Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027518.t1 | XP_003613387.1 | 83 | 323 | 25 | 1 | 1 | 293 | 126 | 448 | 3.30E-125 | 458 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027518.t1 | O48716 | 30.4 | 263 | 168 | 5 | 33 | 288 | 164 | 418 | 3.6e-27 | 123.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027518.t1 | G7K5I2 | 83.0 | 323 | 25 | 1 | 1 | 293 | 126 | 448 | 2.4e-125 | 458.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene057771 | MS.gene027518 | PPI |
| MS.gene51070 | MS.gene027518 | PPI |
| MS.gene77337 | MS.gene027518 | PPI |
| MS.gene030337 | MS.gene027518 | PPI |
| MS.gene00843 | MS.gene027518 | PPI |
| MS.gene049051 | MS.gene027518 | PPI |
| MS.gene027518 | MS.gene75024 | PPI |
| MS.gene025453 | MS.gene027518 | PPI |
| MS.gene05967 | MS.gene027518 | PPI |
| MS.gene78433 | MS.gene027518 | PPI |
| MS.gene01884 | MS.gene027518 | PPI |
| MS.gene20137 | MS.gene027518 | PPI |
| MS.gene84203 | MS.gene027518 | PPI |
| MS.gene047966 | MS.gene027518 | PPI |
| MS.gene063343 | MS.gene027518 | PPI |
| MS.gene049052 | MS.gene027518 | PPI |
| MS.gene037500 | MS.gene027518 | PPI |
| MS.gene71143 | MS.gene027518 | PPI |
| MS.gene057845 | MS.gene027518 | PPI |
| MS.gene062996 | MS.gene027518 | PPI |
| MS.gene015841 | MS.gene027518 | PPI |
| MS.gene003568 | MS.gene027518 | PPI |
| MS.gene66388 | MS.gene027518 | PPI |
| MS.gene34575 | MS.gene027518 | PPI |
| MS.gene037505 | MS.gene027518 | PPI |
| MS.gene027518 | MS.gene34510 | PPI |
| MS.gene002926 | MS.gene027518 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027518.t1 | MTR_5g035980 | 88.545 | 323 | 7 | 1 | 1 | 293 | 126 | 448 | 0.0 | 567 |
| MS.gene027518.t1 | MTR_4g106480 | 53.481 | 316 | 100 | 6 | 1 | 293 | 110 | 401 | 2.30e-110 | 325 |
| MS.gene027518.t1 | MTR_3g078380 | 53.416 | 322 | 87 | 8 | 6 | 293 | 122 | 414 | 7.21e-105 | 311 |
| MS.gene027518.t1 | MTR_8g089650 | 49.359 | 312 | 100 | 8 | 6 | 290 | 150 | 430 | 7.03e-88 | 269 |
| MS.gene027518.t1 | MTR_4g104410 | 44.118 | 306 | 112 | 7 | 8 | 288 | 92 | 363 | 2.80e-72 | 227 |
| MS.gene027518.t1 | MTR_1g021590 | 38.585 | 311 | 136 | 6 | 1 | 288 | 153 | 431 | 9.47e-64 | 207 |
| MS.gene027518.t1 | MTR_5g018520 | 37.179 | 312 | 141 | 6 | 1 | 289 | 141 | 420 | 1.51e-63 | 207 |
| MS.gene027518.t1 | MTR_4g068457 | 36.943 | 314 | 143 | 8 | 1 | 288 | 172 | 456 | 1.03e-56 | 190 |
| MS.gene027518.t1 | MTR_2g060070 | 34.951 | 309 | 143 | 11 | 1 | 288 | 110 | 381 | 1.26e-52 | 177 |
| MS.gene027518.t1 | MTR_3g110185 | 34.713 | 314 | 145 | 9 | 1 | 290 | 134 | 411 | 2.27e-40 | 149 |
