Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72479.t1 | XP_003605577.1 | 100 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.40E-61 | 245.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72479.t1 | Q9STY6 | 88.5 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 4.9e-55 | 214.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72479.t1 | G7JPA9 | 100.0 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.0e-61 | 245.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene72479 | MS.gene77739 | PPI |
| MS.gene72479 | MS.gene78060 | PPI |
| MS.gene72479 | MS.gene031439 | PPI |
| MS.gene72479 | MS.gene04591 | PPI |
| MS.gene72479 | MS.gene072375 | PPI |
| MS.gene72479 | MS.gene017955 | PPI |
| MS.gene045681 | MS.gene72479 | PPI |
| MS.gene72479 | MS.gene059832 | PPI |
| MS.gene72479 | MS.gene068750 | PPI |
| MS.gene068750 | MS.gene72479 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72479.t1 | MTR_0157s0070 | 100.000 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 4.56e-88 | 251 |
| MS.gene72479.t1 | MTR_4g034030 | 100.000 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 4.56e-88 | 251 |
| MS.gene72479.t1 | MTR_7g092720 | 98.361 | 122 | 2 | 0 | 1 | 122 | 1 | 122 | 7.09e-87 | 248 |
| MS.gene72479.t1 | MTR_2g085090 | 97.500 | 120 | 3 | 0 | 3 | 122 | 4 | 123 | 6.20e-84 | 241 |
| MS.gene72479.t1 | MTR_1g091057 | 97.500 | 120 | 3 | 0 | 3 | 122 | 4 | 123 | 6.20e-84 | 241 |
| MS.gene72479.t1 | MTR_4g034030 | 100.000 | 85 | 0 | 0 | 38 | 122 | 3 | 87 | 1.15e-58 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72479.t1 | AT3G47370 | 88.525 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 2.90e-76 | 221 |
| MS.gene72479.t1 | AT3G47370 | 88.525 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 2.90e-76 | 221 |
| MS.gene72479.t1 | AT3G47370 | 88.525 | 122 | 14 | 0 | 1 | 122 | 1 | 122 | 2.90e-76 | 221 |
| MS.gene72479.t1 | AT5G62300 | 88.430 | 121 | 14 | 0 | 2 | 122 | 4 | 124 | 2.75e-75 | 219 |
| MS.gene72479.t1 | AT5G62300 | 88.430 | 121 | 14 | 0 | 2 | 122 | 4 | 124 | 2.75e-75 | 219 |
| MS.gene72479.t1 | AT3G45030 | 88.430 | 121 | 14 | 0 | 2 | 122 | 4 | 124 | 2.75e-75 | 219 |
Find 31 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGCCAAGGATAAGCATCTT+AGG | 0.269950 | 4.2:-69569454 | MS.gene72479:CDS |
| AGAGAGTGATCCTAATCTTA+TGG | 0.362238 | 4.2:+69569620 | None:intergenic |
| TCTTCCAAACCAGGCTTAGT+TGG | 0.410878 | 4.2:+69569658 | None:intergenic |
| GCCTGGTTTGGAAGAATCTC+AGG | 0.435840 | 4.2:-69569650 | MS.gene72479:CDS |
| GAAACCAACTAAGCCTGGTT+TGG | 0.438912 | 4.2:-69569662 | MS.gene72479:CDS |
| AGGAACAGATCCATAAGATT+AGG | 0.460004 | 4.2:-69569630 | MS.gene72479:CDS |
| GTAATCTGCTTGACAACATC+AGG | 0.478418 | 4.2:+69567691 | None:intergenic |
| ACATGTTCAGAATCTCGAAA+AGG | 0.489111 | 4.2:-69569590 | MS.gene72479:CDS |
| TCCTGAGATTCTTCCAAACC+AGG | 0.490218 | 4.2:+69569649 | None:intergenic |
