AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044081

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044081.t1 MTR_1g018760 100.000 325 0 0 1 325 1 325 0.0 662
MS.gene044081.t1 MTR_3g102370 85.846 325 43 2 1 325 1 322 0.0 570
MS.gene044081.t1 MTR_1g094030 83.385 325 49 1 1 325 1 320 0.0 552
MS.gene044081.t1 MTR_4g109900 59.006 322 127 1 3 324 2 318 2.83e-132 379
MS.gene044081.t1 MTR_4g109920 59.752 323 124 2 3 324 2 319 3.34e-132 379
MS.gene044081.t1 MTR_8g031580 30.449 312 165 11 6 281 23 318 5.62e-23 98.2
MS.gene044081.t1 MTR_8g031580 30.449 312 165 11 6 281 23 318 1.38e-22 97.4
MS.gene044081.t1 MTR_3g008180 28.754 313 182 13 38 316 40 345 2.07e-18 85.1
MS.gene044081.t1 MTR_3g008180 28.226 248 149 10 93 316 4 246 5.10e-14 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044081.t1 AT3G16150 84.615 325 50 0 1 325 1 325 0.0 566
MS.gene044081.t1 AT5G08100 57.492 327 121 4 1 324 1 312 3.01e-125 361
MS.gene044081.t1 AT5G08100 57.490 247 88 3 80 324 1 232 3.77e-94 279
MS.gene044081.t1 AT4G00590 27.187 320 179 8 6 285 15 320 1.65e-20 91.7

Find 94 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACGATGCGGTGCCGTTTC+AGG 0.158496 1.2:+4631572 MS.gene044081:CDS
GGTTCATGGAGGTTGGAATT+TGG 0.195033 1.2:+4632919 MS.gene044081:CDS
ACTACGCACCCGACTGTTTC+AGG 0.214500 1.2:-4632546 None:intergenic
GGATCTGCCCTGACGGAAAA+AGG 0.260928 1.2:+4631509 MS.gene044081:CDS
GTTCATGGAGGTTGGAATTT+GGG 0.262833 1.2:+4632920 MS.gene044081:CDS
ACTAAACGAGCCAGTGAAAT+TGG 0.271206 1.2:-4631614 None:intergenic
TTAACTGCAATGGCATGTTT+AGG 0.291387 1.2:+4632877 MS.gene044081:CDS
TTTGTAGGGTGTGGAGGTTC+TGG 0.300802 1.2:+4631936 MS.gene044081:intron
AACGGATCCATTATTCAATT+CGG 0.328084 1.2:+4631481 MS.gene044081:CDS
AGTAAAGGAGAAGTTGCTTA+TGG 0.336064 1.2:+4632852 MS.gene044081:CDS
TAACTGCAATGGCATGTTTA+GGG 0.343986 1.2:+4632878 MS.gene044081:CDS
GTTATTGAAGGTGAGGGAAT+TGG 0.347604 1.2:+4631457 MS.gene044081:intron
CTTACTCGTGTTCTCAATCT+TGG 0.354252 1.2:+4631119 MS.gene044081:CDS
TGTGCTGCTGCCACGTCAAC+AGG 0.356128 1.2:+4632588 MS.gene044081:CDS
TGACGTTGAAGAGGGAGATT+AGG 0.362167 1.2:-4631077 None:intergenic
GTGTGCTGCAGTTTGATTAT+CGG 0.363877 1.2:+4632442 MS.gene044081:intron
AACACGAGTAAGAAGTTGTT+TGG 0.379765 1.2:-4631108 None:intergenic
AACATCGATGGCAGAAAGAT+TGG 0.396249 1.2:-4631159 None:intergenic
ATTGCTGTGCATGGTGGTGC+TGG 0.397163 1.2:+4631047 MS.gene044081:CDS
AGGGGATGTGCTTCTGAGGA+TGG 0.401581 1.2:+4632897 MS.gene044081:CDS
ACGGATCCATTATTCAATTC+GGG 0.402987 1.2:+4631482 MS.gene044081:CDS
TGCTTCTGAGGATGGGTTCA+TGG 0.405891 1.2:+4632905 MS.gene044081:CDS
CCACGACCCGAATTGAATAA+TGG 0.409899 1.2:-4631488 None:intergenic
CTCATTGCTGTTTCTAGTAA+AGG 0.414337 1.2:+4632837 MS.gene044081:CDS
TACTGTGGTTAAACCTGAAA+CGG 0.417200 1.2:-4631585 None:intergenic
TTTGATTATCGGATTCCATC+TGG 0.417677 1.2:+4632453 MS.gene044081:CDS
ATTGGTGACTCACCTTTGAT+AGG 0.420480 1.2:+4632639 MS.gene044081:CDS
AGAGAGAAACATGGGTGGTT+GGG 0.423456 1.2:+4631022 None:intergenic
CCATTATTCAATTCGGGTCG+TGG 0.423817 1.2:+4631488 MS.gene044081:CDS
GGTTGGGCAATTGCTGTGCA+TGG 0.428468 1.2:+4631038 MS.gene044081:CDS
CACTTTGGCGCGAGAGGTGT+CGG 0.430026 1.2:+4632731 MS.gene044081:CDS
GGAAATGGAGGCTAGCATAA+TGG 0.434511 1.2:+4631538 MS.gene044081:CDS
GACGTTGAAGAGGGAGATTA+GGG 0.438575 1.2:-4631076 None:intergenic
ATTAAGAATCGTTTGGATGA+AGG 0.439748 1.2:+4632804 MS.gene044081:CDS
AAAGGAAGCAAATACAATCT+TGG 0.457652 1.2:+4632008 MS.gene044081:CDS
GGGGATGTGCTTCTGAGGAT+GGG 0.460240 1.2:+4632898 MS.gene044081:CDS
CAGCAGCACACCTTCCTTCA+CGG 0.468263 1.2:-4632575 None:intergenic
TCAGGTGCGTAGACACTCAT+TGG 0.468761 1.2:-4632528 None:intergenic
GACTCACCTTTGATAGGAGC+CGG 0.472240 1.2:+4632645 MS.gene044081:CDS
ACCTACGCGTGTAACCTTGC+TGG 0.480120 1.2:+4632669 MS.gene044081:CDS
GAGGATGGGTTCATGGAGGT+TGG 0.480884 1.2:+4632912 MS.gene044081:CDS
AGCGCCGTTAAAAGCAAGAT+AGG 0.481695 1.2:-4631657 None:intergenic
GCTTATGGATTTAACTGCAA+TGG 0.484263 1.2:+4632867 MS.gene044081:CDS
TTCACTGGCTCGTTTAGTCA+TGG 0.486469 1.2:+4631619 MS.gene044081:CDS
AAGGTTACACGCGTAGGTCC+CGG 0.486913 1.2:-4632664 None:intergenic
GAAAGTCCAATGAAAATGAA+TGG 0.491181 1.2:+4632501 MS.gene044081:CDS
AACAGTCGGGTGCGTAGTTG+TGG 0.493878 1.2:+4632551 MS.gene044081:CDS
TATGATAGCCTCACCTTCAC+CGG 0.497356 1.2:-4632703 None:intergenic
CTTCTTCTTGACGTTGAAGA+GGG 0.497744 1.2:-4631085 None:intergenic
CGTTTGGATGAAGGATATGC+TGG 0.504298 1.2:+4632813 MS.gene044081:CDS
GTGATGGAATACAAAGGTCT+TGG 0.504451 1.2:+4632756 MS.gene044081:CDS
AACAAAATGATTGGTCGGAT+TGG 0.504757 1.2:+4632621 MS.gene044081:CDS
GAGAGAGAAACATGGGTGGT+TGG 0.505927 1.2:+4631021 None:intergenic
GGGCTAATGAACAAAATGAT+TGG 0.512413 1.2:+4632612 MS.gene044081:CDS
GCTTCTTCTTGACGTTGAAG+AGG 0.520285 1.2:-4631086 None:intergenic
GCGAGAGGTGTCGGCTGTGA+TGG 0.520900 1.2:+4632740 MS.gene044081:CDS
GGCGTCTCATGCACCGGTGA+AGG 0.524434 1.2:+4632690 MS.gene044081:CDS
GGCTATCATACGTGGCACTT+TGG 0.535143 1.2:+4632716 MS.gene044081:CDS
GCTGCTGCCACGTCAACAGG+CGG 0.537860 1.2:+4632591 MS.gene044081:CDS
CTTGCTGGCGTCTCATGCAC+CGG 0.541455 1.2:+4632684 MS.gene044081:CDS
CATTAGCCCGCCTGTTGACG+TGG 0.549451 1.2:-4632598 None:intergenic
CTGCTGCCACGTCAACAGGC+GGG 0.561806 1.2:+4632592 MS.gene044081:CDS
TAATGAACAAAATGATTGGT+CGG 0.563124 1.2:+4632616 MS.gene044081:CDS
GAAGGTGAGGGAATTGGAAA+CGG 0.565330 1.2:+4631463 MS.gene044081:intron
CAATGTTGGTATGCTTAAGC+TGG 0.567336 1.2:+4631984 MS.gene044081:CDS
GGGTCGTGGATCTGCCCTGA+CGG 0.570628 1.2:+4631502 MS.gene044081:CDS
GTTTAGGGGATGTGCTTCTG+AGG 0.570942 1.2:+4632893 MS.gene044081:CDS
ACGTGGCACTTTGGCGCGAG+AGG 0.574745 1.2:+4632725 MS.gene044081:CDS
TAGGTCCCGGCTCCTATCAA+AGG 0.574816 1.2:-4632651 None:intergenic
TTTATCACCGCTGACAATGT+TGG 0.576194 1.2:+4631970 MS.gene044081:CDS
TCTACGCACCTGAAACAGTC+GGG 0.579137 1.2:+4632538 MS.gene044081:CDS
