AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83138

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83138.t1 MTR_1g030630 96.599 147 5 0 1 147 1 147 6.39e-106 298
MS.gene83138.t1 MTR_5g025150 72.109 147 39 1 1 147 1 145 7.15e-77 225
MS.gene83138.t1 MTR_7g083060 62.162 148 53 3 1 147 1 146 1.15e-58 179
MS.gene83138.t1 MTR_6g003990 59.355 155 55 4 1 147 1 155 7.30e-58 177
MS.gene83138.t1 MTR_3g087770 61.600 125 46 2 24 147 29 152 1.16e-53 166
MS.gene83138.t1 MTR_3g117890 51.282 156 67 3 1 147 1 156 8.76e-52 162
MS.gene83138.t1 MTR_4g073040 50.641 156 68 3 1 147 1 156 2.92e-50 158
MS.gene83138.t1 MTR_8g091420 59.677 124 48 1 26 147 30 153 8.87e-50 156
MS.gene83138.t1 MTR_5g022620 52.500 160 60 5 1 147 1 157 4.39e-49 155
MS.gene83138.t1 MTR_6g003990 61.864 118 42 2 33 147 1 118 2.13e-48 152
MS.gene83138.t1 MTR_5g055020 45.578 147 80 0 1 147 1 147 1.05e-47 151
MS.gene83138.t1 MTR_3g073670 51.351 148 67 3 2 147 5 149 3.25e-43 140
MS.gene83138.t1 MTR_1g078090 37.313 134 74 2 24 147 52 185 7.92e-28 102
MS.gene83138.t1 MTR_6g026890 35.000 140 69 4 24 147 15 148 6.55e-26 95.9
MS.gene83138.t1 MTR_2g091235 35.374 147 67 4 24 147 1 142 2.33e-23 89.4
MS.gene83138.t1 MTR_6g045203 37.500 136 70 5 27 147 5 140 1.33e-18 77.0
MS.gene83138.t1 MTR_3g010130 67.925 53 16 1 1 53 1 52 4.90e-17 71.2
MS.gene83138.t1 MTR_1g107565 45.455 66 36 0 24 89 12 77 4.93e-14 68.2
MS.gene83138.t1 MTR_2g022250 45.455 66 36 0 24 89 9 74 8.00e-14 67.8
MS.gene83138.t1 MTR_4g117140 43.939 66 37 0 24 89 9 74 2.05e-13 66.6
MS.gene83138.t1 MTR_4g117220 43.939 66 37 0 24 89 9 74 2.88e-13 66.2
MS.gene83138.t1 MTR_7g108560 41.538 65 38 0 25 89 22 86 2.97e-13 65.5
MS.gene83138.t1 MTR_7g013660 43.077 65 37 0 25 89 4 68 6.17e-13 62.0
MS.gene83138.t1 MTR_1g082650 40.909 66 39 0 24 89 9 74 8.05e-13 65.1
MS.gene83138.t1 MTR_1g092670 41.538 65 38 0 25 89 4 68 1.74e-12 59.7
MS.gene83138.t1 MTR_4g094238 35.294 85 52 2 24 108 1 82 1.95e-12 59.7
MS.gene83138.t1 MTR_7g113110 43.548 62 35 0 29 90 37 98 3.28e-12 63.2
MS.gene83138.t1 MTR_1g083310 35.616 73 46 1 19 90 9 81 9.53e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83138.t1 AT1G22990 59.868 152 56 3 1 147 1 152 5.72e-61 185
MS.gene83138.t1 AT1G71050 59.211 152 57 2 1 147 1 152 2.11e-58 178
MS.gene83138.t1 AT1G22990 55.405 148 64 2 1 147 1 147 1.02e-56 174
MS.gene83138.t1 AT5G17450 55.705 149 64 2 1 147 1 149 1.56e-52 163
MS.gene83138.t1 AT4G39700 54.717 159 59 3 1 147 1 158 7.76e-48 152
MS.gene83138.t1 AT4G38580 49.673 153 71 3 1 147 1 153 2.21e-47 150
MS.gene83138.t1 AT4G38580 49.673 153 71 3 1 147 1 153 2.21e-47 150
MS.gene83138.t1 AT4G35060 44.444 153 79 2 1 147 1 153 1.27e-46 149
MS.gene83138.t1 AT5G66110 51.429 140 62 2 14 147 8 147 6.08e-45 144
MS.gene83138.t1 AT5G66110 51.429 140 62 2 14 147 8 147 6.08e-45 144
MS.gene83138.t1 AT4G08570 51.969 127 57 3 24 147 25 150 5.91e-41 134
MS.gene83138.t1 AT5G66110 51.240 121 53 2 33 147 1 121 4.27e-40 131
MS.gene83138.t1 AT5G17450 56.122 98 42 1 51 147 8 105 1.63e-36 121
MS.gene83138.t1 AT1G06330 37.013 154 63 4 24 147 1 150 6.06e-27 98.6
MS.gene83138.t1 AT2G18196 37.589 141 64 4 24 147 45 178 1.34e-26 98.6
MS.gene83138.t1 AT4G10465 37.410 139 77 2 19 147 45 183 1.60e-26 98.6
MS.gene83138.t1 AT3G56891 36.774 155 64 6 24 147 15 166 6.37e-24 91.7
MS.gene83138.t1 AT2G18196 37.121 132 59 4 33 147 1 125 2.41e-22 86.3
MS.gene83138.t1 AT3G56891 36.986 146 58 6 33 147 1 143 8.45e-22 85.5
MS.gene83138.t1 AT1G29100 33.103 145 73 3 26 147 4 147 3.02e-17 73.6
MS.gene83138.t1 AT1G29100 32.394 142 72 3 29 147 1 141 7.24e-16 70.1
MS.gene83138.t1 AT3G48970 39.416 137 68 6 26 147 4 140 1.91e-14 66.2
MS.gene83138.t1 AT3G48970 39.416 137 68 6 26 147 2 138 1.97e-14 66.2
MS.gene83138.t1 AT3G48970 39.416 137 68 6 26 147 2 138 1.97e-14 66.2
MS.gene83138.t1 AT1G66240 43.077 65 37 0 25 89 33 97 4.00e-13 62.0
MS.gene83138.t1 AT3G06130 42.424 66 38 0 24 89 9 74 6.06e-13 65.1
MS.gene83138.t1 AT3G06130 42.424 66 38 0 24 89 9 74 9.27e-13 64.7
MS.gene83138.t1 AT5G19090 42.424 66 38 0 24 89 9 74 1.57e-12 64.3
MS.gene83138.t1 AT5G19090 42.424 66 38 0 24 89 9 74 1.57e-12 64.3
MS.gene83138.t1 AT5G19090 42.424 66 38 0 24 89 9 74 1.57e-12 64.3
MS.gene83138.t1 AT5G19090 42.424 66 38 0 24 89 9 74 1.80e-12 63.9
MS.gene83138.t1 AT5G19090 42.424 66 38 0 24 89 9 74 1.80e-12 63.9
MS.gene83138.t1 AT3G56240 40.909 66 39 0 25 90 3 68 4.23e-12 59.7
MS.gene83138.t1 AT3G56240 40.909 66 39 0 25 90 3 68 4.23e-12 59.7
MS.gene83138.t1 AT5G27690 40.323 62 37 0 29 90 34 95 6.47e-12 62.0
MS.gene83138.t1 AT1G23000 37.879 66 41 0 24 89 72 137 2.33e-11 60.8
MS.gene83138.t1 AT1G23000 37.143 70 44 0 24 93 12 81 3.20e-11 60.1
MS.gene83138.t1 AT1G56210 43.077 65 37 0 29 93 44 108 9.20e-11 58.9

Find 26 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCAGTTGATGGGTATTC+AGG 0.224406 1.2:-16114430 None:intergenic
AGTTTCTCATCCATTGCATT+AGG 0.260018 1.2:-16116822 None:intergenic
TCATATGCTTGTGATATATA+AGG 0.307329 1.2:-16116747 None:intergenic
ACCAAGTTGTATGGAATATA+TGG 0.307606 1.2:-16116717 None:intergenic
GGTTGAAATCAAGGTGAAAA+TGG 0.336258 1.2:+16114580 MS.gene83138:CDS
ACATGATGGAACATGCATTA+GGG 0.371774 1.2:-16116869 None:intergenic
TTCCCTGAATACCCATCAAC+TGG 0.374593 1.2:+16114427 MS.gene83138:CDS
TACATGATGGAACATGCATT+AGG 0.388858 1.2:-16116870 None:intergenic
GTTTCTCATCCATTGCATTA+GGG 0.401204 1.2:-16116821 None:intergenic
CAAAGCAAAGTAACTGTTAG+TGG 0.418002 1.2:+16116630 MS.gene83138:CDS
CTTGCAGACGGTTGAAATCA+AGG 0.426981 1.2:+16114571 MS.gene83138:intron
GGATAAGCCACCAAGTTGTA+TGG 0.438268 1.2:-16116726 None:intergenic
TTCCAGTTGATGGGTATTCA+GGG 0.463226 1.2:-16114429 None:intergenic
ATTAATGCATTGTTACATGA+TGG 0.474887 1.2:-16116883 None:intergenic
AATTCTGTTGCTTACATGAA+AGG 0.476868 1.2:+16114632 MS.gene83138:CDS
GCCATATATTCCATACAACT+TGG 0.516330 1.2:+16116716 MS.gene83138:CDS
ATGGCTGTGAAAGAAAAGTT+AGG 0.524948 1.2:+16114609 MS.gene83138:CDS
GAACTGATGAAAACCTGCAT+TGG 0.531746 1.2:-16114463 None:intergenic
ATATATTCCATACAACTTGG+TGG 0.560676 1.2:+16116719 MS.gene83138:CDS
GAGTTCTTCACATAACCAGC+TGG 0.565569 1.2:-16116780 None:intergenic
TATGACAAGAAAGCGCCAGC+TGG 0.576330 1.2:+16116765 MS.gene83138:CDS
AACATCTAGCCCTAATGCAA+TGG 0.594396 1.2:+16116812 MS.gene83138:CDS
TTAAAGAAAATACAGAACAC+TGG 0.609096 1.2:+16116675 MS.gene83138:CDS
GCATGCAGGTGTGAAGCAAG+TGG 0.652347 1.2:+16116593 MS.gene83138:intron
AAGGTGAAAATGGACTGTGA+TGG 0.723533 1.2:+16114590 MS.gene83138:CDS
TGCAGGTGTGAAGCAAGTGG+AGG 0.726031 1.2:+16116596 MS.gene83138:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGTTTTATTGTAAATAAA+TGG + chr1.2:16116015-16116034 MS.gene83138:intron 10.0%
!!! TTTTAAGAATTTTTAATAAG+TGG - chr1.2:16116110-16116129 None:intergenic 10.0%
!!! TTTTGTTTTTTTTTTTTTGA+AGG + chr1.2:16116351-16116370 MS.gene83138:intron 10.0%
!!! TTTTTTTTTCTTTCTTTTAA+TGG - chr1.2:16115998-16116017 None:intergenic 10.0%
!! ATACATAATGAGATTATATA+AGG - chr1.2:16115569-16115588 None:intergenic 15.0%
!! CTTCAAAAACTAAAAATTTA+AGG - chr1.2:16115529-16115548 None:intergenic 15.0%
!! TACGAATATAATAATTTCAT+AGG + chr1.2:16115706-16115725 MS.gene83138:intron 15.0%
!! TACTTTAAATCTTATACATA+TGG + chr1.2:16116547-16116566 MS.gene83138:intron 15.0%
!!! AACTACTTTTGAATAATAAA+TGG - chr1.2:16116472-16116491 None:intergenic 15.0%
!!! GTGTTTTGAAAAAAAATTTA+AGG - chr1.2:16115495-16115514 None:intergenic 15.0%
!!! TTTGTGAAGTTATTTTTAAA+AGG + chr1.2:16116193-16116212 MS.gene83138:intron 15.0%
!!! TTTTATTGAAAAAATCTCTA+AGG - chr1.2:16116071-16116090 None:intergenic 15.0%
!!! TTTTGAAAAAAAATTTAAGG+AGG - chr1.2:16115492-16115511 None:intergenic 15.0%
!! AAAAATTATAAGTCTGAGTT+TGG - chr1.2:16115386-16115405 None:intergenic 20.0%
!! AATAAATGCAACATAATACT+AGG + chr1.2:16115870-16115889 MS.gene83138:intron 20.0%
!! ATATTAAGAATGACACATTT+CGG - chr1.2:16115445-16115464 None:intergenic 20.0%
!! ATGTTATGTTATAAACAAAG+AGG - chr1.2:16114525-16114544 None:intergenic 20.0%
!! CAAAAACTAAAAATTTAAGG+AGG - chr1.2:16115526-16115545 None:intergenic 20.0%
!! GAATAAATACTAAAAGAGTT+TGG - chr1.2:16116232-16116251 None:intergenic 20.0%
!! TATAATAATTTCATAGGACT+TGG + chr1.2:16115712-16115731 MS.gene83138:intron 20.0%
!!! TAACGCTATAATTTTTGTTT+CGG - chr1.2:16115742-16115761 None:intergenic 20.0%
!!! TTTTCTTTAACACTCTATTT+CGG - chr1.2:16116665-16116684 None:intergenic 20.0%
!!! TTTTTAGTTTTTGAAGATTG+TGG + chr1.2:16115533-16115552 MS.gene83138:intron 20.0%
! ACAAGAATGAAAACACTAAA+TGG - chr1.2:16115609-16115628 None:intergenic 25.0%
! ATAAGATGTCTCATAAGAAT+AGG + chr1.2:16115782-16115801 MS.gene83138:intron 25.0%
! TATGCTAAACAGACTTTAAA+AGG - chr1.2:16115234-16115253 None:intergenic 25.0%
! TATGGAATCTAATCCATATT+TGG + chr1.2:16116565-16116584 MS.gene83138:intron 25.0%
! TATTAAGAATGACACATTTC+GGG - chr1.2:16115444-16115463 None:intergenic 25.0%
! TCATATGCTTGTGATATATA+AGG - chr1.2:16116750-16116769 None:intergenic 25.0%
! TTAAAGAAAATACAGAACAC+TGG + chr1.2:16116675-16116694 MS.gene83138:CDS 25.0%
! TTTATTTGTTTGAGTATTCG+TGG + chr1.2:16116283-16116302 MS.gene83138:intron 25.0%
!! AAAGAGGTATGAACAAAATT+TGG - chr1.2:16114509-16114528 None:intergenic 25.0%
!! ATAATATGCTTTGTTGCTAT+AGG + chr1.2:16115040-16115059 MS.gene83138:intron 25.0%
!! GGCTTTAATGTTGTTAAAAT+AGG + chr1.2:16115061-16115080 MS.gene83138:intron 25.0%
!! TCTGAAACTTTGCAATTAAT+AGG + chr1.2:16114930-16114949 MS.gene83138:intron 25.0%
!!! AACGCTATAATTTTTGTTTC+GGG - chr1.2:16115741-16115760 None:intergenic 25.0%
!!! GTGAAGTTATTTTTAAAAGG+CGG + chr1.2:16116196-16116215 MS.gene83138:intron 25.0%
!!! TGGTTTTTTCTTGACATAAA+TGG + chr1.2:16114709-16114728 MS.gene83138:intron 25.0%
AAATACACCTAACATTAGCA+TGG + chr1.2:16115683-16115702 MS.gene83138:intron 30.0%
AATGTTAGGTGTATTTGGAT+TGG - chr1.2:16115679-16115698 None:intergenic 30.0%
ACAATTGCATACTTCAAACT+TGG + chr1.2:16114686-16114705 MS.gene83138:intron 30.0%
ACCAAGTTGTATGGAATATA+TGG - chr1.2:16116720-16116739 None:intergenic 30.0%
ACCTTAATGTAAAGTAAGCT+TGG + chr1.2:16115282-16115301 MS.gene83138:intron 30.0%
AGAATGAAAACACTAAATGG+AGG - chr1.2:16115606-16115625 None:intergenic 30.0%
AGCTACGTTGAAAAAAGAAA+TGG + chr1.2:16115907-16115926 MS.gene83138:intron 30.0%
ATATATTCCATACAACTTGG+TGG + chr1.2:16116719-16116738 MS.gene83138:CDS 30.0%
ATGCTAATGTTAGGTGTATT+TGG - chr1.2:16115684-16115703 None:intergenic 30.0%
ATTAAGAATGACACATTTCG+GGG - chr1.2:16115443-16115462 None:intergenic 30.0%
GGTATGAACAAAATTTGGAT+AGG - chr1.2:16114504-16114523 None:intergenic 30.0%
GTCTGACATATTTAAAAGCT+TGG - chr1.2:16115328-16115347 None:intergenic 30.0%
GTCTGTTTAGCATATTTAGT+AGG + chr1.2:16115240-16115259 MS.gene83138:intron 30.0%
TCCAAGCTTACTTTACATTA+AGG - chr1.2:16115286-16115305 None:intergenic 30.0%
TTGTATATTTCTCTTGCAGA+CGG + chr1.2:16114559-16114578 MS.gene83138:intron 30.0%
! AATTCTGTTGCTTACATGAA+AGG + chr1.2:16114632-16114651 MS.gene83138:CDS 30.0%
! GTTTTCATCACTAAACATGT+TGG - chr1.2:16116850-16116869 None:intergenic 30.0%
!! CAGTATGAAAAGCATTAATG+AGG - chr1.2:16115656-16115675 None:intergenic 30.0%
AAAAAGACACAAACCAATGC+AGG + chr1.2:16114450-16114469 MS.gene83138:CDS 35.0%
ACATTGGCATGAATCATGTA+TGG - chr1.2:16115969-16115988 None:intergenic 35.0%
AGTTTCTCATCCATTGCATT+AGG - chr1.2:16116825-16116844 None:intergenic 35.0%
ATGATTCATGCCAATGTTCT+TGG + chr1.2:16115972-16115991 MS.gene83138:intron 35.0%
ATGGCTGTGAAAGAAAAGTT+AGG + chr1.2:16114609-16114628 MS.gene83138:CDS 35.0%
ATTCGTACCATGCTAATGTT+AGG - chr1.2:16115693-16115712 None:intergenic 35.0%
ATTGCATACTTCAAACTTGG+TGG + chr1.2:16114689-16114708 MS.gene83138:intron 35.0%
CAAAGCAAAGTAACTGTTAG+TGG + chr1.2:16116630-16116649 MS.gene83138:CDS 35.0%
CAACGTAGCTAAGTAAGTAA+AGG - chr1.2:16115897-16115916 None:intergenic 35.0%
GCCATATATTCCATACAACT+TGG + chr1.2:16116716-16116735 MS.gene83138:CDS 35.0%
GGTTGAAATCAAGGTGAAAA+TGG + chr1.2:16114580-16114599 MS.gene83138:CDS 35.0%
GTTTCTCATCCATTGCATTA+GGG - chr1.2:16116824-16116843 None:intergenic 35.0%
TCAGACTAGACCAAGAAAAA+CGG + chr1.2:16115342-16115361 MS.gene83138:intron 35.0%
TGTAAAGTAAGCTTGGAAGT+AGG + chr1.2:16115289-16115308 MS.gene83138:intron 35.0%
TTTGAGTATTCGTGGAGAAA+CGG + chr1.2:16116291-16116310 MS.gene83138:intron 35.0%
TTTGGCATATGACCTATCAT+AGG - chr1.2:16115368-16115387 None:intergenic 35.0%
! ACATGATGGAACATGCATTA+GGG - chr1.2:16116872-16116891 None:intergenic 35.0%
! CTTTTAATGGCCAAGAACAT+TGG - chr1.2:16115985-16116004 None:intergenic 35.0%
! TACATGATGGAACATGCATT+AGG - chr1.2:16116873-16116892 None:intergenic 35.0%
! TCTTTTTGTCCTTGAGTGAT+TGG - chr1.2:16115844-16115863 None:intergenic 35.0%
! TGTGTCTTTTTCCAGTTGAT+GGG - chr1.2:16114441-16114460 None:intergenic 35.0%
! TTGTGTCTTTTTCCAGTTGA+TGG - chr1.2:16114442-16114461 None:intergenic 35.0%
!! TTTTCATTCTTGTTTGCGAG+AGG + chr1.2:16115616-16115635 MS.gene83138:intron 35.0%
AACATCTAGCCCTAATGCAA+TGG + chr1.2:16116812-16116831 MS.gene83138:CDS 40.0%
AGACTTGAACCCAAGTCATA+CGG - chr1.2:16114913-16114932 None:intergenic 40.0%
CAAGAAAAACGGCCTATGAT+AGG + chr1.2:16115353-16115372 MS.gene83138:intron 40.0%
GAACTGATGAAAACCTGCAT+TGG - chr1.2:16114466-16114485 None:intergenic 40.0%
GACTCATGCCTTTCAAAACT+TGG + chr1.2:16115417-16115436 MS.gene83138:intron 40.0%
GTTAAATGCCCGTATGACTT+GGG + chr1.2:16114901-16114920 MS.gene83138:intron 40.0%
TCTTGAACACCAATCACTCA+AGG + chr1.2:16115832-16115851 MS.gene83138:intron 40.0%
! CTGGAAAAAGAGCAGAGTTT+TGG + chr1.2:16116694-16116713 MS.gene83138:CDS 40.0%
!! AAGGTGAAAATGGACTGTGA+TGG + chr1.2:16114590-16114609 MS.gene83138:CDS 40.0%
!! TTCCAGTTGATGGGTATTCA+GGG - chr1.2:16114432-16114451 None:intergenic 40.0%
!! TTTCCAGTTGATGGGTATTC+AGG - chr1.2:16114433-16114452 None:intergenic 40.0%
!!! AGTTTTGAAAGGCATGAGTC+TGG - chr1.2:16115417-16115436 None:intergenic 40.0%
!!! CTATCATAGGCCGTTTTTCT+TGG - chr1.2:16115355-16115374 None:intergenic 40.0%
!!! GGCTCGGAGTTTTGAAATTT+TGG - chr1.2:16114845-16114864 None:intergenic 40.0%
CCAATTGCGGTTGACATAGA+TGG + chr1.2:16114867-16114886 MS.gene83138:intron 45.0%
CCATCTATGTCAACCGCAAT+TGG - chr1.2:16114870-16114889 None:intergenic 45.0%
CGTTAAATGCCCGTATGACT+TGG + chr1.2:16114900-16114919 MS.gene83138:intron 45.0%
CTATGTCAACCGCAATTGGT+GGG - chr1.2:16114866-16114885 None:intergenic 45.0%
CTTGCAGACGGTTGAAATCA+AGG + chr1.2:16114571-16114590 MS.gene83138:intron 45.0%
GACTTGAACCCAAGTCATAC+GGG - chr1.2:16114912-16114931 None:intergenic 45.0%
GAGTTCTTCACATAACCAGC+TGG - chr1.2:16116783-16116802 None:intergenic 45.0%
GGATAAGCCACCAAGTTGTA+TGG - chr1.2:16116729-16116748 None:intergenic 45.0%
TCTATGTCAACCGCAATTGG+TGG - chr1.2:16114867-16114886 None:intergenic 45.0%
TTCCCTGAATACCCATCAAC+TGG + chr1.2:16114427-16114446 MS.gene83138:CDS 45.0%
! TCATTCTTGTTTGCGAGAGG+TGG + chr1.2:16115619-16115638 MS.gene83138:intron 45.0%
!!! TTTCGGGGCCAAGTTTTGAA+AGG - chr1.2:16115428-16115447 None:intergenic 45.0%
CATATTTGGCCCATGCATGC+AGG + chr1.2:16116579-16116598 MS.gene83138:intron 50.0%
CTGCATGCATGGGCCAAATA+TGG - chr1.2:16116581-16116600 None:intergenic 50.0%
TGCTTCACACCTGCATGCAT+GGG - chr1.2:16116591-16116610 None:intergenic 50.0%
TGTTTGCGAGAGGTGGAAAG+TGG + chr1.2:16115626-16115645 MS.gene83138:intron 50.0%
TTGCTTCACACCTGCATGCA+TGG - chr1.2:16116592-16116611 None:intergenic 50.0%
!! TATGACAAGAAAGCGCCAGC+TGG + chr1.2:16116765-16116784 MS.gene83138:CDS 50.0%
AACTCCGAGCCCACCAATTG+CGG + chr1.2:16114854-16114873 MS.gene83138:intron 55.0%
GCATGCAGGTGTGAAGCAAG+TGG + chr1.2:16116593-16116612 MS.gene83138:intron 55.0%
TGCAGGTGTGAAGCAAGTGG+AGG + chr1.2:16116596-16116615 MS.gene83138:intron 55.0%
!! TCAACCGCAATTGGTGGGCT+CGG - chr1.2:16114861-16114880 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 16114397 16116893 16114397 ID=MS.gene83138
chr1.2 mRNA 16114397 16116893 16114397 ID=MS.gene83138.t1;Parent=MS.gene83138
chr1.2 exon 16114397 16114471 16114397 ID=MS.gene83138.t1.exon1;Parent=MS.gene83138.t1
chr1.2 CDS 16114397 16114471 16114397 ID=cds.MS.gene83138.t1;Parent=MS.gene83138.t1
chr1.2 exon 16114578 16114653 16114578 ID=MS.gene83138.t1.exon2;Parent=MS.gene83138.t1
chr1.2 CDS 16114578 16114653 16114578 ID=cds.MS.gene83138.t1;Parent=MS.gene83138.t1
chr1.2 exon 16116601 16116893 16116601 ID=MS.gene83138.t1.exon3;Parent=MS.gene83138.t1
chr1.2 CDS 16116601 16116893 16116601 ID=cds.MS.gene83138.t1;Parent=MS.gene83138.t1
Gene Sequence

>MS.gene83138

ATGGGTGTTCTTGATAAACTATCTGATTATTTCCCTGAATACCCATCAACTGGAAAAAGACACAAACCAATGCAGGTTTTCATCAGTTCTTAGTCTTACTCTTACCTATCCAAATTTTGTTCATACCTCTTTGTTTATAACATAACATGATTTTGATATTCTTTGTATATTTCTCTTGCAGACGGTTGAAATCAAGGTGAAAATGGACTGTGATGGCTGTGAAAGAAAAGTTAGGAATTCTGTTGCTTACATGAAAGGTTTATATTTTTCTTTTTCTTGCTCTATAGTAACAATTGCATACTTCAAACTTGGTGGTTTTTTCTTGACATAAATGGAAAAAACTATATTATTTTCATCTTTTAATAAACTTTACTTTCATTTTATGTTTTGTCTTACTCTTACTTCAGTATTTACATTCTTTTTTTCTTTGAGACTACTTTTTGATCCAAAATTTCAAAACTCCGAGCCCACCAATTGCGGTTGACATAGATGGTTGAACATTACGTTAAATGCCCGTATGACTTGGGTTCAAGTCTGAAACTTTGCAATTAATAGGTTATACGTAAAAAAAAAGTATTAAAAAGTATTTTTTATATTAAAATAATTGCATGCATTAAAAATATAAACACATTTTTCTCTATCTATAATATGCTTTGTTGCTATAGGCTTTAATGTTGTTAAAATAGGTTATACGTAAAAAAAAAGTATTAAAAAGTATTTTTTTATATTAAAATAATTGCATGCATTAAAAATATAAACACATTTTTCTCTATCTATAATATGCTTTGTTGCTATAGGCTTTAATGTTGTTAAAATTTGTTTGTAGTGAAACAGCCTTTTAAAGTCTGTTTAGCATATTTAGTAGGTCAAATGAACTATTTGATGACCTTAATGTAAAGTAAGCTTGGAAGTAGGCTTTCAAGTCAGTCCAAGCTTTTAAATATGTCAGACTAGACCAAGAAAAACGGCCTATGATAGGTCATATGCCAAACTCAGACTTATAATTTTTTTTTTAGACCAGACTCATGCCTTTCAAAACTTGGCCCCGAAATGTGTCATTCTTAATATACATTTTTTAAAGCACTCTTTGTACCTCCTTAAATTTTTTTTCAAAACACTCTTTGTACCTCCTTAAATTTTTAGTTTTTGAAGATTGTGGATTGATTCTTCCTTATATAATCTCATTATGTATTGTCAAATGAGCTACCTCCATTTAGTGTTTTCATTCTTGTTTGCGAGAGGTGGAAAGTGGAAAACCTCATTAATGCTTTTCATACTGCCAATCCAAATACACCTAACATTAGCATGGTACGAATATAATAATTTCATAGGACTTGGTTGCCCGAAACAAAAATTATAGCGTTACTGTAATTGTTGTACAAAATATAAGATGTCTCATAAGAATAGGTTGAGCAATAAATTTTTCAACTCTTTCTCTTGAACACCAATCACTCAAGGACAAAAAGAAAACATAATAAATGCAACATAATACTAGGTCCTTTACTTACTTAGCTACGTTGAAAAAAGAAATGGTTCTTTTGTTCACATGCTTCGTGTGAACATTCGAATCCATACATGATTCATGCCAATGTTCTTGGCCATTAAAAGAAAGAAAAAAAAAGTTTTATTGTAAATAAATGGAGCTAAAATAAAGTTTTTATTAGCTAATAACCTTAGAGATTTTTTCAATAAAAAAAAGTTTATTTTTCACCACTTATTAAAAATTCTTAAAATCTTAAAAAGAAATATAAAGAGAGCAAAGTAAAAATTGAGATATGAAAGCTAATTTCAGCTTTTTTGTGAAGTTATTTTTAAAAGGCGGTTAAGACTTTCCAAACTCTTTTAGTATTTATTCTTAAAAAAAAAAAAAAAAACTCTTTTAGTATTTTATTTGTTTGAGTATTCGTGGAGAAACGGTTGATTATGTGATTACAAAAATTAATTTTAATTGAAATTTTGTTTTTTTTTTTTTGAAGGAATTGAAATTTGTTAAATGTATATCGATTTACAATTAGATATATTTAAATTGCATTTGAAATCAGAAACTTTAAGTCTAGACTTCGAGTGAGAATCCATTTATTATTCAAAAGTAGTTCTATTAAAAAAAAAACACTTTAAACATATTAAAATCAATTTTATTTGTTTAAAGCTACTTTAAATCTTATACATATGGAATCTAATCCATATTTGGCCCATGCATGCAGGTGTGAAGCAAGTGGAGGTAAATAGAAAGCAAAGCAAAGTAACTGTTAGTGGTAATGTTGACCGAAATAGAGTGTTAAAGAAAATACAGAACACTGGAAAAAGAGCAGAGTTTTGGCCATATATTCCATACAACTTGGTGGCTTATCCTTATATATCACAAGCATATGACAAGAAAGCGCCAGCTGGTTATGTGAAGAACTCAATTCAAGCAACATCTAGCCCTAATGCAATGGATGAGAAACTCACCAACATGTTTAGTGATGAAAACCCTAATGCATGTTCCATCATGTAA
Protein sequence

>MS.gene83138.t1

MGVLDKLSDYFPEYPSTGKRHKPMQTVEIKVKMDCDGCERKVRNSVAYMKGVKQVEVNRKQSKVTVSGNVDRNRVLKKIQNTGKRAEFWPYIPYNLVAYPYISQAYDKKAPAGYVKNSIQATSSPNAMDEKLTNMFSDENPNACSIM