AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58227


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58227.t1 MTR_4g117470 100.000 130 0 0 1 130 1 130 1.05e-93 266
MS.gene58227.t1 MTR_4g094598 99.231 130 1 0 1 130 1 130 2.82e-93 265
MS.gene58227.t1 MTR_1g105415 99.231 130 1 0 1 130 1 130 2.82e-93 265
MS.gene58227.t1 MTR_8g101910 52.000 125 60 0 6 130 5 129 1.82e-45 144
MS.gene58227.t1 MTR_8g101910 49.474 95 48 0 6 100 5 99 1.72e-28 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58227.t1 AT5G59850 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene58227.t1 AT1G07770 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene58227.t1 AT1G07770 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene58227.t1 AT1G07770 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene58227.t1 AT3G46040 97.692 130 3 0 1 130 1 130 4.03e-92 262
MS.gene58227.t1 AT2G39590 91.538 130 11 0 1 130 1 130 4.30e-87 249
MS.gene58227.t1 AT4G29430 54.400 125 57 0 6 130 5 129 2.98e-47 149
MS.gene58227.t1 AT2G19720 53.600 125 58 0 6 130 5 129 3.11e-45 144

Find 30 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCACTTCTGCTGGTATTA+TGG 0.215449 8.3:+8397577 MS.gene58227:CDS
TTATGCTTTATAGGTTATAT+TGG 0.301097 8.3:+8396370 MS.gene58227:intron
TATGATTAGGCCTTCCTCTA+AGG 0.335085 8.3:+8396168 MS.gene58227:CDS
GAATTTGATGATAACCTTAG+AGG 0.407415 8.3:-8396182 None:intergenic
GAAAGCGTCAAGTTATGATT+AGG 0.452540 8.3:+8396155 MS.gene58227:CDS
TATGTTGATGATCACAGATC+TGG 0.452973 8.3:+8396403 MS.gene58227:CDS
GGTCCTTGGCTTCTTCTACT+AGG 0.468896 8.3:+8397637 MS.gene58227:CDS
AATGTTGGTGGTAAGGTCCT+TGG 0.482167 8.3:+8397623 MS.gene58227:CDS
AAAATCGTTGTTGAATTGAA+TGG 0.485579 8.3:+8396427 MS.gene58227:CDS
GAGAAAGAATGTTGGTGGTA+AGG 0.495772 8.3:+8397616 MS.gene58227:CDS
ATGGTAGGTTGAACAAATGT+GGG 0.519393 8.3:+8396446 MS.gene58227:CDS
ATCCATAATACCAGCAGAAG+TGG 0.531152 8.3:-8397579 None:intergenic
TAGACTTCTCCCTTCCAGAC+AGG 0.537117 8.3:+8396522 MS.gene58227:CDS
ATGTACAATGCTGAGAAAAG+AGG 0.544704 8.3:+8396133 MS.gene58227:CDS
TTCAACTGACCTGTCTGGAA+GGG 0.545440 8.3:-8396531 None:intergenic
TTTCAACTGACCTGTCTGGA+AGG 0.549780 8.3:-8396532 None:intergenic
ATGTTGATGATCACAGATCT+GGG 0.565212 8.3:+8396404 MS.gene58227:CDS
GCTAGGAGAAAGAATGTTGG+TGG 0.567141 8.3:+8397611 MS.gene58227:CDS
TAGCCTAGTAGAAGAAGCCA+AGG 0.575489 8.3:-8397640 None:intergenic
AATGGTAGGTTGAACAAATG+TGG 0.577364 8.3:+8396445 MS.gene58227:CDS
GAGGCTAGGAGAAAGAATGT+TGG 0.584494 8.3:+8397608 MS.gene58227:CDS
TTATGGATCATGAAGAGGCT+AGG 0.585160 8.3:+8397594 MS.gene58227:CDS
TTGATGATAACCTTAGAGGA+AGG 0.606187 8.3:-8396178 None:intergenic
