Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017955.t1 | XP_003625216.1 | 100 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.40E-61 | 245.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017955.t1 | Q9STY6 | 89.3 | 122 | 13 | 0 | 1 | 122 | 1 | 122 | 2.2e-55 | 216.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017955.t1 | A2Q4V6 | 100.0 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.0e-61 | 245.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene068750 | MS.gene017955 | PPI |
| MS.gene045681 | MS.gene017955 | PPI |
| MS.gene017955 | MS.gene031439 | PPI |
| MS.gene017955 | MS.gene04591 | PPI |
| MS.gene017955 | MS.gene072375 | PPI |
| MS.gene72479 | MS.gene017955 | PPI |
| MS.gene017955 | MS.gene059832 | PPI |
| MS.gene017955 | MS.gene77739 | PPI |
| MS.gene017955 | MS.gene78060 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017955.t1 | MTR_7g092720 | 100.000 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.11e-87 | 250 |
| MS.gene017955.t1 | MTR_0157s0070 | 98.361 | 122 | 2 | 0 | 1 | 122 | 1 | 122 | 7.09e-87 | 248 |
| MS.gene017955.t1 | MTR_4g034030 | 98.361 | 122 | 2 | 0 | 1 | 122 | 1 | 122 | 7.09e-87 | 248 |
| MS.gene017955.t1 | MTR_2g085090 | 97.500 | 120 | 3 | 0 | 3 | 122 | 4 | 123 | 9.83e-84 | 240 |
| MS.gene017955.t1 | MTR_1g091057 | 97.500 | 120 | 3 | 0 | 3 | 122 | 4 | 123 | 9.83e-84 | 240 |
| MS.gene017955.t1 | MTR_4g034030 | 98.824 | 85 | 1 | 0 | 38 | 122 | 3 | 87 | 3.02e-58 | 174 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017955.t1 | AT3G47370 | 89.344 | 122 | 13 | 0 | 1 | 122 | 1 | 122 | 2.23e-76 | 222 |
| MS.gene017955.t1 | AT3G47370 | 89.344 | 122 | 13 | 0 | 1 | 122 | 1 | 122 | 2.23e-76 | 222 |
| MS.gene017955.t1 | AT3G47370 | 89.344 | 122 | 13 | 0 | 1 | 122 | 1 | 122 | 2.23e-76 | 222 |
| MS.gene017955.t1 | AT5G62300 | 89.256 | 121 | 13 | 0 | 2 | 122 | 4 | 124 | 1.92e-75 | 219 |
| MS.gene017955.t1 | AT5G62300 | 89.256 | 121 | 13 | 0 | 2 | 122 | 4 | 124 | 1.92e-75 | 219 |
| MS.gene017955.t1 | AT3G45030 | 89.256 | 121 | 13 | 0 | 2 | 122 | 4 | 124 | 1.92e-75 | 219 |
Find 42 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATTAGTTTGTGCTGATT+TGG | 0.351954 | 7.1:-14732347 | MS.gene017955:intron |
| AAAGGGTGATCCTAATCTTA+TGG | 0.356442 | 7.1:+14732480 | None:intergenic |
| TTCTCCACAAGGAGTCTTCC+TGG | 0.359877 | 7.1:+14732249 | None:intergenic |
| GTAATCTGTTTAACAACATC+TGG | 0.381438 | 7.1:+14731230 | None:intergenic |
| AATCTTATGGATCTGCTCTT+GGG | 0.403761 | 7.1:+14732493 | None:intergenic |
| TAATCTTATGGATCTGCTCT+TGG | 0.405883 | 7.1:+14732492 | None:intergenic |
| GGATTCCTCCAAACCAGGCT+TGG | 0.415376 | 7.1:+14732514 | None:intergenic |
| GAAACCCACCAAGCCTGGTT+TGG | 0.420702 | 7.1:-14732522 | MS.gene017955:CDS |
| TCCTCCAAACCAGGCTTGGT+GGG | 0.429749 | 7.1:+14732518 | None:intergenic |
| TTCCTCCAAACCAGGCTTGG+TGG | 0.433644 | 7.1:+14732517 | None:intergenic |
