AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02029

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02029.t1 MTR_2g028870 96.742 399 11 2 2 399 4 401 0.0 781
MS.gene02029.t1 MTR_2g044290 40.526 190 102 3 211 399 80 259 6.71e-41 145
MS.gene02029.t1 MTR_2g044420 40.526 190 102 3 211 399 87 266 1.54e-39 142
MS.gene02029.t1 MTR_2g044440 37.368 190 108 3 211 399 96 275 5.54e-38 139
MS.gene02029.t1 MTR_2g044250 38.947 190 105 2 211 399 69 248 1.86e-37 136
MS.gene02029.t1 MTR_4g063810 39.572 187 102 3 211 396 91 267 1.88e-36 135
MS.gene02029.t1 MTR_4g063815 36.757 185 106 2 216 399 60 234 1.51e-35 131
MS.gene02029.t1 MTR_2g044240 35.294 221 131 4 180 399 37 246 1.98e-35 131
MS.gene02029.t1 MTR_3g462500 34.978 223 133 5 178 397 29 242 9.88e-33 124
MS.gene02029.t1 MTR_8g022900 31.939 263 150 8 140 398 4 241 9.36e-32 121
MS.gene02029.t1 MTR_1g105460 34.759 187 109 2 218 399 106 284 1.27e-31 122
MS.gene02029.t1 MTR_0777s0010 31.818 242 147 6 161 399 203 429 1.43e-30 122
MS.gene02029.t1 MTR_3g462590 32.642 193 114 4 211 399 97 277 9.40e-29 114
MS.gene02029.t1 MTR_3g462570 36.126 191 111 4 211 399 46 227 2.76e-28 117
MS.gene02029.t1 MTR_3g462570 37.398 123 60 4 281 399 415 524 2.33e-16 81.3
MS.gene02029.t1 MTR_1g105450 40.310 129 69 1 271 399 3 123 9.99e-28 107
MS.gene02029.t1 MTR_5g062990 33.333 195 114 4 210 399 104 287 7.13e-27 109
MS.gene02029.t1 MTR_3g463280 33.526 173 109 1 227 399 49 215 1.07e-26 107
MS.gene02029.t1 MTR_3g462560 35.106 188 109 5 211 397 53 228 1.85e-26 107
MS.gene02029.t1 MTR_1g105465 32.804 189 105 4 211 397 146 314 6.90e-26 107
MS.gene02029.t1 MTR_3g462530 37.748 151 84 3 250 399 42 183 4.84e-25 104
MS.gene02029.t1 MTR_3g463310 30.151 199 124 2 209 399 24 215 5.74e-24 99.8
MS.gene02029.t1 MTR_2g044260 30.348 201 118 4 211 399 81 271 2.12e-21 93.6
MS.gene02029.t1 MTR_5g078410 32.984 191 96 7 211 399 157 317 2.96e-21 94.4
MS.gene02029.t1 MTR_3g462430 40.650 123 58 4 281 399 117 228 3.01e-20 89.7
MS.gene02029.t1 MTR_2g038640 29.255 188 87 5 211 397 157 299 2.69e-19 88.6
MS.gene02029.t1 MTR_3g462490 29.605 152 84 4 229 370 284 422 4.93e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02029.t1 AT4G19670 54.177 395 152 6 17 399 20 397 2.78e-137 403
MS.gene02029.t1 AT4G19670 54.177 395 152 6 17 399 20 397 2.78e-137 403
MS.gene02029.t1 AT4G19670 54.177 395 152 6 17 399 20 397 2.78e-137 403
MS.gene02029.t1 AT4G19670 54.177 395 152 6 17 399 20 397 2.78e-137 403
MS.gene02029.t1 AT4G19670 54.177 395 152 6 17 399 20 397 2.78e-137 403
MS.gene02029.t1 AT4G19670 54.177 395 152 6 17 399 20 397 2.78e-137 403
MS.gene02029.t1 AT4G19670 54.177 395 152 6 17 399 20 397 2.78e-137 403
MS.gene02029.t1 AT3G53690 39.267 191 106 3 210 399 114 295 7.13e-38 139
MS.gene02029.t1 AT5G60250 30.372 349 224 9 65 399 146 489 9.50e-38 144
MS.gene02029.t1 AT2G25370 29.598 348 227 10 59 399 149 485 1.88e-36 140
MS.gene02029.t1 AT2G25360 31.741 293 169 10 117 399 1 272 1.57e-35 134
MS.gene02029.t1 AT5G37560 27.536 345 233 8 59 399 31 362 1.19e-34 133
MS.gene02029.t1 AT3G45580 27.717 368 247 8 38 399 2 356 8.67e-34 130
MS.gene02029.t1 AT3G14250 31.959 194 119 3 211 399 95 280 1.80e-31 122
MS.gene02029.t1 AT2G25370 29.023 348 202 13 59 399 119 428 3.96e-29 119
MS.gene02029.t1 AT3G45470 35.751 193 112 6 209 399 4 186 5.48e-29 113
MS.gene02029.t1 AT2G21420 25.434 346 228 9 60 399 46 367 2.25e-24 104
MS.gene02029.t1 AT3G43750 25.648 347 225 13 65 399 14 339 2.38e-23 100
MS.gene02029.t1 AT3G45555 32.812 192 119 4 209 398 15 198 6.85e-23 96.3
MS.gene02029.t1 AT2G26135 32.642 193 118 6 209 399 151 333 1.53e-22 98.6
MS.gene02029.t1 AT2G19610 24.217 351 243 11 63 399 49 390 1.59e-21 95.9
MS.gene02029.t1 AT2G19610 24.207 347 248 10 63 399 49 390 2.07e-21 95.9
MS.gene02029.t1 AT3G45540 33.846 195 114 6 210 399 99 283 3.17e-21 94.4
MS.gene02029.t1 AT3G45540 33.846 195 114 6 210 399 70 254 6.12e-21 93.2
MS.gene02029.t1 AT2G26130 26.023 342 230 11 65 399 15 340 1.44e-20 93.2
MS.gene02029.t1 AT3G45480 26.590 346 204 13 66 399 21 328 1.92e-20 92.4
MS.gene02029.t1 AT3G45570 24.595 309 199 9 63 356 15 304 8.55e-16 78.2
MS.gene02029.t1 AT3G45460 23.032 343 228 7 63 399 28 340 4.54e-14 73.6
MS.gene02029.t1 AT3G45510 29.268 164 105 4 193 354 50 204 2.50e-13 70.1

Find 80 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGTAAGAATGTTCTTTA+AGG 0.119190 2.2:+58913577 MS.gene02029:CDS
ATCAAAAGTTTCAAAATTAT+TGG 0.141167 2.2:-58914733 None:intergenic
TTCTGGAATTGGAGTGTTTA+TGG 0.181374 2.2:+58913640 MS.gene02029:CDS
CATCAAATCTTCCTCCTATA+AGG 0.240562 2.2:+58913669 MS.gene02029:CDS
TTTAAGGGGTTATCAATTAC+TGG 0.264313 2.2:+58913593 MS.gene02029:CDS
CCTGGTTGAGGACACCTTAT+AGG 0.268746 2.2:-58914981 None:intergenic
TGATTCTGAGTTGTTATATA+AGG 0.290859 2.2:+58913805 MS.gene02029:CDS
GGTGTTGAAAATTCAACTTC+AGG 0.327371 2.2:+58913614 MS.gene02029:CDS
GGCACTGAGAGAGAGGATTC+TGG 0.344286 2.2:+58914704 MS.gene02029:CDS
TCAGTAAGAATGTTCTTTAA+GGG 0.369895 2.2:+58913578 MS.gene02029:CDS
GAAGCATCTCTCTCCTCTTC+CGG 0.382920 2.2:-58915299 None:intergenic
CTCCTATAAGGGTGCAGAAA+AGG 0.394666 2.2:+58913681 MS.gene02029:CDS
GAAAGTGGTGGAAGGGTTGA+AGG 0.395192 2.2:+58913538 MS.gene02029:CDS
CATTTCAAGGTAATCATCAT+TGG 0.403717 2.2:-58914891 None:intergenic
CTGAAATTCTTCACAGCTCA+TGG 0.404489 2.2:-58915270 None:intergenic
CAGATAAACCTCTGACAAAC+AGG 0.405918 2.2:-58915218 None:intergenic
GGAGCCTATGGTTGATTACT+TGG 0.414618 2.2:+58913721 MS.gene02029:CDS
ATCGTCTTGCTCAGAATAAA+AGG 0.418491 2.2:+58915336 MS.gene02029:CDS
GACAATATTTGCTTCTGAAA+GGG 0.428917 2.2:-58915078 None:intergenic
CTGGTTGAGGACACCTTATA+GGG 0.432518 2.2:-58914980 None:intergenic
AGAACAGAACAATTCAGAAA+TGG 0.439725 2.2:-58915122 None:intergenic
GGTTGATTACTTGGCACTCA+TGG 0.440139 2.2:+58913730 MS.gene02029:CDS
GATTACTTGGCACTCATGGA+TGG 0.444840 2.2:+58913734 MS.gene02029:CDS
ATCAAATCTTCCTCCTATAA+GGG 0.451784 2.2:+58913670 MS.gene02029:CDS
GTGTTGAAAATTCAACTTCA+GGG 0.453071 2.2:+58913615 MS.gene02029:CDS
AATGTTGTCAAGTCCCTATA+AGG 0.455085 2.2:+58914967 MS.gene02029:CDS
GCGTGGGCTGTGGTGTTCCT+TGG 0.456811 2.2:+58915240 MS.gene02029:CDS
GAATGTGTGTGAACATTTCA+AGG 0.459619 2.2:-58914904 None:intergenic
ATTTCAGAGTTTGCCGGAAG+AGG 0.462977 2.2:+58915286 MS.gene02029:CDS
GCAGTTGGCCACAGTAGCTA+TGG 0.467745 2.2:+58914794 MS.gene02029:CDS
TCCTCCTCAGCATTAACAAA+TGG 0.468319 2.2:-58913416 None:intergenic
GACAATAAAATCTTTCTGAC+TGG 0.469920 2.2:-58915100 None:intergenic
GAGAAGAAGAACAAGAAGAG+TGG 0.486779 2.2:+58913501 MS.gene02029:CDS
TGAAGAATTTCAGAGTTTGC+CGG 0.489641 2.2:+58915280 MS.gene02029:CDS
AAGGTAATCATCATTGGCAC+TGG 0.492248 2.2:-58914885 None:intergenic
CAGTGAAAGCATAAACCTTA+CGG 0.493680 2.2:-58913784 None:intergenic
GAAAGAAACTGAGGAGAAAG+TGG 0.499489 2.2:+58913523 MS.gene02029:CDS
GGACAATATTTGCTTCTGAA+AGG 0.503697 2.2:-58915079 None:intergenic
TTGCGAGTGTTTATCTGCAA+TGG 0.505329 2.2:+58915154 MS.gene02029:CDS
ATTTGTTAATGCTGAGGAGG+AGG 0.508035 2.2:+58913418 MS.gene02029:CDS
CAATATCTGCATCCTGGTTG+AGG 0.511617 2.2:-58914993 None:intergenic
CATTCTGTTCACATTGCTTG+AGG 0.513889 2.2:+58914922 MS.gene02029:CDS
AGAGATTCGAAGGAGATGAA+TGG 0.514554 2.2:-58915047 None:intergenic
ATAAACACTCGCAAGGATCA+AGG 0.518216 2.2:-58915145 None:intergenic
ATTACTTGGCACTCATGGAT+GGG 0.518596 2.2:+58913735 MS.gene02029:CDS
CTGAGGAGAAAGTGGTGGAA+GGG 0.519617 2.2:+58913531 MS.gene02029:CDS
GCTACTGTGGCCAACTGCAT+TGG 0.532113 2.2:-58914789 None:intergenic
GGACGGTTCATCTAGTCAAT+CGG 0.533188 2.2:+58915175 MS.gene02029:CDS
ACTGAGGAGAAAGTGGTGGA+AGG 0.534801 2.2:+58913530 MS.gene02029:CDS
AGGTTTATCTGCGTGGGCTG+TGG 0.537486 2.2:+58915230 MS.gene02029:CDS
CAGTAAGAATGTTCTTTAAG+GGG 0.542297 2.2:+58913579 MS.gene02029:CDS
GGCTGCTCAAGATCAATGCT+TGG 0.543956 2.2:-58914768 None:intergenic
GGCTTTCTCGAGAGATTCGA+AGG 0.544683 2.2:-58915057 None:intergenic
CCTATAAGGTGTCCTCAACC+AGG 0.547002 2.2:+58914981 MS.gene02029:CDS
ATTCTGAGCAAGACGATGCA+AGG 0.547923 2.2:-58915330 None:intergenic
GAGATACAATATCTGCATCC+TGG 0.555980 2.2:-58914999 None:intergenic
TGTCAGAGGTTTATCTGCGT+GGG 0.560451 2.2:+58915224 MS.gene02029:CDS
TCTTGAGCAGCCAATGCAGT+TGG 0.570997 2.2:+58914779 MS.gene02029:CDS
TGAACCATTTGTTAATGCTG+AGG 0.571520 2.2:+58913412 MS.gene02029:CDS
ATAATGATTGAGCTTACTCA+AGG 0.572588 2.2:+58915383 MS.gene02029:CDS
TCGAGTGTCCTGTTTGTCAG+AGG 0.573238 2.2:+58915210 MS.gene02029:CDS
TTGTAGTTTACCATCAGCAT+AGG 0.582700 2.2:-58914946 None:intergenic
TTGTCAGAGGTTTATCTGCG+TGG 0.583179 2.2:+58915223 MS.gene02029:CDS
AGAAACTGAGGAGAAAGTGG+TGG 0.587296 2.2:+58913526 MS.gene02029:CDS
ACCATTTGTTAATGCTGAGG+AGG 0.591850 2.2:+58913415 MS.gene02029:CDS
CAGTTGGCCACAGTAGCTAT+GGG 0.598216 2.2:+58914795 MS.gene02029:CDS
GTTGTTACCACATGACATGC+CGG 0.606421 2.2:+58915405 MS.gene02029:CDS
TGGAAGAAAAGATTTGCACT+CGG 0.609713 2.2:-58915027 None:intergenic
AGAAGAGTGGAAAGAAACTG+AGG 0.609832 2.2:+58913514 MS.gene02029:CDS
GGAAGAAAAGATTTGCACTC+GGG 0.610101 2.2:-58915026 None:intergenic
TTTGCAGAACAAGATCCGTA+AGG 0.611189 2.2:+58913769 MS.gene02029:CDS
GAGTGTTTATCTGCAATGGA+CGG 0.611968 2.2:+58915158 MS.gene02029:CDS
AGTGCCAAGTAATCAACCAT+AGG 0.620002 2.2:-58913725 None:intergenic
TCTCTCAGTGCCATCAAAAG+CGG 0.620482 2.2:-58914693 None:intergenic
TGCTTGAGGTCCTATGCTGA+TGG 0.631116 2.2:+58914936 MS.gene02029:CDS
TGTCTTACCGGCATGTCATG+TGG 0.634948 2.2:-58915412 None:intergenic
ATTGCAGATAAACACTCGCA+AGG 0.647207 2.2:-58915152 None:intergenic
GACGAGTCCCATAGCTACTG+TGG 0.655389 2.2:-58914802 None:intergenic
CAGCTCATGGAAGAATGCCA+AGG 0.660014 2.2:-58915257 None:intergenic
GTCTTGCTCAGAATAAAAGG+TGG 0.671451 2.2:+58915339 MS.gene02029:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCAATTTAACAATTAT+AGG - chr2.2:58913916-58913935 None:intergenic 10.0%
!!! TTTTCAATTTAACAATTATA+GGG - chr2.2:58913915-58913934 None:intergenic 10.0%
!! ATCAAAAGTTTCAAAATTAT+TGG - chr2.2:58914736-58914755 None:intergenic 15.0%
!!! AGTTTGTTTATTACATGTTT+GGG - chr2.2:58914189-58914208 None:intergenic 20.0%
!!! ATTGTAATTTGTATCTTGAA+TGG + chr2.2:58914427-58914446 MS.gene02029:intron 20.0%
!!! GCATATGATATTATTTTAAG+AGG + chr2.2:58914525-58914544 MS.gene02029:intron 20.0%
! AACAAACATAGTTAAAGACA+AGG - chr2.2:58914624-58914643 None:intergenic 25.0%
! ACAAACATAGTTAAAGACAA+GGG - chr2.2:58914623-58914642 None:intergenic 25.0%
! AGAAAGGAAAATTCAAATCA+AGG - chr2.2:58914480-58914499 None:intergenic 25.0%
! ATGCTAAAGAAACTAAACAA+GGG - chr2.2:58914509-58914528 None:intergenic 25.0%
! TAACTATGTTTGTTTGAATG+TGG + chr2.2:58914630-58914649 MS.gene02029:intron 25.0%
! TATGCTAAAGAAACTAAACA+AGG - chr2.2:58914510-58914529 None:intergenic 25.0%
! TCAGTAAGAATGTTCTTTAA+GGG + chr2.2:58913578-58913597 MS.gene02029:CDS 25.0%
! TGATTCAGTAATTGATCTTA+AGG + chr2.2:58913836-58913855 MS.gene02029:intron 25.0%
! TGATTCTGAGTTGTTATATA+AGG + chr2.2:58913805-58913824 MS.gene02029:CDS 25.0%
! TTCAGTAAGAATGTTCTTTA+AGG + chr2.2:58913577-58913596 MS.gene02029:CDS 25.0%
!! ATTTTTGTCGTCAGTTATTT+AGG + chr2.2:58914261-58914280 MS.gene02029:intron 25.0%
!! GAGTTTGTTTATTACATGTT+TGG - chr2.2:58914190-58914209 None:intergenic 25.0%
!! GATTTGAATTTTCCTTTCTT+TGG + chr2.2:58914481-58914500 MS.gene02029:intron 25.0%
!!! AATATTATTTCTACAGCAAC+TGG + chr2.2:58914041-58914060 MS.gene02029:intron 25.0%
!!! TAAAGTTTTGGTCAATTCTT+GGG + chr2.2:58914355-58914374 MS.gene02029:intron 25.0%
!!! TATTGCTTACTCTAAAGTTT+TGG + chr2.2:58914343-58914362 MS.gene02029:intron 25.0%
ACACTTTAAACAGACATACA+CGG - chr2.2:58914233-58914252 None:intergenic 30.0%
ACAGAGTTAAGAGTTAATAG+AGG - chr2.2:58914582-58914601 None:intergenic 30.0%
AGAACAGAACAATTCAGAAA+TGG - chr2.2:58915125-58915144 None:intergenic 30.0%
ATAATGATTGAGCTTACTCA+AGG + chr2.2:58915383-58915402 MS.gene02029:CDS 30.0%
ATCAAATCTTCCTCCTATAA+GGG + chr2.2:58913670-58913689 MS.gene02029:CDS 30.0%
ATGAAAACAACCTATTACAC+AGG - chr2.2:58914159-58914178 None:intergenic 30.0%
CAGTAAGAATGTTCTTTAAG+GGG + chr2.2:58913579-58913598 MS.gene02029:CDS 30.0%
CATTTCAAGGTAATCATCAT+TGG - chr2.2:58914894-58914913 None:intergenic 30.0%
GACAATAAAATCTTTCTGAC+TGG - chr2.2:58915103-58915122 None:intergenic 30.0%
GATAACAGCTGAGAAAAATT+TGG + chr2.2:58914665-58914684 MS.gene02029:intron 30.0%
GTGTTGAAAATTCAACTTCA+GGG + chr2.2:58913615-58913634 MS.gene02029:CDS 30.0%
TGCTAAAGAAACTAAACAAG+GGG - chr2.2:58914508-58914527 None:intergenic 30.0%
TTGTTGTAAGAGCTTAACTA+AGG - chr2.2:58914000-58914019 None:intergenic 30.0%
TTTAAGGGGTTATCAATTAC+TGG + chr2.2:58913593-58913612 MS.gene02029:CDS 30.0%
! GACAATATTTGCTTCTGAAA+GGG - chr2.2:58915081-58915100 None:intergenic 30.0%
!! CTAAAGTTTTGGTCAATTCT+TGG + chr2.2:58914354-58914373 MS.gene02029:intron 30.0%
!! CTTCTCATGTTTTACTTTTG+TGG + chr2.2:58914288-58914307 MS.gene02029:intron 30.0%
!! GTTGTTTTCATAAGCTATCA+TGG + chr2.2:58914099-58914118 MS.gene02029:intron 30.0%
AATGTTGTCAAGTCCCTATA+AGG + chr2.2:58914967-58914986 MS.gene02029:CDS 35.0%
ATAGAGGTTATGTCAAAGCA+TGG - chr2.2:58914566-58914585 None:intergenic 35.0%
ATCGTCTTGCTCAGAATAAA+AGG + chr2.2:58915336-58915355 MS.gene02029:CDS 35.0%
CATCAAATCTTCCTCCTATA+AGG + chr2.2:58913669-58913688 MS.gene02029:CDS 35.0%
GAATGTGTGTGAACATTTCA+AGG - chr2.2:58914907-58914926 None:intergenic 35.0%
GGACAATATTTGCTTCTGAA+AGG - chr2.2:58915082-58915101 None:intergenic 35.0%
TGAAGAATTTCAGAGTTTGC+CGG + chr2.2:58915280-58915299 MS.gene02029:CDS 35.0%
TGGAAGAAAAGATTTGCACT+CGG - chr2.2:58915030-58915049 None:intergenic 35.0%
TTGTAGTTTACCATCAGCAT+AGG - chr2.2:58914949-58914968 None:intergenic 35.0%
! AAGGCTTGATTTTTATGCTG+AGG + chr2.2:58913700-58913719 MS.gene02029:CDS 35.0%
! AATTCAACTTCAGGGTTTTC+TGG + chr2.2:58913623-58913642 MS.gene02029:CDS 35.0%
! AGCAATTTTTCACCAGTTAC+CGG - chr2.2:58914834-58914853 None:intergenic 35.0%
! CAGTGAAAGCATAAACCTTA+CGG - chr2.2:58913787-58913806 None:intergenic 35.0%
! GGTGTTGAAAATTCAACTTC+AGG + chr2.2:58913614-58913633 MS.gene02029:CDS 35.0%
! TGAACCATTTGTTAATGCTG+AGG + chr2.2:58913412-58913431 MS.gene02029:CDS 35.0%
!! TTCTGGAATTGGAGTGTTTA+TGG + chr2.2:58913640-58913659 MS.gene02029:CDS 35.0%
AACAGACATACACGGTTGTT+TGG - chr2.2:58914225-58914244 None:intergenic 40.0%
AAGGTAATCATCATTGGCAC+TGG - chr2.2:58914888-58914907 None:intergenic 40.0%
AGAAGAGTGGAAAGAAACTG+AGG + chr2.2:58913514-58913533 MS.gene02029:CDS 40.0%
AGAGATTCGAAGGAGATGAA+TGG - chr2.2:58915050-58915069 None:intergenic 40.0%
AGTGCCAAGTAATCAACCAT+AGG - chr2.2:58913728-58913747 None:intergenic 40.0%
ATAAACACTCGCAAGGATCA+AGG - chr2.2:58915148-58915167 None:intergenic 40.0%
ATTACTTGGCACTCATGGAT+GGG + chr2.2:58913735-58913754 MS.gene02029:CDS 40.0%
ATTGCAGATAAACACTCGCA+AGG - chr2.2:58915155-58915174 None:intergenic 40.0%
CAGATAAACCTCTGACAAAC+AGG - chr2.2:58915221-58915240 None:intergenic 40.0%
CATTCTGTTCACATTGCTTG+AGG + chr2.2:58914922-58914941 MS.gene02029:CDS 40.0%
CTGAAATTCTTCACAGCTCA+TGG - chr2.2:58915273-58915292 None:intergenic 40.0%
GAAAGAAACTGAGGAGAAAG+TGG + chr2.2:58913523-58913542 MS.gene02029:CDS 40.0%
GAGAAGAAGAACAAGAAGAG+TGG + chr2.2:58913501-58913520 MS.gene02029:CDS 40.0%
GAGATACAATATCTGCATCC+TGG - chr2.2:58915002-58915021 None:intergenic 40.0%
GAGTGTTTATCTGCAATGGA+CGG + chr2.2:58915158-58915177 MS.gene02029:CDS 40.0%
GGAAGAAAAGATTTGCACTC+GGG - chr2.2:58915029-58915048 None:intergenic 40.0%
GTCTTGCTCAGAATAAAAGG+TGG + chr2.2:58915339-58915358 MS.gene02029:CDS 40.0%
TAAACAAGGGGTCCAAAGAA+AGG - chr2.2:58914496-58914515 None:intergenic 40.0%
TCCTCCTCAGCATTAACAAA+TGG - chr2.2:58913419-58913438 None:intergenic 40.0%
TTGCGAGTGTTTATCTGCAA+TGG + chr2.2:58915154-58915173 MS.gene02029:CDS 40.0%
TTTGCAGAACAAGATCCGTA+AGG + chr2.2:58913769-58913788 MS.gene02029:CDS 40.0%
! ACCATTTGTTAATGCTGAGG+AGG + chr2.2:58913415-58913434 MS.gene02029:CDS 40.0%
! ACTTTTGTGGATGAACTCTG+AGG + chr2.2:58914301-58914320 MS.gene02029:intron 40.0%
! ATTTGTTAATGCTGAGGAGG+AGG + chr2.2:58913418-58913437 MS.gene02029:CDS 40.0%
! TTTTTATGCTGAGGAGCCTA+TGG + chr2.2:58913709-58913728 MS.gene02029:CDS 40.0%
!!! ACTTCAGGGTTTTCTGGAAT+TGG + chr2.2:58913629-58913648 MS.gene02029:CDS 40.0%
AGAAACTGAGGAGAAAGTGG+TGG + chr2.2:58913526-58913545 MS.gene02029:CDS 45.0%
ATTCTGAGCAAGACGATGCA+AGG - chr2.2:58915333-58915352 None:intergenic 45.0%
ATTTCAGAGTTTGCCGGAAG+AGG + chr2.2:58915286-58915305 MS.gene02029:CDS 45.0%
CAATATCTGCATCCTGGTTG+AGG - chr2.2:58914996-58915015 None:intergenic 45.0%
CTGGTTGAGGACACCTTATA+GGG - chr2.2:58914983-58915002 None:intergenic 45.0%
GATTACTTGGCACTCATGGA+TGG + chr2.2:58913734-58913753 MS.gene02029:CDS 45.0%
GGACGGTTCATCTAGTCAAT+CGG + chr2.2:58915175-58915194 MS.gene02029:CDS 45.0%
GGAGCCTATGGTTGATTACT+TGG + chr2.2:58913721-58913740 MS.gene02029:CDS 45.0%
GGTTGATTACTTGGCACTCA+TGG + chr2.2:58913730-58913749 MS.gene02029:CDS 45.0%
GTTGTTACCACATGACATGC+CGG + chr2.2:58915405-58915424 MS.gene02029:CDS 45.0%
TCTCTCAGTGCCATCAAAAG+CGG - chr2.2:58914696-58914715 None:intergenic 45.0%
TGAGCGTATGCCTGTGTAAT+AGG + chr2.2:58914146-58914165 MS.gene02029:intron 45.0%
TGTCAGAGGTTTATCTGCGT+GGG + chr2.2:58915224-58915243 MS.gene02029:CDS 45.0%
TTGTCAGAGGTTTATCTGCG+TGG + chr2.2:58915223-58915242 MS.gene02029:CDS 45.0%
! AGCCTTTTCTGCACCCTTAT+AGG - chr2.2:58913686-58913705 None:intergenic 45.0%
! CTCCTATAAGGGTGCAGAAA+AGG + chr2.2:58913681-58913700 MS.gene02029:CDS 45.0%
! CTTTTCTGCACCCTTATAGG+AGG - chr2.2:58913683-58913702 None:intergenic 45.0%
!! TTTTGATGGCACTGAGAGAG+AGG + chr2.2:58914697-58914716 MS.gene02029:CDS 45.0%
!!! TATGGGACTCGTCACTTTTC+CGG + chr2.2:58914812-58914831 MS.gene02029:CDS 45.0%
!!! TTTGGACATGCCGCTTTTGA+TGG + chr2.2:58914683-58914702 MS.gene02029:CDS 45.0%
CAGCTCATGGAAGAATGCCA+AGG - chr2.2:58915260-58915279 None:intergenic 50.0%
CAGTTGGCCACAGTAGCTAT+GGG + chr2.2:58914795-58914814 MS.gene02029:CDS 50.0%
CCTGGTTGAGGACACCTTAT+AGG - chr2.2:58914984-58915003 None:intergenic 50.0%
GGCTGCTCAAGATCAATGCT+TGG - chr2.2:58914771-58914790 None:intergenic 50.0%
GGCTTTCTCGAGAGATTCGA+AGG - chr2.2:58915060-58915079 None:intergenic 50.0%
GGGCGTGCTTAAACTAGCAA+TGG + chr2.2:58914375-58914394 MS.gene02029:intron 50.0%
TCGAGTGTCCTGTTTGTCAG+AGG + chr2.2:58915210-58915229 MS.gene02029:CDS 50.0%
TCTTGAGCAGCCAATGCAGT+TGG + chr2.2:58914779-58914798 MS.gene02029:CDS 50.0%
TGCTTGAGGTCCTATGCTGA+TGG + chr2.2:58914936-58914955 MS.gene02029:CDS 50.0%
! ACTGAGGAGAAAGTGGTGGA+AGG + chr2.2:58913530-58913549 MS.gene02029:CDS 50.0%
! CCTATAAGGTGTCCTCAACC+AGG + chr2.2:58914981-58915000 MS.gene02029:CDS 50.0%
! CTGAGGAGAAAGTGGTGGAA+GGG + chr2.2:58913531-58913550 MS.gene02029:CDS 50.0%
! GAAAGTGGTGGAAGGGTTGA+AGG + chr2.2:58913538-58913557 MS.gene02029:CDS 50.0%
! GAAGCATCTCTCTCCTCTTC+CGG - chr2.2:58915302-58915321 None:intergenic 50.0%
!!! CTCGTCACTTTTCCGGTAAC+TGG + chr2.2:58914819-58914838 MS.gene02029:CDS 50.0%
GACGAGTCCCATAGCTACTG+TGG - chr2.2:58914805-58914824 None:intergenic 55.0%
GCAGTTGGCCACAGTAGCTA+TGG + chr2.2:58914794-58914813 MS.gene02029:CDS 55.0%
GCTACTGTGGCCAACTGCAT+TGG - chr2.2:58914792-58914811 None:intergenic 55.0%
! AGGTTTATCTGCGTGGGCTG+TGG + chr2.2:58915230-58915249 MS.gene02029:CDS 55.0%
! GGCACTGAGAGAGAGGATTC+TGG + chr2.2:58914704-58914723 MS.gene02029:CDS 55.0%
! GCGTGGGCTGTGGTGTTCCT+TGG + chr2.2:58915240-58915259 MS.gene02029:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 58913392 58915430 58913392 ID=MS.gene02029
chr2.2 mRNA 58913392 58915430 58913392 ID=MS.gene02029.t1;Parent=MS.gene02029
chr2.2 exon 58913392 58913826 58913392 ID=MS.gene02029.t1.exon1;Parent=MS.gene02029.t1
chr2.2 CDS 58913392 58913826 58913392 ID=cds.MS.gene02029.t1;Parent=MS.gene02029.t1
chr2.2 exon 58914666 58915430 58914666 ID=MS.gene02029.t1.exon2;Parent=MS.gene02029.t1
chr2.2 CDS 58914666 58915430 58914666 ID=cds.MS.gene02029.t1;Parent=MS.gene02029.t1
Gene Sequence

>MS.gene02029

GTTCAAGCTCTTTTAGATTATGAACCATTTGTTAATGCTGAGGAGGAGGAAGAAGATGAGTTTTGCAGTTGCTGTGAAGAAGATGCAGAAGAAAGAGATGAAGAAGAAAGAGAAGAAGAACAAGAAGAGTGGAAAGAAACTGAGGAGAAAGTGGTGGAAGGGTTGAAGGAAGAACTTGATGAATTTTCAGTAAGAATGTTCTTTAAGGGGTTATCAATTACTGGTGTTGAAAATTCAACTTCAGGGTTTTCTGGAATTGGAGTGTTTATGGAAAGATCATCAAATCTTCCTCCTATAAGGGTGCAGAAAAGGCTTGATTTTTATGCTGAGGAGCCTATGGTTGATTACTTGGCACTCATGGATGGGTTGTTAGAAGCTTTGCAGAACAAGATCCGTAAGGTTTATGCTTTCACTGATTCTGAGTTGTTATATAAGGTTAGTGAGTGATTCAGTAATTGATCTTAAGGTTTATTTGTTGTGACAATTACAATTTATGTATATTGATGAAGATTCTGACAAACCCTATAATTGTTAAATTGAAAATCACTTGTACTTTATTTATTTACTTTTTAGCATTACTCTGTTTTGATATACACTGATATACACCTTAGTTAAGCTCTTACAACAAAAGCTAATAAGCACTTATGTAAATATTATTTCTACAGCAACTGGTAAAATAAAGTCAAGTAGTTTTCGTATAAGATTAAGTTGTTTTCATAAGCTATCATGGCATGCTTATAGAAATAAACTTAAATGAGCGTATGCCTGTGTAATAGGTTGTTTTCATAAGCTCTCCCAAACATGTAATAAACAAACTCAAACAAGTCAATCCAAACAACCGTGTATGTCTGTTTAAAGTGTTGCTTGTTATTTTTGTCGTCAGTTATTTAGGTATGCTTCTCATGTTTTACTTTTGTGGATGAACTCTGAGGTAAGCGTTATAATATTTTCTATTGCTTACTCTAAAGTTTTGGTCAATTCTTGGGCGTGCTTAAACTAGCAATGGTATTTTCATTTCAAATTTTTTATTTTCTTATTGTAATTTGTATCTTGAATGGATATCATTTACTTCTGAATTCTGATTTCCTTGATTTGAATTTTCCTTTCTTTGGACCCCTTGTTTAGTTTCTTTAGCATATGATATTATTTTAAGAGGTTTGTGTTCTAGAAACCATGCTTTGACATAACCTCTATTAACTCTTAACTCTGTTTGTGTGTTTTTAGCATGCCCTTGTCTTTAACTATGTTTGTTTGAATGTGGATTTTTTTGACAGATAACAGCTGAGAAAAATTTGGACATGCCGCTTTTGATGGCACTGAGAGAGAGGATTCTGGAACATGCCAATAATTTTGAAACTTTTGATCTGAAGCTTATACCAAGCATTGATCTTGAGCAGCCAATGCAGTTGGCCACAGTAGCTATGGGACTCGTCACTTTTCCGGTAACTGGTGAAAAATTGCTCGAGAACTGTTCTATTTGTTGCGACGACAAGCCAGTGCCAATGATGATTACCTTGAAATGTTCACACACATTCTGTTCACATTGCTTGAGGTCCTATGCTGATGGTAAACTACAATGTTGTCAAGTCCCTATAAGGTGTCCTCAACCAGGATGCAGATATTGTATCTCTACACCCGAGTGCAAATCTTTTCTTCCATTCATCTCCTTCGAATCTCTCGAGAAAGCCCTTTCAGAAGCAAATATTGTCCAGTCAGAAAGATTTTATTGTCCATTTCTGAATTGTTCTGTTCTCCTTGATCCTTGCGAGTGTTTATCTGCAATGGACGGTTCATCTAGTCAATCGGACAATTCTTGTATCGAGTGTCCTGTTTGTCAGAGGTTTATCTGCGTGGGCTGTGGTGTTCCTTGGCATTCTTCCATGAGCTGTGAAGAATTTCAGAGTTTGCCGGAAGAGGAGAGAGATGCTTCTGACATTACCTTGCATCGTCTTGCTCAGAATAAAAGGTGGAAGCGCTGTCAGCAGTGTCGTATAATGATTGAGCTTACTCAAGGTTGTTACCACATGACATGCCGGTAA
Protein sequence

>MS.gene02029.t1

VQALLDYEPFVNAEEEEEDEFCSCCEEDAEERDEEEREEEQEEWKETEEKVVEGLKEELDEFSVRMFFKGLSITGVENSTSGFSGIGVFMERSSNLPPIRVQKRLDFYAEEPMVDYLALMDGLLEALQNKIRKVYAFTDSELLYKITAEKNLDMPLLMALRERILEHANNFETFDLKLIPSIDLEQPMQLATVAMGLVTFPVTGEKLLENCSICCDDKPVPMMITLKCSHTFCSHCLRSYADGKLQCCQVPIRCPQPGCRYCISTPECKSFLPFISFESLEKALSEANIVQSERFYCPFLNCSVLLDPCECLSAMDGSSSQSDNSCIECPVCQRFICVGCGVPWHSSMSCEEFQSLPEEERDASDITLHRLAQNKRWKRCQQCRIMIELTQGCYHMTCR