Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041458.t1 | NP_001275136.1 | 100 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 8.20E-68 | 266.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041458.t1 | P42798 | 99.2 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 2.6e-70 | 265.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041458.t1 | A0A1U7YEG5 | 100.0 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 5.9e-68 | 266.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene36468 | MS.gene041458 | PPI |
| MS.gene041458 | MS.gene023130 | PPI |
| MS.gene028323 | MS.gene041458 | PPI |
| MS.gene041458 | MS.gene06172 | PPI |
| MS.gene041458 | MS.gene58227 | PPI |
| MS.gene55887 | MS.gene041458 | PPI |
| MS.gene35135 | MS.gene041458 | PPI |
| MS.gene041458 | MS.gene20156 | PPI |
| MS.gene041458 | MS.gene58719 | PPI |
| MS.gene041458 | MS.gene036595 | PPI |
| MS.gene041458 | MS.gene049026 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041458.t1 | MTR_4g117470 | 100.000 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 1.05e-93 | 266 |
| MS.gene041458.t1 | MTR_4g094598 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 2.82e-93 | 265 |
| MS.gene041458.t1 | MTR_1g105415 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 2.82e-93 | 265 |
| MS.gene041458.t1 | MTR_8g101910 | 52.000 | 125 | 60 | 0 | 6 | 130 | 5 | 129 | 1.82e-45 | 144 |
| MS.gene041458.t1 | MTR_8g101910 | 49.474 | 95 | 48 | 0 | 6 | 100 | 5 | 99 | 1.72e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041458.t1 | AT5G59850 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.76e-93 | 265 |
| MS.gene041458.t1 | AT1G07770 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.76e-93 | 265 |
| MS.gene041458.t1 | AT1G07770 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.76e-93 | 265 |
| MS.gene041458.t1 | AT1G07770 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.76e-93 | 265 |
| MS.gene041458.t1 | AT3G46040 | 97.692 | 130 | 3 | 0 | 1 | 130 | 1 | 130 | 4.03e-92 | 262 |
| MS.gene041458.t1 | AT2G39590 | 91.538 | 130 | 11 | 0 | 1 | 130 | 1 | 130 | 4.30e-87 | 249 |
| MS.gene041458.t1 | AT4G29430 | 54.400 | 125 | 57 | 0 | 6 | 130 | 5 | 129 | 2.98e-47 | 149 |
| MS.gene041458.t1 | AT2G19720 | 53.600 | 125 | 58 | 0 | 6 | 130 | 5 | 129 | 3.11e-45 | 144 |
Find 30 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACCACTTCTGCTGGTATTA+TGG | 0.215449 | 8.2:-11937863 | MS.gene041458:CDS |
| TTATGCTTTATAGGTTATAT+TGG | 0.301097 | 8.2:-11938813 | MS.gene041458:intron |
| TATGATTAGGCCTTCCTCTA+AGG | 0.385448 | 8.2:-11939011 | MS.gene041458:CDS |
| GAATTTGATGATGACCTTAG+AGG | 0.411030 | 8.2:+11938997 | None:intergenic |
| GAAAGCGTCAAGTTATGATT+AGG | 0.452540 | 8.2:-11939024 | MS.gene041458:CDS |
| TATGTTGATGATCACAGATC+TGG | 0.452973 | 8.2:-11938780 | MS.gene041458:CDS |
| GGTCCTTGGCTTCTTCTACT+AGG | 0.468896 | 8.2:-11937803 | None:intergenic |
| AATGTTGGTGGTAAGGTCCT+TGG | 0.482167 | 8.2:-11937817 | MS.gene041458:CDS |
| AAAATCGTTGTTGAATTGAA+TGG | 0.485579 | 8.2:-11938756 | MS.gene041458:CDS |
| GAGAAAGAATGTTGGTGGTA+AGG | 0.495772 | 8.2:-11937824 | MS.gene041458:CDS |
| ATGGTAGGTTGAACAAATGT+GGG | 0.519393 | 8.2:-11938737 | MS.gene041458:CDS |
| ATCCATAATACCAGCAGAAG+TGG | 0.531152 | 8.2:+11937861 | None:intergenic |
| TAGACTTCTCCCTTCCAGAC+AGG | 0.537117 | 8.2:-11938661 | MS.gene041458:intron |
| ATGTACAATGCTGAGAAAAG+AGG | 0.544704 | 8.2:-11939046 | MS.gene041458:CDS |
| TTCAACTGACCTGTCTGGAA+GGG | 0.545440 | 8.2:+11938652 | None:intergenic |
| TTTCAACTGACCTGTCTGGA+AGG | 0.549780 | 8.2:+11938651 | None:intergenic |
| ATGTTGATGATCACAGATCT+GGG | 0.565212 | 8.2:-11938779 | MS.gene041458:CDS |
| GCTAGGAGAAAGAATGTTGG+TGG | 0.567141 | 8.2:-11937829 | MS.gene041458:CDS |
| TAGCCTAGTAGAAGAAGCCA+AGG | 0.575489 | 8.2:+11937800 | None:intergenic |
| AATGGTAGGTTGAACAAATG+TGG | 0.577364 | 8.2:-11938738 | MS.gene041458:CDS |
| GAGGCTAGGAGAAAGAATGT+TGG | 0.584494 | 8.2:-11937832 | MS.gene041458:CDS |
| TTATGGATCATGAAGAGGCT+AGG | 0.585160 | 8.2:-11937846 | MS.gene041458:CDS |
| TGGTATTATGGATCATGAAG+AGG | 0.606467 | 8.2:-11937851 | MS.gene041458:CDS |
| TTGATGATGACCTTAGAGGA+AGG | 0.613228 | 8.2:+11939001 | None:intergenic |
| GTCGGTGTCAAGGAGATTGA+AGG | 0.613514 | 8.2:-11938693 | MS.gene041458:CDS |
| TCGTTGTTGAATTGAATGGT+AGG | 0.614641 | 8.2:-11938752 | MS.gene041458:CDS |
| CAACACACTGACTCTCACCA+TGG | 0.647471 | 8.2:+11939087 | None:intergenic |
| GTGTCAAGGAGATTGAAGGT+TGG | 0.653545 | 8.2:-11938689 | MS.gene041458:CDS |
| TGGTAGGTTGAACAAATGTG+GGG | 0.708928 | 8.2:-11938736 | MS.gene041458:CDS |
| TTGACACCGACATCAAAACG+AGG | 0.734537 | 8.2:+11938705 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAATTATAAAAATATAA+GGG | + | chr8.2:11938980-11938999 | None:intergenic | 0.0% |
| !! | TTTATAATTATAAAAATATA+AGG | + | chr8.2:11938981-11939000 | None:intergenic | 0.0% |
| !! | CTAAAATTGATCATAAAAAA+CGG | + | chr8.2:11938766-11938785 | None:intergenic | 15.0% |
| !! | TAATTATAAAATTTGCAGTT+TGG | - | chr8.2:11938991-11939010 | MS.gene041458:CDS | 15.0% |
| !! | TCTTAAATAATAACAACTTA+AGG | + | chr8.2:11938890-11938909 | None:intergenic | 15.0% |
| !!! | AATCTTTTTTTTTTCATTGA+AGG | - | chr8.2:11937953-11937972 | MS.gene041458:intron | 15.0% |
| !!! | TGAAAACAATACTAATTTTT+GGG | - | chr8.2:11938256-11938275 | MS.gene041458:intron | 15.0% |
| !!! | TTGAAAACAATACTAATTTT+TGG | - | chr8.2:11938255-11938274 | MS.gene041458:intron | 15.0% |
| !!! | TTGATTTGATATAATTTTGT+GGG | - | chr8.2:11938001-11938020 | MS.gene041458:intron | 15.0% |
| !!! | TTTGATTTGATATAATTTTG+TGG | - | chr8.2:11938000-11938019 | MS.gene041458:intron | 15.0% |
| !!! | TTTGATTTGATATATTTTGT+GGG | - | chr8.2:11938053-11938072 | MS.gene041458:intron | 15.0% |
| !!! | TTTTGATTTGATATATTTTG+TGG | - | chr8.2:11938052-11938071 | MS.gene041458:intron | 15.0% |
| !! | TTATGCTTTATAGGTTATAT+TGG | - | chr8.2:11938076-11938095 | MS.gene041458:intron | 20.0% |
| !! | TTGCATGATTCAATTATTTA+GGG | - | chr8.2:11937976-11937995 | MS.gene041458:intron | 20.0% |
| !!! | TAAAAAACGGAGATTTTTTT+AGG | + | chr8.2:11938753-11938772 | None:intergenic | 20.0% |
| ! | AAAATCGTTGTTGAATTGAA+TGG | - | chr8.2:11938133-11938152 | MS.gene041458:intron | 25.0% |
| ! | AAACAACATATACACACAAA+AGG | + | chr8.2:11938947-11938966 | None:intergenic | 25.0% |
| ! | ATTGAACTTATATGAACGTT+GGG | - | chr8.2:11938916-11938935 | MS.gene041458:intron | 25.0% |
| ! | GTTGCATGATTCAATTATTT+AGG | - | chr8.2:11937975-11937994 | MS.gene041458:intron | 25.0% |
| ! | TATAGTTTCTTACTCCTATT+TGG | - | chr8.2:11938697-11938716 | MS.gene041458:CDS | 25.0% |
| ! | TATTGAACTTATATGAACGT+TGG | - | chr8.2:11938915-11938934 | MS.gene041458:intron | 25.0% |
| ! | TCATGCTTCATATTAAAGAT+TGG | - | chr8.2:11938661-11938680 | MS.gene041458:intron | 25.0% |
| !! | TATAATTTTGTGGGTATGTT+AGG | - | chr8.2:11938010-11938029 | MS.gene041458:intron | 25.0% |
| !! | TGATTTGGAACAAATTTACT+CGG | + | chr8.2:11938634-11938653 | None:intergenic | 25.0% |
| !!! | AGATTTTTTTAGGACTAAAC+TGG | + | chr8.2:11938743-11938762 | None:intergenic | 25.0% |
| !!! | TCAGCTTATTTTCATAAACA+TGG | - | chr8.2:11938377-11938396 | MS.gene041458:intron | 25.0% |
| !!! | TTAGGTGGTTCAATTTTTTA+GGG | - | chr8.2:11938028-11938047 | MS.gene041458:intron | 25.0% |
| AAGTTCACACAAATATGCAT+TGG | + | chr8.2:11938561-11938580 | None:intergenic | 30.0% | |
| TATCACTTGTGAGAATGTTT+GGG | - | chr8.2:11938324-11938343 | MS.gene041458:intron | 30.0% | |
| TTATCACTTGTGAGAATGTT+TGG | - | chr8.2:11938323-11938342 | MS.gene041458:intron | 30.0% | |
| ! | CTTCAGTATTATCGATTGTA+TGG | - | chr8.2:11938592-11938611 | MS.gene041458:intron | 30.0% |
| !! | TTTTGTGGGTTATGCTTTAT+AGG | - | chr8.2:11938067-11938086 | MS.gene041458:intron | 30.0% |
| !!! | GTTAGGTGGTTCAATTTTTT+AGG | - | chr8.2:11938027-11938046 | MS.gene041458:intron | 30.0% |
| AAGCATGAACTATCGTGATT+TGG | + | chr8.2:11938649-11938668 | None:intergenic | 35.0% | |
| ACACAAATATGCATTGGAAG+TGG | + | chr8.2:11938555-11938574 | None:intergenic | 35.0% | |
| AGAATGTTTGGGAGAACTTA+TGG | - | chr8.2:11938335-11938354 | MS.gene041458:intron | 35.0% | |
| AGTAAGCTGTTTATCCAAAC+AGG | - | chr8.2:11938495-11938514 | MS.gene041458:intron | 35.0% | |
| ATGTACAATGCTGAGAAAAG+AGG | - | chr8.2:11937843-11937862 | MS.gene041458:CDS | 35.0% | |
| CTCATCGTTATGCAAAAACA+CGG | - | chr8.2:11937915-11937934 | MS.gene041458:intron | 35.0% | |
| GCACAAGTGTTTATCATGAT+AGG | - | chr8.2:11938451-11938470 | MS.gene041458:intron | 35.0% | |
| TCGTTGTTGAATTGAATGGT+AGG | - | chr8.2:11938137-11938156 | MS.gene041458:intron | 35.0% | |
| TGGTATTATGGATCATGAAG+AGG | - | chr8.2:11939038-11939057 | MS.gene041458:CDS | 35.0% | |
| TTGTTCCACAAAGACCAAAT+AGG | + | chr8.2:11938714-11938733 | None:intergenic | 35.0% | |
| ! | AATGGTAGGTTGAACAAATG+TGG | - | chr8.2:11938151-11938170 | MS.gene041458:intron | 35.0% |
| ! | AATTTTGTGGGTATGTTAGG+TGG | - | chr8.2:11938013-11938032 | MS.gene041458:intron | 35.0% |
| ! | ATGGTAGGTTGAACAAATGT+GGG | - | chr8.2:11938152-11938171 | MS.gene041458:intron | 35.0% |
| ! | ATTTACTCGGCTTGACAAAA+TGG | + | chr8.2:11938621-11938640 | None:intergenic | 35.0% |
| ! | ATTTGTTGCTTGGATGATCT+AGG | - | chr8.2:11938838-11938857 | MS.gene041458:intron | 35.0% |
| ! | TTACTCCTATTTGGTCTTTG+TGG | - | chr8.2:11938706-11938725 | MS.gene041458:CDS | 35.0% |
| !! | ATGTTGATGATCACAGATCT+GGG | - | chr8.2:11938110-11938129 | MS.gene041458:intron | 35.0% |
| !! | ATTAGTCCTCGTTTTGATGT+CGG | - | chr8.2:11938178-11938197 | MS.gene041458:intron | 35.0% |
| !! | GAAAGCGTCAAGTTATGATT+AGG | - | chr8.2:11937865-11937884 | MS.gene041458:CDS | 35.0% |
| !! | GAATTTGATGATGACCTTAG+AGG | + | chr8.2:11937895-11937914 | None:intergenic | 35.0% |
| !! | TATGTTGATGATCACAGATC+TGG | - | chr8.2:11938109-11938128 | MS.gene041458:intron | 35.0% |
| !! | TCAATTTTAGCCTCTACTAG+AGG | - | chr8.2:11938776-11938795 | MS.gene041458:CDS | 35.0% |
| !! | TCTGTCAACAATTTGTTGCT+TGG | - | chr8.2:11938828-11938847 | MS.gene041458:intron | 35.0% |
| ATCCATAATACCAGCAGAAG+TGG | + | chr8.2:11939031-11939050 | None:intergenic | 40.0% | |
| CACATAAAAGCCTCTAGTAG+AGG | + | chr8.2:11938789-11938808 | None:intergenic | 40.0% | |
| CATAGCCATACGTAAGAACA+TGG | + | chr8.2:11938522-11938541 | None:intergenic | 40.0% | |
| TATGATTAGGCCTTCCTCTA+AGG | - | chr8.2:11937878-11937897 | MS.gene041458:CDS | 40.0% | |
| TTATGGATCATGAAGAGGCT+AGG | - | chr8.2:11939043-11939062 | MS.gene041458:CDS | 40.0% | |
| ! | CTACTAGAGGCTTTTATGTG+TGG | - | chr8.2:11938789-11938808 | MS.gene041458:CDS | 40.0% |
| ! | TGGTAGGTTGAACAAATGTG+GGG | - | chr8.2:11938153-11938172 | MS.gene041458:intron | 40.0% |
| ! | TTCTTTTGCATAGTGTCTGC+TGG | - | chr8.2:11938283-11938302 | MS.gene041458:intron | 40.0% |
| ! | TTGTTTTCAACTGACCTGTC+TGG | + | chr8.2:11938245-11938264 | None:intergenic | 40.0% |
| !! | CGTGTTTTTGCATAACGATG+AGG | + | chr8.2:11937917-11937936 | None:intergenic | 40.0% |
| !! | GAGAAAGAATGTTGGTGGTA+AGG | - | chr8.2:11939065-11939084 | MS.gene041458:CDS | 40.0% |
| !! | TTGATGATGACCTTAGAGGA+AGG | + | chr8.2:11937891-11937910 | None:intergenic | 40.0% |
| !!! | ATTGTTTTGACCACTTCTGC+TGG | - | chr8.2:11939018-11939037 | MS.gene041458:CDS | 40.0% |
| CAGGACCATGTTCTTACGTA+TGG | - | chr8.2:11938514-11938533 | MS.gene041458:intron | 45.0% | |
| CGTAAGAACATGGTCCTGTT+TGG | + | chr8.2:11938512-11938531 | None:intergenic | 45.0% | |
| GACCACTTCTGCTGGTATTA+TGG | - | chr8.2:11939026-11939045 | MS.gene041458:CDS | 45.0% | |
| GAGGCTAGGAGAAAGAATGT+TGG | - | chr8.2:11939057-11939076 | MS.gene041458:CDS | 45.0% | |
| GCTAGGAGAAAGAATGTTGG+TGG | - | chr8.2:11939060-11939079 | MS.gene041458:CDS | 45.0% | |
| GTGTCAAGGAGATTGAAGGT+TGG | - | chr8.2:11938200-11938219 | MS.gene041458:intron | 45.0% | |
| TTCAACTGACCTGTCTGGAA+GGG | + | chr8.2:11938240-11938259 | None:intergenic | 45.0% | |
| TTGACACCGACATCAAAACG+AGG | + | chr8.2:11938187-11938206 | None:intergenic | 45.0% | |
| TTTCAACTGACCTGTCTGGA+AGG | + | chr8.2:11938241-11938260 | None:intergenic | 45.0% | |
| !! | AATGTTGGTGGTAAGGTCCT+TGG | - | chr8.2:11939072-11939091 | MS.gene041458:CDS | 45.0% |
| !!! | TCGTTTTGATGTCGGTGTCA+AGG | - | chr8.2:11938186-11938205 | MS.gene041458:intron | 45.0% |
| TAGACTTCTCCCTTCCAGAC+AGG | - | chr8.2:11938228-11938247 | MS.gene041458:intron | 50.0% | |
| !! | GTCGGTGTCAAGGAGATTGA+AGG | - | chr8.2:11938196-11938215 | MS.gene041458:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 11937804 | 11939107 | 11937804 | ID=MS.gene041458 |
| chr8.2 | mRNA | 11937804 | 11939107 | 11937804 | ID=MS.gene041458.t1;Parent=MS.gene041458 |
| chr8.2 | exon | 11938975 | 11939107 | 11938975 | ID=MS.gene041458.t1.exon1;Parent=MS.gene041458.t1 |
| chr8.2 | CDS | 11938975 | 11939107 | 11938975 | ID=cds.MS.gene041458.t1;Parent=MS.gene041458.t1 |
| chr8.2 | exon | 11938662 | 11938822 | 11938662 | ID=MS.gene041458.t1.exon2;Parent=MS.gene041458.t1 |
| chr8.2 | CDS | 11938662 | 11938822 | 11938662 | ID=cds.MS.gene041458.t1;Parent=MS.gene041458.t1 |
| chr8.2 | exon | 11937804 | 11937902 | 11937804 | ID=MS.gene041458.t1.exon3;Parent=MS.gene041458.t1 |
| chr8.2 | CDS | 11937804 | 11937902 | 11937804 | ID=cds.MS.gene041458.t1;Parent=MS.gene041458.t1 |
| Gene Sequence |
| Protein sequence |