CCACATGTTTCGTATCCAGA+TGG 0.581145 1.2:-4632468 None:intergenic
AACTGCAATGGCATGTTTAG+GGG 0.582673 1.2:+4632879 MS.gene044081:CDS
CATCGATGTTGTTGAACTTG+TGG 0.587378 1.2:+4631172 MS.gene044081:CDS
CTCATGCACCGGTGAAGGTG+AGG 0.595337 1.2:+4632695 MS.gene044081:CDS
TCTTGGTCTTCAAGAAGCAG+TGG 0.595757 1.2:+4632773 MS.gene044081:CDS
ATGGAGGCTAGCATAATGGA+TGG 0.598729 1.2:+4631542 MS.gene044081:CDS
ACTCACCTTTGATAGGAGCC+GGG 0.601444 1.2:+4632646 MS.gene044081:CDS
TGCGTAGTTGTGGACCGTGA+AGG 0.611085 1.2:+4632561 MS.gene044081:CDS
GTCTACGCACCTGAAACAGT+CGG 0.614969 1.2:+4632537 MS.gene044081:CDS
CACAAGTTCAACAACATCGA+TGG 0.615685 1.2:-4631171 None:intergenic
TTGAGAGAGAGAAACATGGG+TGG 0.619739 1.2:+4631017 None:intergenic
AAAAGGAACGGTGGAAATGG+AGG 0.622339 1.2:+4631526 MS.gene044081:CDS
TGGTATGCTTAAGCTGGCAA+AGG 0.632666 1.2:+4631990 MS.gene044081:CDS
TTAAAAGCAAGATAGGAATG+AGG 0.636686 1.2:-4631650 None:intergenic
CGGTGCATGAGACGCCAGCA+AGG 0.648195 1.2:-4632683 None:intergenic
TGGGCAATTGCTGTGCATGG+TGG 0.661106 1.2:+4631041 MS.gene044081:CDS
TAGTTGTGGACCGTGAAGGA+AGG 0.661356 1.2:+4632565 MS.gene044081:CDS
CCATCTGGATACGAAACATG+TGG 0.678131 1.2:+4632468 MS.gene044081:CDS
GCCAGCAAGGTTACACGCGT+AGG 0.682958 1.2:-4632670 None:intergenic
GAAGGTGAGGCTATCATACG+TGG 0.685255 1.2:+4632708 MS.gene044081:CDS
AAGCATACCAACATTGTCAG+CGG 0.698333 1.2:-4631977 None:intergenic
TTCTGAGGATGGGTTCATGG+AGG 0.702330 1.2:+4632908 MS.gene044081:CDS
TCGGCTGTGATGGAATACAA+AGG 0.710713 1.2:+4632750 MS.gene044081:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTTTATTTACAAAAAAAT+AGG + chr1.2:4631798-4631817 MS.gene044081:intron 10.0%
!!! GAAAAGATATTTTATTTTTT+GGG + chr1.2:4631820-4631839 MS.gene044081:intron 10.0%
!!! TTATTTATTTTTGTTATTGA+AGG + chr1.2:4631445-4631464 MS.gene044081:intron 10.0%
!! TGAATTATTATTACATTGTA+CGG + chr1.2:4632064-4632083 MS.gene044081:intron 15.0%
!!! AATATATTTTAACTATTCCA+AGG + chr1.2:4631396-4631415 MS.gene044081:intron 15.0%
!!! ATTATTTTACTTGTTTTTGT+AGG + chr1.2:4631921-4631940 MS.gene044081:intron 15.0%
!!! ATTCTTTCATTCATTTTTTA+CGG + chr1.2:4632355-4632374 MS.gene044081:intron 15.0%
!!! GGAAAAGATATTTTATTTTT+TGG + chr1.2:4631819-4631838 MS.gene044081:intron 15.0%
!!! GTAAAAATTATTTTCAGATT+GGG + chr1.2:4632153-4632172 MS.gene044081:intron 15.0%
!!! TTATTTTACTTGTTTTTGTA+GGG + chr1.2:4631922-4631941 MS.gene044081:intron 15.0%
!! AAAAAAAAATTGCAGAGATT+TGG - chr1.2:4631767-4631786 None:intergenic 20.0%
!! ATTATTATCAAATTCAACCA+TGG + chr1.2:4632037-4632056 MS.gene044081:intron 20.0%
!! GAATTATTATTACATTGTAC+GGG + chr1.2:4632065-4632084 MS.gene044081:intron 20.0%
!! TAATAATAATTCAAGATCCA+TGG - chr1.2:4632057-4632076 None:intergenic 20.0%
!! TGTTTATAAACAAAAGAGTT+TGG - chr1.2:4632269-4632288 None:intergenic 20.0%
!!! AGATATTTTATTTTTTGGGT+TGG + chr1.2:4631824-4631843 MS.gene044081:intron 20.0%
!!! GGTAAAAATTATTTTCAGAT+TGG + chr1.2:4632152-4632171 MS.gene044081:intron 20.0%
! AGTAAAAAATCCAATTTCAC+TGG + chr1.2:4631604-4631623 MS.gene044081:CDS 25.0%
! TAATGAACAAAATGATTGGT+CGG + chr1.2:4632616-4632635 MS.gene044081:CDS 25.0%
! TATTGTATTCATGCATAGTT+TGG + chr1.2:4632198-4632217 MS.gene044081:intron 25.0%
! TCACATATTGAAATAAACGT+GGG + chr1.2:4632131-4632150 MS.gene044081:intron 25.0%
! TTCACATATTGAAATAAACG+TGG + chr1.2:4632130-4632149 MS.gene044081:intron 25.0%
! TTCTTGTTAATCATGTAGTT+TGG - chr1.2:4631378-4631397 None:intergenic 25.0%
! TTTAACTATTCCAAGGAATT+TGG + chr1.2:4631403-4631422 MS.gene044081:intron 25.0%
!! TAAAGAGAAAGTGTACTAAT+TGG - chr1.2:4632225-4632244 None:intergenic 25.0%
!! TTTTGTCATTAAGAATCGTT+TGG + chr1.2:4632797-4632816 MS.gene044081:CDS 25.0%
!!! ATTTTTGTTATTGAAGGTGA+GGG + chr1.2:4631451-4631470 MS.gene044081:intron 25.0%
!!! TATTTTTGTTATTGAAGGTG+AGG + chr1.2:4631450-4631469 MS.gene044081:intron 25.0%
!!! TGAAATTGGATTTTTTACTG+TGG - chr1.2:4631603-4631622 None:intergenic 25.0%
!!! TTTTTGGTTAACGTTGTTTA+AGG - chr1.2:4631742-4631761 None:intergenic 25.0%
AAAGGAAGCAAATACAATCT+TGG + chr1.2:4632008-4632027 MS.gene044081:CDS 30.0%
AACAAACACTTTGTCTTCAT+TGG - chr1.2:4632304-4632323 None:intergenic 30.0%
AACGGATCCATTATTCAATT+CGG + chr1.2:4631481-4631500 MS.gene044081:CDS 30.0%
ATTAAGAATCGTTTGGATGA+AGG + chr1.2:4632804-4632823 MS.gene044081:CDS 30.0%
CAATGCATGTATTAGATTCA+CGG - chr1.2:4631345-4631364 None:intergenic 30.0%
CCATGAAGTAATAATGGTAA+CGG - chr1.2:4631306-4631325 None:intergenic 30.0%
GAAAGTCCAATGAAAATGAA+TGG + chr1.2:4632501-4632520 MS.gene044081:CDS 30.0%
TTAAAAGCAAGATAGGAATG+AGG - chr1.2:4631653-4631672 None:intergenic 30.0%
! AGATTCACGGTTATTTGTTA+TGG - chr1.2:4631332-4631351 None:intergenic 30.0%
! ATTACATTGTACGGGTTAAA+AGG + chr1.2:4632073-4632092 MS.gene044081:intron 30.0%
! TTACATTGTACGGGTTAAAA+GGG + chr1.2:4632074-4632093 MS.gene044081:intron 30.0%
!! AGTGTGTGTTTTTCTGAAAA+GGG + chr1.2:4632103-4632122 MS.gene044081:intron 30.0%
!! CATTCCTATCTTGCTTTTAA+CGG + chr1.2:4631653-4631672 MS.gene044081:CDS 30.0%
!!! TTGCAGAGATTTGGTTTTTT+TGG - chr1.2:4631758-4631777 None:intergenic 30.0%
!!! TTTATTTTTTGGGTTGGTGT+TGG + chr1.2:4631830-4631849 MS.gene044081:intron 30.0%
AAAAAAGGGACATGACAAGA+TGG - chr1.2:4631271-4631290 None:intergenic 35.0%
AACACGAGTAAGAAGTTGTT+TGG - chr1.2:4631111-4631130 None:intergenic 35.0%
AACTATTCCAAGGAATTTGG+AGG + chr1.2:4631406-4631425 MS.gene044081:intron 35.0%
ACGGATCCATTATTCAATTC+GGG + chr1.2:4631482-4631501 MS.gene044081:CDS 35.0%
ACTATTCCAAGGAATTTGGA+GGG + chr1.2:4631407-4631426 MS.gene044081:intron 35.0%
AGTAAAGGAGAAGTTGCTTA+TGG + chr1.2:4632852-4632871 MS.gene044081:CDS 35.0%
CCGTTACCATTATTACTTCA+TGG + chr1.2:4631303-4631322 MS.gene044081:intron 35.0%
GCTTATGGATTTAACTGCAA+TGG + chr1.2:4632867-4632886 MS.gene044081:CDS 35.0%
GGGCTAATGAACAAAATGAT+TGG + chr1.2:4632612-4632631 MS.gene044081:CDS 35.0%
GGTGGAAAAGGAACAAAAAA+GGG - chr1.2:4631285-4631304 None:intergenic 35.0%
TAACTGCAATGGCATGTTTA+GGG + chr1.2:4632878-4632897 MS.gene044081:CDS 35.0%
TACTGTGGTTAAACCTGAAA+CGG - chr1.2:4631588-4631607 None:intergenic 35.0%
TGGTAGCCATGAAGTAATAA+TGG - chr1.2:4631312-4631331 None:intergenic 35.0%
TTAACTGCAATGGCATGTTT+AGG + chr1.2:4632877-4632896 MS.gene044081:CDS 35.0%
TTTCAGAAAAACACACACTC+TGG - chr1.2:4632102-4632121 None:intergenic 35.0%
TTTGATTATCGGATTCCATC+TGG + chr1.2:4632453-4632472 MS.gene044081:CDS 35.0%
! AACAAAATGATTGGTCGGAT+TGG + chr1.2:4632621-4632640 MS.gene044081:CDS 35.0%
! AGGAAGTGTGGACAATATTA+TGG - chr1.2:4631227-4631246 None:intergenic 35.0%
! GGAAGTCCATTCATTTTCAT+TGG - chr1.2:4632510-4632529 None:intergenic 35.0%
! TACATTGTACGGGTTAAAAG+GGG + chr1.2:4632075-4632094 MS.gene044081:intron 35.0%
! TGAAGATTTTGCTAGACAAC+AGG + chr1.2:4631679-4631698 MS.gene044081:CDS 35.0%
!! AATAATGGTAACGGTGGAAA+AGG - chr1.2:4631297-4631316 None:intergenic 35.0%
!! CTCATTGCTGTTTCTAGTAA+AGG + chr1.2:4632837-4632856 MS.gene044081:CDS 35.0%
!! GAGTGTGTGTTTTTCTGAAA+AGG + chr1.2:4632102-4632121 MS.gene044081:intron 35.0%
!! TGAAGTAATAATGGTAACGG+TGG - chr1.2:4631303-4631322 None:intergenic 35.0%
!!! TTACTTGTTTTTGTAGGGTG+TGG + chr1.2:4631927-4631946 MS.gene044081:intron 35.0%
AACATCGATGGCAGAAAGAT+TGG - chr1.2:4631162-4631181 None:intergenic 40.0%
AACTGCAATGGCATGTTTAG+GGG + chr1.2:4632879-4632898 MS.gene044081:CDS 40.0%
AAGATGGAAACATGTGTGTG+TGG - chr1.2:4631255-4631274 None:intergenic 40.0%
AAGCATACCAACATTGTCAG+CGG - chr1.2:4631980-4631999 None:intergenic 40.0%
ACTAAACGAGCCAGTGAAAT+TGG - chr1.2:4631617-4631636 None:intergenic 40.0%
AGATGGAAACATGTGTGTGT+GGG - chr1.2:4631254-4631273 None:intergenic 40.0%
ATAATCCCCTCCAAATTCCT+TGG - chr1.2:4631416-4631435 None:intergenic 40.0%
CAATGTTGGTATGCTTAAGC+TGG + chr1.2:4631984-4632003 MS.gene044081:CDS 40.0%
CACAAGTTCAACAACATCGA+TGG - chr1.2:4631174-4631193 None:intergenic 40.0%
CATCGATGTTGTTGAACTTG+TGG + chr1.2:4631172-4631191 MS.gene044081:CDS 40.0%
CGGTGGAAAAGGAACAAAAA+AGG - chr1.2:4631286-4631305 None:intergenic 40.0%
CTATTCCAAGGAATTTGGAG+GGG + chr1.2:4631408-4631427 MS.gene044081:intron 40.0%
CTTACTCGTGTTCTCAATCT+TGG + chr1.2:4631119-4631138 MS.gene044081:CDS 40.0%
CTTCTTCTTGACGTTGAAGA+GGG - chr1.2:4631088-4631107 None:intergenic 40.0%
GTGTGCTGCAGTTTGATTAT+CGG + chr1.2:4632442-4632461 MS.gene044081:intron 40.0%
GTTATTGAAGGTGAGGGAAT+TGG + chr1.2:4631457-4631476 MS.gene044081:intron 40.0%
GTTCATGGAGGTTGGAATTT+GGG + chr1.2:4632920-4632939 MS.gene044081:CDS 40.0%
TTTAAGGAAGACACACACGT+GGG - chr1.2:4631726-4631745 None:intergenic 40.0%
TTTATCACCGCTGACAATGT+TGG + chr1.2:4631970-4631989 MS.gene044081:CDS 40.0%
! ATTGGTGACTCACCTTTGAT+AGG + chr1.2:4632639-4632658 MS.gene044081:CDS 40.0%
! GTGATGGAATACAAAGGTCT+TGG + chr1.2:4632756-4632775 MS.gene044081:CDS 40.0%
!! TTGGTGTTGGAACTTTGCTA+AGG + chr1.2:4631843-4631862 MS.gene044081:intron 40.0%
AAAAGGAACGGTGGAAATGG+AGG + chr1.2:4631526-4631545 MS.gene044081:CDS 45.0%
AGCGCCGTTAAAAGCAAGAT+AGG - chr1.2:4631660-4631679 None:intergenic 45.0%
CCACATGTTTCGTATCCAGA+TGG - chr1.2:4632471-4632490 None:intergenic 45.0%
CCACGACCCGAATTGAATAA+TGG - chr1.2:4631491-4631510 None:intergenic 45.0%
CCATCTGGATACGAAACATG+TGG + chr1.2:4632468-4632487 MS.gene044081:CDS 45.0%
CCATTATTCAATTCGGGTCG+TGG + chr1.2:4631488-4631507 MS.gene044081:CDS 45.0%
CGTTTGGATGAAGGATATGC+TGG + chr1.2:4632813-4632832 MS.gene044081:CDS 45.0%
GAAGGTGAGGGAATTGGAAA+CGG + chr1.2:4631463-4631482 MS.gene044081:intron 45.0%
GACGTTGAAGAGGGAGATTA+GGG - chr1.2:4631079-4631098 None:intergenic 45.0%
GATGGACCAAAAAGACGATG+CGG + chr1.2:4631560-4631579 MS.gene044081:CDS 45.0%
GCTTCTTCTTGACGTTGAAG+AGG - chr1.2:4631089-4631108 None:intergenic 45.0%
GGAAAAAGGAACGGTGGAAA+TGG + chr1.2:4631523-4631542 MS.gene044081:CDS 45.0%
GGAAATGGAGGCTAGCATAA+TGG + chr1.2:4631538-4631557 MS.gene044081:CDS 45.0%
GGTTCATGGAGGTTGGAATT+TGG + chr1.2:4632919-4632938 MS.gene044081:CDS 45.0%
GTTTAAGGAAGACACACACG+TGG - chr1.2:4631727-4631746 None:intergenic 45.0%
TATGATAGCCTCACCTTCAC+CGG - chr1.2:4632706-4632725 None:intergenic 45.0%
TGACGTTGAAGAGGGAGATT+AGG - chr1.2:4631080-4631099 None:intergenic 45.0%
TGGTATGCTTAAGCTGGCAA+AGG + chr1.2:4631990-4632009 MS.gene044081:CDS 45.0%
! ATGGAGGCTAGCATAATGGA+TGG + chr1.2:4631542-4631561 MS.gene044081:CDS 45.0%
! TCGGCTGTGATGGAATACAA+AGG + chr1.2:4632750-4632769 MS.gene044081:CDS 45.0%
! TCTTGGTCTTCAAGAAGCAG+TGG + chr1.2:4632773-4632792 MS.gene044081:CDS 45.0%
!! TTCACTGGCTCGTTTAGTCA+TGG + chr1.2:4631619-4631638 MS.gene044081:CDS 45.0%
!!! CTTGTTTTTGTAGGGTGTGG+AGG + chr1.2:4631930-4631949 MS.gene044081:intron 45.0%
!! TTCAATTATTAATTAATTAT+TGG + chr1.2:4632322-4632341 MS.gene044081:intron 5.0%
AACATGTGTGTGTGGGTACG+AGG - chr1.2:4631247-4631266 None:intergenic 50.0%
ACTCACCTTTGATAGGAGCC+GGG + chr1.2:4632646-4632665 MS.gene044081:CDS 50.0%
GAAGACACACACGTGGGAAT+AGG - chr1.2:4631720-4631739 None:intergenic 50.0%
GAAGGTGAGGCTATCATACG+TGG + chr1.2:4632708-4632727 MS.gene044081:CDS 50.0%
GACTCACCTTTGATAGGAGC+CGG + chr1.2:4632645-4632664 MS.gene044081:CDS 50.0%
GGCTATCATACGTGGCACTT+TGG + chr1.2:4632716-4632735 MS.gene044081:CDS 50.0%
GTCTACGCACCTGAAACAGT+CGG + chr1.2:4632537-4632556 MS.gene044081:CDS 50.0%
GTTTAGGGGATGTGCTTCTG+AGG + chr1.2:4632893-4632912 MS.gene044081:CDS 50.0%
TAGTTGTGGACCGTGAAGGA+AGG + chr1.2:4632565-4632584 MS.gene044081:CDS 50.0%
TCAGGTGCGTAGACACTCAT+TGG - chr1.2:4632531-4632550 None:intergenic 50.0%
TCTACGCACCTGAAACAGTC+GGG + chr1.2:4632538-4632557 MS.gene044081:CDS 50.0%
TGCCCTGACGGAAAAAGGAA+CGG + chr1.2:4631514-4631533 MS.gene044081:CDS 50.0%
TTTGTAGGGTGTGGAGGTTC+TGG + chr1.2:4631936-4631955 MS.gene044081:intron 50.0%
! CACCGTTCCTTTTTCCGTCA+GGG - chr1.2:4631519-4631538 None:intergenic 50.0%
