AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06172


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06172.t1 MTR_8g101910 98.450 129 2 0 1 129 1 129 4.67e-94 267
MS.gene06172.t1 MTR_8g101910 96.970 99 3 0 1 99 1 99 3.41e-68 201
MS.gene06172.t1 MTR_4g094598 52.000 125 60 0 5 129 6 130 7.77e-46 145
MS.gene06172.t1 MTR_1g105415 52.000 125 60 0 5 129 6 130 7.77e-46 145
MS.gene06172.t1 MTR_4g117470 52.000 125 60 0 5 129 6 130 1.99e-45 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06172.t1 AT4G29430 81.395 129 24 0 1 129 1 129 5.77e-79 229
MS.gene06172.t1 AT2G19720 79.845 129 26 0 1 129 1 129 1.60e-77 225
MS.gene06172.t1 AT2G39590 52.000 125 60 0 5 129 6 130 3.50e-46 146
MS.gene06172.t1 AT5G59850 52.000 125 60 0 5 129 6 130 1.97e-45 144
MS.gene06172.t1 AT1G07770 52.000 125 60 0 5 129 6 130 1.97e-45 144
MS.gene06172.t1 AT1G07770 52.000 125 60 0 5 129 6 130 1.97e-45 144
MS.gene06172.t1 AT1G07770 52.000 125 60 0 5 129 6 130 1.97e-45 144
MS.gene06172.t1 AT3G46040 50.400 125 62 0 5 129 6 130 2.76e-44 141

Find 33 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTGATCCTCCCCACTCTA+TGG 0.263185 4.2:-3285826 None:intergenic
GAAGAGATAACATTGGAAAT+AGG 0.265767 4.2:-3285161 None:intergenic
TACAACTCCTGATGGTGTTT+TGG 0.307095 4.2:+3286150 MS.gene06172:CDS
AATGTAGGTGGTCAGGTTCT+TGG 0.331557 4.2:+3286196 MS.gene06172:CDS
AGGATATATCAAGGACTTTC+AGG 0.389863 4.2:+3285793 MS.gene06172:intron
CCTTTGCCTTGAGGTCTTGT+CGG 0.398017 4.2:-3285892 None:intergenic
ATCATGAAGAGGCCGCAAAA+AGG 0.404793 4.2:+3286173 MS.gene06172:CDS
TGAGGTCTTGTCGGTACGTA+AGG 0.430606 4.2:-3285883 None:intergenic
TGTGATCCTCCCCACTCTAT+GGG 0.457890 4.2:-3285825 None:intergenic
GAACGATGCGTTGAGGAGTA+TGG 0.475978 4.2:+3285097 MS.gene06172:CDS
GAACACTCTTCCAACACATC+AGG 0.488180 4.2:+3285934 MS.gene06172:CDS
TCATGATCCAAAACACCATC+AGG 0.488311 4.2:-3286157 None:intergenic
AAGAAAAGAAGAGATAACAT+TGG 0.493444 4.2:-3285168 None:intergenic
GAGAAAGAGAAATGGGGAGA+AGG 0.505773 4.2:+3285069 None:intergenic
CATTAACCAACCTGATGTGT+TGG 0.507241 4.2:-3285944 None:intergenic
ATTCAATATCCTTTGCCTTG+AGG 0.544391 4.2:-3285901 None:intergenic
GAAGAGAGGCAAAGCTATGG+TGG 0.562394 4.2:+3285130 MS.gene06172:CDS
GTGTATGATCCCCATAGAGT+GGG 0.576109 4.2:+3285815 MS.gene06172:CDS
CATGTTGGCAGGATATATCA+AGG 0.581279 4.2:+3285784 MS.gene06172:intron
GAGGTCTTGTCGGTACGTAA+GGG 0.585892 4.2:-3285882 None:intergenic
TGAGAAGAGAGGCAAAGCTA+TGG 0.613395 4.2:+3285127 MS.gene06172:CDS
GGTGTATGATCCCCATAGAG+TGG 0.614660 4.2:+3285814 MS.gene06172:CDS
AGGTAGAATTAACGATTGCA+AGG 0.617470 4.2:+3285859 MS.gene06172:CDS
TACGTACCGACAAGACCTCA+AGG 0.647236 4.2:+3285886 MS.gene06172:CDS
CCGACAAGACCTCAAGGCAA+AGG 0.651183 4.2:+3285892 MS.gene06172:CDS
GGATACTGAACGATGCGTTG+AGG 0.657571 4.2:+3285090 MS.gene06172:CDS
AGGATCACAGTTGAACTACA+AGG 0.660202 4.2:+3285839 MS.gene06172:CDS
ATGGTGAATGCTGAGAAGAG+AGG 0.672519 4.2:+3285116 MS.gene06172:CDS
GTTGTGATTACAACTCCTGA+TGG 0.673437 4.2:+3286142 MS.gene06172:CDS
GTGATCCTCCCCACTCTATG+GGG 0.676832 4.2:-3285824 None:intergenic
TGTATGATCCCCATAGAGTG+GGG 0.683001 4.2:+3285816 MS.gene06172:CDS
ATGATCCCCATAGAGTGGGG+AGG 0.698167 4.2:+3285819 MS.gene06172:CDS
CTTCAAATCATGAAACACCG+CGG 0.732704 4.2:+3285188 MS.gene06172:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTAAAGATATTTTTAG+TGG + chr4.2:3285423-3285442 MS.gene06172:intron 10.0%
!! AACACTAAATAAAAACAAAA+CGG - chr4.2:3285974-3285993 None:intergenic 15.0%
!!! AGAATTTAAAATATAAAAGC+TGG - chr4.2:3286046-3286065 None:intergenic 15.0%
!! ATGTCTTAATTAATGGAATT+TGG - chr4.2:3285359-3285378 None:intergenic 20.0%
!! CATTAATTAAGACATACATT+AGG + chr4.2:3285364-3285383 MS.gene06172:intron 20.0%
!! CTAATGTATGTCTTAATTAA+TGG - chr4.2:3285366-3285385 None:intergenic 20.0%
!! TGTCTTAATTAATGGAATTT+GGG - chr4.2:3285358-3285377 None:intergenic 20.0%
!! TTACTAATAGATATGAGTAA+AGG + chr4.2:3286072-3286091 MS.gene06172:intron 20.0%
! AAGAAAAGAAGAGATAACAT+TGG - chr4.2:3285171-3285190 None:intergenic 25.0%
! AATTCAAACAAATATTACCG+CGG - chr4.2:3285208-3285227 None:intergenic 25.0%
! ATAGATATGAGTAAAGGAAA+AGG + chr4.2:3286078-3286097 MS.gene06172:intron 25.0%
! ATTTCATACTCATAAGATGA+AGG - chr4.2:3285259-3285278 None:intergenic 25.0%
! TAATTGTTGGAAAATAACCA+TGG - chr4.2:3285644-3285663 None:intergenic 25.0%
! TCATATCTCATGAAAAGAAA+TGG + chr4.2:3285615-3285634 MS.gene06172:intron 25.0%
! TTACTTCCATTATCTCAAAT+TGG + chr4.2:3285546-3285565 MS.gene06172:intron 25.0%
! TTTATTTATCTTGATGCAGT+GGG + chr4.2:3286114-3286133 MS.gene06172:intron 25.0%
! TTTCATACTCATAAGATGAA+GGG - chr4.2:3285258-3285277 None:intergenic 25.0%
!!! TGTGTTTTGGATAATTCATT+AGG + chr4.2:3285308-3285327 MS.gene06172:intron 25.0%
!!! TTTTATTTATCTTGATGCAG+TGG + chr4.2:3286113-3286132 MS.gene06172:intron 25.0%
ACAAATATAATCACGTGTGT+AGG - chr4.2:3285526-3285545 None:intergenic 30.0%
AGCATGTATATGTACATGAA+AGG + chr4.2:3285700-3285719 MS.gene06172:intron 30.0%
AGCATTCTCCATTTACAAAT+AGG - chr4.2:3286009-3286028 None:intergenic 30.0%
CAGTTTCACCTATTTGTAAA+TGG + chr4.2:3285998-3286017 MS.gene06172:intron 30.0%
GAAGAGATAACATTGGAAAT+AGG - chr4.2:3285164-3285183 None:intergenic 30.0%
TAGTTCTTGAGAATGAGAAA+TGG - chr4.2:3285457-3285476 None:intergenic 30.0%
TTATTTATCTTGATGCAGTG+GGG + chr4.2:3286115-3286134 MS.gene06172:intron 30.0%
! ATTTTCCAACAATTATCAGC+TGG + chr4.2:3285649-3285668 MS.gene06172:intron 30.0%
! GTCATACCAATTTGAGATAA+TGG - chr4.2:3285555-3285574 None:intergenic 30.0%
!!! TATGTATTCGTTTTTTGAGC+TGG + chr4.2:3285280-3285299 MS.gene06172:intron 30.0%
AATTATCAGCTGGATAAGCA+TGG + chr4.2:3285659-3285678 MS.gene06172:intron 35.0%
AGGATATATCAAGGACTTTC+AGG + chr4.2:3285793-3285812 MS.gene06172:intron 35.0%
AGGTAGAATTAACGATTGCA+AGG + chr4.2:3285859-3285878 MS.gene06172:CDS 35.0%
ATGAAAAGAAATGGTCTCCA+TGG + chr4.2:3285624-3285643 MS.gene06172:intron 35.0%
ATTCAATATCCTTTGCCTTG+AGG - chr4.2:3285904-3285923 None:intergenic 35.0%
CTTATCCAGCTGATAATTGT+TGG - chr4.2:3285657-3285676 None:intergenic 35.0%
! TGAGCTGGATTTATGTGTTT+TGG + chr4.2:3285295-3285314 MS.gene06172:intron 35.0%
!! TAATTTGCTGTCGTTCATGT+TGG + chr4.2:3285769-3285788 MS.gene06172:intron 35.0%
AAAAAGGAATGTAGGTGGTC+AGG + chr4.2:3286189-3286208 MS.gene06172:CDS 40.0%
AGGATCACAGTTGAACTACA+AGG + chr4.2:3285839-3285858 MS.gene06172:CDS 40.0%
CATGTTGGCAGGATATATCA+AGG + chr4.2:3285784-3285803 MS.gene06172:intron 40.0%
CATTAACCAACCTGATGTGT+TGG - chr4.2:3285947-3285966 None:intergenic 40.0%
CTCAAATTGGTATGACGTTG+AGG + chr4.2:3285559-3285578 MS.gene06172:intron 40.0%
CTTCAAATCATGAAACACCG+CGG + chr4.2:3285188-3285207 MS.gene06172:CDS 40.0%
GTTGTGATTACAACTCCTGA+TGG + chr4.2:3286142-3286161 MS.gene06172:CDS 40.0%
TCATGATCCAAAACACCATC+AGG - chr4.2:3286160-3286179 None:intergenic 40.0%
! ACCACCTACATTCCTTTTTG+CGG - chr4.2:3286188-3286207 None:intergenic 40.0%
!! TACAACTCCTGATGGTGTTT+TGG + chr4.2:3286150-3286169 MS.gene06172:CDS 40.0%
!! TGGTGTTTTGGATCATGAAG+AGG + chr4.2:3286162-3286181 MS.gene06172:CDS 40.0%
AATGTAGGTGGTCAGGTTCT+TGG + chr4.2:3286196-3286215 MS.gene06172:CDS 45.0%
ACTCTTCCAACACATCAGGT+TGG + chr4.2:3285938-3285957 MS.gene06172:intron 45.0%
ATCATGAAGAGGCCGCAAAA+AGG + chr4.2:3286173-3286192 MS.gene06172:CDS 45.0%
ATGGTGAATGCTGAGAAGAG+AGG + chr4.2:3285116-3285135 MS.gene06172:CDS 45.0%
ATTGGTATGACGTTGAGGTG+TGG + chr4.2:3285564-3285583 MS.gene06172:intron 45.0%
GAACACTCTTCCAACACATC+AGG + chr4.2:3285934-3285953 MS.gene06172:CDS 45.0%
GTGTATGATCCCCATAGAGT+GGG + chr4.2:3285815-3285834 MS.gene06172:CDS 45.0%
TGAGAAGAGAGGCAAAGCTA+TGG + chr4.2:3285127-3285146 MS.gene06172:CDS 45.0%
TGTATGATCCCCATAGAGTG+GGG + chr4.2:3285816-3285835 MS.gene06172:CDS 45.0%
TTGGTATGACGTTGAGGTGT+GGG + chr4.2:3285565-3285584 MS.gene06172:intron 45.0%
!!! TATTATTAATTTTAGATTTT+TGG + chr4.2:3285388-3285407 MS.gene06172:intron 5.0%
GAACGATGCGTTGAGGAGTA+TGG + chr4.2:3285097-3285116 MS.gene06172:CDS 50.0%
GAAGAGAGGCAAAGCTATGG+TGG + chr4.2:3285130-3285149 MS.gene06172:CDS 50.0%
GAGGCCGCAAAAAGGAATGT+AGG + chr4.2:3286181-3286200 MS.gene06172:CDS 50.0%
GCCGCAAAAAGGAATGTAGG+TGG + chr4.2:3286184-3286203 MS.gene06172:CDS 50.0%
GGATACTGAACGATGCGTTG+AGG + chr4.2:3285090-3285109 MS.gene06172:CDS 50.0%
GGTGTATGATCCCCATAGAG+TGG + chr4.2:3285814-3285833 MS.gene06172:CDS 50.0%
TGTGATCCTCCCCACTCTAT+GGG - chr4.2:3285828-3285847 None:intergenic 50.0%
TTGCTGTCGTTCATGTTGGC+AGG + chr4.2:3285773-3285792 MS.gene06172:intron 50.0%
! CCTTTGCCTTGAGGTCTTGT+CGG - chr4.2:3285895-3285914 None:intergenic 50.0%
! GAGGTCTTGTCGGTACGTAA+GGG - chr4.2:3285885-3285904 None:intergenic 50.0%
! GGGTGCAGTTGTCGTGTTTT+CGG + chr4.2:3285585-3285604 MS.gene06172:intron 50.0%
! TACGTACCGACAAGACCTCA+AGG + chr4.2:3285886-3285905 MS.gene06172:CDS 50.0%
! TGAGGTCTTGTCGGTACGTA+AGG - chr4.2:3285886-3285905 None:intergenic 50.0%
ATGATCCCCATAGAGTGGGG+AGG + chr4.2:3285819-3285838 MS.gene06172:CDS 55.0%
CCGACAAGACCTCAAGGCAA+AGG + chr4.2:3285892-3285911 MS.gene06172:CDS 55.0%
CTGTGATCCTCCCCACTCTA+TGG - chr4.2:3285829-3285848 None:intergenic 55.0%
GTGATCCTCCCCACTCTATG+GGG - chr4.2:3285827-3285846 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 3285080 3286231 3285080 ID=MS.gene06172
chr4.2 mRNA 3285080 3286231 3285080 ID=MS.gene06172.t1;Parent=MS.gene06172
chr4.2 exon 3285080 3285209 3285080 ID=MS.gene06172.t1.exon1;Parent=MS.gene06172.t1
chr4.2 CDS 3285080 3285209 3285080 ID=cds.MS.gene06172.t1;Parent=MS.gene06172.t1
chr4.2 exon 3285795 3285955 3285795 ID=MS.gene06172.t1.exon2;Parent=MS.gene06172.t1
chr4.2 CDS 3285795 3285955 3285795 ID=cds.MS.gene06172.t1;Parent=MS.gene06172.t1
chr4.2 exon 3286133 3286231 3286133 ID=MS.gene06172.t1.exon3;Parent=MS.gene06172.t1
chr4.2 CDS 3286133 3286231 3286133 ID=cds.MS.gene06172.t1;Parent=MS.gene06172.t1
Gene Sequence

>MS.gene06172

ATGGGGAGAAGGATACTGAACGATGCGTTGAGGAGTATGGTGAATGCTGAGAAGAGAGGCAAAGCTATGGTGGAGCTTAAACCTATTTCCAATGTTATCTCTTCTTTTCTTCAAATCATGAAACACCGCGGTAATATTTGTTTGAATTCTTTCATTATTTATTTTCTTTTATTTTCCCTTCATCTTATGAGTATGAAATTTATGTATTCGTTTTTTGAGCTGGATTTATGTGTTTTGGATAATTCATTAGGATATTGTTATTGTTTTAGTAAATACCCAAATTCCATTAATTAAGACATACATTAGGATATTATTAATTTTAGATTTTTGGCATATTAGTATAATTTTTAAAGATATTTTTAGTGGTATTTTATCCATTTCTCATTCTCAAGAACTATGTAGCGTATACACTTTAGATTGAAGACATGTTTAGTGTCTAGACACCTACACACGTGATTATATTTGTTTACTTCCATTATCTCAAATTGGTATGACGTTGAGGTGTGGGTGCAGTTGTCGTGTTTTCGGCAGTGCTTCATATCTCATGAAAAGAAATGGTCTCCATGGTTATTTTCCAACAATTATCAGCTGGATAAGCATGGTCTTGCTTTCTGTATGTCAGCATGTATATGTACATGAAAGGCACTAAACGTACTAGAATAATTTGATATTTAGATAATCGCTACAGTTAATTTGCTGTCGTTCATGTTGGCAGGATATATCAAGGACTTTCAGGTGTATGATCCCCATAGAGTGGGGAGGATCACAGTTGAACTACAAGGTAGAATTAACGATTGCAAGGCCCTTACGTACCGACAAGACCTCAAGGCAAAGGATATTGAATCTTACAGATTGAACACTCTTCCAACACATCAGGTTGGTTAATGCTTACCGTTTTGTTTTTATTTAGTGTTATAGCAGTTTCACCTATTTGTAAATGGAGAATGCTGTTTGTTATAGCTGCCAGCTTTTATATTTTAAATTCTTTCTTTTTACTAATAGATATGAGTAAAGGAAAAGGATATTCATTTCATTTTATTTATCTTGATGCAGTGGGGTTATGTTGTGATTACAACTCCTGATGGTGTTTTGGATCATGAAGAGGCCGCAAAAAGGAATGTAGGTGGTCAGGTTCTTGGTTACTTTCACTAA

Protein sequence

>MS.gene06172.t1

MGRRILNDALRSMVNAEKRGKAMVELKPISNVISSFLQIMKHRGYIKDFQVYDPHRVGRITVELQGRINDCKALTYRQDLKAKDIESYRLNTLPTHQWGYVVITTPDGVLDHEEAAKRNVGGQVLGYFH