AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58719


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58719.t1 MTR_4g117470 100.000 130 0 0 1 130 1 130 1.05e-93 266
MS.gene58719.t1 MTR_4g094598 99.231 130 1 0 1 130 1 130 2.82e-93 265
MS.gene58719.t1 MTR_1g105415 99.231 130 1 0 1 130 1 130 2.82e-93 265
MS.gene58719.t1 MTR_8g101910 52.000 125 60 0 6 130 5 129 1.82e-45 144
MS.gene58719.t1 MTR_8g101910 49.474 95 48 0 6 100 5 99 1.72e-28 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58719.t1 AT5G59850 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene58719.t1 AT1G07770 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene58719.t1 AT1G07770 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene58719.t1 AT1G07770 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene58719.t1 AT3G46040 97.692 130 3 0 1 130 1 130 4.03e-92 262
MS.gene58719.t1 AT2G39590 91.538 130 11 0 1 130 1 130 4.30e-87 249
MS.gene58719.t1 AT4G29430 54.400 125 57 0 6 130 5 129 2.98e-47 149
MS.gene58719.t1 AT2G19720 53.600 125 58 0 6 130 5 129 3.11e-45 144

Find 30 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCACTTCTGCTGGTATTA+TGG 0.215449 8.1:-9860574 MS.gene58719:CDS
TTATGCTTTATAGGTTATAT+TGG 0.301097 8.1:-9861786 MS.gene58719:intron
TATGATTAGGCCTTCCTCTA+AGG 0.385448 8.1:-9861970 MS.gene58719:CDS
GAATTTGATGATGACCTTAG+AGG 0.411030 8.1:+9861956 None:intergenic
GAAAGCGTCAAGTTATGATT+AGG 0.452540 8.1:-9861983 MS.gene58719:CDS
TATGTTGATGATCACAGATC+TGG 0.452973 8.1:-9861753 MS.gene58719:CDS
GGTCCTTGGCTTCTTCTACT+AGG 0.468896 8.1:-9860514 None:intergenic
AATGTTGGTGGTAAGGTCCT+TGG 0.482167 8.1:-9860528 MS.gene58719:CDS
AAAATCGTTGTTGAATTGAA+TGG 0.485579 8.1:-9861729 MS.gene58719:CDS
GAGAAAGAATGTTGGTGGTA+AGG 0.495772 8.1:-9860535 MS.gene58719:CDS
ATGGTAGGTTGAACAAATGT+GGG 0.519393 8.1:-9861710 MS.gene58719:CDS
ATCCATAATACCAGCAGAAG+TGG 0.531152 8.1:+9860572 None:intergenic
TAGACTTCTCCCTTCCAGAC+AGG 0.537117 8.1:-9861634 MS.gene58719:intron
ATGTACAATGCTGAGAAAAG+AGG 0.544704 8.1:-9862005 MS.gene58719:CDS
TTCAACTGACCTGTCTGGAA+GGG 0.545440 8.1:+9861625 None:intergenic
TTTCAACTGACCTGTCTGGA+AGG 0.549780 8.1:+9861624 None:intergenic
ATGTTGATGATCACAGATCT+GGG 0.565212 8.1:-9861752 MS.gene58719:CDS
GCTAGGAGAAAGAATGTTGG+TGG 0.567141 8.1:-9860540 MS.gene58719:CDS
TAGCCTAGTAGAAGAAGCCA+AGG 0.575489 8.1:+9860511 None:intergenic
AATGGTAGGTTGAACAAATG+TGG 0.577364 8.1:-9861711 MS.gene58719:CDS
GAGGCTAGGAGAAAGAATGT+TGG 0.584494 8.1:-9860543 MS.gene58719:CDS
TTATGGATCATGAAGAGGCT+AGG 0.585160 8.1:-9860557 MS.gene58719:CDS