| MS.gene027518.t1 | MTR_2g101410 | 31.761 | 318 | 146 | 11 | 3 | 290 | 133 | 409 | 2.62e-34 | 129 |
| MS.gene027518.t1 | MTR_8g028700 | 26.957 | 230 | 115 | 7 | 74 | 286 | 230 | 423 | 8.00e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027518.t1 | AT1G24130 | 56.230 | 313 | 97 | 5 | 1 | 293 | 123 | 415 | 1.55e-110 | 326 |
| MS.gene027518.t1 | AT5G50120 | 46.729 | 321 | 114 | 7 | 1 | 291 | 94 | 387 | 1.32e-92 | 280 |
| MS.gene027518.t1 | AT1G24530 | 48.860 | 307 | 100 | 9 | 5 | 290 | 135 | 405 | 6.95e-85 | 261 |
| MS.gene027518.t1 | AT2G26490 | 36.709 | 316 | 145 | 6 | 1 | 293 | 140 | 423 | 1.37e-63 | 207 |
| MS.gene027518.t1 | AT3G50390 | 35.987 | 314 | 144 | 7 | 1 | 288 | 142 | 424 | 1.95e-57 | 191 |
| MS.gene027518.t1 | AT4G34380 | 34.185 | 313 | 156 | 6 | 1 | 288 | 169 | 456 | 4.55e-55 | 186 |
| MS.gene027518.t1 | AT3G18950 | 35.370 | 311 | 149 | 6 | 1 | 288 | 183 | 464 | 1.63e-53 | 181 |
| MS.gene027518.t1 | AT1G49450 | 34.395 | 314 | 154 | 6 | 1 | 288 | 179 | 466 | 2.43e-53 | 181 |
| MS.gene027518.t1 | AT3G51930 | 33.956 | 321 | 146 | 10 | 1 | 288 | 107 | 394 | 1.49e-52 | 177 |
| MS.gene027518.t1 | AT1G47610 | 34.416 | 308 | 144 | 10 | 8 | 291 | 76 | 349 | 7.50e-49 | 166 |
| MS.gene027518.t1 | AT5G16750 | 23.645 | 203 | 109 | 4 | 68 | 266 | 53 | 213 | 4.53e-11 | 63.9 |
| MS.gene027518.t1 | AT4G02730 | 28.025 | 157 | 75 | 5 | 137 | 290 | 38 | 159 | 5.79e-11 | 62.8 |
Find 36 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAATTGTTGTTTCATCTTC+TGG | 0.080562 | 5.4:+25689056 | MS.gene027518:CDS |
| GGGATAGAACATTCAAAATT+TGG | 0.193532 | 5.4:+25688973 | MS.gene027518:CDS |
| TAGCCTTAGTATGCCCTCTT+AGG | 0.234642 | 5.4:-25689373 | None:intergenic |
| CTGATTATAGTGTTAGATTA+TGG | 0.250597 | 5.4:+25689441 | MS.gene027518:CDS |
| CAATTGTTGTTTCATCTTCT+GGG | 0.279540 | 5.4:+25689057 | MS.gene027518:CDS |
| TCTTCTGGGGTTGTTTATAC+AGG | 0.311488 | 5.4:+25689071 | MS.gene027518:CDS |
| AGTTTCAGATTTCAAGTGTT+TGG | 0.332190 | 5.4:+25688998 | MS.gene027518:CDS |
| TACTAAGGCTATATTATGTT+TGG | 0.339007 | 5.4:+25689385 | MS.gene027518:CDS |
| TCTCTTGCTGTTTCGAAAGA+TGG | 0.341375 | 5.4:+25688927 | MS.gene027518:CDS |
| CTCATCAAGATCACAAAATC+CGG | 0.361786 | 5.4:+25688721 | MS.gene027518:CDS |
| TTGATTGTGATATCAAAGTT+TGG | 0.373353 | 5.4:+25689621 | MS.gene027518:CDS |
| TTTGGTTGTGATGGATGATT+TGG | 0.392229 | 5.4:+25689403 | MS.gene027518:CDS |
| GTCTGGGTCTTGGTCTAGTC+TGG | 0.396268 | 5.4:-25689324 | None:intergenic |
| AAGATCACAAAATCCGGGTT+TGG | 0.420178 | 5.4:+25688727 | MS.gene027518:CDS |
| AGACCCAGACGACGAAAATA+TGG | 0.436892 | 5.4:+25689337 | MS.gene027518:CDS |
| AGATGGATTGTTTCTTTACT+CGG | 0.453556 | 5.4:+25688944 | MS.gene027518:CDS |
| AGTGTTAGATTATGGAAAAG+AGG | 0.466262 | 5.4:+25689449 | MS.gene027518:CDS |
| GTCCTAGTTGGTGCCCTAAG+AGG | 0.490038 | 5.4:+25689359 | MS.gene027518:CDS |
| TGTTTCTTTACTCGGCTTCG+TGG | 0.493077 | 5.4:+25688952 | MS.gene027518:CDS |
| TATATTATGTTTGGTTGTGA+TGG | 0.503707 | 5.4:+25689394 | MS.gene027518:CDS |
| AGCCTTAGTATGCCCTCTTA+GGG | 0.526558 | 5.4:-25689372 | None:intergenic |
| GTTTCTTTACTCGGCTTCGT+GGG | 0.536613 | 5.4:+25688953 | MS.gene027518:CDS |
| GCTTTAGCACTAAACAAAGA+TGG | 0.541869 | 5.4:+25689224 | MS.gene027518:CDS |
| ATGGATGATTTGGTGTGTAG+TGG | 0.553153 | 5.4:+25689413 | MS.gene027518:CDS |
| AATGGTGGGGTTGATGATGA+TGG | 0.555933 | 5.4:+25689569 | MS.gene027518:CDS |
| TCCTAGTTGGTGCCCTAAGA+GGG | 0.557068 | 5.4:+25689360 | MS.gene027518:CDS |
| ATAGCAGATGATTCCAAAAG+TGG | 0.557521 | 5.4:+25689542 | MS.gene027518:CDS |
| TGCCCTAAGAGGGCATACTA+AGG | 0.569818 | 5.4:+25689370 | MS.gene027518:CDS |
| GACGAAAATATGGTCCTAGT+TGG | 0.572008 | 5.4:+25689347 | MS.gene027518:CDS |
| GCCCTCTTAGGGCACCAACT+AGG | 0.579791 | 5.4:-25689361 | None:intergenic |
| AATTGTTGTTTCATCTTCTG+GGG | 0.581898 | 5.4:+25689058 | MS.gene027518:CDS |
| AGTTCTTATCTTGTTTACAG+TGG | 0.604622 | 5.4:+25689593 | MS.gene027518:CDS |
| TCATCAAGATCACAAAATCC+GGG | 0.616047 | 5.4:+25688722 | MS.gene027518:CDS |
| GGAGACATAAGAAGTATACA+TGG | 0.631337 | 5.4:+25688880 | MS.gene027518:CDS |
| ATAGTACTAATCAAGATCAG+AGG | 0.646621 | 5.4:+25688775 | MS.gene027518:CDS |
| GAGACATAAGAAGTATACAT+GGG | 0.687899 | 5.4:+25688881 | MS.gene027518:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTGATAGAAAGATAAAAATT+TGG | + | chr5.4:25689099-25689118 | MS.gene027518:intron | 20.0% |
| !!! | AAGAAAAAGCATTTTTTAGT+TGG | + | chr5.4:25689170-25689189 | MS.gene027518:intron | 20.0% |
| !!! | TGATAGATCAATTTTAGTTT+GGG | + | chr5.4:25689271-25689290 | MS.gene027518:CDS | 20.0% |
| ! | CTGATTATAGTGTTAGATTA+TGG | + | chr5.4:25689441-25689460 | MS.gene027518:CDS | 25.0% |
| ! | TAAAAAAGCTGTTAAGTGTT+TGG | + | chr5.4:25689517-25689536 | MS.gene027518:CDS | 25.0% |
| ! | TACTAAGGCTATATTATGTT+TGG | + | chr5.4:25689385-25689404 | MS.gene027518:CDS | 25.0% |
| ! | TATATTATGTTTGGTTGTGA+TGG | + | chr5.4:25689394-25689413 | MS.gene027518:CDS | 25.0% |
| ! | TGAAAAAAGTTATGCATGTT+TGG | + | chr5.4:25689478-25689497 | MS.gene027518:CDS | 25.0% |
| ! | TTGATTGTGATATCAAAGTT+TGG | + | chr5.4:25689621-25689640 | MS.gene027518:CDS | 25.0% |
| !! | TTTTTCATCGAAAAATTACG+TGG | + | chr5.4:25688851-25688870 | MS.gene027518:CDS | 25.0% |
| !!! | GTGATAGATCAATTTTAGTT+TGG | + | chr5.4:25689270-25689289 | MS.gene027518:CDS | 25.0% |
| AGTGTTAGATTATGGAAAAG+AGG | + | chr5.4:25689449-25689468 | MS.gene027518:CDS | 30.0% | |
| ATAGTACTAATCAAGATCAG+AGG | + | chr5.4:25688775-25688794 | MS.gene027518:CDS | 30.0% | |
| GAAAAACGATCATTGAATGT+TGG | - | chr5.4:25688825-25688844 | None:intergenic | 30.0% | |
| GATTCCAAAAGTGGTAAAAA+TGG | + | chr5.4:25689551-25689570 | MS.gene027518:CDS | 30.0% | |
| GGGATAGAACATTCAAAATT+TGG | + | chr5.4:25688973-25688992 | MS.gene027518:CDS | 30.0% | |
| ! | AGTTCTTATCTTGTTTACAG+TGG | + | chr5.4:25689593-25689612 | MS.gene027518:CDS | 30.0% |
| ! | AGTTTCAGATTTCAAGTGTT+TGG | + | chr5.4:25688998-25689017 | MS.gene027518:CDS | 30.0% |
| ! | GAGACATAAGAAGTATACAT+GGG | + | chr5.4:25688881-25688900 | MS.gene027518:CDS | 30.0% |
| !! | AATTGTTGTTTCATCTTCTG+GGG | + | chr5.4:25689058-25689077 | MS.gene027518:CDS | 30.0% |
| !! | AGATGGATTGTTTCTTTACT+CGG | + | chr5.4:25688944-25688963 | MS.gene027518:CDS | 30.0% |
| !! | CAATTGTTGTTTCATCTTCT+GGG | + | chr5.4:25689057-25689076 | MS.gene027518:CDS | 30.0% |
| !! | GATGGATCAGTTTTATATTC+AGG | + | chr5.4:25689242-25689261 | MS.gene027518:CDS | 30.0% |
| !!! | GCATTTTTTAGTTGGAACAT+TGG | + | chr5.4:25689178-25689197 | MS.gene027518:intron | 30.0% |
| ATAGCAGATGATTCCAAAAG+TGG | + | chr5.4:25689542-25689561 | MS.gene027518:CDS | 35.0% | |
| CAAAAGTGGTAAAAATGGTG+GGG | + | chr5.4:25689556-25689575 | MS.gene027518:CDS | 35.0% | |
| CCAAAAGTGGTAAAAATGGT+GGG | + | chr5.4:25689555-25689574 | MS.gene027518:CDS | 35.0% | |
| CTCATCAAGATCACAAAATC+CGG | + | chr5.4:25688721-25688740 | MS.gene027518:CDS | 35.0% | |
| GCTTTAGCACTAAACAAAGA+TGG | + | chr5.4:25689224-25689243 | MS.gene027518:CDS | 35.0% | |
| TCATCAAGATCACAAAATCC+GGG | + | chr5.4:25688722-25688741 | MS.gene027518:CDS | 35.0% | |
| TCCAAAAGTGGTAAAAATGG+TGG | + | chr5.4:25689554-25689573 | MS.gene027518:CDS | 35.0% | |
| ! | GGAGACATAAGAAGTATACA+TGG | + | chr5.4:25688880-25688899 | MS.gene027518:CDS | 35.0% |
| ! | TTGTTTCGATTTTCCAAACC+CGG | - | chr5.4:25688743-25688762 | None:intergenic | 35.0% |
| !! | GCAATTGTTGTTTCATCTTC+TGG | + | chr5.4:25689056-25689075 | MS.gene027518:CDS | 35.0% |
| !! | TTTGGTTGTGATGGATGATT+TGG | + | chr5.4:25689403-25689422 | MS.gene027518:CDS | 35.0% |
| AAGATCACAAAATCCGGGTT+TGG | + | chr5.4:25688727-25688746 | MS.gene027518:CDS | 40.0% | |
| CGAAAAATTACGTGGAAGTC+AGG | + | chr5.4:25688859-25688878 | MS.gene027518:CDS | 40.0% | |
| GACGAAAATATGGTCCTAGT+TGG | + | chr5.4:25689347-25689366 | MS.gene027518:CDS | 40.0% | |
| !! | ATGGATGATTTGGTGTGTAG+TGG | + | chr5.4:25689413-25689432 | MS.gene027518:CDS | 40.0% |
| !! | CCCACCATTTTTACCACTTT+TGG | - | chr5.4:25689558-25689577 | None:intergenic | 40.0% |
| !! | TCTCTTGCTGTTTCGAAAGA+TGG | + | chr5.4:25688927-25688946 | MS.gene027518:CDS | 40.0% |
| !! | TCTTCTGGGGTTGTTTATAC+AGG | + | chr5.4:25689071-25689090 | MS.gene027518:CDS | 40.0% |
| AGACCCAGACGACGAAAATA+TGG | + | chr5.4:25689337-25689356 | MS.gene027518:CDS | 45.0% | |
| AGCCTTAGTATGCCCTCTTA+GGG | - | chr5.4:25689375-25689394 | None:intergenic | 45.0% | |
| GTTTCTTTACTCGGCTTCGT+GGG | + | chr5.4:25688953-25688972 | MS.gene027518:CDS | 45.0% | |
| TGTTTCTTTACTCGGCTTCG+TGG | + | chr5.4:25688952-25688971 | MS.gene027518:CDS | 45.0% | |
| ! | AGGACCATATTTTCGTCGTC+TGG | - | chr5.4:25689344-25689363 | None:intergenic | 45.0% |
| ! | GGACCATATTTTCGTCGTCT+GGG | - | chr5.4:25689343-25689362 | None:intergenic | 45.0% |
| ! | TAGCCTTAGTATGCCCTCTT+AGG | - | chr5.4:25689376-25689395 | None:intergenic | 45.0% |
| ! | TATTTTCGTCGTCTGGGTCT+TGG | - | chr5.4:25689337-25689356 | None:intergenic | 45.0% |
| !! | AATGGTGGGGTTGATGATGA+TGG | + | chr5.4:25689569-25689588 | MS.gene027518:CDS | 45.0% |
| !! | GCATGTTTGGCTGTTTTGCA+AGG | + | chr5.4:25689491-25689510 | MS.gene027518:CDS | 45.0% |
| ! | TCCTAGTTGGTGCCCTAAGA+GGG | + | chr5.4:25689360-25689379 | MS.gene027518:CDS | 50.0% |
| ! | TGCCCTAAGAGGGCATACTA+AGG | + | chr5.4:25689370-25689389 | MS.gene027518:CDS | 50.0% |
| GTCTGGGTCTTGGTCTAGTC+TGG | - | chr5.4:25689327-25689346 | None:intergenic | 55.0% | |
| ! | GTCCTAGTTGGTGCCCTAAG+AGG | + | chr5.4:25689359-25689378 | MS.gene027518:CDS | 55.0% |
| ! | GCCCTCTTAGGGCACCAACT+AGG | - | chr5.4:25689364-25689383 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 25688690 | 25689667 | 25688690 | ID=MS.gene027518 |
| chr5.4 | mRNA | 25688690 | 25689667 | 25688690 | ID=MS.gene027518.t1;Parent=MS.gene027518 |
| chr5.4 | exon | 25688690 | 25689092 | 25688690 | ID=MS.gene027518.t1.exon1;Parent=MS.gene027518.t1 |
| chr5.4 | CDS | 25688690 | 25689092 | 25688690 | ID=cds.MS.gene027518.t1;Parent=MS.gene027518.t1 |
| chr5.4 | exon | 25689189 | 25689667 | 25689189 | ID=MS.gene027518.t1.exon2;Parent=MS.gene027518.t1 |
| chr5.4 | CDS | 25689189 | 25689667 | 25689189 | ID=cds.MS.gene027518.t1;Parent=MS.gene027518.t1 |
| Gene Sequence |
| Protein sequence |