| GTGATATGAAGAACCTTAGT+AGG | 0.436083 | 7.1:+14732274 | None:intergenic |
| TTAGAGTGAAGGGTCCTGTT+AGG | 0.436691 | 7.1:-14732300 | MS.gene017955:CDS |
| ACCTTAGTAGGCATCCTAAC+AGG | 0.444549 | 7.1:+14732286 | None:intergenic |
| AAGAGCAGATCCATAAGATT+AGG | 0.460004 | 7.1:-14732490 | MS.gene017955:CDS |
| TTGTTACAGGTACCAACACC+TGG | 0.473935 | 7.1:-14731304 | MS.gene017955:intron |
| TTTGACATGCTTGGAGGAAA+GGG | 0.476236 | 7.1:+14732463 | None:intergenic |
| ACCAGGTTCGATGGTGATAG+AGG | 0.489959 | 7.1:+14731208 | None:intergenic |
| GTTTGTGCTGATTTGGTTCG+TGG | 0.496204 | 7.1:-14732340 | MS.gene017955:CDS |
| GCGATGAAACCCACCAAGCC+TGG | 0.496657 | 7.1:-14732527 | MS.gene017955:CDS |
| CGAACCTGGTGTCGAGGTTG+AGG | 0.501443 | 7.1:-14731195 | MS.gene017955:CDS |
| AGGAAGACTCCTTGTGGAGA+AGG | 0.508893 | 7.1:-14732247 | MS.gene017955:intron |
| TCCTGTTAGGATGCCTACTA+AGG | 0.508963 | 7.1:-14732287 | MS.gene017955:CDS |
| ACCCACCAAGCCTGGTTTGG+AGG | 0.512859 | 7.1:-14732519 | MS.gene017955:CDS |
| TCTTGGGATTCCTCCAAACC+AGG | 0.514536 | 7.1:+14732509 | None:intergenic |
| ACCTCTATCACCATCGAACC+TGG | 0.519945 | 7.1:-14731209 | MS.gene017955:CDS |
| TAATCTGTTTAACAACATCT+GGG | 0.529679 | 7.1:+14731231 | None:intergenic |
| AACCTCGACACCAGGTTCGA+TGG | 0.552766 | 7.1:+14731199 | None:intergenic |
| CACCATCGAACCTGGTGTCG+AGG | 0.559437 | 7.1:-14731201 | MS.gene017955:CDS |
| TGATTTGGTTCGTGGTGCTA+AGG | 0.559992 | 7.1:-14732332 | MS.gene017955:CDS |
| CGATAACTCTCTTGTGCACA+CGG | 0.563868 | 7.1:+14731267 | None:intergenic |
| GGATAAGCATCTTAGAGTGA+AGG | 0.564807 | 7.1:-14732311 | MS.gene017955:CDS |
| TTCAAATCTATCCCAGGTGT+TGG | 0.568966 | 7.1:+14731292 | None:intergenic |
| GATAAGCATCTTAGAGTGAA+GGG | 0.578400 | 7.1:-14732310 | MS.gene017955:CDS |
| GTAACCTCAACCTCGACACC+AGG | 0.583580 | 7.1:+14731191 | None:intergenic |
| CAACATCTGGGGAACTGTAG+AGG | 0.590461 | 7.1:+14731243 | None:intergenic |
| ACGGAGTTCAAATCTATCCC+AGG | 0.607565 | 7.1:+14731286 | None:intergenic |
| AGGTTCTTCATATCACTACC+AGG | 0.631821 | 7.1:-14732267 | MS.gene017955:CDS |
| TTAAGATCAAGCATCTGCAA+TGG | 0.639875 | 7.1:+14731169 | None:intergenic |
| AATGAAATACCTTCTCCACA+AGG | 0.646547 | 7.1:+14732238 | None:intergenic |
| ACTACCAGGAAGACTCCTTG+TGG | 0.667890 | 7.1:-14732253 | MS.gene017955:CDS |
| TGTTACAGGTACCAACACCT+GGG | 0.689167 | 7.1:-14731303 | MS.gene017955:intron |
| AATCTGTTTAACAACATCTG+GGG | 0.693040 | 7.1:+14731232 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTATAATTATTTGATGAA+AGG | - | chr7.1:14731542-14731561 | MS.gene017955:intron | 10.0% |
| !!! | GTTCTCTTTAAAATAATAAA+TGG | - | chr7.1:14731845-14731864 | MS.gene017955:intron | 15.0% |
| !! | ATAATAACAAAATGCAAATG+AGG | + | chr7.1:14732220-14732239 | None:intergenic | 20.0% |
| !!! | TTTAATTCAGTTTTGTTGAA+TGG | - | chr7.1:14732047-14732066 | MS.gene017955:intron | 20.0% |
| ! | AATTATCCTACAACCTTAAT+TGG | - | chr7.1:14731949-14731968 | MS.gene017955:intron | 25.0% |
