AlfalfaGEDB | MS.gene032699

M. sativa cultivar XinJiangDaYe / MS.gene032699

KEGG: -
GO: GO:0003674 GO:0005488 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0030001 GO:0043167 GO:0043169 GO:0046872 GO:0051179 GO:0051234
PFAM: PF00403.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032699.t1	XP_013450688.1	98.1	155	3	0	1	155	1	155	7.20E-79	303.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032699.t1	Q9LF57	65.4	156	46	5	1	155	1	149	2.5e-48	193.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032699.t1	A0A072UG06	98.1	155	3	0	1	155	1	155	5.2e-79	303.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene032699	MS.gene86494	PPI
MS.gene83137	MS.gene032699	PPI
MS.gene032699	MS.gene72709	PPI
MS.gene032699	MS.gene82488	PPI
MS.gene032699	MS.gene59148	PPI
MS.gene62995	MS.gene032699	PPI
MS.gene032699	MS.gene59150	PPI
MS.gene88333	MS.gene032699	PPI
MS.gene83138	MS.gene032699	PPI
MS.gene62994	MS.gene032699	PPI
MS.gene026612	MS.gene032699	PPI
MS.gene032699	MS.gene83139	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032699.t1	MTR_6g003990	98.065	155	3	0	1	155	1	155	1.60e-113	318
MS.gene032699.t1	MTR_6g003990	98.305	118	2	0	38	155	1	118	3.32e-85	245
MS.gene032699.t1	MTR_7g083060	65.385	156	43	4	1	155	1	146	7.55e-68	202
MS.gene032699.t1	MTR_1g030630	59.355	155	55	3	1	155	1	147	1.29e-59	182
MS.gene032699.t1	MTR_5g025150	58.065	155	55	3	1	155	1	145	5.06e-59	180
MS.gene032699.t1	MTR_5g022620	51.266	158	73	2	1	155	1	157	1.10e-51	162
MS.gene032699.t1	MTR_8g091420	50.649	154	71	2	2	155	5	153	2.60e-49	155
MS.gene032699.t1	MTR_3g087770	48.701	154	73	3	2	155	5	152	2.37e-48	153
MS.gene032699.t1	MTR_4g073040	47.468	158	78	3	1	155	1	156	5.82e-46	147
MS.gene032699.t1	MTR_5g055020	47.742	155	73	3	1	155	1	147	9.40e-45	144
MS.gene032699.t1	MTR_3g117890	43.827	162	78	3	1	155	1	156	2.43e-44	143
MS.gene032699.t1	MTR_3g073670	44.156	154	77	3	2	155	5	149	7.78e-37	124
MS.gene032699.t1	MTR_1g078090	41.216	148	54	4	28	155	51	185	2.86e-27	100
MS.gene032699.t1	MTR_6g026890	39.416	137	72	4	28	155	14	148	6.16e-26	96.3
MS.gene032699.t1	MTR_2g091235	34.868	152	64	6	29	155	1	142	6.51e-21	83.2
MS.gene032699.t1	MTR_3g010130	67.797	59	11	2	1	58	1	52	1.78e-19	77.4
MS.gene032699.t1	MTR_6g045203	36.170	141	68	7	32	155	5	140	1.95e-13	63.9
MS.gene032699.t1	MTR_1g082650	41.892	74	40	2	24	95	2	74	3.63e-13	66.2
MS.gene032699.t1	MTR_1g092670	43.939	66	36	1	30	95	4	68	1.04e-12	60.5
MS.gene032699.t1	MTR_4g094238	38.043	92	55	2	29	120	1	90	2.03e-12	60.1
MS.gene032699.t1	MTR_4g117140	38.806	67	40	1	29	95	9	74	5.35e-11	60.1
MS.gene032699.t1	MTR_4g117220	38.806	67	40	1	29	95	9	74	5.91e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032699.t1	AT5G17450	64.103	156	48	4	1	155	1	149	2.28e-63	191
MS.gene032699.t1	AT1G22990	58.710	155	61	2	1	155	1	152	2.61e-61	186
MS.gene032699.t1	AT1G22990	56.129	155	60	3	1	155	1	147	2.27e-56	173
MS.gene032699.t1	AT1G71050	58.491	159	55	4	1	155	1	152	4.57e-55	170
MS.gene032699.t1	AT5G17450	67.327	101	29	3	56	155	8	105	1.04e-43	140
MS.gene032699.t1	AT5G66110	50.000	134	64	1	25	155	14	147	1.96e-43	140
MS.gene032699.t1	AT5G66110	50.000	134	64	1	25	155	14	147	1.96e-43	140
MS.gene032699.t1	AT4G39700	50.318	157	72	4	2	155	5	158	8.95e-43	139
MS.gene032699.t1	AT4G38580	43.949	157	82	2	1	155	1	153	7.19e-41	134
MS.gene032699.t1	AT4G38580	43.949	157	82	2	1	155	1	153	7.19e-41	134
MS.gene032699.t1	AT5G66110	50.820	122	55	3	38	155	1	121	9.13e-39	128
MS.gene032699.t1	AT4G35060	39.623	159	86	5	1	155	1	153	2.46e-36	123
MS.gene032699.t1	AT4G08570	38.065	155	86	4	2	155	5	150	6.53e-34	116
MS.gene032699.t1	AT4G10465	39.506	162	79	4	7	155	28	183	9.08e-28	102
MS.gene032699.t1	AT2G18196	35.897	156	67	3	20	155	36	178	2.49e-26	98.2
MS.gene032699.t1	AT1G06330	34.615	156	67	5	29	155	1	150	8.07e-23	88.6
MS.gene032699.t1	AT3G56891	35.714	154	72	6	28	155	14	166	1.13e-21	85.9
MS.gene032699.t1	AT2G18196	36.232	138	55	3	38	155	1	125	2.26e-21	84.0
MS.gene032699.t1	AT3G56891	35.417	144	66	6	38	155	1	143	2.45e-19	79.3
MS.gene032699.t1	AT1G29100	29.730	148	75	3	32	155	5	147	2.02e-13	63.9
MS.gene032699.t1	AT1G29100	28.767	146	75	3	34	155	1	141	5.57e-12	60.1
MS.gene032699.t1	AT3G02960	25.373	134	95	2	22	155	118	246	2.89e-11	60.1
MS.gene032699.t1	AT3G48970	35.507	138	75	6	31	155	4	140	4.33e-11	57.8
MS.gene032699.t1	AT3G48970	35.507	138	75	6	31	155	2	138	4.93e-11	57.4
MS.gene032699.t1	AT3G48970	35.507	138	75	6	31	155	2	138	4.93e-11	57.4
MS.gene032699.t1	AT1G66240	41.429	70	39	2	27	95	29	97	5.12e-11	56.6

Find 37 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTACATTGTCATCGTTAAAA+AGG	0.148849	6.3:-83364	None:intergenic
TTTGCGCTTGGACCTCTTAA+TGG	0.234748	6.3:-82815	None:intergenic
GGAAAGTAAGAGCTCAATTT+TGG	0.242682	6.3:+83201	MS.gene032699:CDS
AATGCAGTGACTTCATTAAA+AGG	0.253710	6.3:+83006	MS.gene032699:CDS
ATTCTCTTTAACACCTTGTT+TGG	0.261184	6.3:-83167	None:intergenic
AGAGGGTGCCCTTCTGTCAT+AGG	0.409265	6.3:-83270	None:intergenic
CGTTGTGCAGGCTGCCCCTA+TGG	0.438696	6.3:+83307	MS.gene032699:CDS
TACCTGCATTGCTTTGCGCT+TGG	0.445910	6.3:-82827	None:intergenic
TGATGATTAGATGAGGCCAT+AGG	0.474301	6.3:-83323	None:intergenic
GGACCTCTTAATGGTGGAGT+TGG	0.475031	6.3:-82806	None:intergenic
TAACCAACTCCACCATTAAG+AGG	0.490350	6.3:+82803	MS.gene032699:CDS
TCATAGGCTCCGTAGGCATA+AGG	0.499332	6.3:-83254	None:intergenic
ACTTACAGTAGTACAAAAGT+TGG	0.499673	6.3:-82779	None:intergenic
GTCCAAGCGCAAAGCAATGC+AGG	0.506452	6.3:+82825	MS.gene032699:CDS
GGTTGAGATAAAAGTGAGAA+TGG	0.506840	6.3:+82954	MS.gene032699:CDS
GATGATTAGATGAGGCCATA+GGG	0.508185	6.3:-83322	None:intergenic
TATGACAGAAGGGCACCCTC+TGG	0.508305	6.3:+83272	MS.gene032699:CDS
ACAAGTTGCTGTTCAACATA+AGG	0.511704	6.3:-83224	None:intergenic
AGGTGAACAGAAAGCAAAGC+AGG	0.515676	6.3:+83120	MS.gene032699:CDS
CAAAGCAGGGTGATAGTAAG+TGG	0.536704	6.3:+83134	MS.gene032699:CDS
CCTTCTGTCATAGGCTCCGT+AGG	0.564096	6.3:-83261	None:intergenic
ATTTGTTAGAAACGTTGTGC+AGG	0.566209	6.3:+83295	MS.gene032699:CDS
GCGCTTGGACCTCTTAATGG+TGG	0.571589	6.3:-82812	None:intergenic
GTGTACTATCCTTATGCCTA+CGG	0.578716	6.3:+83245	MS.gene032699:CDS
CAGTAGTACAAAAGTTGGAG+AGG	0.608993	6.3:-82774	None:intergenic
CTCTTCTTGATGATTAGATG+AGG	0.629560	6.3:-83330	None:intergenic
CCTACGGAGCCTATGACAGA+AGG	0.629778	6.3:+83261	MS.gene032699:CDS
ATGATTAGATGAGGCCATAG+GGG	0.651063	6.3:-83321	None:intergenic
ATGTATAGGTGTGAAATCTG+TGG	0.658222	6.3:+83097	MS.gene032699:intron
TTATCAGCTAGATTACATGA+TGG	0.658456	6.3:-83396	None:intergenic
TATAGGTGTGAAATCTGTGG+AGG	0.664803	6.3:+83100	MS.gene032699:intron
AACGTTTCTAACAAATCCAG+AGG	0.665538	6.3:-83288	None:intergenic
GGTGAACAGAAAGCAAAGCA+GGG	0.672634	6.3:+83121	MS.gene032699:CDS
CTACGGAGCCTATGACAGAA+GGG	0.679149	6.3:+83262	MS.gene032699:CDS
TGGTTATGTTGATCCAAACA+AGG	0.688407	6.3:+83154	MS.gene032699:CDS
ACGTTTCTAACAAATCCAGA+GGG	0.717248	6.3:-83287	None:intergenic
AAAGTGAGAATGGACTGTGA+TGG	0.751246	6.3:+82964	MS.gene032699:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTATATAATTTCAGA+CGG	+	chr6.3:82933-82952	MS.gene032699:intron	10.0%
!	CTATACATCAATTAATGGAA+GGG	-	chr6.3:83085-83104	None:intergenic	25.0%
!	TACATTGTCATCGTTAAAAA+GGG	-	chr6.3:83366-83385	None:intergenic	25.0%
!	TTACATTGTCATCGTTAAAA+AGG	-	chr6.3:83367-83386	None:intergenic	25.0%
!!	TTAAAGAGAATAAAAAGCAC+TGG	+	chr6.3:83179-83198	MS.gene032699:CDS	25.0%
!!!	TAAAGAGAATAAAAAGCACT+GGG	+	chr6.3:83180-83199	MS.gene032699:CDS	25.0%
	AATGCAGTGACTTCATTAAA+AGG	+	chr6.3:83006-83025	MS.gene032699:CDS	30.0%
	ACTTACAGTAGTACAAAAGT+TGG	-	chr6.3:82782-82801	None:intergenic	30.0%
	ATTCTCTTTAACACCTTGTT+TGG	-	chr6.3:83170-83189	None:intergenic	30.0%
	CACACCTATACATCAATTAA+TGG	-	chr6.3:83090-83109	None:intergenic	30.0%
	CCTATACATCAATTAATGGA+AGG	-	chr6.3:83086-83105	None:intergenic	30.0%
	CCTTCCATTAATTGATGTAT+AGG	+	chr6.3:83083-83102	MS.gene032699:intron	30.0%
	TATAGTATATCCTCCTTACT+TGG	+	chr6.3:82890-82909	MS.gene032699:intron	30.0%
	AACGTTTCTAACAAATCCAG+AGG	-	chr6.3:83291-83310	None:intergenic	35.0%
	ACGTTTCTAACAAATCCAGA+GGG	-	chr6.3:83290-83309	None:intergenic	35.0%
	ATGTATAGGTGTGAAATCTG+TGG	+	chr6.3:83097-83116	MS.gene032699:intron	35.0%
	GGTTGAGATAAAAGTGAGAA+TGG	+	chr6.3:82954-82973	MS.gene032699:CDS	35.0%
	TAGTGAGTACTAACCAAGTA+AGG	-	chr6.3:82906-82925	None:intergenic	35.0%
	TCAATTAATGGAAGGGAGTT+AGG	-	chr6.3:83078-83097	None:intergenic	35.0%
	TGGTTATGTTGATCCAAACA+AGG	+	chr6.3:83154-83173	MS.gene032699:CDS	35.0%
!	ACAAGTTGCTGTTCAACATA+AGG	-	chr6.3:83227-83246	None:intergenic	35.0%
!	ATTTGTTAGAAACGTTGTGC+AGG	+	chr6.3:83295-83314	MS.gene032699:CDS	35.0%
!	CTCTTCTTGATGATTAGATG+AGG	-	chr6.3:83333-83352	None:intergenic	35.0%
!	GGAAAGTAAGAGCTCAATTT+TGG	+	chr6.3:83201-83220	MS.gene032699:CDS	35.0%
	ATGATTAGATGAGGCCATAG+GGG	-	chr6.3:83324-83343	None:intergenic	40.0%
	CAGTAGTACAAAAGTTGGAG+AGG	-	chr6.3:82777-82796	None:intergenic	40.0%
	GATGATTAGATGAGGCCATA+GGG	-	chr6.3:83325-83344	None:intergenic	40.0%
	GTGTACTATCCTTATGCCTA+CGG	+	chr6.3:83245-83264	MS.gene032699:CDS	40.0%
	TAACCAACTCCACCATTAAG+AGG	+	chr6.3:82803-82822	MS.gene032699:CDS	40.0%
	TATAGGTGTGAAATCTGTGG+AGG	+	chr6.3:83100-83119	MS.gene032699:intron	40.0%
	TGAGTACTAACCAAGTAAGG+AGG	-	chr6.3:82903-82922	None:intergenic	40.0%
	TGATGATTAGATGAGGCCAT+AGG	-	chr6.3:83326-83345	None:intergenic	40.0%
!!	AAAGTGAGAATGGACTGTGA+TGG	+	chr6.3:82964-82983	MS.gene032699:CDS	40.0%
	AGGTGAACAGAAAGCAAAGC+AGG	+	chr6.3:83120-83139	MS.gene032699:CDS	45.0%
	CAAAGCAGGGTGATAGTAAG+TGG	+	chr6.3:83134-83153	MS.gene032699:CDS	45.0%
	GGTGAACAGAAAGCAAAGCA+GGG	+	chr6.3:83121-83140	MS.gene032699:CDS	45.0%
	TTTGCGCTTGGACCTCTTAA+TGG	-	chr6.3:82818-82837	None:intergenic	45.0%
	CTACGGAGCCTATGACAGAA+GGG	+	chr6.3:83262-83281	MS.gene032699:CDS	50.0%
	TACCTGCATTGCTTTGCGCT+TGG	-	chr6.3:82830-82849	None:intergenic	50.0%
	TCATAGGCTCCGTAGGCATA+AGG	-	chr6.3:83257-83276	None:intergenic	50.0%
!	GGACCTCTTAATGGTGGAGT+TGG	-	chr6.3:82809-82828	None:intergenic	50.0%
	CCTACGGAGCCTATGACAGA+AGG	+	chr6.3:83261-83280	MS.gene032699:CDS	55.0%
	CCTTCTGTCATAGGCTCCGT+AGG	-	chr6.3:83264-83283	None:intergenic	55.0%
	GCGCTTGGACCTCTTAATGG+TGG	-	chr6.3:82815-82834	None:intergenic	55.0%
	GTCCAAGCGCAAAGCAATGC+AGG	+	chr6.3:82825-82844	MS.gene032699:CDS	55.0%
!!	AGAGGGTGCCCTTCTGTCAT+AGG	-	chr6.3:83273-83292	None:intergenic	55.0%
!!	TATGACAGAAGGGCACCCTC+TGG	+	chr6.3:83272-83291	MS.gene032699:CDS	55.0%
	CGTTGTGCAGGCTGCCCCTA+TGG	+	chr6.3:83307-83326	MS.gene032699:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	82757	83406	82757	ID=MS.gene032699
chr6.3	mRNA	82757	83406	82757	ID=MS.gene032699.t1;Parent=MS.gene032699
chr6.3	exon	82757	82846	82757	ID=MS.gene032699.t1.exon1;Parent=MS.gene032699.t1
chr6.3	CDS	82757	82846	82757	ID=cds.MS.gene032699.t1;Parent=MS.gene032699.t1
chr6.3	exon	82952	83027	82952	ID=MS.gene032699.t1.exon2;Parent=MS.gene032699.t1
chr6.3	CDS	82952	83027	82952	ID=cds.MS.gene032699.t1;Parent=MS.gene032699.t1
chr6.3	exon	83105	83406	83105	ID=MS.gene032699.t1.exon3;Parent=MS.gene032699.t1
chr6.3	CDS	83105	83406	83105	ID=cds.MS.gene032699.t1;Parent=MS.gene032699.t1

Gene Sequence

>MS.gene032699

ATGGGTGCTCTTGATTACCTCTCCAACTTTTGTACTACTGTAAGTGTAACCAACTCCACCATTAAGAGGTCCAAGCGCAAAGCAATGCAGGTAATACTATAAGAACTTCTTTTCTTTTTTTTTGTAACTATCATATAGTATATCCTCCTTACTTGGTTAGTACTCACTAAGAGCATATTTTTTATATAATTTCAGACGGTTGAGATAAAAGTGAGAATGGACTGTGATGGTTGTGAAAGAAGAGTCAGAAATGCAGTGACTTCATTAAAAGGTTAATTAATTAGTGTGTACATAATATAGTTAGTTTACAACTAATTTCCTAACTCCCTTCCATTAATTGATGTATAGGTGTGAAATCTGTGGAGGTGAACAGAAAGCAAAGCAGGGTGATAGTAAGTGGTTATGTTGATCCAAACAAGGTGTTAAAGAGAATAAAAAGCACTGGGAAAGTAAGAGCTCAATTTTGGCCTTATGTTGAACAGCAACTTGTGTACTATCCTTATGCCTACGGAGCCTATGACAGAAGGGCACCCTCTGGATTTGTTAGAAACGTTGTGCAGGCTGCCCCTATGGCCTCATCTAATCATCAAGAAGAGATACTTGTTTCCCTTTTTAACGATGACAATGTAAATGCATGTTCCATCATGTAA

Protein sequence

>MS.gene032699.t1

MGALDYLSNFCTTVSVTNSTIKRSKRKAMQTVEIKVRMDCDGCERRVRNAVTSLKGVKSVEVNRKQSRVIVSGYVDPNKVLKRIKSTGKVRAQFWPYVEQQLVYYPYAYGAYDRRAPSGFVRNVVQAAPMASSNHQEEILVSLFNDDNVNACSIM