| GGTAACCTCAACTTCGACAC+CGG | 0.491925 | 4.2:+69567651 | None:intergenic |
| AGGAAAACTCCTTGTGGAGA+AGG | 0.495414 | 4.2:-69569383 | MS.gene72479:intron |
| TATTGTAGTTTGCAGTGACT+TGG | 0.503061 | 4.2:-69569483 | MS.gene72479:intron |
| CACCCTAAGATGCTTATCCT+TGG | 0.507856 | 4.2:+69569451 | None:intergenic |
| CATGTTCAGAATCTCGAAAA+GGG | 0.517875 | 4.2:-69569589 | MS.gene72479:intron |
| GCAATGAAACCAACTAAGCC+TGG | 0.533074 | 4.2:-69569667 | MS.gene72479:CDS |
| TGCCAAGGATAAGCATCTTA+GGG | 0.534887 | 4.2:-69569453 | MS.gene72479:CDS |
| GGATAAGCATCTTAGGGTGA+AGG | 0.553840 | 4.2:-69569447 | MS.gene72479:CDS |
| TGAACCCGGTGTCGAAGTTG+AGG | 0.555520 | 4.2:-69567656 | MS.gene72479:CDS |
| TGATAATCAAGCATCTGCAA+TGG | 0.568022 | 4.2:+69567630 | None:intergenic |
| GTTTGCAGTGACTTGGTTCG+TGG | 0.568396 | 4.2:-69569476 | MS.gene72479:CDS |
| TGACTTGGTTCGTGGTGCCA+AGG | 0.577464 | 4.2:-69569468 | MS.gene72479:CDS |
| CAACATCAGGTGAACTGTAG+AGG | 0.586753 | 4.2:+69567704 | None:intergenic |
| GTAACCTCAACTTCGACACC+GGG | 0.590799 | 4.2:+69567652 | None:intergenic |
| ATGGTTAAGGTACAAACACA+TGG | 0.600096 | 4.2:-69567765 | MS.gene72479:intron |
| GATAAGCATCTTAGGGTGAA+GGG | 0.610263 | 4.2:-69569446 | MS.gene72479:CDS |
| ACCACTAGGAAAACTCCTTG+TGG | 0.627419 | 4.2:-69569389 | MS.gene72479:CDS |
| TAAAATTTACCTTCTCCACA+AGG | 0.628775 | 4.2:+69569374 | None:intergenic |
| AAGTGCTTCACATTACCACT+AGG | 0.639472 | 4.2:-69569403 | MS.gene72479:CDS |
| ACATCGATCACAATTGAACC+CGG | 0.686873 | 4.2:-69567670 | MS.gene72479:CDS |
| TGGTTAAGGTACAAACACAT+GGG | 0.706931 | 4.2:-69567764 | MS.gene72479:intron |
| TTAGGGTGAAGGGACCAGTG+AGG | 0.764206 | 4.2:-69569436 | MS.gene72479:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATAAGACAATCAAAA+CGG | + | chr4.2:69568852-69568871 | None:intergenic | 15.0% |
| !!! | AAGGTAAATTTTATTTGATT+TGG | - | chr4.2:69567951-69567970 | MS.gene72479:intron | 15.0% |
| !!! | ATATGCTTTTTTTTATTTGA+TGG | - | chr4.2:69567773-69567792 | MS.gene72479:intron | 15.0% |
| !!! | ATTTTTCAAATAAACACTAA+GGG | + | chr4.2:69569067-69569086 | None:intergenic | 15.0% |
| !!! | TTTAGAATAATATTATGTCA+CGG | + | chr4.2:69569285-69569304 | None:intergenic | 15.0% |
| !! | AATAATTATGACATAAACAC+TGG | + | chr4.2:69568501-69568520 | None:intergenic | 20.0% |
| !! | ATAGTTGCATATTTCAAATT+GGG | - | chr4.2:69568543-69568562 | MS.gene72479:intron | 20.0% |
| !! | GCATGATATAAATAATTACA+AGG | - | chr4.2:69568362-69568381 | MS.gene72479:intron | 20.0% |
| !! | TATTATTCATAAGTTTGTCA+AGG | + | chr4.2:69568978-69568997 | None:intergenic | 20.0% |
| !!! | AGATATTAAGTTTGGTATTT+TGG | - | chr4.2:69568265-69568284 | MS.gene72479:intron | 20.0% |
| !!! | CATTTTTCAAATAAACACTA+AGG | + | chr4.2:69569068-69569087 | None:intergenic | 20.0% |
| !!! | TCTGCTTTTATTTTTAACAA+TGG | - | chr4.2:69569369-69569388 | MS.gene72479:intron | 20.0% |
| ! | AAATATGCAACTATCTAAGA+TGG | + | chr4.2:69568537-69568556 | None:intergenic | 25.0% |
