AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85141

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85141.t1 MTR_2g101370 97.992 249 5 0 1 249 1 249 5.33e-171 471
MS.gene85141.t1 MTR_8g013680 86.585 246 33 0 4 249 3 248 9.14e-148 412
MS.gene85141.t1 MTR_5g012810 76.327 245 57 1 5 249 4 247 2.70e-114 328
MS.gene85141.t1 MTR_4g063090 69.369 222 67 1 25 245 26 247 2.89e-98 287
MS.gene85141.t1 MTR_1g115405 58.400 250 100 3 3 249 4 252 1.27e-90 268
MS.gene85141.t1 MTR_1g077540 52.400 250 112 1 6 248 7 256 6.25e-89 264
MS.gene85141.t1 MTR_7g023350 61.044 249 95 2 3 249 4 252 1.45e-88 263
MS.gene85141.t1 MTR_1g006490 57.377 244 100 3 5 248 4 243 2.03e-88 262
MS.gene85141.t1 MTR_7g103030 61.633 245 93 1 3 247 4 247 2.26e-87 259
MS.gene85141.t1 MTR_6g069040 55.870 247 105 1 6 248 7 253 2.78e-84 252
MS.gene85141.t1 MTR_1g006590 55.510 245 103 4 5 248 4 243 5.35e-83 248
MS.gene85141.t1 MTR_2g060360 46.581 234 124 1 9 242 8 240 1.28e-70 217
MS.gene85141.t1 MTR_5g063930 37.860 243 139 4 10 249 27 260 4.48e-40 139
MS.gene85141.t1 MTR_2g094270 37.247 247 138 6 10 240 30 275 5.21e-40 139
MS.gene85141.t1 MTR_7g101190 37.712 236 131 5 17 237 36 270 4.52e-38 134
MS.gene85141.t1 MTR_3g118010 38.723 235 124 6 17 237 34 262 5.72e-38 134
MS.gene85141.t1 MTR_4g059390 38.017 242 128 7 17 240 36 273 9.41e-37 131
MS.gene85141.t1 MTR_2g104940 39.035 228 119 8 20 241 80 293 8.93e-33 121
MS.gene85141.t1 MTR_4g006730 36.681 229 125 6 20 242 75 289 1.97e-32 120
MS.gene85141.t1 MTR_1g097840 39.640 222 113 7 15 230 69 275 2.65e-32 120
MS.gene85141.t1 MTR_5g010150 35.897 234 135 5 15 236 47 277 1.96e-31 117
MS.gene85141.t1 MTR_2g094270 37.445 227 116 7 10 219 30 247 2.73e-31 116
MS.gene85141.t1 MTR_8g098375 35.470 234 136 5 15 236 47 277 3.62e-31 116
MS.gene85141.t1 MTR_5g082070 36.364 231 132 5 15 233 50 277 6.65e-31 116
MS.gene85141.t1 MTR_3g070210 34.615 234 138 5 15 236 50 280 6.35e-30 113
MS.gene85141.t1 MTR_4g006630 36.111 216 118 6 33 242 1 202 1.43e-29 110
MS.gene85141.t1 MTR_4g006730 36.111 216 118 6 33 242 1 202 1.91e-29 110
MS.gene85141.t1 MTR_4g415300 34.764 233 138 5 15 236 51 280 9.78e-29 110
MS.gene85141.t1 MTR_3g008920 38.750 240 121 10 16 247 22 243 1.29e-28 109
MS.gene85141.t1 MTR_5g079570 38.075 239 120 10 15 244 35 254 3.85e-28 108
MS.gene85141.t1 MTR_1g095070 38.919 185 93 6 68 237 25 204 6.89e-28 106
MS.gene85141.t1 MTR_2g017620 33.898 236 141 6 11 243 17 240 1.76e-27 106
MS.gene85141.t1 MTR_2g017620 33.772 228 138 5 19 243 37 254 2.69e-27 106
MS.gene85141.t1 MTR_2g017590 33.468 248 147 8 6 247 19 254 3.46e-27 105
MS.gene85141.t1 MTR_1g070385 34.404 218 124 6 20 232 62 265 2.85e-26 107
MS.gene85141.t1 MTR_8g087710 33.058 242 144 7 15 249 34 264 3.74e-25 100
