CGAGGGTGGCGCCGATGTCG+AGG 0.546061 4.2:-2619732 None:intergenic
AGGACGGATCGAACGTCGGA+GGG 0.546487 4.2:-2619814 None:intergenic
GATAACGATAGCTTCAACAA+TGG 0.552492 4.2:+2620137 MS.gene015821:CDS
TGAAGGTAATTTGGGTACTC+AGG 0.552977 4.2:+2620428 MS.gene015821:CDS
CACAAAGCGGGACTTGATGT+AGG 0.553732 4.2:+2620189 MS.gene015821:CDS
CCAAACACCGGCGCACTCGC+CGG 0.553830 4.2:+2619844 MS.gene015821:CDS
GGTGCCGATCCAGCCCAGGC+GGG 0.557386 4.2:-2619617 None:intergenic
GAGGACGGATCGAACGTCGG+AGG 0.559400 4.2:-2619815 None:intergenic
TCTCTTAAGGCTCATGGTGA+AGG 0.559680 4.2:+2620411 MS.gene015821:CDS
GGTGCGGTTGAAGACGGTGA+GGG 0.559737 4.2:-2619689 None:intergenic
GATGCCACCGGATTTGAGAC+CGG 0.562962 4.2:-2619863 None:intergenic
GCGATTTCGACGGCGAGGGA+AGG 0.563490 4.2:-2619910 None:intergenic
GGAGGTGGCCATGAAGTGGC+GGG 0.565150 4.2:-2619572 None:intergenic
TATCTGGATGCAATTTCGAC+GGG 0.565278 4.2:+2620216 MS.gene015821:CDS
CGGATTGTGATGCGACGGCG+TGG 0.568717 4.2:-2619765 None:intergenic
TGTTGAGCAAGTGCCAAACC+AGG 0.570957 4.2:-2620378 None:intergenic
GACGGCGAGGGAAGGATCTG+AGG 0.573970 4.2:-2619902 None:intergenic
ATGGTGTATGCACACAAAGC+GGG 0.574320 4.2:+2620177 MS.gene015821:CDS
TGCAGCGTCGGCGATTTCGA+CGG 0.575228 4.2:-2619920 None:intergenic
CAATGGTAGGGTTAGTGGAA+GGG 0.575951 4.2:+2620154 MS.gene015821:CDS
CCCGTTTCCGGTGGAGACCG+TGG 0.577236 4.2:+2619973 MS.gene015821:CDS
CATGTCAACGAGGATGCCAC+CGG 0.578295 4.2:-2619875 None:intergenic
ATCGACGCTCCCGTTTCCGG+TGG 0.581064 4.2:+2619964 MS.gene015821:CDS
ACAATGGTAGGGTTAGTGGA+AGG 0.583861 4.2:+2620153 MS.gene015821:CDS
TGATGCGGCGGTAGAGTGAA+CGG 0.584559 4.2:-2619519 None:intergenic
TGGTAAGAGAATTCTGAAGA+GGG 0.585420 4.2:+2620269 MS.gene015821:CDS
CATGGATTGGCCCATTACGC+CGG 0.585916 4.2:-2619638 None:intergenic
GGTAGGGTTAGTGGAAGGGA+TGG 0.586755 4.2:+2620158 MS.gene015821:CDS
CGGAAACGTAGGAATGGAAG+TGG 0.589829 4.2:-2619499 None:intergenic
GTAACCGGAACGGATGAGAT+GGG 0.590915 4.2:-2619665 None:intergenic
TGATTTCATACCACAGAAGG+TGG 0.591447 4.2:-2620492 None:intergenic
CGGTGCCGATCCAGCCCAGG+CGG 0.593100 4.2:-2619618 None:intergenic
CGATAGCTTCAACAATGGTA+GGG 0.594033 4.2:+2620142 MS.gene015821:CDS
GCCCAACCACTCCTCGACAT+CGG 0.595927 4.2:+2619721 MS.gene015821:CDS
ATTGTGATGCGACGGCGTGG+GGG 0.599351 4.2:-2619762 None:intergenic
GAATGATTTCATACCACAGA+AGG 0.605162 4.2:-2620495 None:intergenic
TTCCCCAATGATGAAAACAA+TGG 0.611373 4.2:-2620060 None:intergenic
ACAAAGCGGGACTTGATGTA+GGG 0.611508 4.2:+2620190 MS.gene015821:CDS
