AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05035

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05035.t1 MTR_8g095240 88.028 426 46 3 1 422 1 425 0.0 708
MS.gene05035.t1 MTR_8g092410 51.220 369 114 9 101 422 32 381 5.87e-94 288
MS.gene05035.t1 MTR_8g092410 48.322 149 64 5 80 224 137 276 1.19e-29 119
MS.gene05035.t1 MTR_8g092410 37.821 156 60 3 26 153 129 275 1.16e-15 78.6
MS.gene05035.t1 MTR_8g092090 60.082 243 88 5 97 335 9 246 6.00e-82 252
MS.gene05035.t1 MTR_8g092090 49.286 280 87 6 1 265 6 245 2.40e-71 225
MS.gene05035.t1 MTR_4g107460 46.497 314 127 5 53 364 11 285 2.11e-74 236
MS.gene05035.t1 MTR_4g107460 47.887 284 109 6 8 276 13 272 1.04e-68 221
MS.gene05035.t1 MTR_4g107460 38.889 180 76 3 5 183 126 272 4.83e-22 96.7
MS.gene05035.t1 MTR_8g080860 50.000 266 121 3 71 335 17 271 8.75e-68 217
MS.gene05035.t1 MTR_8g080860 53.556 239 95 4 3 227 19 255 2.04e-64 208
MS.gene05035.t1 MTR_8g080860 42.308 208 105 3 1 194 63 269 1.63e-39 143
MS.gene05035.t1 MTR_8g080860 38.911 257 99 4 119 352 19 240 5.40e-38 139
MS.gene05035.t1 MTR_8g080860 50.000 266 121 3 71 335 13 267 1.23e-67 216
MS.gene05035.t1 MTR_8g080860 53.556 239 95 4 3 227 15 251 3.02e-64 208
MS.gene05035.t1 MTR_8g080860 42.308 208 105 3 1 194 59 265 2.08e-39 143
MS.gene05035.t1 MTR_8g080860 38.911 257 99 4 119 352 15 236 6.35e-38 139
MS.gene05035.t1 MTR_6g043150 37.386 329 195 3 3 331 9 326 2.80e-53 181
MS.gene05035.t1 MTR_6g043150 31.119 286 164 2 52 337 11 263 8.73e-30 118
MS.gene05035.t1 MTR_6g043150 32.367 207 138 2 146 351 4 209 5.24e-23 99.4
MS.gene05035.t1 MTR_6g043150 31.925 213 114 3 1 191 122 325 2.39e-18 85.9
MS.gene05035.t1 MTR_2g078470 35.475 358 174 6 3 337 9 332 3.31e-47 166
MS.gene05035.t1 MTR_2g078470 35.505 307 187 4 4 310 79 374 2.61e-45 161
MS.gene05035.t1 MTR_8g080310 58.824 153 61 2 126 277 6 157 1.38e-44 152
MS.gene05035.t1 MTR_8g080310 53.548 155 57 3 90 230 3 156 5.39e-37 132
MS.gene05035.t1 MTR_8g080310 47.436 156 68 2 43 184 2 157 1.27e-32 121
MS.gene05035.t1 MTR_8g080310 42.857 147 61 1 214 337 2 148 1.46e-25 102
MS.gene05035.t1 MTR_8g088930 35.191 341 161 9 119 414 12 337 3.59e-44 163
MS.gene05035.t1 MTR_8g088930 43.128 211 87 4 3 213 12 189 3.41e-39 149
MS.gene05035.t1 MTR_8g088930 35.191 341 161 9 119 414 12 337 3.66e-44 163
MS.gene05035.t1 MTR_8g088930 43.128 211 87 4 3 213 12 189 3.32e-39 149
MS.gene05035.t1 MTR_8g088930 34.897 341 162 9 119 414 12 337 1.38e-43 162
MS.gene05035.t1 MTR_8g088930 43.128 211 87 4 3 213 12 189 3.08e-39 150
MS.gene05035.t1 MTR_8g088930 34.897 341 162 9 119 414 12 337 1.44e-43 162
MS.gene05035.t1 MTR_8g088930 43.128 211 87 4 3 213 12 189 3.15e-39 150
MS.gene05035.t1 MTR_5g010840 37.456 283 149 8 2 279 109 368 1.98e-39 145
MS.gene05035.t1 MTR_5g010840 27.273 242 135 2 96 337 111 311 8.86e-16 79.0
MS.gene05035.t1 MTR_8g080350 36.889 225 119 4 3 213 125 340 1.63e-32 127
MS.gene05035.t1 MTR_8g080350 29.210 291 142 7 32 300 86 334 2.40e-21 95.5
MS.gene05035.t1 MTR_8g080350 40.845 142 61 2 218 336 99 240 2.51e-19 89.7
MS.gene05035.t1 MTR_8g080350 57.692 78 33 0 260 337 95 172 3.15e-19 89.4
MS.gene05035.t1 MTR_8g080340 43.976 166 60 3 7 172 22 154 6.43e-32 119
MS.gene05035.t1 MTR_8g080340 43.275 171 73 1 165 335 9 155 1.67e-29 113
MS.gene05035.t1 MTR_8g080340 47.015 134 60 3 94 227 17 139 2.78e-26 104
MS.gene05035.t1 MTR_5g058920 35.922 206 121 3 113 317 190 385 2.08e-25 107
MS.gene05035.t1 MTR_5g058920 34.956 226 107 4 35 253 174 366 1.02e-24 105
MS.gene05035.t1 MTR_5g058920 40.476 126 74 1 9 134 248 372 6.20e-17 82.8
MS.gene05035.t1 MTR_5g058920 32.240 183 91 3 2 161 195 367 5.59e-14 73.9
MS.gene05035.t1 MTR_5g058920 31.667 180 114 4 166 342 188 361 1.36e-12 69.3
MS.gene05035.t1 MTR_3g023310 48.120 133 55 2 141 273 8 126 4.67e-25 101
MS.gene05035.t1 MTR_3g023310 45.802 131 50 3 61 180 6 126 2.62e-23 96.7
MS.gene05035.t1 MTR_3g023310 46.667 120 60 3 110 227 9 126 1.81e-20 88.6
MS.gene05035.t1 MTR_3g023310 44.643 112 48 2 189 300 10 107 5.32e-16 75.9
MS.gene05035.t1 MTR_8g039100 35.938 192 113 3 9 200 58 239 1.53e-24 106
MS.gene05035.t1 MTR_8g039100 30.256 195 121 3 94 274 50 243 3.44e-17 84.3
MS.gene05035.t1 MTR_3g097260 36.000 200 95 3 8 207 59 225 3.17e-24 105