MS.gene85141.t1 MTR_8g087720 32.129 249 154 6 8 249 23 263 4.34e-25 100
MS.gene85141.t1 MTR_7g010650 34.234 222 131 5 24 242 41 250 7.53e-25 99.8
MS.gene85141.t1 MTR_4g084880 32.780 241 146 6 16 249 31 262 9.10e-25 99.8
MS.gene85141.t1 MTR_2g017660 32.340 235 150 5 15 249 34 259 2.63e-24 98.6
MS.gene85141.t1 MTR_2g017620 37.059 170 99 3 77 243 9 173 7.43e-24 95.1
MS.gene85141.t1 MTR_2g017610 31.004 229 149 5 19 247 37 256 3.82e-23 95.1
MS.gene85141.t1 MTR_3g070210 34.483 203 121 4 15 208 50 249 1.12e-22 94.0
MS.gene85141.t1 MTR_2g017570 30.568 229 150 5 19 247 37 256 1.21e-22 94.0
MS.gene85141.t1 MTR_4g059390 37.059 170 92 5 20 178 39 204 5.31e-21 87.8
MS.gene85141.t1 MTR_1g095070 41.176 153 73 5 68 208 25 172 6.69e-21 87.4
MS.gene85141.t1 MTR_5g010150 34.320 169 103 3 15 178 47 212 4.85e-18 80.5
MS.gene85141.t1 MTR_4g006730 35.795 176 95 4 20 190 75 237 8.74e-18 80.5
MS.gene85141.t1 MTR_8g098375 32.558 172 108 3 15 181 47 215 1.58e-17 79.3
MS.gene85141.t1 MTR_4g415300 33.516 182 112 4 15 192 51 227 6.39e-17 77.4
MS.gene85141.t1 MTR_3g070210 32.738 168 105 3 15 177 50 214 1.77e-16 75.9
MS.gene85141.t1 MTR_1g097840 35.165 182 98 6 15 190 69 236 4.70e-15 73.2
MS.gene85141.t1 MTR_8g468730 40.187 107 42 3 136 242 2 86 1.40e-14 68.2
MS.gene85141.t1 MTR_4g075570 28.279 244 142 7 8 224 33 270 2.02e-14 72.0
MS.gene85141.t1 MTR_4g006650 34.459 148 72 4 24 166 8 135 2.73e-13 68.6
MS.gene85141.t1 MTR_4g075590 25.207 242 152 6 12 233 29 261 8.38e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85141.t1 AT3G16240 82.258 248 42 2 4 249 3 250 6.38e-142 398
MS.gene85141.t1 AT5G47450 75.847 236 57 0 5 240 4 239 1.02e-122 349
MS.gene85141.t1 AT4G17340 76.695 236 55 0 5 240 4 239 4.13e-114 327
MS.gene85141.t1 AT2G36830 65.306 245 85 0 3 247 4 248 1.71e-104 303
MS.gene85141.t1 AT3G26520 67.094 234 77 0 8 241 10 243 1.78e-103 301
MS.gene85141.t1 AT4G01470 60.729 247 96 1 3 248 4 250 5.22e-94 276
MS.gene85141.t1 AT1G73190 56.250 256 98 2 7 248 11 266 3.54e-85 254
MS.gene85141.t1 AT1G17810 56.471 255 98 2 7 248 11 265 9.08e-82 246
MS.gene85141.t1 AT2G25810 54.918 244 106 2 5 248 4 243 5.40e-80 241
MS.gene85141.t1 AT3G47440 44.444 234 129 1 9 242 12 244 6.05e-66 205
MS.gene85141.t1 AT4G35100 38.333 240 128 6 15 240 33 266 3.51e-37 132
MS.gene85141.t1 AT4G35100 38.333 240 128 6 15 240 33 266 3.51e-37 132
MS.gene85141.t1 AT2G37170 37.354 257 135 8 11 247 28 278 4.89e-37 132
MS.gene85141.t1 AT2G37170 37.354 257 135 8 11 247 71 321 8.83e-37 132
MS.gene85141.t1 AT3G54820 37.607 234 128 7 20 237 38 269 2.41e-36 130
MS.gene85141.t1 AT3G53420 38.525 244 132 7 20 247 39 280 3.21e-36 130
MS.gene85141.t1 AT3G53420 38.525 244 132 7 20 247 39 280 3.21e-36 130
