Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78060.t1 | XP_003605577.1 | 100 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.40E-61 | 245.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78060.t1 | Q9STY6 | 88.5 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 4.9e-55 | 214.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78060.t1 | G7JPA9 | 100.0 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.0e-61 | 245.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene72479 | MS.gene78060 | PPI |
| MS.gene031439 | MS.gene78060 | PPI |
| MS.gene072375 | MS.gene78060 | PPI |
| MS.gene059832 | MS.gene78060 | PPI |
| MS.gene068750 | MS.gene78060 | PPI |
| MS.gene045681 | MS.gene78060 | PPI |
| MS.gene04591 | MS.gene78060 | PPI |
| MS.gene77739 | MS.gene78060 | PPI |
| MS.gene017955 | MS.gene78060 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78060.t1 | MTR_0157s0070 | 100.000 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 4.56e-88 | 251 |
| MS.gene78060.t1 | MTR_4g034030 | 100.000 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 4.56e-88 | 251 |
| MS.gene78060.t1 | MTR_7g092720 | 98.361 | 122 | 2 | 0 | 1 | 122 | 1 | 122 | 7.09e-87 | 248 |
| MS.gene78060.t1 | MTR_2g085090 | 97.500 | 120 | 3 | 0 | 3 | 122 | 4 | 123 | 6.20e-84 | 241 |
| MS.gene78060.t1 | MTR_1g091057 | 97.500 | 120 | 3 | 0 | 3 | 122 | 4 | 123 | 6.20e-84 | 241 |
| MS.gene78060.t1 | MTR_4g034030 | 100.000 | 85 | 0 | 0 | 38 | 122 | 3 | 87 | 1.15e-58 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78060.t1 | AT3G47370 | 88.525 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 2.90e-76 | 221 |
| MS.gene78060.t1 | AT3G47370 | 88.525 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 2.90e-76 | 221 |
| MS.gene78060.t1 | AT3G47370 | 88.525 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 2.90e-76 | 221 |
| MS.gene78060.t1 | AT5G62300 | 88.430 | 121 | 14 | 0 | 2 | 122 | 4 | 124 | 2.75e-75 | 219 |
| MS.gene78060.t1 | AT5G62300 | 88.430 | 121 | 14 | 0 | 2 | 122 | 4 | 124 | 2.75e-75 | 219 |
| MS.gene78060.t1 | AT3G45030 | 88.430 | 121 | 14 | 0 | 2 | 122 | 4 | 124 | 2.75e-75 | 219 |
Find 30 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGCTAAGGATAAGCATCTT+AGG | 0.267467 | 4.1:-64820845 | MS.gene78060:CDS |
| AGAGAGTGATCCTAATCTTA+TGG | 0.362238 | 4.1:+64821012 | None:intergenic |
| TCTTCCAAACCAGGCTTAGT+TGG | 0.410878 | 4.1:+64821050 | None:intergenic |
| GCCTGGTTTGGAAGAATCTC+AGG | 0.435840 | 4.1:-64821042 | MS.gene78060:CDS |
| GAAACCAACTAAGCCTGGTT+TGG | 0.438912 | 4.1:-64821054 | MS.gene78060:CDS |
| AGGAACAGATCCATAAGATT+AGG | 0.460004 | 4.1:-64821022 | MS.gene78060:CDS |
| GTAATCTGCTTGACAACATC+AGG | 0.478418 | 4.1:+64819118 | None:intergenic |
| ACATGTTCAGAATCTCGAAA+AGG | 0.489111 | 4.1:-64820982 | MS.gene78060:CDS |
| TCCTGAGATTCTTCCAAACC+AGG | 0.490218 | 4.1:+64821041 | None:intergenic |
| AGGAAAACCCCTTGTGGAGA+AGG | 0.505939 | 4.1:-64820774 | MS.gene78060:intron |
| CATGTTCAGAATCTCGAAAA+GGG | 0.517875 | 4.1:-64820981 | MS.gene78060:intron |
| TGCTAAGGATAAGCATCTTA+GGG | 0.525206 | 4.1:-64820844 | MS.gene78060:CDS |
| TGAACCCGGTGTTGAAGTTG+AGG | 0.528118 | 4.1:-64819083 | MS.gene78060:CDS |
| TATGGTAGTTTGTAGTGACT+TGG | 0.528686 | 4.1:-64820874 | MS.gene78060:intron |
| GGTAACCTCAACTTCAACAC+CGG | 0.532891 | 4.1:+64819078 | None:intergenic |
| GCAATGAAACCAACTAAGCC+TGG | 0.533074 | 4.1:-64821059 | MS.gene78060:CDS |
| TGACTTGGTTCGTGGTGCTA+AGG | 0.539891 | 4.1:-64820859 | MS.gene78060:CDS |
| GGATAAGCATCTTAGGGTGA+AGG | 0.543022 | 4.1:-64820838 | MS.gene78060:CDS |
| GTTTGTAGTGACTTGGTTCG+TGG | 0.553261 | 4.1:-64820867 | MS.gene78060:CDS |
| TGATAATCAAGCATCTGCAA+TGG | 0.568022 | 4.1:+64819057 | None:intergenic |
| AAAATTACCTTCTCCACAAG+GGG | 0.574438 | 4.1:+64820767 | None:intergenic |
| GATAAGCATCTTAGGGTGAA+GGG | 0.583411 | 4.1:-64820837 | MS.gene78060:CDS |
| CAACATCAGGTGAACTGTAG+AGG | 0.586753 | 4.1:+64819131 | None:intergenic |
| ATGGTTAAGGTACAAACACA+TGG | 0.591038 | 4.1:-64819192 | MS.gene78060:intron |
| GTAACCTCAACTTCAACACC+GGG | 0.633780 | 4.1:+64819079 | None:intergenic |
| AAGTGCTTCACATTACCACT+AGG | 0.639472 | 4.1:-64820794 | MS.gene78060:CDS |
| ACCACTAGGAAAACCCCTTG+TGG | 0.655241 | 4.1:-64820780 | MS.gene78060:CDS |
| ACATCGATCACAATTGAACC+CGG | 0.686873 | 4.1:-64819097 | MS.gene78060:CDS |
| TGGTTAAGGTACAAACACAT+GGG | 0.717038 | 4.1:-64819191 | MS.gene78060:intron |
| TTAGGGTGAAGGGACCAGTG+AGG | 0.764206 | 4.1:-64820827 | MS.gene78060:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATAAGACAATCAAAA+CGG | + | chr4.1:64820280-64820299 | None:intergenic | 15.0% |
| !! | AATAATTATAACATAAACAC+TGG | + | chr4.1:64819929-64819948 | None:intergenic | 15.0% |
| !!! | ATTTTTCAAATAAACACTAA+GGG | + | chr4.1:64820495-64820514 | None:intergenic | 15.0% |
| !!! | TATAAGCTTTTTTTATATGA+TGG | - | chr4.1:64819200-64819219 | MS.gene78060:intron | 15.0% |
| !!! | TTTAGAATAATCTTATGTTA+TGG | + | chr4.1:64820724-64820743 | None:intergenic | 15.0% |
| !!! | TTTTTTGTTTTGTTTTGTTA+TGG | - | chr4.1:64819242-64819261 | MS.gene78060:intron | 15.0% |
| !! | ATAGTTGCATATTTCAAATT+GGG | - | chr4.1:64819971-64819990 | MS.gene78060:intron | 20.0% |
| !! | GCATGATATAAATAATTACA+AGG | - | chr4.1:64819791-64819810 | MS.gene78060:intron | 20.0% |
| !! | TATTATTCATAAGTTTGTCA+AGG | + | chr4.1:64820406-64820425 | None:intergenic | 20.0% |
| !!! | AGATATTAAGTTTGGTATTT+TGG | - | chr4.1:64819694-64819713 | MS.gene78060:intron | 20.0% |
| !!! | CATTTTTCAAATAAACACTA+AGG | + | chr4.1:64820496-64820515 | None:intergenic | 20.0% |
| ! | AAATATGCAACTATCTAAGA+TGG | + | chr4.1:64819965-64819984 | None:intergenic | 25.0% |
| ! | ACACATTCAATCATAAGTAT+CGG | + | chr4.1:64819558-64819577 | None:intergenic | 25.0% |
| ! | ATGAAGCACAAACAAAAATA+CGG | - | chr4.1:64820072-64820091 | MS.gene78060:intron | 25.0% |
| ! | GATAGTTGCATATTTCAAAT+TGG | - | chr4.1:64819970-64819989 | MS.gene78060:intron | 25.0% |
| ! | TAATTATGTTACGATCATAG+TGG | - | chr4.1:64819631-64819650 | MS.gene78060:intron | 25.0% |
| ! | TCTTTCAAACACTTATACAT+AGG | - | chr4.1:64820695-64820714 | MS.gene78060:intron | 25.0% |
| ! | TGAAATATCATTTGATGTCT+TGG | - | chr4.1:64820242-64820261 | MS.gene78060:intron | 25.0% |
| ! | TTAAACAGATTGTCAACAAA+AGG | + | chr4.1:64820923-64820942 | None:intergenic | 25.0% |
| ! | TTTGTTGACAATCTGTTTAA+TGG | - | chr4.1:64820923-64820942 | MS.gene78060:intron | 25.0% |
| !!! | ATTTTTGTTTGTGCTTCATA+TGG | + | chr4.1:64820071-64820090 | None:intergenic | 25.0% |
| !!! | CTCTCTTCTGAAATAAAATT+TGG | + | chr4.1:64820528-64820547 | None:intergenic | 25.0% |
| !!! | TTGCATTCTAAAGTAGAATT+TGG | - | chr4.1:64820587-64820606 | MS.gene78060:intron | 25.0% |
| !!! | TTTTGAAATTATGAGTTTGC+TGG | - | chr4.1:64819894-64819913 | MS.gene78060:intron | 25.0% |
| !!! | TTTTGTTTCTTTGTGCATTT+TGG | - | chr4.1:64819407-64819426 | MS.gene78060:intron | 25.0% |
| AAAAATTACCTTCTCCACAA+GGG | + | chr4.1:64819371-64819390 | None:intergenic | 30.0% | |
| AACACAAACACTTCTAATCA+GGG | + | chr4.1:64820197-64820216 | None:intergenic | 30.0% | |
| AGAATTTGGAAAGAGGTTAT+TGG | - | chr4.1:64820601-64820620 | MS.gene78060:intron | 30.0% | |
| CACATTCAATCATAAGTATC+GGG | + | chr4.1:64819557-64819576 | None:intergenic | 30.0% | |
| CTAAAGTAGAATTTGGAAAG+AGG | - | chr4.1:64820594-64820613 | MS.gene78060:intron | 30.0% | |
| GAATTTGGAAAGAGGTTATT+GGG | - | chr4.1:64820602-64820621 | MS.gene78060:intron | 30.0% | |
| GACAATCTGTTTAATGGTTA+AGG | - | chr4.1:64820929-64820948 | MS.gene78060:intron | 30.0% | |
| GACAGTCTAGATATTAAGTT+TGG | - | chr4.1:64819686-64819705 | MS.gene78060:intron | 30.0% | |
| TAAAAATTACCTTCTCCACA+AGG | + | chr4.1:64819372-64819391 | None:intergenic | 30.0% | |
| TAACACAAACACTTCTAATC+AGG | + | chr4.1:64820198-64820217 | None:intergenic | 30.0% | |
| ! | TACATTGAAATTGCGTTGAT+TGG | - | chr4.1:64820216-64820235 | MS.gene78060:intron | 30.0% |
| ! | TTTGATTTAGTGAGATACTG+TGG | - | chr4.1:64820001-64820020 | MS.gene78060:intron | 30.0% |
| AAAATTACCTTCTCCACAAG+GGG | + | chr4.1:64819370-64819389 | None:intergenic | 35.0% | |
| ACACAAACACTTCTAATCAG+GGG | + | chr4.1:64820196-64820215 | None:intergenic | 35.0% | |
| ACATGTTCAGAATCTCGAAA+AGG | - | chr4.1:64819152-64819171 | MS.gene78060:CDS | 35.0% | |
| ACTCAAACATGACACTAACA+CGG | + | chr4.1:64820143-64820162 | None:intergenic | 35.0% | |
| AGAGAGTGATCCTAATCTTA+TGG | + | chr4.1:64819125-64819144 | None:intergenic | 35.0% | |
| AGGAACAGATCCATAAGATT+AGG | - | chr4.1:64819112-64819131 | MS.gene78060:CDS | 35.0% | |
| ATAAACACTAAGGGTCTGTT+TGG | + | chr4.1:64820486-64820505 | None:intergenic | 35.0% | |
| ATCAGCTTCATGAACTATTG+CGG | - | chr4.1:64820869-64820888 | MS.gene78060:CDS | 35.0% | |
| ATGGTTAAGGTACAAACACA+TGG | - | chr4.1:64820942-64820961 | MS.gene78060:intron | 35.0% | |
| ATTAAGAACTGCACCTAGAA+AGG | + | chr4.1:64820766-64820785 | None:intergenic | 35.0% | |
| CATGTTCAGAATCTCGAAAA+GGG | - | chr4.1:64819153-64819172 | MS.gene78060:CDS | 35.0% | |
| TGCTAAGGATAAGCATCTTA+GGG | - | chr4.1:64819290-64819309 | MS.gene78060:intron | 35.0% | |
| TGGTTAAGGTACAAACACAT+GGG | - | chr4.1:64820943-64820962 | MS.gene78060:intron | 35.0% | |
| TGTTAGTGTCATGTTTGAGT+CGG | - | chr4.1:64820143-64820162 | MS.gene78060:intron | 35.0% | |
| TTAAGAACTGCACCTAGAAA+GGG | + | chr4.1:64820765-64820784 | None:intergenic | 35.0% | |
| ! | ATTTGAAACACTCCCTTTCT+AGG | - | chr4.1:64820750-64820769 | MS.gene78060:intron | 35.0% |
| ! | TATGGTAGTTTGTAGTGACT+TGG | - | chr4.1:64819260-64819279 | MS.gene78060:intron | 35.0% |
| !! | GTAATGTGAAGCACTTTTGT+AGG | + | chr4.1:64819336-64819355 | None:intergenic | 35.0% |
| !! | TAGAAAGGGAGTGTTTCAAA+TGG | + | chr4.1:64820751-64820770 | None:intergenic | 35.0% |
| AAGTGCTTCACATTACCACT+AGG | - | chr4.1:64819340-64819359 | MS.gene78060:intron | 40.0% | |
| ACATCGATCACAATTGAACC+CGG | - | chr4.1:64821037-64821056 | MS.gene78060:CDS | 40.0% | |
| CATTTACGCAATCAACGCTT+TGG | + | chr4.1:64819772-64819791 | None:intergenic | 40.0% | |
| CTCAAACATGACACTAACAC+GGG | + | chr4.1:64820142-64820161 | None:intergenic | 40.0% | |
| GATAAGCATCTTAGGGTGAA+GGG | - | chr4.1:64819297-64819316 | MS.gene78060:intron | 40.0% | |
| GGTTGACGCTTACATGAAAT+CGG | + | chr4.1:64819536-64819555 | None:intergenic | 40.0% | |
| GTAATCTGCTTGACAACATC+AGG | + | chr4.1:64821019-64821038 | None:intergenic | 40.0% | |
| GTGCTAAGGATAAGCATCTT+AGG | - | chr4.1:64819289-64819308 | MS.gene78060:intron | 40.0% | |
| GTTGACGCTTACATGAAATC+GGG | + | chr4.1:64819535-64819554 | None:intergenic | 40.0% | |
| TCAGCTTCATGAACTATTGC+GGG | - | chr4.1:64820870-64820889 | MS.gene78060:intron | 40.0% | |
| !! | GTGGTTCTAGTGATGTACTA+GGG | - | chr4.1:64820034-64820053 | MS.gene78060:intron | 40.0% |
| !!! | TCTTTGTGCATTTTGGTTGC+TGG | - | chr4.1:64819414-64819433 | MS.gene78060:intron | 40.0% |
| ACTGTCCGATCTGAATCCAA+CGG | - | chr4.1:64819743-64819762 | MS.gene78060:intron | 45.0% | |
| CAACATCAGGTGAACTGTAG+AGG | + | chr4.1:64821006-64821025 | None:intergenic | 45.0% | |
| CACTAAGGGTCTGTTTGGAT+TGG | + | chr4.1:64820481-64820500 | None:intergenic | 45.0% | |
| GAAACCAACTAAGCCTGGTT+TGG | - | chr4.1:64819080-64819099 | MS.gene78060:CDS | 45.0% | |
| GATCATAGTGGTCAGCACAT+CGG | - | chr4.1:64819643-64819662 | MS.gene78060:intron | 45.0% | |
| GCAATGAAACCAACTAAGCC+TGG | - | chr4.1:64819075-64819094 | MS.gene78060:CDS | 45.0% | |
| GGATAAGCATCTTAGGGTGA+AGG | - | chr4.1:64819296-64819315 | MS.gene78060:intron | 45.0% | |
| GGTAACCTCAACTTCAACAC+CGG | + | chr4.1:64821059-64821078 | None:intergenic | 45.0% | |
| GTAACCTCAACTTCAACACC+GGG | + | chr4.1:64821058-64821077 | None:intergenic | 45.0% | |
| GTGTTGTCAGACACTAGTGT+CGG | + | chr4.1:64819590-64819609 | None:intergenic | 45.0% | |
| TCCTGAGATTCTTCCAAACC+AGG | + | chr4.1:64819096-64819115 | None:intergenic | 45.0% | |
| TCTTCCAAACCAGGCTTAGT+TGG | + | chr4.1:64819087-64819106 | None:intergenic | 45.0% | |
| TGTTGTCAGACACTAGTGTC+GGG | + | chr4.1:64819589-64819608 | None:intergenic | 45.0% | |
| ! | ACTTTTGTAGGCATCCTCAC+TGG | + | chr4.1:64819324-64819343 | None:intergenic | 45.0% |
| ! | ATACTGTGGTTGCTTTCTGG+TGG | - | chr4.1:64820015-64820034 | MS.gene78060:intron | 45.0% |
| ! | ATCTAAGATGGACAGTCGCA+AGG | + | chr4.1:64819953-64819972 | None:intergenic | 45.0% |
| ! | GAGATACTGTGGTTGCTTTC+TGG | - | chr4.1:64820012-64820031 | MS.gene78060:intron | 45.0% |
| !! | GGTGGTTCTAGTGATGTACT+AGG | - | chr4.1:64820033-64820052 | MS.gene78060:intron | 45.0% |
| !! | GTTTGTAGTGACTTGGTTCG+TGG | - | chr4.1:64819267-64819286 | MS.gene78060:intron | 45.0% |
| !!! | AATTTTTATTTTATTTGATT+TGG | - | chr4.1:64819384-64819403 | MS.gene78060:intron | 5.0% |
| ACCACTAGGAAAACCCCTTG+TGG | - | chr4.1:64819354-64819373 | MS.gene78060:intron | 50.0% | |
| AGGAAAACCCCTTGTGGAGA+AGG | - | chr4.1:64819360-64819379 | MS.gene78060:intron | 50.0% | |
| ATCAACGCTTTGGTACCCGT+TGG | + | chr4.1:64819762-64819781 | None:intergenic | 50.0% | |
| CTGTCCGATCTGAATCCAAC+GGG | - | chr4.1:64819744-64819763 | MS.gene78060:intron | 50.0% | |
| GGTACCCGTTGGATTCAGAT+CGG | + | chr4.1:64819751-64819770 | None:intergenic | 50.0% | |
| TCAGCACATCGGTGACTGTT+GGG | - | chr4.1:64819654-64819673 | MS.gene78060:intron | 50.0% | |
| ! | TCCACAAGGGGTTTTCCTAG+TGG | + | chr4.1:64819358-64819377 | None:intergenic | 50.0% |
| ! | TGAACCCGGTGTTGAAGTTG+AGG | - | chr4.1:64821051-64821070 | MS.gene78060:CDS | 50.0% |
| !! | GCCTGGTTTGGAAGAATCTC+AGG | - | chr4.1:64819092-64819111 | MS.gene78060:CDS | 50.0% |
| !! | TGACTTGGTTCGTGGTGCTA+AGG | - | chr4.1:64819275-64819294 | MS.gene78060:intron | 50.0% |
| GTCAGCACATCGGTGACTGT+TGG | - | chr4.1:64819653-64819672 | MS.gene78060:intron | 55.0% | |
| TTAGGGTGAAGGGACCAGTG+AGG | - | chr4.1:64819307-64819326 | MS.gene78060:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 64819063 | 64821093 | 64819063 | ID=MS.gene78060 |
| chr4.1 | mRNA | 64819063 | 64821093 | 64819063 | ID=MS.gene78060.t1;Parent=MS.gene78060 |
| chr4.1 | exon | 64820982 | 64821093 | 64820982 | ID=MS.gene78060.t1.exon1;Parent=MS.gene78060.t1 |
| chr4.1 | CDS | 64820982 | 64821093 | 64820982 | ID=cds.MS.gene78060.t1;Parent=MS.gene78060.t1 |
| chr4.1 | exon | 64820775 | 64820888 | 64820775 | ID=MS.gene78060.t1.exon2;Parent=MS.gene78060.t1 |
| chr4.1 | CDS | 64820775 | 64820888 | 64820775 | ID=cds.MS.gene78060.t1;Parent=MS.gene78060.t1 |
| chr4.1 | exon | 64819063 | 64819205 | 64819063 | ID=MS.gene78060.t1.exon3;Parent=MS.gene78060.t1 |
| chr4.1 | CDS | 64819063 | 64819205 | 64819063 | ID=cds.MS.gene78060.t1;Parent=MS.gene78060.t1 |
| Gene Sequence |
| Protein sequence |