| ! | ATTAAGAACTGCATTTAGAA+AGG | + | chr4.2:69569327-69569346 | None:intergenic | 25.0% |
| ! | GATAGTTGCATATTTCAAAT+TGG | - | chr4.2:69568542-69568561 | MS.gene72479:intron | 25.0% |
| ! | TGAAATATCATTTGATGTCT+TGG | - | chr4.2:69568814-69568833 | MS.gene72479:intron | 25.0% |
| ! | TTAAGAACTGCATTTAGAAA+GGG | + | chr4.2:69569326-69569345 | None:intergenic | 25.0% |
| !!! | ATTTTTGTTTGTGCTTCATA+TGG | + | chr4.2:69568643-69568662 | None:intergenic | 25.0% |
| !!! | CTCTCTTCTGAAATAAAATT+TGG | + | chr4.2:69569100-69569119 | None:intergenic | 25.0% |
| !!! | TGCATTCTAAAGTAGAATTT+GGG | - | chr4.2:69569160-69569179 | MS.gene72479:intron | 25.0% |
| !!! | TTGCATTCTAAAGTAGAATT+TGG | - | chr4.2:69569159-69569178 | MS.gene72479:intron | 25.0% |
| !!! | TTTTGAAATTATGAGTTTGC+TGG | - | chr4.2:69568466-69568485 | MS.gene72479:intron | 25.0% |
| !!! | TTTTTTTCCTTTGTGCATTT+TGG | - | chr4.2:69567977-69567996 | MS.gene72479:intron | 25.0% |
| AAAATACCCGAATTAGTTCA+TGG | + | chr4.2:69569409-69569428 | None:intergenic | 30.0% | |
| AACACAAACACTTCTAATCA+GGG | + | chr4.2:69568769-69568788 | None:intergenic | 30.0% | |
| ACTCAAACATTACACTAACA+CGG | + | chr4.2:69568715-69568734 | None:intergenic | 30.0% | |
| GAATTATGTTACGATCATAG+TGG | - | chr4.2:69568217-69568236 | MS.gene72479:intron | 30.0% | |
| GACAGTCTAGATATTAAGTT+TGG | - | chr4.2:69568257-69568276 | MS.gene72479:intron | 30.0% | |
| TAAAATTTACCTTCTCCACA+AGG | + | chr4.2:69567944-69567963 | None:intergenic | 30.0% | |
| TAAAGTAGAATTTGGGAAGA+GGG | - | chr4.2:69569167-69569186 | MS.gene72479:intron | 30.0% | |
| TAACACAAACACTTCTAATC+AGG | + | chr4.2:69568770-69568789 | None:intergenic | 30.0% | |
| TCATGTCATGAAAATAGTTG+CGG | + | chr4.2:69569469-69569488 | None:intergenic | 30.0% | |
| TGAAACAGATTGTCAATACA+AGG | + | chr4.2:69569531-69569550 | None:intergenic | 30.0% | |
| TGTTAGTGTAATGTTTGAGT+CGG | - | chr4.2:69568715-69568734 | MS.gene72479:intron | 30.0% | |
| ! | TGTATTGACAATCTGTTTCA+TGG | - | chr4.2:69569531-69569550 | MS.gene72479:intron | 30.0% |
| ! | TTTGATTTAGTGAGATACTG+TGG | - | chr4.2:69568573-69568592 | MS.gene72479:intron | 30.0% |
| ACACAAACACTTCTAATCAG+GGG | + | chr4.2:69568768-69568787 | None:intergenic | 35.0% | |
| ACATGTTCAGAATCTCGAAA+AGG | - | chr4.2:69567725-69567744 | MS.gene72479:CDS | 35.0% | |
| AGAGAGTGATCCTAATCTTA+TGG | + | chr4.2:69567698-69567717 | None:intergenic | 35.0% | |
| AGGAACAGATCCATAAGATT+AGG | - | chr4.2:69567685-69567704 | MS.gene72479:CDS | 35.0% | |
| ATAAACACTAAGGGTCTGTT+TGG | + | chr4.2:69569058-69569077 | None:intergenic | 35.0% | |
| ATCAGCTCCATGAACTAATT+CGG | - | chr4.2:69569399-69569418 | MS.gene72479:CDS | 35.0% | |
| ATGGTTAAGGTACAAACACA+TGG | - | chr4.2:69569550-69569569 | MS.gene72479:intron | 35.0% | |
| CATGTCATGAAAATAGTTGC+GGG | + | chr4.2:69569468-69569487 | None:intergenic | 35.0% | |
| CATGTTCAGAATCTCGAAAA+GGG | - | chr4.2:69567726-69567745 | MS.gene72479:CDS | 35.0% | |