GTCCTCCTCGACCCAAACAC+CGG 0.611555 4.2:+2619832 MS.gene015821:CDS
AGTGAACGGAAACGTAGGAA+TGG 0.613244 4.2:-2619505 None:intergenic
GGCACCCTAGCAATCTTCGC+CGG 0.613387 4.2:+2620006 MS.gene015821:CDS
GTGGCGCCGATGTCGAGGAG+TGG 0.618486 4.2:-2619727 None:intergenic
GGGTGCGGTTGAAGACGGTG+AGG 0.619880 4.2:-2619690 None:intergenic
GTCGGCGATTTCGACGGCGA+GGG 0.623638 4.2:-2619914 None:intergenic
GGACGGATCGAACGTCGGAG+GGG 0.625313 4.2:-2619813 None:intergenic
CTCGCCGGTCTCAAATCCGG+TGG 0.625923 4.2:+2619859 MS.gene015821:CDS
ATGGTAAGAGAATTCTGAAG+AGG 0.627122 4.2:+2620268 MS.gene015821:CDS
CGTCGGCGATTTCGACGGCG+AGG 0.628317 4.2:-2619915 None:intergenic
GACGTCGGATTGTGATGCGA+CGG 0.628555 4.2:-2619770 None:intergenic
ACCCTAGCAATCTTCGCCGG+TGG 0.629709 4.2:+2620009 MS.gene015821:CDS
GAGTGTGAGAGTGGCTGATG+CGG 0.630259 4.2:-2619534 None:intergenic
ATTGGGGAAAGTGAACTACA+TGG 0.635633 4.2:+2620074 MS.gene015821:CDS
TGATGGGGTTATGGGCTCGG+AGG 0.636250 4.2:-2619590 None:intergenic
TGTGAGAGTGGCTGATGCGG+CGG 0.637898 4.2:-2619531 None:intergenic
GTCGGAGGGGTAACCAACGA+TGG 0.641353 4.2:-2619800 None:intergenic
CGCCGGTGTTTGGGTCGAGG+AGG 0.641635 4.2:-2619834 None:intergenic
GTGAACTACATGGGAACTAG+CGG 0.652338 4.2:+2620084 MS.gene015821:CDS
GGTAGAGTGAACGGAAACGT+AGG 0.661215 4.2:-2619510 None:intergenic
TCCACCGGCGAAGATTGCTA+GGG 0.664093 4.2:-2620010 None:intergenic
CGGTGAGGGTGTAACCGGAA+CGG 0.665734 4.2:-2619675 None:intergenic
GATGGTGTATGCACACAAAG+CGG 0.671363 4.2:+2620176 MS.gene015821:CDS
TTGGGGAAAGTGAACTACAT+GGG 0.676398 4.2:+2620075 MS.gene015821:CDS
GAGGAGGACGGATCGAACGT+CGG 0.678301 4.2:-2619818 None:intergenic
AACGATGGAGAAAACGACGT+CGG 0.717582 4.2:-2619785 None:intergenic
CTGAGGTTGTCATGTCAACG+AGG 0.717734 4.2:-2619885 None:intergenic
AGTGGCGGGTGAGAATGAGG+AGG 0.762010 4.2:-2619558 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTGAAAGAATGTGAGAAAAT+GGG + chr4.2:2620345-2620364 MS.gene015821:CDS 25.0%
! TTTGAAAGAATGTGAGAAAA+TGG + chr4.2:2620344-2620363 MS.gene015821:CDS 25.0%
! AACCATTTTGTGAAGGATTT+AGG + chr4.2:2620315-2620334 MS.gene015821:CDS 30.0%
! ACCATTTTGTGAAGGATTTA+GGG + chr4.2:2620316-2620335 MS.gene015821:CDS 30.0%
! TCTCCATTGTTTTCATCATT+GGG + chr4.2:2620057-2620076 MS.gene015821:CDS 30.0%
! TTATGTGAACCATTTTGTGA+AGG + chr4.2:2620308-2620327 MS.gene015821:CDS 30.0%
! TTCTCCATTGTTTTCATCAT+TGG + chr4.2:2620056-2620075 MS.gene015821:CDS 30.0%
GATAACGATAGCTTCAACAA+TGG + chr4.2:2620137-2620156 MS.gene015821:CDS 35.0%