MS.gene05035.t1 MTR_7g451440 33.990 203 115 4 103 305 184 367 1.03e-23 103
MS.gene05035.t1 MTR_7g451440 35.028 177 99 4 53 214 194 369 4.75e-22 98.2
MS.gene05035.t1 MTR_7g451440 34.807 181 108 2 8 188 196 366 7.81e-22 97.4
MS.gene05035.t1 MTR_7g451440 31.492 181 119 3 162 340 177 354 8.37e-13 70.1
MS.gene05035.t1 MTR_3g072650 35.789 190 111 4 2 190 183 362 3.46e-23 100
MS.gene05035.t1 MTR_3g072650 35.135 185 110 3 115 299 180 354 7.69e-21 94.0
MS.gene05035.t1 MTR_3g072650 31.280 211 112 2 38 248 172 349 1.26e-20 93.6
MS.gene05035.t1 MTR_3g072650 30.928 194 124 3 87 280 175 358 6.63e-13 70.1
MS.gene05035.t1 MTR_3g072650 35.789 190 111 4 2 190 183 362 5.06e-23 100
MS.gene05035.t1 MTR_3g072650 35.135 185 110 3 115 299 180 354 7.27e-21 94.4
MS.gene05035.t1 MTR_3g072650 31.280 211 112 2 38 248 172 349 1.74e-20 93.6
MS.gene05035.t1 MTR_3g072650 30.412 194 125 2 87 280 175 358 5.22e-13 70.9
MS.gene05035.t1 MTR_8g007500 34.021 194 110 3 116 305 193 372 2.99e-22 99.0
MS.gene05035.t1 MTR_8g007500 32.955 176 104 2 53 214 199 374 3.60e-20 92.8
MS.gene05035.t1 MTR_8g007500 31.455 213 113 3 70 282 193 372 8.44e-18 85.5
MS.gene05035.t1 MTR_8g007500 36.290 124 78 1 9 132 248 370 3.94e-15 77.4
MS.gene05035.t1 MTR_8g007500 30.435 184 95 3 2 162 195 368 5.67e-13 70.9
MS.gene05035.t1 MTR_8g007500 31.053 190 105 4 151 340 196 359 1.39e-11 66.6
MS.gene05035.t1 MTR_1g062740 34.659 176 100 3 9 170 56 230 1.16e-21 98.2
MS.gene05035.t1 MTR_1g062740 34.503 171 100 4 119 288 50 209 1.70e-19 91.7
MS.gene05035.t1 MTR_1g062740 27.424 361 213 9 72 407 49 385 5.02e-19 90.1
MS.gene05035.t1 MTR_1g062740 36.000 125 79 1 8 132 101 224 1.05e-13 73.6
MS.gene05035.t1 MTR_1g050740 33.659 205 103 3 9 213 66 237 1.66e-21 97.8
MS.gene05035.t1 MTR_1g050740 29.032 217 131 4 76 270 32 247 7.36e-18 86.7
MS.gene05035.t1 MTR_1g050740 31.953 169 105 2 2 170 82 240 8.24e-17 83.2
MS.gene05035.t1 MTR_4g099180 31.683 202 128 3 116 317 168 359 3.13e-21 95.5
MS.gene05035.t1 MTR_4g099180 30.769 247 122 6 31 271 161 364 8.70e-21 94.4
MS.gene05035.t1 MTR_4g099180 30.270 185 96 4 1 162 169 343 1.81e-13 72.0
MS.gene05035.t1 MTR_4g099180 35.484 124 79 1 9 132 223 345 4.52e-13 70.9
MS.gene05035.t1 MTR_4g099180 30.337 178 121 2 163 340 160 334 3.97e-12 68.2
MS.gene05035.t1 MTR_3g073100 35.678 199 94 4 9 206 62 227 1.21e-20 95.1
MS.gene05035.t1 MTR_3g073100 29.778 225 123 4 51 273 57 248 2.91e-16 81.6
MS.gene05035.t1 MTR_3g073100 33.121 157 95 2 3 159 80 226 5.07e-13 71.2
MS.gene05035.t1 MTR_5g459450 34.197 193 94 5 9 201 74 233 3.43e-20 93.6
MS.gene05035.t1 MTR_5g459450 35.185 162 96 1 49 210 67 219 1.20e-18 89.0
MS.gene05035.t1 MTR_5g459450 36.735 147 90 3 2 147 90 234 1.41e-13 73.2
MS.gene05035.t1 MTR_5g459450 28.079 203 111 3 89 289 59 228 1.93e-12 69.7
MS.gene05035.t1 MTR_3g014650 33.333 171 90 3 34 180 106 276 7.33e-20 92.4
MS.gene05035.t1 MTR_3g014650 30.570 193 119 5 94 273 86 276 2.92e-17 84.7
MS.gene05035.t1 MTR_3g014650 39.516 124 74 1 7 130 126 248 3.03e-17 84.7
MS.gene05035.t1 MTR_3g014650 30.357 168 97 2 51 207 89 247 8.18e-15 77.0
MS.gene05035.t1 MTR_7g089950 33.491 212 102 4 2 207 63 241 1.79e-19 91.3
MS.gene05035.t1 MTR_7g089950 29.670 182 118 3 119 300 70 241 5.24e-13 71.2
MS.gene05035.t1 MTR_8g095170 31.797 217 135 4 15 229 42 247 1.88e-19 90.9
MS.gene05035.t1 MTR_8g095170 36.486 148 81 2 2 137 52 198 1.33e-15 79.3
MS.gene05035.t1 MTR_8g095170 29.064 203 110 4 95 296 28 197 6.25e-12 67.8
MS.gene05035.t1 MTR_8g095170 31.797 217 135 4 15 229 42 247 2.76e-19 90.9
MS.gene05035.t1 MTR_8g095170 36.486 148 81 2 2 137 52 198 2.59e-15 78.6
MS.gene05035.t1 MTR_8g095170 29.064 203 110 4 95 296 28 197 1.40e-11 67.0
MS.gene05035.t1 MTR_4g099180 32.903 155 84 4 31 184 161 296 3.99e-12 67.4
MS.gene05035.t1 MTR_4g099180 34.014 147 77 4 31 176 161 288 1.33e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05035.t1 AT1G60890 30.000 350 209 6 69 402 18 347 1.46e-32 130
MS.gene05035.t1 AT1G60890 40.102 197 108 2 2 198 21 207 1.73e-32 130
MS.gene05035.t1 AT1G60890 30.000 350 209 6 69 402 6 335 1.60e-32 130
MS.gene05035.t1 AT1G60890 39.130 207 115 3 2 207 9 205 1.97e-32 130
MS.gene05035.t1 AT1G10900 29.262 393 183 9 9 397 16 317 6.12e-31 125
MS.gene05035.t1 AT1G10900 36.686 169 97 3 3 171 33 191 8.26e-18 86.3
