Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030415.t1 | XP_024629048.1 | 96 | 272 | 11 | 0 | 1 | 272 | 1 | 272 | 4.70E-150 | 540.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030415.t1 | Q9FMS6 | 64.3 | 266 | 91 | 3 | 9 | 270 | 12 | 277 | 1.5e-91 | 337.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030415.t1 | I3SR57 | 96.0 | 272 | 11 | 0 | 1 | 272 | 1 | 272 | 3.4e-150 | 540.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene030415 | MS.gene007154 | PPI |
| MS.gene030415 | MS.gene41101 | PPI |
| MS.gene053225 | MS.gene030415 | PPI |
| MS.gene030415 | MS.gene065708 | PPI |
| MS.gene034815 | MS.gene030415 | PPI |
| MS.gene030415 | MS.gene065709 | PPI |
| MS.gene030415 | MS.gene72977 | PPI |
| MS.gene025629 | MS.gene030415 | PPI |
| MS.gene034250 | MS.gene030415 | PPI |
| MS.gene030415 | MS.gene025628 | PPI |
| MS.gene030415 | MS.gene72978 | PPI |
| MS.gene030415 | MS.gene41100 | PPI |
| MS.gene030415 | MS.gene065707 | PPI |
| MS.gene030415 | MS.gene000609 | PPI |
| MS.gene030415 | MS.gene034816 | PPI |
| MS.gene030415 | MS.gene21541 | PPI |
| MS.gene030415 | MS.gene41097 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030415.t1 | AT5G22250 | 64.286 | 266 | 91 | 3 | 9 | 270 | 12 | 277 | 2.80e-119 | 342 |
| MS.gene030415.t1 | AT3G44260 | 61.509 | 265 | 97 | 3 | 9 | 270 | 17 | 279 | 6.17e-117 | 337 |
| MS.gene030415.t1 | AT2G32070 | 50.746 | 268 | 127 | 5 | 4 | 270 | 5 | 268 | 3.56e-92 | 274 |
| MS.gene030415.t1 | AT5G10960 | 50.936 | 267 | 127 | 4 | 4 | 270 | 5 | 267 | 1.35e-91 | 272 |
| MS.gene030415.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 9.44e-89 | 265 |
| MS.gene030415.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 9.44e-89 | 265 |
| MS.gene030415.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 1.10e-88 | 265 |
| MS.gene030415.t1 | AT1G15920 | 47.407 | 270 | 134 | 5 | 5 | 269 | 10 | 276 | 5.97e-81 | 245 |
| MS.gene030415.t1 | AT1G15920 | 47.407 | 270 | 134 | 5 | 5 | 269 | 10 | 276 | 5.97e-81 | 245 |
| MS.gene030415.t1 | AT1G61470 | 37.037 | 243 | 130 | 7 | 13 | 249 | 4 | 229 | 1.11e-38 | 137 |
| MS.gene030415.t1 | AT1G06450 | 33.700 | 273 | 157 | 9 | 1 | 268 | 1 | 254 | 1.21e-38 | 139 |
| MS.gene030415.t1 | AT1G27820 | 35.510 | 245 | 143 | 5 | 7 | 248 | 2 | 234 | 4.04e-34 | 125 |
| MS.gene030415.t1 | AT3G44240 | 35.683 | 227 | 131 | 7 | 25 | 249 | 3 | 216 | 7.46e-33 | 120 |
| MS.gene030415.t1 | AT1G27890 | 35.565 | 239 | 139 | 5 | 13 | 248 | 3 | 229 | 9.74e-33 | 122 |
Find 64 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCACAGCGCTTATGATTT+TGG | 0.104590 | 4.4:+6361608 | MS.gene030415:CDS |
| AATGGCAACCTTCCTCATTT+TGG | 0.121265 | 4.4:+6361380 | MS.gene030415:CDS |
| ATCAAATTCAACCTCTAAAT+TGG | 0.207989 | 4.4:-6361177 | None:intergenic |
| AAACACGCTGGGGTACTATT+TGG | 0.216392 | 4.4:+6361905 | MS.gene030415:CDS |
| GCTGAATTGATGCTCACTTC+TGG | 0.258268 | 4.4:+6361551 | MS.gene030415:CDS |
| CAATCATCATCCGTCAAGTT+TGG | 0.286714 | 4.4:+6361150 | MS.gene030415:CDS |
| TCTTCAACAAATCAGTTGTT+TGG | 0.295934 | 4.4:+6361579 | MS.gene030415:CDS |
| AACGGTTCGGCAGATTCCTT+CGG | 0.352418 | 4.4:-6361652 | None:intergenic |
| CTTAACGTGGGTCGGGTTGC+TGG | 0.360050 | 4.4:+6361791 | MS.gene030415:CDS |
| AGATGACGGCGGTCAACTTT+GGG | 0.368577 | 4.4:-6361272 | None:intergenic |
| CTTCAACAAATCAGTTGTTT+GGG | 0.376564 | 4.4:+6361580 | MS.gene030415:CDS |
| TCCATGGATACTGAATTTCC+AGG | 0.393192 | 4.4:+6361236 | MS.gene030415:CDS |
| GGACAAATAGCCGCTACATA+TGG | 0.395638 | 4.4:+6361402 | MS.gene030415:CDS |
| ACGGTTCGGCAGATTCCTTC+GGG | 0.395658 | 4.4:-6361651 | None:intergenic |
| AATCATCATCCGTCAAGTTT+GGG | 0.414340 | 4.4:+6361151 | MS.gene030415:CDS |
| AAGATGACGGCGGTCAACTT+TGG | 0.418710 | 4.4:-6361273 | None:intergenic |
| GAATCTGCCGAACCGTTTAG+AGG | 0.420121 | 4.4:+6361658 | MS.gene030415:CDS |
| AGTTTGGGCATCCAATTTAG+AGG | 0.424325 | 4.4:+6361166 | MS.gene030415:CDS |
| GGTTGCCATTACCATCAGAT+AGG | 0.447285 | 4.4:-6361367 | None:intergenic |
| CAAACTTGACGGATGATGAT+TGG | 0.450788 | 4.4:-6361149 | None:intergenic |
| TACTCATTTCTTGAATAATG+AGG | 0.477666 | 4.4:+6361877 | MS.gene030415:CDS |
| TGACCACTATCGTTACTTGA+AGG | 0.492905 | 4.4:+6361304 | MS.gene030415:CDS |
| CCCTGGAAATTCAGTATCCA+TGG | 0.496084 | 4.4:-6361237 | None:intergenic |
| GACAAATAGCCGCTACATAT+GGG | 0.500452 | 4.4:+6361403 | MS.gene030415:CDS |
| CAATTGAGCCTTGGTTGTAA+AGG | 0.504025 | 4.4:-6361460 | None:intergenic |
| TGCGCTTGAAGTCAATCCCT+TGG | 0.505546 | 4.4:-6361496 | None:intergenic |
| TCAATATCCTTTCATCTCCA+TGG | 0.506697 | 4.4:+6361220 | MS.gene030415:CDS |
| TACAACACTTAACGTGGGTC+GGG | 0.508105 | 4.4:+6361784 | MS.gene030415:CDS |
| AATTGGATGCCCAAACTTGA+CGG | 0.510297 | 4.4:-6361160 | None:intergenic |
| AGGTTCTGCAACGCTTTGTA+CGG | 0.515634 | 4.4:+6361743 | MS.gene030415:CDS |
| TTCAAGCGCAATTTATCTCA+TGG | 0.520248 | 4.4:+6361509 | MS.gene030415:CDS |
| CAGATAGGGTGATACCAACT+TGG | 0.523809 | 4.4:-6361352 | None:intergenic |
| GATATTGATTGACAACTTGA+CGG | 0.523978 | 4.4:-6361205 | None:intergenic |
| GCATGCATTTAAGAAAATGA+TGG | 0.528476 | 4.4:+6361853 | MS.gene030415:CDS |
| TTGGGTGAATAAATTACCCC+TGG | 0.529877 | 4.4:-6361254 | None:intergenic |
| CTACAACACTTAACGTGGGT+CGG | 0.533742 | 4.4:+6361783 | MS.gene030415:CDS |
| CGGCTATTTGTCCCAAAATG+AGG | 0.540450 | 4.4:-6361392 | None:intergenic |
| GCTGGAAAGTCTCATCAAGC+TGG | 0.542113 | 4.4:+6361809 | MS.gene030415:CDS |
| ATTGACATGCTCTGCCGCCA+AGG | 0.544877 | 4.4:+6361479 | MS.gene030415:CDS |
| AGAGCATGTCAATTGAGCCT+TGG | 0.548111 | 4.4:-6361469 | None:intergenic |
| ATCACCCTATCTGATGGTAA+TGG | 0.551182 | 4.4:+6361362 | MS.gene030415:CDS |
| TTGACATGCTCTGCCGCCAA+GGG | 0.557345 | 4.4:+6361480 | MS.gene030415:CDS |
| GCTTGAAGTCAATCCCTTGG+CGG | 0.562753 | 4.4:-6361493 | None:intergenic |
| CCATGGATACTGAATTTCCA+GGG | 0.565112 | 4.4:+6361237 | MS.gene030415:CDS |
| GCTCTTAAACTCATCCAAGT+TGG | 0.568904 | 4.4:+6361338 | MS.gene030415:CDS |
| ATGATATGAAACACATGATG+AGG | 0.569362 | 4.4:+6361723 | MS.gene030415:CDS |
| TTCTGCAACGCTTTGTACGG+TGG | 0.584961 | 4.4:+6361746 | MS.gene030415:CDS |
| ATTCAAAATGAACCAGCTGA+AGG | 0.587598 | 4.4:+6361121 | None:intergenic |
| GGTCAGAGGGTTTAAGATGA+CGG | 0.592880 | 4.4:-6361286 | None:intergenic |
| AAGTAACGATAGTGGTCAGA+GGG | 0.593594 | 4.4:-6361299 | None:intergenic |
| TAGTGAAGATATTGACCCGA+AGG | 0.595020 | 4.4:+6361636 | MS.gene030415:CDS |
| GTTGGTATCACCCTATCTGA+TGG | 0.596348 | 4.4:+6361356 | MS.gene030415:CDS |
| TCAGTATCCATGGAGATGAA+AGG | 0.598752 | 4.4:-6361227 | None:intergenic |
| GTTGCCATTACCATCAGATA+GGG | 0.603208 | 4.4:-6361366 | None:intergenic |
| TGACCTTCAAGTAACGATAG+TGG | 0.622378 | 4.4:-6361307 | None:intergenic |
| TATTTGTCCCAAAATGAGGA+AGG | 0.623706 | 4.4:-6361388 | None:intergenic |
| AGTCGCTACAACACTTAACG+TGG | 0.625698 | 4.4:+6361778 | MS.gene030415:CDS |
| CAAGTAACGATAGTGGTCAG+AGG | 0.631701 | 4.4:-6361300 | None:intergenic |
| GAGCATGACCTTTACAACCA+AGG | 0.646186 | 4.4:+6361452 | MS.gene030415:CDS |
| AGTTGAATTCCCATATGTAG+CGG | 0.649610 | 4.4:-6361412 | None:intergenic |
| GATCAGATAGTTTGTTGACG+TGG | 0.660381 | 4.4:+6361831 | MS.gene030415:CDS |
| CATGGATACTGAATTTCCAG+GGG | 0.700170 | 4.4:+6361238 | MS.gene030415:CDS |
| GTCGCTACAACACTTAACGT+GGG | 0.701411 | 4.4:+6361779 | MS.gene030415:CDS |
| CAGAGGGTTTAAGATGACGG+CGG | 0.736962 | 4.4:-6361283 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTTAAAAAGTCCTCTAAA+CGG | - | chr4.4:6361673-6361692 | None:intergenic | 20.0% |
| ! | ATCAAATTCAACCTCTAAAT+TGG | - | chr4.4:6361180-6361199 | None:intergenic | 25.0% |
| ! | TACTCATTTCTTGAATAATG+AGG | + | chr4.4:6361877-6361896 | MS.gene030415:CDS | 25.0% |
| ATGATATGAAACACATGATG+AGG | + | chr4.4:6361723-6361742 | MS.gene030415:CDS | 30.0% | |
| CTTCAACAAATCAGTTGTTT+GGG | + | chr4.4:6361580-6361599 | MS.gene030415:CDS | 30.0% | |
| GCATGCATTTAAGAAAATGA+TGG | + | chr4.4:6361853-6361872 | MS.gene030415:CDS | 30.0% | |
| TCTTCAACAAATCAGTTGTT+TGG | + | chr4.4:6361579-6361598 | MS.gene030415:CDS | 30.0% | |
| ! | GATATTGATTGACAACTTGA+CGG | - | chr4.4:6361208-6361227 | None:intergenic | 30.0% |
| AAAAAGTCCTCTAAACGGTT+CGG | - | chr4.4:6361668-6361687 | None:intergenic | 35.0% | |
| AATCATCATCCGTCAAGTTT+GGG | + | chr4.4:6361151-6361170 | MS.gene030415:CDS | 35.0% | |
| AGTTGAATTCCCATATGTAG+CGG | - | chr4.4:6361415-6361434 | None:intergenic | 35.0% | |
| TATTTGTCCCAAAATGAGGA+AGG | - | chr4.4:6361391-6361410 | None:intergenic | 35.0% | |
| TCAATATCCTTTCATCTCCA+TGG | + | chr4.4:6361220-6361239 | MS.gene030415:CDS | 35.0% | |
| TTCAAGCGCAATTTATCTCA+TGG | + | chr4.4:6361509-6361528 | MS.gene030415:CDS | 35.0% | |
| ! | TTCACAGCGCTTATGATTTT+GGG | + | chr4.4:6361609-6361628 | MS.gene030415:CDS | 35.0% |
| ! | TTTCACAGCGCTTATGATTT+TGG | + | chr4.4:6361608-6361627 | MS.gene030415:CDS | 35.0% |
| AATTGGATGCCCAAACTTGA+CGG | - | chr4.4:6361163-6361182 | None:intergenic | 40.0% | |
| AGTTTGGGCATCCAATTTAG+AGG | + | chr4.4:6361166-6361185 | MS.gene030415:CDS | 40.0% | |
| ATCACCCTATCTGATGGTAA+TGG | + | chr4.4:6361362-6361381 | MS.gene030415:CDS | 40.0% | |
| CAATCATCATCCGTCAAGTT+TGG | + | chr4.4:6361150-6361169 | MS.gene030415:CDS | 40.0% | |
| CAATTGAGCCTTGGTTGTAA+AGG | - | chr4.4:6361463-6361482 | None:intergenic | 40.0% | |
| CATGGATACTGAATTTCCAG+GGG | + | chr4.4:6361238-6361257 | MS.gene030415:CDS | 40.0% | |
| CCATGGATACTGAATTTCCA+GGG | + | chr4.4:6361237-6361256 | MS.gene030415:CDS | 40.0% | |
| GACAAATAGCCGCTACATAT+GGG | + | chr4.4:6361403-6361422 | MS.gene030415:CDS | 40.0% | |
| GCTCTTAAACTCATCCAAGT+TGG | + | chr4.4:6361338-6361357 | MS.gene030415:CDS | 40.0% | |
| GTTGCCATTACCATCAGATA+GGG | - | chr4.4:6361369-6361388 | None:intergenic | 40.0% | |
| TAGTGAAGATATTGACCCGA+AGG | + | chr4.4:6361636-6361655 | MS.gene030415:CDS | 40.0% | |
| TCAGTATCCATGGAGATGAA+AGG | - | chr4.4:6361230-6361249 | None:intergenic | 40.0% | |
| TCCATGGATACTGAATTTCC+AGG | + | chr4.4:6361236-6361255 | MS.gene030415:CDS | 40.0% | |
| TGACCACTATCGTTACTTGA+AGG | + | chr4.4:6361304-6361323 | MS.gene030415:CDS | 40.0% | |
| TGACCTTCAAGTAACGATAG+TGG | - | chr4.4:6361310-6361329 | None:intergenic | 40.0% | |
| TTGGGTGAATAAATTACCCC+TGG | - | chr4.4:6361257-6361276 | None:intergenic | 40.0% | |
| ! | AAGTAACGATAGTGGTCAGA+GGG | - | chr4.4:6361302-6361321 | None:intergenic | 40.0% |
| ! | AATGGCAACCTTCCTCATTT+TGG | + | chr4.4:6361380-6361399 | MS.gene030415:CDS | 40.0% |
| ! | ATGGCAACCTTCCTCATTTT+GGG | + | chr4.4:6361381-6361400 | MS.gene030415:CDS | 40.0% |
| ! | CAAACTTGACGGATGATGAT+TGG | - | chr4.4:6361152-6361171 | None:intergenic | 40.0% |
| !! | GATCAGATAGTTTGTTGACG+TGG | + | chr4.4:6361831-6361850 | MS.gene030415:CDS | 40.0% |
| AATGAGGCTCAAAAACACGC+TGG | + | chr4.4:6361893-6361912 | MS.gene030415:CDS | 45.0% | |
| AGAGCATGTCAATTGAGCCT+TGG | - | chr4.4:6361472-6361491 | None:intergenic | 45.0% | |
| AGGTTCTGCAACGCTTTGTA+CGG | + | chr4.4:6361743-6361762 | MS.gene030415:CDS | 45.0% | |
| AGTCGCTACAACACTTAACG+TGG | + | chr4.4:6361778-6361797 | MS.gene030415:CDS | 45.0% | |
| ATGAGGCTCAAAAACACGCT+GGG | + | chr4.4:6361894-6361913 | MS.gene030415:CDS | 45.0% | |
| CAGATAGGGTGATACCAACT+TGG | - | chr4.4:6361355-6361374 | None:intergenic | 45.0% | |
| CCCTGGAAATTCAGTATCCA+TGG | - | chr4.4:6361240-6361259 | None:intergenic | 45.0% | |
| CGGCTATTTGTCCCAAAATG+AGG | - | chr4.4:6361395-6361414 | None:intergenic | 45.0% | |
| CTACAACACTTAACGTGGGT+CGG | + | chr4.4:6361783-6361802 | MS.gene030415:CDS | 45.0% | |
| GAGCATGACCTTTACAACCA+AGG | + | chr4.4:6361452-6361471 | MS.gene030415:CDS | 45.0% | |
| GCTGAATTGATGCTCACTTC+TGG | + | chr4.4:6361551-6361570 | MS.gene030415:CDS | 45.0% | |
| GGACAAATAGCCGCTACATA+TGG | + | chr4.4:6361402-6361421 | MS.gene030415:CDS | 45.0% | |
| GGTTGCCATTACCATCAGAT+AGG | - | chr4.4:6361370-6361389 | None:intergenic | 45.0% | |
| GTCGCTACAACACTTAACGT+GGG | + | chr4.4:6361779-6361798 | MS.gene030415:CDS | 45.0% | |
| GTTGGTATCACCCTATCTGA+TGG | + | chr4.4:6361356-6361375 | MS.gene030415:CDS | 45.0% | |
| TACAACACTTAACGTGGGTC+GGG | + | chr4.4:6361784-6361803 | MS.gene030415:CDS | 45.0% | |
| ! | AAACACGCTGGGGTACTATT+TGG | + | chr4.4:6361905-6361924 | MS.gene030415:CDS | 45.0% |
| ! | CAAGTAACGATAGTGGTCAG+AGG | - | chr4.4:6361303-6361322 | None:intergenic | 45.0% |
| ! | GGTCAGAGGGTTTAAGATGA+CGG | - | chr4.4:6361289-6361308 | None:intergenic | 45.0% |
| !!! | TGATTGGCTTTTCCTTCAGC+TGG | - | chr4.4:6361136-6361155 | None:intergenic | 45.0% |
| !!! | AATATTTTAACAATATTATT+TGG | + | chr4.4:6361689-6361708 | MS.gene030415:CDS | 5.0% |
| GAATCTGCCGAACCGTTTAG+AGG | + | chr4.4:6361658-6361677 | MS.gene030415:CDS | 50.0% | |
| GCTGGAAAGTCTCATCAAGC+TGG | + | chr4.4:6361809-6361828 | MS.gene030415:CDS | 50.0% | |
| GCTTGAAGTCAATCCCTTGG+CGG | - | chr4.4:6361496-6361515 | None:intergenic | 50.0% | |
| TGAGGCTCAAAAACACGCTG+GGG | + | chr4.4:6361895-6361914 | MS.gene030415:CDS | 50.0% | |
| TGCGCTTGAAGTCAATCCCT+TGG | - | chr4.4:6361499-6361518 | None:intergenic | 50.0% | |
| TTCTGCAACGCTTTGTACGG+TGG | + | chr4.4:6361746-6361765 | MS.gene030415:CDS | 50.0% | |
| ! | AACGGTTCGGCAGATTCCTT+CGG | - | chr4.4:6361655-6361674 | None:intergenic | 50.0% |
| ! | AAGATGACGGCGGTCAACTT+TGG | - | chr4.4:6361276-6361295 | None:intergenic | 50.0% |
| ! | AGATGACGGCGGTCAACTTT+GGG | - | chr4.4:6361275-6361294 | None:intergenic | 50.0% |
| ! | CAGAGGGTTTAAGATGACGG+CGG | - | chr4.4:6361286-6361305 | None:intergenic | 50.0% |
| ATTGACATGCTCTGCCGCCA+AGG | + | chr4.4:6361479-6361498 | MS.gene030415:CDS | 55.0% | |
| TTGACATGCTCTGCCGCCAA+GGG | + | chr4.4:6361480-6361499 | MS.gene030415:CDS | 55.0% | |
| ! | ACGGTTCGGCAGATTCCTTC+GGG | - | chr4.4:6361654-6361673 | None:intergenic | 55.0% |
| ! | CTTAACGTGGGTCGGGTTGC+TGG | + | chr4.4:6361791-6361810 | MS.gene030415:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 6361128 | 6361946 | 6361128 | ID=MS.gene030415 |
| chr4.4 | mRNA | 6361128 | 6361946 | 6361128 | ID=MS.gene030415.t1;Parent=MS.gene030415 |
| chr4.4 | exon | 6361128 | 6361946 | 6361128 | ID=MS.gene030415.t1.exon1;Parent=MS.gene030415.t1 |
| chr4.4 | CDS | 6361128 | 6361946 | 6361128 | ID=cds.MS.gene030415.t1;Parent=MS.gene030415.t1 |
| Gene Sequence |
| Protein sequence |
