Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048793.t1 | XP_024629048.1 | 96.3 | 272 | 10 | 0 | 1 | 272 | 1 | 272 | 7.30E-151 | 543.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048793.t1 | Q9FMS6 | 64.3 | 266 | 91 | 3 | 9 | 270 | 12 | 277 | 1.9e-91 | 337.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048793.t1 | I3SR57 | 96.3 | 272 | 10 | 0 | 1 | 272 | 1 | 272 | 5.3e-151 | 543.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene41100 | MS.gene048793 | PPI |
| MS.gene048793 | MS.gene007154 | PPI |
| MS.gene048793 | MS.gene025628 | PPI |
| MS.gene000609 | MS.gene048793 | PPI |
| MS.gene048793 | MS.gene065707 | PPI |
| MS.gene065708 | MS.gene048793 | PPI |
| MS.gene048793 | MS.gene21541 | PPI |
| MS.gene053225 | MS.gene048793 | PPI |
| MS.gene048793 | MS.gene41097 | PPI |
| MS.gene034815 | MS.gene048793 | PPI |
| MS.gene025629 | MS.gene048793 | PPI |
| MS.gene034250 | MS.gene048793 | PPI |
| MS.gene034816 | MS.gene048793 | PPI |
| MS.gene72977 | MS.gene048793 | PPI |
| MS.gene065709 | MS.gene048793 | PPI |
| MS.gene72978 | MS.gene048793 | PPI |
| MS.gene048793 | MS.gene41101 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048793.t1 | AT5G22250 | 64.286 | 266 | 91 | 3 | 9 | 270 | 12 | 277 | 3.02e-119 | 342 |
| MS.gene048793.t1 | AT3G44260 | 61.509 | 265 | 97 | 3 | 9 | 270 | 17 | 279 | 1.45e-116 | 336 |
| MS.gene048793.t1 | AT2G32070 | 50.746 | 268 | 127 | 5 | 4 | 270 | 5 | 268 | 2.96e-92 | 274 |
| MS.gene048793.t1 | AT5G10960 | 50.936 | 267 | 127 | 4 | 4 | 270 | 5 | 267 | 2.08e-91 | 272 |
| MS.gene048793.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 1.17e-88 | 265 |
| MS.gene048793.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 1.17e-88 | 265 |
| MS.gene048793.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 1.95e-88 | 265 |
| MS.gene048793.t1 | AT1G15920 | 47.407 | 270 | 134 | 5 | 5 | 269 | 10 | 276 | 6.17e-81 | 245 |
| MS.gene048793.t1 | AT1G15920 | 47.407 | 270 | 134 | 5 | 5 | 269 | 10 | 276 | 6.17e-81 | 245 |
| MS.gene048793.t1 | AT1G61470 | 37.037 | 243 | 130 | 7 | 13 | 249 | 4 | 229 | 1.21e-38 | 137 |
| MS.gene048793.t1 | AT1G06450 | 33.700 | 273 | 157 | 9 | 1 | 268 | 1 | 254 | 1.94e-38 | 138 |
| MS.gene048793.t1 | AT1G27820 | 35.510 | 245 | 143 | 5 | 7 | 248 | 2 | 234 | 5.49e-34 | 125 |
| MS.gene048793.t1 | AT3G44240 | 35.683 | 227 | 131 | 7 | 25 | 249 | 3 | 216 | 9.12e-33 | 120 |
| MS.gene048793.t1 | AT1G27890 | 35.565 | 239 | 139 | 5 | 13 | 248 | 3 | 229 | 1.24e-32 | 121 |
Find 63 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCACAGCGCTTATGATTT+TGG | 0.104590 | 4.3:+6141327 | MS.gene048793:CDS |
| AATGGCAACCTTCCTCATTT+TGG | 0.121265 | 4.3:+6141099 | MS.gene048793:CDS |
| ATCAAATTCAACCTCTAAAT+TGG | 0.207989 | 4.3:-6140896 | None:intergenic |