| ! | TAATCTGTTTAACAACATCT+GGG | + | chr7.1:14732486-14732505 | None:intergenic | 25.0% |
| !! | TGAGAGCATAAAAAACATAA+AGG | + | chr7.1:14731309-14731328 | None:intergenic | 25.0% |
| !!! | GCATTTTGTTATTATGCTAT+TGG | - | chr7.1:14732225-14732244 | MS.gene017955:intron | 25.0% |
| !!! | TGTTTCTGAACAAATTTTGA+AGG | - | chr7.1:14732092-14732111 | MS.gene017955:intron | 25.0% |
| AATCTGTTTAACAACATCTG+GGG | + | chr7.1:14732485-14732504 | None:intergenic | 30.0% | |
| AGATTAGAAACATCATTGAG+AGG | - | chr7.1:14731580-14731599 | MS.gene017955:intron | 30.0% | |
| ATTTGATGAAAGGTAGTCAA+TGG | - | chr7.1:14731552-14731571 | MS.gene017955:intron | 30.0% | |
| GATTAGAAACATCATTGAGA+GGG | - | chr7.1:14731581-14731600 | MS.gene017955:intron | 30.0% | |
| GTAATCTGTTTAACAACATC+TGG | + | chr7.1:14732487-14732506 | None:intergenic | 30.0% | |
| TAAAGAGAACAAACCAAACA+CGG | + | chr7.1:14731835-14731854 | None:intergenic | 30.0% | |
| TCCTAATATGGCTTACAAAT+GGG | - | chr7.1:14731722-14731741 | MS.gene017955:intron | 30.0% | |
| ! | GTTTTTCTAAGCTCCTAATA+TGG | - | chr7.1:14731710-14731729 | MS.gene017955:intron | 30.0% |
| !! | GTTATTAGTTTGTGCTGATT+TGG | - | chr7.1:14731367-14731386 | MS.gene017955:intron | 30.0% |
| AAACCAATAGACACAGAAGA+AGG | + | chr7.1:14731886-14731905 | None:intergenic | 35.0% | |
| AAAGGGTGATCCTAATCTTA+TGG | + | chr7.1:14731237-14731256 | None:intergenic | 35.0% | |
| AAATACGATATAGCCGTGTT+TGG | - | chr7.1:14731819-14731838 | MS.gene017955:intron | 35.0% | |
| AACCAATAGACACAGAAGAA+GGG | + | chr7.1:14731885-14731904 | None:intergenic | 35.0% | |
| AAGAGCAGATCCATAAGATT+AGG | - | chr7.1:14731224-14731243 | MS.gene017955:CDS | 35.0% | |
| AATCTTATGGATCTGCTCTT+GGG | + | chr7.1:14731224-14731243 | None:intergenic | 35.0% | |
| AATGAAATACCTTCTCCACA+AGG | + | chr7.1:14731479-14731498 | None:intergenic | 35.0% | |
| ACCCATTTGTAAGCCATATT+AGG | + | chr7.1:14731726-14731745 | None:intergenic | 35.0% | |
| ATCCTACACTTCAATGACAA+TGG | + | chr7.1:14732332-14732351 | None:intergenic | 35.0% | |
| ATGACCTTACTTCTTGTTAC+AGG | - | chr7.1:14732397-14732416 | MS.gene017955:intron | 35.0% | |
| CATGTCAAAAATCTCGAGAA+GGG | - | chr7.1:14731265-14731284 | MS.gene017955:CDS | 35.0% | |
| CTCCTAATATGGCTTACAAA+TGG | - | chr7.1:14731721-14731740 | MS.gene017955:intron | 35.0% | |
| GATAAGCATCTTAGAGTGAA+GGG | - | chr7.1:14731404-14731423 | MS.gene017955:intron | 35.0% | |
| GCATCTAAATTTCCATAAGC+AGG | + | chr7.1:14731775-14731794 | None:intergenic | 35.0% | |
| GTGATATGAAGAACCTTAGT+AGG | + | chr7.1:14731443-14731462 | None:intergenic | 35.0% | |
| TAATCTTATGGATCTGCTCT+TGG | + | chr7.1:14731225-14731244 | None:intergenic | 35.0% | |
| !! | AATTTTGAAGGACTCGACTA+CGG | - | chr7.1:14732104-14732123 | MS.gene017955:intron | 35.0% |
| !!! | CTTGATAGTGCTATGTTTTC+TGG | - | chr7.1:14732289-14732308 | MS.gene017955:CDS | 35.0% |
| ACCAATAGACACAGAAGAAG+GGG | + | chr7.1:14731884-14731903 | None:intergenic | 40.0% | |