| CTAAAGTAGAATTTGGGAAG+AGG | - | chr4.2:69569166-69569185 | MS.gene72479:intron | 35.0% | |
| CTCAAACATTACACTAACAC+GGG | + | chr4.2:69568714-69568733 | None:intergenic | 35.0% | |
| GACAATCTGTTTCATGGTTA+AGG | - | chr4.2:69569537-69569556 | MS.gene72479:intron | 35.0% | |
| TGGTTAAGGTACAAACACAT+GGG | - | chr4.2:69569551-69569570 | MS.gene72479:intron | 35.0% | |
| TGTTCTAACACATGAAATCG+AGG | + | chr4.2:69568123-69568142 | None:intergenic | 35.0% | |
| TTACGATCATAGTGGTCAAT+GGG | - | chr4.2:69568225-69568244 | MS.gene72479:intron | 35.0% | |
| ! | TATTGTAGTTTGCAGTGACT+TGG | - | chr4.2:69567832-69567851 | MS.gene72479:intron | 35.0% |
| !! | GTAATGTGAAGCACTTTTGT+AGG | + | chr4.2:69567908-69567927 | None:intergenic | 35.0% |
| !! | TAGAAAGGGAGTGTTTCAAA+TGG | + | chr4.2:69569312-69569331 | None:intergenic | 35.0% |
| AAGTGCTTCACATTACCACT+AGG | - | chr4.2:69567912-69567931 | MS.gene72479:intron | 40.0% | |
| ACATCGATCACAATTGAACC+CGG | - | chr4.2:69569645-69569664 | MS.gene72479:CDS | 40.0% | |
| ACTGTCCAATCTGAATCCAA+CGG | - | chr4.2:69568314-69568333 | MS.gene72479:intron | 40.0% | |
| CATTTACGCAATCAACGCTT+TGG | + | chr4.2:69568343-69568362 | None:intergenic | 40.0% | |
| GAAAATAGTTGCGGGCATTT+GGG | + | chr4.2:69569460-69569479 | None:intergenic | 40.0% | |
| GATAAGCATCTTAGGGTGAA+GGG | - | chr4.2:69567869-69567888 | MS.gene72479:intron | 40.0% | |
| GTAATCTGCTTGACAACATC+AGG | + | chr4.2:69569627-69569646 | None:intergenic | 40.0% | |
| GTTACGATCATAGTGGTCAA+TGG | - | chr4.2:69568224-69568243 | MS.gene72479:intron | 40.0% | |
| TCAGCTCCATGAACTAATTC+GGG | - | chr4.2:69569400-69569419 | MS.gene72479:CDS | 40.0% | |
| TGAAAATAGTTGCGGGCATT+TGG | + | chr4.2:69569461-69569480 | None:intergenic | 40.0% | |
| TGCCAAGGATAAGCATCTTA+GGG | - | chr4.2:69567862-69567881 | MS.gene72479:intron | 40.0% | |
| ! | AGAATTTGGGAAGAGGGTAT+TGG | - | chr4.2:69569173-69569192 | MS.gene72479:intron | 40.0% |
| ! | GTTTTGTCAGACACAAGTGT+CGG | + | chr4.2:69568176-69568195 | None:intergenic | 40.0% |
| ! | TGAGATACTGTGGTTGCTTT+CGG | - | chr4.2:69568583-69568602 | MS.gene72479:intron | 40.0% |
| ! | TTTTGTCAGACACAAGTGTC+GGG | + | chr4.2:69568175-69568194 | None:intergenic | 40.0% |
| !! | GAATTTGGGAAGAGGGTATT+GGG | - | chr4.2:69569174-69569193 | MS.gene72479:intron | 40.0% |
| !! | GTGGTTCTAGAGATGTACTA+GGG | - | chr4.2:69568606-69568625 | MS.gene72479:intron | 40.0% |
| ACCACTAGGAAAACTCCTTG+TGG | - | chr4.2:69567926-69567945 | MS.gene72479:intron | 45.0% | |
| AGGAAAACTCCTTGTGGAGA+AGG | - | chr4.2:69567932-69567951 | MS.gene72479:intron | 45.0% | |
| CAACATCAGGTGAACTGTAG+AGG | + | chr4.2:69569614-69569633 | None:intergenic | 45.0% | |
| CACCCTAAGATGCTTATCCT+TGG | + | chr4.2:69567867-69567886 | None:intergenic | 45.0% | |
| CACTAAGGGTCTGTTTGGAT+TGG | + | chr4.2:69569053-69569072 | None:intergenic | 45.0% | |
| CCAGCAACCAAAATGCACAA+AGG | + | chr4.2:69567987-69568006 | None:intergenic | 45.0% | |
