Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048794.t1 | XP_024629047.1 | 97.4 | 272 | 7 | 0 | 1 | 272 | 1 | 272 | 3.50E-153 | 550.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048794.t1 | Q9LXM2 | 63.0 | 265 | 93 | 3 | 9 | 270 | 17 | 279 | 2.9e-92 | 339.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048794.t1 | I3S8F5 | 97.4 | 272 | 7 | 0 | 1 | 272 | 1 | 272 | 2.5e-153 | 550.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene025629 | MS.gene048794 | PPI |
| MS.gene21541 | MS.gene048794 | PPI |
| MS.gene034250 | MS.gene048794 | PPI |
| MS.gene41100 | MS.gene048794 | PPI |
| MS.gene72977 | MS.gene048794 | PPI |
| MS.gene025628 | MS.gene048794 | PPI |
| MS.gene065709 | MS.gene048794 | PPI |
| MS.gene007154 | MS.gene048794 | PPI |
| MS.gene000609 | MS.gene048794 | PPI |
| MS.gene065707 | MS.gene048794 | PPI |
| MS.gene41101 | MS.gene048794 | PPI |
| MS.gene41097 | MS.gene048794 | PPI |
| MS.gene065708 | MS.gene048794 | PPI |
| MS.gene053225 | MS.gene048794 | PPI |
| MS.gene034816 | MS.gene048794 | PPI |
| MS.gene72978 | MS.gene048794 | PPI |
| MS.gene034815 | MS.gene048794 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048794.t1 | AT3G44260 | 63.019 | 265 | 93 | 3 | 9 | 270 | 17 | 279 | 2.33e-119 | 343 |
| MS.gene048794.t1 | AT5G22250 | 64.286 | 266 | 91 | 3 | 9 | 270 | 12 | 277 | 2.77e-119 | 342 |
| MS.gene048794.t1 | AT2G32070 | 51.493 | 268 | 125 | 5 | 4 | 270 | 5 | 268 | 1.83e-93 | 277 |
| MS.gene048794.t1 | AT5G10960 | 51.311 | 267 | 126 | 4 | 4 | 270 | 5 | 267 | 1.68e-92 | 275 |
| MS.gene048794.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 1.61e-89 | 267 |
| MS.gene048794.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 1.61e-89 | 267 |
| MS.gene048794.t1 | AT1G80780 | 49.064 | 267 | 132 | 4 | 4 | 270 | 5 | 267 | 2.93e-89 | 266 |
| MS.gene048794.t1 | AT1G15920 | 48.519 | 270 | 131 | 5 | 5 | 269 | 10 | 276 | 3.45e-82 | 249 |
| MS.gene048794.t1 | AT1G15920 | 48.519 | 270 | 131 | 5 | 5 | 269 | 10 | 276 | 3.45e-82 | 249 |
| MS.gene048794.t1 | AT1G06450 | 34.432 | 273 | 155 | 9 | 1 | 268 | 1 | 254 | 4.69e-40 | 142 |
| MS.gene048794.t1 | AT1G61470 | 37.037 | 243 | 130 | 7 | 13 | 249 | 4 | 229 | 3.42e-38 | 135 |
| MS.gene048794.t1 | AT1G27820 | 35.102 | 245 | 144 | 5 | 7 | 248 | 2 | 234 | 1.09e-33 | 124 |
| MS.gene048794.t1 | AT3G44240 | 35.065 | 231 | 135 | 7 | 24 | 252 | 2 | 219 | 4.12e-33 | 121 |
| MS.gene048794.t1 | AT1G27890 | 34.728 | 239 | 141 | 5 | 13 | 248 | 3 | 229 | 7.68e-32 | 119 |
Find 63 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCACAGTGCTTATGATTT+TGG | 0.076417 | 4.3:+6138337 | MS.gene048794:CDS |
| AACGGTAACCTTCCTCATTT+TGG | 0.104000 | 4.3:+6138109 | MS.gene048794:CDS |
| AAACACGCCGGGGTCTTATT+TGG | 0.170808 | 4.3:+6138634 | MS.gene048794:CDS |
