AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025825

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025825.t1 MTR_5g012810 98.780 246 3 0 1 246 1 246 6.99e-167 461
MS.gene025825.t1 MTR_8g013680 78.313 249 52 2 1 248 1 248 2.13e-131 371
MS.gene025825.t1 MTR_2g101370 76.230 244 57 1 4 246 5 248 2.03e-124 353
MS.gene025825.t1 MTR_4g063090 62.832 226 81 2 24 246 26 251 2.44e-91 270
MS.gene025825.t1 MTR_7g103030 62.449 245 91 1 3 246 5 249 6.10e-90 266
MS.gene025825.t1 MTR_1g115405 55.061 247 108 2 3 247 5 250 1.60e-89 265
MS.gene025825.t1 MTR_1g006490 54.812 239 103 3 1 238 1 235 3.42e-83 249
MS.gene025825.t1 MTR_7g023350 54.878 246 110 1 3 247 5 250 1.18e-79 240
MS.gene025825.t1 MTR_1g077540 50.833 240 117 1 3 241 5 244 1.54e-78 237
MS.gene025825.t1 MTR_1g006590 52.610 249 110 5 1 246 1 244 1.58e-78 237
MS.gene025825.t1 MTR_6g069040 53.333 240 111 1 3 241 5 244 3.59e-73 224
MS.gene025825.t1 MTR_2g060360 47.807 228 117 2 9 235 9 235 4.35e-69 213
MS.gene025825.t1 MTR_5g063930 38.866 247 133 8 8 248 26 260 4.82e-40 139
MS.gene025825.t1 MTR_2g094270 38.153 249 134 7 9 238 28 275 1.79e-38 135
MS.gene025825.t1 MTR_7g101190 39.301 229 122 5 19 231 39 266 7.69e-38 134
MS.gene025825.t1 MTR_4g059390 37.712 236 130 5 19 238 39 273 4.07e-36 129
MS.gene025825.t1 MTR_3g118010 39.631 217 116 5 19 223 37 250 7.14e-36 129
MS.gene025825.t1 MTR_3g070210 35.398 226 130 5 19 231 55 277 6.05e-31 116
MS.gene025825.t1 MTR_5g082070 35.841 226 129 5 19 231 55 277 3.39e-30 114
MS.gene025825.t1 MTR_2g094270 37.788 217 118 6 9 209 28 243 2.28e-29 111
MS.gene025825.t1 MTR_5g010150 37.168 226 126 6 19 231 52 274 4.87e-29 111
MS.gene025825.t1 MTR_8g098375 36.283 226 128 7 19 231 52 274 1.16e-28 110
MS.gene025825.t1 MTR_4g415300 36.889 225 127 6 19 231 56 277 1.22e-28 110
MS.gene025825.t1 MTR_1g095070 39.548 177 94 4 67 231 25 200 1.03e-27 106
MS.gene025825.t1 MTR_2g017620 35.874 223 131 7 3 223 10 222 3.07e-27 105
MS.gene025825.t1 MTR_2g017620 35.874 223 131 7 3 223 24 236 4.64e-27 105
MS.gene025825.t1 MTR_3g008920 36.170 235 136 7 15 247 22 244 5.87e-27 105
MS.gene025825.t1 MTR_2g017660 34.167 240 141 8 14 249 34 260 2.98e-26 103
MS.gene025825.t1 MTR_2g017590 33.921 227 139 6 3 228 22 238 3.45e-26 103
MS.gene025825.t1 MTR_4g084880 34.052 232 140 6 18 246 34 255 4.41e-26 103
MS.gene025825.t1 MTR_7g010650 34.272 213 133 3 19 231 37 242 9.51e-26 102
MS.gene025825.t1 MTR_8g087710 32.068 237 144 6 14 246 34 257 1.09e-25 102
MS.gene025825.t1 MTR_5g079570 33.610 241 141 7 14 248 35 262 1.38e-25 101
MS.gene025825.t1 MTR_2g104940 35.749 207 114 5 23 223 84 277 2.61e-25 101
MS.gene025825.t1 MTR_4g006730 35.122 205 118 4 23 223 79 272 5.31e-25 100
MS.gene025825.t1 MTR_8g087720 32.365 241 149 5 10 246 26 256 1.13e-24 99.4
MS.gene025825.t1 MTR_2g017610 32.174 230 145 7 18 246 37 256 3.45e-23 95.5
MS.gene025825.t1 MTR_1g097840 36.792 212 114 6 23 228 78 275 5.39e-23 95.5
MS.gene025825.t1 MTR_2g017620 41.333 150 83 4 76 223 9 155 8.26e-23 92.4
MS.gene025825.t1 MTR_3g070210 34.343 198 117 4 19 206 55 249 8.46e-23 94.4
MS.gene025825.t1 MTR_1g070385 33.649 211 128 6 22 230 65 265 1.21e-22 97.1
MS.gene025825.t1 MTR_2g017570 31.739 230 146 7 18 246 37 256 1.28e-22 94.0