| CTGTCCAATCTGAATCCAAC+GGG | - | chr4.2:69568315-69568334 | MS.gene72479:intron | 45.0% | |
| GAAACCAACTAAGCCTGGTT+TGG | - | chr4.2:69567653-69567672 | MS.gene72479:CDS | 45.0% | |
| GCAATGAAACCAACTAAGCC+TGG | - | chr4.2:69567648-69567667 | MS.gene72479:CDS | 45.0% | |
| GGATAAGCATCTTAGGGTGA+AGG | - | chr4.2:69567868-69567887 | MS.gene72479:intron | 45.0% | |
| GTGCCAAGGATAAGCATCTT+AGG | - | chr4.2:69567861-69567880 | MS.gene72479:intron | 45.0% | |
| TCCTGAGATTCTTCCAAACC+AGG | + | chr4.2:69567669-69567688 | None:intergenic | 45.0% | |
| TCTTCCAAACCAGGCTTAGT+TGG | + | chr4.2:69567660-69567679 | None:intergenic | 45.0% | |
| ! | ACTTTTGTAGGCATCCTCAC+TGG | + | chr4.2:69567896-69567915 | None:intergenic | 45.0% |
| ! | ATCTAAGATGGAGAGTCGCA+AGG | + | chr4.2:69568525-69568544 | None:intergenic | 45.0% |
| ! | GAGATACTGTGGTTGCTTTC+GGG | - | chr4.2:69568584-69568603 | MS.gene72479:intron | 45.0% |
| ! | TCCACAAGGAGTTTTCCTAG+TGG | + | chr4.2:69567930-69567949 | None:intergenic | 45.0% |
| !! | GGTGGTTCTAGAGATGTACT+AGG | - | chr4.2:69568605-69568624 | MS.gene72479:intron | 45.0% |
| !!! | CCTTTGTGCATTTTGGTTGC+TGG | - | chr4.2:69567984-69568003 | MS.gene72479:intron | 45.0% |
| ATCAACGCTTTGGTACCCGT+TGG | + | chr4.2:69568333-69568352 | None:intergenic | 50.0% | |
| GGTAACCTCAACTTCGACAC+CGG | + | chr4.2:69569667-69569686 | None:intergenic | 50.0% | |
| GGTACCCGTTGGATTCAGAT+TGG | + | chr4.2:69568322-69568341 | None:intergenic | 50.0% | |
| GTAACCTCAACTTCGACACC+GGG | + | chr4.2:69569666-69569685 | None:intergenic | 50.0% | |
| ! | ATACTGTGGTTGCTTTCGGG+TGG | - | chr4.2:69568587-69568606 | MS.gene72479:intron | 50.0% |
| !! | GCCTGGTTTGGAAGAATCTC+AGG | - | chr4.2:69567665-69567684 | MS.gene72479:CDS | 50.0% |
| !! | GTTTGCAGTGACTTGGTTCG+TGG | - | chr4.2:69567839-69567858 | MS.gene72479:intron | 50.0% |
| TTAGGGTGAAGGGACCAGTG+AGG | - | chr4.2:69567879-69567898 | MS.gene72479:intron | 55.0% | |
| ! | TGAACCCGGTGTCGAAGTTG+AGG | - | chr4.2:69569659-69569678 | MS.gene72479:CDS | 55.0% |
| !! | TGACTTGGTTCGTGGTGCCA+AGG | - | chr4.2:69567847-69567866 | MS.gene72479:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 69567636 | 69569701 | 69567636 | ID=MS.gene72479 |
| chr4.2 | mRNA | 69567636 | 69569701 | 69567636 | ID=MS.gene72479.t1;Parent=MS.gene72479 |
| chr4.2 | exon | 69569590 | 69569701 | 69569590 | ID=MS.gene72479.t1.exon1;Parent=MS.gene72479.t1 |
| chr4.2 | CDS | 69569590 | 69569701 | 69569590 | ID=cds.MS.gene72479.t1;Parent=MS.gene72479.t1 |
| chr4.2 | exon | 69569384 | 69569497 | 69569384 | ID=MS.gene72479.t1.exon2;Parent=MS.gene72479.t1 |
| chr4.2 | CDS | 69569384 | 69569497 | 69569384 | ID=cds.MS.gene72479.t1;Parent=MS.gene72479.t1 |
| chr4.2 | exon | 69567636 | 69567778 | 69567636 | ID=MS.gene72479.t1.exon3;Parent=MS.gene72479.t1 |
| chr4.2 | CDS | 69567636 | 69567778 | 69567636 | ID=cds.MS.gene72479.t1;Parent=MS.gene72479.t1 |
| Gene Sequence |
| Protein sequence |