TGGTATTATGGATCATGAAG+AGG 0.606467 8.3:+8397589 MS.gene58227:CDS
GTCGGTGTCAAGGAGATTGA+AGG 0.613514 8.3:+8396490 MS.gene58227:CDS
TCGTTGTTGAATTGAATGGT+AGG 0.614641 8.3:+8396431 MS.gene58227:CDS
CAAAACACTGACTCTCACCA+TGG 0.638914 8.3:-8396092 None:intergenic
GTGTCAAGGAGATTGAAGGT+TGG 0.653545 8.3:+8396494 MS.gene58227:CDS
TGGTAGGTTGAACAAATGTG+GGG 0.708928 8.3:+8396447 MS.gene58227:CDS
TTGACACCGACATCAAAACG+AGG 0.734537 8.3:-8396478 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTAAATAATAACAATTTA+AGG - chr8.3:8397443-8397462 None:intergenic 10.0%
!! ATAATATAAAATATGCAGTT+TGG + chr8.3:8397542-8397561 MS.gene58227:intron 15.0%
!! CTAAAATTGATCATAAAAAA+CGG - chr8.3:8397320-8397339 None:intergenic 15.0%
!!! AACTTAATTTATCTTTTGAT+AGG + chr8.3:8396731-8396750 MS.gene58227:intron 15.0%
!!! TGAAAACAATACTAATTTTT+GGG + chr8.3:8396550-8396569 MS.gene58227:intron 15.0%
!!! TTGAAAACAATACTAATTTT+TGG + chr8.3:8396549-8396568 MS.gene58227:intron 15.0%
!!! TTGATTTGATATAATTTTGT+GGG + chr8.3:8396295-8396314 MS.gene58227:intron 15.0%
!!! TTTGATTTGATATAATTTTG+TGG + chr8.3:8396294-8396313 MS.gene58227:intron 15.0%
!!! TTTGATTTGATATATTTTGT+GGG + chr8.3:8396347-8396366 MS.gene58227:intron 15.0%
!!! TTTTGATTTGATATATTTTG+TGG + chr8.3:8396346-8396365 MS.gene58227:intron 15.0%
!! AAAACTAATAAATGTTGCAT+TGG - chr8.3:8396842-8396861 None:intergenic 20.0%
!! GGATGAAATTCATTAAATAA+GGG - chr8.3:8396879-8396898 None:intergenic 20.0%
!! TGGATGAAATTCATTAAATA+AGG - chr8.3:8396880-8396899 None:intergenic 20.0%
!! TTATGCTTTATAGGTTATAT+TGG + chr8.3:8396370-8396389 MS.gene58227:intron 20.0%
!! TTGCATGATTCAATTATTTA+GGG + chr8.3:8396270-8396289 MS.gene58227:intron 20.0%
!!! AAAAAACGGAGATTTTTTTT+AGG - chr8.3:8397306-8397325 None:intergenic 20.0%
!!! AGGAAAAAAAAAGATTTTAG+AGG - chr8.3:8396239-8396258 None:intergenic 20.0%
!!! ATTAAATAAGGGTATAATAG+TGG - chr8.3:8396868-8396887 None:intergenic 20.0%
!!! ATTAATTGTGTTGATTTCAA+TGG - chr8.3:8396963-8396982 None:intergenic 20.0%
!!! TTTTTTTTTCCTTTCATTGA+AGG + chr8.3:8396247-8396266 MS.gene58227:intron 20.0%
! AAAATCGTTGTTGAATTGAA+TGG + chr8.3:8396427-8396446 MS.gene58227:CDS 25.0%
! ATTGAACTTATATGAACGTT+GGG + chr8.3:8397469-8397488 MS.gene58227:intron 25.0%
! GTTGCATGATTCAATTATTT+AGG + chr8.3:8396269-8396288 MS.gene58227:intron 25.0%
! TATAGTTTCTTACTCCTATT+TGG + chr8.3:8397252-8397271 MS.gene58227:intron 25.0%
! TATTGAACTTATATGAACGT+TGG + chr8.3:8397468-8397487 MS.gene58227:intron 25.0%
! TCATGCTTCATATTAAAGAT+TGG + chr8.3:8397216-8397235 MS.gene58227:intron 25.0%
!! TATAATTTTGTGGGTATGTT+TGG + chr8.3:8396304-8396323 MS.gene58227:intron 25.0%
!! TGATTTGGAACAAATTTACT+CGG - chr8.3:8397189-8397208 None:intergenic 25.0%
!!! TCACAATATTAGTCGTTTTA+AGG - chr8.3:8397010-8397029 None:intergenic 25.0%
!!! TCAGCTTATTTTCATAAACA+TGG + chr8.3:8396682-8396701 MS.gene58227:intron 25.0%
!!! TTTGGTGGTTCAATTTTTTA+GGG + chr8.3:8396322-8396341 MS.gene58227:intron 25.0%
AAACGACTAATATTGTGAGA+CGG + chr8.3:8397012-8397031 MS.gene58227:intron 30.0%
AAGCATGAACTATCATGATT+TGG - chr8.3:8397204-8397223 None:intergenic 30.0%
AAGTTCACACAAATATGCAT+TGG - chr8.3:8397117-8397136 None:intergenic 30.0%
ACACCCTTATTAATTGTAGT+TGG - chr8.3:8396917-8396936 None:intergenic 30.0%
ATTTACTCGGCTTAACAAAA+TGG - chr8.3:8397176-8397195 None:intergenic 30.0%
CATAGCTATATGTAAGAACA+TGG - chr8.3:8397078-8397097 None:intergenic 30.0%
CCACCAACTACAATTAATAA+GGG + chr8.3:8396911-8396930 MS.gene58227:intron 30.0%
TCCACCAACTACAATTAATA+AGG + chr8.3:8396910-8396929 MS.gene58227:intron 30.0%
TTTGGGAGAACTTATGAAAA+CGG + chr8.3:8396634-8396653 MS.gene58227:intron 30.0%
! GAATTTGATGATAACCTTAG+AGG - chr8.3:8396185-8396204 None:intergenic 30.0%
!! TTTTGTGGGTTATGCTTTAT+AGG + chr8.3:8396361-8396380 MS.gene58227:intron 30.0%
!!! GTTTGGTGGTTCAATTTTTT+AGG + chr8.3:8396321-8396340 MS.gene58227:intron 30.0%
AAGGTTGTGCACGATTATTA+AGG - chr8.3:8396991-8397010 None:intergenic 35.0%
ACACAAATATGCATTGGAAG+TGG - chr8.3:8397111-8397130 None:intergenic 35.0%
AGACAATAATTGTGAGACAG+AGG - chr8.3:8396773-8396792 None:intergenic 35.0%
AGTAAGCTGTTTATCCAAAC+AGG + chr8.3:8397051-8397070 MS.gene58227:intron 35.0%
ATCATGCAACCTTCAATGAA+AGG - chr8.3:8396259-8396278 None:intergenic 35.0%
ATGTACAATGCTGAGAAAAG+AGG + chr8.3:8396133-8396152 MS.gene58227:CDS 35.0%
CCCTTATTAATTGTAGTTGG+TGG - chr8.3:8396914-8396933 None:intergenic 35.0%
CTCATCGTTATGCAAAAACA+CGG + chr8.3:8396205-8396224 MS.gene58227:CDS 35.0%
GAAACAACACTACACACAAA+AGG - chr8.3:8397500-8397519 None:intergenic 35.0%
GACAATAATTGTGAGACAGA+GGG - chr8.3:8396772-8396791 None:intergenic 35.0%
TATCACCTGTGAGAATGTTT+GGG + chr8.3:8396617-8396636 MS.gene58227:intron 35.0%
TCGTTGTTGAATTGAATGGT+AGG + chr8.3:8396431-8396450 MS.gene58227:CDS 35.0%
TGGTATTATGGATCATGAAG+AGG + chr8.3:8397589-8397608 MS.gene58227:CDS 35.0%
TTATCACCTGTGAGAATGTT+TGG + chr8.3:8396616-8396635 MS.gene58227:intron 35.0%
TTTGTCCACAAAGACCAAAT+AGG - chr8.3:8397269-8397288 None:intergenic 35.0%
! AATGGTAGGTTGAACAAATG+TGG + chr8.3:8396445-8396464 MS.gene58227:CDS 35.0%
! AATTTTGTGGGTATGTTTGG+TGG + chr8.3:8396307-8396326 MS.gene58227:intron 35.0%
! ATGGTAGGTTGAACAAATGT+GGG + chr8.3:8396446-8396465 MS.gene58227:CDS 35.0%
! ATTTGTTGCTTGGATGATCT+AGG + chr8.3:8397392-8397411 MS.gene58227:intron 35.0%
! TTACTCCTATTTGGTCTTTG+TGG + chr8.3:8397261-8397280 MS.gene58227:intron 35.0%
