AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016921

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016921.t1 MTR_5g088720 98.769 325 4 0 1 325 47 371 0.0 672
MS.gene016921.t1 MTR_5g088720 98.769 325 4 0 1 325 6 330 0.0 667
MS.gene016921.t1 MTR_5g088720 98.769 325 4 0 1 325 1 325 0.0 667
MS.gene016921.t1 MTR_0067s0100 61.409 298 95 6 1 297 2 280 1.75e-120 348
MS.gene016921.t1 MTR_1g051105 39.759 332 112 9 2 324 4 256 8.60e-71 221
MS.gene016921.t1 MTR_3g072630 34.630 257 140 5 7 245 20 266 1.11e-41 147
MS.gene016921.t1 MTR_3g072630 34.630 257 140 5 7 245 20 266 1.75e-41 147
MS.gene016921.t1 MTR_3g068005 35.769 260 133 7 1 245 1 241 1.69e-38 141
MS.gene016921.t1 MTR_6g079660 51.969 127 52 4 123 245 159 280 3.37e-37 137
MS.gene016921.t1 MTR_4g064570 35.341 249 119 9 6 245 11 226 8.06e-37 135
MS.gene016921.t1 MTR_5g080350 32.852 277 146 9 5 263 2 256 3.33e-36 134
MS.gene016921.t1 MTR_2g097620 52.000 100 43 1 151 245 135 234 2.29e-32 123
MS.gene016921.t1 MTR_3g083480 43.312 157 64 6 147 288 187 333 3.02e-32 123
MS.gene016921.t1 MTR_1g114320 31.387 274 141 10 8 264 14 257 4.20e-32 122
MS.gene016921.t1 MTR_7g074170 41.228 114 64 2 144 254 286 399 1.04e-25 107
MS.gene016921.t1 MTR_2g007340 51.685 89 42 1 157 244 157 245 2.03e-23 98.6
MS.gene016921.t1 MTR_1g114620 41.463 123 58 3 126 245 198 309 2.88e-23 98.6
MS.gene016921.t1 MTR_6g032960 36.986 146 79 5 147 288 184 320 5.26e-21 92.0
MS.gene016921.t1 MTR_5g041310 43.956 91 48 1 155 245 73 160 2.46e-19 85.5
MS.gene016921.t1 MTR_5g041330 45.055 91 47 1 155 245 63 150 3.37e-19 85.1
MS.gene016921.t1 MTR_4g110100 35.238 105 65 2 144 245 301 405 3.17e-17 82.4
MS.gene016921.t1 MTR_1g014710 36.957 92 53 2 158 244 186 277 1.67e-16 79.3
MS.gene016921.t1 MTR_3g088080 39.437 71 41 1 175 245 15 83 8.89e-16 72.0
MS.gene016921.t1 MTR_5g058530 49.153 59 29 1 187 244 210 268 6.11e-15 74.7
MS.gene016921.t1 MTR_6g016350 36.000 100 61 2 161 257 269 368 4.54e-14 72.8
MS.gene016921.t1 MTR_2g061790 54.762 42 19 0 200 241 109 150 5.06e-12 65.1
MS.gene016921.t1 MTR_4g106960 36.667 90 52 2 175 260 150 238 1.64e-11 64.7
MS.gene016921.t1 MTR_4g106960 36.667 90 52 2 175 260 150 238 1.90e-11 64.3
MS.gene016921.t1 MTR_4g106960 36.667 90 52 2 175 260 207 295 2.11e-11 64.7
MS.gene016921.t1 MTR_4g106960 36.667 90 52 2 175 260 207 295 2.47e-11 64.3
MS.gene016921.t1 MTR_5g022920 36.250 80 47 2 169 244 155 234 3.13e-11 62.8
MS.gene016921.t1 MTR_4g108830 36.508 63 39 1 180 241 126 188 6.85e-11 61.2
MS.gene016921.t1 MTR_4g108830 36.508 63 39 1 180 241 154 216 8.94e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016921.t1 AT2G40830 46.686 347 139 10 2 324 3 327 4.37e-81 249
MS.gene016921.t1 AT2G40830 46.686 347 139 10 2 324 3 327 4.37e-81 249
MS.gene016921.t1 AT2G40830 46.686 347 139 10 2 324 3 327 4.37e-81 249
MS.gene016921.t1 AT2G40830 46.686 347 139 10 2 324 3 327 4.37e-81 249
MS.gene016921.t1 AT3G56580 48.387 310 100 12 6 297 7 274 1.62e-69 219
MS.gene016921.t1 AT3G56580 48.387 310 100 12 6 297 7 274 1.62e-69 219
MS.gene016921.t1 AT3G56580 48.387 310 100 12 6 297 7 274 1.62e-69 219
MS.gene016921.t1 AT3G19950 35.039 254 135 8 8 245 22 261 2.82e-43 152
MS.gene016921.t1 AT3G19950 34.646 254 136 8 8 245 80 319 6.08e-43 152
MS.gene016921.t1 AT3G19950 34.646 254 136 8 8 245 80 319 6.08e-43 152
MS.gene016921.t1 AT1G60360 34.202 307 156 15 7 290 24 307 1.64e-40 144
MS.gene016921.t1 AT3G10815 50.000 142 42 4 158 296 78 193 2.85e-39 137
MS.gene016921.t1 AT1G55530 28.521 284 168 6 7 262 9 285 2.58e-35 131
MS.gene016921.t1 AT1G55530 28.521 284 168 6 7 262 9 285 2.58e-35 131
MS.gene016921.t1 AT5G56340 40.909 176 77 6 133 288 195 363 2.29e-34 130
MS.gene016921.t1 AT1G55530 29.231 260 164 7 7 262 9 252 2.12e-33 125
MS.gene016921.t1 AT5G59550 45.378 119 58 1 151 262 143 261 6.68e-30 118
MS.gene016921.t1 AT4G26400 40.972 144 75 3 149 287 193 331 7.23e-30 117
MS.gene016921.t1 AT4G26400 40.972 144 75 3 149 287 193 331 7.23e-30 117
MS.gene016921.t1 AT2G39720 34.074 270 133 11 1 247 1 248 1.29e-29 117
MS.gene016921.t1 AT2G39720 34.074 270 133 11 1 247 1 248 1.29e-29 117
MS.gene016921.t1 AT3G46620 50.000 102 44 1 151 245 159 260 1.42e-29 117
MS.gene016921.t1 AT5G59550 45.378 119 58 1 151 262 248 366 1.65e-29 118
MS.gene016921.t1 AT3G13430 47.059 102 47 2 147 245 173 270 7.81e-28 110
MS.gene016921.t1 AT3G13430 47.059 102 47 2 147 245 173 270 7.81e-28 110
MS.gene016921.t1 AT3G13430 47.059 102 47 2 147 245 173 270 7.81e-28 110
MS.gene016921.t1 AT3G13430 47.059 102 47 2 147 245 173 270 7.81e-28 110
MS.gene016921.t1 AT5G01980 43.363 113 62 1 144 254 292 404 2.94e-26 108
MS.gene016921.t1 AT1G68180 39.041 146 80 3 144 289 82 218 2.31e-25 102
MS.gene016921.t1 AT3G60080 50.602 83 33 1 181 255 142 224 4.10e-20 89.4
MS.gene016921.t1 AT5G20910 35.772 123 66 4 138 249 159 279 4.31e-19 86.7
MS.gene016921.t1 AT3G02340 41.837 98 55 2 171 267 305 401 5.82e-19 87.4
MS.gene016921.t1 AT3G02340 41.837 98 55 2 171 267 305 401 5.82e-19 87.4
MS.gene016921.t1 AT2G44330 38.655 119 63 3 133 245 27 141 2.50e-18 81.6
MS.gene016921.t1 AT5G15820 44.737 76 41 1 171 245 261 336 2.77e-18 84.7
MS.gene016921.t1 AT1G26800 40.860 93 51 1 156 248 73 161 4.34e-18 81.6
MS.gene016921.t1 AT5G60820 35.294 136 83 2 114 245 283 417 1.92e-17 82.8
MS.gene016921.t1 AT5G08139 36.752 117 66 5 147 258 255 368 6.80e-17 80.9
MS.gene016921.t1 AT5G64920 40.000 105 56 2 147 244 197 301 5.07e-16 78.2
MS.gene016921.t1 AT1G14200 36.842 76 48 0 170 245 79 154 2.76e-15 73.2
MS.gene016921.t1 AT3G58720 45.070 71 38 1 197 266 141 211 7.40e-12 64.7
MS.gene016921.t1 AT3G58720 45.070 71 38 1 197 266 161 231 8.97e-12 64.7
MS.gene016921.t1 AT3G58720 45.070 71 38 1 197 266 169 239 1.20e-11 64.7
MS.gene016921.t1 AT5G02750 46.154 52 28 0 200 251 214 265 2.10e-11 63.9
MS.gene016921.t1 AT4G10150 30.928 97 65 2 182 276 93 189 2.38e-11 63.2
MS.gene016921.t1 AT3G58720 45.070 71 38 1 197 266 169 239 3.11e-11 63.9
MS.gene016921.t1 AT3G63530 32.609 92 53 3 159 244 153 241 9.84e-11 61.6
MS.gene016921.t1 AT3G63530 32.609 92 53 3 159 244 153 241 9.84e-11 61.6

Find 96 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACAATAGGTTTGGGCTTA+TGG 0.144953 5.1:+70251211 MS.gene016921:CDS
TATAACGTGTTCCATTATTC+TGG 0.271470 5.1:-70251922 None:intergenic
TCCCAGTTCCTGAACAGGGC+TGG 0.287756 5.1:+70251312 MS.gene016921:CDS
GGCTGGGGCGTTTATAGTTC+TGG 0.293414 5.1:+70251329 MS.gene016921:CDS
CTCCCTCTAACATCAAAGTT+TGG 0.296005 5.1:-70251278 None:intergenic
GTATTTCATGGTCAAGTTCC+TGG 0.296498 5.1:+70251362 MS.gene016921:CDS
CCCAGTTCCTGAACAGGGCT+GGG 0.305104 5.1:+70251313 MS.gene016921:CDS
TTCTGTCCCAGTTCCTGAAC+AGG 0.322724 5.1:+70251307 MS.gene016921:CDS
ACCAATGCTGACCAGAATAA+TGG 0.327570 5.1:+70251911 MS.gene016921:CDS
GGATTTCTCCTTCCATTGTT+AGG 0.333484 5.1:-70251809 None:intergenic
AATAATGGAACACGTTATAA+TGG 0.335853 5.1:+70251926 MS.gene016921:CDS
TTGATGCAATGCCTACTATT+AGG 0.339004 5.1:+70251531 MS.gene016921:CDS
CCCCAGCCCTGTTCAGGAAC+TGG 0.343591 5.1:-70251314 None:intergenic
TACATTCAGAATGGTAAATA+TGG 0.350279 5.1:-70251646 None:intergenic
GGTCCTAGGCGTGGCGATTT+CGG 0.351499 5.1:+70251416 MS.gene016921:CDS
TAAACGCCCCAGCCCTGTTC+AGG 0.367352 5.1:-70251320 None:intergenic
AGAGGTTCTGACAATAGGTT+TGG 0.376287 5.1:+70251203 MS.gene016921:CDS
CACCCAAACTTTGATGTTAG+AGG 0.383978 5.1:+70251275 MS.gene016921:CDS
TCTCTCCCTTCAAGCACAAT+TGG 0.421897 5.1:-70251032 None:intergenic
TCCCTCTAACATCAAAGTTT+GGG 0.425458 5.1:-70251277 None:intergenic
TCCATTATTCTGGTCAGCAT+TGG 0.433872 5.1:-70251912 None:intergenic
GAAATGGTAGCTCAAGGGGC+AGG 0.434859 5.1:+70251774 MS.gene016921:CDS
CCACCTCTCGGGCTTCCATT+AGG 0.440593 5.1:-70251395 None:intergenic
CCTGGACTAACCTTGCCTAA+TGG 0.441564 5.1:+70251380 MS.gene016921:CDS
TCAATTGAAGATCGTGCAGC+TGG 0.442427 5.1:-70251512 None:intergenic
CGAAGCAACAGTGGTGGAAA+TGG 0.442575 5.1:+70251758 MS.gene016921:CDS
TGGACCACGCCGATCATTCA+TGG 0.450347 5.1:-70251486 None:intergenic
CCCAGCCCTGTTCAGGAACT+GGG 0.453761 5.1:-70251313 None:intergenic
ATTCTGAATGTATTGTTCCT+TGG 0.454347 5.1:+70251657 MS.gene016921:CDS
GGCACAGAGGTTCTGACAAT+AGG 0.463327 5.1:+70251198 MS.gene016921:CDS
CACGCCTAGGACCACCTCTC+GGG 0.474492 5.1:-70251406 None:intergenic
TTCATGAGGCATAGAATGGC+TGG 0.476150 5.1:+70251248 MS.gene016921:CDS
GGTAGCTCAACACGGCAAAC+AGG 0.476761 5.1:-70251701 None:intergenic
TGAATGTATTGTTCCTTGGT+TGG 0.476923 5.1:+70251661 MS.gene016921:CDS
GAGGTTCTGACAATAGGTTT+GGG 0.480899 5.1:+70251204 MS.gene016921:CDS
CCACGCCTAGGACCACCTCT+CGG 0.482101 5.1:-70251407 None:intergenic