TCCCTAAATCCTTCACAAAA+TGG - chr4.2:2620320-2620339 None:intergenic 35.0%
TTCCCCAATGATGAAAACAA+TGG - chr4.2:2620063-2620082 None:intergenic 35.0%
! ACAGCTTTATGTGTCTCTTA+AGG + chr4.2:2620398-2620417 MS.gene015821:CDS 35.0%
! ATGGTAAGAGAATTCTGAAG+AGG + chr4.2:2620268-2620287 MS.gene015821:CDS 35.0%
! CTCCATTGTTTTCATCATTG+GGG + chr4.2:2620058-2620077 MS.gene015821:CDS 35.0%
! TGGTAAGAGAATTCTGAAGA+GGG + chr4.2:2620269-2620288 MS.gene015821:CDS 35.0%
ACGATAGCTTCAACAATGGT+AGG + chr4.2:2620141-2620160 MS.gene015821:CDS 40.0%
ACTTGATGTAGGGTTGTATC+TGG + chr4.2:2620200-2620219 MS.gene015821:CDS 40.0%
ATTGGGGAAAGTGAACTACA+TGG + chr4.2:2620074-2620093 MS.gene015821:CDS 40.0%
CGATAGCTTCAACAATGGTA+GGG + chr4.2:2620142-2620161 MS.gene015821:CDS 40.0%
GCTCATGGTGAAGGTAATTT+GGG + chr4.2:2620420-2620439 MS.gene015821:CDS 40.0%
GTATCTGGATGCAATTTCGA+CGG + chr4.2:2620215-2620234 MS.gene015821:CDS 40.0%
TATCTGGATGCAATTTCGAC+GGG + chr4.2:2620216-2620235 MS.gene015821:CDS 40.0%
TATGTGTCTCTTAAGGCTCA+TGG + chr4.2:2620405-2620424 MS.gene015821:CDS 40.0%
TGAAGGTAATTTGGGTACTC+AGG + chr4.2:2620428-2620447 MS.gene015821:CDS 40.0%
TTCAACAATGGTAGGGTTAG+TGG + chr4.2:2620149-2620168 MS.gene015821:CDS 40.0%
TTGGGGAAAGTGAACTACAT+GGG + chr4.2:2620075-2620094 MS.gene015821:CDS 40.0%
! AAAATGGGTATTGCTTTGCC+TGG + chr4.2:2620360-2620379 MS.gene015821:CDS 40.0%
! TCGAAATCGCTTGAGTTGTA+TGG + chr4.2:2620249-2620268 MS.gene015821:CDS 40.0%
!! GTTTTTAACTGATGCAGCGT+CGG - chr4.2:2619935-2619954 None:intergenic 40.0%
!!! TGAAGAGGGATTTTGAAGCA+GGG + chr4.2:2620283-2620302 MS.gene015821:CDS 40.0%
AACGATGGAGAAAACGACGT+CGG - chr4.2:2619788-2619807 None:intergenic 45.0%
ACAAAGCGGGACTTGATGTA+GGG + chr4.2:2620190-2620209 MS.gene015821:CDS 45.0%
AGTGAACGGAAACGTAGGAA+TGG - chr4.2:2619508-2619527 None:intergenic 45.0%
ATGGTGTATGCACACAAAGC+GGG + chr4.2:2620177-2620196 MS.gene015821:CDS 45.0%
GATGGTGTATGCACACAAAG+CGG + chr4.2:2620176-2620195 MS.gene015821:CDS 45.0%
GGCTCATGGTGAAGGTAATT+TGG + chr4.2:2620419-2620438 MS.gene015821:CDS 45.0%
GTGAACTACATGGGAACTAG+CGG + chr4.2:2620084-2620103 MS.gene015821:CDS 45.0%
TACATGGGAACTAGCGGAAA+AGG + chr4.2:2620090-2620109 MS.gene015821:CDS 45.0%
TCTCTTAAGGCTCATGGTGA+AGG + chr4.2:2620411-2620430 MS.gene015821:CDS 45.0%
TGTTTCTCTTCCACCTTCTG+TGG + chr4.2:2620482-2620501 MS.gene015821:CDS 45.0%
! ACAATGGTAGGGTTAGTGGA+AGG + chr4.2:2620153-2620172 MS.gene015821:CDS 45.0%