MS.gene85141.t1 AT5G60660 37.339 233 130 7 20 237 39 270 5.98e-36 129
MS.gene85141.t1 AT2G37180 36.842 247 138 6 17 247 34 278 8.15e-36 129
MS.gene85141.t1 AT2G39010 37.131 237 125 7 20 237 38 269 5.88e-35 127
MS.gene85141.t1 AT2G16850 37.860 243 129 7 20 247 36 271 1.59e-34 125
MS.gene85141.t1 AT4G10380 36.100 241 130 5 4 233 56 283 2.83e-33 122
MS.gene85141.t1 AT1G52180 71.429 84 24 0 129 212 38 121 1.28e-32 115
MS.gene85141.t1 AT1G80760 35.776 232 131 5 15 241 75 293 2.47e-32 120
MS.gene85141.t1 AT4G00430 36.864 236 129 6 15 236 48 277 4.68e-31 116
MS.gene85141.t1 AT4G23400 36.481 233 134 5 15 236 48 277 1.49e-30 115
MS.gene85141.t1 AT1G01620 36.441 236 130 6 15 236 47 276 1.80e-30 115
MS.gene85141.t1 AT3G61430 36.052 233 129 6 15 233 47 273 1.58e-29 112
MS.gene85141.t1 AT3G61430 36.052 233 129 6 15 233 47 273 1.58e-29 112
MS.gene85141.t1 AT2G45960 35.193 233 131 6 15 233 47 273 8.70e-29 110
MS.gene85141.t1 AT5G37820 34.375 224 138 4 24 247 47 261 2.35e-27 106
MS.gene85141.t1 AT5G37820 34.375 224 138 4 24 247 83 297 2.42e-27 107
MS.gene85141.t1 AT1G01620 38.265 196 101 6 56 236 14 204 6.95e-27 103
MS.gene85141.t1 AT5G37810 34.272 213 133 3 24 236 47 252 4.01e-25 100
MS.gene85141.t1 AT4G19030 33.191 235 135 5 13 236 48 271 1.24e-24 99.8
MS.gene85141.t1 AT4G18910 33.190 232 139 5 13 236 45 268 2.79e-24 98.6
MS.gene85141.t1 AT3G06100 32.877 219 140 4 7 225 33 244 6.59e-24 97.4
MS.gene85141.t1 AT1G31885 34.199 231 130 5 15 234 38 257 1.14e-23 97.8
MS.gene85141.t1 AT3G06100 34.158 202 126 4 24 225 2 196 3.08e-22 91.7
MS.gene85141.t1 AT2G45960 35.096 208 117 6 20 216 52 252 7.60e-22 91.7
MS.gene85141.t1 AT2G45960 35.211 213 120 6 15 216 47 252 1.25e-21 91.7
MS.gene85141.t1 AT1G31885 35.789 190 111 3 53 234 17 203 5.45e-21 89.4
MS.gene85141.t1 AT2G39010 36.471 170 91 5 20 177 38 202 2.80e-20 86.7
MS.gene85141.t1 AT4G00430 35.429 175 100 4 15 182 48 216 2.51e-17 78.6
MS.gene85141.t1 AT2G34390 30.769 234 145 6 14 240 42 265 9.35e-16 75.5
MS.gene85141.t1 AT2G34390 31.250 224 137 6 24 240 10 223 5.01e-15 72.8
MS.gene85141.t1 AT3G06100 35.772 123 77 2 104 226 44 164 3.68e-14 69.3

Find 79 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGACCTTCGGGTTGGCTAT+TGG 0.189119 2.4:-3382198 MS.gene85141:CDS
TATTGGGTTGGACCTCTTAT+TGG 0.194787 2.4:-3381707 MS.gene85141:CDS
TGACCTTCGGGTTGGCTATT+GGG 0.195713 2.4:-3382197 MS.gene85141:CDS
TACTGGATTGCACAACTTCT+TGG 0.270070 2.4:-3382144 MS.gene85141:CDS
GTCCCTAATGATCCTTTCTT+AGG 0.271255 2.4:+3381866 None:intergenic
ACCATAACAACTGGATCTAT+TGG 0.279624 2.4:-3381724 MS.gene85141:CDS
CTTTCAGTGTGAGCTCCATT+AGG 0.280151 2.4:-3382634 MS.gene85141:CDS
CCTATCCACAGTGTTGCTTC+TGG 0.305795 2.4:-3381971 MS.gene85141:CDS
ATGAATCCGGCACGTTCATT+TGG 0.314171 2.4:-3381770 MS.gene85141:CDS
ACAGAAATTATCATAACATT+TGG 0.330200 2.4:-3381923 MS.gene85141:CDS
GATCCTAAGAAAGGATCATT+AGG 0.331020 2.4:-3381869 MS.gene85141:CDS
ACCTCTTATTGGTGGTGGTT+TGG 0.336316 2.4:-3381696 MS.gene85141:CDS
GGTTTGGCTGGTGTCATCTA+TGG 0.353100 2.4:-3381680 MS.gene85141:CDS
GTCAACCCAGCCGTGACCTT+CGG 0.360207 2.4:-3382210 MS.gene85141:CDS
TTTCAGTGTGAGCTCCATTA+GGG 0.361180 2.4:-3382633 MS.gene85141:CDS
CCCAATAGATCCAGTTGTTA+TGG 0.363853 2.4:+3381723 None:intergenic
CTCAGCAGCATAAGCCCTAA+TGG 0.364884 2.4:+3382619 None:intergenic
ACAATTGCACCAATTGCTAT+TGG 0.368535 2.4:-3381845 MS.gene85141:CDS
CTTATTGGTGGTGGTTTGGC+TGG 0.371194 2.4:-3381692 MS.gene85141:CDS
TCAACCCAGCCGTGACCTTC+GGG 0.382552 2.4:-3382209 MS.gene85141:CDS
ATTGCTATTGGTTTGATTGT+TGG 0.383502 2.4:-3381833 MS.gene85141:CDS
TCTGTTGGAGCCAACATATC+TGG 0.390137 2.4:-3382240 MS.gene85141:CDS
ATGGGTCATGGCATAGCATT+TGG 0.391911 2.4:-3382671 MS.gene85141:CDS
GTTGCTTCTGGTGTTGGAGC+TGG 0.401927 2.4:-3381959 MS.gene85141:CDS
TGAATCCGGCACGTTCATTT+GGG 0.407305 2.4:-3381769 MS.gene85141:CDS
CACAGTGTTGCTTCTGGTGT+TGG 0.413388 2.4:-3381965 MS.gene85141:CDS
GCCCTCAGACCATGTACCTT+TGG 0.420648 2.4:-3381645 MS.gene85141:CDS
CCATAACAACTGGATCTATT+GGG 0.423483 2.4:-3381723 MS.gene85141:CDS
CTCTGGTGGTTCAATGAATC+CGG 0.425020 2.4:-3381783 MS.gene85141:CDS
GACCTTCGGGTTGGCTATTG+GGG 0.433165 2.4:-3382196 MS.gene85141:CDS
CTCCTCAAGTTTGCCACAGG+AGG 0.433870 2.4:-3382102 MS.gene85141:CDS
TCCTCACCGGTATCTTCTAC+TGG 0.444081 2.4:-3382161 MS.gene85141:CDS
GTGGTGACTACCATAACAAC+TGG 0.450298 2.4:-3381733 MS.gene85141:CDS
GGTGCAGCTCTTGATCCTGC+TGG 0.451296 2.4:-3382312 MS.gene85141:CDS
TGGGTTGGACCTCTTATTGG+TGG 0.464450 2.4:-3381704 MS.gene85141:CDS
AATTATCATAACATTTGGAT+TGG 0.465289 2.4:-3381918 MS.gene85141:CDS
TTTGCAGCTAAGTTGACATC+AGG 0.466178 2.4:-3382333 MS.gene85141:intron
GTTGGACCTCTTATTGGTGG+TGG 0.469086 2.4:-3381701 MS.gene85141:CDS
ACAATCAAACCAATAGCAAT+TGG 0.470369 2.4:+3381836 None:intergenic
GAGGAGAAAGCATGCCACTA+TGG 0.473275 2.4:+3382119 None:intergenic
CAAGTTTGCCACAGGAGGCT+TGG 0.473516 2.4:-3382097 MS.gene85141:intron
TTAGCTGCAGGACCATTCTC+TGG 0.477678 2.4:-3381800 MS.gene85141:CDS
GCCAACCCGAAGGTCACGGC+TGG 0.481029 2.4:+3382204 None:intergenic
GCCAAACCACCACCAATAAG+AGG 0.493921 2.4:+3381695 None:intergenic
ACAACTTCTTGGTTCCATAG+TGG 0.497833 2.4:-3382133 MS.gene85141:CDS
GTTGGTTCAGCCATAGCCTA+TGG 0.499410 2.4:-3382563 MS.gene85141:intron
TCTGGTGTTGGAGCTGGTGA+AGG 0.508041 2.4:-3381953 MS.gene85141:CDS
TTTCTCCTCAAGTTTGCCAC+AGG 0.511598 2.4:-3382105 MS.gene85141:CDS
ATCCTAAGAAAGGATCATTA+GGG 0.512697 2.4:-3381868 MS.gene85141:CDS
TTCATTGAACCACCAGAGAA+TGG 0.521007 2.4:+3381788 None:intergenic
TTTGGGCCTGCAGTTGTAAG+TGG 0.525678 2.4:-3381752 MS.gene85141:CDS
AAGAGCAAAACCATGACAAA+CGG 0.527064 2.4:+3382275 None:intergenic
AACAACTGGATCTATTGGGT+TGG 0.532605 2.4:-3381719 MS.gene85141:CDS
GGTGCCAACATCTTAGCTGC+AGG 0.533016 2.4:-3381812 MS.gene85141:CDS
GTAGCAGTTGCCGTTTGTCA+TGG 0.534024 2.4:-3382285 MS.gene85141:CDS
AATCACTAGCCAAAGGTACA+TGG 0.550844 2.4:+3381636 None:intergenic
GACATGTCCACCAGATATGT+TGG 0.557831 2.4:+3382230 None:intergenic
CCCAGCCGTGACCTTCGGGT+TGG 0.560693 2.4:-3382205 MS.gene85141:CDS
TTTCTTAGGATCAGCTGCTG+TGG 0.561728 2.4:+3381880 None:intergenic
AGCCTCCTGTGGCAAACTTG+AGG 0.564648 2.4:+3382100 None:intergenic
ACAGCAGCTGATCCTAAGAA+AGG 0.565051 2.4:-3381878 MS.gene85141:CDS
ACCTTCGGGTTGGCTATTGG+GGG 0.570113 2.4:-3382195 MS.gene85141:CDS
AAACTTGTGTAGATGGGTCA+TGG 0.577221 2.4:-3382683 None:intergenic
TGCAATCCAGTAGAAGATAC+CGG 0.579827 2.4:+3382155 None:intergenic
TAGTCACCACTTACAACTGC+AGG 0.580347 2.4:+3381746 None:intergenic
GCAACTGCTACTAATCCAGC+AGG 0.581333 2.4:+3382297 None:intergenic
AATAGCCAACCCGAAGGTCA+CGG 0.584161 2.4:+3382200 None:intergenic
AGCCAAAGGTACATGGTCTG+AGG 0.586441 2.4:+3381643 None:intergenic
GCCAAAGGTACATGGTCTGA+GGG 0.595712 2.4:+3381644 None:intergenic
GCAGGCCCAAATGAACGTGC+CGG 0.597880 2.4:+3381764 None:intergenic
GCTGCAGGACCATTCTCTGG+TGG 0.598201 2.4:-3381797 MS.gene85141:CDS
CCAACCCGAAGGTCACGGCT+GGG 0.609276 2.4:+3382205 None:intergenic
CCAGAAGCAACACTGTGGAT+AGG 0.616969 2.4:+3381971 None:intergenic
TGGTCCTGCAGCTAAGATGT+TGG 0.619690 2.4:+3381808 None:intergenic
GTTGGAGCCAACATATCTGG+TGG 0.627384 2.4:-3382237 MS.gene85141:CDS
GGACAGATCACTATCCTCAC+CGG 0.633976 2.4:-3382174 MS.gene85141:CDS
TAAACATACCAAGCCTCCTG+TGG 0.686094 2.4:+3382089 None:intergenic
TCCAGTAGAAGATACCGGTG+AGG 0.726773 2.4:+3382160 None:intergenic
CAACACCAGAAGCAACACTG+TGG 0.753730 2.4:+3381966 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCAATGAAATATTTTATCA+AGG + chr2.4:3381850-3381869 None:intergenic 15.0%
!! AATTATCATAACATTTGGAT+TGG - chr2.4:3382384-3382403 MS.gene85141:intron 20.0%
!! ACAGAAATTATCATAACATT+TGG - chr2.4:3382379-3382398 MS.gene85141:intron 20.0%
!!! TGATGTTTTAAAATTCGAAA+AGG + chr2.4:3382264-3382283 None:intergenic 20.0%
! AAATAACAAAGTTGGAGTTA+TGG - chr2.4:3381919-3381938 MS.gene85141:CDS 25.0%
! AAGAAGAAAAAACTAACCAT+AGG + chr2.4:3381758-3381777 None:intergenic 25.0%
! TATGTTGCAAATAACAAAGT+TGG - chr2.4:3381911-3381930 MS.gene85141:CDS 25.0%
!! GTATTACTTTAGAAGTTTCA+TGG + chr2.4:3381817-3381836 None:intergenic 25.0%
!!! GTACAAAGTTTTTAAGTGTT+TGG + chr2.4:3381948-3381967 None:intergenic 25.0%
AAAAAACTAACCATAGGCTA+TGG + chr2.4:3381752-3381771 None:intergenic 30.0%
ACAATCAAACCAATAGCAAT+TGG + chr2.4:3382469-3382488 None:intergenic 30.0%
ACATAAGATACAGTCAGTTA+TGG + chr2.4:3382293-3382312 None:intergenic 30.0%
