Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036595.t1 | NP_001275136.1 | 100 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 8.20E-68 | 266.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036595.t1 | P42798 | 99.2 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 2.6e-70 | 265.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036595.t1 | A0A1U7YEG5 | 100.0 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 5.9e-68 | 266.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene55887 | MS.gene036595 | PPI |
| MS.gene20156 | MS.gene036595 | PPI |
| MS.gene036595 | MS.gene023130 | PPI |
| MS.gene036595 | MS.gene06172 | PPI |
| MS.gene036595 | MS.gene58227 | PPI |
| MS.gene041458 | MS.gene036595 | PPI |
| MS.gene36468 | MS.gene036595 | PPI |
| MS.gene036595 | MS.gene58719 | PPI |
| MS.gene028323 | MS.gene036595 | PPI |
| MS.gene35135 | MS.gene036595 | PPI |
| MS.gene036595 | MS.gene049026 | PPI |
| MS.gene58719 | MS.gene036595 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036595.t1 | MTR_4g117470 | 100.000 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 1.05e-93 | 266 |
| MS.gene036595.t1 | MTR_4g094598 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 2.82e-93 | 265 |
| MS.gene036595.t1 | MTR_1g105415 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 2.82e-93 | 265 |
| MS.gene036595.t1 | MTR_8g101910 | 52.000 | 125 | 60 | 0 | 6 | 130 | 5 | 129 | 1.82e-45 | 144 |
| MS.gene036595.t1 | MTR_8g101910 | 49.474 | 95 | 48 | 0 | 6 | 100 | 5 | 99 | 1.72e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036595.t1 | AT5G59850 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.76e-93 | 265 |
| MS.gene036595.t1 | AT1G07770 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.76e-93 | 265 |
| MS.gene036595.t1 | AT1G07770 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.76e-93 | 265 |
| MS.gene036595.t1 | AT1G07770 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.76e-93 | 265 |
| MS.gene036595.t1 | AT3G46040 | 97.692 | 130 | 3 | 0 | 1 | 130 | 1 | 130 | 4.03e-92 | 262 |
| MS.gene036595.t1 | AT2G39590 | 91.538 | 130 | 11 | 0 | 1 | 130 | 1 | 130 | 4.30e-87 | 249 |
| MS.gene036595.t1 | AT4G29430 | 54.400 | 125 | 57 | 0 | 6 | 130 | 5 | 129 | 2.98e-47 | 149 |
| MS.gene036595.t1 | AT2G19720 | 53.600 | 125 | 58 | 0 | 6 | 130 | 5 | 129 | 3.11e-45 | 144 |
Find 30 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACCACTTCTGCTGGTATTA+TGG | 0.215449 | 8.3:-8749168 | MS.gene036595:CDS |
| TTATGCTTTATAGGTTATAT+TGG | 0.301097 | 8.3:-8750381 | MS.gene036595:intron |
| TATGATTAGGCCTTCCTCTA+AGG | 0.335085 | 8.3:-8750583 | MS.gene036595:CDS |
| GAATTTGATGATAACCTTAG+AGG | 0.407415 | 8.3:+8750569 | None:intergenic |
| GAAAGCGTCAAGTTATGATT+AGG | 0.452540 | 8.3:-8750596 | MS.gene036595:CDS |
| TATGTTGATGATCACAGATC+TGG | 0.452973 | 8.3:-8750348 | MS.gene036595:CDS |
| GGTCCTTGGCTTCTTCTACT+AGG | 0.468896 | 8.3:-8749108 | None:intergenic |
