AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55887


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55887.t1 MTR_8g101910 98.450 129 2 0 1 129 1 129 4.67e-94 267
MS.gene55887.t1 MTR_8g101910 96.970 99 3 0 1 99 1 99 3.41e-68 201
MS.gene55887.t1 MTR_4g094598 52.000 125 60 0 5 129 6 130 7.77e-46 145
MS.gene55887.t1 MTR_1g105415 52.000 125 60 0 5 129 6 130 7.77e-46 145
MS.gene55887.t1 MTR_4g117470 52.000 125 60 0 5 129 6 130 1.99e-45 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55887.t1 AT4G29430 81.395 129 24 0 1 129 1 129 5.77e-79 229
MS.gene55887.t1 AT2G19720 79.845 129 26 0 1 129 1 129 1.60e-77 225
MS.gene55887.t1 AT2G39590 52.000 125 60 0 5 129 6 130 3.50e-46 146
MS.gene55887.t1 AT5G59850 52.000 125 60 0 5 129 6 130 1.97e-45 144
MS.gene55887.t1 AT1G07770 52.000 125 60 0 5 129 6 130 1.97e-45 144
MS.gene55887.t1 AT1G07770 52.000 125 60 0 5 129 6 130 1.97e-45 144
MS.gene55887.t1 AT1G07770 52.000 125 60 0 5 129 6 130 1.97e-45 144
MS.gene55887.t1 AT3G46040 50.400 125 62 0 5 129 6 130 2.76e-44 141

Find 33 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTGATCCTCCCCACTCTA+TGG 0.263185 4.3:-3490043 None:intergenic
GAAGAGATAACATTGGAAAT+AGG 0.265767 4.3:-3489267 None:intergenic
TACAACTCCTGATGGTGTTT+TGG 0.307095 4.3:+3490364 MS.gene55887:CDS
AATGTAGGTGGTCAGGTTCT+TGG 0.331557 4.3:+3490410 MS.gene55887:CDS
AGGATATATCAAGGACTTTC+AGG 0.389863 4.3:+3490010 MS.gene55887:intron
CCTTTGCCTTGAGGTCTTGT+CGG 0.398017 4.3:-3490109 None:intergenic
ATCATGAAGAGGCCGCAAAA+AGG 0.404793 4.3:+3490387 MS.gene55887:CDS
TGAGGTCTTGTCGGTACGTA+AGG 0.430606 4.3:-3490100 None:intergenic
TGTGATCCTCCCCACTCTAT+GGG 0.457890 4.3:-3490042 None:intergenic
GAACGATGCGTTGAGGAGTA+TGG 0.475978 4.3:+3489203 MS.gene55887:CDS
GAACACTCTTCCAACACATC+AGG 0.488180 4.3:+3490151 MS.gene55887:CDS
TCATGATCCAAAACACCATC+AGG 0.488311 4.3:-3490371 None:intergenic
AAGAAAAGAAGAGATAACAT+TGG 0.493444 4.3:-3489274 None:intergenic
GAGAAAGAGAAATGGGGAGA+AGG 0.505773 4.3:+3489175 None:intergenic
CATTAACCAACCTGATGTGT+TGG 0.507241 4.3:-3490161 None:intergenic
ATTCAATATCCTTTGCCTTG+AGG 0.544391 4.3:-3490118 None:intergenic
GAAGAGAGGCAAAGCTATGG+TGG 0.562394 4.3:+3489236 MS.gene55887:CDS
GTGTATGATCCCCATAGAGT+GGG 0.576109 4.3:+3490032 MS.gene55887:CDS
CATGTTGGCAGGATATATCA+AGG 0.581279 4.3:+3490001 MS.gene55887:intron
GAGGTCTTGTCGGTACGTAA+GGG 0.585892 4.3:-3490099 None:intergenic
TGAGAAGAGAGGCAAAGCTA+TGG 0.613395 4.3:+3489233 MS.gene55887:CDS
GGTGTATGATCCCCATAGAG+TGG 0.614660 4.3:+3490031 MS.gene55887:CDS
AGGTAGAATTAACGATTGCA+AGG 0.617470 4.3:+3490076 MS.gene55887:CDS
TACGTACCGACAAGACCTCA+AGG 0.647236 4.3:+3490103 MS.gene55887:CDS
CCGACAAGACCTCAAGGCAA+AGG 0.651183 4.3:+3490109 MS.gene55887:CDS
GGATACTGAACGATGCGTTG+AGG 0.657571 4.3:+3489196 MS.gene55887:CDS
AGGATCACAGTTGAACTACA+AGG 0.660202 4.3:+3490056 MS.gene55887:CDS
ATGGTGAATGCTGAGAAGAG+AGG 0.672519 4.3:+3489222 MS.gene55887:CDS
GTTGTGATTACAACTCCTGA+TGG 0.673437 4.3:+3490356 MS.gene55887:CDS
GTGATCCTCCCCACTCTATG+GGG 0.676832 4.3:-3490041 None:intergenic
TGTATGATCCCCATAGAGTG+GGG 0.683001 4.3:+3490033 MS.gene55887:CDS
ATGATCCCCATAGAGTGGGG+AGG 0.698167 4.3:+3490036 MS.gene55887:CDS
CTTCAAATCATGAAACACCG+CGG 0.732704 4.3:+3489294 MS.gene55887:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACACTAAATAAAAACAAAA+CGG - chr4.3:3490191-3490210 None:intergenic 15.0%
!!! AGAATTTAAAATATAAAAGC+TGG - chr4.3:3490263-3490282 None:intergenic 15.0%
!!! TTTTTAATAGATATGAGTAA+AGG + chr4.3:3490286-3490305 MS.gene55887:intron 15.0%
!! ATGTCTTAATTAATGGAATT+TGG - chr4.3:3489465-3489484 None:intergenic 20.0%
!! CATTAATTAAGACATACATT+AGG + chr4.3:3489470-3489489 MS.gene55887:intron 20.0%
!! CTAATGTATGTCTTAATTAA+TGG - chr4.3:3489472-3489491 None:intergenic 20.0%
!! TGTCTTAATTAATGGAATTT+GGG - chr4.3:3489464-3489483 None:intergenic 20.0%
!!! TTGTTTTTCTTACTTCTTAT+GGG + chr4.3:3489544-3489563 MS.gene55887:intron 20.0%
! AAGAAAAGAAGAGATAACAT+TGG - chr4.3:3489277-3489296 None:intergenic 25.0%
! AATTCAAACAAATATTACCG+CGG - chr4.3:3489314-3489333 None:intergenic 25.0%
! ATAGATATGAGTAAAGGAAA+AGG + chr4.3:3490292-3490311 MS.gene55887:intron 25.0%
! ATTTCATACTCATAAGATGA+AGG - chr4.3:3489365-3489384 None:intergenic 25.0%
! CATAAGAAGTAAGAAAAACA+AGG - chr4.3:3489545-3489564 None:intergenic 25.0%
! TAATTGTTGGAAAATAACCA+TGG - chr4.3:3489861-3489880 None:intergenic 25.0%
! TCATATCTCATGAAAAGAAA+TGG + chr4.3:3489832-3489851 MS.gene55887:intron 25.0%
! TTACTTCCATTATCTCAAAT+TGG + chr4.3:3489763-3489782 MS.gene55887:intron 25.0%
! TTTATTTATCTTGATGCAGT+GGG + chr4.3:3490328-3490347 MS.gene55887:intron 25.0%
! TTTCATACTCATAAGATGAA+GGG - chr4.3:3489364-3489383 None:intergenic 25.0%
!!! CTTGTTTTTCTTACTTCTTA+TGG + chr4.3:3489543-3489562 MS.gene55887:intron 25.0%
!!! TGTGTTTTGGATAATTCATT+AGG + chr4.3:3489414-3489433 MS.gene55887:intron 25.0%
!!! TTTTATTTATCTTGATGCAG+TGG + chr4.3:3490327-3490346 MS.gene55887:intron 25.0%
AAAACAATGGAGAGAGAAAA+AGG + chr4.3:3489570-3489589 MS.gene55887:intron 30.0%
ACAAATATAATCACGTGTGT+AGG - chr4.3:3489743-3489762 None:intergenic 30.0%
AGCATGTATATGTACATGAA+AGG + chr4.3:3489917-3489936 MS.gene55887:intron 30.0%
AGCATTCTCCATATACAAAT+AGG - chr4.3:3490226-3490245 None:intergenic 30.0%
CAGTTTCTCCTATTTGTATA+TGG + chr4.3:3490215-3490234 MS.gene55887:intron 30.0%
GAAGAGATAACATTGGAAAT+AGG - chr4.3:3489270-3489289 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG - chr4.3:3489519-3489538 None:intergenic 30.0%
TTATTTATCTTGATGCAGTG+GGG + chr4.3:3490329-3490348 MS.gene55887:intron 30.0%
TTCTTATGGGTCAAAAACAA+TGG + chr4.3:3489557-3489576 MS.gene55887:intron 30.0%
! ATTTTCCAACAATTATCAGC+TGG + chr4.3:3489866-3489885 MS.gene55887:intron 30.0%
! GTCATACCAATTTGAGATAA+TGG - chr4.3:3489772-3489791 None:intergenic 30.0%
!!! AACCACTTTTTGACAACTTT+TGG - chr4.3:3489642-3489661 None:intergenic 30.0%
!!! TATGTATTCGTTTTTTGAGC+TGG + chr4.3:3489386-3489405 MS.gene55887:intron 30.0%
AATTATCAGCTGGATAAGCA+TGG + chr4.3:3489876-3489895 MS.gene55887:intron 35.0%
AGGATATATCAAGGACTTTC+AGG + chr4.3:3490010-3490029 MS.gene55887:intron 35.0%
AGGTAGAATTAACGATTGCA+AGG + chr4.3:3490076-3490095 MS.gene55887:CDS 35.0%
ATGAAAAGAAATGGTCTCCA+TGG + chr4.3:3489841-3489860 MS.gene55887:intron 35.0%
ATTCAATATCCTTTGCCTTG+AGG - chr4.3:3490121-3490140 None:intergenic 35.0%
CTTATCCAGCTGATAATTGT+TGG - chr4.3:3489874-3489893 None:intergenic 35.0%
GTCCAAAAGTTGTCAAAAAG+TGG + chr4.3:3489637-3489656 MS.gene55887:intron 35.0%
! TGAGCTGGATTTATGTGTTT+TGG + chr4.3:3489401-3489420 MS.gene55887:intron 35.0%
!! TAATTTGCTGTCGTTCATGT+TGG + chr4.3:3489986-3490005 MS.gene55887:intron 35.0%
AAAAAGGAATGTAGGTGGTC+AGG + chr4.3:3490403-3490422 MS.gene55887:CDS 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG + chr4.3:3489580-3489599 MS.gene55887:intron 40.0%
AGGATCACAGTTGAACTACA+AGG + chr4.3:3490056-3490075 MS.gene55887:CDS 40.0%
CATGTTGGCAGGATATATCA+AGG + chr4.3:3490001-3490020 MS.gene55887:intron 40.0%
CATTAACCAACCTGATGTGT+TGG - chr4.3:3490164-3490183 None:intergenic 40.0%
CTCAAATTGGTATGACGTTG+AGG + chr4.3:3489776-3489795 MS.gene55887:intron 40.0%
CTTCAAATCATGAAACACCG+CGG + chr4.3:3489294-3489313 MS.gene55887:CDS 40.0%
GTTGTGATTACAACTCCTGA+TGG + chr4.3:3490356-3490375 MS.gene55887:CDS 40.0%
TCATGATCCAAAACACCATC+AGG - chr4.3:3490374-3490393 None:intergenic 40.0%
! ACCACCTACATTCCTTTTTG+CGG - chr4.3:3490402-3490421 None:intergenic 40.0%
!! TACAACTCCTGATGGTGTTT+TGG + chr4.3:3490364-3490383 MS.gene55887:CDS 40.0%
!! TGGTGTTTTGGATCATGAAG+AGG + chr4.3:3490376-3490395 MS.gene55887:CDS 40.0%
AATGTAGGTGGTCAGGTTCT+TGG + chr4.3:3490410-3490429 MS.gene55887:CDS 45.0%
ACTCTTCCAACACATCAGGT+TGG + chr4.3:3490155-3490174 MS.gene55887:intron 45.0%
ATCATGAAGAGGCCGCAAAA+AGG + chr4.3:3490387-3490406 MS.gene55887:CDS 45.0%
ATGGTGAATGCTGAGAAGAG+AGG + chr4.3:3489222-3489241 MS.gene55887:CDS 45.0%
ATTGGTATGACGTTGAGGTG+TGG + chr4.3:3489781-3489800 MS.gene55887:intron 45.0%
GAACACTCTTCCAACACATC+AGG + chr4.3:3490151-3490170 MS.gene55887:CDS 45.0%
GTGTATGATCCCCATAGAGT+GGG + chr4.3:3490032-3490051 MS.gene55887:CDS 45.0%
TGAGAAGAGAGGCAAAGCTA+TGG + chr4.3:3489233-3489252 MS.gene55887:CDS 45.0%
TGTATGATCCCCATAGAGTG+GGG + chr4.3:3490033-3490052 MS.gene55887:CDS 45.0%
TTGGTATGACGTTGAGGTGT+GGG + chr4.3:3489782-3489801 MS.gene55887:intron 45.0%
GAACGATGCGTTGAGGAGTA+TGG + chr4.3:3489203-3489222 MS.gene55887:CDS 50.0%
GAAGAGAGGCAAAGCTATGG+TGG + chr4.3:3489236-3489255 MS.gene55887:CDS 50.0%
GAGGCCGCAAAAAGGAATGT+AGG + chr4.3:3490395-3490414 MS.gene55887:CDS 50.0%
GCCGCAAAAAGGAATGTAGG+TGG + chr4.3:3490398-3490417 MS.gene55887:CDS 50.0%
GGATACTGAACGATGCGTTG+AGG + chr4.3:3489196-3489215 MS.gene55887:CDS 50.0%
GGTGTATGATCCCCATAGAG+TGG + chr4.3:3490031-3490050 MS.gene55887:CDS 50.0%
TGTGATCCTCCCCACTCTAT+GGG - chr4.3:3490045-3490064 None:intergenic 50.0%
TTGCTGTCGTTCATGTTGGC+AGG + chr4.3:3489990-3490009 MS.gene55887:intron 50.0%
! CCTTTGCCTTGAGGTCTTGT+CGG - chr4.3:3490112-3490131 None:intergenic 50.0%
! GAGGTCTTGTCGGTACGTAA+GGG - chr4.3:3490102-3490121 None:intergenic 50.0%
! TACGTACCGACAAGACCTCA+AGG + chr4.3:3490103-3490122 MS.gene55887:CDS 50.0%
! TGAGGTCTTGTCGGTACGTA+AGG - chr4.3:3490103-3490122 None:intergenic 50.0%
ATGATCCCCATAGAGTGGGG+AGG + chr4.3:3490036-3490055 MS.gene55887:CDS 55.0%
CCGACAAGACCTCAAGGCAA+AGG + chr4.3:3490109-3490128 MS.gene55887:CDS 55.0%
CTGTGATCCTCCCCACTCTA+TGG - chr4.3:3490046-3490065 None:intergenic 55.0%
GTGATCCTCCCCACTCTATG+GGG - chr4.3:3490044-3490063 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 3489186 3490445 3489186 ID=MS.gene55887
chr4.3 mRNA 3489186 3490445 3489186 ID=MS.gene55887.t1;Parent=MS.gene55887
chr4.3 exon 3489186 3489315 3489186 ID=MS.gene55887.t1.exon1;Parent=MS.gene55887.t1
chr4.3 CDS 3489186 3489315 3489186 ID=cds.MS.gene55887.t1;Parent=MS.gene55887.t1
chr4.3 exon 3490012 3490172 3490012 ID=MS.gene55887.t1.exon2;Parent=MS.gene55887.t1
chr4.3 CDS 3490012 3490172 3490012 ID=cds.MS.gene55887.t1;Parent=MS.gene55887.t1
chr4.3 exon 3490347 3490445 3490347 ID=MS.gene55887.t1.exon3;Parent=MS.gene55887.t1
chr4.3 CDS 3490347 3490445 3490347 ID=cds.MS.gene55887.t1;Parent=MS.gene55887.t1
Gene Sequence

>MS.gene55887

ATGGGGAGAAGGATACTGAACGATGCGTTGAGGAGTATGGTGAATGCTGAGAAGAGAGGCAAAGCTATGGTGGAGCTTAAACCTATTTCCAATGTTATCTCTTCTTTTCTTCAAATCATGAAACACCGCGGTAATATTTGTTTGAATTCTTTCATTATTTATTTTCTTTTATTTTCCCTTCATCTTATGAGTATGAAATTTATGTATTCGTTTTTTGAGCTGGATTTATGTGTTTTGGATAATTCATTAGGATATTGTTATTGTTTTAGTAAATACCCAAATTCCATTAATTAAGACATACATTAGGATAGAGTAATGATATTTTGACATCCATTTGATGACAACTTTGTTGACAACCTTGTTTTTCTTACTTCTTATGGGTCAAAAACAATGGAGAGAGAAAAAGGAAGAGAGAGGATAACAACATCATGTGAGTATGAGAGAGAAAGTTGTCCAAAAGTTGTCAAAAAGTGGTTGTCAAAATATCATTTCTCTTTCTCAAGAACTATGTAGCATATACACTTTAGATTGAAGACATGTTTAGTGTCTAGACACCTACACACGTGATTATATTTGTTTACTTCCATTATCTCAAATTGGTATGACGTTGAGGTGTGGGTGCAGTTGTCGTGTTTTCGTCAGTGCTTCATATCTCATGAAAAGAAATGGTCTCCATGGTTATTTTCCAACAATTATCAGCTGGATAAGCATGGTCTTGCTTTCTGTATGTCAGCATGTATATGTACATGAAAGGCACTAAACGTACTAGAATAATTTGATATTTAGATGATCGCTACAGTTAATTTGCTGTCGTTCATGTTGGCAGGATATATCAAGGACTTTCAGGTGTATGATCCCCATAGAGTGGGGAGGATCACAGTTGAACTACAAGGTAGAATTAACGATTGCAAGGCCCTTACGTACCGACAAGACCTCAAGGCAAAGGATATTGAATCTTACAGATTGAACACTCTTCCAACACATCAGGTTGGTTAATGCTTACCGTTTTGTTTTTATTTAGTGTTATAGCAGTTTCTCCTATTTGTATATGGAGAATGCTGTTTGTTATAGCTGCCAGCTTTTATATTTTAAATTCTTTCTTTTTAATAGATATGAGTAAAGGAAAAGGATATTCATTTCATTTTATTTATCTTGATGCAGTGGGGTTATGTTGTGATTACAACTCCTGATGGTGTTTTGGATCATGAAGAGGCCGCAAAAAGGAATGTAGGTGGTCAGGTTCTTGGTTACTTTCACTAA

Protein sequence

>MS.gene55887.t1

MGRRILNDALRSMVNAEKRGKAMVELKPISNVISSFLQIMKHRGYIKDFQVYDPHRVGRITVELQGRINDCKALTYRQDLKAKDIESYRLNTLPTHQWGYVVITTPDGVLDHEEAAKRNVGGQVLGYFH