| GCTGAATTGATGCTCACTTC+TGG | 0.174511 | 4.3:+6138280 | MS.gene048794:CDS |
| CAATCATCATCCGTCAAGTT+TGG | 0.286714 | 4.3:+6137879 | MS.gene048794:CDS |
| TCTTCAACAAATCAGTTGTT+TGG | 0.295934 | 4.3:+6138308 | MS.gene048794:CDS |
| CTGAATTGATGCTCACTTCT+GGG | 0.307956 | 4.3:+6138281 | MS.gene048794:CDS |
| GGACAAATAACTGCTACATA+TGG | 0.328351 | 4.3:+6138131 | MS.gene048794:CDS |
| CTTAACGTGGGTCGGGTTGC+TGG | 0.360050 | 4.3:+6138520 | MS.gene048794:CDS |
| GAATTTGCCTAACCGCTTAG+AGG | 0.364570 | 4.3:+6138387 | MS.gene048794:CDS |
| AGATGACGGCGGTCAACTTT+GGG | 0.368577 | 4.3:-6138001 | None:intergenic |
| CTTCAACAAATCAGTTGTTT+GGG | 0.376564 | 4.3:+6138309 | MS.gene048794:CDS |
| AGCGGTTAGGCAAATTCCTT+CGG | 0.390068 | 4.3:-6138381 | None:intergenic |
| AATCATCATCCGTCAAGTTT+GGG | 0.414340 | 4.3:+6137880 | MS.gene048794:CDS |
| AAGATGACGGCGGTCAACTT+TGG | 0.418710 | 4.3:-6138002 | None:intergenic |
| GCGGTTAGGCAAATTCCTTC+GGG | 0.436779 | 4.3:-6138380 | None:intergenic |
| CAAACTTGACGGATGATGAT+TGG | 0.450788 | 4.3:-6137878 | None:intergenic |
| AGAGCATGTCAATTGAATCT+TGG | 0.452953 | 4.3:-6138198 | None:intergenic |
| AATTAGATGCCCAAACTTGA+CGG | 0.464834 | 4.3:-6137889 | None:intergenic |
| TTCGCTTGAAGTCAATCCCT+TGG | 0.469385 | 4.3:-6138225 | None:intergenic |
| GACAAATAACTGCTACATAT+GGG | 0.475547 | 4.3:+6138132 | MS.gene048794:CDS |
| CAATTGAATCTTGGTTGTAA+AGG | 0.483444 | 4.3:-6138189 | None:intergenic |
| TGACCACTATCGTTACTTGA+AGG | 0.492206 | 4.3:+6138033 | MS.gene048794:CDS |
| TACAACACTTAACGTGGGTC+GGG | 0.508105 | 4.3:+6138513 | MS.gene048794:CDS |
| GCTCTTAAGCTTATCCAAGT+TGG | 0.510756 | 4.3:+6138067 | MS.gene048794:CDS |
| GATATTGGTTGATAACTTGA+CGG | 0.511970 | 4.3:-6137934 | None:intergenic |
| CCATGGAGATGAAAGGATAT+TGG | 0.513144 | 4.3:-6137949 | None:intergenic |
| CAGTTATTTGTCCCAAAATG+AGG | 0.514189 | 4.3:-6138121 | None:intergenic |
| CCAATATCCTTTCATCTCCA+TGG | 0.518224 | 4.3:+6137949 | MS.gene048794:CDS |
| TTCAAGCGAAATTTATGTCA+TGG | 0.518374 | 4.3:+6138238 | MS.gene048794:CDS |
| ACCAGGGAATTCAGTGTCCA+TGG | 0.528331 | 4.3:-6137966 | None:intergenic |
| GCATGCATTTAAGAAAATGA+TGG | 0.528476 | 4.3:+6138582 | MS.gene048794:CDS |
| CTACAACACTTAACGTGGGT+CGG | 0.533742 | 4.3:+6138512 | MS.gene048794:CDS |
| CAGAGAGGGTGATACCAACT+TGG | 0.540291 | 4.3:-6138081 | None:intergenic |
| ATGATATGAAACACATGATG+AGG | 0.540345 | 4.3:+6138452 | MS.gene048794:CDS |
| GCTGGAAAGTCTCATCAAGC+TGG | 0.542113 | 4.3:+6138538 | MS.gene048794:CDS |
| AAGAAGTCCTCTAAGCGGTT+AGG | 0.544312 | 4.3:-6138394 | None:intergenic |
| GCTTGAAGTCAATCCCTTGG+CGG | 0.551836 | 4.3:-6138222 | None:intergenic |
| GCGAAATTTATGTCATGGTG+TGG | 0.555209 | 4.3:+6138243 | MS.gene048794:CDS |
| TTGACATGCTCTGCCGCCAA+GGG | 0.557345 | 4.3:+6138209 | MS.gene048794:CDS |
| ATTGACATGCTCTGCCGCCA+AGG | 0.558688 | 4.3:+6138208 | MS.gene048794:CDS |
| TACTTACTTCATGAATAATG+AGG | 0.569380 | 4.3:+6138606 | MS.gene048794:CDS |
| GTTGGTATCACCCTCTCTGA+TGG | 0.573766 | 4.3:+6138085 | MS.gene048794:CDS |
| TTACCTTCAAGTAACGATAG+TGG | 0.575347 | 4.3:-6138036 | None:intergenic |
| TCCATGGACACTGAATTCCC+TGG | 0.586485 | 4.3:+6137965 | MS.gene048794:CDS |
| ATTCAAAATGAACCAGCTGA+AGG | 0.592335 | 4.3:+6137850 | None:intergenic |
| GGTCAGAGGGTTTAAGATGA+CGG | 0.592880 | 4.3:-6138015 | None:intergenic |
| AAGTAACGATAGTGGTCAGA+GGG | 0.593594 | 4.3:-6138028 | None:intergenic |
| TATTTGTCCCAAAATGAGGA+AGG | 0.593755 | 4.3:-6138117 | None:intergenic |
| TGGTGAAGATCTTGACCCGA+AGG | 0.604460 | 4.3:+6138365 | MS.gene048794:CDS |
| TTCTAATCCAAATAAGACCC+CGG | 0.610017 | 4.3:-6138641 | None:intergenic |
| AGTCGCTACAACACTTAACG+TGG | 0.625698 | 4.3:+6138507 | MS.gene048794:CDS |
| CAAGTAACGATAGTGGTCAG+AGG | 0.631701 | 4.3:-6138029 | None:intergenic |
| ATCACCCTCTCTGATGGTAA+CGG | 0.635418 | 4.3:+6138091 | MS.gene048794:CDS |
| TTGGGTGAATAAATCACACC+AGG | 0.640282 | 4.3:-6137983 | None:intergenic |
| TCAGTGTCCATGGAGATGAA+AGG | 0.659721 | 4.3:-6137956 | None:intergenic |
| GATCAGATAGTTTGTTGACG+TGG | 0.660381 | 4.3:+6138560 | MS.gene048794:CDS |
| TATTTAAGAAGTCCTCTAAG+CGG | 0.683422 | 4.3:-6138399 | None:intergenic |
| GTTACCGTTACCATCAGAGA+GGG | 0.698499 | 4.3:-6138095 | None:intergenic |
| GTCGCTACAACACTTAACGT+GGG | 0.701411 | 4.3:+6138508 | MS.gene048794:CDS |
| GGTTACCGTTACCATCAGAG+AGG | 0.730463 | 4.3:-6138096 | None:intergenic |
| CAGAGGGTTTAAGATGACGG+CGG | 0.736962 | 4.3:-6138012 | None:intergenic |
| TGGGTGAATAAATCACACCA+GGG | 0.753086 | 4.3:-6137982 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATTTTAACAATATTGTT+TGG | + | chr4.3:6138418-6138437 | MS.gene048794:CDS | 10.0% |
| ! | TACTTACTTCATGAATAATG+AGG | + | chr4.3:6138606-6138625 | MS.gene048794:CDS | 25.0% |
| ATGATATGAAACACATGATG+AGG | + | chr4.3:6138452-6138471 | MS.gene048794:CDS | 30.0% | |
| CAATTGAATCTTGGTTGTAA+AGG | - | chr4.3:6138192-6138211 | None:intergenic | 30.0% | |
| CTTCAACAAATCAGTTGTTT+GGG | + | chr4.3:6138309-6138328 | MS.gene048794:CDS | 30.0% | |
| GACAAATAACTGCTACATAT+GGG | + | chr4.3:6138132-6138151 | MS.gene048794:CDS | 30.0% | |
| GCATGCATTTAAGAAAATGA+TGG | + | chr4.3:6138582-6138601 | MS.gene048794:CDS | 30.0% | |
| TATTTAAGAAGTCCTCTAAG+CGG | - | chr4.3:6138402-6138421 | None:intergenic | 30.0% | |
| TCTTCAACAAATCAGTTGTT+TGG | + | chr4.3:6138308-6138327 | MS.gene048794:CDS | 30.0% | |
| TTCAAGCGAAATTTATGTCA+TGG | + | chr4.3:6138238-6138257 | MS.gene048794:CDS | 30.0% | |
| ! | TTCACAGTGCTTATGATTTT+GGG | + | chr4.3:6138338-6138357 | MS.gene048794:CDS | 30.0% |
| ! | TTTCACAGTGCTTATGATTT+TGG | + | chr4.3:6138337-6138356 | MS.gene048794:CDS | 30.0% |
| !! | GATATTGGTTGATAACTTGA+CGG | - | chr4.3:6137937-6137956 | None:intergenic | 30.0% |
| !! | TGCTTATGATTTTGGGTATT+TGG | + | chr4.3:6138345-6138364 | MS.gene048794:CDS | 30.0% |
| AATCATCATCCGTCAAGTTT+GGG | + | chr4.3:6137880-6137899 | MS.gene048794:CDS | 35.0% | |
| AATTAGATGCCCAAACTTGA+CGG | - | chr4.3:6137892-6137911 | None:intergenic | 35.0% | |
| AGAGCATGTCAATTGAATCT+TGG | - | chr4.3:6138201-6138220 | None:intergenic | 35.0% | |
| CAGTTATTTGTCCCAAAATG+AGG | - | chr4.3:6138124-6138143 | None:intergenic | 35.0% | |
| GGACAAATAACTGCTACATA+TGG | + | chr4.3:6138131-6138150 | MS.gene048794:CDS | 35.0% | |
| TATTTGTCCCAAAATGAGGA+AGG | - | chr4.3:6138120-6138139 | None:intergenic | 35.0% | |
| TTACCTTCAAGTAACGATAG+TGG | - | chr4.3:6138039-6138058 | None:intergenic | 35.0% | |
| TTCTAATCCAAATAAGACCC+CGG | - | chr4.3:6138644-6138663 | None:intergenic | 35.0% | |
| CAATCATCATCCGTCAAGTT+TGG | + | chr4.3:6137879-6137898 | MS.gene048794:CDS | 40.0% | |
| CCAATATCCTTTCATCTCCA+TGG | + | chr4.3:6137949-6137968 | MS.gene048794:CDS | 40.0% | |
| CCATGGAGATGAAAGGATAT+TGG | - | chr4.3:6137952-6137971 | None:intergenic | 40.0% | |
| CTGAATTGATGCTCACTTCT+GGG | + | chr4.3:6138281-6138300 | MS.gene048794:CDS | 40.0% | |
| GCGAAATTTATGTCATGGTG+TGG | + | chr4.3:6138243-6138262 | MS.gene048794:CDS | 40.0% | |
| GCTCTTAAGCTTATCCAAGT+TGG | + | chr4.3:6138067-6138086 | MS.gene048794:CDS | 40.0% | |
| TGACCACTATCGTTACTTGA+AGG | + | chr4.3:6138033-6138052 | MS.gene048794:CDS | 40.0% | |
| TGGGTGAATAAATCACACCA+GGG | - | chr4.3:6137985-6138004 | None:intergenic | 40.0% | |
| TTGGGTGAATAAATCACACC+AGG | - | chr4.3:6137986-6138005 | None:intergenic | 40.0% | |
| ! | AACGGTAACCTTCCTCATTT+TGG | + | chr4.3:6138109-6138128 | MS.gene048794:CDS | 40.0% |
| ! | AAGTAACGATAGTGGTCAGA+GGG | - | chr4.3:6138031-6138050 | None:intergenic | 40.0% |
| ! | ACGGTAACCTTCCTCATTTT+GGG | + | chr4.3:6138110-6138129 | MS.gene048794:CDS | 40.0% |
| ! | AGGTTTTGCAACGCTTTGTA+CGG | + | chr4.3:6138472-6138491 | MS.gene048794:CDS | 40.0% |
| ! | CAAACTTGACGGATGATGAT+TGG | - | chr4.3:6137881-6137900 | None:intergenic | 40.0% |
| !! | GATCAGATAGTTTGTTGACG+TGG | + | chr4.3:6138560-6138579 | MS.gene048794:CDS | 40.0% |
| AAGAAGTCCTCTAAGCGGTT+AGG | - | chr4.3:6138397-6138416 | None:intergenic | 45.0% | |
| AATGAGGCTCAAAAACACGC+CGG | + | chr4.3:6138622-6138641 | MS.gene048794:CDS | 45.0% | |
| AGCGGTTAGGCAAATTCCTT+CGG | - | chr4.3:6138384-6138403 | None:intergenic | 45.0% | |
| AGTCGCTACAACACTTAACG+TGG | + | chr4.3:6138507-6138526 | MS.gene048794:CDS | 45.0% | |
| ATCACCCTCTCTGATGGTAA+CGG | + | chr4.3:6138091-6138110 | MS.gene048794:CDS | 45.0% | |
| CTACAACACTTAACGTGGGT+CGG | + | chr4.3:6138512-6138531 | MS.gene048794:CDS | 45.0% | |
| GAATTTGCCTAACCGCTTAG+AGG | + | chr4.3:6138387-6138406 | MS.gene048794:CDS | 45.0% | |
| GCTGAATTGATGCTCACTTC+TGG | + | chr4.3:6138280-6138299 | MS.gene048794:CDS | 45.0% | |
| GTCGCTACAACACTTAACGT+GGG | + | chr4.3:6138508-6138527 | MS.gene048794:CDS | 45.0% | |
| GTTACCGTTACCATCAGAGA+GGG | - | chr4.3:6138098-6138117 | None:intergenic | 45.0% | |
| TACAACACTTAACGTGGGTC+GGG | + | chr4.3:6138513-6138532 | MS.gene048794:CDS | 45.0% | |
| TCAGTGTCCATGGAGATGAA+AGG | - | chr4.3:6137959-6137978 | None:intergenic | 45.0% | |
| TTCGCTTGAAGTCAATCCCT+TGG | - | chr4.3:6138228-6138247 | None:intergenic | 45.0% | |
| ! | CAAGTAACGATAGTGGTCAG+AGG | - | chr4.3:6138032-6138051 | None:intergenic | 45.0% |
| ! | GGTCAGAGGGTTTAAGATGA+CGG | - | chr4.3:6138018-6138037 | None:intergenic | 45.0% |
| ! | TTTTGCAACGCTTTGTACGG+TGG | + | chr4.3:6138475-6138494 | MS.gene048794:CDS | 45.0% |
| !!! | TGATTGGCTTTTCCTTCAGC+TGG | - | chr4.3:6137865-6137884 | None:intergenic | 45.0% |
| ACCAGGGAATTCAGTGTCCA+TGG | - | chr4.3:6137969-6137988 | None:intergenic | 50.0% | |
| ATGAGGCTCAAAAACACGCC+GGG | + | chr4.3:6138623-6138642 | MS.gene048794:CDS | 50.0% | |
| CAGAGAGGGTGATACCAACT+TGG | - | chr4.3:6138084-6138103 | None:intergenic | 50.0% | |
| GCGGTTAGGCAAATTCCTTC+GGG | - | chr4.3:6138383-6138402 | None:intergenic | 50.0% | |
| GCTGGAAAGTCTCATCAAGC+TGG | + | chr4.3:6138538-6138557 | MS.gene048794:CDS | 50.0% | |
| GCTTGAAGTCAATCCCTTGG+CGG | - | chr4.3:6138225-6138244 | None:intergenic | 50.0% | |
| GGTTACCGTTACCATCAGAG+AGG | - | chr4.3:6138099-6138118 | None:intergenic | 50.0% | |
| GTTGGTATCACCCTCTCTGA+TGG | + | chr4.3:6138085-6138104 | MS.gene048794:CDS | 50.0% | |
| TCCATGGACACTGAATTCCC+TGG | + | chr4.3:6137965-6137984 | MS.gene048794:CDS | 50.0% | |
| TGGTGAAGATCTTGACCCGA+AGG | + | chr4.3:6138365-6138384 | MS.gene048794:CDS | 50.0% | |
| ! | AAGATGACGGCGGTCAACTT+TGG | - | chr4.3:6138005-6138024 | None:intergenic | 50.0% |
| ! | AGATGACGGCGGTCAACTTT+GGG | - | chr4.3:6138004-6138023 | None:intergenic | 50.0% |
| ! | CAGAGGGTTTAAGATGACGG+CGG | - | chr4.3:6138015-6138034 | None:intergenic | 50.0% |
| !! | AAACACGCCGGGGTCTTATT+TGG | + | chr4.3:6138634-6138653 | MS.gene048794:CDS | 50.0% |
| ATTGACATGCTCTGCCGCCA+AGG | + | chr4.3:6138208-6138227 | MS.gene048794:CDS | 55.0% | |
| TGAGGCTCAAAAACACGCCG+GGG | + | chr4.3:6138624-6138643 | MS.gene048794:CDS | 55.0% | |
| TTGACATGCTCTGCCGCCAA+GGG | + | chr4.3:6138209-6138228 | MS.gene048794:CDS | 55.0% | |
| ! | CTTAACGTGGGTCGGGTTGC+TGG | + | chr4.3:6138520-6138539 | MS.gene048794:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 6137857 | 6138675 | 6137857 | ID=MS.gene048794 |
| chr4.3 | mRNA | 6137857 | 6138675 | 6137857 | ID=MS.gene048794.t1;Parent=MS.gene048794 |
| chr4.3 | exon | 6137857 | 6138675 | 6137857 | ID=MS.gene048794.t1.exon1;Parent=MS.gene048794.t1 |
| chr4.3 | CDS | 6137857 | 6138675 | 6137857 | ID=cds.MS.gene048794.t1;Parent=MS.gene048794.t1 |
| Gene Sequence |
| Protein sequence |
