AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72709


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72709.t1 MTR_5g025150 99.310 145 1 0 1 145 1 145 6.46e-107 301
MS.gene72709.t1 MTR_1g030630 73.469 147 37 1 1 145 1 147 2.90e-77 226
MS.gene72709.t1 MTR_6g003990 58.710 155 54 3 1 145 1 155 1.04e-59 182
MS.gene72709.t1 MTR_7g083060 60.544 147 55 3 1 145 1 146 6.38e-58 177
MS.gene72709.t1 MTR_3g087770 51.351 148 68 1 2 145 5 152 2.00e-48 153
MS.gene72709.t1 MTR_6g003990 61.864 118 42 2 31 145 1 118 7.92e-48 150
MS.gene72709.t1 MTR_4g073040 51.282 156 65 3 1 145 1 156 6.99e-47 149
MS.gene72709.t1 MTR_8g091420 53.691 149 64 2 2 145 5 153 1.84e-46 148
MS.gene72709.t1 MTR_5g055020 46.939 147 76 1 1 145 1 147 1.18e-45 146
MS.gene72709.t1 MTR_3g117890 46.795 156 72 3 1 145 1 156 4.63e-45 144
MS.gene72709.t1 MTR_5g022620 51.572 159 61 5 1 145 1 157 1.66e-44 143
MS.gene72709.t1 MTR_3g073670 46.897 145 76 1 2 145 5 149 5.53e-39 129
MS.gene72709.t1 MTR_1g078090 40.690 145 68 4 15 145 45 185 6.82e-30 107
MS.gene72709.t1 MTR_6g026890 37.500 144 76 5 15 145 6 148 6.19e-26 95.9
MS.gene72709.t1 MTR_2g091235 33.333 147 70 3 22 145 1 142 6.28e-22 85.5
MS.gene72709.t1 MTR_3g010130 69.231 52 15 1 1 51 1 52 3.59e-18 73.9
MS.gene72709.t1 MTR_6g045203 34.559 136 74 6 25 145 5 140 1.20e-17 74.3
MS.gene72709.t1 MTR_1g082650 39.189 74 45 0 22 95 9 82 6.92e-14 67.8
MS.gene72709.t1 MTR_4g117140 40.909 66 39 0 22 87 9 74 4.30e-13 65.5
MS.gene72709.t1 MTR_2g022250 40.909 66 39 0 22 87 9 74 5.35e-13 65.5
MS.gene72709.t1 MTR_4g117220 40.909 66 39 0 22 87 9 74 6.29e-13 65.1
MS.gene72709.t1 MTR_7g108560 41.538 65 38 0 23 87 22 86 9.27e-13 64.3
MS.gene72709.t1 MTR_7g013660 38.462 91 54 1 23 113 4 92 1.46e-12 60.8
MS.gene72709.t1 MTR_1g107565 39.394 66 40 0 22 87 12 77 1.50e-12 63.9
MS.gene72709.t1 MTR_4g094238 36.471 85 51 2 22 106 1 82 5.44e-12 58.5
MS.gene72709.t1 MTR_1g092670 40.000 65 39 0 23 87 4 68 2.88e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72709.t1 AT1G22990 55.921 152 60 2 1 145 1 152 1.31e-57 176
MS.gene72709.t1 AT1G22990 53.289 152 59 3 1 145 1 147 1.07e-52 164
MS.gene72709.t1 AT1G71050 54.605 152 62 3 1 145 1 152 3.57e-52 162
MS.gene72709.t1 AT5G17450 55.034 149 63 2 1 145 1 149 2.36e-50 158
MS.gene72709.t1 AT4G39700 50.955 157 61 4 2 145 5 158 1.47e-43 141
MS.gene72709.t1 AT4G38580 47.059 153 73 3 1 145 1 153 6.01e-42 137
MS.gene72709.t1 AT4G38580 47.059 153 73 3 1 145 1 153 6.01e-42 137
MS.gene72709.t1 AT5G66110 54.412 136 54 3 17 145 13 147 1.38e-40 133
MS.gene72709.t1 AT5G66110 54.412 136 54 3 17 145 13 147 1.38e-40 133
MS.gene72709.t1 AT4G35060 41.176 153 82 3 1 145 1 153 1.13e-39 131
MS.gene72709.t1 AT4G08570 44.521 146 79 2 2 145 5 150 1.57e-38 128
MS.gene72709.t1 AT5G66110 52.459 122 50 3 31 145 1 121 4.44e-35 118
MS.gene72709.t1 AT5G17450 51.515 99 47 1 48 145 7 105 1.22e-32 111
MS.gene72709.t1 AT2G18196 38.065 155 81 4 3 145 27 178 2.02e-28 103
MS.gene72709.t1 AT4G10465 40.541 148 73 3 10 145 39 183 6.20e-28 102
MS.gene72709.t1 AT1G06330 37.662 154 62 3 22 145 1 150 2.25e-24 92.0
MS.gene72709.t1 AT2G18196 40.157 127 62 3 31 145 1 125 1.30e-23 89.4
MS.gene72709.t1 AT3G56891 33.117 154 71 5 22 145 15 166 1.60e-21 85.1
MS.gene72709.t1 AT3G56891 33.103 145 65 5 31 145 1 143 1.52e-19 79.3
MS.gene72709.t1 AT1G29100 30.405 148 71 4 25 145 5 147 1.50e-15 69.3
MS.gene72709.t1 AT3G48970 38.686 137 69 6 24 145 4 140 3.14e-14 65.9
MS.gene72709.t1 AT1G29100 29.452 146 71 4 27 145 1 141 3.24e-14 65.5
MS.gene72709.t1 AT3G48970 38.686 137 69 6 24 145 2 138 3.27e-14 65.5
MS.gene72709.t1 AT3G48970 38.686 137 69 6 24 145 2 138 3.27e-14 65.5
MS.gene72709.t1 AT1G66240 43.077 65 37 0 23 87 33 97 4.76e-12 59.3
MS.gene72709.t1 AT3G06130 36.111 72 46 0 22 93 9 80 7.44e-12 62.0
MS.gene72709.t1 AT3G06130 36.111 72 46 0 22 93 9 80 1.09e-11 61.6
MS.gene72709.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.06e-11 60.8
MS.gene72709.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.06e-11 60.8
MS.gene72709.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.06e-11 60.8
MS.gene72709.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.36e-11 60.5
MS.gene72709.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.36e-11 60.5
MS.gene72709.t1 AT3G56240 40.909 66 39 0 23 88 3 68 4.18e-11 57.0
MS.gene72709.t1 AT3G56240 40.909 66 39 0 23 88 3 68 4.18e-11 57.0

Find 31 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTATCATCTGTTCCATT+TGG 0.247141 5.4:+16305052 None:intergenic
AGTGGAAATAAAGGTGAAAA+TGG 0.297522 5.4:-16306072 MS.gene72709:CDS
CTGTGCTCTGCACCTTCCTT+AGG 0.323100 5.4:+16305200 None:intergenic
TCTTATCATCTGTTCCATTT+GGG 0.328722 5.4:+16305053 None:intergenic
CACATGATTGAACATGCATT+AGG 0.335274 5.4:+16305004 None:intergenic
CTTATAGACAGTGGAAATAA+AGG 0.347942 5.4:-16306081 MS.gene72709:intron
TCATCTGTTCCATTTGGGTT+TGG 0.349576 5.4:+16305058 None:intergenic
ACATGATTGAACATGCATTA+GGG 0.359404 5.4:+16305005 None:intergenic
CAAAGCAAGGTAACCGTGAC+TGG 0.363196 5.4:-16305244 MS.gene72709:CDS
TGGGAATGTGGATAGAAATA+AGG 0.391392 5.4:-16305224 MS.gene72709:CDS
ACCAAGTTAGCTTCAACATA+TGG 0.451325 5.4:+16305157 None:intergenic
GCCATATGTTGAAGCTAACT+TGG 0.464839 5.4:-16305158 MS.gene72709:CDS
TGCATATGCCTGTGTTATGT+AGG 0.478193 5.4:+16305126 None:intergenic
AATTCTGTTACTAACATGTC+AGG 0.503481 5.4:-16306020 MS.gene72709:intron
CTAAGGAAGGTGCAGAGCAC+AGG 0.508118 5.4:-16305199 MS.gene72709:CDS
AGGGTTATCATCACTGAAGA+AGG 0.527497 5.4:+16305024 None:intergenic
TGGATAGAAATAAGGTCCTA+AGG 0.531057 5.4:-16305216 MS.gene72709:CDS
TTAGCAATACCAAACCCAAA+TGG 0.531163 5.4:-16305067 MS.gene72709:CDS
GCATATGCCTGTGTTATGTA+GGG 0.531267 5.4:+16305127 None:intergenic
TGTAGGTGTGAAGGAAGTAG+AGG 0.545264 5.4:-16305278 MS.gene72709:intron
AAAGAAACACAAACCCATGC+AGG 0.560046 5.4:-16306186 MS.gene72709:intron
GGTAACCGTGACTGGGAATG+TGG 0.560905 5.4:-16305236 MS.gene72709:CDS
ATATGATAATGTAGGTGTGA+AGG 0.572793 5.4:-16305287 MS.gene72709:intron
TAGAAATAAGGTCCTAAGGA+AGG 0.572839 5.4:-16305212 MS.gene72709:CDS
TCTATCCACATTCCCAGTCA+CGG 0.577046 5.4:+16305231 None:intergenic
ATATGTTGAAGCTAACTTGG+TGG 0.579782 5.4:-16305155 MS.gene72709:CDS
ATGGCTGTGAAAGAAGAGTT+CGG 0.593996 5.4:-16306043 MS.gene72709:CDS
AAAGCAAGGTAACCGTGACT+GGG 0.604590 5.4:-16305243 MS.gene72709:CDS
GGTGAATAGAGAGCAAAGCA+AGG 0.638066 5.4:-16305257 MS.gene72709:CDS
GGCATATCCCTACATAACAC+AGG 0.656872 5.4:-16305134 MS.gene72709:CDS
AAGGTGAAAATGGACTGTGA+TGG 0.710877 5.4:-16306062 MS.gene72709:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTATATAAAAAAAATTCTA+AGG + chr5.4:16305711-16305730 None:intergenic 10.0%
!! TACATATACATAAATGAGAT+AGG + chr5.4:16305098-16305117 None:intergenic 20.0%
!!! AATCAAATGGCATAATTTTT+AGG - chr5.4:16305270-16305289 MS.gene72709:CDS 20.0%
!!! TTACAACAATATGATAATGT+AGG - chr5.4:16305941-16305960 MS.gene72709:intron 20.0%
!!! TTTCAAGTGAATTTTAAGTA+CGG + chr5.4:16305361-16305380 None:intergenic 20.0%
!!! TTTTCTTATCTCAAATCAAA+TGG - chr5.4:16305257-16305276 MS.gene72709:CDS 20.0%
! AATGTTCATATTCTCAATGA+AGG - chr5.4:16305309-16305328 MS.gene72709:intron 25.0%
! ATAAATGAGATAGGATACAT+AGG + chr5.4:16305089-16305108 None:intergenic 25.0%
! TAAATGAGATAGGATACATA+GGG + chr5.4:16305088-16305107 None:intergenic 25.0%
! TCATATCTTCATTGATAAGT+GGG - chr5.4:16305731-16305750 MS.gene72709:intron 25.0%
! TTGATATTTCAAACGATTCT+CGG - chr5.4:16305632-16305651 MS.gene72709:intron 25.0%
!! TGTTTTCTTTATAGCCAAAT+TGG - chr5.4:16305876-16305895 MS.gene72709:intron 25.0%
!! TTTTGTTAGAACAACCAATT+TGG + chr5.4:16305893-16305912 None:intergenic 25.0%
AAATGAGATAGGATACATAG+GGG + chr5.4:16305087-16305106 None:intergenic 30.0%
AATTCTGTTACTAACATGTC+AGG - chr5.4:16305216-16305235 MS.gene72709:CDS 30.0%
ACATGATTGAACATGCATTA+GGG + chr5.4:16306234-16306253 None:intergenic 30.0%
AGTGGAAATAAAGGTGAAAA+TGG - chr5.4:16305164-16305183 MS.gene72709:CDS 30.0%
ATCTTATCATCTGTTCCATT+TGG + chr5.4:16306187-16306206 None:intergenic 30.0%
CTCATATCTTCATTGATAAG+TGG - chr5.4:16305730-16305749 MS.gene72709:intron 30.0%
CTTATAGACAGTGGAAATAA+AGG - chr5.4:16305155-16305174 MS.gene72709:CDS 30.0%
GTTGAAACTCTATTGATCAA+CGG - chr5.4:16305381-16305400 MS.gene72709:intron 30.0%
TCTTATCATCTGTTCCATTT+GGG + chr5.4:16306186-16306205 None:intergenic 30.0%
TGATATTTCAAACGATTCTC+GGG - chr5.4:16305633-16305652 MS.gene72709:intron 30.0%
TTCATATTCTCAATGAAGGT+GGG - chr5.4:16305313-16305332 MS.gene72709:intron 30.0%
! AAATTCTAAGGGCAGATTAT+AGG + chr5.4:16305699-16305718 None:intergenic 30.0%
! AATTCTAAGGGCAGATTATA+GGG + chr5.4:16305698-16305717 None:intergenic 30.0%
! ATATGATAATGTAGGTGTGA+AGG - chr5.4:16305949-16305968 MS.gene72709:intron 30.0%
! ATGTTTTTCACATATCCAGA+AGG + chr5.4:16306145-16306164 None:intergenic 30.0%
! GGTGGGTTCTATAATTTATA+TGG - chr5.4:16305330-16305349 MS.gene72709:intron 30.0%
!!! ATGGGTTTGTGTTTCTTTTT+TGG + chr5.4:16305048-16305067 None:intergenic 30.0%
!!! CTTTTTTCTCTTATAGACAG+TGG - chr5.4:16305146-16305165 MS.gene72709:CDS 30.0%
!!! TGGGTTTGTGTTTCTTTTTT+GGG + chr5.4:16305047-16305066 None:intergenic 30.0%
ATATGTTGAAGCTAACTTGG+TGG - chr5.4:16306081-16306100 MS.gene72709:intron 35.0%
ATGGTCTCAGCCATAAAAAT+TGG - chr5.4:16305565-16305584 MS.gene72709:intron 35.0%
CACATGATTGAACATGCATT+AGG + chr5.4:16306235-16306254 None:intergenic 35.0%
GTTCATATTCTCAATGAAGG+TGG - chr5.4:16305312-16305331 MS.gene72709:intron 35.0%
TAGAAATAAGGTCCTAAGGA+AGG - chr5.4:16306024-16306043 MS.gene72709:CDS 35.0%
TATGCAAAAAATGCACCTTC+TGG - chr5.4:16306127-16306146 MS.gene72709:intron 35.0%
TGGATAGAAATAAGGTCCTA+AGG - chr5.4:16306020-16306039 MS.gene72709:intron 35.0%
TGGGAATGTGGATAGAAATA+AGG - chr5.4:16306012-16306031 MS.gene72709:intron 35.0%
TGGTCTCAGCCATAAAAATT+GGG - chr5.4:16305566-16305585 MS.gene72709:intron 35.0%
TTAGCAATACCAAACCCAAA+TGG - chr5.4:16306169-16306188 MS.gene72709:intron 35.0%
! ACCAAGTTAGCTTCAACATA+TGG + chr5.4:16306082-16306101 None:intergenic 35.0%
! CAGGAAAAAGAGCAAAGTTT+TGG - chr5.4:16306056-16306075 MS.gene72709:CDS 35.0%
!! GCTATTTCCCCCAATTTTTA+TGG + chr5.4:16305578-16305597 None:intergenic 35.0%
!! GTGTTGGTTCAACAAAAACA+AGG - chr5.4:16305468-16305487 MS.gene72709:intron 35.0%
!!! GGGTTTGTGTTTCTTTTTTG+GGG + chr5.4:16305046-16305065 None:intergenic 35.0%
AAAGAAACACAAACCCATGC+AGG - chr5.4:16305050-16305069 MS.gene72709:CDS 40.0%
AGGGTTATCATCACTGAAGA+AGG + chr5.4:16306215-16306234 None:intergenic 40.0%
ATAGGGTGAACTGCTTTGAT+AGG + chr5.4:16305681-16305700 None:intergenic 40.0%
ATGGCTGAGACCATGATAAT+TGG + chr5.4:16305559-16305578 None:intergenic 40.0%
ATGGCTGTGAAAGAAGAGTT+CGG - chr5.4:16305193-16305212 MS.gene72709:CDS 40.0%
GCATATGCCTGTGTTATGTA+GGG + chr5.4:16306112-16306131 None:intergenic 40.0%
GCCATATGTTGAAGCTAACT+TGG - chr5.4:16306078-16306097 MS.gene72709:intron 40.0%
GGTCTCAGCCATAAAAATTG+GGG - chr5.4:16305567-16305586 MS.gene72709:intron 40.0%
GTCTCAGCCATAAAAATTGG+GGG - chr5.4:16305568-16305587 MS.gene72709:intron 40.0%
TGCATATGCCTGTGTTATGT+AGG + chr5.4:16306113-16306132 None:intergenic 40.0%
! GACACTGACATGATTTGTGT+TGG - chr5.4:16305452-16305471 MS.gene72709:intron 40.0%
! TCATCTGTTCCATTTGGGTT+TGG + chr5.4:16306181-16306200 None:intergenic 40.0%
!! AAGGTGAAAATGGACTGTGA+TGG - chr5.4:16305174-16305193 MS.gene72709:CDS 40.0%
AAAGCAAGGTAACCGTGACT+GGG - chr5.4:16305993-16306012 MS.gene72709:intron 45.0%
ATTGTGAGCGTCTACTTTCG+AGG + chr5.4:16305411-16305430 None:intergenic 45.0%
CAAACGATTCTCGGGTATCT+AGG - chr5.4:16305641-16305660 MS.gene72709:intron 45.0%
CGACTCGAGACCAATTATCA+TGG - chr5.4:16305546-16305565 MS.gene72709:intron 45.0%
GGCATATCCCTACATAACAC+AGG - chr5.4:16306102-16306121 MS.gene72709:intron 45.0%
GGCTTGTGAAAAACCTGCAT+GGG + chr5.4:16305066-16305085 None:intergenic 45.0%
GGTGAATAGAGAGCAAAGCA+AGG - chr5.4:16305979-16305998 MS.gene72709:intron 45.0%
TCTATCCACATTCCCAGTCA+CGG + chr5.4:16306008-16306027 None:intergenic 45.0%
TGTAGGTGTGAAGGAAGTAG+AGG - chr5.4:16305958-16305977 MS.gene72709:intron 45.0%
!! TTATATAAAAAAAATTCTAA+GGG + chr5.4:16305710-16305729 None:intergenic 5.0%
CAAAGCAAGGTAACCGTGAC+TGG - chr5.4:16305992-16306011 MS.gene72709:intron 50.0%
GGGCTTGTGAAAAACCTGCA+TGG + chr5.4:16305067-16305086 None:intergenic 50.0%
GGGTGAACTGCTTTGATAGG+CGG + chr5.4:16305678-16305697 None:intergenic 50.0%
TCGGGTATCTAGGTTCACGA+TGG - chr5.4:16305651-16305670 MS.gene72709:intron 50.0%
CTGTGCTCTGCACCTTCCTT+AGG + chr5.4:16306039-16306058 None:intergenic 55.0%
GGTAACCGTGACTGGGAATG+TGG - chr5.4:16306000-16306019 MS.gene72709:intron 55.0%
! CTAAGGAAGGTGCAGAGCAC+AGG - chr5.4:16306037-16306056 MS.gene72709:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 16305003 16306255 16305003 ID=MS.gene72709
chr5.4 mRNA 16305003 16306255 16305003 ID=MS.gene72709.t1;Parent=MS.gene72709
chr5.4 exon 16306187 16306255 16306187 ID=MS.gene72709.t1.exon1;Parent=MS.gene72709.t1
chr5.4 CDS 16306187 16306255 16306187 ID=cds.MS.gene72709.t1;Parent=MS.gene72709.t1
chr5.4 exon 16306021 16306096 16306021 ID=MS.gene72709.t1.exon2;Parent=MS.gene72709.t1
chr5.4 CDS 16306021 16306096 16306021 ID=cds.MS.gene72709.t1;Parent=MS.gene72709.t1
chr5.4 exon 16305003 16305295 16305003 ID=MS.gene72709.t1.exon3;Parent=MS.gene72709.t1
chr5.4 CDS 16305003 16305295 16305003 ID=cds.MS.gene72709.t1;Parent=MS.gene72709.t1
Gene Sequence

>MS.gene72709

ATGGGTGCTCTAGATTTTCTTTCAGATTACTTCACTGTCACCCCAAAAAAGAAACACAAACCCATGCAGGTTTTTCACAAGCCCCTATGTATCCTATCTCATTTATGTATATGTATTAAGAAAATTCTGATTTTTTTTTGCAACTTTTTTCTCTTATAGACAGTGGAAATAAAGGTGAAAATGGACTGTGATGGCTGTGAAAGAAGAGTTCGGAATTCTGTTACTAACATGTCAGGTATATAATCATATCTCTCTTTTCTTATCTCAAATCAAATGGCATAATTTTTAGGTAGTTTACATAATAAGAATGTTCATATTCTCAATGAAGGTGGGTTCTATAATTTATATGGTGTAACCGTACTTAAAATTCACTTGAAAGTTGAAACTCTATTGATCAACGGTGCTCCTCGAAAGTAGACGCTCACAATCTTCATCGTTCTTATAGTTGAGACACTGACATGATTTGTGTTGGTTCAACAAAAACAAGGAGAAAACAAATATGAGTTGAGTTTTGAGACACATTTATTTTCTTTTGTTTTTTTTCGACTCGAGACCAATTATCATGGTCTCAGCCATAAAAATTGGGGGAAATAGCGATGTTCTACTTACAAATAGCGATGTTCTTGTCGTTGATATTTCAAACGATTCTCGGGTATCTAGGTTCACGATGGACCGCCTATCAAAGCAGTTCACCCTATAATCTGCCCTTAGAATTTTTTTTATATAACTCATATCTTCATTGATAAGTGGGCAAGCGCATGAGCGTAGCGTAGAAATTCAGAGATAAATTTGTAATCTAAATAGTAATATTTAAACACAATTTGTACTATACTCGATTATCAATTCTTATAGTTTTTGTTGTGTTTATTTATATGTTTTCTTTATAGCCAAATTGGTTGTTCTAACAAAATGATGATTAAAAGTTGAATTAAATCATTTTACAACAATATGATAATGTAGGTGTGAAGGAAGTAGAGGTGAATAGAGAGCAAAGCAAGGTAACCGTGACTGGGAATGTGGATAGAAATAAGGTCCTAAGGAAGGTGCAGAGCACAGGAAAAAGAGCAAAGTTTTGGCCATATGTTGAAGCTAACTTGGTGGCATATCCCTACATAACACAGGCATATGCAAAAAATGCACCTTCTGGATATGTGAAAAACATTGAATTAGCAATACCAAACCCAAATGGAACAGATGATAAGATCACTTCCTTCTTCAGTGATGATAACCCTAATGCATGTTCAATCATGTGA

Protein sequence

>MS.gene72709.t1

MGALDFLSDYFTVTPKKKHKPMQTVEIKVKMDCDGCERRVRNSVTNMSGVKEVEVNREQSKVTVTGNVDRNKVLRKVQSTGKRAKFWPYVEANLVAYPYITQAYAKNAPSGYVKNIELAIPNPNGTDDKITSFFSDDNPNACSIM