MS.gene05035.t1 AT1G10900 34.091 132 63 2 1 132 77 184 4.32e-13 71.6
MS.gene05035.t1 AT1G10900 29.262 393 183 9 9 397 16 317 6.12e-31 125
MS.gene05035.t1 AT1G10900 36.686 169 97 3 3 171 33 191 8.26e-18 86.3
MS.gene05035.t1 AT1G10900 34.091 132 63 2 1 132 77 184 4.32e-13 71.6
MS.gene05035.t1 AT1G10900 29.262 393 183 9 9 397 16 317 6.12e-31 125
MS.gene05035.t1 AT1G10900 36.686 169 97 3 3 171 33 191 8.26e-18 86.3
MS.gene05035.t1 AT1G10900 34.091 132 63 2 1 132 77 184 4.32e-13 71.6
MS.gene05035.t1 AT1G21980 37.430 179 102 2 8 186 80 248 1.86e-25 109
MS.gene05035.t1 AT1G21980 31.944 216 125 6 51 260 76 275 2.98e-18 87.8
MS.gene05035.t1 AT1G21980 32.515 163 100 2 2 164 97 249 5.11e-16 80.9
MS.gene05035.t1 AT1G21980 27.136 199 112 2 94 292 73 238 7.39e-14 73.9
MS.gene05035.t1 AT1G21980 27.805 205 115 2 119 323 75 246 7.45e-14 73.9
MS.gene05035.t1 AT3G07960 33.862 189 106 3 2 181 48 226 1.13e-23 103
MS.gene05035.t1 AT3G07960 33.036 224 125 4 9 232 32 230 1.26e-22 100
MS.gene05035.t1 AT3G07960 27.234 235 137 3 94 328 24 224 4.64e-18 87.0
MS.gene05035.t1 AT3G07960 24.355 349 205 8 48 375 12 322 3.68e-14 74.7
MS.gene05035.t1 AT3G07960 33.862 189 106 3 2 181 48 226 1.13e-23 103
MS.gene05035.t1 AT3G07960 33.036 224 125 4 9 232 32 230 1.26e-22 100
MS.gene05035.t1 AT3G07960 27.234 235 137 3 94 328 24 224 4.64e-18 87.0
MS.gene05035.t1 AT3G07960 24.355 349 205 8 48 375 12 322 3.68e-14 74.7
MS.gene05035.t1 AT3G07960 33.862 189 106 3 2 181 48 226 1.37e-23 103
MS.gene05035.t1 AT3G07960 33.036 224 125 4 9 232 32 230 1.74e-22 100
MS.gene05035.t1 AT3G07960 27.234 235 137 3 94 328 24 224 5.67e-18 86.7
MS.gene05035.t1 AT3G07960 24.355 349 205 8 48 375 12 322 5.79e-14 74.3
MS.gene05035.t1 AT1G77660 33.649 211 125 3 115 320 173 373 1.72e-23 102
MS.gene05035.t1 AT1G77660 32.381 210 107 4 45 253 172 347 3.81e-18 86.3
MS.gene05035.t1 AT1G77660 37.302 126 78 1 9 134 229 353 7.54e-15 76.3
MS.gene05035.t1 AT1G77660 30.978 184 94 3 1 161 175 348 6.55e-14 73.6
MS.gene05035.t1 AT2G41210 35.829 187 110 3 9 195 75 251 2.67e-23 103
MS.gene05035.t1 AT2G41210 30.851 188 111 3 2 180 91 268 3.31e-18 87.4
MS.gene05035.t1 AT2G41210 30.049 203 118 4 95 274 68 269 1.66e-17 85.5
MS.gene05035.t1 AT2G41210 29.189 185 96 4 108 292 60 209 1.59e-11 66.6
MS.gene05035.t1 AT3G07960 33.862 189 106 3 2 181 48 226 2.87e-23 102
MS.gene05035.t1 AT3G07960 34.171 199 118 3 9 207 32 217 2.97e-22 99.8
MS.gene05035.t1 AT3G07960 27.234 235 137 3 94 328 24 224 1.42e-17 85.5
MS.gene05035.t1 AT3G07960 24.355 349 205 8 48 375 12 322 1.52e-13 73.2
MS.gene05035.t1 AT1G77740 32.394 213 110 4 8 220 81 259 4.38e-23 102
MS.gene05035.t1 AT1G77740 34.356 163 97 2 2 164 98 250 4.73e-18 87.0
MS.gene05035.t1 AT1G77740 29.741 232 122 4 51 282 77 267 2.07e-16 82.0
MS.gene05035.t1 AT1G77740 27.136 199 112 2 94 292 74 239 2.87e-12 69.3
MS.gene05035.t1 AT1G77740 32.394 213 110 4 8 220 81 259 4.38e-23 102
MS.gene05035.t1 AT1G77740 34.356 163 97 2 2 164 98 250 4.73e-18 87.0
MS.gene05035.t1 AT1G77740 29.741 232 122 4 51 282 77 267 2.07e-16 82.0
MS.gene05035.t1 AT1G77740 27.136 199 112 2 94 292 74 239 2.87e-12 69.3
MS.gene05035.t1 AT1G21920 34.555 191 115 2 115 305 169 349 4.61e-23 100
MS.gene05035.t1 AT1G21920 32.536 209 108 2 45 253 168 343 3.38e-20 92.0
MS.gene05035.t1 AT1G21920 31.522 184 93 3 1 161 171 344 7.49e-15 75.9
MS.gene05035.t1 AT1G21920 37.097 124 77 1 9 132 225 347 1.98e-14 74.7
MS.gene05035.t1 AT1G21920 29.146 199 128 3 169 357 168 363 9.46e-12 66.6
MS.gene05035.t1 AT1G21920 34.555 191 115 2 115 305 169 349 6.40e-23 100
MS.gene05035.t1 AT1G21920 33.333 210 105 4 45 253 168 343 3.77e-20 92.4
MS.gene05035.t1 AT1G21920 31.522 184 93 3 1 161 171 344 7.89e-15 76.3
MS.gene05035.t1 AT1G21920 37.097 124 77 1 9 132 225 347 2.78e-14 74.7
MS.gene05035.t1 AT1G21920 29.146 199 128 3 169 357 168 363 1.74e-11 65.9
MS.gene05035.t1 AT3G56960 33.668 199 99 3 9 207 77 242 1.09e-21 98.2
MS.gene05035.t1 AT3G56960 28.326 233 133 3 96 328 71 269 2.83e-18 87.8
MS.gene05035.t1 AT3G56960 30.319 188 112 3 2 180 93 270 5.26e-17 84.0
MS.gene05035.t1 AT3G56960 33.668 199 99 3 9 207 77 242 1.09e-21 98.2
MS.gene05035.t1 AT3G56960 28.326 233 133 3 96 328 71 269 2.83e-18 87.8
