Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020895.t1 | XP_024638984.1 | 92.3 | 274 | 21 | 0 | 1 | 274 | 1 | 274 | 4.60E-145 | 523.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020895.t1 | Q9FMS6 | 59.9 | 277 | 104 | 4 | 3 | 272 | 1 | 277 | 4.2e-86 | 319.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020895.t1 | A0A396HXU6 | 92.3 | 274 | 21 | 0 | 1 | 274 | 1 | 274 | 3.3e-145 | 523.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene020895 | MS.gene025628 | PPI |
| MS.gene034250 | MS.gene020895 | PPI |
| MS.gene034816 | MS.gene020895 | PPI |
| MS.gene72977 | MS.gene020895 | PPI |
| MS.gene065709 | MS.gene020895 | PPI |
| MS.gene72978 | MS.gene020895 | PPI |
| MS.gene034815 | MS.gene020895 | PPI |
| MS.gene020895 | MS.gene21541 | PPI |
| MS.gene007154 | MS.gene020895 | PPI |
| MS.gene020895 | MS.gene41097 | PPI |
| MS.gene025629 | MS.gene020895 | PPI |
| MS.gene41100 | MS.gene020895 | PPI |
| MS.gene065707 | MS.gene020895 | PPI |
| MS.gene41101 | MS.gene020895 | PPI |
| MS.gene000609 | MS.gene020895 | PPI |
| MS.gene065708 | MS.gene020895 | PPI |
| MS.gene053225 | MS.gene020895 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020895.t1 | AT5G22250 | 59.928 | 277 | 104 | 4 | 3 | 272 | 1 | 277 | 2.58e-113 | 327 |
| MS.gene020895.t1 | AT3G44260 | 55.926 | 270 | 114 | 3 | 7 | 272 | 11 | 279 | 4.47e-105 | 306 |
| MS.gene020895.t1 | AT5G10960 | 46.667 | 270 | 140 | 4 | 4 | 272 | 1 | 267 | 1.76e-84 | 254 |
| MS.gene020895.t1 | AT2G32070 | 48.092 | 262 | 131 | 4 | 13 | 272 | 10 | 268 | 8.36e-82 | 247 |
| MS.gene020895.t1 | AT1G80780 | 46.743 | 261 | 135 | 4 | 13 | 272 | 10 | 267 | 9.42e-80 | 242 |
| MS.gene020895.t1 | AT1G80780 | 46.743 | 261 | 135 | 4 | 13 | 272 | 10 | 267 | 9.42e-80 | 242 |
| MS.gene020895.t1 | AT1G80780 | 46.743 | 261 | 135 | 4 | 13 | 272 | 10 | 267 | 1.32e-79 | 242 |
| MS.gene020895.t1 | AT1G15920 | 46.154 | 273 | 135 | 6 | 7 | 271 | 8 | 276 | 4.83e-76 | 233 |
| MS.gene020895.t1 | AT1G15920 | 46.154 | 273 | 135 | 6 | 7 | 271 | 8 | 276 | 4.83e-76 | 233 |
| MS.gene020895.t1 | AT1G61470 | 36.777 | 242 | 136 | 6 | 14 | 251 | 1 | 229 | 7.47e-41 | 142 |
| MS.gene020895.t1 | AT1G06450 | 34.426 | 244 | 147 | 6 | 14 | 254 | 8 | 241 | 1.86e-37 | 135 |
| MS.gene020895.t1 | AT3G44240 | 34.061 | 229 | 138 | 6 | 28 | 254 | 2 | 219 | 3.37e-34 | 124 |
| MS.gene020895.t1 | AT1G27820 | 34.000 | 250 | 140 | 9 | 11 | 251 | 2 | 235 | 5.79e-34 | 125 |
| MS.gene020895.t1 | AT1G27890 | 33.471 | 242 | 140 | 8 | 17 | 251 | 3 | 230 | 1.38e-31 | 119 |
Find 58 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTACTCTAATGCACTTTA+TGG | 0.122182 | 5.4:+76627366 | MS.gene020895:CDS |
| TTCCACAGTGCGTATGATTT+TGG | 0.151783 | 5.4:+76627231 | MS.gene020895:CDS |
| CTCAATGGACACTGAATTTC+CGG | 0.199766 | 5.4:+76626864 | MS.gene020895:CDS |
| AATTGTATCCCCACACTTTA+CGG | 0.267621 | 5.4:-76626789 | None:intergenic |
| TATTGACTTGCTCCGCCGTC+AGG | 0.317248 | 5.4:+76627101 | MS.gene020895:CDS |
| CAAGTAACTGTATTGCTCAT+TGG | 0.320243 | 5.4:-76626926 | None:intergenic |
| TCAGATCGGTCTTACCTTAT+CGG | 0.320555 | 5.4:+76626978 | MS.gene020895:CDS |
| TCCGTGACTTTAACGTCAAC+AGG | 0.331875 | 5.4:+76627058 | MS.gene020895:CDS |
| AAGTAACTGTATTGCTCATT+GGG | 0.348772 | 5.4:-76626925 | None:intergenic |
| ATGAGAACAGTCGCTACATC+TGG | 0.354062 | 5.4:+76627025 | MS.gene020895:CDS |
| TTCTTTCGCAACACTGTTCA+AGG | 0.358495 | 5.4:+76627132 | MS.gene020895:CDS |
| CACACTTTACGGATCATGAT+TGG | 0.401574 | 5.4:-76626778 | None:intergenic |
| TAAATACAATTTCATCTCAA+TGG | 0.417841 | 5.4:+76626849 | MS.gene020895:CDS |
| AACTGTCGGATCCAGCTTGA+TGG | 0.430459 | 5.4:-76627443 | None:intergenic |
| GCTACGCTGATGCGGTGGTC+TGG | 0.430872 | 5.4:+76627174 | MS.gene020895:CDS |
| AAGCACGCTGGATCAATATT+CGG | 0.452737 | 5.4:+76627528 | MS.gene020895:CDS |
| CGAGTATTCTTCAAGTGGAT+CGG | 0.453450 | 5.4:+76627406 | MS.gene020895:CDS |
| AACTGTATTGCTCATTGGGT+TGG | 0.457601 | 5.4:-76626921 | None:intergenic |
| CTTACCTTATCGGATGCAAT+AGG | 0.458429 | 5.4:+76626988 | MS.gene020895:CDS |
| TTGGAGGCGACGACGGATTG+TGG | 0.464331 | 5.4:-76626902 | None:intergenic |
| TGTATTGCTCATTGGGTTGG+AGG | 0.469216 | 5.4:-76626918 | None:intergenic |
| ATTCAATATCATTCGTCAGT+TGG | 0.476456 | 5.4:+76626822 | MS.gene020895:CDS |
| CTTCAAGTGGATCGGGCCAT+TGG | 0.478735 | 5.4:+76627414 | MS.gene020895:CDS |
| TGGAGGCGACGACGGATTGT+GGG | 0.482460 | 5.4:-76626901 | None:intergenic |
| TCAATGGACACTGAATTTCC+GGG | 0.492924 | 5.4:+76626865 | MS.gene020895:CDS |
| GAGTATTCTTCAAGTGGATC+GGG | 0.498516 | 5.4:+76627407 | MS.gene020895:CDS |
| AATATCATTCGTCAGTTGGT+TGG | 0.513084 | 5.4:+76626826 | MS.gene020895:CDS |
| TGAGCAATACAGTTACTTGA+AGG | 0.514649 | 5.4:+76626930 | MS.gene020895:CDS |
| GTTACCTATTGCATCCGATA+AGG | 0.524193 | 5.4:-76626992 | None:intergenic |
| ATTGGTAAGTGCCATCAAGC+TGG | 0.527440 | 5.4:+76627432 | MS.gene020895:CDS |
| ATTGACTTGCTCCGCCGTCA+GGG | 0.527736 | 5.4:+76627102 | MS.gene020895:CDS |
| GACTGCAATGATAATGATGA+AGG | 0.560933 | 5.4:+76626738 | None:intergenic |
| GACGACGGATTGTGGGTGAA+TGG | 0.565974 | 5.4:-76626894 | None:intergenic |
| GTGGGTGAATGGACGATGCC+CGG | 0.568102 | 5.4:-76626883 | None:intergenic |
| CCCTGTTGACGTTAAAGTCA+CGG | 0.570625 | 5.4:-76627059 | None:intergenic |
| CAATAGAGTCTTTGTTGTGG+AGG | 0.575807 | 5.4:-76627083 | None:intergenic |
| CATTGGGTTGGAGGCGACGA+CGG | 0.576634 | 5.4:-76626909 | None:intergenic |
| AATCATGATCCGTAAAGTGT+GGG | 0.578633 | 5.4:+76626780 | MS.gene020895:CDS |
| AGTTGCGAGTATTCTTCAAG+TGG | 0.590018 | 5.4:+76627401 | MS.gene020895:CDS |
| CAATCATGATCCGTAAAGTG+TGG | 0.590475 | 5.4:+76626779 | MS.gene020895:CDS |
| TGAGAACAGTCGCTACATCT+GGG | 0.592809 | 5.4:+76627026 | MS.gene020895:CDS |
| TAGCGACTGTTCTCATCATG+AGG | 0.601877 | 5.4:-76627018 | None:intergenic |
| TTTCAACAACTCAGTGACAT+GGG | 0.602278 | 5.4:+76627203 | MS.gene020895:CDS |
| TACTCTAATGCACTTTATGG+CGG | 0.605426 | 5.4:+76627369 | MS.gene020895:CDS |
| AGCTTGATGGCACTTACCAA+TGG | 0.623590 | 5.4:-76627430 | None:intergenic |
| CCGTGACTTTAACGTCAACA+GGG | 0.626499 | 5.4:+76627059 | MS.gene020895:CDS |
| AGTCAATAGAGTCTTTGTTG+TGG | 0.637942 | 5.4:-76627086 | None:intergenic |
| CATAAAGTGCATTAGAGTAG+CGG | 0.649775 | 5.4:-76627365 | None:intergenic |
| GACTGTTCTCATCATGAGGG+AGG | 0.654467 | 5.4:-76627014 | None:intergenic |
| GATATTGAGAGCGTCAACGT+TGG | 0.662239 | 5.4:-76626953 | None:intergenic |
| ATCATGATCCGTAAAGTGTG+GGG | 0.662913 | 5.4:+76626781 | MS.gene020895:CDS |
| AGCGACTGTTCTCATCATGA+GGG | 0.665310 | 5.4:-76627017 | None:intergenic |
| ATCCAAAATCATACGCACTG+TGG | 0.668840 | 5.4:-76627233 | None:intergenic |
| GCTCTCAATATCATTCAGAT+CGG | 0.674471 | 5.4:+76626964 | MS.gene020895:CDS |
| CTGCGACGTCAACAAACTGT+CGG | 0.689188 | 5.4:-76627457 | None:intergenic |
| ATCTGGTGAAAATCTTAACG+CGG | 0.715080 | 5.4:+76627256 | MS.gene020895:CDS |
| GAAAGAAGTCGATACCCTGA+CGG | 0.733589 | 5.4:-76627116 | None:intergenic |
| AGAAGTCGATACCCTGACGG+CGG | 0.736815 | 5.4:-76627113 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATACAATTTCATCTCAA+TGG | + | chr5.4:76626849-76626868 | MS.gene020895:CDS | 20.0% |
| !!! | CATGTTTTGAGAAAATTATT+TGG | + | chr5.4:76627312-76627331 | MS.gene020895:CDS | 20.0% |
| AAGTAACTGTATTGCTCATT+GGG | - | chr5.4:76626928-76626947 | None:intergenic | 30.0% | |
| ATTCAATATCATTCGTCAGT+TGG | + | chr5.4:76626822-76626841 | MS.gene020895:CDS | 30.0% | |
| !!! | ATCATGATTGGTTTTGAACT+AGG | - | chr5.4:76626769-76626788 | None:intergenic | 30.0% |
| AAAAACTCTTTCAAGCTACG+AGG | - | chr5.4:76627291-76627310 | None:intergenic | 35.0% | |
| AATATCATTCGTCAGTTGGT+TGG | + | chr5.4:76626826-76626845 | MS.gene020895:CDS | 35.0% | |
| AATCATGATCCGTAAAGTGT+GGG | + | chr5.4:76626780-76626799 | MS.gene020895:CDS | 35.0% | |
| AATTGTATCCCCACACTTTA+CGG | - | chr5.4:76626792-76626811 | None:intergenic | 35.0% | |
| AGTCAATAGAGTCTTTGTTG+TGG | - | chr5.4:76627089-76627108 | None:intergenic | 35.0% | |
| ATCTGGTGAAAATCTTAACG+CGG | + | chr5.4:76627256-76627275 | MS.gene020895:CDS | 35.0% | |
| CAAGTAACTGTATTGCTCAT+TGG | - | chr5.4:76626929-76626948 | None:intergenic | 35.0% | |
| CATAAAGTGCATTAGAGTAG+CGG | - | chr5.4:76627368-76627387 | None:intergenic | 35.0% | |
| GCTCTCAATATCATTCAGAT+CGG | + | chr5.4:76626964-76626983 | MS.gene020895:CDS | 35.0% | |
| TACTCTAATGCACTTTATGG+CGG | + | chr5.4:76627369-76627388 | MS.gene020895:CDS | 35.0% | |
| TGAGCAATACAGTTACTTGA+AGG | + | chr5.4:76626930-76626949 | MS.gene020895:CDS | 35.0% | |
| TTTCAACAACTCAGTGACAT+GGG | + | chr5.4:76627203-76627222 | MS.gene020895:CDS | 35.0% | |
| ! | TGCGTATGATTTTGGATATC+TGG | + | chr5.4:76627239-76627258 | MS.gene020895:CDS | 35.0% |
| ! | TTTTCAACAACTCAGTGACA+TGG | + | chr5.4:76627202-76627221 | MS.gene020895:CDS | 35.0% |
| AACTGTATTGCTCATTGGGT+TGG | - | chr5.4:76626924-76626943 | None:intergenic | 40.0% | |
| AAGCACGCTGGATCAATATT+CGG | + | chr5.4:76627528-76627547 | MS.gene020895:CDS | 40.0% | |
| AGTTGCGAGTATTCTTCAAG+TGG | + | chr5.4:76627401-76627420 | MS.gene020895:CDS | 40.0% | |
| ATCATGATCCGTAAAGTGTG+GGG | + | chr5.4:76626781-76626800 | MS.gene020895:CDS | 40.0% | |
| ATCCAAAATCATACGCACTG+TGG | - | chr5.4:76627236-76627255 | None:intergenic | 40.0% | |
| CAATCATGATCCGTAAAGTG+TGG | + | chr5.4:76626779-76626798 | MS.gene020895:CDS | 40.0% | |
| CACACTTTACGGATCATGAT+TGG | - | chr5.4:76626781-76626800 | None:intergenic | 40.0% | |
| CGAGTATTCTTCAAGTGGAT+CGG | + | chr5.4:76627406-76627425 | MS.gene020895:CDS | 40.0% | |
| CGCTACTCTAATGCACTTTA+TGG | + | chr5.4:76627366-76627385 | MS.gene020895:CDS | 40.0% | |
| CTCAATGGACACTGAATTTC+CGG | + | chr5.4:76626864-76626883 | MS.gene020895:CDS | 40.0% | |
| CTTACCTTATCGGATGCAAT+AGG | + | chr5.4:76626988-76627007 | MS.gene020895:CDS | 40.0% | |
| GAGTATTCTTCAAGTGGATC+GGG | + | chr5.4:76627407-76627426 | MS.gene020895:CDS | 40.0% | |
| GTTACCTATTGCATCCGATA+AGG | - | chr5.4:76626995-76627014 | None:intergenic | 40.0% | |
| TCAATGGACACTGAATTTCC+GGG | + | chr5.4:76626865-76626884 | MS.gene020895:CDS | 40.0% | |
| TCAGATCGGTCTTACCTTAT+CGG | + | chr5.4:76626978-76626997 | MS.gene020895:CDS | 40.0% | |
| TTCTTTCGCAACACTGTTCA+AGG | + | chr5.4:76627132-76627151 | MS.gene020895:CDS | 40.0% | |
| ! | AATGAAGTCAAAAAGCACGC+TGG | + | chr5.4:76627516-76627535 | MS.gene020895:CDS | 40.0% |
| ! | CAATAGAGTCTTTGTTGTGG+AGG | - | chr5.4:76627086-76627105 | None:intergenic | 40.0% |
| ! | TTCATTTTGCTACGCTGATG+CGG | + | chr5.4:76627166-76627185 | MS.gene020895:CDS | 40.0% |
| ! | TTCCACAGTGCGTATGATTT+TGG | + | chr5.4:76627231-76627250 | MS.gene020895:CDS | 40.0% |
| AGCGACTGTTCTCATCATGA+GGG | - | chr5.4:76627020-76627039 | None:intergenic | 45.0% | |
| ATGAGAACAGTCGCTACATC+TGG | + | chr5.4:76627025-76627044 | MS.gene020895:CDS | 45.0% | |
| ATTGGTAAGTGCCATCAAGC+TGG | + | chr5.4:76627432-76627451 | MS.gene020895:CDS | 45.0% | |
| CCCTGTTGACGTTAAAGTCA+CGG | - | chr5.4:76627062-76627081 | None:intergenic | 45.0% | |
| CCGTGACTTTAACGTCAACA+GGG | + | chr5.4:76627059-76627078 | MS.gene020895:CDS | 45.0% | |
| GAAAGAAGTCGATACCCTGA+CGG | - | chr5.4:76627119-76627138 | None:intergenic | 45.0% | |
| TAGCGACTGTTCTCATCATG+AGG | - | chr5.4:76627021-76627040 | None:intergenic | 45.0% | |
| TCCGTGACTTTAACGTCAAC+AGG | + | chr5.4:76627058-76627077 | MS.gene020895:CDS | 45.0% | |
| TGAGAACAGTCGCTACATCT+GGG | + | chr5.4:76627026-76627045 | MS.gene020895:CDS | 45.0% | |
| ! | AGCTTGATGGCACTTACCAA+TGG | - | chr5.4:76627433-76627452 | None:intergenic | 45.0% |
| ! | GTCGCAGACTTTTCACAAGA+TGG | + | chr5.4:76627473-76627492 | MS.gene020895:CDS | 45.0% |
| !! | GATATTGAGAGCGTCAACGT+TGG | - | chr5.4:76626956-76626975 | None:intergenic | 45.0% |
| !! | TGTATTGCTCATTGGGTTGG+AGG | - | chr5.4:76626921-76626940 | None:intergenic | 45.0% |
| AACTGTCGGATCCAGCTTGA+TGG | - | chr5.4:76627446-76627465 | None:intergenic | 50.0% | |
| CTGCGACGTCAACAAACTGT+CGG | - | chr5.4:76627460-76627479 | None:intergenic | 50.0% | |
| GACTGTTCTCATCATGAGGG+AGG | - | chr5.4:76627017-76627036 | None:intergenic | 50.0% | |
| ! | ATTTTGCTACGCTGATGCGG+TGG | + | chr5.4:76627169-76627188 | MS.gene020895:CDS | 50.0% |
| AGAAGTCGATACCCTGACGG+CGG | - | chr5.4:76627116-76627135 | None:intergenic | 55.0% | |
| CTTCAAGTGGATCGGGCCAT+TGG | + | chr5.4:76627414-76627433 | MS.gene020895:CDS | 55.0% | |
| !! | ATTGACTTGCTCCGCCGTCA+GGG | + | chr5.4:76627102-76627121 | MS.gene020895:CDS | 55.0% |
| !! | GACGACGGATTGTGGGTGAA+TGG | - | chr5.4:76626897-76626916 | None:intergenic | 55.0% |
| !! | TATTGACTTGCTCCGCCGTC+AGG | + | chr5.4:76627101-76627120 | MS.gene020895:CDS | 55.0% |
| GTGGGTGAATGGACGATGCC+CGG | - | chr5.4:76626886-76626905 | None:intergenic | 60.0% | |
| !! | CATTGGGTTGGAGGCGACGA+CGG | - | chr5.4:76626912-76626931 | None:intergenic | 60.0% |
| !! | TGGAGGCGACGACGGATTGT+GGG | - | chr5.4:76626904-76626923 | None:intergenic | 60.0% |
| !! | TTGGAGGCGACGACGGATTG+TGG | - | chr5.4:76626905-76626924 | None:intergenic | 60.0% |
| GCTACGCTGATGCGGTGGTC+TGG | + | chr5.4:76627174-76627193 | MS.gene020895:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 76626745 | 76627569 | 76626745 | ID=MS.gene020895 |
| chr5.4 | mRNA | 76626745 | 76627569 | 76626745 | ID=MS.gene020895.t1;Parent=MS.gene020895 |
| chr5.4 | exon | 76626745 | 76627569 | 76626745 | ID=MS.gene020895.t1.exon1;Parent=MS.gene020895.t1 |
| chr5.4 | CDS | 76626745 | 76627569 | 76626745 | ID=cds.MS.gene020895.t1;Parent=MS.gene020895.t1 |
| Gene Sequence |
| Protein sequence |