TTCTCCTTCCATTGTTAGGA+CGG 0.494449 5.1:-70251805 None:intergenic
GAAGCCCGAGAGGTGGTCCT+AGG 0.494733 5.1:+70251402 MS.gene016921:CDS
ATGGTCACATGGCATCTCTC+TGG 0.495307 5.1:-70251627 None:intergenic
TGTATAGCATCAAATAAGTC+AGG 0.495328 5.1:-70251164 None:intergenic
TAATTTCATGAGGCATAGAA+TGG 0.500689 5.1:+70251244 MS.gene016921:CDS
ACAAATCCCTCATCACAGTA+AGG 0.515163 5.1:-70251065 None:intergenic
CCATTAGGCAAGGTTAGTCC+AGG 0.516692 5.1:-70251380 None:intergenic
CCAGTTCCTGAACAGGGCTG+GGG 0.517490 5.1:+70251314 MS.gene016921:CDS
CAATTGAAGATCGTGCAGCT+GGG 0.527634 5.1:-70251511 None:intergenic
CTCTCCCGAGCGTGATGGAA+AGG 0.532737 5.1:-70251129 None:intergenic
TCACCGAAATCGCCACGCCT+AGG 0.535999 5.1:-70251419 None:intergenic
AAATACCTTTCCATCACGCT+CGG 0.536583 5.1:+70251124 MS.gene016921:CDS
GCAGACTGGACAATGTGAAT+CGG 0.544019 5.1:-70251576 None:intergenic
GTTTGCCCTTACTGTGATGA+GGG 0.546875 5.1:+70251059 MS.gene016921:CDS
GTGGTGGAAATGGTAGCTCA+AGG 0.554250 5.1:+70251768 MS.gene016921:CDS
AGTCGGCCAATTGTGCTTGA+AGG 0.556833 5.1:+70251026 MS.gene016921:CDS
AATAATCAATATTCTGAGAG+TGG 0.558489 5.1:+70251872 MS.gene016921:CDS
GTTGAAGAAATAATGTCAAG+TGG 0.558708 5.1:+70250978 None:intergenic
ATGGAACACGTTATAATGGA+TGG 0.563370 5.1:+70251930 MS.gene016921:CDS
ACCCAAACTTTGATGTTAGA+GGG 0.565011 5.1:+70251276 MS.gene016921:CDS
GTCGGCCAATTGTGCTTGAA+GGG 0.565165 5.1:+70251027 MS.gene016921:CDS
AACAACTAACCATGAATGAT+CGG 0.565917 5.1:+70251477 MS.gene016921:CDS
AACGGCCACAGAAATGAAAA+CGG 0.567397 5.1:-70251830 None:intergenic
AAATGGTAGCTCAAGGGGCA+GGG 0.567954 5.1:+70251775 MS.gene016921:CDS
TGGTGGAAATGGTAGCTCAA+GGG 0.569039 5.1:+70251769 MS.gene016921:CDS
GCCTGCGTGATCCTAATAGT+AGG 0.572303 5.1:-70251542 None:intergenic
AAGGAACAATACATTCAGAA+TGG 0.573178 5.1:-70251655 None:intergenic
AATTGAAGATCGTGCAGCTG+GGG 0.576072 5.1:-70251510 None:intergenic
AATACCTTTCCATCACGCTC+GGG 0.577219 5.1:+70251125 MS.gene016921:CDS
GGTGGAAATGGTAGCTCAAG+GGG 0.581164 5.1:+70251770 MS.gene016921:CDS
CGTTTGCCCTTACTGTGATG+AGG 0.582429 5.1:+70251058 MS.gene016921:CDS
TTAAACTCTCCCGAGCGTGA+TGG 0.588811 5.1:-70251134 None:intergenic
TATTGTTTGAACTAGAAGAA+CGG 0.600491 5.1:-70251853 None:intergenic
TCTCGGGCTTCCATTAGGCA+AGG 0.606325 5.1:-70251390 None:intergenic
CCGAGAGGTGGTCCTAGGCG+TGG 0.606422 5.1:+70251407 MS.gene016921:CDS
ATTGAAGATCGTGCAGCTGG+GGG 0.607854 5.1:-70251509 None:intergenic
GAGAGTGACCGTCCTAACAA+TGG 0.610294 5.1:+70251797 MS.gene016921:CDS
TCTGTCCCAGTTCCTGAACA+GGG 0.612021 5.1:+70251308 MS.gene016921:CDS
CAAATCCCTCATCACAGTAA+GGG 0.613393 5.1:-70251064 None:intergenic
CGAGAACTCATCTGTCCTTG+TGG 0.617859 5.1:-70251725 None:intergenic
GAATGGTAAATATGGTCACA+TGG 0.629134 5.1:-70251638 None:intergenic
AGTAGTCGAAGCAACAGTGG+TGG 0.638220 5.1:+70251752 MS.gene016921:CDS
TGTCAAGTGGAGCAACACAT+TGG 0.641943 5.1:+70250991 MS.gene016921:CDS
CCTAATGGAAGCCCGAGAGG+TGG 0.642945 5.1:+70251395 MS.gene016921:CDS
GTGACCGTCCTAACAATGGA+AGG 0.643870 5.1:+70251801 MS.gene016921:CDS
ATTGGTGTTATGAATGCAGT+CGG 0.644987 5.1:+70251009 MS.gene016921:CDS
CTTGTGGTGGTAGCTCAACA+CGG 0.645208 5.1:-70251709 None:intergenic
CGTGTTGAGCTACCACCACA+AGG 0.646648 5.1:+70251710 MS.gene016921:CDS
TTGCCTAATGGAAGCCCGAG+AGG 0.670811 5.1:+70251392 MS.gene016921:CDS
GAACTCATCTGTCCTTGTGG+TGG 0.671137 5.1:-70251722 None:intergenic
AATCAATATTCTGAGAGTGG+AGG 0.671363 5.1:+70251875 MS.gene016921:CDS
CAAGGACAGATGAGTTCTCG+TGG 0.679090 5.1:+70251728 MS.gene016921:CDS
GAGTTATGTTGAACCAACCA+AGG 0.692810 5.1:-70251674 None:intergenic
GCCTACTATTAGGATCACGC+AGG 0.693456 5.1:+70251541 MS.gene016921:CDS
CAAGAAATTAACGAGATGCG+AGG 0.695840 5.1:+70251089 MS.gene016921:CDS
CAAACTTTGATGTTAGAGGG+AGG 0.698975 5.1:+70251279 MS.gene016921:CDS
ATGCTGTTGATAATTTCATG+AGG 0.699793 5.1:+70251234 MS.gene016921:CDS
GGTAGTAGTCGAAGCAACAG+TGG 0.708997 5.1:+70251749 MS.gene016921:CDS
TTTGAACTAGAAGAACGGAA+CGG 0.720066 5.1:-70251848 None:intergenic
CTAACCATGAATGATCGGCG+TGG 0.777204 5.1:+70251482 MS.gene016921:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TACATTCAGAATGGTAAATA+TGG - chr5.1:70251649-70251668 None:intergenic 25.0%
! TATTGTTTGAACTAGAAGAA+CGG - chr5.1:70251856-70251875 None:intergenic 25.0%
! TGACCATGAAATACTAAAAA+AGG - chr5.1:70251356-70251375 None:intergenic 25.0%
! TGCAAAGAAAAATTTGAACT+TGG + chr5.1:70251596-70251615 MS.gene016921:CDS 25.0%
!! AATAATCAATATTCTGAGAG+TGG + chr5.1:70251872-70251891 MS.gene016921:CDS 25.0%
!! AATAATGGAACACGTTATAA+TGG + chr5.1:70251926-70251945 MS.gene016921:CDS 25.0%
!!! GATTTCGGTGATTATTTTAT+GGG + chr5.1:70251431-70251450 MS.gene016921:CDS 25.0%
AACAACTAACCATGAATGAT+CGG + chr5.1:70251477-70251496 MS.gene016921:CDS 30.0%
AAGGAACAATACATTCAGAA+TGG - chr5.1:70251658-70251677 None:intergenic 30.0%
ATTCTGAATGTATTGTTCCT+TGG + chr5.1:70251657-70251676 MS.gene016921:CDS 30.0%
TAATTTCATGAGGCATAGAA+TGG + chr5.1:70251244-70251263 MS.gene016921:CDS 30.0%
TATAACGTGTTCCATTATTC+TGG - chr5.1:70251925-70251944 None:intergenic 30.0%
TGTATAGCATCAAATAAGTC+AGG - chr5.1:70251167-70251186 None:intergenic 30.0%
! TCAAATTTTTCTTTGCAGAC+TGG - chr5.1:70251593-70251612 None:intergenic 30.0%
!! ATGCTGTTGATAATTTCATG+AGG + chr5.1:70251234-70251253 MS.gene016921:CDS 30.0%
!! CGATTTCGGTGATTATTTTA+TGG + chr5.1:70251430-70251449 MS.gene016921:CDS 30.0%
!!! GGTCCTTTTTTAGTATTTCA+TGG + chr5.1:70251350-70251369 MS.gene016921:CDS 30.0%
ACCCAAACTTTGATGTTAGA+GGG + chr5.1:70251276-70251295 MS.gene016921:CDS 35.0%
GAATGGTAAATATGGTCACA+TGG - chr5.1:70251641-70251660 None:intergenic 35.0%
TCCCTCTAACATCAAAGTTT+GGG - chr5.1:70251280-70251299 None:intergenic 35.0%
TGAATGTATTGTTCCTTGGT+TGG + chr5.1:70251661-70251680 MS.gene016921:CDS 35.0%
TTGATGCAATGCCTACTATT+AGG + chr5.1:70251531-70251550 MS.gene016921:CDS 35.0%
TTTGAACTAGAAGAACGGAA+CGG - chr5.1:70251851-70251870 None:intergenic 35.0%
! AATCAATATTCTGAGAGTGG+AGG + chr5.1:70251875-70251894 MS.gene016921:CDS 35.0%
! ATGCTATACATGCTTTTGTG+GGG + chr5.1:70251177-70251196 MS.gene016921:CDS 35.0%
! ATGGAACACGTTATAATGGA+TGG + chr5.1:70251930-70251949 MS.gene016921:CDS 35.0%
! GAAATCCGTTTTCATTTCTG+TGG + chr5.1:70251825-70251844 MS.gene016921:CDS 35.0%
! GATGCTATACATGCTTTTGT+GGG + chr5.1:70251176-70251195 MS.gene016921:CDS 35.0%
! TGATGCTATACATGCTTTTG+TGG + chr5.1:70251175-70251194 MS.gene016921:CDS 35.0%
!! ATTGGTGTTATGAATGCAGT+CGG + chr5.1:70251009-70251028 MS.gene016921:CDS 35.0%
!! GGTGATTATTTTATGGGTTC+TGG + chr5.1:70251437-70251456 MS.gene016921:CDS 35.0%
!!! TTATTTTATGGGTTCTGGAC+TGG + chr5.1:70251442-70251461 MS.gene016921:CDS 35.0%
AAATACCTTTCCATCACGCT+CGG + chr5.1:70251124-70251143 MS.gene016921:CDS 40.0%
AACGGCCACAGAAATGAAAA+CGG - chr5.1:70251833-70251852 None:intergenic 40.0%
ACAAATCCCTCATCACAGTA+AGG - chr5.1:70251068-70251087 None:intergenic 40.0%
ACCAATGCTGACCAGAATAA+TGG + chr5.1:70251911-70251930 MS.gene016921:CDS 40.0%
CAAACTTTGATGTTAGAGGG+AGG + chr5.1:70251279-70251298 MS.gene016921:CDS 40.0%
CAAATCCCTCATCACAGTAA+GGG - chr5.1:70251067-70251086 None:intergenic 40.0%
CAAGAAATTAACGAGATGCG+AGG + chr5.1:70251089-70251108 MS.gene016921:CDS 40.0%
CACCCAAACTTTGATGTTAG+AGG + chr5.1:70251275-70251294 MS.gene016921:CDS 40.0%
CTCCCTCTAACATCAAAGTT+TGG - chr5.1:70251281-70251300 None:intergenic 40.0%
GAGTTATGTTGAACCAACCA+AGG - chr5.1:70251677-70251696 None:intergenic 40.0%
GGATTTCTCCTTCCATTGTT+AGG - chr5.1:70251812-70251831 None:intergenic 40.0%
GTATTTCATGGTCAAGTTCC+TGG + chr5.1:70251362-70251381 MS.gene016921:CDS 40.0%
TCCATTATTCTGGTCAGCAT+TGG - chr5.1:70251915-70251934 None:intergenic 40.0%
TGACAATAGGTTTGGGCTTA+TGG + chr5.1:70251211-70251230 MS.gene016921:CDS 40.0%
TTCTCCTTCCATTGTTAGGA+CGG - chr5.1:70251808-70251827 None:intergenic 40.0%
! AGAGGTTCTGACAATAGGTT+TGG + chr5.1:70251203-70251222 MS.gene016921:CDS 40.0%
! GAGGTTCTGACAATAGGTTT+GGG + chr5.1:70251204-70251223 MS.gene016921:CDS 40.0%
!!! CGTGATGGAAAGGTATTTTG+AGG - chr5.1:70251122-70251141 None:intergenic 40.0%
AATACCTTTCCATCACGCTC+GGG + chr5.1:70251125-70251144 MS.gene016921:CDS 45.0%
AATTGAAGATCGTGCAGCTG+GGG - chr5.1:70251513-70251532 None:intergenic 45.0%
CAATTGAAGATCGTGCAGCT+GGG - chr5.1:70251514-70251533 None:intergenic 45.0%
GCAGACTGGACAATGTGAAT+CGG - chr5.1:70251579-70251598 None:intergenic 45.0%
GTTTGCCCTTACTGTGATGA+GGG + chr5.1:70251059-70251078 MS.gene016921:CDS 45.0%
TCAATTGAAGATCGTGCAGC+TGG - chr5.1:70251515-70251534 None:intergenic 45.0%
TCTCTCCCTTCAAGCACAAT+TGG - chr5.1:70251035-70251054 None:intergenic 45.0%
TGGTGGAAATGGTAGCTCAA+GGG + chr5.1:70251769-70251788 MS.gene016921:CDS 45.0%
TGTCAAGTGGAGCAACACAT+TGG + chr5.1:70250991-70251010 MS.gene016921:CDS 45.0%
TTCATGAGGCATAGAATGGC+TGG + chr5.1:70251248-70251267 MS.gene016921:CDS 45.0%
AAATGGTAGCTCAAGGGGCA+GGG + chr5.1:70251775-70251794 MS.gene016921:CDS 50.0%
AGTAGTCGAAGCAACAGTGG+TGG + chr5.1:70251752-70251771 MS.gene016921:CDS 50.0%
AGTCGGCCAATTGTGCTTGA+AGG + chr5.1:70251026-70251045 MS.gene016921:CDS 50.0%
ATGGTCACATGGCATCTCTC+TGG - chr5.1:70251630-70251649 None:intergenic 50.0%
ATTGAAGATCGTGCAGCTGG+GGG - chr5.1:70251512-70251531 None:intergenic 50.0%
CAAGGACAGATGAGTTCTCG+TGG + chr5.1:70251728-70251747 MS.gene016921:CDS 50.0%
CCTGGACTAACCTTGCCTAA+TGG + chr5.1:70251380-70251399 MS.gene016921:CDS 50.0%
CGAGAACTCATCTGTCCTTG+TGG - chr5.1:70251728-70251747 None:intergenic 50.0%
CGTTTGCCCTTACTGTGATG+AGG + chr5.1:70251058-70251077 MS.gene016921:CDS 50.0%
CTAACCATGAATGATCGGCG+TGG + chr5.1:70251482-70251501 MS.gene016921:CDS 50.0%
CTTGTGGTGGTAGCTCAACA+CGG - chr5.1:70251712-70251731 None:intergenic 50.0%
GAACTCATCTGTCCTTGTGG+TGG - chr5.1:70251725-70251744 None:intergenic 50.0%
GAGAGTGACCGTCCTAACAA+TGG + chr5.1:70251797-70251816 MS.gene016921:CDS 50.0%
GCCTACTATTAGGATCACGC+AGG + chr5.1:70251541-70251560 MS.gene016921:CDS 50.0%
GCCTGCGTGATCCTAATAGT+AGG - chr5.1:70251545-70251564 None:intergenic 50.0%
GGTAGTAGTCGAAGCAACAG+TGG + chr5.1:70251749-70251768 MS.gene016921:CDS 50.0%
GGTGGAAATGGTAGCTCAAG+GGG + chr5.1:70251770-70251789 MS.gene016921:CDS 50.0%
GTCGGCCAATTGTGCTTGAA+GGG + chr5.1:70251027-70251046 MS.gene016921:CDS 50.0%
GTGACCGTCCTAACAATGGA+AGG + chr5.1:70251801-70251820 MS.gene016921:CDS 50.0%
TCTGTCCCAGTTCCTGAACA+GGG + chr5.1:70251308-70251327 MS.gene016921:CDS 50.0%
TTAAACTCTCCCGAGCGTGA+TGG - chr5.1:70251137-70251156 None:intergenic 50.0%
TTCTGTCCCAGTTCCTGAAC+AGG + chr5.1:70251307-70251326 MS.gene016921:CDS 50.0%
! CCATTAGGCAAGGTTAGTCC+AGG - chr5.1:70251383-70251402 None:intergenic 50.0%
! CGAAGCAACAGTGGTGGAAA+TGG + chr5.1:70251758-70251777 MS.gene016921:CDS 50.0%
! GGCACAGAGGTTCTGACAAT+AGG + chr5.1:70251198-70251217 MS.gene016921:CDS 50.0%
! GTGGTGGAAATGGTAGCTCA+AGG + chr5.1:70251768-70251787 MS.gene016921:CDS 50.0%
CGTGTTGAGCTACCACCACA+AGG + chr5.1:70251710-70251729 MS.gene016921:CDS 55.0%
GAAATGGTAGCTCAAGGGGC+AGG + chr5.1:70251774-70251793 MS.gene016921:CDS 55.0%
GGTAGCTCAACACGGCAAAC+AGG - chr5.1:70251704-70251723 None:intergenic 55.0%
TCTCGGGCTTCCATTAGGCA+AGG - chr5.1:70251393-70251412 None:intergenic 55.0%
TGGACCACGCCGATCATTCA+TGG - chr5.1:70251489-70251508 None:intergenic 55.0%
TTGCCTAATGGAAGCCCGAG+AGG + chr5.1:70251392-70251411 MS.gene016921:CDS 55.0%
! CATGCTTTTGTGGGGCACAG+AGG + chr5.1:70251185-70251204 MS.gene016921:CDS 55.0%
! GGCTGGGGCGTTTATAGTTC+TGG + chr5.1:70251329-70251348 MS.gene016921:CDS 55.0%
CCACCTCTCGGGCTTCCATT+AGG - chr5.1:70251398-70251417 None:intergenic 60.0%
CCAGTTCCTGAACAGGGCTG+GGG + chr5.1:70251314-70251333 MS.gene016921:CDS 60.0%
CCCAGCCCTGTTCAGGAACT+GGG - chr5.1:70251316-70251335 None:intergenic 60.0%
CCCAGTTCCTGAACAGGGCT+GGG + chr5.1:70251313-70251332 MS.gene016921:CDS 60.0%
CCTAATGGAAGCCCGAGAGG+TGG + chr5.1:70251395-70251414 MS.gene016921:CDS 60.0%
GGTCCTAGGCGTGGCGATTT+CGG + chr5.1:70251416-70251435 MS.gene016921:CDS 60.0%
TAAACGCCCCAGCCCTGTTC+AGG - chr5.1:70251323-70251342 None:intergenic 60.0%
TCACCGAAATCGCCACGCCT+AGG - chr5.1:70251422-70251441 None:intergenic 60.0%
TCCCAGTTCCTGAACAGGGC+TGG + chr5.1:70251312-70251331 MS.gene016921:CDS 60.0%
! CTCTCCCGAGCGTGATGGAA+AGG - chr5.1:70251132-70251151 None:intergenic 60.0%
CACGCCTAGGACCACCTCTC+GGG - chr5.1:70251409-70251428 None:intergenic 65.0%
CCACGCCTAGGACCACCTCT+CGG - chr5.1:70251410-70251429 None:intergenic 65.0%
CCCCAGCCCTGTTCAGGAAC+TGG - chr5.1:70251317-70251336 None:intergenic 65.0%
GAAGATCGTGCAGCTGGGGG+TGG - chr5.1:70251509-70251528 None:intergenic 65.0%
GAAGCCCGAGAGGTGGTCCT+AGG + chr5.1:70251402-70251421 MS.gene016921:CDS 65.0%
CCGAGAGGTGGTCCTAGGCG+TGG + chr5.1:70251407-70251426 MS.gene016921:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 70250990 70251967 70250990 ID=MS.gene016921
chr5.1 mRNA 70250990 70251967 70250990 ID=MS.gene016921.t1;Parent=MS.gene016921
chr5.1 exon 70250990 70251967 70250990 ID=MS.gene016921.t1.exon1;Parent=MS.gene016921.t1
chr5.1 CDS 70250990 70251967 70250990 ID=cds.MS.gene016921.t1;Parent=MS.gene016921.t1
Gene Sequence

>MS.gene016921

ATGTCAAGTGGAGCAACACATTGGTGTTATGAATGCAGTCGGCCAATTGTGCTTGAAGGGAGAGACATCGTTTGCCCTTACTGTGATGAGGGATTTGTACAAGAAATTAACGAGATGCGAGGCACTGCACCTCAAAATACCTTTCCATCACGCTCGGGAGAGTTTAATCAAATGCCTGACTTATTTGATGCTATACATGCTTTTGTGGGGCACAGAGGTTCTGACAATAGGTTTGGGCTTATGGATGCTGTTGATAATTTCATGAGGCATAGAATGGCTGGAATGCACCCAAACTTTGATGTTAGAGGGAGGTCTAGTTCTGTCCCAGTTCCTGAACAGGGCTGGGGCGTTTATAGTTCTGGTCCTTTTTTAGTATTTCATGGTCAAGTTCCTGGACTAACCTTGCCTAATGGAAGCCCGAGAGGTGGTCCTAGGCGTGGCGATTTCGGTGATTATTTTATGGGTTCTGGACTGGAAGAACTTATTGAACAACTAACCATGAATGATCGGCGTGGTCCACCCCCAGCTGCACGATCTTCAATTGATGCAATGCCTACTATTAGGATCACGCAGGCGCATCTTCGCTCCGATTCACATTGTCCAGTCTGCAAAGAAAAATTTGAACTTGGTTCTGAAGCCAGAGAGATGCCATGTGACCATATTTACCATTCTGAATGTATTGTTCCTTGGTTGGTTCAACATAACTCGTGTCCTGTTTGCCGTGTTGAGCTACCACCACAAGGACAGATGAGTTCTCGTGGTAGTAGTCGAAGCAACAGTGGTGGAAATGGTAGCTCAAGGGGCAGGGAGAGTGACCGTCCTAACAATGGAAGGAGAAATCCGTTTTCATTTCTGTGGCCGTTCCGTTCTTCTAGTTCAAACAATAATCAATATTCTGAGAGTGGAGGTAGCAGCTCTTCAACCAATGCTGACCAGAATAATGGAACACGTTATAATGGATGGCATTATGATAACTAA
Protein sequence

>MS.gene016921.t1

MSSGATHWCYECSRPIVLEGRDIVCPYCDEGFVQEINEMRGTAPQNTFPSRSGEFNQMPDLFDAIHAFVGHRGSDNRFGLMDAVDNFMRHRMAGMHPNFDVRGRSSSVPVPEQGWGVYSSGPFLVFHGQVPGLTLPNGSPRGGPRRGDFGDYFMGSGLEELIEQLTMNDRRGPPPAARSSIDAMPTIRITQAHLRSDSHCPVCKEKFELGSEAREMPCDHIYHSECIVPWLVQHNSCPVCRVELPPQGQMSSRGSSRSNSGGNGSSRGRESDRPNNGRRNPFSFLWPFRSSSSNNNQYSESGGSSSSTNADQNNGTRYNGWHYDN