TGGTATTATGGATCATGAAG+AGG 0.606467 8.1:-9860562 MS.gene58719:CDS
TTGATGATGACCTTAGAGGA+AGG 0.613228 8.1:+9861960 None:intergenic
GTCGGTGTCAAGGAGATTGA+AGG 0.613514 8.1:-9861666 MS.gene58719:CDS
TCGTTGTTGAATTGAATGGT+AGG 0.614641 8.1:-9861725 MS.gene58719:CDS
CAAAACACTGACTCTCACCA+TGG 0.638914 8.1:+9862046 None:intergenic
GTGTCAAGGAGATTGAAGGT+TGG 0.653545 8.1:-9861662 MS.gene58719:CDS
TGGTAGGTTGAACAAATGTG+GGG 0.708928 8.1:-9861709 MS.gene58719:CDS
TTGACACCGACATCAAAACG+AGG 0.734537 8.1:+9861678 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTAAATAATAACAATTTA+AGG + chr8.1:9861849-9861868 None:intergenic 10.0%
!! CTAAAATTGATCATAAAAAA+CGG + chr8.1:9861725-9861744 None:intergenic 15.0%
!! TAATTATAAAATATGCAGTT+TGG - chr8.1:9861950-9861969 MS.gene58719:CDS 15.0%
!!! AACTTAATTTATCTTTTGAT+AGG - chr8.1:9861135-9861154 MS.gene58719:intron 15.0%
!!! TGAAAACAATACTAATTTTT+GGG - chr8.1:9860953-9860972 MS.gene58719:intron 15.0%
!!! TTGAAAACAATACTAATTTT+TGG - chr8.1:9860952-9860971 MS.gene58719:intron 15.0%
!!! TTGATTTGATATAATTTTGT+GGG - chr8.1:9860698-9860717 MS.gene58719:intron 15.0%
!!! TTTGATTTGATATAATTTTG+TGG - chr8.1:9860697-9860716 MS.gene58719:intron 15.0%
!!! TTTGATTTGATATATTTTGT+GGG - chr8.1:9860750-9860769 MS.gene58719:intron 15.0%
!!! TTTTGATTTGATATATTTTG+TGG - chr8.1:9860749-9860768 MS.gene58719:intron 15.0%
!! AAAACTAATAAATGTTGCAT+TGG + chr8.1:9861246-9861265 None:intergenic 20.0%
!! GGATGAAATTCATTAAATAA+GGG + chr8.1:9861282-9861301 None:intergenic 20.0%
!! TGGATGAAATTCATTAAATA+AGG + chr8.1:9861283-9861302 None:intergenic 20.0%
!! TTATGCTTTATAGGTTATAT+TGG - chr8.1:9860773-9860792 MS.gene58719:intron 20.0%
!! TTGCATGATTCAATTATTTA+GGG - chr8.1:9860673-9860692 MS.gene58719:intron 20.0%
!!! AAAAAACGGAGATTTTTTTT+AGG + chr8.1:9861711-9861730 None:intergenic 20.0%
!!! ATATTTTTTCCTTTCATTGA+AGG - chr8.1:9860650-9860669 MS.gene58719:intron 20.0%
!!! ATTAATTGTGTTGATTTCAA+TGG + chr8.1:9861366-9861385 None:intergenic 20.0%
!!! ATTTAACTTATATGAACGTT+GGG - chr8.1:9861875-9861894 MS.gene58719:intron 20.0%
!!! TATTTAACTTATATGAACGT+TGG - chr8.1:9861874-9861893 MS.gene58719:intron 20.0%
! AAAATCGTTGTTGAATTGAA+TGG - chr8.1:9860830-9860849 MS.gene58719:intron 25.0%
! AAACAACATATACACACAAA+AGG + chr8.1:9861906-9861925 None:intergenic 25.0%
! GTTGCATGATTCAATTATTT+AGG - chr8.1:9860672-9860691 MS.gene58719:intron 25.0%
! TATAGTTTCTTACTCCTATT+TGG - chr8.1:9861656-9861675 MS.gene58719:CDS 25.0%
! TCATGCTTCATATTAAAGAT+TGG - chr8.1:9861620-9861639 MS.gene58719:intron 25.0%
!! TATAATTTTGTGGGTATGTT+TGG - chr8.1:9860707-9860726 MS.gene58719:intron 25.0%