| AATGTTGGTGGTAAGGTCCT+TGG | 0.482167 | 8.3:-8749122 | MS.gene036595:CDS |
| AAAATCGTTGTTGAATTGAA+TGG | 0.485579 | 8.3:-8750324 | MS.gene036595:CDS |
| GAGAAAGAATGTTGGTGGTA+AGG | 0.495772 | 8.3:-8749129 | MS.gene036595:CDS |
| ATGGTAGGTTGAACAAATGT+GGG | 0.519393 | 8.3:-8750305 | MS.gene036595:CDS |
| ATCCATAATACCAGCAGAAG+TGG | 0.531152 | 8.3:+8749166 | None:intergenic |
| TAGACTTCTCCCTTCCAGAC+AGG | 0.537117 | 8.3:-8750229 | MS.gene036595:intron |
| ATGTACAATGCTGAGAAAAG+AGG | 0.544704 | 8.3:-8750618 | MS.gene036595:CDS |
| TTCAACTGACCTGTCTGGAA+GGG | 0.545440 | 8.3:+8750220 | None:intergenic |
| TTTCAACTGACCTGTCTGGA+AGG | 0.549780 | 8.3:+8750219 | None:intergenic |
| ATGTTGATGATCACAGATCT+GGG | 0.565212 | 8.3:-8750347 | MS.gene036595:CDS |
| GCTAGGAGAAAGAATGTTGG+TGG | 0.567141 | 8.3:-8749134 | MS.gene036595:CDS |
| TAGCCTAGTAGAAGAAGCCA+AGG | 0.575489 | 8.3:+8749105 | None:intergenic |
| AATGGTAGGTTGAACAAATG+TGG | 0.577364 | 8.3:-8750306 | MS.gene036595:CDS |
| GAGGCTAGGAGAAAGAATGT+TGG | 0.584494 | 8.3:-8749137 | MS.gene036595:CDS |
| TTATGGATCATGAAGAGGCT+AGG | 0.585160 | 8.3:-8749151 | MS.gene036595:CDS |
| TTGATGATAACCTTAGAGGA+AGG | 0.606187 | 8.3:+8750573 | None:intergenic |
| TGGTATTATGGATCATGAAG+AGG | 0.606467 | 8.3:-8749156 | MS.gene036595:CDS |
| GTCGGTGTCAAGGAGATTGA+AGG | 0.613514 | 8.3:-8750261 | MS.gene036595:CDS |
| TCGTTGTTGAATTGAATGGT+AGG | 0.614641 | 8.3:-8750320 | MS.gene036595:CDS |
| CAAAACACTGACTCTCACCA+TGG | 0.638914 | 8.3:+8750659 | None:intergenic |
| GTGTCAAGGAGATTGAAGGT+TGG | 0.653545 | 8.3:-8750257 | MS.gene036595:CDS |
| TGGTAGGTTGAACAAATGTG+GGG | 0.708928 | 8.3:-8750304 | MS.gene036595:CDS |
| TTGACACCGACATCAAAACG+AGG | 0.734537 | 8.3:+8750273 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTAAATAATAACAATTTA+AGG | + | chr8.3:8750462-8750481 | None:intergenic | 10.0% |
| !! | CTAAAATTGATCATAAAAAA+CGG | + | chr8.3:8750338-8750357 | None:intergenic | 15.0% |
| !! | TAATTATAAAATATGCAGTT+TGG | - | chr8.3:8750563-8750582 | MS.gene036595:CDS | 15.0% |
| !!! | AACTTAATTTATCTTTTGAT+AGG | - | chr8.3:8749747-8749766 | MS.gene036595:intron | 15.0% |
| !!! | TGAAAACAATACTAATTTTT+GGG | - | chr8.3:8749565-8749584 | MS.gene036595:intron | 15.0% |
| !!! | TTGAAAACAATACTAATTTT+TGG | - | chr8.3:8749564-8749583 | MS.gene036595:intron | 15.0% |
| !!! | TTGATTTGATATAATTTTGT+GGG | - | chr8.3:8749310-8749329 | MS.gene036595:intron | 15.0% |
| !!! | TTTGATTTGATATAATTTTG+TGG | - | chr8.3:8749309-8749328 | MS.gene036595:intron | 15.0% |
| !!! | TTTGATTTGATATATTTTGT+GGG | - | chr8.3:8749362-8749381 | MS.gene036595:intron | 15.0% |
| !!! | TTTTGATTTGATATATTTTG+TGG | - | chr8.3:8749361-8749380 | MS.gene036595:intron | 15.0% |
| !! | AAAACTAATAAATGTTGCAT+TGG | + | chr8.3:8749858-8749877 | None:intergenic | 20.0% |
| !! | GGATGAAATTCATTAAATAA+GGG | + | chr8.3:8749894-8749913 | None:intergenic | 20.0% |