! TTGATGATAACCTTAGAGGA+AGG - chr8.3:8396181-8396200 None:intergenic 35.0%
!! ATGTTGATGATCACAGATCT+GGG + chr8.3:8396404-8396423 MS.gene58227:CDS 35.0%
!! ATTAGTCCTCGTTTTGATGT+CGG + chr8.3:8396472-8396491 MS.gene58227:CDS 35.0%
!! GAAAGCGTCAAGTTATGATT+AGG + chr8.3:8396155-8396174 MS.gene58227:CDS 35.0%
!! TATGTTGATGATCACAGATC+TGG + chr8.3:8396403-8396422 MS.gene58227:CDS 35.0%
!! TCAATTTTAGCCTCTACTAG+AGG + chr8.3:8397330-8397349 MS.gene58227:intron 35.0%
!! TCTGTCAACAATTTGTTGCT+TGG + chr8.3:8397382-8397401 MS.gene58227:intron 35.0%
ATCCATAATACCAGCAGAAG+TGG - chr8.3:8397582-8397601 None:intergenic 40.0%
CACATAAAAGCCTCTAGTAG+AGG - chr8.3:8397343-8397362 None:intergenic 40.0%
GACTAATATTGTGAGACGGA+GGG + chr8.3:8397016-8397035 MS.gene58227:intron 40.0%
TATGATTAGGCCTTCCTCTA+AGG + chr8.3:8396168-8396187 MS.gene58227:CDS 40.0%
TGTAAGAACATGGTCCTGTT+TGG - chr8.3:8397068-8397087 None:intergenic 40.0%
TTATGGATCATGAAGAGGCT+AGG + chr8.3:8397594-8397613 MS.gene58227:CDS 40.0%
! CTACTAGAGGCTTTTATGTG+TGG + chr8.3:8397343-8397362 MS.gene58227:intron 40.0%
! CTTCAGTGTTATCGATTGCA+TGG + chr8.3:8397147-8397166 MS.gene58227:intron 40.0%
! TGGTAGGTTGAACAAATGTG+GGG + chr8.3:8396447-8396466 MS.gene58227:CDS 40.0%
! TTCTTTTGCATAGTGTCTGC+TGG + chr8.3:8396576-8396595 MS.gene58227:intron 40.0%
! TTGTTTTCAACTGACCTGTC+TGG - chr8.3:8396539-8396558 None:intergenic 40.0%
!! AGTTGGTGGAGTAGTTAAGT+TGG - chr8.3:8396900-8396919 None:intergenic 40.0%
!! CGTGTTTTTGCATAACGATG+AGG - chr8.3:8396207-8396226 None:intergenic 40.0%
!! GAGAAAGAATGTTGGTGGTA+AGG + chr8.3:8397616-8397635 MS.gene58227:CDS 40.0%
!!! ATTGTTTTGACCACTTCTGC+TGG + chr8.3:8397569-8397588 MS.gene58227:CDS 40.0%
CGACTAATATTGTGAGACGG+AGG + chr8.3:8397015-8397034 MS.gene58227:intron 45.0%
GACCACTTCTGCTGGTATTA+TGG + chr8.3:8397577-8397596 MS.gene58227:CDS 45.0%
GAGGCTAGGAGAAAGAATGT+TGG + chr8.3:8397608-8397627 MS.gene58227:CDS 45.0%
GCTAGGAGAAAGAATGTTGG+TGG + chr8.3:8397611-8397630 MS.gene58227:CDS 45.0%
GTGTCAAGGAGATTGAAGGT+TGG + chr8.3:8396494-8396513 MS.gene58227:CDS 45.0%
GTTCTCCCAAACATTCTCAC+AGG - chr8.3:8396625-8396644 None:intergenic 45.0%
TTCAACTGACCTGTCTGGAA+GGG - chr8.3:8396534-8396553 None:intergenic 45.0%
TTGACACCGACATCAAAACG+AGG - chr8.3:8396481-8396500 None:intergenic 45.0%
TTTCAACTGACCTGTCTGGA+AGG - chr8.3:8396535-8396554 None:intergenic 45.0%
!! AATGTTGGTGGTAAGGTCCT+TGG + chr8.3:8397623-8397642 MS.gene58227:CDS 45.0%
!!! TCGTTTTGATGTCGGTGTCA+AGG + chr8.3:8396480-8396499 MS.gene58227:CDS 45.0%
!! TTTATATTATAAAAATGTAA+GGG - chr8.3:8397532-8397551 None:intergenic 5.0%
!!! TTTTATATTATAAAAATGTA+AGG - chr8.3:8397533-8397552 None:intergenic 5.0%
