Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030414.t1 | XP_024629047.1 | 97.1 | 272 | 8 | 0 | 1 | 272 | 1 | 272 | 1.00E-152 | 549.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030414.t1 | Q9LXM2 | 63.4 | 265 | 92 | 3 | 9 | 270 | 17 | 279 | 1.0e-92 | 341.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030414.t1 | I3S8F5 | 97.1 | 272 | 8 | 0 | 1 | 272 | 1 | 272 | 7.3e-153 | 549.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene065708 | MS.gene030414 | PPI |
| MS.gene053225 | MS.gene030414 | PPI |
| MS.gene034816 | MS.gene030414 | PPI |
| MS.gene72978 | MS.gene030414 | PPI |
| MS.gene030414 | MS.gene007154 | PPI |
| MS.gene030414 | MS.gene72977 | PPI |
| MS.gene030414 | MS.gene41101 | PPI |
| MS.gene034815 | MS.gene030414 | PPI |
| MS.gene030414 | MS.gene025628 | PPI |
| MS.gene025629 | MS.gene030414 | PPI |
| MS.gene034250 | MS.gene030414 | PPI |
| MS.gene41100 | MS.gene030414 | PPI |
| MS.gene030414 | MS.gene065709 | PPI |
| MS.gene030414 | MS.gene065707 | PPI |
| MS.gene030414 | MS.gene41097 | PPI |
| MS.gene030414 | MS.gene000609 | PPI |
| MS.gene030414 | MS.gene21541 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030414.t1 | AT3G44260 | 63.396 | 265 | 92 | 3 | 9 | 270 | 17 | 279 | 9.94e-120 | 344 |
| MS.gene030414.t1 | AT5G22250 | 63.910 | 266 | 92 | 3 | 9 | 270 | 12 | 277 | 8.36e-119 | 341 |
| MS.gene030414.t1 | AT2G32070 | 51.493 | 268 | 125 | 5 | 4 | 270 | 5 | 268 | 1.98e-93 | 277 |
| MS.gene030414.t1 | AT5G10960 | 51.311 | 267 | 126 | 4 | 4 | 270 | 5 | 267 | 1.01e-92 | 275 |
| MS.gene030414.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 1.51e-89 | 267 |
| MS.gene030414.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 1.51e-89 | 267 |
| MS.gene030414.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 2.33e-89 | 267 |
| MS.gene030414.t1 | AT1G15920 | 48.519 | 270 | 131 | 5 | 5 | 269 | 10 | 276 | 3.93e-82 | 248 |
| MS.gene030414.t1 | AT1G15920 | 48.519 | 270 | 131 | 5 | 5 | 269 | 10 | 276 | 3.93e-82 | 248 |
| MS.gene030414.t1 | AT1G06450 | 34.799 | 273 | 154 | 9 | 1 | 268 | 1 | 254 | 1.34e-40 | 144 |
| MS.gene030414.t1 | AT1G61470 | 37.037 | 243 | 130 | 7 | 13 | 249 | 4 | 229 | 6.00e-38 | 135 |
| MS.gene030414.t1 | AT1G27820 | 35.102 | 245 | 144 | 5 | 7 | 248 | 2 | 234 | 1.93e-33 | 124 |
| MS.gene030414.t1 | AT3G44240 | 35.065 | 231 | 135 | 7 | 24 | 252 | 2 | 219 | 4.03e-33 | 121 |
| MS.gene030414.t1 | AT1G27890 | 34.728 | 239 | 141 | 5 | 13 | 248 | 3 | 229 | 1.27e-31 | 119 |
Find 62 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGGTAACCTTCCTCATTT+TGG | 0.104000 | 4.4:+6358395 | MS.gene030414:CDS |
| TTTCACAGTGCTTATGATTT+TGG | 0.125812 | 4.4:+6358623 | MS.gene030414:CDS |
| AAACACGCCGGGGTCTTATT+TGG | 0.170808 | 4.4:+6358920 | MS.gene030414:CDS |
| GCTGAATTGATGCTCACTTC+TGG | 0.258268 | 4.4:+6358566 | MS.gene030414:CDS |
| CAATCATCATCCGTCAAGTT+TGG | 0.286714 | 4.4:+6358165 | MS.gene030414:CDS |
| TCTTCAACAAATCAGTTGTT+TGG | 0.295934 | 4.4:+6358594 | MS.gene030414:CDS |
| GGACAAATAACTGCTACATA+TGG | 0.328351 | 4.4:+6358417 | MS.gene030414:CDS |
| AGCGGTTTGGCAAATTCCTT+CGG | 0.337611 | 4.4:-6358667 | None:intergenic |
| CTTAACGTGGGTCGGGTTGC+TGG | 0.360050 | 4.4:+6358806 | MS.gene030414:CDS |
| AGATGGCGGCGGTCAACTTT+GGG | 0.365096 | 4.4:-6358287 | None:intergenic |
| CTTCAACAAATCAGTTGTTT+GGG | 0.376564 | 4.4:+6358595 | MS.gene030414:CDS |
| GAATTTGCCAAACCGCTTAG+AGG | 0.386531 | 4.4:+6358673 | MS.gene030414:CDS |
| AAGATGGCGGCGGTCAACTT+TGG | 0.401395 | 4.4:-6358288 | None:intergenic |
| GCGGTTTGGCAAATTCCTTC+GGG | 0.402091 | 4.4:-6358666 | None:intergenic |
| AATCATCATCCGTCAAGTTT+GGG | 0.414340 | 4.4:+6358166 | MS.gene030414:CDS |
| AAGAAGTCCTCTAAGCGGTT+TGG | 0.423388 | 4.4:-6358680 | None:intergenic |
| CAAACTTGACGGATGATGAT+TGG | 0.450788 | 4.4:-6358164 | None:intergenic |
| AGAGCATGTCAATTGAATCT+TGG | 0.452953 | 4.4:-6358484 | None:intergenic |
| AATTAGATGCCCAAACTTGA+CGG | 0.464834 | 4.4:-6358175 | None:intergenic |
| TTCGCTTGAAGTCAATCCCT+TGG | 0.469385 | 4.4:-6358511 | None:intergenic |
| GACAAATAACTGCTACATAT+GGG | 0.475547 | 4.4:+6358418 | MS.gene030414:CDS |
| CAATTGAATCTTGGTTGTAA+AGG | 0.483444 | 4.4:-6358475 | None:intergenic |
| TGACCACTATCGTTACTTGA+AGG | 0.492206 | 4.4:+6358319 | MS.gene030414:CDS |
| TACTTATTTCATGAATAATG+AGG | 0.494803 | 4.4:+6358892 | MS.gene030414:CDS |
| TACAACACTTAACGTGGGTC+GGG | 0.508105 | 4.4:+6358799 | MS.gene030414:CDS |
| GATATTGGTTGATAACTTGA+CGG | 0.511970 | 4.4:-6358220 | None:intergenic |
| CCATGGAGATGAAAGGATAT+TGG | 0.513144 | 4.4:-6358235 | None:intergenic |
| GCTCTTAAGCTTATCCAAGT+TGG | 0.513195 | 4.4:+6358353 | MS.gene030414:CDS |
| CAGTTATTTGTCCCAAAATG+AGG | 0.514189 | 4.4:-6358407 | None:intergenic |
| CCAATATCCTTTCATCTCCA+TGG | 0.518224 | 4.4:+6358235 | MS.gene030414:CDS |
| TTCAAGCGAAATTTATGTCA+TGG | 0.518374 | 4.4:+6358524 | MS.gene030414:CDS |
| ACCAGGGAATTCAGTGTCCA+TGG | 0.528331 | 4.4:-6358252 | None:intergenic |
| GCATGCATTTAAGAAAATGA+TGG | 0.528476 | 4.4:+6358868 | MS.gene030414:CDS |
| CTACAACACTTAACGTGGGT+CGG | 0.533742 | 4.4:+6358798 | MS.gene030414:CDS |
| CAGAGAGGGTGATACCAACT+TGG | 0.540291 | 4.4:-6358367 | None:intergenic |
| ATGATATGAAACACATGATG+AGG | 0.540345 | 4.4:+6358738 | MS.gene030414:CDS |
| GCTGGAAAGTCTCATCAAGC+TGG | 0.542113 | 4.4:+6358824 | MS.gene030414:CDS |
| GCTTGAAGTCAATCCCTTGG+CGG | 0.551836 | 4.4:-6358508 | None:intergenic |
| GCGAAATTTATGTCATGGTG+TGG | 0.555209 | 4.4:+6358529 | MS.gene030414:CDS |
| TTGACATGCTCTGCCGCCAA+GGG | 0.557345 | 4.4:+6358495 | MS.gene030414:CDS |
| ATTGACATGCTCTGCCGCCA+AGG | 0.558688 | 4.4:+6358494 | MS.gene030414:CDS |
| GTTGGTATCACCCTCTCTGA+TGG | 0.573766 | 4.4:+6358371 | MS.gene030414:CDS |
| TTACCTTCAAGTAACGATAG+TGG | 0.575347 | 4.4:-6358322 | None:intergenic |
| TCCATGGACACTGAATTCCC+TGG | 0.586485 | 4.4:+6358251 | MS.gene030414:CDS |
| ATTCAAAATGAACCAGCTGA+AGG | 0.592335 | 4.4:+6358136 | None:intergenic |
| AAGTAACGATAGTGGTCAGA+GGG | 0.593594 | 4.4:-6358314 | None:intergenic |
| TATTTGTCCCAAAATGAGGA+AGG | 0.593755 | 4.4:-6358403 | None:intergenic |
| TGGTGAAGATCTTGACCCGA+AGG | 0.604460 | 4.4:+6358651 | MS.gene030414:CDS |
| TTCTAATCCAAATAAGACCC+CGG | 0.610017 | 4.4:-6358927 | None:intergenic |
| TCGTTACTTGAAGGTAAATG+TGG | 0.610719 | 4.4:+6358328 | MS.gene030414:CDS |
| CAGAGGGTTTAAGATGGCGG+CGG | 0.622753 | 4.4:-6358298 | None:intergenic |
| AGTCGCTACAACACTTAACG+TGG | 0.625698 | 4.4:+6358793 | MS.gene030414:CDS |
| AGTGGTCAGAGGGTTTAAGA+TGG | 0.629355 | 4.4:-6358304 | None:intergenic |
| CAAGTAACGATAGTGGTCAG+AGG | 0.631701 | 4.4:-6358315 | None:intergenic |
| ATCACCCTCTCTGATGGTAA+CGG | 0.635418 | 4.4:+6358377 | MS.gene030414:CDS |
| GGTCAGAGGGTTTAAGATGG+CGG | 0.648588 | 4.4:-6358301 | None:intergenic |
| TCAGTGTCCATGGAGATGAA+AGG | 0.659721 | 4.4:-6358242 | None:intergenic |
| GATCAGATAGTTTGTTGACG+TGG | 0.660381 | 4.4:+6358846 | MS.gene030414:CDS |
| TATTTAAGAAGTCCTCTAAG+CGG | 0.667001 | 4.4:-6358685 | None:intergenic |
| GTTACCGTTACCATCAGAGA+GGG | 0.698499 | 4.4:-6358381 | None:intergenic |
| GTCGCTACAACACTTAACGT+GGG | 0.701411 | 4.4:+6358794 | MS.gene030414:CDS |
| GGTTACCGTTACCATCAGAG+AGG | 0.730463 | 4.4:-6358382 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATTTTAACAATATTGTT+TGG | + | chr4.4:6358704-6358723 | MS.gene030414:CDS | 10.0% |
| !! | TACTTATTTCATGAATAATG+AGG | + | chr4.4:6358892-6358911 | MS.gene030414:CDS | 20.0% |
| !! | TGCTTATGATTTTGGATATT+TGG | + | chr4.4:6358631-6358650 | MS.gene030414:CDS | 25.0% |
| ATGATATGAAACACATGATG+AGG | + | chr4.4:6358738-6358757 | MS.gene030414:CDS | 30.0% | |
| CAATTGAATCTTGGTTGTAA+AGG | - | chr4.4:6358478-6358497 | None:intergenic | 30.0% | |
| CTTCAACAAATCAGTTGTTT+GGG | + | chr4.4:6358595-6358614 | MS.gene030414:CDS | 30.0% | |
| GACAAATAACTGCTACATAT+GGG | + | chr4.4:6358418-6358437 | MS.gene030414:CDS | 30.0% | |
| GCATGCATTTAAGAAAATGA+TGG | + | chr4.4:6358868-6358887 | MS.gene030414:CDS | 30.0% | |
| TATTTAAGAAGTCCTCTAAG+CGG | - | chr4.4:6358688-6358707 | None:intergenic | 30.0% | |
| TCTTCAACAAATCAGTTGTT+TGG | + | chr4.4:6358594-6358613 | MS.gene030414:CDS | 30.0% | |
| TTCAAGCGAAATTTATGTCA+TGG | + | chr4.4:6358524-6358543 | MS.gene030414:CDS | 30.0% | |
| ! | TTTCACAGTGCTTATGATTT+TGG | + | chr4.4:6358623-6358642 | MS.gene030414:CDS | 30.0% |
| !! | GATATTGGTTGATAACTTGA+CGG | - | chr4.4:6358223-6358242 | None:intergenic | 30.0% |
| AATCATCATCCGTCAAGTTT+GGG | + | chr4.4:6358166-6358185 | MS.gene030414:CDS | 35.0% | |
| AATTAGATGCCCAAACTTGA+CGG | - | chr4.4:6358178-6358197 | None:intergenic | 35.0% | |
| AGAGCATGTCAATTGAATCT+TGG | - | chr4.4:6358487-6358506 | None:intergenic | 35.0% | |
| CAGTTATTTGTCCCAAAATG+AGG | - | chr4.4:6358410-6358429 | None:intergenic | 35.0% | |
| GGACAAATAACTGCTACATA+TGG | + | chr4.4:6358417-6358436 | MS.gene030414:CDS | 35.0% | |
| TATTTGTCCCAAAATGAGGA+AGG | - | chr4.4:6358406-6358425 | None:intergenic | 35.0% | |
| TCGTTACTTGAAGGTAAATG+TGG | + | chr4.4:6358328-6358347 | MS.gene030414:CDS | 35.0% | |
| TTACCTTCAAGTAACGATAG+TGG | - | chr4.4:6358325-6358344 | None:intergenic | 35.0% | |
| TTCTAATCCAAATAAGACCC+CGG | - | chr4.4:6358930-6358949 | None:intergenic | 35.0% | |
| CAATCATCATCCGTCAAGTT+TGG | + | chr4.4:6358165-6358184 | MS.gene030414:CDS | 40.0% | |
| CCAATATCCTTTCATCTCCA+TGG | + | chr4.4:6358235-6358254 | MS.gene030414:CDS | 40.0% | |
| CCATGGAGATGAAAGGATAT+TGG | - | chr4.4:6358238-6358257 | None:intergenic | 40.0% | |
| GCGAAATTTATGTCATGGTG+TGG | + | chr4.4:6358529-6358548 | MS.gene030414:CDS | 40.0% | |
| GCTCTTAAGCTTATCCAAGT+TGG | + | chr4.4:6358353-6358372 | MS.gene030414:CDS | 40.0% | |
| TGACCACTATCGTTACTTGA+AGG | + | chr4.4:6358319-6358338 | MS.gene030414:CDS | 40.0% | |
| TGGGTGAAAAAATCACACCA+GGG | - | chr4.4:6358271-6358290 | None:intergenic | 40.0% | |
| TTGGGTGAAAAAATCACACC+AGG | - | chr4.4:6358272-6358291 | None:intergenic | 40.0% | |
| ! | AACGGTAACCTTCCTCATTT+TGG | + | chr4.4:6358395-6358414 | MS.gene030414:CDS | 40.0% |
| ! | AAGTAACGATAGTGGTCAGA+GGG | - | chr4.4:6358317-6358336 | None:intergenic | 40.0% |
| ! | ACGGTAACCTTCCTCATTTT+GGG | + | chr4.4:6358396-6358415 | MS.gene030414:CDS | 40.0% |
| ! | AGGTTTTGCAACGCTTTGTA+CGG | + | chr4.4:6358758-6358777 | MS.gene030414:CDS | 40.0% |
| ! | CAAACTTGACGGATGATGAT+TGG | - | chr4.4:6358167-6358186 | None:intergenic | 40.0% |
| !! | GATCAGATAGTTTGTTGACG+TGG | + | chr4.4:6358846-6358865 | MS.gene030414:CDS | 40.0% |
| AAGAAGTCCTCTAAGCGGTT+TGG | - | chr4.4:6358683-6358702 | None:intergenic | 45.0% | |
| AATGAGGCTCAAAAACACGC+CGG | + | chr4.4:6358908-6358927 | MS.gene030414:CDS | 45.0% | |
| AGTCGCTACAACACTTAACG+TGG | + | chr4.4:6358793-6358812 | MS.gene030414:CDS | 45.0% | |
| AGTGGTCAGAGGGTTTAAGA+TGG | - | chr4.4:6358307-6358326 | None:intergenic | 45.0% | |
| ATCACCCTCTCTGATGGTAA+CGG | + | chr4.4:6358377-6358396 | MS.gene030414:CDS | 45.0% | |
| CTACAACACTTAACGTGGGT+CGG | + | chr4.4:6358798-6358817 | MS.gene030414:CDS | 45.0% | |
| GAATTTGCCAAACCGCTTAG+AGG | + | chr4.4:6358673-6358692 | MS.gene030414:CDS | 45.0% | |
| GCTGAATTGATGCTCACTTC+TGG | + | chr4.4:6358566-6358585 | MS.gene030414:CDS | 45.0% | |
| GTCGCTACAACACTTAACGT+GGG | + | chr4.4:6358794-6358813 | MS.gene030414:CDS | 45.0% | |
| GTTACCGTTACCATCAGAGA+GGG | - | chr4.4:6358384-6358403 | None:intergenic | 45.0% | |
| TACAACACTTAACGTGGGTC+GGG | + | chr4.4:6358799-6358818 | MS.gene030414:CDS | 45.0% | |
| TCAGTGTCCATGGAGATGAA+AGG | - | chr4.4:6358245-6358264 | None:intergenic | 45.0% | |
| TTCGCTTGAAGTCAATCCCT+TGG | - | chr4.4:6358514-6358533 | None:intergenic | 45.0% | |
| ! | AGCGGTTTGGCAAATTCCTT+CGG | - | chr4.4:6358670-6358689 | None:intergenic | 45.0% |
| ! | CAAGTAACGATAGTGGTCAG+AGG | - | chr4.4:6358318-6358337 | None:intergenic | 45.0% |
| ! | TTTTGCAACGCTTTGTACGG+TGG | + | chr4.4:6358761-6358780 | MS.gene030414:CDS | 45.0% |
| !!! | TGATTGGCTTTTCCTTCAGC+TGG | - | chr4.4:6358151-6358170 | None:intergenic | 45.0% |
| ACCAGGGAATTCAGTGTCCA+TGG | - | chr4.4:6358255-6358274 | None:intergenic | 50.0% | |
| ATGAGGCTCAAAAACACGCC+GGG | + | chr4.4:6358909-6358928 | MS.gene030414:CDS | 50.0% | |
| CAGAGAGGGTGATACCAACT+TGG | - | chr4.4:6358370-6358389 | None:intergenic | 50.0% | |
| GCTGGAAAGTCTCATCAAGC+TGG | + | chr4.4:6358824-6358843 | MS.gene030414:CDS | 50.0% | |
| GCTTGAAGTCAATCCCTTGG+CGG | - | chr4.4:6358511-6358530 | None:intergenic | 50.0% | |
| GGTTACCGTTACCATCAGAG+AGG | - | chr4.4:6358385-6358404 | None:intergenic | 50.0% | |
| GTTGGTATCACCCTCTCTGA+TGG | + | chr4.4:6358371-6358390 | MS.gene030414:CDS | 50.0% | |
| TCCATGGACACTGAATTCCC+TGG | + | chr4.4:6358251-6358270 | MS.gene030414:CDS | 50.0% | |
| TGGTGAAGATCTTGACCCGA+AGG | + | chr4.4:6358651-6358670 | MS.gene030414:CDS | 50.0% | |
| ! | GCGGTTTGGCAAATTCCTTC+GGG | - | chr4.4:6358669-6358688 | None:intergenic | 50.0% |
| ! | GGTCAGAGGGTTTAAGATGG+CGG | - | chr4.4:6358304-6358323 | None:intergenic | 50.0% |
| !! | AAACACGCCGGGGTCTTATT+TGG | + | chr4.4:6358920-6358939 | MS.gene030414:CDS | 50.0% |
| ATTGACATGCTCTGCCGCCA+AGG | + | chr4.4:6358494-6358513 | MS.gene030414:CDS | 55.0% | |
| TGAGGCTCAAAAACACGCCG+GGG | + | chr4.4:6358910-6358929 | MS.gene030414:CDS | 55.0% | |
| TTGACATGCTCTGCCGCCAA+GGG | + | chr4.4:6358495-6358514 | MS.gene030414:CDS | 55.0% | |
| ! | AAGATGGCGGCGGTCAACTT+TGG | - | chr4.4:6358291-6358310 | None:intergenic | 55.0% |
| ! | AGATGGCGGCGGTCAACTTT+GGG | - | chr4.4:6358290-6358309 | None:intergenic | 55.0% |
| ! | CAGAGGGTTTAAGATGGCGG+CGG | - | chr4.4:6358301-6358320 | None:intergenic | 55.0% |
| ! | CTTAACGTGGGTCGGGTTGC+TGG | + | chr4.4:6358806-6358825 | MS.gene030414:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 6358143 | 6358961 | 6358143 | ID=MS.gene030414 |
| chr4.4 | mRNA | 6358143 | 6358961 | 6358143 | ID=MS.gene030414.t1;Parent=MS.gene030414 |
| chr4.4 | exon | 6358143 | 6358961 | 6358143 | ID=MS.gene030414.t1.exon1;Parent=MS.gene030414.t1 |
| chr4.4 | CDS | 6358143 | 6358961 | 6358143 | ID=cds.MS.gene030414.t1;Parent=MS.gene030414.t1 |
| Gene Sequence |
| Protein sequence |
