Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04591.t1 | XP_003625216.1 | 100 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.40E-61 | 245.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04591.t1 | Q9STY6 | 89.3 | 122 | 13 | 0 | 1 | 122 | 1 | 122 | 2.2e-55 | 216.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04591.t1 | A2Q4V6 | 100.0 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.0e-61 | 245.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045681 | MS.gene04591 | PPI |
| MS.gene072375 | MS.gene04591 | PPI |
| MS.gene04591 | MS.gene059832 | PPI |
| MS.gene017955 | MS.gene04591 | PPI |
| MS.gene72479 | MS.gene04591 | PPI |
| MS.gene04591 | MS.gene77739 | PPI |
| MS.gene031439 | MS.gene04591 | PPI |
| MS.gene04591 | MS.gene78060 | PPI |
| MS.gene04591 | MS.gene072375 | PPI |
| MS.gene068750 | MS.gene04591 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04591.t1 | MTR_7g092720 | 100.000 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.11e-87 | 250 |
| MS.gene04591.t1 | MTR_0157s0070 | 98.361 | 122 | 2 | 0 | 1 | 122 | 1 | 122 | 7.09e-87 | 248 |
| MS.gene04591.t1 | MTR_4g034030 | 98.361 | 122 | 2 | 0 | 1 | 122 | 1 | 122 | 7.09e-87 | 248 |
| MS.gene04591.t1 | MTR_2g085090 | 97.500 | 120 | 3 | 0 | 3 | 122 | 4 | 123 | 9.83e-84 | 240 |
| MS.gene04591.t1 | MTR_1g091057 | 97.500 | 120 | 3 | 0 | 3 | 122 | 4 | 123 | 9.83e-84 | 240 |
| MS.gene04591.t1 | MTR_4g034030 | 98.824 | 85 | 1 | 0 | 38 | 122 | 3 | 87 | 3.02e-58 | 174 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04591.t1 | AT3G47370 | 89.344 | 122 | 13 | 0 | 1 | 122 | 1 | 122 | 2.23e-76 | 222 |
| MS.gene04591.t1 | AT3G47370 | 89.344 | 122 | 13 | 0 | 1 | 122 | 1 | 122 | 2.23e-76 | 222 |
| MS.gene04591.t1 | AT3G47370 | 89.344 | 122 | 13 | 0 | 1 | 122 | 1 | 122 | 2.23e-76 | 222 |
| MS.gene04591.t1 | AT5G62300 | 89.256 | 121 | 13 | 0 | 2 | 122 | 4 | 124 | 1.92e-75 | 219 |
| MS.gene04591.t1 | AT5G62300 | 89.256 | 121 | 13 | 0 | 2 | 122 | 4 | 124 | 1.92e-75 | 219 |
| MS.gene04591.t1 | AT3G45030 | 89.256 | 121 | 13 | 0 | 2 | 122 | 4 | 124 | 1.92e-75 | 219 |
Find 42 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATTAGTTTGTGCTGATT+TGG | 0.351954 | 7.3:-61800063 | MS.gene04591:intron |
| AAAGGGTGATCCTAATCTTA+TGG | 0.356442 | 7.3:+61800196 | None:intergenic |
| TTCTCCACAAGGAGTCTTCC+TGG | 0.359877 | 7.3:+61799965 | None:intergenic |
| GTAATCTGTTTAACAACATC+TGG | 0.381438 | 7.3:+61798948 | None:intergenic |
| AATCTTATGGATCTGCTCTT+GGG | 0.403761 | 7.3:+61800209 | None:intergenic |
| TAATCTTATGGATCTGCTCT+TGG | 0.405883 | 7.3:+61800208 | None:intergenic |
| GGATTCCTCCAAACCAGGCT+TGG | 0.415376 | 7.3:+61800230 | None:intergenic |
| GAAACCCACCAAGCCTGGTT+TGG | 0.420702 | 7.3:-61800238 | MS.gene04591:CDS |
| TCCTCCAAACCAGGCTTGGT+GGG | 0.429749 | 7.3:+61800234 | None:intergenic |