| AGCCATTGTCATTGAAGTGT+AGG | - | chr7.1:14732327-14732346 | MS.gene017955:CDS | 40.0% | |
| AGGTTCTTCATATCACTACC+AGG | - | chr7.1:14731447-14731466 | MS.gene017955:intron | 40.0% | |
| CACTATCAAGTATTCAGCAG+AGG | + | chr7.1:14732279-14732298 | None:intergenic | 40.0% | |
| CGTATGCCAATTAAGGTTGT+AGG | + | chr7.1:14731958-14731977 | None:intergenic | 40.0% | |
| GAGAGGGAACTGTTGAAAAT+AGG | - | chr7.1:14731597-14731616 | MS.gene017955:intron | 40.0% | |
| GCATGTCAAAAATCTCGAGA+AGG | - | chr7.1:14731264-14731283 | MS.gene017955:CDS | 40.0% | |
| GGATAAGCATCTTAGAGTGA+AGG | - | chr7.1:14731403-14731422 | MS.gene017955:intron | 40.0% | |
| GGTACCTGTAACAAGAAGTA+AGG | + | chr7.1:14732404-14732423 | None:intergenic | 40.0% | |
| TACGTGTGAGAGTAAAAGGA+AGG | - | chr7.1:14731974-14731993 | MS.gene017955:intron | 40.0% | |
| TCAAGTATTCAGCAGAGGTT+TGG | + | chr7.1:14732274-14732293 | None:intergenic | 40.0% | |
| TCTCACACGTATGCCAATTA+AGG | + | chr7.1:14731965-14731984 | None:intergenic | 40.0% | |
| TGCGCATAGAAGAGAATCAA+CGG | + | chr7.1:14732183-14732202 | None:intergenic | 40.0% | |
| TTCAAATCTATCCCAGGTGT+TGG | + | chr7.1:14732425-14732444 | None:intergenic | 40.0% | |
| TTTGACATGCTTGGAGGAAA+GGG | + | chr7.1:14731254-14731273 | None:intergenic | 40.0% | |
| ! | CTCGAGATTTTTGACATGCT+TGG | + | chr7.1:14731263-14731282 | None:intergenic | 40.0% |
| ! | GAGATTTTTGACATGCTTGG+AGG | + | chr7.1:14731260-14731279 | None:intergenic | 40.0% |
| ! | TTTTGACATGCTTGGAGGAA+AGG | + | chr7.1:14731255-14731274 | None:intergenic | 40.0% |
| !! | ATTTTGAAGGACTCGACTAC+GGG | - | chr7.1:14732105-14732124 | MS.gene017955:intron | 40.0% |
| ACCTTAGTAGGCATCCTAAC+AGG | + | chr7.1:14731431-14731450 | None:intergenic | 45.0% | |
| ACGGAGTTCAAATCTATCCC+AGG | + | chr7.1:14732431-14732450 | None:intergenic | 45.0% | |
| CGATAACTCTCTTGTGCACA+CGG | + | chr7.1:14732450-14732469 | None:intergenic | 45.0% | |
| GAAGAAGGGGAGTCAAAAGT+TGG | + | chr7.1:14731871-14731890 | None:intergenic | 45.0% | |
| GGCATACGTGTGAGAGTAAA+AGG | - | chr7.1:14731970-14731989 | MS.gene017955:intron | 45.0% | |
| TCCCCTTCTTCTGTGTCTAT+TGG | - | chr7.1:14731880-14731899 | MS.gene017955:intron | 45.0% | |
| TCCTGTTAGGATGCCTACTA+AGG | - | chr7.1:14731427-14731446 | MS.gene017955:intron | 45.0% | |
| TGATTCTCTTCTATGCGCAC+TGG | - | chr7.1:14732184-14732203 | MS.gene017955:intron | 45.0% | |
| TGTTACAGGTACCAACACCT+GGG | - | chr7.1:14732411-14732430 | MS.gene017955:intron | 45.0% | |
| TTAGAGTGAAGGGTCCTGTT+AGG | - | chr7.1:14731414-14731433 | MS.gene017955:intron | 45.0% | |
| TTGTTACAGGTACCAACACC+TGG | - | chr7.1:14732410-14732429 | MS.gene017955:intron | 45.0% | |
| !! | CCATAAGCAGGCTAGTTTCA+GGG | + | chr7.1:14731763-14731782 | None:intergenic | 45.0% |
| !! | CGACTACGGGTTGGAATTAA+TGG | - | chr7.1:14732118-14732137 | MS.gene017955:intron | 45.0% |
| !! | CTGTGTCTATTGGTTTGTGC+TGG | - | chr7.1:14731890-14731909 | MS.gene017955:intron | 45.0% |