ATCCTAAGAAAGGATCATTA+GGG - chr2.4:3382434-3382453 MS.gene85141:intron 30.0%
GGTCATGTATAATCTTGATT+TGG + chr2.4:3381796-3381815 None:intergenic 30.0%
!! ATTGCTATTGGTTTGATTGT+TGG - chr2.4:3382469-3382488 MS.gene85141:intron 30.0%
AAGAGCAAAACCATGACAAA+CGG + chr2.4:3382030-3382049 None:intergenic 35.0%
ACAATTGCACCAATTGCTAT+TGG - chr2.4:3382457-3382476 MS.gene85141:intron 35.0%
ACCAGCAAAAACAAAGATCA+AGG + chr2.4:3381719-3381738 None:intergenic 35.0%
ACCATAACAACTGGATCTAT+TGG - chr2.4:3382578-3382597 MS.gene85141:CDS 35.0%
CCATAACAACTGGATCTATT+GGG - chr2.4:3382579-3382598 MS.gene85141:CDS 35.0%
GATCCTAAGAAAGGATCATT+AGG - chr2.4:3382433-3382452 MS.gene85141:intron 35.0%
!!! ACCTTGATCTTTGTTTTTGC+TGG - chr2.4:3381715-3381734 MS.gene85141:CDS 35.0%
!!! ATCTTTGTTTTTGCTGGTGT+TGG - chr2.4:3381721-3381740 MS.gene85141:CDS 35.0%
!!! CTTTTTGTTGCTGTTTCTGT+TGG - chr2.4:3382047-3382066 MS.gene85141:intron 35.0%
AACAACTGGATCTATTGGGT+TGG - chr2.4:3382583-3382602 MS.gene85141:CDS 40.0%
ACAACTTCTTGGTTCCATAG+TGG - chr2.4:3382169-3382188 MS.gene85141:CDS 40.0%
CCCAATAGATCCAGTTGTTA+TGG + chr2.4:3382582-3382601 None:intergenic 40.0%
GTCCCTAATGATCCTTTCTT+AGG + chr2.4:3382439-3382458 None:intergenic 40.0%
TACTGGATTGCACAACTTCT+TGG - chr2.4:3382158-3382177 MS.gene85141:CDS 40.0%
TGCAATCCAGTAGAAGATAC+CGG + chr2.4:3382150-3382169 None:intergenic 40.0%
TTCATTGAACCACCAGAGAA+TGG + chr2.4:3382517-3382536 None:intergenic 40.0%
TTTCAGTGTGAGCTCCATTA+GGG - chr2.4:3381669-3381688 MS.gene85141:CDS 40.0%
! AATCACTAGCCAAAGGTACA+TGG + chr2.4:3382669-3382688 None:intergenic 40.0%
! TTTGCAGCTAAGTTGACATC+AGG - chr2.4:3381969-3381988 MS.gene85141:CDS 40.0%
!! TATTGGGTTGGACCTCTTAT+TGG - chr2.4:3382595-3382614 MS.gene85141:CDS 40.0%
ACAGCAGCTGATCCTAAGAA+AGG - chr2.4:3382424-3382443 MS.gene85141:intron 45.0%
ATGAATCCGGCACGTTCATT+TGG - chr2.4:3382532-3382551 MS.gene85141:intron 45.0%
ATGGGTCATGGCATAGCATT+TGG - chr2.4:3381631-3381650 MS.gene85141:CDS 45.0%
CTCTGGTGGTTCAATGAATC+CGG - chr2.4:3382519-3382538 MS.gene85141:intron 45.0%
CTTTCAGTGTGAGCTCCATT+AGG - chr2.4:3381668-3381687 MS.gene85141:CDS 45.0%
GACATGTCCACCAGATATGT+TGG + chr2.4:3382075-3382094 None:intergenic 45.0%
GTGGTGACTACCATAACAAC+TGG - chr2.4:3382569-3382588 MS.gene85141:CDS 45.0%
TAAACATACCAAGCCTCCTG+TGG + chr2.4:3382216-3382235 None:intergenic 45.0%
TAGTCACCACTTACAACTGC+AGG + chr2.4:3382559-3382578 None:intergenic 45.0%
TCTGTTGGAGCCAACATATC+TGG - chr2.4:3382062-3382081 MS.gene85141:intron 45.0%
TGAATCCGGCACGTTCATTT+GGG - chr2.4:3382533-3382552 MS.gene85141:intron 45.0%
TTTCTCCTCAAGTTTGCCAC+AGG - chr2.4:3382197-3382216 MS.gene85141:CDS 45.0%
TTTCTTAGGATCAGCTGCTG+TGG + chr2.4:3382425-3382444 None:intergenic 45.0%
!! ACCTCTTATTGGTGGTGGTT+TGG - chr2.4:3382606-3382625 MS.gene85141:CDS 45.0%
!!! ATTTTTTTATTATACTATTT+AGG + chr2.4:3381883-3381902 None:intergenic 5.0%
AATAGCCAACCCGAAGGTCA+CGG + chr2.4:3382105-3382124 None:intergenic 50.0%
CAACACCAGAAGCAACACTG+TGG + chr2.4:3382339-3382358 None:intergenic 50.0%
CCAGAAGCAACACTGTGGAT+AGG + chr2.4:3382334-3382353 None:intergenic 50.0%
CTCAGCAGCATAAGCCCTAA+TGG + chr2.4:3381686-3381705 None:intergenic 50.0%
GCAACTGCTACTAATCCAGC+AGG + chr2.4:3382008-3382027 None:intergenic 50.0%
GCCAAACCACCACCAATAAG+AGG + chr2.4:3382610-3382629 None:intergenic 50.0%
GGACAGATCACTATCCTCAC+CGG - chr2.4:3382128-3382147 MS.gene85141:CDS 50.0%
GTTGGACCTCTTATTGGTGG+TGG - chr2.4:3382601-3382620 MS.gene85141:CDS 50.0%
GTTGGAGCCAACATATCTGG+TGG - chr2.4:3382065-3382084 MS.gene85141:intron 50.0%
TCCAGTAGAAGATACCGGTG+AGG + chr2.4:3382145-3382164 None:intergenic 50.0%
TCCTCACCGGTATCTTCTAC+TGG - chr2.4:3382141-3382160 MS.gene85141:CDS 50.0%
TGGTCCTGCAGCTAAGATGT+TGG + chr2.4:3382497-3382516 None:intergenic 50.0%
TTAGCTGCAGGACCATTCTC+TGG - chr2.4:3382502-3382521 MS.gene85141:intron 50.0%
TTTGGGCCTGCAGTTGTAAG+TGG - chr2.4:3382550-3382569 MS.gene85141:intron 50.0%
! AGCCAAAGGTACATGGTCTG+AGG + chr2.4:3382662-3382681 None:intergenic 50.0%
! GAGGAGAAAGCATGCCACTA+TGG + chr2.4:3382186-3382205 None:intergenic 50.0%
! GCCAAAGGTACATGGTCTGA+GGG + chr2.4:3382661-3382680 None:intergenic 50.0%
! GTAGCAGTTGCCGTTTGTCA+TGG - chr2.4:3382017-3382036 MS.gene85141:intron 50.0%
! GTTGGTTCAGCCATAGCCTA+TGG - chr2.4:3381739-3381758 MS.gene85141:CDS 50.0%
! TGGGTTGGACCTCTTATTGG+TGG - chr2.4:3382598-3382617 MS.gene85141:CDS 50.0%
!! CACAGTGTTGCTTCTGGTGT+TGG - chr2.4:3382337-3382356 MS.gene85141:intron 50.0%
!! CCTATCCACAGTGTTGCTTC+TGG - chr2.4:3382331-3382350 MS.gene85141:intron 50.0%
!! CTTATTGGTGGTGGTTTGGC+TGG - chr2.4:3382610-3382629 MS.gene85141:CDS 50.0%
!! GGTTTGGCTGGTGTCATCTA+TGG - chr2.4:3382622-3382641 MS.gene85141:CDS 50.0%
!! TGACCTTCGGGTTGGCTATT+GGG - chr2.4:3382105-3382124 MS.gene85141:CDS 50.0%
AGCCTCCTGTGGCAAACTTG+AGG + chr2.4:3382205-3382224 None:intergenic 55.0%
CAAGTTTGCCACAGGAGGCT+TGG - chr2.4:3382205-3382224 MS.gene85141:CDS 55.0%
CTCCTCAAGTTTGCCACAGG+AGG - chr2.4:3382200-3382219 MS.gene85141:CDS 55.0%
GCCCTCAGACCATGTACCTT+TGG - chr2.4:3382657-3382676 MS.gene85141:CDS 55.0%
GGTGCCAACATCTTAGCTGC+AGG - chr2.4:3382490-3382509 MS.gene85141:intron 55.0%
! GTTGCTTCTGGTGTTGGAGC+TGG - chr2.4:3382343-3382362 MS.gene85141:intron 55.0%
! TCTGGTGTTGGAGCTGGTGA+AGG - chr2.4:3382349-3382368 MS.gene85141:intron 55.0%
!! ACCTTCGGGTTGGCTATTGG+GGG - chr2.4:3382107-3382126 MS.gene85141:CDS 55.0%
!! GACCTTCGGGTTGGCTATTG+GGG - chr2.4:3382106-3382125 MS.gene85141:CDS 55.0%
!! GTGACCTTCGGGTTGGCTAT+TGG - chr2.4:3382104-3382123 MS.gene85141:CDS 55.0%
GCAGGCCCAAATGAACGTGC+CGG + chr2.4:3382541-3382560 None:intergenic 60.0%
GCTGCAGGACCATTCTCTGG+TGG - chr2.4:3382505-3382524 MS.gene85141:intron 60.0%
GGTGCAGCTCTTGATCCTGC+TGG - chr2.4:3381990-3382009 MS.gene85141:intron 60.0%
GTCAACCCAGCCGTGACCTT+CGG - chr2.4:3382092-3382111 MS.gene85141:intron 60.0%
TCAACCCAGCCGTGACCTTC+GGG - chr2.4:3382093-3382112 MS.gene85141:intron 60.0%
TCCCCCAATAGCCAACCCGA+AGG + chr2.4:3382111-3382130 None:intergenic 60.0%
CCAACCCGAAGGTCACGGCT+GGG + chr2.4:3382100-3382119 None:intergenic 65.0%
CCCAGCCGTGACCTTCGGGT+TGG - chr2.4:3382097-3382116 MS.gene85141:intron 70.0%
GCCAACCCGAAGGTCACGGC+TGG + chr2.4:3382101-3382120 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 3381631 3382693 3381631 ID=MS.gene85141
chr2.4 mRNA 3381631 3382693 3381631 ID=MS.gene85141.t1;Parent=MS.gene85141
chr2.4 exon 3382564 3382693 3382564 ID=MS.gene85141.t1.exon1;Parent=MS.gene85141.t1
chr2.4 CDS 3382564 3382693 3382564 ID=cds.MS.gene85141.t1;Parent=MS.gene85141.t1
chr2.4 exon 3382098 3382348 3382098 ID=MS.gene85141.t1.exon2;Parent=MS.gene85141.t1
chr2.4 CDS 3382098 3382348 3382098 ID=cds.MS.gene85141.t1;Parent=MS.gene85141.t1
chr2.4 exon 3381631 3381999 3381631 ID=MS.gene85141.t1.exon3;Parent=MS.gene85141.t1
chr2.4 CDS 3381631 3381999 3381631 ID=cds.MS.gene85141.t1;Parent=MS.gene85141.t1
Gene Sequence

>MS.gene85141

ATGGGTCATGGCATAGCATTTGGACGCTTTGATGATTCTTTCAGTGTGAGCTCCATTAGGGCTTATGCTGCTGAGTTCATCTCAACCTTGATCTTTGTTTTTGCTGGTGTTGGTTCAGCCATAGCCTATGGTTAGTTTTTTCTTCTTTACAAATGAATTAAACCAAATCAAGATTATACATGACCATGAAACTTCTAAAGTAATACATTGTTTTTACCTTGATAAAATATTTCATTGAAGAAAATGACACCTAAATAGTATAATAAAAAAATGTAGACTATATGTTGCAAATAACAAAGTTGGAGTTATGGAAACCAAACACTTAAAAACTTTGTACTTTTGCAGCTAAGTTGACATCAGGTGCAGCTCTTGATCCTGCTGGATTAGTAGCAGTTGCCGTTTGTCATGGTTTTGCTCTTTTTGTTGCTGTTTCTGTTGGAGCCAACATATCTGGTGGACATGTCAACCCAGCCGTGACCTTCGGGTTGGCTATTGGGGGACAGATCACTATCCTCACCGGTATCTTCTACTGGATTGCACAACTTCTTGGTTCCATAGTGGCATGCTTTCTCCTCAAGTTTGCCACAGGAGGCTTGGTATGTTTATCATGCTTGCATCACAAGTTTCATACCTTTTCGAATTTTAAAACATCAATAGTTCCATAACTGACTGTATCTTATGTATTTGTTTTCAGACAATTCCTATCCACAGTGTTGCTTCTGGTGTTGGAGCTGGTGAAGGAGTTGTGACAGAAATTATCATAACATTTGGATTGGTGTACACAGTATATGCCACAGCAGCTGATCCTAAGAAAGGATCATTAGGGACAATTGCACCAATTGCTATTGGTTTGATTGTTGGTGCCAACATCTTAGCTGCAGGACCATTCTCTGGTGGTTCAATGAATCCGGCACGTTCATTTGGGCCTGCAGTTGTAAGTGGTGACTACCATAACAACTGGATCTATTGGGTTGGACCTCTTATTGGTGGTGGTTTGGCTGGTGTCATCTATGGCTATGTCTTCATGCCCTCAGACCATGTACCTTTGGCTAGTGATTTTTGA
Protein sequence

>MS.gene85141.t1

MGHGIAFGRFDDSFSVSSIRAYAAEFISTLIFVFAGVGSAIAYAKLTSGAALDPAGLVAVAVCHGFALFVAVSVGANISGGHVNPAVTFGLAIGGQITILTGIFYWIAQLLGSIVACFLLKFATGGLTIPIHSVASGVGAGEGVVTEIIITFGLVYTVYATAADPKKGSLGTIAPIAIGLIVGANILAAGPFSGGSMNPARSFGPAVVSGDYHNNWIYWVGPLIGGGLAGVIYGYVFMPSDHVPLASDF