| AAACACGCTGGGGTACTATT+TGG | 0.216392 | 4.3:+6141624 | MS.gene048793:CDS |
| GCTGAATTGATGCTCACTTC+TGG | 0.258268 | 4.3:+6141270 | MS.gene048793:CDS |
| TATTCAACAAATCAGTTGTT+TGG | 0.275594 | 4.3:+6141298 | MS.gene048793:CDS |
| CAATCATCATCCGTCAAGTT+TGG | 0.286714 | 4.3:+6140869 | MS.gene048793:CDS |
| AACGGTTCGGCAGATTCCTT+CGG | 0.352418 | 4.3:-6141371 | None:intergenic |
| CTTAACGTGGGTCGGGTTGC+TGG | 0.360050 | 4.3:+6141510 | MS.gene048793:CDS |
| AGATGACGGCGGTCAACTTT+GGG | 0.368577 | 4.3:-6140991 | None:intergenic |
| ATTCAACAAATCAGTTGTTT+GGG | 0.389610 | 4.3:+6141299 | MS.gene048793:CDS |
| ACGGTTCGGCAGATTCCTTC+GGG | 0.395658 | 4.3:-6141370 | None:intergenic |
| GGACAAATAGCCGCTACATA+TGG | 0.399289 | 4.3:+6141121 | MS.gene048793:CDS |
| AATCATCATCCGTCAAGTTT+GGG | 0.414340 | 4.3:+6140870 | MS.gene048793:CDS |
| AAGATGACGGCGGTCAACTT+TGG | 0.418710 | 4.3:-6140992 | None:intergenic |
| GAATCTGCCGAACCGTTTAG+AGG | 0.420121 | 4.3:+6141377 | MS.gene048793:CDS |
| AGTTTGGGCATCCAATTTAG+AGG | 0.424325 | 4.3:+6140885 | MS.gene048793:CDS |
| TCCATGGATACTGAATTTCC+AGG | 0.437255 | 4.3:+6140955 | MS.gene048793:CDS |
| GGTTGCCATTACCATCAGAT+AGG | 0.447285 | 4.3:-6141086 | None:intergenic |
| CAAACTTGACGGATGATGAT+TGG | 0.450788 | 4.3:-6140868 | None:intergenic |
| TACTCATTTCTTGAATAATG+AGG | 0.477666 | 4.3:+6141596 | MS.gene048793:CDS |
| TGACCACTATCGTTACTTGA+AGG | 0.492905 | 4.3:+6141023 | MS.gene048793:CDS |
| TTCAAGCGCAATTTCTCTCA+TGG | 0.499679 | 4.3:+6141228 | MS.gene048793:CDS |
| CAATTGAGCCTTGGTTGTAA+AGG | 0.504025 | 4.3:-6141179 | None:intergenic |
| GACAAATAGCCGCTACATAT+GGG | 0.504514 | 4.3:+6141122 | MS.gene048793:CDS |
| TGCGCTTGAAGTCAATCCCT+TGG | 0.505546 | 4.3:-6141215 | None:intergenic |
| TCAATATCCTTTCATCTCCA+TGG | 0.506697 | 4.3:+6140939 | MS.gene048793:CDS |
| TACAACACTTAACGTGGGTC+GGG | 0.508105 | 4.3:+6141503 | MS.gene048793:CDS |
| AATTGGATGCCCAAACTTGA+CGG | 0.510297 | 4.3:-6140879 | None:intergenic |
| ACCTGGAAATTCAGTATCCA+TGG | 0.514112 | 4.3:-6140956 | None:intergenic |
| AGGTTCTGCAACGCTTTGTA+CGG | 0.515634 | 4.3:+6141462 | MS.gene048793:CDS |
| CAGATAGGGTGATACCAACT+TGG | 0.523809 | 4.3:-6141071 | None:intergenic |
| GATATTGATTGACAACTTGA+CGG | 0.523978 | 4.3:-6140924 | None:intergenic |
| GCATGCATTTAAGAAAATGA+TGG | 0.528476 | 4.3:+6141572 | MS.gene048793:CDS |
| CTACAACACTTAACGTGGGT+CGG | 0.533742 | 4.3:+6141502 | MS.gene048793:CDS |
| CGGCTATTTGTCCCAAAATG+AGG | 0.540450 | 4.3:-6141111 | None:intergenic |
| GCTGGAAAGTCTCATCAAGC+TGG | 0.542113 | 4.3:+6141528 | MS.gene048793:CDS |
| ATTGACATGCTCTGCCGCCA+AGG | 0.544877 | 4.3:+6141198 | MS.gene048793:CDS |
| AGAGCATGTCAATTGAGCCT+TGG | 0.548111 | 4.3:-6141188 | None:intergenic |
| ATCACCCTATCTGATGGTAA+TGG | 0.551182 | 4.3:+6141081 | MS.gene048793:CDS |
| TTGACATGCTCTGCCGCCAA+GGG | 0.557345 | 4.3:+6141199 | MS.gene048793:CDS |