| !! | GTTTGTGCTGATTTGGTTCG+TGG | - | chr7.1:14731374-14731393 | MS.gene017955:intron | 45.0% |
| !! | TCCATAAGCAGGCTAGTTTC+AGG | + | chr7.1:14731764-14731783 | None:intergenic | 45.0% |
| !! | TGATTTGGTTCGTGGTGCTA+AGG | - | chr7.1:14731382-14731401 | MS.gene017955:intron | 45.0% |
| ACCAGGTTCGATGGTGATAG+AGG | + | chr7.1:14732509-14732528 | None:intergenic | 50.0% | |
| ACCTCTATCACCATCGAACC+TGG | - | chr7.1:14732505-14732524 | MS.gene017955:CDS | 50.0% | |
| ACTACCAGGAAGACTCCTTG+TGG | - | chr7.1:14731461-14731480 | MS.gene017955:intron | 50.0% | |
| AGGAAGACTCCTTGTGGAGA+AGG | - | chr7.1:14731467-14731486 | MS.gene017955:intron | 50.0% | |
| CAACATCTGGGGAACTGTAG+AGG | + | chr7.1:14732474-14732493 | None:intergenic | 50.0% | |
| TCTTGGGATTCCTCCAAACC+AGG | + | chr7.1:14731208-14731227 | None:intergenic | 50.0% | |
| TTCTCCACAAGGAGTCTTCC+TGG | + | chr7.1:14731468-14731487 | None:intergenic | 50.0% | |
| ! | CCCTGAAACTAGCCTGCTTA+TGG | - | chr7.1:14731760-14731779 | MS.gene017955:intron | 50.0% |
| !! | TTCTATGCGCACTGGTTGCA+AGG | - | chr7.1:14732192-14732211 | MS.gene017955:intron | 50.0% |
| AACCTCGACACCAGGTTCGA+TGG | + | chr7.1:14732518-14732537 | None:intergenic | 55.0% | |
| GAAACCCACCAAGCCTGGTT+TGG | - | chr7.1:14731192-14731211 | MS.gene017955:CDS | 55.0% | |
| GGATTCCTCCAAACCAGGCT+TGG | + | chr7.1:14731203-14731222 | None:intergenic | 55.0% | |
| GTAACCTCAACCTCGACACC+AGG | + | chr7.1:14732526-14732545 | None:intergenic | 55.0% | |
| TGAAGGACTCGACTACGGGT+TGG | - | chr7.1:14732109-14732128 | MS.gene017955:intron | 55.0% | |
| TTCCTCCAAACCAGGCTTGG+TGG | + | chr7.1:14731200-14731219 | None:intergenic | 55.0% | |
| ! | TCCTCCAAACCAGGCTTGGT+GGG | + | chr7.1:14731199-14731218 | None:intergenic | 55.0% |
| GCGATGAAACCCACCAAGCC+TGG | - | chr7.1:14731187-14731206 | MS.gene017955:CDS | 60.0% | |
| ! | CACCATCGAACCTGGTGTCG+AGG | - | chr7.1:14732513-14732532 | MS.gene017955:CDS | 60.0% |
| ! | CGAACCTGGTGTCGAGGTTG+AGG | - | chr7.1:14732519-14732538 | MS.gene017955:CDS | 60.0% |
| !! | ACCCACCAAGCCTGGTTTGG+AGG | - | chr7.1:14731195-14731214 | MS.gene017955:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 14731175 | 14732561 | 14731175 | ID=MS.gene017955 |
| chr7.1 | mRNA | 14731175 | 14732561 | 14731175 | ID=MS.gene017955.t1;Parent=MS.gene017955 |
| chr7.1 | exon | 14732450 | 14732561 | 14732450 | ID=MS.gene017955.t1.exon1;Parent=MS.gene017955.t1 |
| chr7.1 | CDS | 14732450 | 14732561 | 14732450 | ID=cds.MS.gene017955.t1;Parent=MS.gene017955.t1 |
| chr7.1 | exon | 14732248 | 14732361 | 14732248 | ID=MS.gene017955.t1.exon2;Parent=MS.gene017955.t1 |
| chr7.1 | CDS | 14732248 | 14732361 | 14732248 | ID=cds.MS.gene017955.t1;Parent=MS.gene017955.t1 |
| chr7.1 | exon | 14731175 | 14731317 | 14731175 | ID=MS.gene017955.t1.exon3;Parent=MS.gene017955.t1 |
| chr7.1 | CDS | 14731175 | 14731317 | 14731175 | ID=cds.MS.gene017955.t1;Parent=MS.gene017955.t1 |
| Gene Sequence |
| Protein sequence |
