AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35135


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35135.t1 MTR_4g117470 100.000 130 0 0 1 130 1 130 1.05e-93 266
MS.gene35135.t1 MTR_4g094598 99.231 130 1 0 1 130 1 130 2.82e-93 265
MS.gene35135.t1 MTR_1g105415 99.231 130 1 0 1 130 1 130 2.82e-93 265
MS.gene35135.t1 MTR_8g101910 52.000 125 60 0 6 130 5 129 1.82e-45 144
MS.gene35135.t1 MTR_8g101910 49.474 95 48 0 6 100 5 99 1.72e-28 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35135.t1 AT5G59850 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene35135.t1 AT1G07770 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene35135.t1 AT1G07770 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene35135.t1 AT1G07770 99.231 130 1 0 1 130 1 130 1.76e-93 265
MS.gene35135.t1 AT3G46040 97.692 130 3 0 1 130 1 130 4.03e-92 262
MS.gene35135.t1 AT2G39590 91.538 130 11 0 1 130 1 130 4.30e-87 249
MS.gene35135.t1 AT4G29430 54.400 125 57 0 6 130 5 129 2.98e-47 149
MS.gene35135.t1 AT2G19720 53.600 125 58 0 6 130 5 129 3.11e-45 144

Find 28 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTGGTTATAGGATATAT+TGG 0.379495 4.4:-19543486 MS.gene35135:intron
GACTACCTCTGCTGGTATCA+TGG 0.414748 4.4:-19542019 MS.gene35135:CDS
ATTAGTCCTCGTTTCGATGT+TGG 0.460967 4.4:-19543384 MS.gene35135:CDS
AAAATTGTTGTGGAATTGAA+TGG 0.479932 4.4:-19543429 MS.gene35135:CDS
TACGTTGATGATCACAGGTC+TGG 0.486815 4.4:-19543453 MS.gene35135:CDS
AATGTTGGTGGTAAAGTGTT+GGG 0.506719 4.4:-19541973 MS.gene35135:CDS
AGGAAGGCCTAATCATAACT+TGG 0.515758 4.4:+19543636 None:intergenic
AGATTGAAGGATGGACTGCT+AGG 0.524175 4.4:-19543353 MS.gene35135:CDS
GGAAGCGCCAAGTTATGATT+AGG 0.530843 4.4:-19543643 MS.gene35135:CDS
ATTGTCTTGACTACCTCTGC+TGG 0.534592 4.4:-19542027 MS.gene35135:CDS
GAATGTTGGTGGTAAAGTGT+TGG 0.537027 4.4:-19541974 MS.gene35135:CDS
TCGAATACGTTGATGATCAC+AGG 0.543527 4.4:-19543458 MS.gene35135:CDS
GCTAGGAGAAAGAATGTTGG+TGG 0.567141 4.4:-19541985 MS.gene35135:CDS
TTGTAACAGCAAATATGGTG+AGG 0.575157 4.4:-19543718 None:intergenic
TAGGCTTCTTCCCTCAAGAC+AGG 0.576488 4.4:-19543334 MS.gene35135:intron
TGTAACAGCAAATATGGTGA+GGG 0.583163 4.4:-19543717 None:intergenic
GAGGCTAGGAGAAAGAATGT+TGG 0.584494 4.4:-19541988 MS.gene35135:CDS
TCATGGATCATGAAGAGGCT+AGG 0.585008 4.4:-19542002 MS.gene35135:CDS
ATGTACAATGCTGAGAAACG+TGG 0.619087 4.4:-19543665 MS.gene35135:CDS
GTTGGTGTCAAAGAGATTGA+AGG 0.620427 4.4:-19543366 MS.gene35135:CDS