MS.gene025825.t1 MTR_4g006630 34.184 196 114 4 32 223 1 185 3.87e-22 91.3
MS.gene025825.t1 MTR_4g006730 34.184 196 114 4 32 223 1 185 4.12e-22 91.3
MS.gene025825.t1 MTR_4g059390 35.928 167 97 3 19 176 39 204 1.43e-20 86.7
MS.gene025825.t1 MTR_1g095070 39.597 149 80 3 67 206 25 172 1.52e-20 86.3
MS.gene025825.t1 MTR_4g075570 32.129 249 137 8 11 237 43 281 1.58e-16 77.8
MS.gene025825.t1 MTR_3g070210 31.902 163 102 3 19 175 55 214 3.10e-16 75.5
MS.gene025825.t1 MTR_4g415300 34.463 177 106 5 19 190 56 227 4.55e-15 72.4
MS.gene025825.t1 MTR_5g010150 34.756 164 98 4 19 176 52 212 7.72e-15 71.6
MS.gene025825.t1 MTR_8g098375 32.335 167 104 4 19 179 52 215 1.09e-14 71.6
MS.gene025825.t1 MTR_8g468730 39.604 101 41 4 143 242 8 89 1.19e-12 62.8
MS.gene025825.t1 MTR_4g075590 28.512 242 143 8 11 231 29 261 1.63e-12 66.2
MS.gene025825.t1 MTR_4g006730 33.529 170 98 4 23 188 79 237 3.11e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025825.t1 AT5G47450 79.757 247 49 1 1 246 1 247 2.88e-137 386
MS.gene025825.t1 AT4G17340 82.800 250 42 1 1 249 1 250 1.37e-133 377
MS.gene025825.t1 AT3G16240 76.639 244 56 1 1 243 1 244 1.68e-129 366
MS.gene025825.t1 AT3G26520 64.069 231 82 1 7 236 10 240 6.34e-99 289
MS.gene025825.t1 AT2G36830 61.538 247 94 1 3 248 5 251 2.51e-98 287
MS.gene025825.t1 AT4G01470 55.466 247 107 2 3 246 5 251 2.30e-88 262
MS.gene025825.t1 AT1G73190 53.909 243 106 2 5 241 10 252 3.07e-76 232
MS.gene025825.t1 AT1G17810 53.600 250 110 2 5 248 10 259 3.32e-76 232
MS.gene025825.t1 AT2G25810 54.000 250 110 3 1 249 1 246 5.39e-76 231
MS.gene025825.t1 AT3G47440 45.798 238 126 3 1 236 4 240 7.60e-66 205
MS.gene025825.t1 AT5G60660 38.865 229 123 6 19 231 39 266 4.89e-37 132
MS.gene025825.t1 AT2G37170 38.306 248 134 8 9 238 68 314 7.86e-37 132
MS.gene025825.t1 AT2G37170 38.095 252 137 8 9 242 25 275 8.58e-37 131
MS.gene025825.t1 AT2G37180 37.860 243 134 6 19 245 37 278 1.77e-36 130
MS.gene025825.t1 AT4G35100 41.364 220 108 7 19 223 38 251 4.85e-36 129
MS.gene025825.t1 AT4G35100 41.364 220 108 7 19 223 38 251 4.85e-36 129
MS.gene025825.t1 AT3G53420 39.419 241 127 7 19 242 39 277 7.92e-36 129
MS.gene025825.t1 AT3G53420 39.419 241 127 7 19 242 39 277 7.92e-36 129
MS.gene025825.t1 AT3G54820 36.145 249 133 7 19 243 38 284 1.70e-35 128
MS.gene025825.t1 AT2G39010 35.802 243 133 6 11 231 24 265 1.23e-34 125
MS.gene025825.t1 AT2G16850 39.631 217 116 5 19 223 36 249 1.42e-33 122
MS.gene025825.t1 AT4G00430 37.333 225 126 5 19 231 53 274 3.04e-30 114
MS.gene025825.t1 AT4G23400 36.889 225 127 5 19 231 53 274 3.71e-30 114
MS.gene025825.t1 AT3G61430 36.444 225 128 5 19 231 52 273 5.58e-30 113
MS.gene025825.t1 AT3G61430 36.444 225 128 5 19 231 52 273 5.58e-30 113
MS.gene025825.t1 AT1G52180 60.674 89 35 0 123 211 34 122 9.36e-30 108
MS.gene025825.t1 AT1G01620 36.889 225 127 5 19 231 52 273 1.06e-29 112
MS.gene025825.t1 AT2G45960 35.556 225 130 5 19 231 52 273 7.51e-29 110
MS.gene025825.t1 AT1G01620 39.153 189 102 4 55 231 14 201 1.04e-26 103
MS.gene025825.t1 AT4G18910 33.745 243 143 7 12 246 45 277 6.27e-26 103
MS.gene025825.t1 AT5G37810 33.913 230 139 6 19 246 43 261 9.09e-26 102
MS.gene025825.t1 AT1G80760 33.043 230 135 5 23 246 84 300 1.86e-25 102