| GCTTGAAGTCAATCCCTTGG+CGG | 0.562753 | 4.3:-6141212 | None:intergenic |
| GCTCTTAAACTCATCCAAGT+TGG | 0.568904 | 4.3:+6141057 | MS.gene048793:CDS |
| ATGATATGAAACACATGATG+AGG | 0.569362 | 4.3:+6141442 | MS.gene048793:CDS |
| TTGGGTGAATAAATTACACC+TGG | 0.570783 | 4.3:-6140973 | None:intergenic |
| TTCTGCAACGCTTTGTACGG+TGG | 0.584961 | 4.3:+6141465 | MS.gene048793:CDS |
| ATTCAAAATGAACCAGCTGA+AGG | 0.587598 | 4.3:+6140840 | None:intergenic |
| GGTCAGAGGGTTTAAGATGA+CGG | 0.592880 | 4.3:-6141005 | None:intergenic |
| AAGTAACGATAGTGGTCAGA+GGG | 0.593594 | 4.3:-6141018 | None:intergenic |
| TAGTGAAGATATTGACCCGA+AGG | 0.595020 | 4.3:+6141355 | MS.gene048793:CDS |
| GTTGGTATCACCCTATCTGA+TGG | 0.596348 | 4.3:+6141075 | MS.gene048793:CDS |
| TCAGTATCCATGGAGATGAA+AGG | 0.598752 | 4.3:-6140946 | None:intergenic |
| GTTGCCATTACCATCAGATA+GGG | 0.603208 | 4.3:-6141085 | None:intergenic |
| TGACCTTCAAGTAACGATAG+TGG | 0.622378 | 4.3:-6141026 | None:intergenic |
| TATTTGTCCCAAAATGAGGA+AGG | 0.623706 | 4.3:-6141107 | None:intergenic |
| AGTCGCTACAACACTTAACG+TGG | 0.625698 | 4.3:+6141497 | MS.gene048793:CDS |
| CAAGTAACGATAGTGGTCAG+AGG | 0.631701 | 4.3:-6141019 | None:intergenic |
| GAGCATGACCTTTACAACCA+AGG | 0.646186 | 4.3:+6141171 | MS.gene048793:CDS |
| GATCAGATAGTTTGTTGACG+TGG | 0.660381 | 4.3:+6141550 | MS.gene048793:CDS |
| AGTTGAACTCCCATATGTAG+CGG | 0.680885 | 4.3:-6141131 | None:intergenic |
| CATCAATTCAGCAAAACGTG+AGG | 0.687801 | 4.3:-6141259 | None:intergenic |
| GTCGCTACAACACTTAACGT+GGG | 0.701411 | 4.3:+6141498 | MS.gene048793:CDS |
| CAGAGGGTTTAAGATGACGG+CGG | 0.736962 | 4.3:-6141002 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTTAAAAAGTCCTCTAAA+CGG | - | chr4.3:6141392-6141411 | None:intergenic | 20.0% |
| ! | ATCAAATTCAACCTCTAAAT+TGG | - | chr4.3:6140899-6140918 | None:intergenic | 25.0% |
| ! | ATTCAACAAATCAGTTGTTT+GGG | + | chr4.3:6141299-6141318 | MS.gene048793:CDS | 25.0% |
| ! | TACTCATTTCTTGAATAATG+AGG | + | chr4.3:6141596-6141615 | MS.gene048793:CDS | 25.0% |
| ! | TATTCAACAAATCAGTTGTT+TGG | + | chr4.3:6141298-6141317 | MS.gene048793:CDS | 25.0% |
| ATGATATGAAACACATGATG+AGG | + | chr4.3:6141442-6141461 | MS.gene048793:CDS | 30.0% | |
| GCATGCATTTAAGAAAATGA+TGG | + | chr4.3:6141572-6141591 | MS.gene048793:CDS | 30.0% | |
| ! | GATATTGATTGACAACTTGA+CGG | - | chr4.3:6140927-6140946 | None:intergenic | 30.0% |
| AAAAAGTCCTCTAAACGGTT+CGG | - | chr4.3:6141387-6141406 | None:intergenic | 35.0% | |
| AATCATCATCCGTCAAGTTT+GGG | + | chr4.3:6140870-6140889 | MS.gene048793:CDS | 35.0% | |
| TATTTGTCCCAAAATGAGGA+AGG | - | chr4.3:6141110-6141129 | None:intergenic | 35.0% | |
| TCAATATCCTTTCATCTCCA+TGG | + | chr4.3:6140939-6140958 | MS.gene048793:CDS | 35.0% | |
| TTGGGTGAATAAATTACACC+TGG | - | chr4.3:6140976-6140995 | None:intergenic | 35.0% | |