! CAATGGTAGGGTTAGTGGAA+GGG + chr4.2:2620154-2620173 MS.gene015821:CDS 45.0%
! CACGATTGATTCATCTCCAC+CGG - chr4.2:2620028-2620047 None:intergenic 45.0%
!! GGGTACTCAGGCTCTTATTT+TGG + chr4.2:2620440-2620459 MS.gene015821:CDS 45.0%
!!! CTGAAGAGGGATTTTGAAGC+AGG + chr4.2:2620282-2620301 MS.gene015821:CDS 45.0%
CACAAAGCGGGACTTGATGT+AGG + chr4.2:2620189-2620208 MS.gene015821:CDS 50.0%
CGGAAACGTAGGAATGGAAG+TGG - chr4.2:2619502-2619521 None:intergenic 50.0%
CGGGTGTTTGATGGGGTTAT+GGG - chr4.2:2619601-2619620 None:intergenic 50.0%
CTGAGGTTGTCATGTCAACG+AGG - chr4.2:2619888-2619907 None:intergenic 50.0%
GGTAGAGTGAACGGAAACGT+AGG - chr4.2:2619513-2619532 None:intergenic 50.0%
GTAACCGGAACGGATGAGAT+GGG - chr4.2:2619668-2619687 None:intergenic 50.0%
GTTTGATGGGGTTATGGGCT+CGG - chr4.2:2619596-2619615 None:intergenic 50.0%
TGTAACCGGAACGGATGAGA+TGG - chr4.2:2619669-2619688 None:intergenic 50.0%
TGTTGAGCAAGTGCCAAACC+AGG - chr4.2:2620381-2620400 None:intergenic 50.0%
! GGGTATTGCTTTGCCTGGTT+TGG + chr4.2:2620365-2620384 MS.gene015821:CDS 50.0%
!! GACGTCGTTTTCTCCATCGT+TGG + chr4.2:2619787-2619806 MS.gene015821:CDS 50.0%
CATGTCAACGAGGATGCCAC+CGG - chr4.2:2619878-2619897 None:intergenic 55.0%
CATTCTCACCCGCCACTTCA+TGG + chr4.2:2619564-2619583 MS.gene015821:CDS 55.0%
GACGTCGGATTGTGATGCGA+CGG - chr4.2:2619773-2619792 None:intergenic 55.0%
GAGTGTGAGAGTGGCTGATG+CGG - chr4.2:2619537-2619556 None:intergenic 55.0%
GCGGGTGTTTGATGGGGTTA+TGG - chr4.2:2619602-2619621 None:intergenic 55.0%
GGATGCAATTTCGACGGGTG+CGG + chr4.2:2620221-2620240 MS.gene015821:CDS 55.0%
TCAATCGACGCTCCCGTTTC+CGG + chr4.2:2619961-2619980 MS.gene015821:CDS 55.0%
TCCACCGGCGAAGATTGCTA+GGG - chr4.2:2620013-2620032 None:intergenic 55.0%
TGAGATGGGCGCACATGGAT+TGG - chr4.2:2619654-2619673 None:intergenic 55.0%
TGAGGAGGTGAGTGTGAGAG+TGG - chr4.2:2619546-2619565 None:intergenic 55.0%
TGATGCGGCGGTAGAGTGAA+CGG - chr4.2:2619522-2619541 None:intergenic 55.0%
! GAAGACGGTGAGGGTGTAAC+CGG - chr4.2:2619683-2619702 None:intergenic 55.0%
! GATGCCACCGGATTTGAGAC+CGG - chr4.2:2619866-2619885 None:intergenic 55.0%
! GGTAGGGTTAGTGGAAGGGA+TGG + chr4.2:2620158-2620177 MS.gene015821:CDS 55.0%
!! AGGAGTGGTTGGGCTTTGGA+GGG - chr4.2:2619715-2619734 None:intergenic 55.0%
!! CATGGATTGGCCCATTACGC+CGG - chr4.2:2619641-2619660 None:intergenic 55.0%
!! TGAAGTGGCGGGTGAGAATG+AGG - chr4.2:2619564-2619583 None:intergenic 55.0%
AACCCCATCAAACACCCGCC+TGG + chr4.2:2619603-2619622 MS.gene015821:CDS 60.0%
ACCCCATCAAACACCCGCCT+GGG + chr4.2:2619604-2619623 MS.gene015821:CDS 60.0%
ACCCTAGCAATCTTCGCCGG+TGG + chr4.2:2620009-2620028 MS.gene015821:CDS 60.0%
ACGGATGAGATGGGCGCACA+TGG - chr4.2:2619659-2619678 None:intergenic 60.0%
AGCCCAGGCGGGTGTTTGAT+GGG - chr4.2:2619609-2619628 None:intergenic 60.0%
AGGACGGATCGAACGTCGGA+GGG - chr4.2:2619817-2619836 None:intergenic 60.0%
ATTGTGATGCGACGGCGTGG+GGG - chr4.2:2619765-2619784 None:intergenic 60.0%
CTCCACCGGCGAAGATTGCT+AGG - chr4.2:2620014-2620033 None:intergenic 60.0%
GAGGAGGACGGATCGAACGT+CGG - chr4.2:2619821-2619840 None:intergenic 60.0%
GATTGTGATGCGACGGCGTG+GGG - chr4.2:2619766-2619785 None:intergenic 60.0%
GCACTCGCCGGTCTCAAATC+CGG + chr4.2:2619856-2619875 MS.gene015821:CDS 60.0%
GCCCAACCACTCCTCGACAT+CGG + chr4.2:2619721-2619740 MS.gene015821:CDS 60.0%
GCCGATGTCGAGGAGTGGTT+GGG - chr4.2:2619725-2619744 None:intergenic 60.0%
GGAGACCGTGGCGCAAAGAA+TGG + chr4.2:2619985-2620004 MS.gene015821:CDS 60.0%
GGATTGTGATGCGACGGCGT+GGG - chr4.2:2619767-2619786 None:intergenic 60.0%
GGCACCCTAGCAATCTTCGC+CGG + chr4.2:2620006-2620025 MS.gene015821:CDS 60.0%
GGTGCGGTTGAAGACGGTGA+GGG - chr4.2:2619692-2619711 None:intergenic 60.0%
GGTGTTTGGGTCGAGGAGGA+CGG - chr4.2:2619833-2619852 None:intergenic 60.0%
GTCCTCCTCGACCCAAACAC+CGG + chr4.2:2619832-2619851 MS.gene015821:CDS 60.0%
GTCGAGGAGTGGTTGGGCTT+TGG - chr4.2:2619719-2619738 None:intergenic 60.0%
GTCGGAGGGGTAACCAACGA+TGG - chr4.2:2619803-2619822 None:intergenic 60.0%
TGATGGGGTTATGGGCTCGG+AGG - chr4.2:2619593-2619612 None:intergenic 60.0%
TGCGCCCATCTCATCCGTTC+CGG + chr4.2:2619661-2619680 MS.gene015821:CDS 60.0%
TGTGAGAGTGGCTGATGCGG+CGG - chr4.2:2619534-2619553 None:intergenic 60.0%
! CGGTGAGGGTGTAACCGGAA+CGG - chr4.2:2619678-2619697 None:intergenic 60.0%
! GAGGAGTGGTTGGGCTTTGG+AGG - chr4.2:2619716-2619735 None:intergenic 60.0%
!! AGTGGCGGGTGAGAATGAGG+AGG - chr4.2:2619561-2619580 None:intergenic 60.0%
!! GAGTGGTTGGGCTTTGGAGG+GGG - chr4.2:2619713-2619732 None:intergenic 60.0%
!! GGAGTGGTTGGGCTTTGGAG+GGG - chr4.2:2619714-2619733 None:intergenic 60.0%
!! GGGTGCCATTCTTTGCGCCA+CGG - chr4.2:2619993-2620012 None:intergenic 60.0%
!! TGCAGCGTCGGCGATTTCGA+CGG - chr4.2:2619923-2619942 None:intergenic 60.0%
!! TGGATCGGCACCGGCGTAAT+GGG + chr4.2:2619628-2619647 MS.gene015821:CDS 60.0%
ATCGACGCTCCCGTTTCCGG+TGG + chr4.2:2619964-2619983 MS.gene015821:CDS 65.0%
CAGCCCAGGCGGGTGTTTGA+TGG - chr4.2:2619610-2619629 None:intergenic 65.0%
