AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072375


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072375.t1 MTR_7g092720 100.000 122 0 0 1 122 1 122 1.11e-87 250
MS.gene072375.t1 MTR_0157s0070 98.361 122 2 0 1 122 1 122 7.09e-87 248
MS.gene072375.t1 MTR_4g034030 98.361 122 2 0 1 122 1 122 7.09e-87 248
MS.gene072375.t1 MTR_2g085090 97.500 120 3 0 3 122 4 123 9.83e-84 240
MS.gene072375.t1 MTR_1g091057 97.500 120 3 0 3 122 4 123 9.83e-84 240
MS.gene072375.t1 MTR_4g034030 98.824 85 1 0 38 122 3 87 3.02e-58 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072375.t1 AT3G47370 89.344 122 13 0 1 122 1 122 2.23e-76 222
MS.gene072375.t1 AT3G47370 89.344 122 13 0 1 122 1 122 2.23e-76 222
MS.gene072375.t1 AT3G47370 89.344 122 13 0 1 122 1 122 2.23e-76 222
MS.gene072375.t1 AT5G62300 89.256 121 13 0 2 122 4 124 1.92e-75 219
MS.gene072375.t1 AT5G62300 89.256 121 13 0 2 122 4 124 1.92e-75 219
MS.gene072375.t1 AT3G45030 89.256 121 13 0 2 122 4 124 1.92e-75 219

Find 42 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATTAGTTTGTGCTGATT+TGG 0.351954 7.4:-16083317 MS.gene072375:intron
AAAGGGTGATCCTAATCTTA+TGG 0.356442 7.4:+16083450 None:intergenic
TTCTCCACAAGGAGTCTTCC+TGG 0.359877 7.4:+16083219 None:intergenic
GTAATCTGTTTAACAACATC+TGG 0.381438 7.4:+16082202 None:intergenic
AATCTTATGGATCTGCTCTT+GGG 0.403761 7.4:+16083463 None:intergenic
TAATCTTATGGATCTGCTCT+TGG 0.405883 7.4:+16083462 None:intergenic
GGATTCCTCCAAACCAGGCT+TGG 0.415376 7.4:+16083484 None:intergenic
GAAACCCACCAAGCCTGGTT+TGG 0.420702 7.4:-16083492 MS.gene072375:CDS
TCCTCCAAACCAGGCTTGGT+GGG 0.429749 7.4:+16083488 None:intergenic
TTCCTCCAAACCAGGCTTGG+TGG 0.433644 7.4:+16083487 None:intergenic
GTGATATGAAGAACCTTAGT+AGG 0.436083 7.4:+16083244 None:intergenic
TTAGAGTGAAGGGTCCTGTT+AGG 0.436691 7.4:-16083270 MS.gene072375:CDS
ACCTTAGTAGGCATCCTAAC+AGG 0.444549 7.4:+16083256 None:intergenic
AAGAGCAGATCCATAAGATT+AGG 0.460004 7.4:-16083460 MS.gene072375:CDS
TTGTTACAGGTACCAACACC+TGG 0.473935 7.4:-16082276 MS.gene072375:intron
TTTGACATGCTTGGAGGAAA+GGG 0.476236 7.4:+16083433 None:intergenic
ACCAGGTTCGATGGTGATAG+AGG 0.489959 7.4:+16082180 None:intergenic
GTTTGTGCTGATTTGGTTCG+TGG 0.496204 7.4:-16083310 MS.gene072375:CDS
GCGATGAAACCCACCAAGCC+TGG 0.496657 7.4:-16083497 MS.gene072375:CDS
CGAACCTGGTGTCGAGGTTG+AGG 0.501443 7.4:-16082167 MS.gene072375:CDS
AGGAAGACTCCTTGTGGAGA+AGG 0.508893 7.4:-16083217 MS.gene072375:intron
TCCTGTTAGGATGCCTACTA+AGG 0.508963 7.4:-16083257 MS.gene072375:CDS
ACCCACCAAGCCTGGTTTGG+AGG 0.512859 7.4:-16083489 MS.gene072375:CDS
TCTTGGGATTCCTCCAAACC+AGG 0.514536 7.4:+16083479 None:intergenic
ACCTCTATCACCATCGAACC+TGG 0.519945 7.4:-16082181 MS.gene072375:CDS
TAATCTGTTTAACAACATCT+GGG 0.529679 7.4:+16082203 None:intergenic
TGATTTGGTTCGTGGCGCTA+AGG 0.536928 7.4:-16083302 MS.gene072375:CDS
AACCTCGACACCAGGTTCGA+TGG 0.552766 7.4:+16082171 None:intergenic
CACCATCGAACCTGGTGTCG+AGG 0.559437 7.4:-16082173 MS.gene072375:CDS
CGATAACTCTCTTGTGCACA+CGG 0.563868 7.4:+16082239 None:intergenic
GGATAAGCATCTTAGAGTGA+AGG 0.564807 7.4:-16083281 MS.gene072375:CDS
TTCAAATCTATCCCAGGTGT+TGG 0.568966 7.4:+16082264 None:intergenic
GATAAGCATCTTAGAGTGAA+GGG 0.578400 7.4:-16083280 MS.gene072375:CDS
GTAACCTCAACCTCGACACC+AGG 0.583580 7.4:+16082163 None:intergenic
CAACATCTGGGGAACTGTAG+AGG 0.590461 7.4:+16082215 None:intergenic
ACGGAGTTCAAATCTATCCC+AGG 0.607565 7.4:+16082258 None:intergenic
AGGTTCTTCATATCACTACC+AGG 0.631821 7.4:-16083237 MS.gene072375:CDS
TTAAGATCAAGCATCTGCAA+TGG 0.639875 7.4:+16082141 None:intergenic
AATGAAATACCTTCTCCACA+AGG 0.646547 7.4:+16083208 None:intergenic
ACTACCAGGAAGACTCCTTG+TGG 0.667890 7.4:-16083223 MS.gene072375:CDS
TGTTACAGGTACCAACACCT+GGG 0.689167 7.4:-16082275 MS.gene072375:intron
AATCTGTTTAACAACATCTG+GGG 0.693040 7.4:+16082204 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTCTTTAAAATAATAAAT+GGG - chr7.4:16082817-16082836 MS.gene072375:intron 10.0%
!!! TTTTATAATTATTTGATGAA+AGG - chr7.4:16082514-16082533 MS.gene072375:intron 10.0%
!!! GTTCTCTTTAAAATAATAAA+TGG - chr7.4:16082816-16082835 MS.gene072375:intron 15.0%
!!! TTTAATTCAGTTTTGTTGAA+TGG - chr7.4:16083017-16083036 MS.gene072375:intron 20.0%
! AATTATCCTACAACCTTAAT+TGG - chr7.4:16082919-16082938 MS.gene072375:intron 25.0%
! TAATCTGTTTAACAACATCT+GGG + chr7.4:16083456-16083475 None:intergenic 25.0%
!! TGAGAGCATAAAAAACATAA+AGG + chr7.4:16082281-16082300 None:intergenic 25.0%
!! TTTCATTGTGTTCTATCTTT+TGG - chr7.4:16082891-16082910 MS.gene072375:intron 25.0%
!!! GCATTTTGTTATTATGCTAT+TGG - chr7.4:16083195-16083214 MS.gene072375:intron 25.0%
!!! TGTTTCTGAACAAATTTTGA+AGG - chr7.4:16083062-16083081 MS.gene072375:intron 25.0%
AATCTGTTTAACAACATCTG+GGG + chr7.4:16083455-16083474 None:intergenic 30.0%
AGATTAGAAACATCATTGAG+AGG - chr7.4:16082552-16082571 MS.gene072375:intron 30.0%
ATTTGATGAAAGGTAGTCAA+TGG - chr7.4:16082524-16082543 MS.gene072375:intron 30.0%
GATTAGAAACATCATTGAGA+GGG - chr7.4:16082553-16082572 MS.gene072375:intron 30.0%
GTAATCTGTTTAACAACATC+TGG + chr7.4:16083457-16083476 None:intergenic 30.0%
TAAAGAGAACAAACCAAACA+CGG + chr7.4:16082806-16082825 None:intergenic 30.0%
TCCTAATATGGCTTACAAAT+GGG - chr7.4:16082693-16082712 MS.gene072375:intron 30.0%
! CTAGTTGTAGTTTTTGCAAT+TGG - chr7.4:16082850-16082869 MS.gene072375:intron 30.0%
! GTTTTTCTAAGCTCCTAATA+TGG - chr7.4:16082681-16082700 MS.gene072375:intron 30.0%
!! GTTATTAGTTTGTGCTGATT+TGG - chr7.4:16082339-16082358 MS.gene072375:intron 30.0%
AAAGGGTGATCCTAATCTTA+TGG + chr7.4:16082209-16082228 None:intergenic 35.0%
AAATACGATATAGCCGTGTT+TGG - chr7.4:16082790-16082809 MS.gene072375:intron 35.0%
AAGAGCAGATCCATAAGATT+AGG - chr7.4:16082196-16082215 MS.gene072375:CDS 35.0%
AATCTTATGGATCTGCTCTT+GGG + chr7.4:16082196-16082215 None:intergenic 35.0%
AATGAAATACCTTCTCCACA+AGG + chr7.4:16082451-16082470 None:intergenic 35.0%
ACCCATTTGTAAGCCATATT+AGG + chr7.4:16082697-16082716 None:intergenic 35.0%
ATCCTACACTTCAATGACAA+TGG + chr7.4:16083302-16083321 None:intergenic 35.0%
ATGACCTTACTTCTTGTTAC+AGG - chr7.4:16083367-16083386 MS.gene072375:intron 35.0%
CATGTCAAAAATCTCGAGAA+GGG - chr7.4:16082237-16082256 MS.gene072375:CDS 35.0%
CTCCTAATATGGCTTACAAA+TGG - chr7.4:16082692-16082711 MS.gene072375:intron 35.0%
GATAAGCATCTTAGAGTGAA+GGG - chr7.4:16082376-16082395 MS.gene072375:intron 35.0%
GCATCTAAATTTCCATAAGC+AGG + chr7.4:16082746-16082765 None:intergenic 35.0%
GTGATATGAAGAACCTTAGT+AGG + chr7.4:16082415-16082434 None:intergenic 35.0%
TAATCTTATGGATCTGCTCT+TGG + chr7.4:16082197-16082216 None:intergenic 35.0%
TGTGCATAGAAGAGAATCAA+CGG + chr7.4:16083153-16083172 None:intergenic 35.0%
!! AATTTTGAAGGACTCGACTA+CGG - chr7.4:16083074-16083093 MS.gene072375:intron 35.0%
!!! CTTGATAGTGCTATGTTTTC+TGG - chr7.4:16083259-16083278 MS.gene072375:CDS 35.0%
!!! TTTTTGCAATTGGTTTGTGC+TGG - chr7.4:16082860-16082879 MS.gene072375:intron 35.0%
AGCCATTGTCATTGAAGTGT+AGG - chr7.4:16083297-16083316 MS.gene072375:CDS 40.0%
AGGTTCTTCATATCACTACC+AGG - chr7.4:16082419-16082438 MS.gene072375:intron 40.0%
CACTATCAAGTATTCAGCAG+AGG + chr7.4:16083249-16083268 None:intergenic 40.0%
CGTATGCCAATTAAGGTTGT+AGG + chr7.4:16082928-16082947 None:intergenic 40.0%
GCATGTCAAAAATCTCGAGA+AGG - chr7.4:16082236-16082255 MS.gene072375:CDS 40.0%
GGATAAGCATCTTAGAGTGA+AGG - chr7.4:16082375-16082394 MS.gene072375:intron 40.0%
GGTACCTGTAACAAGAAGTA+AGG + chr7.4:16083374-16083393 None:intergenic 40.0%
TACGTGTGAGAGTAAAAGGA+AGG - chr7.4:16082944-16082963 MS.gene072375:intron 40.0%
TCAAGTATTCAGCAGAGGTT+TGG + chr7.4:16083244-16083263 None:intergenic 40.0%
TCTCACACGTATGCCAATTA+AGG + chr7.4:16082935-16082954 None:intergenic 40.0%
TGAGAGGGAACTGTTGAAAT+AGG - chr7.4:16082568-16082587 MS.gene072375:intron 40.0%
TGATTCTCTTCTATGCACAC+TGG - chr7.4:16083154-16083173 MS.gene072375:intron 40.0%
TTCAAATCTATCCCAGGTGT+TGG + chr7.4:16083395-16083414 None:intergenic 40.0%
TTTGACATGCTTGGAGGAAA+GGG + chr7.4:16082226-16082245 None:intergenic 40.0%
! CTCGAGATTTTTGACATGCT+TGG + chr7.4:16082235-16082254 None:intergenic 40.0%
! GAGATTTTTGACATGCTTGG+AGG + chr7.4:16082232-16082251 None:intergenic 40.0%
! TTTTGACATGCTTGGAGGAA+AGG + chr7.4:16082227-16082246 None:intergenic 40.0%
!! ATTTTGAAGGACTCGACTAC+GGG - chr7.4:16083075-16083094 MS.gene072375:intron 40.0%
ACCTTAGTAGGCATCCTAAC+AGG + chr7.4:16082403-16082422 None:intergenic 45.0%
ACGGAGTTCAAATCTATCCC+AGG + chr7.4:16083401-16083420 None:intergenic 45.0%
CGATAACTCTCTTGTGCACA+CGG + chr7.4:16083420-16083439 None:intergenic 45.0%
GGCATACGTGTGAGAGTAAA+AGG - chr7.4:16082940-16082959 MS.gene072375:intron 45.0%
TCCTGTTAGGATGCCTACTA+AGG - chr7.4:16082399-16082418 MS.gene072375:intron 45.0%
TGTTACAGGTACCAACACCT+GGG - chr7.4:16083381-16083400 MS.gene072375:intron 45.0%
TTAGAGTGAAGGGTCCTGTT+AGG - chr7.4:16082386-16082405 MS.gene072375:intron 45.0%
TTGTTACAGGTACCAACACC+TGG - chr7.4:16083380-16083399 MS.gene072375:intron 45.0%
! TTCTATGCACACTGGTTGCA+AGG - chr7.4:16083162-16083181 MS.gene072375:intron 45.0%
!! CCATAAGCAGGCTAGTTTCA+GGG + chr7.4:16082734-16082753 None:intergenic 45.0%
!! CGACTACGGGTTGGAATTAA+TGG - chr7.4:16083088-16083107 MS.gene072375:intron 45.0%
!! GTTTGTGCTGATTTGGTTCG+TGG - chr7.4:16082346-16082365 MS.gene072375:intron 45.0%
!! TCCATAAGCAGGCTAGTTTC+AGG + chr7.4:16082735-16082754 None:intergenic 45.0%
ACCAGGTTCGATGGTGATAG+AGG + chr7.4:16083479-16083498 None:intergenic 50.0%
ACCTCTATCACCATCGAACC+TGG - chr7.4:16083475-16083494 MS.gene072375:CDS 50.0%
ACTACCAGGAAGACTCCTTG+TGG - chr7.4:16082433-16082452 MS.gene072375:intron 50.0%
AGGAAGACTCCTTGTGGAGA+AGG - chr7.4:16082439-16082458 MS.gene072375:intron 50.0%
CAACATCTGGGGAACTGTAG+AGG + chr7.4:16083444-16083463 None:intergenic 50.0%
TCTTGGGATTCCTCCAAACC+AGG + chr7.4:16082180-16082199 None:intergenic 50.0%
TTCTCCACAAGGAGTCTTCC+TGG + chr7.4:16082440-16082459 None:intergenic 50.0%
! CCCTGAAACTAGCCTGCTTA+TGG - chr7.4:16082731-16082750 MS.gene072375:intron 50.0%
!! TGATTTGGTTCGTGGCGCTA+AGG - chr7.4:16082354-16082373 MS.gene072375:intron 50.0%
AACCTCGACACCAGGTTCGA+TGG + chr7.4:16083488-16083507 None:intergenic 55.0%
GAAACCCACCAAGCCTGGTT+TGG - chr7.4:16082164-16082183 MS.gene072375:CDS 55.0%
GGATTCCTCCAAACCAGGCT+TGG + chr7.4:16082175-16082194 None:intergenic 55.0%
GTAACCTCAACCTCGACACC+AGG + chr7.4:16083496-16083515 None:intergenic 55.0%
TGAAGGACTCGACTACGGGT+TGG - chr7.4:16083079-16083098 MS.gene072375:intron 55.0%
TTCCTCCAAACCAGGCTTGG+TGG + chr7.4:16082172-16082191 None:intergenic 55.0%
! TCCTCCAAACCAGGCTTGGT+GGG + chr7.4:16082171-16082190 None:intergenic 55.0%
GCGATGAAACCCACCAAGCC+TGG - chr7.4:16082159-16082178 MS.gene072375:CDS 60.0%
! CACCATCGAACCTGGTGTCG+AGG - chr7.4:16083483-16083502 MS.gene072375:CDS 60.0%
! CGAACCTGGTGTCGAGGTTG+AGG - chr7.4:16083489-16083508 MS.gene072375:CDS 60.0%
!! ACCCACCAAGCCTGGTTTGG+AGG - chr7.4:16082167-16082186 MS.gene072375:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 16082147 16083531 16082147 ID=MS.gene072375
chr7.4 mRNA 16082147 16083531 16082147 ID=MS.gene072375.t1;Parent=MS.gene072375
chr7.4 exon 16083420 16083531 16083420 ID=MS.gene072375.t1.exon1;Parent=MS.gene072375.t1
chr7.4 CDS 16083420 16083531 16083420 ID=cds.MS.gene072375.t1;Parent=MS.gene072375.t1
chr7.4 exon 16083218 16083331 16083218 ID=MS.gene072375.t1.exon2;Parent=MS.gene072375.t1
chr7.4 CDS 16083218 16083331 16083218 ID=cds.MS.gene072375.t1;Parent=MS.gene072375.t1
chr7.4 exon 16082147 16082289 16082147 ID=MS.gene072375.t1.exon3;Parent=MS.gene072375.t1
chr7.4 CDS 16082147 16082289 16082147 ID=cds.MS.gene072375.t1;Parent=MS.gene072375.t1
Gene Sequence

>MS.gene072375

ATGGCGTACGCTGCGATGAAACCCACCAAGCCTGGTTTGGAGGAATCCCAAGAGCAGATCCATAAGATTAGGATCACCCTTTCCTCCAAGCATGTCAAAAATCTCGAGAAGGGTACGTTCTGTTAATGTTTCCTTTATGTTTTTTATGCTCTCAGTACGACGCTGACATTTCAACTTTCACTTGTTATTGTTGTTATTAGTTTGTGCTGATTTGGTTCGTGGCGCTAAGGATAAGCATCTTAGAGTGAAGGGTCCTGTTAGGATGCCTACTAAGGTTCTTCATATCACTACCAGGAAGACTCCTTGTGGAGAAGGTATTTCATTTTATCTATGTTTTTATTTTCACTTTATTTCATACTAATAGAGATTTTATAATTATTTGATGAAAGGTAGTCAATGGCTAATAGATTAGAAACATCATTGAGAGGGAACTGTTGAAATAGGTTATTTAGAGAGTATTTGTATTATTTTATAGCATAAGAATTTATCAAAGCGTTACATTTATTGATATATAAGCTGTTGAAGCTTACTTATGTTTTTCTAAGCTCCTAATATGGCTTACAAATGGGTGCTTAAGAAATGTGCCCTGAAACTAGCCTGCTTATGGAAATTTAGATGCAAATTGTTGTTGTAATTGTAATTTAAATACGATATAGCCGTGTTTGGTTTGTTCTCTTTAAAATAATAAATGGGCAACTTTTGACTAGTTGTAGTTTTTGCAATTGGTTTGTGCTGGTTTGTCAGTTTCATTGTGTTCTATCTTTTGGATGTCAATTATCCTACAACCTTAATTGGCATACGTGTGAGAGTAAAAGGAAGGTTTCTGTTTTCTAACTTTGTTTTTATAAGACAGCAACTTGTCTAAATCTTTTTAATTCAGTTTTGTTGAATGGTTTACGATAACTTGCATCAGAATGTTTCTGAACAAATTTTGAAGGACTCGACTACGGGTTGGAATTAATGGATCTTTAAATGTTTTACATATAGTTATTTATTAATCTCACCGTTGATTCTCTTCTATGCACACTGGTTGCAAGGTTTCTCTTTTGCATTTTGTTATTATGCTATTGGTTTTATGTGTGTATTTGTGTATGCCAAACCTCTGCTGAATACTTGATAGTGCTATGTTTTCTGGTTTTATCTCAAGTCAAGCCATTGTCATTGAAGTGTAGGATTCTCTGAATTTTTTTGCTGAATGAATGATATTCTGTCTTGTGTTCATGACCTTACTTCTTGTTACAGGTACCAACACCTGGGATAGATTTGAACTCCGTGTGCACAAGAGAGTTATCGACCTCTACAGTTCCCCAGATGTTGTTAAACAGATTACCTCTATCACCATCGAACCTGGTGTCGAGGTTGAGGTTACCATTGCAGATGCTTGA

Protein sequence

>MS.gene072375.t1

MAYAAMKPTKPGLEESQEQIHKIRITLSSKHVKNLEKVCADLVRGAKDKHLRVKGPVRMPTKVLHITTRKTPCGEGTNTWDRFELRVHKRVIDLYSSPDVVKQITSITIEPGVEVEVTIADA