| ! | TTCACAGCGCTTATGATTTT+GGG | + | chr4.3:6141328-6141347 | MS.gene048793:CDS | 35.0% |
| ! | TTTCACAGCGCTTATGATTT+TGG | + | chr4.3:6141327-6141346 | MS.gene048793:CDS | 35.0% |
| AATTGGATGCCCAAACTTGA+CGG | - | chr4.3:6140882-6140901 | None:intergenic | 40.0% | |
| ACCTGGAAATTCAGTATCCA+TGG | - | chr4.3:6140959-6140978 | None:intergenic | 40.0% | |
| AGTTGAACTCCCATATGTAG+CGG | - | chr4.3:6141134-6141153 | None:intergenic | 40.0% | |
| AGTTTGGGCATCCAATTTAG+AGG | + | chr4.3:6140885-6140904 | MS.gene048793:CDS | 40.0% | |
| ATCACCCTATCTGATGGTAA+TGG | + | chr4.3:6141081-6141100 | MS.gene048793:CDS | 40.0% | |
| CAATCATCATCCGTCAAGTT+TGG | + | chr4.3:6140869-6140888 | MS.gene048793:CDS | 40.0% | |
| CAATTGAGCCTTGGTTGTAA+AGG | - | chr4.3:6141182-6141201 | None:intergenic | 40.0% | |
| CATCAATTCAGCAAAACGTG+AGG | - | chr4.3:6141262-6141281 | None:intergenic | 40.0% | |
| GACAAATAGCCGCTACATAT+GGG | + | chr4.3:6141122-6141141 | MS.gene048793:CDS | 40.0% | |
| GCTCTTAAACTCATCCAAGT+TGG | + | chr4.3:6141057-6141076 | MS.gene048793:CDS | 40.0% | |
| GTTGCCATTACCATCAGATA+GGG | - | chr4.3:6141088-6141107 | None:intergenic | 40.0% | |
| TAGTGAAGATATTGACCCGA+AGG | + | chr4.3:6141355-6141374 | MS.gene048793:CDS | 40.0% | |
| TCAGTATCCATGGAGATGAA+AGG | - | chr4.3:6140949-6140968 | None:intergenic | 40.0% | |
| TCCATGGATACTGAATTTCC+AGG | + | chr4.3:6140955-6140974 | MS.gene048793:CDS | 40.0% | |
| TGACCACTATCGTTACTTGA+AGG | + | chr4.3:6141023-6141042 | MS.gene048793:CDS | 40.0% | |
| TGACCTTCAAGTAACGATAG+TGG | - | chr4.3:6141029-6141048 | None:intergenic | 40.0% | |
| TTCAAGCGCAATTTCTCTCA+TGG | + | chr4.3:6141228-6141247 | MS.gene048793:CDS | 40.0% | |
| ! | AAGTAACGATAGTGGTCAGA+GGG | - | chr4.3:6141021-6141040 | None:intergenic | 40.0% |
| ! | AATGGCAACCTTCCTCATTT+TGG | + | chr4.3:6141099-6141118 | MS.gene048793:CDS | 40.0% |
| ! | ATGGCAACCTTCCTCATTTT+GGG | + | chr4.3:6141100-6141119 | MS.gene048793:CDS | 40.0% |
| ! | CAAACTTGACGGATGATGAT+TGG | - | chr4.3:6140871-6140890 | None:intergenic | 40.0% |
| !! | GATCAGATAGTTTGTTGACG+TGG | + | chr4.3:6141550-6141569 | MS.gene048793:CDS | 40.0% |
| AATGAGGCTCAAAAACACGC+TGG | + | chr4.3:6141612-6141631 | MS.gene048793:CDS | 45.0% | |
| AGAGCATGTCAATTGAGCCT+TGG | - | chr4.3:6141191-6141210 | None:intergenic | 45.0% | |
| AGGTTCTGCAACGCTTTGTA+CGG | + | chr4.3:6141462-6141481 | MS.gene048793:CDS | 45.0% | |
| AGTCGCTACAACACTTAACG+TGG | + | chr4.3:6141497-6141516 | MS.gene048793:CDS | 45.0% | |
| ATGAGGCTCAAAAACACGCT+GGG | + | chr4.3:6141613-6141632 | MS.gene048793:CDS | 45.0% | |
| CAGATAGGGTGATACCAACT+TGG | - | chr4.3:6141074-6141093 | None:intergenic | 45.0% | |
| CGGCTATTTGTCCCAAAATG+AGG | - | chr4.3:6141114-6141133 | None:intergenic | 45.0% | |