CATCAAACACCCGCCTGGGC+TGG + chr4.2:2619608-2619627 MS.gene015821:CDS 65.0%
CCACGGTCTCCACCGGAAAC+GGG - chr4.2:2619976-2619995 None:intergenic 65.0%
CGCCGATGTCGAGGAGTGGT+TGG - chr4.2:2619726-2619745 None:intergenic 65.0%
CGGAGGTGGCCATGAAGTGG+CGG - chr4.2:2619576-2619595 None:intergenic 65.0%
CGGATTGTGATGCGACGGCG+TGG - chr4.2:2619768-2619787 None:intergenic 65.0%
CTCGCCGGTCTCAAATCCGG+TGG + chr4.2:2619859-2619878 MS.gene015821:CDS 65.0%
CTTTGCGCCACGGTCTCCAC+CGG - chr4.2:2619983-2620002 None:intergenic 65.0%
GACGGCGAGGGAAGGATCTG+AGG - chr4.2:2619905-2619924 None:intergenic 65.0%
GAGGACGGATCGAACGTCGG+AGG - chr4.2:2619818-2619837 None:intergenic 65.0%
GCAATTTCGACGGGTGCGGC+TGG + chr4.2:2620225-2620244 MS.gene015821:CDS 65.0%
GCCACGGTCTCCACCGGAAA+CGG - chr4.2:2619977-2619996 None:intergenic 65.0%
GCCCAGGCGGGTGTTTGATG+GGG - chr4.2:2619608-2619627 None:intergenic 65.0%
GCGATTTCGACGGCGAGGGA+AGG - chr4.2:2619913-2619932 None:intergenic 65.0%
GCTCGGAGGTGGCCATGAAG+TGG - chr4.2:2619579-2619598 None:intergenic 65.0%
GGACGGATCGAACGTCGGAG+GGG - chr4.2:2619816-2619835 None:intergenic 65.0%
GGAGGTGGCCATGAAGTGGC+GGG - chr4.2:2619575-2619594 None:intergenic 65.0%
GGGTGCGGTTGAAGACGGTG+AGG - chr4.2:2619693-2619712 None:intergenic 65.0%
GTCGGCGATTTCGACGGCGA+GGG - chr4.2:2619917-2619936 None:intergenic 65.0%
TGGGGTTATGGGCTCGGAGG+TGG - chr4.2:2619590-2619609 None:intergenic 65.0%
TTGTGATGCGACGGCGTGGG+GGG - chr4.2:2619764-2619783 None:intergenic 65.0%
! AACACCCGCCTGGGCTGGAT+CGG + chr4.2:2619613-2619632 MS.gene015821:CDS 65.0%
! GGAGGGGGTGCGGTTGAAGA+CGG - chr4.2:2619698-2619717 None:intergenic 65.0%
! TTTGAGACCGGCGAGTGCGC+CGG - chr4.2:2619854-2619873 None:intergenic 65.0%
!! CTGGATCGGCACCGGCGTAA+TGG + chr4.2:2619627-2619646 MS.gene015821:CDS 65.0%
!! GTTGGGCTTTGGAGGGGGTG+CGG - chr4.2:2619708-2619727 None:intergenic 65.0%
CCAAACACCGGCGCACTCGC+CGG + chr4.2:2619844-2619863 MS.gene015821:CDS 70.0%
CCCGTTTCCGGTGGAGACCG+TGG + chr4.2:2619973-2619992 MS.gene015821:CDS 70.0%
CGCCGGTGTTTGGGTCGAGG+AGG - chr4.2:2619837-2619856 None:intergenic 70.0%
CGTCGGCGATTTCGACGGCG+AGG - chr4.2:2619918-2619937 None:intergenic 70.0%
! CGGCGAGTGCGCCGGTGTTT+GGG - chr4.2:2619846-2619865 None:intergenic 70.0%
! GTGCGCCGGTGTTTGGGTCG+AGG - chr4.2:2619840-2619859 None:intergenic 70.0%
!! GTGGCGCCGATGTCGAGGAG+TGG - chr4.2:2619730-2619749 None:intergenic 70.0%
GGCGTGGGGGGAAGTGGCGA+GGG - chr4.2:2619752-2619771 None:intergenic 75.0%
GTGGGGGGAAGTGGCGAGGG+TGG - chr4.2:2619749-2619768 None:intergenic 75.0%
TGCGACGGCGTGGGGGGAAG+TGG - chr4.2:2619758-2619777 None:intergenic 75.0%
! CCGGCGAGTGCGCCGGTGTT+TGG - chr4.2:2619847-2619866 None:intergenic 75.0%
! CGCCTGGGCTGGATCGGCAC+CGG + chr4.2:2619619-2619638 MS.gene015821:CDS 75.0%
!! CGAGGGTGGCGCCGATGTCG+AGG - chr4.2:2619735-2619754 None:intergenic 75.0%
!! CGGTGCCGATCCAGCCCAGG+CGG - chr4.2:2619621-2619640 None:intergenic 75.0%
!! GGTGCCGATCCAGCCCAGGC+GGG - chr4.2:2619620-2619639 None:intergenic 75.0%
! CGGCGTGGGGGGAAGTGGCG+AGG - chr4.2:2619753-2619772 None:intergenic 80.0%
!!! CGCCGGTGCCGATCCAGCCC+AGG - chr4.2:2619624-2619643 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 2619496 2620509 2619496 ID=MS.gene015821
chr4.2 mRNA 2619496 2620509 2619496 ID=MS.gene015821.t1;Parent=MS.gene015821
chr4.2 exon 2619496 2620509 2619496 ID=MS.gene015821.t1.exon1;Parent=MS.gene015821.t1
chr4.2 CDS 2619496 2620509 2619496 ID=cds.MS.gene015821.t1;Parent=MS.gene015821.t1
Gene Sequence

>MS.gene015821

ATGCCACTTCCATTCCTACGTTTCCGTTCACTCTACCGCCGCATCAGCCACTCTCACACTCACCTCCTCATTCTCACCCGCCACTTCATGGCCACCTCCGAGCCCATAACCCCATCAAACACCCGCCTGGGCTGGATCGGCACCGGCGTAATGGGCCAATCCATGTGCGCCCATCTCATCCGTTCCGGTTACACCCTCACCGTCTTCAACCGCACCCCCTCCAAAGCCCAACCACTCCTCGACATCGGCGCCACCCTCGCCACTTCCCCCCACGCCGTCGCATCACAATCCGACGTCGTTTTCTCCATCGTTGGTTACCCCTCCGACGTTCGATCCGTCCTCCTCGACCCAAACACCGGCGCACTCGCCGGTCTCAAATCCGGTGGCATCCTCGTTGACATGACAACCTCAGATCCTTCCCTCGCCGTCGAAATCGCCGACGCTGCATCAGTTAAAAACTGTCACTCAATCGACGCTCCCGTTTCCGGTGGAGACCGTGGCGCAAAGAATGGCACCCTAGCAATCTTCGCCGGTGGAGATGAATCAATCGTGAAAAAACTTTCTCCATTGTTTTCATCATTGGGGAAAGTGAACTACATGGGAACTAGCGGAAAAGGTCAATTCGCGAAGCTAGCGAATCAGATAACGATAGCTTCAACAATGGTAGGGTTAGTGGAAGGGATGGTGTATGCACACAAAGCGGGACTTGATGTAGGGTTGTATCTGGATGCAATTTCGACGGGTGCGGCTGGTTCGAAATCGCTTGAGTTGTATGGTAAGAGAATTCTGAAGAGGGATTTTGAAGCAGGGTTTTATGTGAACCATTTTGTGAAGGATTTAGGGATTTGTTTGAAAGAATGTGAGAAAATGGGTATTGCTTTGCCTGGTTTGGCACTTGCTCAACAGCTTTATGTGTCTCTTAAGGCTCATGGTGAAGGTAATTTGGGTACTCAGGCTCTTATTTTGGTTCTTGAGCGACTCAACAATGTTTCTCTTCCACCTTCTGTGGTATGA
Protein sequence

>MS.gene015821.t1

MPLPFLRFRSLYRRISHSHTHLLILTRHFMATSEPITPSNTRLGWIGTGVMGQSMCAHLIRSGYTLTVFNRTPSKAQPLLDIGATLATSPHAVASQSDVVFSIVGYPSDVRSVLLDPNTGALAGLKSGGILVDMTTSDPSLAVEIADAASVKNCHSIDAPVSGGDRGAKNGTLAIFAGGDESIVKKLSPLFSSLGKVNYMGTSGKGQFAKLANQITIASTMVGLVEGMVYAHKAGLDVGLYLDAISTGAAGSKSLELYGKRILKRDFEAGFYVNHFVKDLGICLKECEKMGIALPGLALAQQLYVSLKAHGEGNLGTQALILVLERLNNVSLPPSVV