! TGCTTCTGAGGATGGGTTCA+TGG + chr1.2:4632905-4632924 MS.gene044081:CDS 50.0%
! TTCTGAGGATGGGTTCATGG+AGG + chr1.2:4632908-4632927 MS.gene044081:CDS 50.0%
AACAGTCGGGTGCGTAGTTG+TGG + chr1.2:4632551-4632570 MS.gene044081:CDS 55.0%
AAGGTTACACGCGTAGGTCC+CGG - chr1.2:4632667-4632686 None:intergenic 55.0%
ACCTACGCGTGTAACCTTGC+TGG + chr1.2:4632669-4632688 MS.gene044081:CDS 55.0%
ACTACGCACCCGACTGTTTC+AGG - chr1.2:4632549-4632568 None:intergenic 55.0%
CAGCAGCACACCTTCCTTCA+CGG - chr1.2:4632578-4632597 None:intergenic 55.0%
CCTGACGGAAAAAGGAACGG+TGG + chr1.2:4631517-4631536 MS.gene044081:CDS 55.0%
GGATCTGCCCTGACGGAAAA+AGG + chr1.2:4631509-4631528 MS.gene044081:CDS 55.0%
GGTTGGGCAATTGCTGTGCA+TGG + chr1.2:4631038-4631057 MS.gene044081:CDS 55.0%
TAGGTCCCGGCTCCTATCAA+AGG - chr1.2:4632654-4632673 None:intergenic 55.0%
TGCGTAGTTGTGGACCGTGA+AGG + chr1.2:4632561-4632580 MS.gene044081:CDS 55.0%
TGGGCAATTGCTGTGCATGG+TGG + chr1.2:4631041-4631060 MS.gene044081:CDS 55.0%
TGTGTGGGTACGAGGAAGTG+TGG - chr1.2:4631239-4631258 None:intergenic 55.0%
! ACGGCACCGCATCGTCTTTT+TGG - chr1.2:4631569-4631588 None:intergenic 55.0%
! AGGGGATGTGCTTCTGAGGA+TGG + chr1.2:4632897-4632916 MS.gene044081:CDS 55.0%
! ATTGCTGTGCATGGTGGTGC+TGG + chr1.2:4631047-4631066 MS.gene044081:CDS 55.0%
! CCACCGTTCCTTTTTCCGTC+AGG - chr1.2:4631520-4631539 None:intergenic 55.0%
! GAGGATGGGTTCATGGAGGT+TGG + chr1.2:4632912-4632931 MS.gene044081:CDS 55.0%
! GGGGATGTGCTTCTGAGGAT+GGG + chr1.2:4632898-4632917 MS.gene044081:CDS 55.0%
CATTAGCCCGCCTGTTGACG+TGG - chr1.2:4632601-4632620 None:intergenic 60.0%
CTTGCTGGCGTCTCATGCAC+CGG + chr1.2:4632684-4632703 MS.gene044081:CDS 60.0%
GCCAGCAAGGTTACACGCGT+AGG - chr1.2:4632673-4632692 None:intergenic 60.0%
! CACTTTGGCGCGAGAGGTGT+CGG + chr1.2:4632731-4632750 MS.gene044081:CDS 60.0%
! CTCATGCACCGGTGAAGGTG+AGG + chr1.2:4632695-4632714 MS.gene044081:CDS 60.0%
! TGTGCTGCTGCCACGTCAAC+AGG + chr1.2:4632588-4632607 MS.gene044081:CDS 60.0%
!! AGACGATGCGGTGCCGTTTC+AGG + chr1.2:4631572-4631591 MS.gene044081:CDS 60.0%
ACGTGGCACTTTGGCGCGAG+AGG + chr1.2:4632725-4632744 MS.gene044081:CDS 65.0%
CGGTGCATGAGACGCCAGCA+AGG - chr1.2:4632686-4632705 None:intergenic 65.0%
CTGCTGCCACGTCAACAGGC+GGG + chr1.2:4632592-4632611 MS.gene044081:CDS 65.0%
GCTGCTGCCACGTCAACAGG+CGG + chr1.2:4632591-4632610 MS.gene044081:CDS 65.0%
GGGTCGTGGATCTGCCCTGA+CGG + chr1.2:4631502-4631521 MS.gene044081:CDS 65.0%
! GGCGTCTCATGCACCGGTGA+AGG + chr1.2:4632690-4632709 MS.gene044081:CDS 65.0%
!! GCGAGAGGTGTCGGCTGTGA+TGG + chr1.2:4632740-4632759 MS.gene044081:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 4631032 4632947 4631032 ID=MS.gene044081
chr1.2 mRNA 4631032 4632947 4631032 ID=MS.gene044081.t1;Parent=MS.gene044081
chr1.2 exon 4631032 4631193 4631032 ID=MS.gene044081.t1.exon1;Parent=MS.gene044081.t1
chr1.2 CDS 4631032 4631193 4631032 ID=cds.MS.gene044081.t1;Parent=MS.gene044081.t1
chr1.2 exon 4631467 4631700 4631467 ID=MS.gene044081.t1.exon2;Parent=MS.gene044081.t1
chr1.2 CDS 4631467 4631700 4631467 ID=cds.MS.gene044081.t1;Parent=MS.gene044081.t1
chr1.2 exon 4631943 4632029 4631943 ID=MS.gene044081.t1.exon3;Parent=MS.gene044081.t1
chr1.2 CDS 4631943 4632029 4631943 ID=cds.MS.gene044081.t1;Parent=MS.gene044081.t1
chr1.2 exon 4632453 4632947 4632453 ID=MS.gene044081.t1.exon4;Parent=MS.gene044081.t1
chr1.2 CDS 4632453 4632947 4632453 ID=cds.MS.gene044081.t1;Parent=MS.gene044081.t1
Gene Sequence

>MS.gene044081

ATGGGTGGTTGGGCAATTGCTGTGCATGGTGGTGCTGGTGTTGACCCTAATCTCCCTCTTCAACGTCAAGAAGAAGCCAAACAACTTCTTACTCGTGTTCTCAATCTTGGCATCTCTGCTCTTCGTTCCAATCTTTCTGCCATCGATGTTGTTGAACTTGTGGTATGTGTTGTATATCTCACATCACATGTACCATAATATTGTCCACACTTCCTCGTACCCACACACACATGTTTCCATCTTGTCATGTCCCTTTTTTGTTCCTTTTCCACCGTTACCATTATTACTTCATGGCTACCATAACAAATAACCGTGAATCTAATACATGCATTGTCATTATGAACCAAACTACATGATTAACAAGAATATATTTTAACTATTCCAAGGAATTTGGAGGGGATTATTATTAATATTTATTTATTTTTGTTATTGAAGGTGAGGGAATTGGAAACGGATCCATTATTCAATTCGGGTCGTGGATCTGCCCTGACGGAAAAAGGAACGGTGGAAATGGAGGCTAGCATAATGGATGGACCAAAAAGACGATGCGGTGCCGTTTCAGGTTTAACCACAGTAAAAAATCCAATTTCACTGGCTCGTTTAGTCATGGATAAATCTCCTCATTCCTATCTTGCTTTTAACGGCGCTGAAGATTTTGCTAGACAACAGGTTCGTGTTCTCTTTACCTATTCCCACGTGTGTGTCTTCCTTAAACAACGTTAACCAAAAAAACCAAATCTCTGCAATTTTTTTTTTTGTTTGTTTTATCTTTATTTACAAAAAAATAGGAAAAGATATTTTATTTTTTGGGTTGGTGTTGGAACTTTGCTAAGGAGAATCTGTTGATTATTACGAATTATTAATTTATTATTAGTGCTAAATTACTTAAATTATTTTACTTGTTTTTGTAGGGTGTGGAGGTTCTGGACAATGAGTACTTTATCACCGCTGACAATGTTGGTATGCTTAAGCTGGCAAAGGAAGCAAATACAATCTTGGTACGTAATTATTATCAAATTCAACCATGGATCTTGAATTATTATTACATTGTACGGGTTAAAAGGGGTCCAGAGTGTGTGTTTTTCTGAAAAGGGTCTTTTCACATATTGAAATAAACGTGGGTAAAAATTATTTTCAGATTGGGATTCTTTTACAAAGTAAAGAAATATTGTATTCATGCATAGTTTGGACCAATTAGTACACTTTCTCTTTATTTAATATATATTTATATCTACCAAACTCTTTTGTTTATAAACAAATCTCAAACTACCAATGAAGACAAAGTGTTTGTTCAATTATTAATTAATTATTGGTAGCAATATGATTCTTTCATTCATTTTTTACGGTATCTTTCTTTTCATATTTAATTTACAATTTGAAACAACATAATTAATTAATTAATTATTGATTGTGTGCTGCAGTTTGATTATCGGATTCCATCTGGATACGAAACATGTGGTGCAGCAGTTGAAAGTCCAATGAAAATGAATGGACTTCCAATGAGTGTCTACGCACCTGAAACAGTCGGGTGCGTAGTTGTGGACCGTGAAGGAAGGTGTGCTGCTGCCACGTCAACAGGCGGGCTAATGAACAAAATGATTGGTCGGATTGGTGACTCACCTTTGATAGGAGCCGGGACCTACGCGTGTAACCTTGCTGGCGTCTCATGCACCGGTGAAGGTGAGGCTATCATACGTGGCACTTTGGCGCGAGAGGTGTCGGCTGTGATGGAATACAAAGGTCTTGGTCTTCAAGAAGCAGTGGATTTTGTCATTAAGAATCGTTTGGATGAAGGATATGCTGGACTCATTGCTGTTTCTAGTAAAGGAGAAGTTGCTTATGGATTTAACTGCAATGGCATGTTTAGGGGATGTGCTTCTGAGGATGGGTTCATGGAGGTTGGAATTTGGGAGTAG
Protein sequence

>MS.gene044081.t1

MGGWAIAVHGGAGVDPNLPLQRQEEAKQLLTRVLNLGISALRSNLSAIDVVELVVRELETDPLFNSGRGSALTEKGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFNGAEDFARQQGVEVLDNEYFITADNVGMLKLAKEANTILFDYRIPSGYETCGAAVESPMKMNGLPMSVYAPETVGCVVVDREGRCAAATSTGGLMNKMIGRIGDSPLIGAGTYACNLAGVSCTGEGEAIIRGTLAREVSAVMEYKGLGLQEAVDFVIKNRLDEGYAGLIAVSSKGEVAYGFNCNGMFRGCASEDGFMEVGIWE