| !! | TGGATGAAATTCATTAAATA+AGG | + | chr8.3:8749895-8749914 | None:intergenic | 20.0% |
| !! | TTATGCTTTATAGGTTATAT+TGG | - | chr8.3:8749385-8749404 | MS.gene036595:intron | 20.0% |
| !! | TTGCATGATTCAATTATTTA+GGG | - | chr8.3:8749285-8749304 | MS.gene036595:intron | 20.0% |
| !!! | AAAAACGGAGATTTTTTTTT+AGG | + | chr8.3:8750323-8750342 | None:intergenic | 20.0% |
| !!! | AGGAAAAAAAAAGATTTTAG+AGG | + | chr8.3:8749254-8749273 | None:intergenic | 20.0% |
| !!! | ATTAAATAAGGGTATAATAG+TGG | + | chr8.3:8749883-8749902 | None:intergenic | 20.0% |
| !!! | ATTAATTGTGTTGATTTCAA+TGG | + | chr8.3:8749978-8749997 | None:intergenic | 20.0% |
| !!! | TTTTTTTTTCCTTTCATTGA+AGG | - | chr8.3:8749262-8749281 | MS.gene036595:intron | 20.0% |
| ! | AAAATCGTTGTTGAATTGAA+TGG | - | chr8.3:8749442-8749461 | MS.gene036595:intron | 25.0% |
| ! | ATTGAACTTATATGAACGTT+GGG | - | chr8.3:8750488-8750507 | MS.gene036595:intron | 25.0% |
| ! | GTTGCATGATTCAATTATTT+AGG | - | chr8.3:8749284-8749303 | MS.gene036595:intron | 25.0% |
| ! | TATAGTTTCTTACTCCTATT+TGG | - | chr8.3:8750268-8750287 | MS.gene036595:CDS | 25.0% |
| ! | TATTGAACTTATATGAACGT+TGG | - | chr8.3:8750487-8750506 | MS.gene036595:intron | 25.0% |
| ! | TCATGCTTCATATTAAAGAT+TGG | - | chr8.3:8750232-8750251 | MS.gene036595:CDS | 25.0% |
| !! | TATAATTTTGTGGGTATGTT+TGG | - | chr8.3:8749319-8749338 | MS.gene036595:intron | 25.0% |
| !! | TGATTTGGAACAAATTTACT+CGG | + | chr8.3:8750205-8750224 | None:intergenic | 25.0% |
| !!! | TCAGCTTATTTTCATAAACA+TGG | - | chr8.3:8749698-8749717 | MS.gene036595:intron | 25.0% |
| !!! | TTTGGTGGTTCAATTTTTTA+GGG | - | chr8.3:8749337-8749356 | MS.gene036595:intron | 25.0% |
| AAACAACACATACACACAAA+AGG | + | chr8.3:8750519-8750538 | None:intergenic | 30.0% | |
| AAACGACTAATATTGTGAGA+CGG | - | chr8.3:8750027-8750046 | MS.gene036595:intron | 30.0% | |
| AAGCATGAACTATCATGATT+TGG | + | chr8.3:8750220-8750239 | None:intergenic | 30.0% | |
| AAGTTCACACAAATATGCAT+TGG | + | chr8.3:8750132-8750151 | None:intergenic | 30.0% | |
| ACACCCTTATTAATTGTAGT+TGG | + | chr8.3:8749932-8749951 | None:intergenic | 30.0% | |
| ATTTACTCGGCTTAACAAAA+TGG | + | chr8.3:8750192-8750211 | None:intergenic | 30.0% | |
| CATAGCTATATGTAAGAACA+TGG | + | chr8.3:8750093-8750112 | None:intergenic | 30.0% | |
| CCACCAACTACAATTAATAA+GGG | - | chr8.3:8749926-8749945 | MS.gene036595:intron | 30.0% | |
| CTCACAATATTAGTCGTTTA+AGG | + | chr8.3:8750026-8750045 | None:intergenic | 30.0% | |
| TCCACCAACTACAATTAATA+AGG | - | chr8.3:8749925-8749944 | MS.gene036595:intron | 30.0% | |
| TTTGGGAGAACTTATGAAAA+CGG | - | chr8.3:8749650-8749669 | MS.gene036595:intron | 30.0% | |
| ! | GAATTTGATGATAACCTTAG+AGG | + | chr8.3:8749200-8749219 | None:intergenic | 30.0% |
| !! | TTTTGTGGGTTATGCTTTAT+AGG | - | chr8.3:8749376-8749395 | MS.gene036595:intron | 30.0% |
| !!! | GTTTGGTGGTTCAATTTTTT+AGG | - | chr8.3:8749336-8749355 | MS.gene036595:intron | 30.0% |
| ACACAAATATGCATTGGAAG+TGG | + | chr8.3:8750126-8750145 | None:intergenic | 35.0% | |
| AGACAATAATTGTGAGACAG+AGG | + | chr8.3:8749789-8749808 | None:intergenic | 35.0% | |
| AGGTTTGTGCACGATTATTA+AGG | + | chr8.3:8750006-8750025 | None:intergenic | 35.0% | |
| AGTAAGCTGTTTATCCAAAC+AGG | - | chr8.3:8750066-8750085 | MS.gene036595:intron | 35.0% | |
| ATCATGCAACCTTCAATGAA+AGG | + | chr8.3:8749274-8749293 | None:intergenic | 35.0% | |
| ATGTACAATGCTGAGAAAAG+AGG | - | chr8.3:8749148-8749167 | MS.gene036595:CDS | 35.0% | |
| CCCTTATTAATTGTAGTTGG+TGG | + | chr8.3:8749929-8749948 | None:intergenic | 35.0% | |
| CTCATCGTTATGCAAAAACA+CGG | - | chr8.3:8749220-8749239 | MS.gene036595:intron | 35.0% | |
| GACAATAATTGTGAGACAGA+GGG | + | chr8.3:8749788-8749807 | None:intergenic | 35.0% | |
| TATCACCTGTGAGAATGTTT+GGG | - | chr8.3:8749633-8749652 | MS.gene036595:intron | 35.0% | |
| TCGTTGTTGAATTGAATGGT+AGG | - | chr8.3:8749446-8749465 | MS.gene036595:intron | 35.0% | |
| TGGTATTATGGATCATGAAG+AGG | - | chr8.3:8750610-8750629 | MS.gene036595:CDS | 35.0% | |
| TTATCACCTGTGAGAATGTT+TGG | - | chr8.3:8749632-8749651 | MS.gene036595:intron | 35.0% | |
| TTGTTCCACAAAGACCAAAT+AGG | + | chr8.3:8750285-8750304 | None:intergenic | 35.0% | |
| ! | AATGGTAGGTTGAACAAATG+TGG | - | chr8.3:8749460-8749479 | MS.gene036595:intron | 35.0% |
| ! | AATTTTGTGGGTATGTTTGG+TGG | - | chr8.3:8749322-8749341 | MS.gene036595:intron | 35.0% |
| ! | ATGGTAGGTTGAACAAATGT+GGG | - | chr8.3:8749461-8749480 | MS.gene036595:intron | 35.0% |
| ! | ATTTGTTGCTTGGATGATCT+AGG | - | chr8.3:8750410-8750429 | MS.gene036595:intron | 35.0% |
| ! | TTACTCCTATTTGGTCTTTG+TGG | - | chr8.3:8750277-8750296 | MS.gene036595:CDS | 35.0% |
| ! | TTGATGATAACCTTAGAGGA+AGG | + | chr8.3:8749196-8749215 | None:intergenic | 35.0% |
| !! | ATGTTGATGATCACAGATCT+GGG | - | chr8.3:8749419-8749438 | MS.gene036595:intron | 35.0% |
| !! | ATTAGTCCTCGTTTTGATGT+CGG | - | chr8.3:8749487-8749506 | MS.gene036595:intron | 35.0% |
| !! | GAAAGCGTCAAGTTATGATT+AGG | - | chr8.3:8749170-8749189 | MS.gene036595:CDS | 35.0% |
| !! | TATGTTGATGATCACAGATC+TGG | - | chr8.3:8749418-8749437 | MS.gene036595:intron | 35.0% |
| !! | TCAATTTTAGCCTCTACTAG+AGG | - | chr8.3:8750348-8750367 | MS.gene036595:CDS | 35.0% |
| !! | TCTGTCAACAATTTGTTGCT+TGG | - | chr8.3:8750400-8750419 | MS.gene036595:intron | 35.0% |
| ATCCATAATACCAGCAGAAG+TGG | + | chr8.3:8750603-8750622 | None:intergenic | 40.0% | |
| CACATAAAAGCCTCTAGTAG+AGG | + | chr8.3:8750361-8750380 | None:intergenic | 40.0% | |
| GACTAATATTGTGAGACGGA+GGG | - | chr8.3:8750031-8750050 | MS.gene036595:intron | 40.0% | |
| TATGATTAGGCCTTCCTCTA+AGG | - | chr8.3:8749183-8749202 | MS.gene036595:CDS | 40.0% | |
| TGTAAGAACATGGTCCTGTT+TGG | + | chr8.3:8750083-8750102 | None:intergenic | 40.0% | |
| TTATGGATCATGAAGAGGCT+AGG | - | chr8.3:8750615-8750634 | MS.gene036595:CDS | 40.0% | |
| ! | CTACTAGAGGCTTTTATGTG+TGG | - | chr8.3:8750361-8750380 | MS.gene036595:CDS | 40.0% |
| ! | CTTCAGTGTTATCGATTGCA+TGG | - | chr8.3:8750163-8750182 | MS.gene036595:intron | 40.0% |
| ! | TGGTAGGTTGAACAAATGTG+GGG | - | chr8.3:8749462-8749481 | MS.gene036595:intron | 40.0% |
| ! | TTCTTTTGCATAGTGTCTGC+TGG | - | chr8.3:8749592-8749611 | MS.gene036595:intron | 40.0% |
| ! | TTGTTTTCAACTGACCTGTC+TGG | + | chr8.3:8749554-8749573 | None:intergenic | 40.0% |
| !! | AGTTGGTGGAGTAGTTAAGT+TGG | + | chr8.3:8749915-8749934 | None:intergenic | 40.0% |
| !! | CGTGTTTTTGCATAACGATG+AGG | + | chr8.3:8749222-8749241 | None:intergenic | 40.0% |
| !! | GAGAAAGAATGTTGGTGGTA+AGG | - | chr8.3:8750637-8750656 | MS.gene036595:CDS | 40.0% |
| !!! | ATTGTTTTGACCACTTCTGC+TGG | - | chr8.3:8750590-8750609 | MS.gene036595:CDS | 40.0% |
| CGACTAATATTGTGAGACGG+AGG | - | chr8.3:8750030-8750049 | MS.gene036595:intron | 45.0% | |
| GACCACTTCTGCTGGTATTA+TGG | - | chr8.3:8750598-8750617 | MS.gene036595:CDS | 45.0% | |
| GAGGCTAGGAGAAAGAATGT+TGG | - | chr8.3:8750629-8750648 | MS.gene036595:CDS | 45.0% | |
| GCTAGGAGAAAGAATGTTGG+TGG | - | chr8.3:8750632-8750651 | MS.gene036595:CDS | 45.0% | |
| GTGTCAAGGAGATTGAAGGT+TGG | - | chr8.3:8749509-8749528 | MS.gene036595:intron | 45.0% | |
| GTTCTCCCAAACATTCTCAC+AGG | + | chr8.3:8749641-8749660 | None:intergenic | 45.0% | |
| TTCAACTGACCTGTCTGGAA+GGG | + | chr8.3:8749549-8749568 | None:intergenic | 45.0% | |
| TTGACACCGACATCAAAACG+AGG | + | chr8.3:8749496-8749515 | None:intergenic | 45.0% | |
| TTTCAACTGACCTGTCTGGA+AGG | + | chr8.3:8749550-8749569 | None:intergenic | 45.0% | |
| !! | AATGTTGGTGGTAAGGTCCT+TGG | - | chr8.3:8750644-8750663 | MS.gene036595:CDS | 45.0% |
| !!! | TCGTTTTGATGTCGGTGTCA+AGG | - | chr8.3:8749495-8749514 | MS.gene036595:intron | 45.0% |
| !! | TTATAATTATAAAAATGTAA+GGG | + | chr8.3:8750552-8750571 | None:intergenic | 5.0% |
| !! | TTTATAATTATAAAAATGTA+AGG | + | chr8.3:8750553-8750572 | None:intergenic | 5.0% |
| TAGACTTCTCCCTTCCAGAC+AGG | - | chr8.3:8749537-8749556 | MS.gene036595:intron | 50.0% | |
| !! | GTCGGTGTCAAGGAGATTGA+AGG | - | chr8.3:8749505-8749524 | MS.gene036595:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 8749109 | 8750679 | 8749109 | ID=MS.gene036595 |
| chr8.3 | mRNA | 8749109 | 8750679 | 8749109 | ID=MS.gene036595.t1;Parent=MS.gene036595 |
| chr8.3 | exon | 8750547 | 8750679 | 8750547 | ID=MS.gene036595.t1.exon1;Parent=MS.gene036595.t1 |
| chr8.3 | CDS | 8750547 | 8750679 | 8750547 | ID=cds.MS.gene036595.t1;Parent=MS.gene036595.t1 |
| chr8.3 | exon | 8750230 | 8750390 | 8750230 | ID=MS.gene036595.t1.exon2;Parent=MS.gene036595.t1 |
| chr8.3 | CDS | 8750230 | 8750390 | 8750230 | ID=cds.MS.gene036595.t1;Parent=MS.gene036595.t1 |
| chr8.3 | exon | 8749109 | 8749207 | 8749109 | ID=MS.gene036595.t1.exon3;Parent=MS.gene036595.t1 |
| chr8.3 | CDS | 8749109 | 8749207 | 8749109 | ID=cds.MS.gene036595.t1;Parent=MS.gene036595.t1 |
| Gene Sequence |
| Protein sequence |