MS.gene05035.t1 AT3G56960 30.319 188 112 3 2 180 93 270 5.26e-17 84.0
MS.gene05035.t1 AT3G09920 38.000 150 90 2 144 292 68 215 6.72e-21 95.9
MS.gene05035.t1 AT3G09920 29.659 381 200 14 48 407 50 383 1.61e-20 94.7
MS.gene05035.t1 AT3G09920 31.863 204 129 2 1 204 50 243 1.62e-20 94.7
MS.gene05035.t1 AT3G09920 38.000 150 90 2 144 292 68 215 6.72e-21 95.9
MS.gene05035.t1 AT3G09920 29.659 381 200 14 48 407 50 383 1.61e-20 94.7
MS.gene05035.t1 AT3G09920 31.863 204 129 2 1 204 50 243 1.62e-20 94.7
MS.gene05035.t1 AT3G09920 38.000 150 90 2 144 292 68 215 6.72e-21 95.9
MS.gene05035.t1 AT3G09920 29.659 381 200 14 48 407 50 383 1.61e-20 94.7
MS.gene05035.t1 AT3G09920 31.863 204 129 2 1 204 50 243 1.62e-20 94.7
MS.gene05035.t1 AT3G09920 38.000 150 90 2 144 292 68 215 6.72e-21 95.9
MS.gene05035.t1 AT3G09920 29.659 381 200 14 48 407 50 383 1.61e-20 94.7
MS.gene05035.t1 AT3G09920 31.863 204 129 2 1 204 50 243 1.62e-20 94.7
MS.gene05035.t1 AT2G35170 33.871 186 113 2 3 188 225 400 7.14e-21 95.1
MS.gene05035.t1 AT2G35170 32.609 184 114 3 116 299 222 395 2.29e-18 87.4
MS.gene05035.t1 AT2G35170 31.319 182 116 1 32 213 230 402 1.97e-17 84.7
MS.gene05035.t1 AT2G35170 30.682 176 119 2 167 342 218 390 3.21e-11 65.5
MS.gene05035.t1 AT4G17080 33.871 186 113 2 3 188 256 431 1.73e-20 94.0
MS.gene05035.t1 AT4G17080 32.065 184 115 3 116 299 253 426 1.41e-19 91.3
MS.gene05035.t1 AT4G17080 29.787 188 123 1 26 213 255 433 1.88e-18 87.8
MS.gene05035.t1 AT4G17080 29.834 181 124 2 160 340 242 419 1.51e-11 66.6
MS.gene05035.t1 AT2G26420 35.294 187 88 3 8 194 57 210 5.35e-20 93.2
MS.gene05035.t1 AT2G26420 31.000 200 105 2 94 293 50 216 3.81e-15 78.2
MS.gene05035.t1 AT2G26420 26.699 206 114 3 87 292 24 192 6.89e-12 67.8
MS.gene05035.t1 AT2G26420 35.294 187 88 3 8 194 57 210 5.99e-20 92.8
MS.gene05035.t1 AT2G26420 31.000 200 105 2 94 293 50 216 4.99e-15 77.8
MS.gene05035.t1 AT2G26420 26.699 206 114 3 87 292 24 192 7.80e-12 67.8
MS.gene05035.t1 AT2G26420 35.294 187 88 3 8 194 57 210 5.99e-20 92.8
MS.gene05035.t1 AT2G26420 31.000 200 105 2 94 293 50 216 4.99e-15 77.8
MS.gene05035.t1 AT2G26420 26.699 206 114 3 87 292 24 192 7.80e-12 67.8

Find 108 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTCCATTCTTATAAGTT+TGG 0.224406 3.4:+66044269 None:intergenic
GGGCTGTCTATATCGGAATT+TGG 0.248124 3.4:-66044018 MS.gene05035:CDS
TCAATTGCGCTTCATGGATT+TGG 0.261295 3.4:-66044061 MS.gene05035:CDS
GATTCATATAATAGCTTTGA+TGG 0.263410 3.4:+66043229 None:intergenic
GGTATGTCTCCACTGGAAAT+TGG 0.298155 3.4:-66043880 MS.gene05035:CDS
ACCTCCATTCTTATAAGTTT+GGG 0.299872 3.4:+66044270 None:intergenic
AAATTGGAAAACAGGGAAAA+TGG 0.307307 3.4:-66043864 MS.gene05035:CDS
GAGTTTATAGATATGCAAAT+TGG 0.311182 3.4:-66044039 MS.gene05035:CDS
AAGTATGAGGGTGAATTCTT+TGG 0.311574 3.4:-66044316 MS.gene05035:CDS
GATACTGGTGATGTCTTCTA+TGG 0.312885 3.4:-66043956 MS.gene05035:CDS
GCTGCTGGTGATGTCTTTGA+TGG 0.325581 3.4:-66043677 MS.gene05035:CDS
CAGAGAAAGGCCTGAAGATA+TGG 0.327525 3.4:-66043493 MS.gene05035:CDS
GGGATGTCTACATTGGAAAT+TGG 0.328769 3.4:-66044114 MS.gene05035:CDS
TCCACTGGAAATTGGAAAAC+AGG 0.334764 3.4:-66043872 MS.gene05035:CDS
TATGAGGGTGATTGGGTTGA+TGG 0.335707 3.4:-66044382 MS.gene05035:CDS
GGAAAATACACACGCTCAAT+TGG 0.335840 3.4:-66043554 MS.gene05035:CDS
AAATTTGACAAAAGACAAAA+TGG 0.343438 3.4:-66043723 MS.gene05035:CDS
TTCTATGGTTGTTGGTCAAA+TGG 0.344466 3.4:-66043941 MS.gene05035:CDS
AAAGACAAAATGGATGAAAC+AGG 0.358390 3.4:-66043713 MS.gene05035:CDS
GATCAATATATGAGGGTGAT+TGG 0.359146 3.4:-66044390 MS.gene05035:CDS
TGTCGATCAATTGCGCTTCA+TGG 0.361676 3.4:-66044067 MS.gene05035:CDS
TCATTGCAGATTTCAAATAA+AGG 0.363457 3.4:-66043443 MS.gene05035:CDS
GAACAATAAACGAGGGTGAT+TGG 0.367652 3.4:-66043532 MS.gene05035:CDS
GCAAATTGGGCTGTCTATAT+CGG 0.371248 3.4:-66044025 MS.gene05035:CDS
GTGATGTCTTTGATGGTTGT+TGG 0.371599 3.4:-66043670 MS.gene05035:CDS
AATGGACTTAGACATGGATT+TGG 0.376853 3.4:-66043782 MS.gene05035:CDS
AATGGACTTATACATGGATA+TGG 0.378270 3.4:-66043644 MS.gene05035:CDS
GCTAACGAGGATGTCGATAT+TGG 0.378942 3.4:-66043608 MS.gene05035:CDS
AATGGACTTCAACATGGATA+TGG 0.393948 3.4:-66043923 MS.gene05035:CDS
GAGGTAGCTACATTGGAAAT+TGG 0.394936 3.4:-66044252 MS.gene05035:CDS
TTTGATGGTTGTTGGTCAAA+TGG 0.395785 3.4:-66043662 MS.gene05035:CDS
AACAATAAACGAGGGTGATT+GGG 0.398395 3.4:-66043531 MS.gene05035:CDS
CAAAGAAGAGATCGTGCATA+TGG 0.398435 3.4:-66043265 MS.gene05035:CDS
AGTATGAGGGTGAATTCTTT+GGG 0.399117 3.4:-66044315 MS.gene05035:CDS
GGCATCTGGTCTGAGCCCTT+TGG 0.402901 3.4:-66043411 MS.gene05035:CDS
TGGAAGAGGGATTGAGACTT+TGG 0.411239 3.4:-66044212 MS.gene05035:CDS
GGGATTATGAGTTGGGATAC+TGG 0.413810 3.4:-66043971 MS.gene05035:CDS
TTTGAAGGGCCCATATCTTC+AGG 0.413912 3.4:+66043483 None:intergenic
TTATGTGTTACTTCTGAATA+CGG 0.414297 3.4:+66043338 None:intergenic
GAGTTTCTAGATTTGCAAAT+GGG 0.424260 3.4:-66044135 MS.gene05035:CDS
TGGATCAGGGATTATGAGTT+GGG 0.431926 3.4:-66043978 MS.gene05035:CDS
GTGATGTCTTCTATGGTTGT+TGG 0.434669 3.4:-66043949 MS.gene05035:CDS
TGAGCCCTTTGGTTATCAAA+AGG 0.442205 3.4:-66043400 MS.gene05035:CDS
AGTTTATAGATATGCAAATT+GGG 0.447008 3.4:-66044038 MS.gene05035:CDS
GGAGTTTCTAGATTTGCAAA+TGG 0.447368 3.4:-66044136 MS.gene05035:CDS
TGGGTTGATGGAGATAAGAC+AGG 0.450163 3.4:-66044370 MS.gene05035:CDS
GGGATTGAGACTTTGGCTAA+TGG 0.450253 3.4:-66044205 MS.gene05035:CDS
AAAGGTAAGTCTGAGGCATC+TGG 0.454416 3.4:-66043425 MS.gene05035:CDS
ATTTGAAATCTGCAATGAAG+TGG 0.473202 3.4:+66043448 None:intergenic
TGATGATGGATCAATATATG+AGG 0.474674 3.4:-66044398 MS.gene05035:CDS
TACATTGGTAACATCAAGAA+TGG 0.480521 3.4:-66044454 MS.gene05035:CDS
CCCTCATACTTGTCTCCTGA+TGG 0.482924 3.4:+66044328 None:intergenic
GGAGTTTATAGATCCGCAAA+TGG 0.482951 3.4:-66043902 MS.gene05035:CDS
GGCACCCAAACTTATAAGAA+TGG 0.483958 3.4:-66044274 MS.gene05035:CDS
AAACAGGGAAAATGGATGTT+GGG 0.487088 3.4:-66043856 MS.gene05035:CDS
GGAGTTTATAGATTTGCAAA+TGG 0.487399 3.4:-66043761 MS.gene05035:CDS
GAGCCCTTTGGTTATCAAAA+GGG 0.495737 3.4:-66043399 MS.gene05035:CDS
AAAACAGGGAAAATGGATGT+TGG 0.499221 3.4:-66043857 MS.gene05035:CDS
AGAGAAAGGCCTGAAGATAT+GGG 0.500116 3.4:-66043492 MS.gene05035:CDS
TTCTTTGGGAATTGCCGTCA+TGG 0.500760 3.4:-66044301 MS.gene05035:CDS
GCCATCAGGAGACAAGTATG+AGG 0.502897 3.4:-66044329 MS.gene05035:CDS
TGGTCAAATGGACTTATACA+TGG 0.503975 3.4:-66043650 MS.gene05035:CDS
AAAATGAAAGGGATGGATCA+GGG 0.508482 3.4:-66043991 MS.gene05035:CDS
TGCAATGAAGTGGAACCATC+GGG 0.511793 3.4:+66043458 None:intergenic
AAGAATGGAGGTAGCTACAT+TGG 0.512285 3.4:-66044259 MS.gene05035:CDS
TGAAACAGGGATTATGAGTT+GGG 0.518447 3.4:-66043699 MS.gene05035:CDS
ATCAATATATGAGGGTGATT+GGG 0.524240 3.4:-66044389 MS.gene05035:CDS
CTGCAATGAAGTGGAACCAT+CGG 0.525925 3.4:+66043457 None:intergenic
GGGAATTGCCGTCATGGCAA+TGG 0.527145 3.4:-66044295 MS.gene05035:CDS
ATGGATCAGGGATTATGAGT+TGG 0.528428 3.4:-66043979 MS.gene05035:CDS
ATGAAACAGGGATTATGAGT+TGG 0.528516 3.4:-66043700 MS.gene05035:CDS
AATGATAAAAGAGATGGAAG+AGG 0.531418 3.4:-66044226 MS.gene05035:CDS
GCAAATGGGTATGTCTCCAC+TGG 0.533118 3.4:-66043887 MS.gene05035:CDS
AAGTACACACGGTTTGATGA+TGG 0.537447 3.4:-66044412 MS.gene05035:CDS
GCAAATGGGGATGTCTACAT+TGG 0.539662 3.4:-66044121 MS.gene05035:CDS
GGGATTATGAGTTGGGCTGC+TGG 0.543685 3.4:-66043692 MS.gene05035:CDS
AATGGACTTGCTCATGGCAA+AGG 0.546265 3.4:-66044436 MS.gene05035:CDS
AAAGAAGAGATCGTGCATAT+GGG 0.548304 3.4:-66043264 MS.gene05035:CDS
AGTGGAGACATACCCATTTG+CGG 0.548569 3.4:+66043889 None:intergenic
TTTGGGTGCCATTGCCATGA+CGG 0.549134 3.4:+66044287 None:intergenic
ATGATAAAAGAGATGGAAGA+GGG 0.554175 3.4:-66044225 MS.gene05035:CDS
ATCAAGAATGGACTTGCTCA+TGG 0.558306 3.4:-66044442 MS.gene05035:CDS
GAGTTTATAGATCCGCAAAT+GGG 0.565304 3.4:-66043901 MS.gene05035:CDS
GATGGAGATAAGACAGGGAA+AGG 0.568932 3.4:-66044364 MS.gene05035:CDS
TGGTCAAATGGACTTCAACA+TGG 0.576448 3.4:-66043929 MS.gene05035:CDS
ATCAAAAGGGAATATATGCA+AGG 0.589384 3.4:-66043386 MS.gene05035:CDS
CCACTGGAAATTGGAAAACA+GGG 0.596163 3.4:-66043871 MS.gene05035:CDS
ATGTCAAATGGACTTAGACA+TGG 0.597161 3.4:-66043788 MS.gene05035:CDS
AGTAAACTTCTCCATGACAA+TGG 0.599616 3.4:-66043575 MS.gene05035:CDS
TTCAAATAAAGGTAAGTCTG+AGG 0.603610 3.4:-66043432 MS.gene05035:CDS
GGACTGTTCATAGAGCCATC+AGG 0.607086 3.4:-66044343 MS.gene05035:CDS
TACATTGGAAATTGGACTTG+TGG 0.609300 3.4:-66044106 MS.gene05035:CDS
AGTTTCTAGATTTGCAAATG+GGG 0.609982 3.4:-66044134 MS.gene05035:CDS
GCAAATGGTGATGTCTACAT+CGG 0.612093 3.4:-66043746 MS.gene05035:CDS
TCAGACGAGGGTGTCTACAT+TGG 0.615373 3.4:-66044469 MS.gene05035:CDS
GGGTTGATGGAGATAAGACA+GGG 0.616802 3.4:-66044369 MS.gene05035:CDS
CCATCAGGAGACAAGTATGA+GGG 0.619284 3.4:-66044328 MS.gene05035:CDS
AAAGATGTTGTATTCAGACG+AGG 0.623934 3.4:-66044482 None:intergenic
AAGATGTTGTATTCAGACGA+GGG 0.637143 3.4:-66044481 MS.gene05035:CDS
TATGCAAGGAGTTCTTATTG+TGG 0.641870 3.4:-66043372 MS.gene05035:CDS
ACCCAAACTTATAAGAATGG+AGG 0.651940 3.4:-66044271 MS.gene05035:CDS
CTCAATTGGAACAATAAACG+AGG 0.654960 3.4:-66043540 MS.gene05035:CDS
GATGATGGATCAATATATGA+GGG 0.656551 3.4:-66044397 MS.gene05035:CDS
TGGGCCCTTCAAAATCCCGA+TGG 0.671812 3.4:-66043473 MS.gene05035:CDS
AAGACAAAATGGATGAAACA+GGG 0.684508 3.4:-66043712 MS.gene05035:CDS
TCAATTGGAACAATAAACGA+GGG 0.715645 3.4:-66043539 MS.gene05035:CDS
AGTTTATAGATATGCTAACG+AGG 0.727650 3.4:-66043621 MS.gene05035:CDS
ATGGCAAAGGAAAGTACACA+CGG 0.733793 3.4:-66044423 MS.gene05035:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTGGAAAAAAAATGAAA+GGG - chr3.4:66043708-66043727 MS.gene05035:CDS 15.0%
!! AAATTTGACAAAAGACAAAA+TGG - chr3.4:66043987-66044006 MS.gene05035:CDS 20.0%
!! AATAACAACAACAAAAAACA+GGG - chr3.4:66044402-66044421 MS.gene05035:CDS 20.0%
!! AGTTTATAGATATGCAAATT+GGG - chr3.4:66043672-66043691 MS.gene05035:CDS 20.0%
!! GAATTTGGAAAAAAAATGAA+AGG - chr3.4:66043707-66043726 MS.gene05035:CDS 20.0%
!! TAATAACAACAACAAAAAAC+AGG - chr3.4:66044401-66044420 MS.gene05035:CDS 20.0%
! GAGTTTATAGATATGCAAAT+TGG - chr3.4:66043671-66043690 MS.gene05035:CDS 25.0%
! TCATTGCAGATTTCAAATAA+AGG - chr3.4:66044267-66044286 MS.gene05035:CDS 25.0%
! TGGAAAAATGATAAAAGAGA+TGG - chr3.4:66043478-66043497 MS.gene05035:CDS 25.0%
!! AATGATTTTGTACATGGATA+TGG - chr3.4:66043553-66043572 MS.gene05035:CDS 25.0%
!! TTTTATCGTTGTATGTCAAA+TGG - chr3.4:66043910-66043929 MS.gene05035:CDS 25.0%
!!! TTATGTGTTACTTCTGAATA+CGG + chr3.4:66044375-66044394 None:intergenic 25.0%
AAAGACAAAATGGATGAAAC+AGG - chr3.4:66043997-66044016 MS.gene05035:CDS 30.0%
AAATTGGAAAACAGGGAAAA+TGG - chr3.4:66043846-66043865 MS.gene05035:CDS 30.0%
AAGACAAAATGGATGAAACA+GGG - chr3.4:66043998-66044017 MS.gene05035:CDS 30.0%
AATGATAAAAGAGATGGAAG+AGG - chr3.4:66043484-66043503 MS.gene05035:CDS 30.0%
ACCTCCATTCTTATAAGTTT+GGG + chr3.4:66043443-66043462 None:intergenic 30.0%
AGTTTATAGATATGCTAACG+AGG - chr3.4:66044089-66044108 MS.gene05035:CDS 30.0%
ATCAAAAGGGAATATATGCA+AGG - chr3.4:66044324-66044343 MS.gene05035:CDS 30.0%
ATCAATATATGAGGGTGATT+GGG - chr3.4:66043321-66043340 MS.gene05035:CDS 30.0%
ATGATAAAAGAGATGGAAGA+GGG - chr3.4:66043485-66043504 MS.gene05035:CDS 30.0%
GGAGTTTATAGATTTGCAAA+TGG - chr3.4:66043949-66043968 MS.gene05035:CDS 30.0%
GTTAAAAAAGTGACAGAGAA+AGG - chr3.4:66044204-66044223 MS.gene05035:CDS 30.0%
TACCTCCATTCTTATAAGTT+TGG + chr3.4:66043444-66043463 None:intergenic 30.0%
TCAATTGGAACAATAAACGA+GGG - chr3.4:66044171-66044190 MS.gene05035:CDS 30.0%
TGGAAAAAAAATGAAAGGGA+TGG - chr3.4:66043712-66043731 MS.gene05035:CDS 30.0%
TTCAAATAAAGGTAAGTCTG+AGG - chr3.4:66044278-66044297 MS.gene05035:CDS 30.0%
! AATGGACTTATACATGGATA+TGG - chr3.4:66044066-66044085 MS.gene05035:CDS 30.0%
! AGTTTCTAGATTTGCAAATG+GGG - chr3.4:66043576-66043595 MS.gene05035:CDS 30.0%
! ATTTGAAATCTGCAATGAAG+TGG + chr3.4:66044265-66044284 None:intergenic 30.0%
! GAGTTTCTAGATTTGCAAAT+GGG - chr3.4:66043575-66043594 MS.gene05035:CDS 30.0%
! TACATTGGTAACATCAAGAA+TGG - chr3.4:66043256-66043275 MS.gene05035:CDS 30.0%
! TGGTCAAATGATTTTGTACA+TGG - chr3.4:66043547-66043566 MS.gene05035:CDS 30.0%
!! ATTCCCTTTTGATAACCAAA+GGG + chr3.4:66044317-66044336 None:intergenic 30.0%
!! GATGATGGATCAATATATGA+GGG - chr3.4:66043313-66043332 MS.gene05035:CDS 30.0%
!! TATTCCCTTTTGATAACCAA+AGG + chr3.4:66044318-66044337 None:intergenic 30.0%
!! TGATGATGGATCAATATATG+AGG - chr3.4:66043312-66043331 MS.gene05035:CDS 30.0%
AAAAATGAAAGGGATGGATC+AGG - chr3.4:66043718-66043737 MS.gene05035:CDS 35.0%
AAAACAGGGAAAATGGATGT+TGG - chr3.4:66043853-66043872 MS.gene05035:CDS 35.0%
AAAATGAAAGGGATGGATCA+GGG - chr3.4:66043719-66043738 MS.gene05035:CDS 35.0%
AAACAGGGAAAATGGATGTT+GGG - chr3.4:66043854-66043873 MS.gene05035:CDS 35.0%
AAAGAAGAGATCGTGCATAT+GGG - chr3.4:66044446-66044465 MS.gene05035:CDS 35.0%
AACAATAAACGAGGGTGATT+GGG - chr3.4:66044179-66044198 MS.gene05035:CDS 35.0%
AAGTATGAGGGTGAATTCTT+TGG - chr3.4:66043394-66043413 MS.gene05035:CDS 35.0%
ACCCAAACTTATAAGAATGG+AGG - chr3.4:66043439-66043458 MS.gene05035:CDS 35.0%
AGTAAACTTCTCCATGACAA+TGG - chr3.4:66044135-66044154 MS.gene05035:CDS 35.0%
AGTATGAGGGTGAATTCTTT+GGG - chr3.4:66043395-66043414 MS.gene05035:CDS 35.0%
ATGAAACAGGGATTATGAGT+TGG - chr3.4:66044010-66044029 MS.gene05035:CDS 35.0%
CTCAATTGGAACAATAAACG+AGG - chr3.4:66044170-66044189 MS.gene05035:CDS 35.0%
GAGTTTATAGATCCGCAAAT+GGG - chr3.4:66043809-66043828 MS.gene05035:CDS 35.0%
GATCAATATATGAGGGTGAT+TGG - chr3.4:66043320-66043339 MS.gene05035:CDS 35.0%
TACATTGGAAATTGGACTTG+TGG - chr3.4:66043604-66043623 MS.gene05035:CDS 35.0%
TATGCAAGGAGTTCTTATTG+TGG - chr3.4:66044338-66044357 MS.gene05035:CDS 35.0%
TGAAACAGGGATTATGAGTT+GGG - chr3.4:66044011-66044030 MS.gene05035:CDS 35.0%
TTCTATGGTTGTTGGTCAAA+TGG - chr3.4:66043769-66043788 MS.gene05035:CDS 35.0%
! AATGGACTTAGACATGGATT+TGG - chr3.4:66043928-66043947 MS.gene05035:CDS 35.0%
! AATGGACTTCAACATGGATA+TGG - chr3.4:66043787-66043806 MS.gene05035:CDS 35.0%
! ATGTCAAATGGACTTAGACA+TGG - chr3.4:66043922-66043941 MS.gene05035:CDS 35.0%
! CAGATTTTCGATTGCACTAA+TGG - chr3.4:66043880-66043899 MS.gene05035:CDS 35.0%
! GGAGTTTCTAGATTTGCAAA+TGG - chr3.4:66043574-66043593 MS.gene05035:CDS 35.0%
! GTGTGTATTTTCCATTGTCA+TGG + chr3.4:66044149-66044168 None:intergenic 35.0%
! TGGTCAAATGGACTTATACA+TGG - chr3.4:66044060-66044079 MS.gene05035:CDS 35.0%
! TTTGATGGTTGTTGGTCAAA+TGG - chr3.4:66043535-66043554 MS.gene05035:CDS 35.0%
!! ACTTGTGGTGATGTTTTTGA+TGG - chr3.4:66043619-66043638 MS.gene05035:CDS 35.0%
!! GCTAATGGTGATGTTTTTGA+TGG - chr3.4:66043520-66043539 MS.gene05035:CDS 35.0%
!! GTGATGTTTTTGATGGTTGT+TGG - chr3.4:66043527-66043546 MS.gene05035:CDS 35.0%
AAGAATGGAGGTAGCTACAT+TGG - chr3.4:66043451-66043470 MS.gene05035:CDS 40.0%
AAGTACACACGGTTTGATGA+TGG - chr3.4:66043298-66043317 MS.gene05035:CDS 40.0%
AGAGAAAGGCCTGAAGATAT+GGG - chr3.4:66044218-66044237 MS.gene05035:CDS 40.0%
ATGGATCAGGGATTATGAGT+TGG - chr3.4:66043731-66043750 MS.gene05035:CDS 40.0%
CAAAGAAGAGATCGTGCATA+TGG - chr3.4:66044445-66044464 MS.gene05035:CDS 40.0%
CCACTGGAAATTGGAAAACA+GGG - chr3.4:66043839-66043858 MS.gene05035:CDS 40.0%
GAACAATAAACGAGGGTGAT+TGG - chr3.4:66044178-66044197 MS.gene05035:CDS 40.0%
GAGCCCTTTGGTTATCAAAA+GGG - chr3.4:66044311-66044330 MS.gene05035:CDS 40.0%
GAGGTAGCTACATTGGAAAT+TGG - chr3.4:66043458-66043477 MS.gene05035:CDS 40.0%
GCAAATTGGGCTGTCTATAT+CGG - chr3.4:66043685-66043704 MS.gene05035:CDS 40.0%
GGAAAATACACACGCTCAAT+TGG - chr3.4:66044156-66044175 MS.gene05035:CDS 40.0%
GGAGTTTATAGATCCGCAAA+TGG - chr3.4:66043808-66043827 MS.gene05035:CDS 40.0%
GGCACCCAAACTTATAAGAA+TGG - chr3.4:66043436-66043455 MS.gene05035:CDS 40.0%
GGGATGTCTACATTGGAAAT+TGG - chr3.4:66043596-66043615 MS.gene05035:CDS 40.0%
GTGATGTCTTCTATGGTTGT+TGG - chr3.4:66043761-66043780 MS.gene05035:CDS 40.0%
TCAATTGCGCTTCATGGATT+TGG - chr3.4:66043649-66043668 MS.gene05035:CDS 40.0%
TCCACTGGAAATTGGAAAAC+AGG - chr3.4:66043838-66043857 MS.gene05035:CDS 40.0%
TGAGCCCTTTGGTTATCAAA+AGG - chr3.4:66044310-66044329 MS.gene05035:CDS 40.0%
TGGATCAGGGATTATGAGTT+GGG - chr3.4:66043732-66043751 MS.gene05035:CDS 40.0%
! ATCAAGAATGGACTTGCTCA+TGG - chr3.4:66043268-66043287 MS.gene05035:CDS 40.0%
! ATGGCAAAGGAAAGTACACA+CGG - chr3.4:66043287-66043306 MS.gene05035:CDS 40.0%
! GATACTGGTGATGTCTTCTA+TGG - chr3.4:66043754-66043773 MS.gene05035:CDS 40.0%
! GCAAATGGTGATGTCTACAT+CGG - chr3.4:66043964-66043983 MS.gene05035:CDS 40.0%
! GTGATGTCTTTGATGGTTGT+TGG - chr3.4:66044040-66044059 MS.gene05035:CDS 40.0%
! TGGTCAAATGGACTTCAACA+TGG - chr3.4:66043781-66043800 MS.gene05035:CDS 40.0%
AGTGGAGACATACCCATTTG+CGG + chr3.4:66043824-66043843 None:intergenic 45.0%
CAGAGAAAGGCCTGAAGATA+TGG - chr3.4:66044217-66044236 MS.gene05035:CDS 45.0%
CCATCAGGAGACAAGTATGA+GGG - chr3.4:66043382-66043401 MS.gene05035:CDS 45.0%
CTGCAATGAAGTGGAACCAT+CGG + chr3.4:66044256-66044275 None:intergenic 45.0%
GATGGAGATAAGACAGGGAA+AGG - chr3.4:66043346-66043365 MS.gene05035:CDS 45.0%
GCAAATGGGGATGTCTACAT+TGG - chr3.4:66043589-66043608 MS.gene05035:CDS 45.0%
GCTAACGAGGATGTCGATAT+TGG - chr3.4:66044102-66044121 MS.gene05035:CDS 45.0%
GGGATTGAGACTTTGGCTAA+TGG - chr3.4:66043505-66043524 MS.gene05035:CDS 45.0%
GGGCTGTCTATATCGGAATT+TGG - chr3.4:66043692-66043711 MS.gene05035:CDS 45.0%
GGTATGTCTCCACTGGAAAT+TGG - chr3.4:66043830-66043849 MS.gene05035:CDS 45.0%
TGCAATGAAGTGGAACCATC+GGG + chr3.4:66044255-66044274 None:intergenic 45.0%
TGGAAGAGGGATTGAGACTT+TGG - chr3.4:66043498-66043517 MS.gene05035:CDS 45.0%
TGTCGATCAATTGCGCTTCA+TGG - chr3.4:66043643-66043662 MS.gene05035:CDS 45.0%
TTTGAAGGGCCCATATCTTC+AGG + chr3.4:66044230-66044249 None:intergenic 45.0%
! AAAGGTAAGTCTGAGGCATC+TGG - chr3.4:66044285-66044304 MS.gene05035:CDS 45.0%
! AATGGACTTGCTCATGGCAA+AGG - chr3.4:66043274-66043293 MS.gene05035:CDS 45.0%
! CCCTGTTTTCCAATTTCCAG+TGG + chr3.4:66043842-66043861 None:intergenic 45.0%
! GGAACCATCGGGATTTTGAA+GGG + chr3.4:66044244-66044263 None:intergenic 45.0%
! GGGATTATGAGTTGGGATAC+TGG - chr3.4:66043739-66043758 MS.gene05035:CDS 45.0%
! TGGAACCATCGGGATTTTGA+AGG + chr3.4:66044245-66044264 None:intergenic 45.0%
! TTCTTTGGGAATTGCCGTCA+TGG - chr3.4:66043409-66043428 MS.gene05035:CDS 45.0%
!! GGGTTGATGGAGATAAGACA+GGG - chr3.4:66043341-66043360 MS.gene05035:CDS 45.0%
!! TATGAGGGTGATTGGGTTGA+TGG - chr3.4:66043328-66043347 MS.gene05035:CDS 45.0%
!! TGGGTTGATGGAGATAAGAC+AGG - chr3.4:66043340-66043359 MS.gene05035:CDS 45.0%
CCCTCATACTTGTCTCCTGA+TGG + chr3.4:66043385-66043404 None:intergenic 50.0%
GCAAATGGGTATGTCTCCAC+TGG - chr3.4:66043823-66043842 MS.gene05035:CDS 50.0%
GCCATCAGGAGACAAGTATG+AGG - chr3.4:66043381-66043400 MS.gene05035:CDS 50.0%
GGACTGTTCATAGAGCCATC+AGG - chr3.4:66043367-66043386 MS.gene05035:CDS 50.0%
! GCTGCTGGTGATGTCTTTGA+TGG - chr3.4:66044033-66044052 MS.gene05035:CDS 50.0%
!! TCAGACGAGGGTGTCTACAT+TGG - chr3.4:66043241-66043260 MS.gene05035:CDS 50.0%
!! TTTGGGTGCCATTGCCATGA+CGG + chr3.4:66043426-66043445 None:intergenic 50.0%
GGGAATTGCCGTCATGGCAA+TGG - chr3.4:66043415-66043434 MS.gene05035:CDS 55.0%
TGGGCCCTTCAAAATCCCGA+TGG - chr3.4:66044237-66044256 MS.gene05035:CDS 55.0%
! GGGATTATGAGTTGGGCTGC+TGG - chr3.4:66044018-66044037 MS.gene05035:CDS 55.0%
GGCATCTGGTCTGAGCCCTT+TGG - chr3.4:66044299-66044318 MS.gene05035:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 66043232 66044500 66043232 ID=MS.gene05035
chr3.4 mRNA 66043232 66044500 66043232 ID=MS.gene05035.t1;Parent=MS.gene05035
chr3.4 exon 66043232 66044500 66043232 ID=MS.gene05035.t1.exon1;Parent=MS.gene05035.t1
chr3.4 CDS 66043232 66044500 66043232 ID=cds.MS.gene05035.t1;Parent=MS.gene05035.t1
Gene Sequence

>MS.gene05035

ATGTTGTATTCAGACGAGGGTGTCTACATTGGTAACATCAAGAATGGACTTGCTCATGGCAAAGGAAAGTACACACGGTTTGATGATGGATCAATATATGAGGGTGATTGGGTTGATGGAGATAAGACAGGGAAAGGACTGTTCATAGAGCCATCAGGAGACAAGTATGAGGGTGAATTCTTTGGGAATTGCCGTCATGGCAATGGCACCCAAACTTATAAGAATGGAGGTAGCTACATTGGAAATTGGAAAAATGATAAAAGAGATGGAAGAGGGATTGAGACTTTGGCTAATGGTGATGTTTTTGATGGTTGTTGGTCAAATGATTTTGTACATGGATATGGAGTTTCTAGATTTGCAAATGGGGATGTCTACATTGGAAATTGGACTTGTGGTGATGTTTTTGATGGTTGTCGATCAATTGCGCTTCATGGATTTGGAGTTTATAGATATGCAAATTGGGCTGTCTATATCGGAATTTGGAAAAAAAATGAAAGGGATGGATCAGGGATTATGAGTTGGGATACTGGTGATGTCTTCTATGGTTGTTGGTCAAATGGACTTCAACATGGATATGGAGTTTATAGATCCGCAAATGGGTATGTCTCCACTGGAAATTGGAAAACAGGGAAAATGGATGTTGGGAGACAGATTTTCGATTGCACTAATGGTGATGTTTTTTATCGTTGTATGTCAAATGGACTTAGACATGGATTTGGAGTTTATAGATTTGCAAATGGTGATGTCTACATCGGAAATTTGACAAAAGACAAAATGGATGAAACAGGGATTATGAGTTGGGCTGCTGGTGATGTCTTTGATGGTTGTTGGTCAAATGGACTTATACATGGATATGGAGTTTATAGATATGCTAACGAGGATGTCGATATTGGTAACTTCATGAGTAAACTTCTCCATGACAATGGAAAATACACACGCTCAATTGGAACAATAAACGAGGGTGATTGGGTTGTTAAAAAAGTGACAGAGAAAGGCCTGAAGATATGGGCCCTTCAAAATCCCGATGGTTCCACTTCATTGCAGATTTCAAATAAAGGTAAGTCTGAGGCATCTGGTCTGAGCCCTTTGGTTATCAAAAGGGAATATATGCAAGGAGTTCTTATTGTGGAGAAAATCAGACCGTATTCAGAAGTAACACATAATAATAATAATAACAACAACAAAAAACAGGGTGCATTTAGTGCGAAGAAAGCAAAGAAGAGATCGTGCATATGGGCAATTTTGAAGACCATCAAAGCTATTATATGA
Protein sequence

>MS.gene05035.t1

MLYSDEGVYIGNIKNGLAHGKGKYTRFDDGSIYEGDWVDGDKTGKGLFIEPSGDKYEGEFFGNCRHGNGTQTYKNGGSYIGNWKNDKRDGRGIETLANGDVFDGCWSNDFVHGYGVSRFANGDVYIGNWTCGDVFDGCRSIALHGFGVYRYANWAVYIGIWKKNERDGSGIMSWDTGDVFYGCWSNGLQHGYGVYRSANGYVSTGNWKTGKMDVGRQIFDCTNGDVFYRCMSNGLRHGFGVYRFANGDVYIGNLTKDKMDETGIMSWAAGDVFDGCWSNGLIHGYGVYRYANEDVDIGNFMSKLLHDNGKYTRSIGTINEGDWVVKKVTEKGLKIWALQNPDGSTSLQISNKGKSEASGLSPLVIKREYMQGVLIVEKIRPYSEVTHNNNNNNNKKQGAFSAKKAKKRSCIWAILKTIKAII