MS.gene025825.t1 AT4G19030 32.099 243 147 6 12 246 48 280 2.03e-25 101
MS.gene025825.t1 AT5G37820 34.545 220 124 6 19 231 43 249 1.80e-24 99.0
MS.gene025825.t1 AT5G37820 33.333 231 139 6 19 246 79 297 3.17e-24 99.0
MS.gene025825.t1 AT3G06100 31.878 229 150 3 6 234 33 255 3.43e-24 98.2
MS.gene025825.t1 AT1G31885 32.653 245 141 5 5 232 20 257 4.31e-24 98.6
MS.gene025825.t1 AT3G06100 32.864 213 137 3 22 234 1 207 1.41e-22 92.8
MS.gene025825.t1 AT4G10380 32.895 228 132 6 6 223 59 275 2.13e-21 91.3
MS.gene025825.t1 AT1G31885 34.762 210 122 4 31 232 1 203 3.51e-21 89.7
MS.gene025825.t1 AT2G45960 35.025 197 116 4 19 206 52 245 6.03e-21 89.4
MS.gene025825.t1 AT2G45960 35.025 197 116 4 19 206 52 245 7.83e-21 89.7
MS.gene025825.t1 AT2G39010 32.222 180 106 4 11 175 24 202 2.71e-18 81.3
MS.gene025825.t1 AT2G34390 32.599 227 137 6 13 231 42 260 1.27e-17 80.5
MS.gene025825.t1 AT2G34390 33.028 218 130 6 22 231 9 218 1.09e-16 77.4
MS.gene025825.t1 AT3G06100 35.606 132 84 1 103 234 44 174 1.74e-15 72.8
MS.gene025825.t1 AT4G00430 34.783 161 97 3 19 174 53 210 5.71e-15 72.0

Find 81 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTGGCTGGGTTGATTTA+TGG 0.208743 5.3:+5959837 MS.gene025825:CDS
TTGCTGATAACTGGATCTAT+TGG 0.258320 5.3:+5959793 MS.gene025825:CDS
TCCTAACTGGTCTCTTCTAT+TGG 0.261693 5.3:+5959138 MS.gene025825:CDS
ATTTATGGTGACGTCTTTAT+TGG 0.274341 5.3:+5959852 MS.gene025825:CDS
CACAGCAGCTGACCCCAAAA+AGG 0.277234 5.3:+5959638 MS.gene025825:CDS
TTGAACCCAGCTGTTACTTT+TGG 0.284387 5.3:+5959089 MS.gene025825:CDS
ACCATTGCACCGATTGCTAT+TGG 0.294639 5.3:+5959672 MS.gene025825:CDS
GTGGAAACTTTGCTGATAAC+TGG 0.301786 5.3:+5959784 MS.gene025825:CDS
TTTGAGATTATCATAACATT+TGG 0.330146 5.3:+5959594 MS.gene025825:CDS
TCAAACACTAATCCAGCAAT+AGG 0.335099 5.3:-5959576 None:intergenic
ACCTGAGATGTTTGCTGCTA+TGG 0.342340 5.3:-5959060 None:intergenic
ATGAACCCGGCTCGCTCATT+CGG 0.368228 5.3:+5959747 MS.gene025825:CDS
CCATTGCACCGATTGCTATT+GGG 0.370396 5.3:+5959673 MS.gene025825:CDS
TATTGGGTTGGACCATTGAT+AGG 0.371182 5.3:+5959810 MS.gene025825:CDS
TGCTGATAACTGGATCTATT+GGG 0.375661 5.3:+5959794 MS.gene025825:CDS
AAAATGGTGAAGATAGGTTT+TGG 0.375817 5.3:+5957993 None:intergenic
CATGCATTTGCACTATTTGT+TGG 0.376412 5.3:+5959032 MS.gene025825:CDS
TATGTTTCAGATGCTGGTGC+TGG 0.377613 5.3:-5959885 None:intergenic
TTGATAGGAGGAGGCTTGGC+TGG 0.378621 5.3:+5959825 MS.gene025825:CDS
TATTGGATTGCCCAATTGCT+TGG 0.382330 5.3:+5959155 MS.gene025825:CDS
CAACGATGGAGCCAAGCAAT+TGG 0.397490 5.3:-5959166 None:intergenic
CCAACCCATGGAGTGGCTGC+TGG 0.411983 5.3:+5959546 MS.gene025825:CDS
TAAGGGTATGTTTCAGATGC+TGG 0.414974 5.3:-5959891 None:intergenic
CTCTGATAAATAAGCCTTGA+GGG 0.415032 5.3:-5958045 None:intergenic
GGCAACATCACCATCCTAAC+TGG 0.420270 5.3:+5959125 MS.gene025825:CDS
GCTGGACTGAACCCTATTGC+TGG 0.425119 5.3:+5959564 MS.gene025825:CDS
ATGTTTCAGATGCTGGTGCT+GGG 0.433498 5.3:-5959884 None:intergenic
CTTGGATCCAGCGGGTTTAG+TGG 0.446191 5.3:+5958995 MS.gene025825:CDS
TTCGGCCCAGCTGTGGTTAG+TGG 0.448499 5.3:+5959765 MS.gene025825:CDS
TCAGTCCAGCAGCCACTCCA+TGG 0.457656 5.3:-5959551 None:intergenic
CTGGGCCGAATGAGCGAGCC+GGG 0.457837 5.3:-5959752 None:intergenic
ATTGCCAAAATGGTGAAGAT+AGG 0.457933 5.3:+5957987 None:intergenic
ATTGCTATTGGGTTCGTTGT+CGG 0.459779 5.3:+5959684 MS.gene025825:CDS
AAGTTTCCACTAACCACAGC+TGG 0.477936 5.3:-5959771 None:intergenic
GATTATCATAACATTTGGAT+TGG 0.481301 5.3:+5959599 MS.gene025825:CDS
GCTAATCCAAAAGTAACAGC+TGG 0.483137 5.3:-5959095 None:intergenic
TTCATTGAACCTCCACTGAA+TGG 0.492137 5.3:-5959729 None:intergenic
GCCATAGCAGCAAACATCTC+AGG 0.493649 5.3:+5959059 MS.gene025825:CDS
AACGATGGAGCCAAGCAATT+GGG 0.495370 5.3:-5959165 None:intergenic
TGATAGGAGGAGGCTTGGCT+GGG 0.495930 5.3:+5959826 MS.gene025825:CDS
ACCATTGATAGGAGGAGGCT+TGG 0.499184 5.3:+5959821 MS.gene025825:CDS
CAAACACTAATCCAGCAATA+GGG 0.503864 5.3:-5959575 None:intergenic
CACTAAACCCGCTGGATCCA+AGG 0.511849 5.3:-5958994 None:intergenic
GCCAAGCCTCCTCCTATCAA+TGG 0.517459 5.3:-5959822 None:intergenic
TCTAACATCAGATGCAGCCT+TGG 0.521701 5.3:+5958977 MS.gene025825:CDS
CCCAATAGCAATCGGTGCAA+TGG 0.522337 5.3:-5959673 None:intergenic
GTTGGACCATTGATAGGAGG+AGG 0.537767 5.3:+5959816 MS.gene025825:CDS
TTGCAGAGTGTTCCAACCCA+TGG 0.540788 5.3:+5959534 MS.gene025825:intron
ATTTGCACTATTTGTTGGTG+TGG 0.541154 5.3:+5959037 MS.gene025825:CDS
GCTGGGCCGAATGAGCGAGC+CGG 0.544156 5.3:-5959753 None:intergenic
CAGTGGAGGTTCAATGAACC+CGG 0.550196 5.3:+5959734 MS.gene025825:CDS
CAGTCCAGCAGCCACTCCAT+GGG 0.550781 5.3:-5959550 None:intergenic
TGAACCTCCACTGAATGGAC+CGG 0.561117 5.3:-5959724 None:intergenic
CCAGCAGCCACTCCATGGGT+TGG 0.562685 5.3:-5959546 None:intergenic
TCCAATAGAAGAGACCAGTT+AGG 0.566741 5.3:-5959139 None:intergenic
ATTTAGCATTGCCTCCCTCA+AGG 0.567116 5.3:+5958031 MS.gene025825:CDS
TCGCTCATTCGGCCCAGCTG+TGG 0.568998 5.3:+5959758 MS.gene025825:CDS
ACAACGAACCCAATAGCAAT+CGG 0.576808 5.3:-5959681 None:intergenic
GATAACTGGATCTATTGGGT+TGG 0.583637 5.3:+5959798 MS.gene025825:CDS
CCTTAGCGGTGACATAATTG+AGG 0.583882 5.3:-5959199 None:intergenic
CCTCAATTATGTCACCGCTA+AGG 0.583887 5.3:+5959199 MS.gene025825:CDS
GATGCAGCCTTGGATCCAGC+GGG 0.590239 5.3:+5958987 MS.gene025825:CDS
GCTACTGCCACTAAACCCGC+TGG 0.595890 5.3:-5959002 None:intergenic
GATGGTGATGTTGCCTCCAA+TGG 0.596576 5.3:-5959117 None:intergenic
ATAGAAGAGACCAGTTAGGA+TGG 0.597084 5.3:-5959135 None:intergenic
GGGTTTAGTGGCAGTAGCTG+TGG 0.598717 5.3:+5959007 MS.gene025825:CDS
TGTTTCAGATGCTGGTGCTG+GGG 0.605971 5.3:-5959883 None:intergenic
TGGGTTGGACCATTGATAGG+AGG 0.606483 5.3:+5959813 MS.gene025825:CDS
TTAGCCGCCGGTCCATTCAG+TGG 0.614841 5.3:+5959717 MS.gene025825:CDS
CTAATCCAAAAGTAACAGCT+GGG 0.617109 5.3:-5959094 None:intergenic
GCCGCCGGTCCATTCAGTGG+AGG 0.622801 5.3:+5959720 MS.gene025825:CDS
GGAGCTAACATCTTAGCCGC+CGG 0.625071 5.3:+5959705 MS.gene025825:CDS
GAGGAGGAGACATGCAACGA+TGG 0.629914 5.3:-5959180 None:intergenic
ACTCTGATAAATAAGCCTTG+AGG 0.644736 5.3:-5958046 None:intergenic
AGATGCAGCCTTGGATCCAG+CGG 0.648942 5.3:+5958986 MS.gene025825:CDS
ATAGCAGCAAACATCTCAGG+TGG 0.653320 5.3:+5959062 MS.gene025825:CDS
AGTTTCCACTAACCACAGCT+GGG 0.662001 5.3:-5959770 None:intergenic
GAGTGTTCCAACCCATGGAG+TGG 0.667041 5.3:+5959539 MS.gene025825:intron
TAGCGGTGACATAATTGAGG+AGG 0.669128 5.3:-5959196 None:intergenic
ACCTCCACTGAATGGACCGG+CGG 0.670839 5.3:-5959721 None:intergenic
TGATAAATAAGCCTTGAGGG+AGG 0.682523 5.3:-5958042 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATATATTAATATATTATGT+TGG + chr5.3:5959503-5959522 MS.gene025825:intron 10.0%
!! TTCAATGATTTATTAATATG+TGG - chr5.3:5959457-5959476 None:intergenic 15.0%
!!! AAAGTACATTACATAATTTT+AGG + chr5.3:5959230-5959249 MS.gene025825:intron 15.0%
!! AAAATTATGCAAGAAATAAG+AGG + chr5.3:5958205-5958224 MS.gene025825:intron 20.0%
!! AAATGTTTGACAAATGATAA+CGG + chr5.3:5958436-5958455 MS.gene025825:intron 20.0%
!! ACTACAATAAATTGCAATAT+TGG + chr5.3:5958538-5958557 MS.gene025825:intron 20.0%
!! ACTTCAATAAATTGCAATAT+TGG + chr5.3:5958907-5958926 MS.gene025825:intron 20.0%
!!! TCTATGAGATTTTCATAAAT+TGG - chr5.3:5958264-5958283 None:intergenic 20.0%
!!! TTTGAGATTATCATAACATT+TGG + chr5.3:5959594-5959613 MS.gene025825:CDS 20.0%
! AAATATAAGCTTACATCAGA+TGG + chr5.3:5958563-5958582 MS.gene025825:intron 25.0%
! GATTATCATAACATTTGGAT+TGG + chr5.3:5959599-5959618 MS.gene025825:CDS 25.0%
!! TACTTTTGAATATACCTTAG+CGG - chr5.3:5959216-5959235 None:intergenic 25.0%
!!! CATTACATAATTTTAGGTTC+AGG + chr5.3:5959236-5959255 MS.gene025825:intron 25.0%
AAATTGTTTCAGTCGAGATT+TGG - chr5.3:5959309-5959328 None:intergenic 30.0%
ACTATTGGTACAAATGTAGA+AGG + chr5.3:5958602-5958621 MS.gene025825:intron 30.0%
TGGTAACAATATTGGACTAT+TGG + chr5.3:5958587-5958606 MS.gene025825:intron 30.0%
TTACGTGAGACAAAATTTGA+AGG + chr5.3:5958484-5958503 MS.gene025825:intron 30.0%
! ATTTATGGTGACGTCTTTAT+TGG + chr5.3:5959852-5959871 MS.gene025825:CDS 30.0%
!! AATAAGCTTTGTAGTTCTAG+AGG + chr5.3:5958228-5958247 MS.gene025825:intron 30.0%
!!! GGTTGATGACAAATTGTTTT+GGG + chr5.3:5959273-5959292 MS.gene025825:intron 30.0%
!!! TACATAATTTTAGGTTCAGG+TGG + chr5.3:5959239-5959258 MS.gene025825:intron 30.0%
!!! TTTCATCCTCCAGTTTTAAA+TGG - chr5.3:5959387-5959406 None:intergenic 30.0%
ACTCTGATAAATAAGCCTTG+AGG - chr5.3:5958049-5958068 None:intergenic 35.0%
ATAAGCTTACATCAGATGGT+TGG + chr5.3:5958567-5958586 MS.gene025825:intron 35.0%
CAAACACTAATCCAGCAATA+GGG - chr5.3:5959578-5959597 None:intergenic 35.0%
CATGCATTTGCACTATTTGT+TGG + chr5.3:5959032-5959051 MS.gene025825:CDS 35.0%
CTAATCCAAAAGTAACAGCT+GGG - chr5.3:5959097-5959116 None:intergenic 35.0%
CTCTGATAAATAAGCCTTGA+GGG - chr5.3:5958048-5958067 None:intergenic 35.0%
GTAGAAGGAATGATGAAATG+TGG + chr5.3:5958617-5958636 MS.gene025825:intron 35.0%
TCAAACACTAATCCAGCAAT+AGG - chr5.3:5959579-5959598 None:intergenic 35.0%
TGCTGATAACTGGATCTATT+GGG + chr5.3:5959794-5959813 MS.gene025825:CDS 35.0%
TTAGTTGAACTCCTGAACAT+CGG + chr5.3:5959352-5959371 MS.gene025825:intron 35.0%
TTGCTGATAACTGGATCTAT+TGG + chr5.3:5959793-5959812 MS.gene025825:CDS 35.0%
! ATTTGCACTATTTGTTGGTG+TGG + chr5.3:5959037-5959056 MS.gene025825:CDS 35.0%
!! CAGATGGTTGGTAACAATAT+TGG + chr5.3:5958579-5958598 MS.gene025825:intron 35.0%
!!! CCTCCAGTTTTAAATGGAAA+GGG - chr5.3:5959381-5959400 None:intergenic 35.0%
!!! CGGTTGATGACAAATTGTTT+TGG + chr5.3:5959272-5959291 MS.gene025825:intron 35.0%
!!! CTCCAGTTTTAAATGGAAAG+GGG - chr5.3:5959380-5959399 None:intergenic 35.0%
!!! GTTACTTTTGGATTAGCCAT+TGG + chr5.3:5959101-5959120 MS.gene025825:CDS 35.0%
!!! TCCTCCAGTTTTAAATGGAA+AGG - chr5.3:5959382-5959401 None:intergenic 35.0%
!!! TTTTAGGTTCAGGTGGTTAA+TGG + chr5.3:5959246-5959265 MS.gene025825:intron 35.0%
ACAACGAACCCAATAGCAAT+CGG - chr5.3:5959684-5959703 None:intergenic 40.0%
AGACGTGACATAAATGAGGT+TGG - chr5.3:5958762-5958781 None:intergenic 40.0%
AGCGAACACAAAAATCAGAG+TGG - chr5.3:5958078-5958097 None:intergenic 40.0%
ATAGAAGAGACCAGTTAGGA+TGG - chr5.3:5959138-5959157 None:intergenic 40.0%
GATAACTGGATCTATTGGGT+TGG + chr5.3:5959798-5959817 MS.gene025825:CDS 40.0%
GCTAATCCAAAAGTAACAGC+TGG - chr5.3:5959098-5959117 None:intergenic 40.0%
GTGGAAACTTTGCTGATAAC+TGG + chr5.3:5959784-5959803 MS.gene025825:CDS 40.0%
GTTCAGGTGGTTAATGGTTT+CGG + chr5.3:5959252-5959271 MS.gene025825:intron 40.0%
TAGTTGAACTCCTGAACATC+GGG + chr5.3:5959353-5959372 MS.gene025825:intron 40.0%
TATTGGATTGCCCAATTGCT+TGG + chr5.3:5959155-5959174 MS.gene025825:CDS 40.0%
TCCAATAGAAGAGACCAGTT+AGG - chr5.3:5959142-5959161 None:intergenic 40.0%
TGATAAATAAGCCTTGAGGG+AGG - chr5.3:5958045-5958064 None:intergenic 40.0%
TTCATTGAACCTCCACTGAA+TGG - chr5.3:5959732-5959751 None:intergenic 40.0%
! ACTCTGATTTTTGTGTTCGC+TGG + chr5.3:5958077-5958096 MS.gene025825:CDS 40.0%
! ATTGCTATTGGGTTCGTTGT+CGG + chr5.3:5959684-5959703 MS.gene025825:CDS 40.0%
! ATTTTTGTGTTCGCTGGAGT+TGG + chr5.3:5958083-5958102 MS.gene025825:CDS 40.0%
! CCCTTTCCATTTAAAACTGG+AGG + chr5.3:5959378-5959397 MS.gene025825:intron 40.0%
! GACCCCTTTCCATTTAAAAC+TGG + chr5.3:5959375-5959394 MS.gene025825:intron 40.0%
! GTTCCAATTGAGCCCTTTTT+GGG - chr5.3:5959654-5959673 None:intergenic 40.0%
! TAAGGGTATGTTTCAGATGC+TGG - chr5.3:5959894-5959913 None:intergenic 40.0%
! TCCTAACTGGTCTCTTCTAT+TGG + chr5.3:5959138-5959157 MS.gene025825:CDS 40.0%
! TCTGAGACGTGACATAAATG+AGG - chr5.3:5958766-5958785 None:intergenic 40.0%
! TTCCAATTGAGCCCTTTTTG+GGG - chr5.3:5959653-5959672 None:intergenic 40.0%
!! TATTGGGTTGGACCATTGAT+AGG + chr5.3:5959810-5959829 MS.gene025825:CDS 40.0%
!!! ACTTTTGGATTAGCCATTGG+AGG + chr5.3:5959104-5959123 MS.gene025825:CDS 40.0%
!!! TTGAACCCAGCTGTTACTTT+TGG + chr5.3:5959089-5959108 MS.gene025825:CDS 40.0%
AACGATGGAGCCAAGCAATT+GGG - chr5.3:5959168-5959187 None:intergenic 45.0%
AAGTTTCCACTAACCACAGC+TGG - chr5.3:5959774-5959793 None:intergenic 45.0%
AGACGTGGCATAAATGAGGT+TGG - chr5.3:5958393-5958412 None:intergenic 45.0%
AGTTTCCACTAACCACAGCT+GGG - chr5.3:5959773-5959792 None:intergenic 45.0%
ATAGCAGCAAACATCTCAGG+TGG + chr5.3:5959062-5959081 MS.gene025825:CDS 45.0%
ATGTTTCAGATGCTGGTGCT+GGG - chr5.3:5959887-5959906 None:intergenic 45.0%
ATTTAGCATTGCCTCCCTCA+AGG + chr5.3:5958031-5958050 MS.gene025825:CDS 45.0%
CCTCAATTATGTCACCGCTA+AGG + chr5.3:5959199-5959218 MS.gene025825:CDS 45.0%
CCTTAGCGGTGACATAATTG+AGG - chr5.3:5959202-5959221 None:intergenic 45.0%
TAGCGGTGACATAATTGAGG+AGG - chr5.3:5959199-5959218 None:intergenic 45.0%
TATGTTTCAGATGCTGGTGC+TGG - chr5.3:5959888-5959907 None:intergenic 45.0%
TCTAACATCAGATGCAGCCT+TGG + chr5.3:5958977-5958996 MS.gene025825:CDS 45.0%
TTTCAGTCGAGATTTGGCCT+CGG - chr5.3:5959303-5959322 None:intergenic 45.0%
! AAACACACATGTCTGAGACG+TGG - chr5.3:5958408-5958427 None:intergenic 45.0%
! ACCTGAGATGTTTGCTGCTA+TGG - chr5.3:5959063-5959082 None:intergenic 45.0%
! GGTTCCAATTGAGCCCTTTT+TGG - chr5.3:5959655-5959674 None:intergenic 45.0%
! TCTGAGACGTGGCATAAATG+AGG - chr5.3:5958397-5958416 None:intergenic 45.0%
!! ACCATTGCACCGATTGCTAT+TGG + chr5.3:5959672-5959691 MS.gene025825:CDS 45.0%
!! CCATTGCACCGATTGCTATT+GGG + chr5.3:5959673-5959692 MS.gene025825:CDS 45.0%
!!! AAATTGTTTTGGGAAGCCCG+AGG + chr5.3:5959283-5959302 MS.gene025825:intron 45.0%
ACAGCAGCTGACCCCAAAAA+GGG + chr5.3:5959639-5959658 MS.gene025825:CDS 50.0%
ACCATTGATAGGAGGAGGCT+TGG + chr5.3:5959821-5959840 MS.gene025825:CDS 50.0%
CAACGATGGAGCCAAGCAAT+TGG - chr5.3:5959169-5959188 None:intergenic 50.0%
CAGTGGAGGTTCAATGAACC+CGG + chr5.3:5959734-5959753 MS.gene025825:CDS 50.0%
CCCAATAGCAATCGGTGCAA+TGG - chr5.3:5959676-5959695 None:intergenic 50.0%
GACCCCAAAAAGGGCTCAAT+TGG + chr5.3:5959648-5959667 MS.gene025825:CDS 50.0%
GCCATAGCAGCAAACATCTC+AGG + chr5.3:5959059-5959078 MS.gene025825:CDS 50.0%
GGCAACATCACCATCCTAAC+TGG + chr5.3:5959125-5959144 MS.gene025825:CDS 50.0%
GTTGGACCATTGATAGGAGG+AGG + chr5.3:5959816-5959835 MS.gene025825:CDS 50.0%
TGAACCTCCACTGAATGGAC+CGG - chr5.3:5959727-5959746 None:intergenic 50.0%
TTCAGTCGAGATTTGGCCTC+GGG - chr5.3:5959302-5959321 None:intergenic 50.0%
! GATGGTGATGTTGCCTCCAA+TGG - chr5.3:5959120-5959139 None:intergenic 50.0%
! TGGGTTGGACCATTGATAGG+AGG + chr5.3:5959813-5959832 MS.gene025825:CDS 50.0%
! TGTTTCAGATGCTGGTGCTG+GGG - chr5.3:5959886-5959905 None:intergenic 50.0%
! TTGCAGAGTGTTCCAACCCA+TGG + chr5.3:5959534-5959553 MS.gene025825:intron 50.0%
!! GGCTTGGCTGGGTTGATTTA+TGG + chr5.3:5959837-5959856 MS.gene025825:CDS 50.0%
AGATGCAGCCTTGGATCCAG+CGG + chr5.3:5958986-5959005 MS.gene025825:CDS 55.0%
CACAGCAGCTGACCCCAAAA+AGG + chr5.3:5959638-5959657 MS.gene025825:CDS 55.0%
CACTAAACCCGCTGGATCCA+AGG - chr5.3:5958997-5959016 None:intergenic 55.0%
GAGGAGGAGACATGCAACGA+TGG - chr5.3:5959183-5959202 None:intergenic 55.0%
GAGTGTTCCAACCCATGGAG+TGG + chr5.3:5959539-5959558 MS.gene025825:intron 55.0%
GCCAAGCCTCCTCCTATCAA+TGG - chr5.3:5959825-5959844 None:intergenic 55.0%
GCTGGACTGAACCCTATTGC+TGG + chr5.3:5959564-5959583 MS.gene025825:CDS 55.0%
GGAGCTAACATCTTAGCCGC+CGG + chr5.3:5959705-5959724 MS.gene025825:CDS 55.0%
GGGTTTAGTGGCAGTAGCTG+TGG + chr5.3:5959007-5959026 MS.gene025825:CDS 55.0%
TGATAGGAGGAGGCTTGGCT+GGG + chr5.3:5959826-5959845 MS.gene025825:CDS 55.0%
TTGATAGGAGGAGGCTTGGC+TGG + chr5.3:5959825-5959844 MS.gene025825:CDS 55.0%
! ATGAACCCGGCTCGCTCATT+CGG + chr5.3:5959747-5959766 MS.gene025825:CDS 55.0%
! CTTGGATCCAGCGGGTTTAG+TGG + chr5.3:5958995-5959014 MS.gene025825:CDS 55.0%
!!! CTTTTTGGGGTCAGCTGCTG+TGG - chr5.3:5959640-5959659 None:intergenic 55.0%
ACCTCCACTGAATGGACCGG+CGG - chr5.3:5959724-5959743 None:intergenic 60.0%
CAGTCCAGCAGCCACTCCAT+GGG - chr5.3:5959553-5959572 None:intergenic 60.0%
GATGCAGCCTTGGATCCAGC+GGG + chr5.3:5958987-5959006 MS.gene025825:CDS 60.0%
GCTACTGCCACTAAACCCGC+TGG - chr5.3:5959005-5959024 None:intergenic 60.0%
GGAAAGGGGTCCCGATGTTC+AGG - chr5.3:5959366-5959385 None:intergenic 60.0%
TCAGTCCAGCAGCCACTCCA+TGG - chr5.3:5959554-5959573 None:intergenic 60.0%
TTAGCCGCCGGTCCATTCAG+TGG + chr5.3:5959717-5959736 MS.gene025825:CDS 60.0%
TTCGGCCCAGCTGTGGTTAG+TGG + chr5.3:5959765-5959784 MS.gene025825:CDS 60.0%
CCAACCCATGGAGTGGCTGC+TGG + chr5.3:5959546-5959565 MS.gene025825:CDS 65.0%
CCAGCAGCCACTCCATGGGT+TGG - chr5.3:5959549-5959568 None:intergenic 65.0%
TCGCTCATTCGGCCCAGCTG+TGG + chr5.3:5959758-5959777 MS.gene025825:CDS 65.0%
CTGGGCCGAATGAGCGAGCC+GGG - chr5.3:5959755-5959774 None:intergenic 70.0%
GCCGCCGGTCCATTCAGTGG+AGG + chr5.3:5959720-5959739 MS.gene025825:CDS 70.0%
GCTGGGCCGAATGAGCGAGC+CGG - chr5.3:5959756-5959775 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 5957996 5959914 5957996 ID=MS.gene025825
chr5.3 mRNA 5957996 5959914 5957996 ID=MS.gene025825.t1;Parent=MS.gene025825
chr5.3 exon 5957996 5958122 5957996 ID=MS.gene025825.t1.exon1;Parent=MS.gene025825.t1
chr5.3 CDS 5957996 5958122 5957996 ID=cds.MS.gene025825.t1;Parent=MS.gene025825.t1
chr5.3 exon 5958973 5959220 5958973 ID=MS.gene025825.t1.exon2;Parent=MS.gene025825.t1
chr5.3 CDS 5958973 5959220 5958973 ID=cds.MS.gene025825.t1;Parent=MS.gene025825.t1
chr5.3 exon 5959540 5959914 5959540 ID=MS.gene025825.t1.exon3;Parent=MS.gene025825.t1
chr5.3 CDS 5959540 5959914 5959540 ID=cds.MS.gene025825.t1;Parent=MS.gene025825.t1
Gene Sequence

>MS.gene025825

ATGGTGAAGATAGGTTTTGGTACATTTGATGACTCATTTAGCATTGCCTCCCTCAAGGCTTATTTATCAGAGTTCATTGCCACTCTGATTTTTGTGTTCGCTGGAGTTGGATCAGCAATTGCTTACAGTAAGTAGTGTTCATGTGTAACTAATTTTTTTTTTTTTTTTATCTTTTTAAAGAAGTGTGACTAGTTATATGATTTGAAACAAAAATTATGCAAGAAATAAGAGGAATAAGCTTTGTAGTTCTAGAGGTTTTTGTCGTCCAATTTATGAAAATCTCATAGACTTCTTCTCTTGTATAATATTTGTCTCAAATCGTTTAAGTTAATTTTAAGATAGACAAGAGTTACATGTAGAAGATGAAAAATGAATACTTGCGTATGATAAGTGACCAACCTCATTTATGCCACGTCTCAGACATGTGTGTTTTTGTCACGAAATGTTTGACAAATGATAACGGTGTCATCTAGTAAGACTTAAAAGATTTACGTGAGACAAAATTTGAAGGAATATTTTACTTTTGTTAAGAGTTATTAGCAACTACAATAAATTGCAATATTGGCAAAATATAAGCTTACATCAGATGGTTGGTAACAATATTGGACTATTGGTACAAATGTAGAAGGAATGATGAAATGTGGTTGTTTTGCATTATGCAGATGATCTAACATCATTTGTCTCAAATCGTTTAAGTTAATTTTAAGATAGACAAGAGTTACATGTAGAAGATGAAAAATGAATACGTGTGTATGATAAGTGACCAACCTCATTTATGTCACGTCTCAGACATGTGTGTTTTTGTCACGAAATGTTTGACAAATGATAACGGTGTCATCTAGTAAGACTTAAAAGATTTACTTGAGACAAAATTTGAAAGAATATTTTACTTTTGTTAAGAGTTATTAGCAACTTCAATAAATTGCAATATTGGCAAAATATAAGCTTACATCAGATGGTTGTTTTGCATTATGCAGATGATCTAACATCAGATGCAGCCTTGGATCCAGCGGGTTTAGTGGCAGTAGCTGTGGCTCATGCATTTGCACTATTTGTTGGTGTGGCCATAGCAGCAAACATCTCAGGTGGACATTTGAACCCAGCTGTTACTTTTGGATTAGCCATTGGAGGCAACATCACCATCCTAACTGGTCTCTTCTATTGGATTGCCCAATTGCTTGGCTCCATCGTTGCATGTCTCCTCCTCAATTATGTCACCGCTAAGGTATATTCAAAAGTACATTACATAATTTTAGGTTCAGGTGGTTAATGGTTTCGGTTGATGACAAATTGTTTTGGGAAGCCCGAGGCCAAATCTCGACTGAAACAATTTTTTGTTTAAATGTGTCTTATCTCTTAGTTGAACTCCTGAACATCGGGACCCCTTTCCATTTAAAACTGGAGGATGAAAAAAAGTTACACATTAACGACTTCTTTTACTCATAATCATAACAAGTTCCACATATTAATAAATCATTGAATAGTTGATAAATATACATTAAATTTTCATATATTAATATATTATGTTGGATATGTATTTGCAGAGTGTTCCAACCCATGGAGTGGCTGCTGGACTGAACCCTATTGCTGGATTAGTGTTTGAGATTATCATAACATTTGGATTGGTTTACACTGTTTATGCCACAGCAGCTGACCCCAAAAAGGGCTCAATTGGAACCATTGCACCGATTGCTATTGGGTTCGTTGTCGGAGCTAACATCTTAGCCGCCGGTCCATTCAGTGGAGGTTCAATGAACCCGGCTCGCTCATTCGGCCCAGCTGTGGTTAGTGGAAACTTTGCTGATAACTGGATCTATTGGGTTGGACCATTGATAGGAGGAGGCTTGGCTGGGTTGATTTATGGTGACGTCTTTATTGGTTCATATTCCCCAGCACCAGCATCTGAAACATACCCTTAA
Protein sequence

>MS.gene025825.t1

MVKIGFGTFDDSFSIASLKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGSIVACLLLNYVTAKSVPTHGVAAGLNPIAGLVFEIIITFGLVYTVYATAADPKKGSIGTIAPIAIGFVVGANILAAGPFSGGSMNPARSFGPAVVSGNFADNWIYWVGPLIGGGLAGLIYGDVFIGSYSPAPASETYP