CTTATCGTCATGCAAAAGCA+TGG 0.625917 4.4:-19543593 MS.gene35135:intron
CAGGTCTGGCAAAATTGTTG+TGG 0.629911 4.4:-19543439 MS.gene35135:CDS
AATGGAAGATTGAATAAGTG+TGG 0.630140 4.4:-19543411 MS.gene35135:CDS
TGGTATCATGGATCATGAAG+AGG 0.639197 4.4:-19542007 MS.gene35135:CDS
ATGATCCATGATACCAGCAG+AGG 0.644078 4.4:+19542014 None:intergenic
GTGTCAAAGAGATTGAAGGA+TGG 0.661917 4.4:-19543362 MS.gene35135:CDS
TGTACAATGCTGAGAAACGT+GGG 0.665211 4.4:-19543664 MS.gene35135:CDS
TTGACACCAACATCGAAACG+AGG 0.688564 4.4:+19543378 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAAAACTAATAGTTAA+AGG - chr4.4:19543421-19543440 MS.gene35135:CDS 15.0%
!! AAATTATAAAAACAGCTTAT+AGG - chr4.4:19542565-19542584 MS.gene35135:intron 15.0%
!! AAGAAAAACTAATAGTTAAA+GGG - chr4.4:19543422-19543441 MS.gene35135:CDS 15.0%
!! AAGCATAATAAGAAAATATA+CGG + chr4.4:19543121-19543140 None:intergenic 15.0%
!! ATAAAACACAAACTTAATTA+GGG + chr4.4:19542718-19542737 None:intergenic 15.0%
!! TATAAAACACAAACTTAATT+AGG + chr4.4:19542719-19542738 None:intergenic 15.0%
!!! ATTTTCTTATTATGCTTTTA+TGG - chr4.4:19543124-19543143 MS.gene35135:intron 15.0%
!!! CTAATTATGCTTTTATTTAT+TGG - chr4.4:19543200-19543219 MS.gene35135:intron 15.0%
!!! TTTTCTTATTATGCTTTTAT+GGG - chr4.4:19543125-19543144 MS.gene35135:intron 15.0%
!! AAAATATTAAAAGATGGGAT+GGG + chr4.4:19543286-19543305 None:intergenic 20.0%
!! AATTGAATAATTGTTGCAAA+AGG + chr4.4:19543334-19543353 None:intergenic 20.0%
!! ACAAAGTATATAAAGATTGT+CGG + chr4.4:19543459-19543478 None:intergenic 20.0%
!! AGAAAAACTAATAGTTAAAG+GGG - chr4.4:19543423-19543442 MS.gene35135:CDS 20.0%
!! CAAAAGCAAATAAATTAGAA+AGG + chr4.4:19542872-19542891 None:intergenic 20.0%
!! TAAAATATTAAAAGATGGGA+TGG + chr4.4:19543287-19543306 None:intergenic 20.0%
!! TAAATTCAAACCGTTATTAA+TGG - chr4.4:19543091-19543110 MS.gene35135:intron 20.0%
!!! CTTTCTAATTTATTTGCTTT+TGG - chr4.4:19542870-19542889 MS.gene35135:intron 20.0%
!!! TCATGAATCATTTTTAATGA+TGG - chr4.4:19543062-19543081 MS.gene35135:intron 20.0%
!!! TTTCTTATTATGCTTTTATG+GGG - chr4.4:19543126-19543145 MS.gene35135:intron 20.0%
! AAAATTGTTGTGGAATTGAA+TGG - chr4.4:19542235-19542254 MS.gene35135:intron 25.0%
! AGAAAACTAACTTATGTCAA+TGG + chr4.4:19542461-19542480 None:intergenic 25.0%
! GTATAGTAAATAGATAGAGT+GGG + chr4.4:19543176-19543195 None:intergenic 25.0%
! TATACTTTGTAGTAAATCGA+TGG - chr4.4:19543469-19543488 MS.gene35135:CDS 25.0%
! TCAAACTAGTATCATGTTTA+TGG + chr4.4:19542369-19542388 None:intergenic 25.0%
! TGATATATTGTATACTCTTG+TGG - chr4.4:19542162-19542181 MS.gene35135:intron 25.0%
! TTGACTTATGCTTCATATAT+TGG - chr4.4:19542989-19543008 MS.gene35135:intron 25.0%
!! AAATAAAAGCATAATTAGCG+AGG + chr4.4:19543198-19543217 None:intergenic 25.0%
!! GAACTTATGAAATTAGCTTA+TGG - chr4.4:19542494-19542513 MS.gene35135:intron 25.0%
!! GTATCATATGATTTTTCTTG+TGG - chr4.4:19543574-19543593 MS.gene35135:intron 25.0%
!!! ATTGACATAAGTTAGTTTTC+TGG - chr4.4:19542460-19542479 MS.gene35135:intron 25.0%
!!! GAATTTGATAATCACTTTTG+AGG + chr4.4:19542051-19542070 None:intergenic 25.0%
!!! TTGACATAAGTTAGTTTTCT+GGG - chr4.4:19542461-19542480 MS.gene35135:intron 25.0%
AAAATAAGCCGATAAAAGCT+TGG + chr4.4:19542528-19542547 None:intergenic 30.0%
AAACTTAATTAGGGTCTGTT+TGG + chr4.4:19542709-19542728 None:intergenic 30.0%
AAATAAGCCGATAAAAGCTT+GGG + chr4.4:19542527-19542546 None:intergenic 30.0%
AATGGAAGATTGAATAAGTG+TGG - chr4.4:19542253-19542272 MS.gene35135:intron 30.0%
ACAAGCAACATAACATTCAA+CGG + chr4.4:19543552-19543571 None:intergenic 30.0%
ATTTGAGCAACCAATAGTTA+GGG + chr4.4:19543601-19543620 None:intergenic 30.0%
CTTGTGGTTATAGGATATAT+TGG - chr4.4:19542178-19542197 MS.gene35135:intron 30.0%
GGTATAGTAAATAGATAGAG+TGG + chr4.4:19543177-19543196 None:intergenic 30.0%
TATAGTTCCTAACTTTCTTG+TGG - chr4.4:19542735-19542754 MS.gene35135:intron 30.0%
TGTTTGATCTCAACATAACA+AGG + chr4.4:19542839-19542858 None:intergenic 30.0%
TTCAAACCGTTATTAATGGT+TGG - chr4.4:19543095-19543114 MS.gene35135:intron 30.0%
TTGTATACTCTTGTGGTTAT+AGG - chr4.4:19542169-19542188 MS.gene35135:intron 30.0%
! CAATATTCCCAAGCTTTTAT+CGG - chr4.4:19542517-19542536 MS.gene35135:intron 30.0%
! TTATTATGCTTTTATGGGGT+GGG - chr4.4:19543130-19543149 MS.gene35135:intron 30.0%
!! ATGTAGTTGACTCTATTTTG+TGG - chr4.4:19542112-19542131 MS.gene35135:intron 30.0%
!! TGTAGTTGACTCTATTTTGT+GGG - chr4.4:19542113-19542132 MS.gene35135:intron 30.0%
!! TTGATAATCACTTTTGAGGA+AGG + chr4.4:19542047-19542066 None:intergenic 30.0%
!!! ATTTATTTGCTTTTGGTTCC+TGG - chr4.4:19542877-19542896 MS.gene35135:intron 30.0%
!!! CTAGTAGGCTTTTAAAACTT+GGG - chr4.4:19542920-19542939 MS.gene35135:intron 30.0%
!!! TCTAGTAGGCTTTTAAAACT+TGG - chr4.4:19542919-19542938 MS.gene35135:intron 30.0%
AAATCACTGTGACTCACTTA+CGG - chr4.4:19543012-19543031 MS.gene35135:intron 35.0%
ACAGAAACCTGTTCTATATG+GGG - chr4.4:19543380-19543399 MS.gene35135:CDS 35.0%
AGAATGACCACAAGAAAGTT+AGG + chr4.4:19542745-19542764 None:intergenic 35.0%
AGACAGAAACCTGTTCTATA+TGG - chr4.4:19543378-19543397 MS.gene35135:CDS 35.0%
ATACGGCCAACCATTAATAA+CGG + chr4.4:19543104-19543123 None:intergenic 35.0%
CCTACTAGACCTAACATAAA+GGG + chr4.4:19542908-19542927 None:intergenic 35.0%
CTACTAGACCTAACATAAAG+GGG + chr4.4:19542907-19542926 None:intergenic 35.0%
CTGAAGTTGCTATCATCATA+TGG - chr4.4:19543522-19543541 MS.gene35135:intron 35.0%
GACAGAAACCTGTTCTATAT+GGG - chr4.4:19543379-19543398 MS.gene35135:CDS 35.0%
GATTTGAGCAACCAATAGTT+AGG + chr4.4:19543602-19543621 None:intergenic 35.0%
TAGTAAATAGATAGAGTGGG+AGG + chr4.4:19543173-19543192 None:intergenic 35.0%
TTAATTAGGGTCTGTTTGGA+TGG + chr4.4:19542705-19542724 None:intergenic 35.0%
TTGCTCAAATCATGCAGTTT+GGG - chr4.4:19543611-19543630 MS.gene35135:CDS 35.0%
TTTGTGTACCACATTGACAT+TGG - chr4.4:19542765-19542784 MS.gene35135:intron 35.0%
! CTTATTATGCTTTTATGGGG+TGG - chr4.4:19543129-19543148 MS.gene35135:intron 35.0%
!! AATGTTGGTGGTAAAGTGTT+GGG - chr4.4:19543691-19543710 MS.gene35135:CDS 35.0%
!!! TAGGCTTTTAAAACTTGGGT+TGG - chr4.4:19542924-19542943 MS.gene35135:intron 35.0%
AATCACTGTGACTCACTTAC+GGG - chr4.4:19543013-19543032 MS.gene35135:intron 40.0%
AGGAAGGCCTAATCATAACT+TGG + chr4.4:19542031-19542050 None:intergenic 40.0%
ATGTACAATGCTGAGAAACG+TGG - chr4.4:19541999-19542018 MS.gene35135:CDS 40.0%
ATTAGTCCTCGTTTCGATGT+TGG - chr4.4:19542280-19542299 MS.gene35135:intron 40.0%
CCCTTTATGTTAGGTCTAGT+AGG - chr4.4:19542905-19542924 MS.gene35135:intron 40.0%
CTAACATAAAGGGGAATTCC+AGG + chr4.4:19542898-19542917 None:intergenic 40.0%
CTGGAATTCCCCTTTATGTT+AGG - chr4.4:19542896-19542915 MS.gene35135:intron 40.0%
CTGTATTCCCCATATAGAAC+AGG + chr4.4:19543390-19543409 None:intergenic 40.0%
CTTATCGTCATGCAAAAGCA+TGG - chr4.4:19542071-19542090 MS.gene35135:intron 40.0%
GCCTACTAGACCTAACATAA+AGG + chr4.4:19542909-19542928 None:intergenic 40.0%
GGTGGGAGTAGAATATTCAT+TGG - chr4.4:19543147-19543166 MS.gene35135:intron 40.0%
GTGTCAAAGAGATTGAAGGA+TGG - chr4.4:19542302-19542321 MS.gene35135:intron 40.0%
GTTGCTCAAATCATGCAGTT+TGG - chr4.4:19543610-19543629 MS.gene35135:CDS 40.0%
TAAGTCAAGACAAACACTGC+CGG + chr4.4:19542977-19542996 None:intergenic 40.0%
TCAAGACTCCAATGTCAATG+TGG + chr4.4:19542776-19542795 None:intergenic 40.0%
TGGTATCATGGATCATGAAG+AGG - chr4.4:19543657-19543676 MS.gene35135:CDS 40.0%
TGTACAATGCTGAGAAACGT+GGG - chr4.4:19542000-19542019 MS.gene35135:CDS 40.0%
TTCTTGTGGTCCCTAACTAT+TGG - chr4.4:19543588-19543607 MS.gene35135:intron 40.0%
! CATGCTTTTGCATGACGATA+AGG + chr4.4:19542073-19542092 None:intergenic 40.0%
! TCATGCAAAAGCATGGTATG+TGG - chr4.4:19542078-19542097 MS.gene35135:intron 40.0%
!! AACTTGGGTTGGAATTGTAG+AGG - chr4.4:19542935-19542954 MS.gene35135:intron 40.0%
!! ACTTGGGTTGGAATTGTAGA+GGG - chr4.4:19542936-19542955 MS.gene35135:intron 40.0%
!! GAATGTTGGTGGTAAAGTGT+TGG - chr4.4:19543690-19543709 MS.gene35135:CDS 40.0%
!! GTTGGTGTCAAAGAGATTGA+AGG - chr4.4:19542298-19542317 MS.gene35135:intron 40.0%
!! TCGAATACGTTGATGATCAC+AGG - chr4.4:19542206-19542225 MS.gene35135:intron 40.0%
!! TTTTAGCTGACCTGTCTTGA+GGG + chr4.4:19542343-19542362 None:intergenic 40.0%
!!! TTTTTAGCTGACCTGTCTTG+AGG + chr4.4:19542344-19542363 None:intergenic 40.0%
AGGGGTTAACCAGAACAATC+CGG - chr4.4:19542955-19542974 MS.gene35135:intron 45.0%
ATGATCCATGATACCAGCAG+AGG + chr4.4:19543653-19543672 None:intergenic 45.0%
ATTGTCTTGACTACCTCTGC+TGG - chr4.4:19543637-19543656 MS.gene35135:CDS 45.0%
CAGGTCTGGCAAAATTGTTG+TGG - chr4.4:19542225-19542244 MS.gene35135:intron 45.0%
GACTCACTTACGGGACTATT+TGG - chr4.4:19543022-19543041 MS.gene35135:intron 45.0%
GAGGCTAGGAGAAAGAATGT+TGG - chr4.4:19543676-19543695 MS.gene35135:CDS 45.0%
GCTAGGAGAAAGAATGTTGG+TGG - chr4.4:19543679-19543698 MS.gene35135:CDS 45.0%
TCATGGATCATGAAGAGGCT+AGG - chr4.4:19543662-19543681 MS.gene35135:CDS 45.0%
TTGACACCAACATCGAAACG+AGG + chr4.4:19542289-19542308 None:intergenic 45.0%
! GCAAAAGCATGGTATGTGGT+TGG - chr4.4:19542082-19542101 MS.gene35135:intron 45.0%
!! AGATTGAAGGATGGACTGCT+AGG - chr4.4:19542311-19542330 MS.gene35135:intron 45.0%
!! CTTGGGTTGGAATTGTAGAG+GGG - chr4.4:19542937-19542956 MS.gene35135:intron 45.0%
!! GGAAGCGCCAAGTTATGATT+AGG - chr4.4:19542021-19542040 MS.gene35135:CDS 45.0%
!! TACGTTGATGATCACAGGTC+TGG - chr4.4:19542211-19542230 MS.gene35135:intron 45.0%
!! AAATTAAAATATTAAAAGAT+GGG + chr4.4:19543291-19543310 None:intergenic 5.0%
!! TAAATTAAAATATTAAAAGA+TGG + chr4.4:19543292-19543311 None:intergenic 5.0%
CAAACACTGCCGGATTGTTC+TGG + chr4.4:19542967-19542986 None:intergenic 50.0%
GACTACCTCTGCTGGTATCA+TGG - chr4.4:19543645-19543664 MS.gene35135:CDS 50.0%
TAGGCTTCTTCCCTCAAGAC+AGG - chr4.4:19542330-19542349 MS.gene35135:intron 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 19541960 19543726 19541960 ID=MS.gene35135
chr4.4 mRNA 19541960 19543726 19541960 ID=MS.gene35135.t1;Parent=MS.gene35135
chr4.4 exon 19543594 19543726 19543594 ID=MS.gene35135.t1.exon1;Parent=MS.gene35135.t1
chr4.4 CDS 19543594 19543726 19543594 ID=cds.MS.gene35135.t1;Parent=MS.gene35135.t1
chr4.4 exon 19543335 19543495 19543335 ID=MS.gene35135.t1.exon2;Parent=MS.gene35135.t1
chr4.4 CDS 19543335 19543495 19543335 ID=cds.MS.gene35135.t1;Parent=MS.gene35135.t1
chr4.4 exon 19541960 19542058 19541960 ID=MS.gene35135.t1.exon3;Parent=MS.gene35135.t1
chr4.4 CDS 19541960 19542058 19541960 ID=cds.MS.gene35135.t1;Parent=MS.gene35135.t1
Gene Sequence

>MS.gene35135

ATGGTGAGGGTTAGTGTTTTGAACGATGCACTGAAGAGCATGTACAATGCTGAGAAACGTGGGAAGCGCCAAGTTATGATTAGGCCTTCCTCAAAAGTGATTATCAAATTCCTTATCGTCATGCAAAAGCATGGTATGTGGTTGGTTGATTCATGTAGTTGACTCTATTTTGTGGGATAATGATTTTTTTTTTTTTTTTATGTGATATATTGTATACTCTTGTGGTTATAGGATATATTGGAGAGTTCGAATACGTTGATGATCACAGGTCTGGCAAAATTGTTGTGGAATTGAATGGAAGATTGAATAAGTGTGGTGTTATTAGTCCTCGTTTCGATGTTGGTGTCAAAGAGATTGAAGGATGGACTGCTAGGCTTCTTCCCTCAAGACAGGTCAGCTAAAAAGACCATAAACATGATACTAGTTTGATAGTTTAATTTACATGATTGTTTTTACTGCACAATATGTGACTAATTTGATTGACTTATTTGAGCTTATCCATTGACATAAGTTAGTTTTCTGGGACTGTATTGTGAACTTATGAAATTAGCTTATGGCAATATTCCCAAGCTTTTATCGGCTTATTTTCATATGCTCTTCAGTATAAATTATAAAAACAGCTTATAGGATATACAAAAACAATCTAACTTTTTATTTTATCTTTTTCTATAAAAATAAGCTTATGCATAAGAGCTTCTTGTGATAAGATAAGTGCTTGTGCTATAAGCTGTGAATAAACAGTCCATCCAAACAGACCCTAATTAAGTTTGTGTTTTATAGTTCCTAACTTTCTTGTGGTCATTCTTTTGTGTACCACATTGACATTGGAGTCTTGATCTCAAGTATGAGCTTTAGTTCATTGTTTGTTTGTTGTATCCTTGTTATGTTGAGATCAAACAATTTTTTTTTCCTTTCTAATTTATTTGCTTTTGGTTCCTGGAATTCCCCTTTATGTTAGGTCTAGTAGGCTTTTAAAACTTGGGTTGGAATTGTAGAGGGGTTAACCAGAACAATCCGGCAGTGTTTGTCTTGACTTATGCTTCATATATTGGAAATCACTGTGACTCACTTACGGGACTATTTGGATAAAATCAAATCATGATCATGAATCATTTTTAATGATGGAGCATTTAAATTCAAACCGTTATTAATGGTTGGCCGTATATTTTCTTATTATGCTTTTATGGGGTGGGAGTAGAATATTCATTGGCCTCCCACTCTATCTATTTACTATACCTCGCTAATTATGCTTTTATTTATTGGTCTTTTCATTTTTGTACAAACAACTTAAAAACACGACTACTTATTTTACAATATGTGCTACCCATCCCATCTTTTAATATTTTAATTTATATTCATAATTATATCTTGCCTTTTGCAACAATTATTCAATTGTAATGTTTATATACTTGACATTTAGACAGAAACCTGTTCTATATGGGGAATACAGATTTTACATTTAAAGAAAAACTAATAGTTAAAGGGGCAATTATATACCGACAATCTTTATATACTTTGTAGTAAATCGATGGTTTATTTTACATGTAGATGCAAACTGAACTCTGAAGTTGCTATCATCATATGGCAAACCGTTGAATGTTATGTTGCTTGTTTGTATCATATGATTTTTCTTGTGGTCCCTAACTATTGGTTGCTCAAATCATGCAGTTTGGGTATATTGTCTTGACTACCTCTGCTGGTATCATGGATCATGAAGAGGCTAGGAGAAAGAATGTTGGTGGTAAAGTGTTGGGTTTTTTCTATTAG

Protein sequence

>MS.gene35135.t1

MVRVSVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLIVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEGWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY