AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88333


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88333.t1 MTR_5g025150 99.310 145 1 0 1 145 1 145 4.35e-107 301
MS.gene88333.t1 MTR_1g030630 72.789 147 38 1 1 145 1 147 2.13e-77 226
MS.gene88333.t1 MTR_6g003990 58.065 155 55 3 1 145 1 155 1.01e-58 179
MS.gene88333.t1 MTR_7g083060 59.864 147 56 3 1 145 1 146 5.64e-57 174
MS.gene88333.t1 MTR_3g087770 50.676 148 69 1 2 145 5 152 1.42e-47 151
MS.gene88333.t1 MTR_6g003990 61.017 118 43 2 31 145 1 118 5.37e-47 148
MS.gene88333.t1 MTR_8g091420 54.362 149 63 2 2 145 5 153 1.27e-46 148
MS.gene88333.t1 MTR_4g073040 50.641 156 66 3 1 145 1 156 1.77e-46 148
MS.gene88333.t1 MTR_3g117890 47.436 156 71 3 1 145 1 156 2.62e-46 148
MS.gene88333.t1 MTR_5g055020 46.939 147 76 1 1 145 1 147 1.83e-45 145
MS.gene88333.t1 MTR_5g022620 50.314 159 63 4 1 145 1 157 8.51e-44 141
MS.gene88333.t1 MTR_3g073670 47.586 145 75 1 2 145 5 149 2.81e-39 130
MS.gene88333.t1 MTR_1g078090 40.690 145 68 4 15 145 45 185 1.52e-29 106
MS.gene88333.t1 MTR_6g026890 36.806 144 77 5 15 145 6 148 5.62e-25 93.6
MS.gene88333.t1 MTR_2g091235 32.653 147 71 3 22 145 1 142 7.15e-22 85.5
MS.gene88333.t1 MTR_3g010130 69.231 52 15 1 1 51 1 52 3.91e-18 73.9
MS.gene88333.t1 MTR_6g045203 34.058 138 72 6 25 145 5 140 4.89e-17 72.8
MS.gene88333.t1 MTR_1g082650 39.189 74 45 0 22 95 9 82 3.13e-14 68.9
MS.gene88333.t1 MTR_7g013660 39.560 91 53 1 23 113 4 92 1.91e-13 63.2
MS.gene88333.t1 MTR_4g117140 39.394 66 40 0 22 87 9 74 1.26e-12 64.3
MS.gene88333.t1 MTR_2g022250 39.394 66 40 0 22 87 9 74 1.42e-12 63.9
MS.gene88333.t1 MTR_4g117220 39.394 66 40 0 22 87 9 74 1.78e-12 63.9
MS.gene88333.t1 MTR_1g107565 39.394 66 40 0 22 87 12 77 2.37e-12 63.2
MS.gene88333.t1 MTR_7g108560 40.000 65 39 0 23 87 22 86 2.50e-12 63.2
MS.gene88333.t1 MTR_1g092670 41.538 65 38 0 23 87 4 68 4.98e-12 58.2
MS.gene88333.t1 MTR_4g094238 35.294 85 52 2 22 106 1 82 1.32e-11 57.4
MS.gene88333.t1 MTR_1g083310 35.526 76 48 1 14 88 6 81 7.44e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88333.t1 AT1G22990 55.263 152 61 2 1 145 1 152 1.25e-57 176
MS.gene88333.t1 AT1G22990 52.632 152 60 3 1 145 1 147 9.67e-53 164
MS.gene88333.t1 AT1G71050 53.947 152 63 3 1 145 1 152 2.46e-52 163
MS.gene88333.t1 AT5G17450 54.362 149 64 2 1 145 1 149 4.45e-50 157
MS.gene88333.t1 AT4G39700 50.318 157 62 4 2 145 5 158 1.47e-42 138
MS.gene88333.t1 AT4G38580 47.059 153 73 3 1 145 1 153 1.97e-41 135
MS.gene88333.t1 AT4G38580 47.059 153 73 3 1 145 1 153 1.97e-41 135
MS.gene88333.t1 AT5G66110 54.412 136 54 3 17 145 13 147 8.47e-41 134
MS.gene88333.t1 AT5G66110 54.412 136 54 3 17 145 13 147 8.47e-41 134
MS.gene88333.t1 AT4G35060 41.830 153 81 3 1 145 1 153 1.38e-40 133
MS.gene88333.t1 AT4G08570 45.205 146 78 2 2 145 5 150 9.86e-39 128
MS.gene88333.t1 AT5G66110 52.459 122 50 3 31 145 1 121 2.78e-35 119
MS.gene88333.t1 AT5G17450 50.505 99 48 1 48 145 7 105 2.68e-32 110
MS.gene88333.t1 AT2G18196 37.419 155 82 4 3 145 27 178 4.60e-28 102
MS.gene88333.t1 AT4G10465 39.865 148 74 3 10 145 39 183 2.26e-27 100
MS.gene88333.t1 AT1G06330 37.013 154 63 3 22 145 1 150 5.27e-24 91.3
MS.gene88333.t1 AT2G18196 39.370 127 63 3 31 145 1 125 3.36e-23 88.2
MS.gene88333.t1 AT3G56891 32.468 154 72 5 22 145 15 166 1.69e-21 85.1
MS.gene88333.t1 AT3G56891 32.414 145 66 5 31 145 1 143 1.77e-19 79.3
MS.gene88333.t1 AT1G29100 30.405 148 71 4 25 145 5 147 1.29e-15 69.3
MS.gene88333.t1 AT3G48970 38.686 137 69 6 24 145 2 138 2.40e-14 65.9
MS.gene88333.t1 AT3G48970 38.686 137 69 6 24 145 2 138 2.40e-14 65.9
MS.gene88333.t1 AT3G48970 39.130 138 67 7 24 145 4 140 2.43e-14 65.9
MS.gene88333.t1 AT1G29100 29.452 146 71 4 27 145 1 141 2.92e-14 65.9
MS.gene88333.t1 AT1G66240 44.615 65 36 0 23 87 33 97 7.20e-13 61.2
MS.gene88333.t1 AT3G56240 42.424 66 38 0 23 88 3 68 5.76e-12 59.3
MS.gene88333.t1 AT3G56240 42.424 66 38 0 23 88 3 68 5.76e-12 59.3
MS.gene88333.t1 AT3G06130 37.879 66 41 0 22 87 9 74 1.01e-11 61.6
MS.gene88333.t1 AT3G06130 36.111 72 46 0 22 93 9 80 1.48e-11 61.2
MS.gene88333.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.74e-11 60.5
MS.gene88333.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.74e-11 60.5
MS.gene88333.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.74e-11 60.5
MS.gene88333.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.92e-11 60.5
MS.gene88333.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.92e-11 60.5
MS.gene88333.t1 AT5G27690 32.258 62 42 0 27 88 34 95 8.06e-11 58.9

Find 32 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTATCATCTGTTCCATT+TGG 0.247141 5.3:+14736352 None:intergenic
AGTGGAAATAAAGGTGAAAA+TGG 0.297522 5.3:-14737375 MS.gene88333:CDS
CTGTGCTCTGCACCTTCCTT+AGG 0.323100 5.3:+14736500 None:intergenic
TCTTATCATCTGTTCCATTT+GGG 0.328722 5.3:+14736353 None:intergenic
CACATGATTGAACATGCATT+AGG 0.335274 5.3:+14736304 None:intergenic
CTTATAGACAGTGGAAATAA+AGG 0.347942 5.3:-14737384 MS.gene88333:intron
TCATCTGTTCCATTTGGGTT+TGG 0.349576 5.3:+14736358 None:intergenic
ACATGATTGAACATGCATTA+GGG 0.359404 5.3:+14736305 None:intergenic
CAAAGCAAGGTAACCGTGAC+TGG 0.363196 5.3:-14736544 MS.gene88333:CDS
TGGGAATGTGGATAGAAATA+AGG 0.391392 5.3:-14736524 MS.gene88333:CDS
ACCAAGTTAGCTTCAACATA+TGG 0.451325 5.3:+14736457 None:intergenic
GCCATATGTTGAAGCTAACT+TGG 0.464839 5.3:-14736458 MS.gene88333:CDS
AGCTTGTGAAATACCTGCAT+TGG 0.470539 5.3:+14737477 None:intergenic
TGCATATGCCTGTGTTATGT+AGG 0.478193 5.3:+14736426 None:intergenic
AATTCTGTTACTAACATGTC+AGG 0.503481 5.3:-14737323 MS.gene88333:intron
CTAAGGAAGGTGCAGAGCAC+AGG 0.508118 5.3:-14736499 MS.gene88333:CDS
AGGGTTATCATCACTGAAGA+AGG 0.527497 5.3:+14736324 None:intergenic
TGGATAGAAATAAGGTCCTA+AGG 0.531057 5.3:-14736516 MS.gene88333:CDS
TTAGCAATACCAAACCCAAA+TGG 0.531163 5.3:-14736367 MS.gene88333:CDS
GCATATGCCTGTGTTATGTA+GGG 0.531267 5.3:+14736427 None:intergenic
GGTAACCGTGACTGGGAATG+TGG 0.560905 5.3:-14736536 MS.gene88333:CDS
AAAGAAACACAAACCAATGC+AGG 0.567644 5.3:-14737490 MS.gene88333:intron
ATATGATAATGTAGGTGTGA+AGG 0.572793 5.3:-14736587 MS.gene88333:intron
TAGAAATAAGGTCCTAAGGA+AGG 0.572839 5.3:-14736512 MS.gene88333:CDS
TCTATCCACATTCCCAGTCA+CGG 0.577046 5.3:+14736531 None:intergenic
ATATGTTGAAGCTAACTTGG+TGG 0.579782 5.3:-14736455 MS.gene88333:CDS
ATGGCTGTGAAAGAAGAGTT+CGG 0.593996 5.3:-14737346 MS.gene88333:CDS
AAAGCAAGGTAACCGTGACT+GGG 0.604590 5.3:-14736543 MS.gene88333:CDS
TGACGGTGAAGTAATCTGAA+AGG 0.632257 5.3:+14737520 None:intergenic
TGTGAATAGAGAGCAAAGCA+AGG 0.638066 5.3:-14736557 MS.gene88333:CDS
GGCATATCCCTACATAACAC+AGG 0.656872 5.3:-14736434 MS.gene88333:CDS
AAGGTGAAAATGGACTGTGA+TGG 0.710877 5.3:-14737365 MS.gene88333:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACATATACATAAATGAGAT+AGG + chr5.3:14736398-14736417 None:intergenic 20.0%
!!! AATCAAATGGCATAATTTTT+AGG - chr5.3:14736575-14736594 MS.gene88333:CDS 20.0%
!!! AATTTCTAAGGGTAAATTAT+AGG + chr5.3:14737002-14737021 None:intergenic 20.0%
!!! ATTTCTAAGGGTAAATTATA+GGG + chr5.3:14737001-14737020 None:intergenic 20.0%
!!! TTACAACAATATGATAATGT+AGG - chr5.3:14737245-14737264 MS.gene88333:intron 20.0%
! ATAAATGAGATAGGATACAT+AGG + chr5.3:14736389-14736408 None:intergenic 25.0%
! ATTGTTCATATTCTCAATGA+AGG - chr5.3:14736615-14736634 MS.gene88333:intron 25.0%
! TCATATCTTCATTGATAAGT+GGG - chr5.3:14737035-14737054 MS.gene88333:intron 25.0%
! TCTTCTTATCTCAAATCAAA+TGG - chr5.3:14736562-14736581 MS.gene88333:CDS 25.0%
! TTGATATTTCAAACGATTCT+CGG - chr5.3:14736935-14736954 MS.gene88333:intron 25.0%
!! TGTTTTCTTTATAGCCAAAT+TGG - chr5.3:14737180-14737199 MS.gene88333:intron 25.0%
!! TTTTGTTAGAACAACCAATT+TGG + chr5.3:14737197-14737216 None:intergenic 25.0%
!!! ATTGGTTTGTGTTTCTTTTT+TGG + chr5.3:14736348-14736367 None:intergenic 25.0%
!!! CTTTCAAGTGATTTTATGTA+CGG + chr5.3:14736667-14736686 None:intergenic 25.0%
!!! TTGGTTTGTGTTTCTTTTTT+GGG + chr5.3:14736347-14736366 None:intergenic 25.0%
AATTCTGTTACTAACATGTC+AGG - chr5.3:14736517-14736536 MS.gene88333:CDS 30.0%
ACATGATTGAACATGCATTA+GGG + chr5.3:14737538-14737557 None:intergenic 30.0%
AGTGGAAATAAAGGTGAAAA+TGG - chr5.3:14736465-14736484 MS.gene88333:CDS 30.0%
ATCTTATCATCTGTTCCATT+TGG + chr5.3:14737491-14737510 None:intergenic 30.0%
CTATTTGTAAGTAGAAACTC+CGG + chr5.3:14736901-14736920 None:intergenic 30.0%
CTCATATCTTCATTGATAAG+TGG - chr5.3:14737034-14737053 MS.gene88333:intron 30.0%
CTTATAGACAGTGGAAATAA+AGG - chr5.3:14736456-14736475 MS.gene88333:CDS 30.0%
TCTTATCATCTGTTCCATTT+GGG + chr5.3:14737490-14737509 None:intergenic 30.0%
TGATATTTCAAACGATTCTC+GGG - chr5.3:14736936-14736955 MS.gene88333:intron 30.0%
TTCATATTCTCAATGAAGGT+GGG - chr5.3:14736619-14736638 MS.gene88333:intron 30.0%
! ATATGATAATGTAGGTGTGA+AGG - chr5.3:14737253-14737272 MS.gene88333:intron 30.0%
! GGTGGGTTCTATAATTTATA+CGG - chr5.3:14736636-14736655 MS.gene88333:intron 30.0%
!!! CTTTTTTCTCTTATAGACAG+TGG - chr5.3:14736447-14736466 MS.gene88333:CDS 30.0%
!!! TGGTTTGTGTTTCTTTTTTG+GGG + chr5.3:14736346-14736365 None:intergenic 30.0%
AAAGAAACACAAACCAATGC+AGG - chr5.3:14736350-14736369 MS.gene88333:CDS 35.0%
ATATGTTGAAGCTAACTTGG+TGG - chr5.3:14737385-14737404 MS.gene88333:intron 35.0%
CACATGATTGAACATGCATT+AGG + chr5.3:14737539-14737558 None:intergenic 35.0%
CGACTTGAGAACAATTATCA+CGG - chr5.3:14736851-14736870 MS.gene88333:intron 35.0%
GTGTCAGTTCAACAAAAACA+AGG - chr5.3:14736774-14736793 MS.gene88333:intron 35.0%
GTTCATATTCTCAATGAAGG+TGG - chr5.3:14736618-14736637 MS.gene88333:intron 35.0%
TAGAAATAAGGTCCTAAGGA+AGG - chr5.3:14737328-14737347 MS.gene88333:CDS 35.0%
TATGCAAAAAATGCACCTTC+TGG - chr5.3:14737431-14737450 MS.gene88333:intron 35.0%
TGGATAGAAATAAGGTCCTA+AGG - chr5.3:14737324-14737343 MS.gene88333:CDS 35.0%
TGGGAATGTGGATAGAAATA+AGG - chr5.3:14737316-14737335 MS.gene88333:intron 35.0%
TTAGAAACTCTATCGACCAA+TGG - chr5.3:14736687-14736706 MS.gene88333:intron 35.0%
TTAGCAATACCAAACCCAAA+TGG - chr5.3:14737473-14737492 MS.gene88333:intron 35.0%
! ACCAAGTTAGCTTCAACATA+TGG + chr5.3:14737386-14737405 None:intergenic 35.0%
! CAGGAAAAAGAGCAAAGTTT+TGG - chr5.3:14737360-14737379 MS.gene88333:CDS 35.0%
! GTGTTTTTCACATATCCAGA+AGG + chr5.3:14737449-14737468 None:intergenic 35.0%
ACGGTCTCAGCGATAAAAAT+CGG - chr5.3:14736870-14736889 MS.gene88333:intron 40.0%
AGCTTGTGAAATACCTGCAT+TGG + chr5.3:14736366-14736385 None:intergenic 40.0%
AGGGTTATCATCACTGAAGA+AGG + chr5.3:14737519-14737538 None:intergenic 40.0%
ATAGGGTGAACTGCTTTGAT+AGG + chr5.3:14736984-14737003 None:intergenic 40.0%
ATGGCTGTGAAAGAAGAGTT+CGG - chr5.3:14736494-14736513 MS.gene88333:CDS 40.0%
ATTGTGAACGTCTACTTTCG+AGG + chr5.3:14736717-14736736 None:intergenic 40.0%
GCATATGCCTGTGTTATGTA+GGG + chr5.3:14737416-14737435 None:intergenic 40.0%
GCCATATGTTGAAGCTAACT+TGG - chr5.3:14737382-14737401 MS.gene88333:intron 40.0%
TGACGGTGAAGTAATCTGAA+AGG + chr5.3:14736323-14736342 None:intergenic 40.0%
TGCATATGCCTGTGTTATGT+AGG + chr5.3:14737417-14737436 None:intergenic 40.0%
TGTGAATAGAGAGCAAAGCA+AGG - chr5.3:14737283-14737302 MS.gene88333:intron 40.0%
! TCATCTGTTCCATTTGGGTT+TGG + chr5.3:14737485-14737504 None:intergenic 40.0%
!! AAGGTGAAAATGGACTGTGA+TGG - chr5.3:14736475-14736494 MS.gene88333:CDS 40.0%
!!! GTGTTTCTTTTTTGGGGTGA+CGG + chr5.3:14736340-14736359 None:intergenic 40.0%
AAAGCAAGGTAACCGTGACT+GGG - chr5.3:14737297-14737316 MS.gene88333:intron 45.0%
CAAACGATTCTCGGGTATCT+AGG - chr5.3:14736944-14736963 MS.gene88333:intron 45.0%
CGGTCTCAGCGATAAAAATC+GGG - chr5.3:14736871-14736890 MS.gene88333:intron 45.0%
GGCATATCCCTACATAACAC+AGG - chr5.3:14737406-14737425 MS.gene88333:intron 45.0%
TCGGGTATCTAGGTTCATGA+TGG - chr5.3:14736954-14736973 MS.gene88333:intron 45.0%
TCTATCCACATTCCCAGTCA+CGG + chr5.3:14737312-14737331 None:intergenic 45.0%
!! GCGATAAAAATCGGGTTAGC+CGG - chr5.3:14736879-14736898 MS.gene88333:intron 45.0%
!! ATATATAAAAAAAATTTCTA+AGG + chr5.3:14737014-14737033 None:intergenic 5.0%
!! TATATAAAAAAAATTTCTAA+GGG + chr5.3:14737013-14737032 None:intergenic 5.0%
CAAAGCAAGGTAACCGTGAC+TGG - chr5.3:14737296-14737315 MS.gene88333:intron 50.0%
GGGTGAACTGCTTTGATAGG+CGG + chr5.3:14736981-14737000 None:intergenic 50.0%
!! CTACTTTCGAGGAGCACCAT+TGG + chr5.3:14736706-14736725 None:intergenic 50.0%
CTGTGCTCTGCACCTTCCTT+AGG + chr5.3:14737343-14737362 None:intergenic 55.0%
GGTAACCGTGACTGGGAATG+TGG - chr5.3:14737304-14737323 MS.gene88333:intron 55.0%
! CTAAGGAAGGTGCAGAGCAC+AGG - chr5.3:14737341-14737360 MS.gene88333:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 14736303 14737559 14736303 ID=MS.gene88333
chr5.3 mRNA 14736303 14737559 14736303 ID=MS.gene88333.t1;Parent=MS.gene88333
chr5.3 exon 14737491 14737559 14737491 ID=MS.gene88333.t1.exon1;Parent=MS.gene88333.t1
chr5.3 CDS 14737491 14737559 14737491 ID=cds.MS.gene88333.t1;Parent=MS.gene88333.t1
chr5.3 exon 14737324 14737399 14737324 ID=MS.gene88333.t1.exon2;Parent=MS.gene88333.t1
chr5.3 CDS 14737324 14737399 14737324 ID=cds.MS.gene88333.t1;Parent=MS.gene88333.t1
chr5.3 exon 14736303 14736595 14736303 ID=MS.gene88333.t1.exon3;Parent=MS.gene88333.t1
chr5.3 CDS 14736303 14736595 14736303 ID=cds.MS.gene88333.t1;Parent=MS.gene88333.t1
Gene Sequence

>MS.gene88333

ATGGGTGCTCTAGATTTCCTTTCAGATTACTTCACCGTCACCCCAAAAAAGAAACACAAACCAATGCAGGTATTTCACAAGCTCCTATGTATCCTATCTCATTTATGTATATGTATTAAGAAAATTCTGATTTTTTTTTTGCAACTTTTTTCTCTTATAGACAGTGGAAATAAAGGTGAAAATGGACTGTGATGGCTGTGAAAGAAGAGTTCGGAATTCTGTTACTAACATGTCAGGTATATAATCATATCTCTCTCTCTCTTCTTATCTCAAATCAAATGGCATAATTTTTAGGTAGTTTACATAATAAGAATTGTTCATATTCTCAATGAAGGTGGGTTCTATAATTTATACGGTGTAACCGTACATAAAATCACTTGAAAGTTAGAAACTCTATCGACCAATGGTGCTCCTCGAAAGTAGACGTTCACAATCTTCATCGTTCTTATAGTTGAGACACTGACATGATCTGTGTCAGTTCAACAAAAACAAGGAGAAAACAAATATGACTCGAGTTTTGAGACACGTTTATTTTCTTTTGTTTTTTTCGACTTGAGAACAATTATCACGGTCTCAGCGATAAAAATCGGGTTAGCCGGAGTTTCTACTTACAAATAGCGATGTTCTTGTCGTTGATATTTCAAACGATTCTCGGGTATCTAGGTTCATGATGGACCGCCTATCAAAGCAGTTCACCCTATAATTTACCCTTAGAAATTTTTTTTATATATCTCATATCTTCATTGATAAGTGGGCAAGCGCTTGAGCGTAGCGTAGAAATTCAGAGATAAATTTGTAATCTAAATAGTAATATTTAAACACAATTTGTACTATACTCGATTATCAATTCTTATAGTTTTTGTTGTGTTTATTTATATGTTTTCTTTATAGCCAAATTGGTTGTTCTAACAAAATGATGATTAATAGTTGAATTAAATCATTTTACAACAATATGATAATGTAGGTGTGAAGGAAGTAGATGTGAATAGAGAGCAAAGCAAGGTAACCGTGACTGGGAATGTGGATAGAAATAAGGTCCTAAGGAAGGTGCAGAGCACAGGAAAAAGAGCAAAGTTTTGGCCATATGTTGAAGCTAACTTGGTGGCATATCCCTACATAACACAGGCATATGCAAAAAATGCACCTTCTGGATATGTGAAAAACACTGAATTAGCAATACCAAACCCAAATGGAACAGATGATAAGATCACTTCCTTCTTCAGTGATGATAACCCTAATGCATGTTCAATCATGTGA

Protein sequence

>MS.gene88333.t1

MGALDFLSDYFTVTPKKKHKPMQTVEIKVKMDCDGCERRVRNSVTNMSGVKEVDVNREQSKVTVTGNVDRNKVLRKVQSTGKRAKFWPYVEANLVAYPYITQAYAKNAPSGYVKNTELAIPNPNGTDDKITSFFSDDNPNACSIM