| CTACAACACTTAACGTGGGT+CGG | + | chr4.3:6141502-6141521 | MS.gene048793:CDS | 45.0% | |
| GAGCATGACCTTTACAACCA+AGG | + | chr4.3:6141171-6141190 | MS.gene048793:CDS | 45.0% | |
| GCTGAATTGATGCTCACTTC+TGG | + | chr4.3:6141270-6141289 | MS.gene048793:CDS | 45.0% | |
| GGACAAATAGCCGCTACATA+TGG | + | chr4.3:6141121-6141140 | MS.gene048793:CDS | 45.0% | |
| GGTTGCCATTACCATCAGAT+AGG | - | chr4.3:6141089-6141108 | None:intergenic | 45.0% | |
| GTCGCTACAACACTTAACGT+GGG | + | chr4.3:6141498-6141517 | MS.gene048793:CDS | 45.0% | |
| GTTGGTATCACCCTATCTGA+TGG | + | chr4.3:6141075-6141094 | MS.gene048793:CDS | 45.0% | |
| TACAACACTTAACGTGGGTC+GGG | + | chr4.3:6141503-6141522 | MS.gene048793:CDS | 45.0% | |
| ! | AAACACGCTGGGGTACTATT+TGG | + | chr4.3:6141624-6141643 | MS.gene048793:CDS | 45.0% |
| ! | CAAGTAACGATAGTGGTCAG+AGG | - | chr4.3:6141022-6141041 | None:intergenic | 45.0% |
| ! | GGTCAGAGGGTTTAAGATGA+CGG | - | chr4.3:6141008-6141027 | None:intergenic | 45.0% |
| !!! | TGATTGGCTTTTCCTTCAGC+TGG | - | chr4.3:6140855-6140874 | None:intergenic | 45.0% |
| !!! | AATATTTTAACAATATTATT+TGG | + | chr4.3:6141408-6141427 | MS.gene048793:CDS | 5.0% |
| GAATCTGCCGAACCGTTTAG+AGG | + | chr4.3:6141377-6141396 | MS.gene048793:CDS | 50.0% | |
| GCTGGAAAGTCTCATCAAGC+TGG | + | chr4.3:6141528-6141547 | MS.gene048793:CDS | 50.0% | |
| GCTTGAAGTCAATCCCTTGG+CGG | - | chr4.3:6141215-6141234 | None:intergenic | 50.0% | |
| TGAGGCTCAAAAACACGCTG+GGG | + | chr4.3:6141614-6141633 | MS.gene048793:CDS | 50.0% | |
| TGCGCTTGAAGTCAATCCCT+TGG | - | chr4.3:6141218-6141237 | None:intergenic | 50.0% | |
| TTCTGCAACGCTTTGTACGG+TGG | + | chr4.3:6141465-6141484 | MS.gene048793:CDS | 50.0% | |
| ! | AACGGTTCGGCAGATTCCTT+CGG | - | chr4.3:6141374-6141393 | None:intergenic | 50.0% |
| ! | AAGATGACGGCGGTCAACTT+TGG | - | chr4.3:6140995-6141014 | None:intergenic | 50.0% |
| ! | AGATGACGGCGGTCAACTTT+GGG | - | chr4.3:6140994-6141013 | None:intergenic | 50.0% |
| ! | CAGAGGGTTTAAGATGACGG+CGG | - | chr4.3:6141005-6141024 | None:intergenic | 50.0% |
| ATTGACATGCTCTGCCGCCA+AGG | + | chr4.3:6141198-6141217 | MS.gene048793:CDS | 55.0% | |
| TTGACATGCTCTGCCGCCAA+GGG | + | chr4.3:6141199-6141218 | MS.gene048793:CDS | 55.0% | |
| ! | ACGGTTCGGCAGATTCCTTC+GGG | - | chr4.3:6141373-6141392 | None:intergenic | 55.0% |
| ! | CTTAACGTGGGTCGGGTTGC+TGG | + | chr4.3:6141510-6141529 | MS.gene048793:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 6140847 | 6141665 | 6140847 | ID=MS.gene048793 |
| chr4.3 | mRNA | 6140847 | 6141665 | 6140847 | ID=MS.gene048793.t1;Parent=MS.gene048793 |
| chr4.3 | exon | 6140847 | 6141665 | 6140847 | ID=MS.gene048793.t1.exon1;Parent=MS.gene048793.t1 |
| chr4.3 | CDS | 6140847 | 6141665 | 6140847 | ID=cds.MS.gene048793.t1;Parent=MS.gene048793.t1 |
| Gene Sequence |
| Protein sequence |