!! TGATTTGGAACAAATTTACT+CGG + chr8.1:9861593-9861612 None:intergenic 25.0%
!!! TCAGCTTATTTTCATAAACA+TGG - chr8.1:9861086-9861105 MS.gene58719:intron 25.0%
!!! TTTGGTGGTTCAATTTTTTA+GGG - chr8.1:9860725-9860744 MS.gene58719:intron 25.0%
AAACGACTAATATTGTGAGA+CGG - chr8.1:9861415-9861434 MS.gene58719:intron 30.0%
AAGCATGAACTATCATGATT+TGG + chr8.1:9861608-9861627 None:intergenic 30.0%
AAGTTCACACAAATATGCAT+TGG + chr8.1:9861520-9861539 None:intergenic 30.0%
ACACCCTTATTAATTGTAGT+TGG + chr8.1:9861320-9861339 None:intergenic 30.0%
ATTTACTCGGCTTAACAAAA+TGG + chr8.1:9861580-9861599 None:intergenic 30.0%
CATAGCTATATGTAAGAACA+TGG + chr8.1:9861481-9861500 None:intergenic 30.0%
CCACCAACTACAATTAATAA+GGG - chr8.1:9861314-9861333 MS.gene58719:intron 30.0%
CTCACAATATTAGTCGTTTA+AGG + chr8.1:9861414-9861433 None:intergenic 30.0%
TATCACTTGTGAGAATGTTT+GGG - chr8.1:9861021-9861040 MS.gene58719:intron 30.0%
TCCACCAACTACAATTAATA+AGG - chr8.1:9861313-9861332 MS.gene58719:intron 30.0%
TTATCACTTGTGAGAATGTT+TGG - chr8.1:9861020-9861039 MS.gene58719:intron 30.0%
TTTGGGAGAACTTATGAAAA+CGG - chr8.1:9861038-9861057 MS.gene58719:intron 30.0%
!! TCAATTTTAGCCTTTACTAG+AGG - chr8.1:9861735-9861754 MS.gene58719:CDS 30.0%
!! TTTTGTGGGTTATGCTTTAT+AGG - chr8.1:9860764-9860783 MS.gene58719:intron 30.0%
!!! GTTTGGTGGTTCAATTTTTT+AGG - chr8.1:9860724-9860743 MS.gene58719:intron 30.0%
ACACAAATATGCATTGGAAG+TGG + chr8.1:9861514-9861533 None:intergenic 35.0%
AGGTTTGTGCACGATTATTA+AGG + chr8.1:9861394-9861413 None:intergenic 35.0%
AGTAAGCTGTTTATCCAAAC+AGG - chr8.1:9861454-9861473 MS.gene58719:intron 35.0%
ATCATGCAACCTTCAATGAA+AGG + chr8.1:9860662-9860681 None:intergenic 35.0%
ATGTACAATGCTGAGAAAAG+AGG - chr8.1:9860554-9860573 MS.gene58719:CDS 35.0%
CACATAAAAGCCTCTAGTAA+AGG + chr8.1:9861748-9861767 None:intergenic 35.0%
CCCTTATTAATTGTAGTTGG+TGG + chr8.1:9861317-9861336 None:intergenic 35.0%
CTCATCGTTATGCAAAAACA+CGG - chr8.1:9860626-9860645 MS.gene58719:intron 35.0%
TCGTTGTTGAATTGAATGGT+AGG - chr8.1:9860834-9860853 MS.gene58719:intron 35.0%
TGGTATTATGGATCATGAAG+AGG - chr8.1:9861997-9862016 MS.gene58719:CDS 35.0%
TTGTTCCACAAAGACCAAAT+AGG + chr8.1:9861673-9861692 None:intergenic 35.0%
! AATGGTAGGTTGAACAAATG+TGG - chr8.1:9860848-9860867 MS.gene58719:intron 35.0%
! AATTTTGTGGGTATGTTTGG+TGG - chr8.1:9860710-9860729 MS.gene58719:intron 35.0%
! ATGGTAGGTTGAACAAATGT+GGG - chr8.1:9860849-9860868 MS.gene58719:intron 35.0%
! ATTTGTTGCTTGGATGATCT+AGG - chr8.1:9861797-9861816 MS.gene58719:intron 35.0%
! TTACTAGAGGCTTTTATGTG+TGG - chr8.1:9861748-9861767 MS.gene58719:CDS 35.0%
! TTACTCCTATTTGGTCTTTG+TGG - chr8.1:9861665-9861684 MS.gene58719:CDS 35.0%
!! ATGTTGATGATCACAGATCT+GGG - chr8.1:9860807-9860826 MS.gene58719:intron 35.0%
!! ATTAGTCCTCGTTTTGATGT+CGG - chr8.1:9860875-9860894 MS.gene58719:intron 35.0%
!! GAAAGCGTCAAGTTATGATT+AGG - chr8.1:9860576-9860595 MS.gene58719:CDS 35.0%
!! GAATTTGATGATGACCTTAG+AGG + chr8.1:9860606-9860625 None:intergenic 35.0%
!! TATGTTGATGATCACAGATC+TGG - chr8.1:9860806-9860825 MS.gene58719:intron 35.0%
!! TCTGTCAACAATTTGTTGCT+TGG - chr8.1:9861787-9861806 MS.gene58719:intron 35.0%
ATCCATAATACCAGCAGAAG+TGG + chr8.1:9861990-9862009 None:intergenic 40.0%
GACTAATATTGTGAGACGGA+GGG - chr8.1:9861419-9861438 MS.gene58719:intron 40.0%
TATGATTAGGCCTTCCTCTA+AGG - chr8.1:9860589-9860608 MS.gene58719:CDS 40.0%
TGTAAGAACATGGTCCTGTT+TGG + chr8.1:9861471-9861490 None:intergenic 40.0%
TTATGGATCATGAAGAGGCT+AGG - chr8.1:9862002-9862021 MS.gene58719:CDS 40.0%
! CTTCAGTGTTATCGATTGCA+TGG - chr8.1:9861551-9861570 MS.gene58719:intron 40.0%
! TGGTAGGTTGAACAAATGTG+GGG - chr8.1:9860850-9860869 MS.gene58719:intron 40.0%
! TTCTTTTGCATAGTGTCTGC+TGG - chr8.1:9860980-9860999 MS.gene58719:intron 40.0%
! TTGTTTTCAACTGACCTGTC+TGG + chr8.1:9860942-9860961 None:intergenic 40.0%
!! AGTTGGTGGAGTAGTTAAGT+TGG + chr8.1:9861303-9861322 None:intergenic 40.0%
!! CGTGTTTTTGCATAACGATG+AGG + chr8.1:9860628-9860647 None:intergenic 40.0%
!! GAGAAAGAATGTTGGTGGTA+AGG - chr8.1:9862024-9862043 MS.gene58719:CDS 40.0%
!! TTGATGATGACCTTAGAGGA+AGG + chr8.1:9860602-9860621 None:intergenic 40.0%
!!! ATTGTTTTGACCACTTCTGC+TGG - chr8.1:9861977-9861996 MS.gene58719:CDS 40.0%
CGACTAATATTGTGAGACGG+AGG - chr8.1:9861418-9861437 MS.gene58719:intron 45.0%
GACCACTTCTGCTGGTATTA+TGG - chr8.1:9861985-9862004 MS.gene58719:CDS 45.0%
GAGGCTAGGAGAAAGAATGT+TGG - chr8.1:9862016-9862035 MS.gene58719:CDS 45.0%
GCTAGGAGAAAGAATGTTGG+TGG - chr8.1:9862019-9862038 MS.gene58719:CDS 45.0%
GTGTCAAGGAGATTGAAGGT+TGG - chr8.1:9860897-9860916 MS.gene58719:intron 45.0%
TTCAACTGACCTGTCTGGAA+GGG + chr8.1:9860937-9860956 None:intergenic 45.0%
TTGACACCGACATCAAAACG+AGG + chr8.1:9860884-9860903 None:intergenic 45.0%
TTTCAACTGACCTGTCTGGA+AGG + chr8.1:9860938-9860957 None:intergenic 45.0%
!! AATGTTGGTGGTAAGGTCCT+TGG - chr8.1:9862031-9862050 MS.gene58719:CDS 45.0%
!!! TCGTTTTGATGTCGGTGTCA+AGG - chr8.1:9860883-9860902 MS.gene58719:intron 45.0%
!! TTATAATTATAAAAATGTAA+GGG + chr8.1:9861939-9861958 None:intergenic 5.0%
!! TTTATAATTATAAAAATGTA+AGG + chr8.1:9861940-9861959 None:intergenic 5.0%
TAGACTTCTCCCTTCCAGAC+AGG - chr8.1:9860925-9860944 MS.gene58719:intron 50.0%
!! GTCGGTGTCAAGGAGATTGA+AGG - chr8.1:9860893-9860912 MS.gene58719:intron 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 9860515 9862066 9860515 ID=MS.gene58719
chr8.1 mRNA 9860515 9862066 9860515 ID=MS.gene58719.t1;Parent=MS.gene58719
chr8.1 exon 9861934 9862066 9861934 ID=MS.gene58719.t1.exon1;Parent=MS.gene58719.t1
chr8.1 CDS 9861934 9862066 9861934 ID=cds.MS.gene58719.t1;Parent=MS.gene58719.t1
chr8.1 exon 9861635 9861795 9861635 ID=MS.gene58719.t1.exon2;Parent=MS.gene58719.t1
chr8.1 CDS 9861635 9861795 9861635 ID=cds.MS.gene58719.t1;Parent=MS.gene58719.t1
chr8.1 exon 9860515 9860613 9860515 ID=MS.gene58719.t1.exon3;Parent=MS.gene58719.t1
chr8.1 CDS 9860515 9860613 9860515 ID=cds.MS.gene58719.t1;Parent=MS.gene58719.t1
Gene Sequence

>MS.gene58719

ATGGTGAGAGTCAGTGTTTTGAACGATGCTCTTAAGAGTATGTACAATGCTGAGAAAAGAGGAAAGCGTCAAGTTATGATTAGGCCTTCCTCTAAGGTCATCATCAAATTCCTCATCGTTATGCAAAAACACGGTATATTTTTTCCTTTCATTGAAGGTTGCATGATTCAATTATTTAGGGTTTTGATTTGATATAATTTTGTGGGTATGTTTGGTGGTTCAATTTTTTAGGGTTTTTGATTTGATATATTTTGTGGGTTATGCTTTATAGGTTATATTGGAGAGTTTGAGTATGTTGATGATCACAGATCTGGGAAAATCGTTGTTGAATTGAATGGTAGGTTGAACAAATGTGGGGTTATTAGTCCTCGTTTTGATGTCGGTGTCAAGGAGATTGAAGGTTGGACTGCTAGACTTCTCCCTTCCAGACAGGTCAGTTGAAAACAATACTAATTTTTGGGAAACTTCTTTTGCATAGTGTCTGCTGGAATTGACTTATTTGAGCTTATCACTTGTGAGAATGTTTGGGAGAACTTATGAAAACGGCTTATGACATGTTCATAAGCTATTTTCAGCTTATTTTCATAAACATGGCTTATAGCTTATAAGATAACAATCTAAACTTAATTTATCTTTTGATAGGTGTTTATGTTATACTCTCTCTGTCTCACAATTATTGTCTCTTTAGCATTTTCTCTATTTCTCAAAATAATTGTCACTTTACAATACCAATGCAACATTTATTAGTTTTTTCTACTATTATACCCTTATTTAATGAATTTCATCCAACTTAACTACTCCACCAACTACAATTAATAAGGGTGTTTTAGTAAATGCTACTAATTTTACCATTGAAATCAACACAATTAATCATTTCCTTAATAATCGTGCACAAACCTTAAACGACTAATATTGTGAGACGGAGGGAGTAAGCTGTAAAGTAAGCTGTTTATCCAAACAGGACCATGTTCTTACATATAGCTATGCTAGCATGTTCCACTTCCAATGCATATTTGTGTGAACTTTGAAGAGCTTTCTTCAGTGTTATCGATTGCATGGTTACCATTTTGTTAAGCCGAGTAAATTTGTTCCAAATCATGATAGTTCATGCTTCATATTAAAGATTGGCTTGTTTCTTTTGTATAGTTTCTTACTCCTATTTGGTCTTTGTGGAACAAAAAACACAGTTTAGTCCTAAAAAAAATCTCCGTTTTTTATGATCAATTTTAGCCTTTACTAGAGGCTTTTATGTGTGGAATTAGTAGTTTGAGCTCTGTCAACAATTTGTTGCTTGGATGATCTAGGTTGATGATTTGTTCTTGTGCTGCTGTCCTTAAATTGTTATTATTTAAGATCAAATATTTAACTTATATGAACGTTGGGTCTTTCCTTTTGTGTGTATATGTTGTTTCTTCTGTTTTCCCTTACATTTTTATAATTATAAAATATGCAGTTTGGATACATTGTTTTGACCACTTCTGCTGGTATTATGGATCATGAAGAGGCTAGGAGAAAGAATGTTGGTGGTAAGGTCCTTGGCTTCTTCTACTAG

Protein sequence

>MS.gene58719.t1

MVRVSVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLIVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEGWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY