AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025449

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025449.t1 MTR_2g060360 98.104 211 4 0 4 214 44 254 1.08e-147 411
MS.gene025449.t1 MTR_8g013680 47.000 200 105 1 3 201 43 242 3.31e-62 194
MS.gene025449.t1 MTR_2g101370 46.970 198 104 1 4 200 45 242 1.91e-58 184
MS.gene025449.t1 MTR_8g468730 76.271 118 10 1 97 214 1 100 1.11e-51 162
MS.gene025449.t1 MTR_5g012810 48.705 193 97 2 4 195 44 235 2.74e-51 166
MS.gene025449.t1 MTR_4g063090 41.089 202 118 1 4 204 46 247 1.91e-49 162
MS.gene025449.t1 MTR_1g115405 40.777 206 115 2 4 202 46 251 4.34e-49 160
MS.gene025449.t1 MTR_7g023350 40.291 206 116 2 4 202 46 251 2.97e-47 156
MS.gene025449.t1 MTR_1g077540 42.132 197 113 1 4 199 46 242 4.28e-46 153
MS.gene025449.t1 MTR_1g006590 41.667 168 97 1 27 193 63 230 9.71e-42 142
MS.gene025449.t1 MTR_6g069040 43.030 165 93 1 36 199 78 242 2.10e-41 141
MS.gene025449.t1 MTR_6g044390 67.521 117 5 1 97 213 1 84 3.94e-41 135
MS.gene025449.t1 MTR_7g103030 41.584 202 115 3 4 204 46 245 9.44e-41 139
MS.gene025449.t1 MTR_1g006490 40.323 186 106 3 20 204 57 238 1.13e-40 139
MS.gene025449.t1 MTR_5g063930 34.896 192 119 3 13 199 64 254 3.07e-23 94.4
MS.gene025449.t1 MTR_8g006915 60.227 88 2 1 127 214 18 72 2.28e-22 86.7
MS.gene025449.t1 MTR_7g101190 35.912 181 97 5 30 195 94 270 7.87e-21 88.2
MS.gene025449.t1 MTR_2g094270 35.870 184 93 5 30 195 96 272 2.72e-20 86.7
MS.gene025449.t1 MTR_4g059390 37.222 180 96 5 30 195 94 270 3.28e-19 84.0
MS.gene025449.t1 MTR_4g415300 32.536 209 112 6 8 195 81 281 4.18e-18 80.9
MS.gene025449.t1 MTR_5g010150 32.836 201 109 7 15 195 84 278 2.88e-17 78.6
MS.gene025449.t1 MTR_3g070210 33.505 194 101 7 29 200 103 290 3.62e-17 78.2
MS.gene025449.t1 MTR_5g082070 32.474 194 105 6 18 191 90 277 3.68e-17 78.2
MS.gene025449.t1 MTR_1g095070 32.973 185 97 4 30 195 28 204 4.19e-17 77.0
MS.gene025449.t1 MTR_3g118010 33.889 180 102 4 30 195 86 262 9.56e-17 77.0
MS.gene025449.t1 MTR_8g098375 32.432 185 101 6 29 195 100 278 2.47e-16 75.9
MS.gene025449.t1 MTR_5g079570 33.523 176 111 4 32 203 87 260 1.78e-15 73.6
MS.gene025449.t1 MTR_1g097840 33.516 182 101 5 36 205 126 299 3.10e-15 73.2
MS.gene025449.t1 MTR_8g087710 31.868 182 119 3 20 199 75 253 3.33e-15 72.8
MS.gene025449.t1 MTR_7g010650 33.333 183 100 6 12 184 65 235 6.18e-15 72.0
MS.gene025449.t1 MTR_3g008920 30.769 156 107 1 37 192 78 232 1.32e-14 70.9
MS.gene025449.t1 MTR_4g084880 30.000 170 118 1 30 199 83 251 2.88e-14 70.1
MS.gene025449.t1 MTR_8g087720 30.636 173 113 3 30 199 84 252 6.45e-14 68.9
MS.gene025449.t1 MTR_2g017590 32.335 167 108 3 20 184 72 235 1.00e-13 68.6
MS.gene025449.t1 MTR_2g017620 30.055 183 122 3 30 211 3 180 1.53e-13 66.6
MS.gene025449.t1 MTR_4g006730 29.524 210 132 5 12 213 16 217 1.57e-13 67.4
MS.gene025449.t1 MTR_4g006630 29.524 210 132 5 12 213 16 217 2.40e-13 66.6
MS.gene025449.t1 MTR_4g006730 29.524 210 132 5 12 213 103 304 2.50e-13 67.8
MS.gene025449.t1 MTR_2g104940 29.208 202 127 5 15 209 111 303 2.51e-13 67.8
MS.gene025449.t1 MTR_2g017660 30.636 173 119 1 20 192 75 246 2.89e-13 67.4
MS.gene025449.t1 MTR_1g070385 30.952 168 112 4 37 203 115 279 3.63e-13 68.2
MS.gene025449.t1 MTR_2g094270 34.568 162 81 5 30 176 96 247 4.49e-13 66.6
MS.gene025449.t1 MTR_2g017620 30.435 184 120 4 30 211 70 247 5.41e-13 66.2
MS.gene025449.t1 MTR_2g017620 30.435 184 120 4 30 211 84 261 6.22e-13 66.2
MS.gene025449.t1 MTR_3g070210 33.987 153 80 5 29 166 103 249 2.36e-11 62.0
MS.gene025449.t1 MTR_1g095070 33.987 153 77 3 30 166 28 172 3.00e-11 60.5
MS.gene025449.t1 MTR_2g017610 29.474 190 122 4 30 210 84 270 3.16e-11 61.6
MS.gene025449.t1 MTR_2g017570 29.534 193 124 5 30 213 84 273 6.30e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025449.t1 AT3G47440 63.768 207 72 1 4 210 48 251 7.46e-90 265
MS.gene025449.t1 AT3G16240 51.515 198 95 1 4 200 44 241 1.27e-60 190
MS.gene025449.t1 AT5G47450 46.798 203 107 1 4 205 44 246 2.61e-57 182
MS.gene025449.t1 AT4G17340 48.293 205 101 2 4 203 44 248 1.11e-53 172
MS.gene025449.t1 AT4G01470 42.857 203 115 1 3 204 45 247 8.46e-52 167
MS.gene025449.t1 AT2G36830 43.564 202 112 2 4 204 46 246 2.86e-50 164
MS.gene025449.t1 AT3G26520 41.063 207 120 2 4 209 47 252 4.62e-48 158
MS.gene025449.t1 AT1G17810 46.707 167 88 1 36 201 86 252 1.16e-46 155
MS.gene025449.t1 AT2G25810 46.875 160 84 1 37 195 73 232 4.27e-44 148
MS.gene025449.t1 AT1G73190 43.636 165 92 1 36 199 86 250 1.84e-43 147
MS.gene025449.t1 AT5G60660 35.000 180 100 4 30 195 94 270 6.09e-21 88.6
MS.gene025449.t1 AT3G53420 35.000 180 100 4 30 195 94 270 6.57e-21 88.6
MS.gene025449.t1 AT3G53420 35.000 180 100 4 30 195 94 270 6.57e-21 88.6
MS.gene025449.t1 AT2G39010 35.955 178 101 4 30 195 93 269 2.13e-19 84.3
MS.gene025449.t1 AT2G37170 34.444 180 101 4 30 195 92 268 6.41e-19 83.2
MS.gene025449.t1 AT2G37180 34.444 180 101 4 30 195 92 268 9.06e-19 82.8
MS.gene025449.t1 AT2G37170 34.444 180 101 4 30 195 135 311 9.72e-19 83.2
MS.gene025449.t1 AT4G35100 33.880 183 98 5 30 195 87 263 1.47e-18 82.0
MS.gene025449.t1 AT4G35100 33.880 183 98 5 30 195 87 263 1.47e-18 82.0
MS.gene025449.t1 AT4G23400 33.503 197 112 5 15 195 85 278 2.06e-18 81.6
MS.gene025449.t1 AT3G54820 32.057 209 124 6 4 195 62 269 4.35e-18 80.9
MS.gene025449.t1 AT3G61430 34.518 197 102 7 15 191 84 273 7.01e-18 80.5
MS.gene025449.t1 AT3G61430 34.518 197 102 7 15 191 84 273 7.01e-18 80.5
MS.gene025449.t1 AT2G16850 33.880 183 98 5 30 195 85 261 7.08e-18 80.1
MS.gene025449.t1 AT4G00430 33.333 201 107 7 15 195 85 278 9.43e-18 80.1
MS.gene025449.t1 AT1G01620 33.831 201 106 7 15 195 12 205 1.42e-17 78.2
MS.gene025449.t1 AT2G45960 34.518 197 102 7 15 191 84 273 1.80e-17 79.3
MS.gene025449.t1 AT1G01620 33.831 201 106 7 15 195 84 277 2.09e-17 79.0
MS.gene025449.t1 AT1G52180 41.489 94 54 1 78 170 28 121 2.19e-15 70.1
MS.gene025449.t1 AT4G10380 31.156 199 123 4 15 207 109 299 3.39e-15 73.2
MS.gene025449.t1 AT5G37820 31.765 170 115 1 30 199 89 257 4.67e-14 69.7
MS.gene025449.t1 AT1G31885 29.091 220 132 5 12 211 17 232 5.94e-14 69.3
MS.gene025449.t1 AT1G80760 32.812 192 114 5 17 201 112 295 6.13e-14 69.7
MS.gene025449.t1 AT5G37820 31.765 170 115 1 30 199 125 293 6.89e-14 69.7
MS.gene025449.t1 AT1G31885 30.198 202 117 5 30 211 89 286 1.05e-13 68.9
MS.gene025449.t1 AT5G37810 30.000 170 118 1 30 199 89 257 1.27e-13 68.6
MS.gene025449.t1 AT3G06100 28.889 180 127 1 20 199 82 260 1.69e-13 68.2
MS.gene025449.t1 AT3G06100 28.889 180 127 1 20 199 34 212 2.71e-13 67.0
MS.gene025449.t1 AT4G18910 30.387 181 110 5 30 199 98 273 7.12e-13 66.6
MS.gene025449.t1 AT4G19030 27.368 190 124 4 20 199 91 276 5.57e-12 63.9
MS.gene025449.t1 AT2G45960 34.320 169 87 6 15 166 84 245 9.71e-12 63.2
MS.gene025449.t1 AT2G45960 34.320 169 87 6 15 166 84 245 1.40e-11 62.8

Find 63 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAATTCTGATTCTATTAG+AGG 0.193327 4.2:+61562783 MS.gene025449:CDS
TTCGGATCTGCTTCCATTGC+CGG 0.254900 4.2:+61562669 MS.gene025449:CDS
TCAACCGGAATACTTGTAAT+TGG 0.260234 4.2:+61562576 MS.gene025449:CDS
TTGGTTGGTGCTGCTGTTGC+TGG 0.268882 4.2:+61562729 MS.gene025449:CDS
TCAGGAATCAAGCTGTCTAT+TGG 0.284879 4.2:+61562697 MS.gene025449:CDS
GCGGAAGAGATGACAGGATT+TGG 0.318727 4.2:+61562468 MS.gene025449:CDS
CTTCCATTGCCGGAACATTC+AGG 0.324826 4.2:+61562679 MS.gene025449:CDS
TGTCTATTGGGTTGGTCCTT+TGG 0.325188 4.2:+61562710 MS.gene025449:CDS
TTCATTGAACCCAACAAGTT+TGG 0.331866 4.2:+61562086 MS.gene025449:CDS
TAAACCAATTACAAGTATTC+CGG 0.338819 4.2:-61562580 None:intergenic
ATCAACCCTGCATGTGCTTT+CGG 0.346124 4.2:+61562651 MS.gene025449:CDS
GGGTTTCAGATGGAACTGTT+AGG 0.373541 4.2:+61562805 MS.gene025449:CDS
TACATTGCATGGGACATATC+TGG 0.375387 4.2:+61562153 MS.gene025449:CDS
CAGGAATCAAGCTGTCTATT+GGG 0.383404 4.2:+61562698 MS.gene025449:CDS
CTTGTAATTGGTTTAATAGC+AGG 0.406060 4.2:+61562588 MS.gene025449:CDS
TTCCTCAAAGTCATCGTTGT+CGG 0.414197 4.2:+61562291 MS.gene025449:CDS
ATTTGGAGCATCAATATTAG+AGG 0.429331 4.2:+61562485 MS.gene025449:CDS
GCACCCACAACCAAACTTGT+TGG 0.436780 4.2:-61562096 None:intergenic
TGCCGACAACGATGACTTTG+AGG 0.450861 4.2:-61562293 None:intergenic
CACCCACAACCAAACTTGTT+GGG 0.455571 4.2:-61562095 None:intergenic
CAACGATGACTTTGAGGAAA+AGG 0.457590 4.2:-61562287 None:intergenic
CGTGGTCAAGTAAGTTCAAC+CGG 0.461078 4.2:+61562561 MS.gene025449:CDS
GGTTTCAGATGGAACTGTTA+GGG 0.464680 4.2:+61562806 MS.gene025449:CDS
TTAGCAGCAGGTCCATTCTC+TGG 0.467463 4.2:+61562621 MS.gene025449:CDS
TGTTACCTTTGCAATGGCTG+TGG 0.474139 4.2:+61562194 MS.gene025449:CDS
ATTCCTGAATGTTCCGGCAA+TGG 0.481196 4.2:-61562682 None:intergenic
TATTGGGTTGGTCCTTTGGT+TGG 0.482215 4.2:+61562714 MS.gene025449:CDS
ACCCAATAAAAGAGAGCAGT+TGG 0.491942 4.2:-61562234 None:intergenic
GTTGGGTTCAATGAAGCGTC+CGG 0.494246 4.2:-61562078 None:intergenic
TTGATTGACCCACCAGAGAA+TGG 0.496422 4.2:-61562633 None:intergenic
AGAATTCTGATTCTATTAGA+GGG 0.496583 4.2:+61562784 MS.gene025449:CDS
GAACCCAACAAGTTTGGTTG+TGG 0.516159 4.2:+61562092 MS.gene025449:CDS
GGAGCAAGTGTGTTAGCAGC+AGG 0.517606 4.2:+61562609 MS.gene025449:CDS
TCCTGCTGTTACCTTTGCAA+TGG 0.517646 4.2:+61562188 MS.gene025449:CDS
CCCAATAAAAGAGAGCAGTT+GGG 0.517838 4.2:-61562233 None:intergenic
TCTATTAGAGGGGTTTCAGA+TGG 0.522207 4.2:+61562795 MS.gene025449:CDS
GCAGATCCGAAAGCACATGC+AGG 0.523257 4.2:-61562657 None:intergenic
GTTACCTTTGCAATGGCTGT+GGG 0.523279 4.2:+61562195 MS.gene025449:CDS
AGCTTGATTCCTGAATGTTC+CGG 0.523609 4.2:-61562688 None:intergenic
TGTACACAATTTACGCTGCA+AGG 0.530874 4.2:+61562529 MS.gene025449:CDS
GCAACAGCAGCACCAACCAA+AGG 0.531682 4.2:-61562726 None:intergenic
CAAAGTCATCGTTGTCGGCA+TGG 0.534604 4.2:+61562296 MS.gene025449:CDS
GCCATTGCAAAGGTAACAGC+AGG 0.535121 4.2:-61562189 None:intergenic
GCAGCAGGTCCATTCTCTGG+TGG 0.535592 4.2:+61562624 MS.gene025449:CDS
AACCCAACAAGTTTGGTTGT+GGG 0.547817 4.2:+61562093 MS.gene025449:CDS
TCTTCCGCAATTGAATATGT+TGG 0.549444 4.2:-61562453 None:intergenic
TTTACGCTGCAAGGGACACT+AGG 0.555338 4.2:+61562538 MS.gene025449:CDS
CAGCAGGTCCATTCTCTGGT+GGG 0.565386 4.2:+61562625 MS.gene025449:CDS
TCAATTGCGGAAGAGATGAC+AGG 0.567654 4.2:+61562462 MS.gene025449:CDS
ATAGAATCAGAATTCTGAGA+AGG 0.587904 4.2:-61562777 None:intergenic
AATCAAGCTGTCTATTGGGT+TGG 0.591734 4.2:+61562702 MS.gene025449:CDS
AATGACAGGGAAATTGATGC+CGG 0.594954 4.2:+61562059 None:intergenic
CAGAGCAAAAGCACTTGCAA+TGG 0.601303 4.2:-61562118 None:intergenic
GTACACAATTTACGCTGCAA+GGG 0.602826 4.2:+61562530 MS.gene025449:CDS
TTTGGAGCATCAATATTAGA+GGG 0.603307 4.2:+61562486 MS.gene025449:CDS
GAATTCTGATTCTATTAGAG+GGG 0.605960 4.2:+61562785 MS.gene025449:CDS
TCCTCCCACAGCCATTGCAA+AGG 0.611836 4.2:-61562199 None:intergenic
ACCTTTGCAATGGCTGTGGG+AGG 0.626324 4.2:+61562198 MS.gene025449:CDS
CAGATCCGAAAGCACATGCA+GGG 0.630066 4.2:-61562656 None:intergenic
ATTGCATGGGACATATCTGG+TGG 0.647153 4.2:+61562156 MS.gene025449:CDS
TGTGCCAACATATTCAATTG+CGG 0.676658 4.2:+61562449 MS.gene025449:CDS
GCTGCAAGGGACACTAGGCG+TGG 0.685457 4.2:+61562543 MS.gene025449:CDS
GAAAAGGCAAGCAATAACAG+AGG 0.705928 4.2:-61562271 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAATTCTGATTCTATTAGA+GGG + chr4.2:61562784-61562803 MS.gene025449:CDS 25.0%
! CTATAGTACTTATGCAATAA+TGG + chr4.2:61562396-61562415 MS.gene025449:intron 25.0%
! TAAACCAATTACAAGTATTC+CGG - chr4.2:61562583-61562602 None:intergenic 25.0%
! TTGATGTTGAGCATATAAAA+GGG - chr4.2:61562339-61562358 None:intergenic 25.0%
! TTTGATGTTGAGCATATAAA+AGG - chr4.2:61562340-61562359 None:intergenic 25.0%
CAGAATTCTGATTCTATTAG+AGG + chr4.2:61562783-61562802 MS.gene025449:CDS 30.0%
GAATTCTGATTCTATTAGAG+GGG + chr4.2:61562785-61562804 MS.gene025449:CDS 30.0%
TGATGTTGAGCATATAAAAG+GGG - chr4.2:61562338-61562357 None:intergenic 30.0%
! ATAGAATCAGAATTCTGAGA+AGG - chr4.2:61562780-61562799 None:intergenic 30.0%
! ATTTGGAGCATCAATATTAG+AGG + chr4.2:61562485-61562504 MS.gene025449:CDS 30.0%
! CTTGTAATTGGTTTAATAGC+AGG + chr4.2:61562588-61562607 MS.gene025449:CDS 30.0%
! TTCTGTTTTGTACATTGCAT+GGG + chr4.2:61562143-61562162 MS.gene025449:CDS 30.0%
! TTTGGAGCATCAATATTAGA+GGG + chr4.2:61562486-61562505 MS.gene025449:CDS 30.0%
CATATAAAAGGGGTAAGGTA+AGG - chr4.2:61562328-61562347 None:intergenic 35.0%
TCAACCGGAATACTTGTAAT+TGG + chr4.2:61562576-61562595 MS.gene025449:CDS 35.0%
TCTTCCGCAATTGAATATGT+TGG - chr4.2:61562456-61562475 None:intergenic 35.0%
TGTGCCAACATATTCAATTG+CGG + chr4.2:61562449-61562468 MS.gene025449:CDS 35.0%
TTCATTGAACCCAACAAGTT+TGG + chr4.2:61562086-61562105 MS.gene025449:CDS 35.0%
TTGAGCATATAAAAGGGGTA+AGG - chr4.2:61562333-61562352 None:intergenic 35.0%
! CTTCTGTTTTGTACATTGCA+TGG + chr4.2:61562142-61562161 MS.gene025449:CDS 35.0%
AACCCAACAAGTTTGGTTGT+GGG + chr4.2:61562093-61562112 MS.gene025449:CDS 40.0%
AATCAAGCTGTCTATTGGGT+TGG + chr4.2:61562702-61562721 MS.gene025449:CDS 40.0%
ACCCAATAAAAGAGAGCAGT+TGG - chr4.2:61562237-61562256 None:intergenic 40.0%
AGCTTGATTCCTGAATGTTC+CGG - chr4.2:61562691-61562710 None:intergenic 40.0%
CAGGAATCAAGCTGTCTATT+GGG + chr4.2:61562698-61562717 MS.gene025449:CDS 40.0%
CCCAATAAAAGAGAGCAGTT+GGG - chr4.2:61562236-61562255 None:intergenic 40.0%
GAAAAGGCAAGCAATAACAG+AGG - chr4.2:61562274-61562293 None:intergenic 40.0%
GGTTTCAGATGGAACTGTTA+GGG + chr4.2:61562806-61562825 MS.gene025449:CDS 40.0%
GTACACAATTTACGCTGCAA+GGG + chr4.2:61562530-61562549 MS.gene025449:CDS 40.0%
TACATTGCATGGGACATATC+TGG + chr4.2:61562153-61562172 MS.gene025449:CDS 40.0%
TCAGGAATCAAGCTGTCTAT+TGG + chr4.2:61562697-61562716 MS.gene025449:CDS 40.0%
TCTATTAGAGGGGTTTCAGA+TGG + chr4.2:61562795-61562814 MS.gene025449:CDS 40.0%
TGTACACAATTTACGCTGCA+AGG + chr4.2:61562529-61562548 MS.gene025449:CDS 40.0%
TTCCTCAAAGTCATCGTTGT+CGG + chr4.2:61562291-61562310 MS.gene025449:CDS 40.0%
! CAACGATGACTTTGAGGAAA+AGG - chr4.2:61562290-61562309 None:intergenic 40.0%
! CCCAACTGCTCTCTTTTATT+GGG + chr4.2:61562233-61562252 MS.gene025449:CDS 40.0%
! TCCCAACTGCTCTCTTTTAT+TGG + chr4.2:61562232-61562251 MS.gene025449:CDS 40.0%
ATCAACCCTGCATGTGCTTT+CGG + chr4.2:61562651-61562670 MS.gene025449:CDS 45.0%
ATTCCTGAATGTTCCGGCAA+TGG - chr4.2:61562685-61562704 None:intergenic 45.0%
ATTGCATGGGACATATCTGG+TGG + chr4.2:61562156-61562175 MS.gene025449:CDS 45.0%
CGTGGTCAAGTAAGTTCAAC+CGG + chr4.2:61562561-61562580 MS.gene025449:CDS 45.0%
GAACCCAACAAGTTTGGTTG+TGG + chr4.2:61562092-61562111 MS.gene025449:CDS 45.0%
GGGTTTCAGATGGAACTGTT+AGG + chr4.2:61562805-61562824 MS.gene025449:CDS 45.0%
GTTACCTTTGCAATGGCTGT+GGG + chr4.2:61562195-61562214 MS.gene025449:CDS 45.0%
TCAATTGCGGAAGAGATGAC+AGG + chr4.2:61562462-61562481 MS.gene025449:CDS 45.0%
TCCTGCTGTTACCTTTGCAA+TGG + chr4.2:61562188-61562207 MS.gene025449:CDS 45.0%
TGTTACCTTTGCAATGGCTG+TGG + chr4.2:61562194-61562213 MS.gene025449:CDS 45.0%
TTGATTGACCCACCAGAGAA+TGG - chr4.2:61562636-61562655 None:intergenic 45.0%
! CACCCACAACCAAACTTGTT+GGG - chr4.2:61562098-61562117 None:intergenic 45.0%
!! CAGAGCAAAAGCACTTGCAA+TGG - chr4.2:61562121-61562140 None:intergenic 45.0%
!! TATTGGGTTGGTCCTTTGGT+TGG + chr4.2:61562714-61562733 MS.gene025449:CDS 45.0%
!! TGTCTATTGGGTTGGTCCTT+TGG + chr4.2:61562710-61562729 MS.gene025449:CDS 45.0%
CAAAGTCATCGTTGTCGGCA+TGG + chr4.2:61562296-61562315 MS.gene025449:CDS 50.0%
CTTCCATTGCCGGAACATTC+AGG + chr4.2:61562679-61562698 MS.gene025449:CDS 50.0%
GCACCCACAACCAAACTTGT+TGG - chr4.2:61562099-61562118 None:intergenic 50.0%
GCCATTGCAAAGGTAACAGC+AGG - chr4.2:61562192-61562211 None:intergenic 50.0%
GCGGAAGAGATGACAGGATT+TGG + chr4.2:61562468-61562487 MS.gene025449:CDS 50.0%
TGCCGACAACGATGACTTTG+AGG - chr4.2:61562296-61562315 None:intergenic 50.0%
TTAGCAGCAGGTCCATTCTC+TGG + chr4.2:61562621-61562640 MS.gene025449:CDS 50.0%
TTCGGATCTGCTTCCATTGC+CGG + chr4.2:61562669-61562688 MS.gene025449:CDS 50.0%
TTTACGCTGCAAGGGACACT+AGG + chr4.2:61562538-61562557 MS.gene025449:CDS 50.0%
! CAGATCCGAAAGCACATGCA+GGG - chr4.2:61562659-61562678 None:intergenic 50.0%
! GTTGGGTTCAATGAAGCGTC+CGG - chr4.2:61562081-61562100 None:intergenic 50.0%
ACCTTTGCAATGGCTGTGGG+AGG + chr4.2:61562198-61562217 MS.gene025449:CDS 55.0%
CAGCAGGTCCATTCTCTGGT+GGG + chr4.2:61562625-61562644 MS.gene025449:CDS 55.0%
GCAACAGCAGCACCAACCAA+AGG - chr4.2:61562729-61562748 None:intergenic 55.0%
GGAGCAAGTGTGTTAGCAGC+AGG + chr4.2:61562609-61562628 MS.gene025449:CDS 55.0%
TCCTCCCACAGCCATTGCAA+AGG - chr4.2:61562202-61562221 None:intergenic 55.0%
! GCAGATCCGAAAGCACATGC+AGG - chr4.2:61562660-61562679 None:intergenic 55.0%
!! TTGGTTGGTGCTGCTGTTGC+TGG + chr4.2:61562729-61562748 MS.gene025449:CDS 55.0%
GCAGCAGGTCCATTCTCTGG+TGG + chr4.2:61562624-61562643 MS.gene025449:CDS 60.0%
GCTGCAAGGGACACTAGGCG+TGG + chr4.2:61562543-61562562 MS.gene025449:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 61562060 61562833 61562060 ID=MS.gene025449
chr4.2 mRNA 61562060 61562833 61562060 ID=MS.gene025449.t1;Parent=MS.gene025449
chr4.2 exon 61562060 61562317 61562060 ID=MS.gene025449.t1.exon1;Parent=MS.gene025449.t1
chr4.2 CDS 61562060 61562317 61562060 ID=cds.MS.gene025449.t1;Parent=MS.gene025449.t1
chr4.2 exon 61562447 61562833 61562447 ID=MS.gene025449.t1.exon2;Parent=MS.gene025449.t1
chr4.2 CDS 61562447 61562833 61562447 ID=cds.MS.gene025449.t1;Parent=MS.gene025449.t1
Gene Sequence

>MS.gene025449

ATGACAGGGAAATTGATGCCGGACGCTTCATTGAACCCAACAAGTTTGGTTGTGGGTGCCATTGCAAGTGCTTTTGCTCTGTCTTCTGTTTTGTACATTGCATGGGACATATCTGGTGGACATGTCAATCCTGCTGTTACCTTTGCAATGGCTGTGGGAGGACATATTAGTGTCCCAACTGCTCTCTTTTATTGGGTTGCTCAACTTATTGCCTCTGTTATTGCTTGCCTTTTCCTCAAAGTCATCGTTGTCGGCATGGTATTTTCCTTACCTTACCCCTTTTATATGCTCAACATCAAAATTTCAATTTTATCGAAATTTTGTAGTTGATTGTATCTATAGTACTTATGCAATAATGGTTCATACACTCGTGTATATTTTTTGCAGCATGTGCCAACATATTCAATTGCGGAAGAGATGACAGGATTTGGAGCATCAATATTAGAGGGTATACTAACATTTGTGTTAGTGTACACAATTTACGCTGCAAGGGACACTAGGCGTGGTCAAGTAAGTTCAACCGGAATACTTGTAATTGGTTTAATAGCAGGAGCAAGTGTGTTAGCAGCAGGTCCATTCTCTGGTGGGTCAATCAACCCTGCATGTGCTTTCGGATCTGCTTCCATTGCCGGAACATTCAGGAATCAAGCTGTCTATTGGGTTGGTCCTTTGGTTGGTGCTGCTGTTGCTGGTCTTCTTTATGACAATGTTTTGTTTCCTTCTCAGAATTCTGATTCTATTAGAGGGGTTTCAGATGGAACTGTTAGGGTTTAA
Protein sequence

>MS.gene025449.t1

MTGKLMPDASLNPTSLVVGAIASAFALSSVLYIAWDISGGHVNPAVTFAMAVGGHISVPTALFYWVAQLIASVIACLFLKVIVVGMHVPTYSIAEEMTGFGASILEGILTFVLVYTIYAARDTRRGQVSSTGILVIGLIAGASVLAAGPFSGGSINPACAFGSASIAGTFRNQAVYWVGPLVGAAVAGLLYDNVLFPSQNSDSIRGVSDGTVRV