TAGACTTCTCCCTTCCAGAC+AGG + chr8.3:8396522-8396541 MS.gene58227:CDS 50.0%
!! GTCGGTGTCAAGGAGATTGA+AGG + chr8.3:8396490-8396509 MS.gene58227:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 8396094 8397658 8396094 ID=MS.gene58227
chr8.3 mRNA 8396094 8397658 8396094 ID=MS.gene58227.t1;Parent=MS.gene58227
chr8.3 exon 8396094 8396226 8396094 ID=MS.gene58227.t1.exon1;Parent=MS.gene58227.t1
chr8.3 CDS 8396094 8396226 8396094 ID=cds.MS.gene58227.t1;Parent=MS.gene58227.t1
chr8.3 exon 8396383 8396543 8396383 ID=MS.gene58227.t1.exon2;Parent=MS.gene58227.t1
chr8.3 CDS 8396383 8396543 8396383 ID=cds.MS.gene58227.t1;Parent=MS.gene58227.t1
chr8.3 exon 8397560 8397658 8397560 ID=MS.gene58227.t1.exon3;Parent=MS.gene58227.t1
chr8.3 CDS 8397560 8397658 8397560 ID=cds.MS.gene58227.t1;Parent=MS.gene58227.t1
Gene Sequence

>MS.gene58227

ATGGTGAGAGTCAGTGTTTTGAACGATGCTCTTAAGAGTATGTACAATGCTGAGAAAAGAGGAAAGCGTCAAGTTATGATTAGGCCTTCCTCTAAGGTTATCATCAAATTCCTCATCGTTATGCAAAAACACGGTATATATTCCTCTAAAATCTTTTTTTTTCCTTTCATTGAAGGTTGCATGATTCAATTATTTAGGGTTTTGATTTGATATAATTTTGTGGGTATGTTTGGTGGTTCAATTTTTTAGGGTTTTTGATTTGATATATTTTGTGGGTTATGCTTTATAGGTTATATTGGAGAGTTTGAGTATGTTGATGATCACAGATCTGGGAAAATCGTTGTTGAATTGAATGGTAGGTTGAACAAATGTGGGGTTATTAGTCCTCGTTTTGATGTCGGTGTCAAGGAGATTGAAGGTTGGACTGCTAGACTTCTCCCTTCCAGACAGGTCAGTTGAAAACAATACTAATTTTTGGGAACTTCTTTTGCATAGTGTCTGCTGGAATTGACTTATTTGAGCTTATCACCTGTGAGAATGTTTGGGAGAACTTATGAAAACGGCTTATGACATGTTCATAAGCTATTTTCAGCTTATTTTCATAAACATGGCTTATAGCTTATAAGATAACAATCTAAACTTAATTTATCTTTTGATAGGTGTTTATGTTATACTCCCTCTGTCTCACAATTATTGTCTCTTTAGCATTTTCTCTATTTCTCAAAATAATTGTCACTTTACAATACCAATGCAACATTTATTAGTTTTTTTCCACTATTATACCCTTATTTAATGAATTTCATCCAACTTAACTACTCCACCAACTACAATTAATAAGGGTGTTTTAGTAAATGCTACTAATTTTACCATTGAAATCAACACAATTAATCATTTCCTTAATAATCGTGCACAACCTTAAAACGACTAATATTGTGAGACGGAGGGAGTAAGCTGTAAAGTAAGCTGTTTATCCAAACAGGACCATGTTCTTACATATAGCTATGCTAGCATGTTCCACTTCCAATGCATATTTGTGTGAACTTTGAGAGCTTTCTTCAGTGTTATCGATTGCATGGTTACCATTTTGTTAAGCCGAGTAAATTTGTTCCAAATCATGATAGTTCATGCTTCATATTAAAGATTGGCTTGTTTCTTTTGTATAGTTTCTTACTCCTATTTGGTCTTTGTGGACAAAAAACACAGTTTAGTCCTAAAAAAAATCTCCGTTTTTTATGATCAATTTTAGCCTCTACTAGAGGCTTTTATGTGTGGAATTAGTAGTTTGAGCTCTGTCAACAATTTGTTGCTTGGATGATCTAGGTGATGATTTGTTCTTGTGCTGCTGTCCTTAAATTGTTATTATTTAAGATCAAATATTGAACTTATATGAACGTTGGGTCTTTCCTTTTGTGTGTAGTGTTGTTTCTTCTGTTTTCCCTTACATTTTTATAATATAAAATATGCAGTTTGGATACATTGTTTTGACCACTTCTGCTGGTATTATGGATCATGAAGAGGCTAGGAGAAAGAATGTTGGTGGTAAGGTCCTTGGCTTCTTCTACTAG

Protein sequence

>MS.gene58227.t1

MVRVSVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLIVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEGWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY