AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86494


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86494.t1 MTR_5g025150 99.310 145 1 0 1 145 1 145 4.35e-107 301
MS.gene86494.t1 MTR_1g030630 72.789 147 38 1 1 145 1 147 2.13e-77 226
MS.gene86494.t1 MTR_6g003990 58.065 155 55 3 1 145 1 155 1.01e-58 179
MS.gene86494.t1 MTR_7g083060 59.864 147 56 3 1 145 1 146 5.64e-57 174
MS.gene86494.t1 MTR_3g087770 50.676 148 69 1 2 145 5 152 1.42e-47 151
MS.gene86494.t1 MTR_6g003990 61.017 118 43 2 31 145 1 118 5.37e-47 148
MS.gene86494.t1 MTR_8g091420 54.362 149 63 2 2 145 5 153 1.27e-46 148
MS.gene86494.t1 MTR_4g073040 50.641 156 66 3 1 145 1 156 1.77e-46 148
MS.gene86494.t1 MTR_3g117890 47.436 156 71 3 1 145 1 156 2.62e-46 148
MS.gene86494.t1 MTR_5g055020 46.939 147 76 1 1 145 1 147 1.83e-45 145
MS.gene86494.t1 MTR_5g022620 50.314 159 63 4 1 145 1 157 8.51e-44 141
MS.gene86494.t1 MTR_3g073670 47.586 145 75 1 2 145 5 149 2.81e-39 130
MS.gene86494.t1 MTR_1g078090 40.690 145 68 4 15 145 45 185 1.52e-29 106
MS.gene86494.t1 MTR_6g026890 36.806 144 77 5 15 145 6 148 5.62e-25 93.6
MS.gene86494.t1 MTR_2g091235 32.653 147 71 3 22 145 1 142 7.15e-22 85.5
MS.gene86494.t1 MTR_3g010130 69.231 52 15 1 1 51 1 52 3.91e-18 73.9
MS.gene86494.t1 MTR_6g045203 34.058 138 72 6 25 145 5 140 4.89e-17 72.8
MS.gene86494.t1 MTR_1g082650 39.189 74 45 0 22 95 9 82 3.13e-14 68.9
MS.gene86494.t1 MTR_7g013660 39.560 91 53 1 23 113 4 92 1.91e-13 63.2
MS.gene86494.t1 MTR_4g117140 39.394 66 40 0 22 87 9 74 1.26e-12 64.3
MS.gene86494.t1 MTR_2g022250 39.394 66 40 0 22 87 9 74 1.42e-12 63.9
MS.gene86494.t1 MTR_4g117220 39.394 66 40 0 22 87 9 74 1.78e-12 63.9
MS.gene86494.t1 MTR_1g107565 39.394 66 40 0 22 87 12 77 2.37e-12 63.2
MS.gene86494.t1 MTR_7g108560 40.000 65 39 0 23 87 22 86 2.50e-12 63.2
MS.gene86494.t1 MTR_1g092670 41.538 65 38 0 23 87 4 68 4.98e-12 58.2
MS.gene86494.t1 MTR_4g094238 35.294 85 52 2 22 106 1 82 1.32e-11 57.4
MS.gene86494.t1 MTR_1g083310 35.526 76 48 1 14 88 6 81 7.44e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86494.t1 AT1G22990 55.263 152 61 2 1 145 1 152 1.25e-57 176
MS.gene86494.t1 AT1G22990 52.632 152 60 3 1 145 1 147 9.67e-53 164
MS.gene86494.t1 AT1G71050 53.947 152 63 3 1 145 1 152 2.46e-52 163
MS.gene86494.t1 AT5G17450 54.362 149 64 2 1 145 1 149 4.45e-50 157
MS.gene86494.t1 AT4G39700 50.318 157 62 4 2 145 5 158 1.47e-42 138
MS.gene86494.t1 AT4G38580 47.059 153 73 3 1 145 1 153 1.97e-41 135
MS.gene86494.t1 AT4G38580 47.059 153 73 3 1 145 1 153 1.97e-41 135
MS.gene86494.t1 AT5G66110 54.412 136 54 3 17 145 13 147 8.47e-41 134
MS.gene86494.t1 AT5G66110 54.412 136 54 3 17 145 13 147 8.47e-41 134
MS.gene86494.t1 AT4G35060 41.830 153 81 3 1 145 1 153 1.38e-40 133
MS.gene86494.t1 AT4G08570 45.205 146 78 2 2 145 5 150 9.86e-39 128
MS.gene86494.t1 AT5G66110 52.459 122 50 3 31 145 1 121 2.78e-35 119
MS.gene86494.t1 AT5G17450 50.505 99 48 1 48 145 7 105 2.68e-32 110
MS.gene86494.t1 AT2G18196 37.419 155 82 4 3 145 27 178 4.60e-28 102
MS.gene86494.t1 AT4G10465 39.865 148 74 3 10 145 39 183 2.26e-27 100
MS.gene86494.t1 AT1G06330 37.013 154 63 3 22 145 1 150 5.27e-24 91.3
MS.gene86494.t1 AT2G18196 39.370 127 63 3 31 145 1 125 3.36e-23 88.2
MS.gene86494.t1 AT3G56891 32.468 154 72 5 22 145 15 166 1.69e-21 85.1
MS.gene86494.t1 AT3G56891 32.414 145 66 5 31 145 1 143 1.77e-19 79.3
MS.gene86494.t1 AT1G29100 30.405 148 71 4 25 145 5 147 1.29e-15 69.3
MS.gene86494.t1 AT3G48970 38.686 137 69 6 24 145 2 138 2.40e-14 65.9
MS.gene86494.t1 AT3G48970 38.686 137 69 6 24 145 2 138 2.40e-14 65.9
MS.gene86494.t1 AT3G48970 39.130 138 67 7 24 145 4 140 2.43e-14 65.9
MS.gene86494.t1 AT1G29100 29.452 146 71 4 27 145 1 141 2.92e-14 65.9
MS.gene86494.t1 AT1G66240 44.615 65 36 0 23 87 33 97 7.20e-13 61.2
MS.gene86494.t1 AT3G56240 42.424 66 38 0 23 88 3 68 5.76e-12 59.3
MS.gene86494.t1 AT3G56240 42.424 66 38 0 23 88 3 68 5.76e-12 59.3
MS.gene86494.t1 AT3G06130 37.879 66 41 0 22 87 9 74 1.01e-11 61.6
MS.gene86494.t1 AT3G06130 36.111 72 46 0 22 93 9 80 1.48e-11 61.2
MS.gene86494.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.74e-11 60.5
MS.gene86494.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.74e-11 60.5
MS.gene86494.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.74e-11 60.5
MS.gene86494.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.92e-11 60.5
MS.gene86494.t1 AT5G19090 37.879 66 41 0 22 87 9 74 2.92e-11 60.5
MS.gene86494.t1 AT5G27690 32.258 62 42 0 27 88 34 95 8.06e-11 58.9

Find 31 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTATCATCTGTTCCATT+TGG 0.247141 5.1:+15618111 None:intergenic
AGTGGAAATAAAGGTGAAAA+TGG 0.297522 5.1:-15619123 MS.gene86494:CDS
CTGTGCTCTGCACCTTCCTT+AGG 0.323100 5.1:+15618259 None:intergenic
TCTTATCATCTGTTCCATTT+GGG 0.328722 5.1:+15618112 None:intergenic
CACATGATTGAACATGCATT+AGG 0.335274 5.1:+15618063 None:intergenic
CTTATAGACAGTGGAAATAA+AGG 0.347942 5.1:-15619132 MS.gene86494:intron
TCATCTGTTCCATTTGGGTT+TGG 0.349576 5.1:+15618117 None:intergenic
ACATGATTGAACATGCATTA+GGG 0.359404 5.1:+15618064 None:intergenic
CAAAGCAAGGTAACCGTGAC+TGG 0.363196 5.1:-15618303 MS.gene86494:CDS
TGGGAATGTCGATAGAAATA+AGG 0.392805 5.1:-15618283 MS.gene86494:CDS
ACCAAGTTAGCTTCAACATA+TGG 0.451325 5.1:+15618216 None:intergenic
GCCATATGTTGAAGCTAACT+TGG 0.464839 5.1:-15618217 MS.gene86494:CDS
AGCTTGTGAAATACCTGCAT+TGG 0.470539 5.1:+15619225 None:intergenic
TGCATATGCCTGTGTTATGT+AGG 0.478193 5.1:+15618185 None:intergenic
AATTCTGTTACTAACATGTC+AGG 0.503481 5.1:-15619071 MS.gene86494:intron
CTAAGGAAGGTGCAGAGCAC+AGG 0.508118 5.1:-15618258 MS.gene86494:CDS
AGGGTTATCATCACTGAAGA+AGG 0.527497 5.1:+15618083 None:intergenic
TTAGCAATACCAAACCCAAA+TGG 0.531163 5.1:-15618126 MS.gene86494:CDS
GCATATGCCTGTGTTATGTA+GGG 0.531267 5.1:+15618186 None:intergenic
TCGATAGAAATAAGGTCCTA+AGG 0.542836 5.1:-15618275 MS.gene86494:CDS
AAAGAAACACAAACCAATGC+AGG 0.567644 5.1:-15619238 MS.gene86494:intron
ATATGATAATGTAGGTGTGA+AGG 0.572793 5.1:-15618346 MS.gene86494:intron
TAGAAATAAGGTCCTAAGGA+AGG 0.572839 5.1:-15618271 MS.gene86494:CDS
ATATGTTGAAGCTAACTTGG+TGG 0.579782 5.1:-15618214 MS.gene86494:CDS
TCTATCGACATTCCCAGTCA+CGG 0.589743 5.1:+15618290 None:intergenic
ATGGCTGTGAAAGAAGAGTT+CGG 0.593996 5.1:-15619094 MS.gene86494:CDS
AAAGCAAGGTAACCGTGACT+GGG 0.604590 5.1:-15618302 MS.gene86494:CDS
TGACAGTGAAGTAATCTGAA+AGG 0.632257 5.1:+15619268 None:intergenic
TGTGAATAGAGAGCAAAGCA+AGG 0.638066 5.1:-15618316 MS.gene86494:CDS
GGCATATCCCTACATAACAC+AGG 0.656872 5.1:-15618193 MS.gene86494:CDS
AAGGTGAAAATGGACTGTGA+TGG 0.710877 5.1:-15619113 MS.gene86494:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACAACAATATGATAATGT+AGG - chr5.1:15618993-15619012 MS.gene86494:intron 20.0%
! AATGTTCATATTCTCAATGA+AGG - chr5.1:15618383-15618402 MS.gene86494:intron 25.0%
! ATAAATGAGATAGGATACAT+AGG + chr5.1:15618148-15618167 None:intergenic 25.0%
! TACATGTACATAAATGAGAT+AGG + chr5.1:15618157-15618176 None:intergenic 25.0%
! TCTTCTTATCTCAAATCAAA+TGG - chr5.1:15618331-15618350 MS.gene86494:CDS 25.0%
! TTGATATTTCAAACGATTCT+CGG - chr5.1:15618683-15618702 MS.gene86494:intron 25.0%
!! AATCAAATGGCATGATTTTT+AGG - chr5.1:15618344-15618363 MS.gene86494:intron 25.0%
!! AATTTCTAAGGGCAAATTAT+AGG + chr5.1:15618750-15618769 None:intergenic 25.0%
!! ATTTCTAAGGGCAAATTATA+GGG + chr5.1:15618749-15618768 None:intergenic 25.0%
!! TGTTTTCTTTATAGCCAAAT+TGG - chr5.1:15618928-15618947 MS.gene86494:intron 25.0%
!! TTTTGTTAGAACAACCAATT+TGG + chr5.1:15618945-15618964 None:intergenic 25.0%
!!! ATTGGTTTGTGTTTCTTTTT+TGG + chr5.1:15618107-15618126 None:intergenic 25.0%
!!! TTGGTTTGTGTTTCTTTTTT+GGG + chr5.1:15618106-15618125 None:intergenic 25.0%
!!! TTTCAAGTGGTTTTTAAGTA+CGG + chr5.1:15618435-15618454 None:intergenic 25.0%
AATTCTGTTACTAACATGTC+AGG - chr5.1:15618276-15618295 MS.gene86494:CDS 30.0%
ACATGATTGAACATGCATTA+GGG + chr5.1:15619286-15619305 None:intergenic 30.0%
AGTGGAAATAAAGGTGAAAA+TGG - chr5.1:15618224-15618243 MS.gene86494:CDS 30.0%
ATAGAGTTTCAACTTTCAAG+TGG + chr5.1:15618448-15618467 None:intergenic 30.0%
ATCTTATCATCTGTTCCATT+TGG + chr5.1:15619239-15619258 None:intergenic 30.0%
CTATTTGTAAGTAGAAACTC+CGG + chr5.1:15618649-15618668 None:intergenic 30.0%
CTTATAGACAGTGGAAATAA+AGG - chr5.1:15618215-15618234 MS.gene86494:CDS 30.0%
GTGTTAGTTCAACAAAAACA+AGG - chr5.1:15618522-15618541 MS.gene86494:intron 30.0%
TCTTATCATCTGTTCCATTT+GGG + chr5.1:15619238-15619257 None:intergenic 30.0%
TGATATTTCAAACGATTCTC+GGG - chr5.1:15618684-15618703 MS.gene86494:intron 30.0%
TTCATATTCTCAATGAAGGT+GGG - chr5.1:15618387-15618406 MS.gene86494:intron 30.0%
! ATATGATAATGTAGGTGTGA+AGG - chr5.1:15619001-15619020 MS.gene86494:intron 30.0%
! GGTGGGTTCTATAATTTATA+CGG - chr5.1:15618404-15618423 MS.gene86494:intron 30.0%
! TTAACCAGATTTTTATGGCT+GGG + chr5.1:15618626-15618645 None:intergenic 30.0%
!! CGAATATGAGTTGAGTTTTA+AGG - chr5.1:15618551-15618570 MS.gene86494:intron 30.0%
!!! CTTTTTTCTCTTATAGACAG+TGG - chr5.1:15618206-15618225 MS.gene86494:CDS 30.0%
!!! TGGTTTGTGTTTCTTTTTTG+GGG + chr5.1:15618105-15618124 None:intergenic 30.0%
AAAGAAACACAAACCAATGC+AGG - chr5.1:15618109-15618128 MS.gene86494:CDS 35.0%
ATATGTTGAAGCTAACTTGG+TGG - chr5.1:15619133-15619152 MS.gene86494:intron 35.0%
CACATGATTGAACATGCATT+AGG + chr5.1:15619287-15619306 None:intergenic 35.0%
CTCCTATCTTCATTGATAAG+TGG - chr5.1:15618782-15618801 MS.gene86494:intron 35.0%
GCCATAAAAATCTGGTTAAC+CGG - chr5.1:15618627-15618646 MS.gene86494:intron 35.0%
GTCCACTTATCAATGAAGAT+AGG + chr5.1:15618787-15618806 None:intergenic 35.0%
GTTCATATTCTCAATGAAGG+TGG - chr5.1:15618386-15618405 MS.gene86494:intron 35.0%
TAGAAATAAGGTCCTAAGGA+AGG - chr5.1:15619076-15619095 MS.gene86494:CDS 35.0%
TATGCAAAAAATGCACCTTC+TGG - chr5.1:15619179-15619198 MS.gene86494:intron 35.0%
TCGATAGAAATAAGGTCCTA+AGG - chr5.1:15619072-15619091 MS.gene86494:CDS 35.0%
TGACAGTGAAGTAATCTGAA+AGG + chr5.1:15618082-15618101 None:intergenic 35.0%
TGGGAATGTCGATAGAAATA+AGG - chr5.1:15619064-15619083 MS.gene86494:intron 35.0%
TTAGCAATACCAAACCCAAA+TGG - chr5.1:15619221-15619240 MS.gene86494:intron 35.0%
! ACCAAGTTAGCTTCAACATA+TGG + chr5.1:15619134-15619153 None:intergenic 35.0%
! CAGGAAAAAGAGCAAAGTTT+TGG - chr5.1:15619108-15619127 MS.gene86494:CDS 35.0%
! GTGTTTTTCACATATCCAGA+AGG + chr5.1:15619197-15619216 None:intergenic 35.0%
! GTTAACCAGATTTTTATGGC+TGG + chr5.1:15618627-15618646 None:intergenic 35.0%
! TCCGGTTAACCAGATTTTTA+TGG + chr5.1:15618631-15618650 None:intergenic 35.0%
ACGTTGAAGATTGTGAACGT+TGG + chr5.1:15618474-15618493 None:intergenic 40.0%
AGCTTGTGAAATACCTGCAT+TGG + chr5.1:15618125-15618144 None:intergenic 40.0%
AGGGTTATCATCACTGAAGA+AGG + chr5.1:15619267-15619286 None:intergenic 40.0%
ATAGGGTGAACTGCTTTGAT+AGG + chr5.1:15618732-15618751 None:intergenic 40.0%
ATGGCTGTGAAAGAAGAGTT+CGG - chr5.1:15618253-15618272 MS.gene86494:CDS 40.0%
CGACTCAAGACCAATTATCA+CGG - chr5.1:15618599-15618618 MS.gene86494:intron 40.0%
GCATATGCCTGTGTTATGTA+GGG + chr5.1:15619164-15619183 None:intergenic 40.0%
GCCATATGTTGAAGCTAACT+TGG - chr5.1:15619130-15619149 MS.gene86494:intron 40.0%
TGCATATGCCTGTGTTATGT+AGG + chr5.1:15619165-15619184 None:intergenic 40.0%
TGTGAATAGAGAGCAAAGCA+AGG - chr5.1:15619031-15619050 MS.gene86494:intron 40.0%
! TCATCTGTTCCATTTGGGTT+TGG + chr5.1:15619233-15619252 None:intergenic 40.0%
!! AAGGTGAAAATGGACTGTGA+TGG - chr5.1:15618234-15618253 MS.gene86494:CDS 40.0%
AAAGCAAGGTAACCGTGACT+GGG - chr5.1:15619045-15619064 MS.gene86494:intron 45.0%
CAAACGATTCTCGGGTATCT+AGG - chr5.1:15618692-15618711 MS.gene86494:intron 45.0%
GGCATATCCCTACATAACAC+AGG - chr5.1:15619154-15619173 MS.gene86494:intron 45.0%
TCGGGTATCTAGGTTCATGA+TGG - chr5.1:15618702-15618721 MS.gene86494:intron 45.0%
TCTATCGACATTCCCAGTCA+CGG + chr5.1:15619060-15619079 None:intergenic 45.0%
!! TATATAAAAAAAATTTCTAA+GGG + chr5.1:15618761-15618780 None:intergenic 5.0%
!! TTATATAAAAAAAATTTCTA+AGG + chr5.1:15618762-15618781 None:intergenic 5.0%
ATGGCTGGGACCGTGATAAT+TGG + chr5.1:15618612-15618631 None:intergenic 50.0%
CAAAGCAAGGTAACCGTGAC+TGG - chr5.1:15619044-15619063 MS.gene86494:intron 50.0%
CGGTCCCAGCCATAAAAATC+TGG - chr5.1:15618619-15618638 MS.gene86494:intron 50.0%
GGGTGAACTGCTTTGATAGG+CGG + chr5.1:15618729-15618748 None:intergenic 50.0%
CTGTGCTCTGCACCTTCCTT+AGG + chr5.1:15619091-15619110 None:intergenic 55.0%
! CTAAGGAAGGTGCAGAGCAC+AGG - chr5.1:15619089-15619108 MS.gene86494:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 15618062 15619307 15618062 ID=MS.gene86494
chr5.1 mRNA 15618062 15619307 15618062 ID=MS.gene86494.t1;Parent=MS.gene86494
chr5.1 exon 15619239 15619307 15619239 ID=MS.gene86494.t1.exon1;Parent=MS.gene86494.t1
chr5.1 CDS 15619239 15619307 15619239 ID=cds.MS.gene86494.t1;Parent=MS.gene86494.t1
chr5.1 exon 15619072 15619147 15619072 ID=MS.gene86494.t1.exon2;Parent=MS.gene86494.t1
chr5.1 CDS 15619072 15619147 15619072 ID=cds.MS.gene86494.t1;Parent=MS.gene86494.t1
chr5.1 exon 15618062 15618354 15618062 ID=MS.gene86494.t1.exon3;Parent=MS.gene86494.t1
chr5.1 CDS 15618062 15618354 15618062 ID=cds.MS.gene86494.t1;Parent=MS.gene86494.t1
Gene Sequence

>MS.gene86494

ATGGGTGCTCTAGATTTCCTTTCAGATTACTTCACTGTCACCCCAAAAAAGAAACACAAACCAATGCAGGTATTTCACAAGCTCCTATGTATCCTATCTCATTTATGTACATGTATTAAGAAAATTCTGATTTTTTTTTTGCAACTTTTTTCTCTTATAGACAGTGGAAATAAAGGTGAAAATGGACTGTGATGGCTGTGAAAGAAGAGTTCGGAATTCTGTTACTAACATGTCAGGTATATAATCATATCTCTCTCTCTCTCTCTCTCTCTTCTTATCTCAAATCAAATGGCATGATTTTTAGGTAGTTTACATAATAAGAATGTTCATATTCTCAATGAAGGTGGGTTCTATAATTTATACGGTGTAACCGTACTTAAAAACCACTTGAAAGTTGAAACTCTATCAACCAACGTTCACAATCTTCAACGTTCTTATAGTTGAGATAGTGACATGATTTGTGTTAGTTCAACAAAAACAAGGAGAAAACGAATATGAGTTGAGTTTTAAGGCACATTTATTTTCTTTTGTTTTTTTCGACTCAAGACCAATTATCACGGTCCCAGCCATAAAAATCTGGTTAACCGGAGTTTCTACTTACAAATAGCGATGTTCTTGTCGTTGATATTTCAAACGATTCTCGGGTATCTAGGTTCATGATGGACCGCCTATCAAAGCAGTTCACCCTATAATTTGCCCTTAGAAATTTTTTTTATATAACTCCTATCTTCATTGATAAGTGGACAAGCGCTTGAGCGTAGCGTAGAAATTCAGAGATAAATTTGTAATCTAAATAGTAATATTTAAACACAATTTGTACTATACTCGATTATCAATTCTTATAGTTTTTGTTGTGTTTATTTATATGTTTTCTTTATAGCCAAATTGGTTGTTCTAACAAAATGATGATTAATAGTTGAATTAAATCATTTTACAACAATATGATAATGTAGGTGTGAAGGAAGTAGATGTGAATAGAGAGCAAAGCAAGGTAACCGTGACTGGGAATGTCGATAGAAATAAGGTCCTAAGGAAGGTGCAGAGCACAGGAAAAAGAGCAAAGTTTTGGCCATATGTTGAAGCTAACTTGGTGGCATATCCCTACATAACACAGGCATATGCAAAAAATGCACCTTCTGGATATGTGAAAAACACTGAATTAGCAATACCAAACCCAAATGGAACAGATGATAAGATCACTTCCTTCTTCAGTGATGATAACCCTAATGCATGTTCAATCATGTGA

Protein sequence

>MS.gene86494.t1

MGALDFLSDYFTVTPKKKHKPMQTVEIKVKMDCDGCERRVRNSVTNMSGVKEVDVNREQSKVTVTGNVDRNKVLRKVQSTGKRAKFWPYVEANLVAYPYITQAYAKNAPSGYVKNTELAIPNPNGTDDKITSFFSDDNPNACSIM