| TTCCTCCAAACCAGGCTTGG+TGG | 0.433644 | 7.3:+61800233 | None:intergenic |
| GTGATATGAAGAACCTTAGT+AGG | 0.436083 | 7.3:+61799990 | None:intergenic |
| TTAGAGTGAAGGGTCCTGTT+AGG | 0.436691 | 7.3:-61800016 | MS.gene04591:CDS |
| ACCTTAGTAGGCATCCTAAC+AGG | 0.444549 | 7.3:+61800002 | None:intergenic |
| AAGAGCAGATCCATAAGATT+AGG | 0.460004 | 7.3:-61800206 | MS.gene04591:CDS |
| TTGTTACAGGTACCAACACC+TGG | 0.473935 | 7.3:-61799022 | MS.gene04591:intron |
| TTTGACATGCTTGGAGGAAA+GGG | 0.476236 | 7.3:+61800179 | None:intergenic |
| ACCAGGTTCGATGGTGATAG+AGG | 0.489959 | 7.3:+61798926 | None:intergenic |
| GTTTGTGCTGATTTGGTTCG+TGG | 0.496204 | 7.3:-61800056 | MS.gene04591:CDS |
| GCGATGAAACCCACCAAGCC+TGG | 0.496657 | 7.3:-61800243 | MS.gene04591:CDS |
| CGAACCTGGTGTCGAGGTTG+AGG | 0.501443 | 7.3:-61798913 | MS.gene04591:CDS |
| AGGAAGACTCCTTGTGGAGA+AGG | 0.508893 | 7.3:-61799963 | MS.gene04591:intron |
| TCCTGTTAGGATGCCTACTA+AGG | 0.508963 | 7.3:-61800003 | MS.gene04591:CDS |
| ACCCACCAAGCCTGGTTTGG+AGG | 0.512859 | 7.3:-61800235 | MS.gene04591:CDS |
| TCTTGGGATTCCTCCAAACC+AGG | 0.514536 | 7.3:+61800225 | None:intergenic |
| ACCTCTATCACCATCGAACC+TGG | 0.519945 | 7.3:-61798927 | MS.gene04591:CDS |
| TAATCTGTTTAACAACATCT+GGG | 0.529679 | 7.3:+61798949 | None:intergenic |
| TGATTTGGTTCGTGGCGCTA+AGG | 0.536928 | 7.3:-61800048 | MS.gene04591:CDS |
| AACCTCGACACCAGGTTCGA+TGG | 0.552766 | 7.3:+61798917 | None:intergenic |
| CACCATCGAACCTGGTGTCG+AGG | 0.559437 | 7.3:-61798919 | MS.gene04591:CDS |
| CGATAACTCTCTTGTGCACA+CGG | 0.563868 | 7.3:+61798985 | None:intergenic |
| GGATAAGCATCTTAGAGTGA+AGG | 0.564807 | 7.3:-61800027 | MS.gene04591:CDS |
| TTCAAATCTATCCCAGGTGT+TGG | 0.568966 | 7.3:+61799010 | None:intergenic |
| GATAAGCATCTTAGAGTGAA+GGG | 0.578400 | 7.3:-61800026 | MS.gene04591:CDS |
| GTAACCTCAACCTCGACACC+AGG | 0.583580 | 7.3:+61798909 | None:intergenic |
| CAACATCTGGGGAACTGTAG+AGG | 0.590461 | 7.3:+61798961 | None:intergenic |
| ACGGAGTTCAAATCTATCCC+AGG | 0.607565 | 7.3:+61799004 | None:intergenic |
| AGGTTCTTCATATCACTACC+AGG | 0.631821 | 7.3:-61799983 | MS.gene04591:CDS |
| TTAAGATCAAGCATCTGCAA+TGG | 0.639875 | 7.3:+61798887 | None:intergenic |
| AATGAAATACCTTCTCCACA+AGG | 0.646547 | 7.3:+61799954 | None:intergenic |
| ACTACCAGGAAGACTCCTTG+TGG | 0.667890 | 7.3:-61799969 | MS.gene04591:CDS |
| TGTTACAGGTACCAACACCT+GGG | 0.689167 | 7.3:-61799021 | MS.gene04591:intron |
| AATCTGTTTAACAACATCTG+GGG | 0.693040 | 7.3:+61798950 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCTCTTTAAAATAATAAAT+GGG | - | chr7.3:61799563-61799582 | MS.gene04591:intron | 10.0% |
| !!! | TTTTATAATTATTTGATGAA+AGG | - | chr7.3:61799260-61799279 | MS.gene04591:intron | 10.0% |
| !!! | GTTCTCTTTAAAATAATAAA+TGG | - | chr7.3:61799562-61799581 | MS.gene04591:intron | 15.0% |
| !!! | TTTAATTCAGTTTTGTTGAA+TGG | - | chr7.3:61799763-61799782 | MS.gene04591:intron | 20.0% |
| ! | AATTATCCTACAACCTTAAT+TGG | - | chr7.3:61799665-61799684 | MS.gene04591:intron | 25.0% |
| ! | TAATCTGTTTAACAACATCT+GGG | + | chr7.3:61800202-61800221 | None:intergenic | 25.0% |
| !! | TGAGAGCATAAAAAACATAA+AGG | + | chr7.3:61799027-61799046 | None:intergenic | 25.0% |
| !! | TTTCATTGTGTTCTATCTTT+TGG | - | chr7.3:61799637-61799656 | MS.gene04591:intron | 25.0% |
| !!! | GCATTTTGTTATTATGCTAT+TGG | - | chr7.3:61799941-61799960 | MS.gene04591:intron | 25.0% |
| !!! | TGTTTCTGAACAAATTTTGA+AGG | - | chr7.3:61799808-61799827 | MS.gene04591:intron | 25.0% |
| AATCTGTTTAACAACATCTG+GGG | + | chr7.3:61800201-61800220 | None:intergenic | 30.0% | |
| AGATTAGAAACATCATTGAG+AGG | - | chr7.3:61799298-61799317 | MS.gene04591:intron | 30.0% | |
| ATTTGATGAAAGGTAGTCAA+TGG | - | chr7.3:61799270-61799289 | MS.gene04591:intron | 30.0% | |
| GATTAGAAACATCATTGAGA+GGG | - | chr7.3:61799299-61799318 | MS.gene04591:intron | 30.0% | |
| GTAATCTGTTTAACAACATC+TGG | + | chr7.3:61800203-61800222 | None:intergenic | 30.0% | |
| TAAAGAGAACAAACCAAACA+CGG | + | chr7.3:61799552-61799571 | None:intergenic | 30.0% | |
| TCCTAATATGGCTTACAAAT+GGG | - | chr7.3:61799439-61799458 | MS.gene04591:intron | 30.0% | |
| ! | CTAGTTGTAGTTTTTGCAAT+TGG | - | chr7.3:61799596-61799615 | MS.gene04591:intron | 30.0% |
| ! | GTTTTTCTAAGCTCCTAATA+TGG | - | chr7.3:61799427-61799446 | MS.gene04591:intron | 30.0% |
| !! | GTTATTAGTTTGTGCTGATT+TGG | - | chr7.3:61799085-61799104 | MS.gene04591:intron | 30.0% |
| AAAGGGTGATCCTAATCTTA+TGG | + | chr7.3:61798955-61798974 | None:intergenic | 35.0% | |
| AAATACGATATAGCCGTGTT+TGG | - | chr7.3:61799536-61799555 | MS.gene04591:intron | 35.0% | |
| AAGAGCAGATCCATAAGATT+AGG | - | chr7.3:61798942-61798961 | MS.gene04591:CDS | 35.0% | |
| AATCTTATGGATCTGCTCTT+GGG | + | chr7.3:61798942-61798961 | None:intergenic | 35.0% | |
| AATGAAATACCTTCTCCACA+AGG | + | chr7.3:61799197-61799216 | None:intergenic | 35.0% | |
| ACCCATTTGTAAGCCATATT+AGG | + | chr7.3:61799443-61799462 | None:intergenic | 35.0% | |
| ATCCTACACTTCAATGACAA+TGG | + | chr7.3:61800048-61800067 | None:intergenic | 35.0% | |
| ATGACCTTACTTCTTGTTAC+AGG | - | chr7.3:61800113-61800132 | MS.gene04591:intron | 35.0% | |
| CATGTCAAAAATCTCGAGAA+GGG | - | chr7.3:61798983-61799002 | MS.gene04591:CDS | 35.0% | |
| CTCCTAATATGGCTTACAAA+TGG | - | chr7.3:61799438-61799457 | MS.gene04591:intron | 35.0% | |
| GATAAGCATCTTAGAGTGAA+GGG | - | chr7.3:61799122-61799141 | MS.gene04591:intron | 35.0% | |
| GCATCTAAATTTCCATAAGC+AGG | + | chr7.3:61799492-61799511 | None:intergenic | 35.0% | |
| GTGATATGAAGAACCTTAGT+AGG | + | chr7.3:61799161-61799180 | None:intergenic | 35.0% | |
| TAATCTTATGGATCTGCTCT+TGG | + | chr7.3:61798943-61798962 | None:intergenic | 35.0% | |
| TGTGCATAGAAGAGAATCAA+CGG | + | chr7.3:61799899-61799918 | None:intergenic | 35.0% | |
| !! | AATTTTGAAGGACTCGACTA+CGG | - | chr7.3:61799820-61799839 | MS.gene04591:intron | 35.0% |
| !!! | CTTGATAGTGCTATGTTTTC+TGG | - | chr7.3:61800005-61800024 | MS.gene04591:CDS | 35.0% |
| !!! | TTTTTGCAATTGGTTTGTGC+TGG | - | chr7.3:61799606-61799625 | MS.gene04591:intron | 35.0% |
| AGCCATTGTCATTGAAGTGT+AGG | - | chr7.3:61800043-61800062 | MS.gene04591:CDS | 40.0% | |
| AGGTTCTTCATATCACTACC+AGG | - | chr7.3:61799165-61799184 | MS.gene04591:intron | 40.0% | |
| CACTATCAAGTATTCAGCAG+AGG | + | chr7.3:61799995-61800014 | None:intergenic | 40.0% | |
| CGTATGCCAATTAAGGTTGT+AGG | + | chr7.3:61799674-61799693 | None:intergenic | 40.0% | |
| GCATGTCAAAAATCTCGAGA+AGG | - | chr7.3:61798982-61799001 | MS.gene04591:CDS | 40.0% | |
| GGATAAGCATCTTAGAGTGA+AGG | - | chr7.3:61799121-61799140 | MS.gene04591:intron | 40.0% | |
| GGTACCTGTAACAAGAAGTA+AGG | + | chr7.3:61800120-61800139 | None:intergenic | 40.0% | |
| TACGTGTGAGAGTAAAAGGA+AGG | - | chr7.3:61799690-61799709 | MS.gene04591:intron | 40.0% | |
| TCAAGTATTCAGCAGAGGTT+TGG | + | chr7.3:61799990-61800009 | None:intergenic | 40.0% | |
| TCTCACACGTATGCCAATTA+AGG | + | chr7.3:61799681-61799700 | None:intergenic | 40.0% | |
| TGAGAGGGAACTGTTGAAAT+AGG | - | chr7.3:61799314-61799333 | MS.gene04591:intron | 40.0% | |
| TGATTCTCTTCTATGCACAC+TGG | - | chr7.3:61799900-61799919 | MS.gene04591:intron | 40.0% | |
| TTCAAATCTATCCCAGGTGT+TGG | + | chr7.3:61800141-61800160 | None:intergenic | 40.0% | |
| TTTGACATGCTTGGAGGAAA+GGG | + | chr7.3:61798972-61798991 | None:intergenic | 40.0% | |
| ! | CTCGAGATTTTTGACATGCT+TGG | + | chr7.3:61798981-61799000 | None:intergenic | 40.0% |
| ! | GAGATTTTTGACATGCTTGG+AGG | + | chr7.3:61798978-61798997 | None:intergenic | 40.0% |
| ! | TTTTGACATGCTTGGAGGAA+AGG | + | chr7.3:61798973-61798992 | None:intergenic | 40.0% |
| !! | ATTTTGAAGGACTCGACTAC+GGG | - | chr7.3:61799821-61799840 | MS.gene04591:intron | 40.0% |
| ACCTTAGTAGGCATCCTAAC+AGG | + | chr7.3:61799149-61799168 | None:intergenic | 45.0% | |
| ACGGAGTTCAAATCTATCCC+AGG | + | chr7.3:61800147-61800166 | None:intergenic | 45.0% | |
| CGATAACTCTCTTGTGCACA+CGG | + | chr7.3:61800166-61800185 | None:intergenic | 45.0% | |
| GGCATACGTGTGAGAGTAAA+AGG | - | chr7.3:61799686-61799705 | MS.gene04591:intron | 45.0% | |
| TCCTGTTAGGATGCCTACTA+AGG | - | chr7.3:61799145-61799164 | MS.gene04591:intron | 45.0% | |
| TGTTACAGGTACCAACACCT+GGG | - | chr7.3:61800127-61800146 | MS.gene04591:intron | 45.0% | |
| TTAGAGTGAAGGGTCCTGTT+AGG | - | chr7.3:61799132-61799151 | MS.gene04591:intron | 45.0% | |
| TTGTTACAGGTACCAACACC+TGG | - | chr7.3:61800126-61800145 | MS.gene04591:intron | 45.0% | |
| ! | TTCTATGCACACTGGTTGCA+AGG | - | chr7.3:61799908-61799927 | MS.gene04591:intron | 45.0% |
| !! | CCATAAGCAGGCTAGTTTCA+GGG | + | chr7.3:61799480-61799499 | None:intergenic | 45.0% |
| !! | CGACTACGGGTTGGAATTAA+TGG | - | chr7.3:61799834-61799853 | MS.gene04591:intron | 45.0% |
| !! | GTTTGTGCTGATTTGGTTCG+TGG | - | chr7.3:61799092-61799111 | MS.gene04591:intron | 45.0% |
| !! | TCCATAAGCAGGCTAGTTTC+AGG | + | chr7.3:61799481-61799500 | None:intergenic | 45.0% |
| ACCAGGTTCGATGGTGATAG+AGG | + | chr7.3:61800225-61800244 | None:intergenic | 50.0% | |
| ACCTCTATCACCATCGAACC+TGG | - | chr7.3:61800221-61800240 | MS.gene04591:CDS | 50.0% | |
| ACTACCAGGAAGACTCCTTG+TGG | - | chr7.3:61799179-61799198 | MS.gene04591:intron | 50.0% | |
| AGGAAGACTCCTTGTGGAGA+AGG | - | chr7.3:61799185-61799204 | MS.gene04591:intron | 50.0% | |
| CAACATCTGGGGAACTGTAG+AGG | + | chr7.3:61800190-61800209 | None:intergenic | 50.0% | |
| TCTTGGGATTCCTCCAAACC+AGG | + | chr7.3:61798926-61798945 | None:intergenic | 50.0% | |
| TTCTCCACAAGGAGTCTTCC+TGG | + | chr7.3:61799186-61799205 | None:intergenic | 50.0% | |
| ! | CCCTGAAACTAGCCTGCTTA+TGG | - | chr7.3:61799477-61799496 | MS.gene04591:intron | 50.0% |
| !! | TGATTTGGTTCGTGGCGCTA+AGG | - | chr7.3:61799100-61799119 | MS.gene04591:intron | 50.0% |
| AACCTCGACACCAGGTTCGA+TGG | + | chr7.3:61800234-61800253 | None:intergenic | 55.0% | |
| GAAACCCACCAAGCCTGGTT+TGG | - | chr7.3:61798910-61798929 | MS.gene04591:CDS | 55.0% | |
| GGATTCCTCCAAACCAGGCT+TGG | + | chr7.3:61798921-61798940 | None:intergenic | 55.0% | |
| GTAACCTCAACCTCGACACC+AGG | + | chr7.3:61800242-61800261 | None:intergenic | 55.0% | |
| TGAAGGACTCGACTACGGGT+TGG | - | chr7.3:61799825-61799844 | MS.gene04591:intron | 55.0% | |
| TTCCTCCAAACCAGGCTTGG+TGG | + | chr7.3:61798918-61798937 | None:intergenic | 55.0% | |
| ! | TCCTCCAAACCAGGCTTGGT+GGG | + | chr7.3:61798917-61798936 | None:intergenic | 55.0% |
| GCGATGAAACCCACCAAGCC+TGG | - | chr7.3:61798905-61798924 | MS.gene04591:CDS | 60.0% | |
| ! | CACCATCGAACCTGGTGTCG+AGG | - | chr7.3:61800229-61800248 | MS.gene04591:CDS | 60.0% |
| ! | CGAACCTGGTGTCGAGGTTG+AGG | - | chr7.3:61800235-61800254 | MS.gene04591:CDS | 60.0% |
| !! | ACCCACCAAGCCTGGTTTGG+AGG | - | chr7.3:61798913-61798932 | MS.gene04591:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 61798893 | 61800277 | 61798893 | ID=MS.gene04591 |
| chr7.3 | mRNA | 61798893 | 61800277 | 61798893 | ID=MS.gene04591.t1;Parent=MS.gene04591 |
| chr7.3 | exon | 61800166 | 61800277 | 61800166 | ID=MS.gene04591.t1.exon1;Parent=MS.gene04591.t1 |
| chr7.3 | CDS | 61800166 | 61800277 | 61800166 | ID=cds.MS.gene04591.t1;Parent=MS.gene04591.t1 |
| chr7.3 | exon | 61799964 | 61800077 | 61799964 | ID=MS.gene04591.t1.exon2;Parent=MS.gene04591.t1 |
| chr7.3 | CDS | 61799964 | 61800077 | 61799964 | ID=cds.MS.gene04591.t1;Parent=MS.gene04591.t1 |
| chr7.3 | exon | 61798893 | 61799035 | 61798893 | ID=MS.gene04591.t1.exon3;Parent=MS.gene04591.t1 |
| chr7.3 | CDS | 61798893 | 61799035 | 61798893 | ID=cds.MS.gene04591.t1;Parent=MS.gene04591.t1 |
| Gene Sequence |
| Protein sequence |
