AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25981


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25981.t1 MTR_3g077500 99.187 123 1 0 1 123 254 376 8.24e-83 247
MS.gene25981.t1 MTR_3g111540 38.393 112 69 0 6 117 236 347 1.65e-23 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25981.t1 AT5G63040 84.821 112 17 0 1 112 245 356 3.68e-56 178
MS.gene25981.t1 AT5G63040 84.821 112 17 0 1 112 245 356 5.42e-56 178
MS.gene25981.t1 AT1G48460 41.071 112 66 0 6 117 185 296 1.25e-22 90.1
MS.gene25981.t1 AT1G48460 41.071 112 66 0 6 117 222 333 2.11e-22 90.1

Find 42 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTTACTTGAAAGATTAA+TGG 0.186826 3.3:-65764473 None:intergenic
GCTTTATCATTCACAGTTAG+AGG 0.284069 3.3:+65765062 MS.gene25981:CDS
CATACTGCTCAAATGCAAAT+TGG 0.318732 3.3:-65764415 None:intergenic
CATGGCTATAAACAGCTCTT+TGG 0.321164 3.3:+65765106 MS.gene25981:CDS
GGAATTGTAGGACATTTAGA+AGG 0.334567 3.3:-65765133 None:intergenic
TGTAATTCCCATGGTCACTA+TGG 0.335205 3.3:+65764322 None:intergenic
TCCCATGGTCACTATGGGTT+TGG 0.348807 3.3:+65764328 None:intergenic
CGCAACTCTTGCTCCGTACC+TGG 0.368258 3.3:+65764379 MS.gene25981:CDS
GCAAGAGTTGCGGAGGCTGC+GGG 0.391097 3.3:-65764368 None:intergenic
TCCTTGGTGTGATTTGCATC+TGG 0.400119 3.3:+65765153 MS.gene25981:CDS
ACTAAATAGAGCAGCACAAC+TGG 0.402095 3.3:+65765034 MS.gene25981:CDS
ATTCTGGACTTCACTGCATC+GGG 0.403109 3.3:-65765220 None:intergenic
ATCTCATGAGGAAGCTTGAC+AGG 0.413139 3.3:-65765178 None:intergenic
GTTGTTATTTACAGGTGTAT+AGG 0.423319 3.3:+65765003 MS.gene25981:intron
AGCAAGAGTTGCGGAGGCTG+CGG 0.424504 3.3:-65764369 None:intergenic
GGTTTGGTCTGGCCATGGAC+TGG 0.426393 3.3:+65764344 MS.gene25981:CDS
GTTGTGCTGCTCTATTTAGT+TGG 0.426838 3.3:-65765031 None:intergenic
ATGGCTATAAACAGCTCTTT+GGG 0.429410 3.3:+65765107 MS.gene25981:CDS
GATATCGGAAGTCACCTTCA+TGG 0.441603 3.3:+65764441 MS.gene25981:CDS
CTATGGGTTTGGTCTGGCCA+TGG 0.445695 3.3:+65764339 MS.gene25981:CDS
GTAATTCCCATGGTCACTAT+GGG 0.460084 3.3:+65764323 None:intergenic
CTAAATGTCCTACAATTCCT+TGG 0.466535 3.3:+65765137 MS.gene25981:CDS
GAGGCTGCGGGGCCAGTCCA+TGG 0.473216 3.3:-65764356 None:intergenic
AAATCACACCAAGGAATTGT+AGG 0.479343 3.3:-65765145 None:intergenic
TTGAGCAGTATGCAAGATAT+CGG 0.484764 3.3:+65764426 MS.gene25981:CDS
TGGTCACTATGGGTTTGGTC+TGG 0.490074 3.3:+65764333 MS.gene25981:CDS
AATTCTGGACTTCACTGCAT+CGG 0.521546 3.3:-65765221 None:intergenic
GACCAAACCCATAGTGACCA+TGG 0.592987 3.3:-65764330 None:intergenic
ACTCTTGCTCCGTACCTGGT+TGG 0.596249 3.3:+65764383 MS.gene25981:CDS
AATGGAATTGCAGACCATGA+AGG 0.608122 3.3:-65764455 None:intergenic
ACAACTATACCAACCAGGTA+CGG 0.611265 3.3:-65764392 None:intergenic
GAATTGTAGGACATTTAGAA+GGG 0.618173 3.3:-65765132 None:intergenic
CAAGAGTTGCGGAGGCTGCG+GGG 0.618410 3.3:-65764367 None:intergenic
GTACGGAGCAAGAGTTGCGG+AGG 0.624407 3.3:-65764375 None:intergenic
ACCAAACCCATAGTGACCAT+GGG 0.631445 3.3:-65764329 None:intergenic
TCTCATGAGGAAGCTTGACA+GGG 0.637541 3.3:-65765177 None:intergenic
TGAGTTGACTTCACATAACA+TGG 0.642393 3.3:+65765088 MS.gene25981:CDS
CAGAAGGTATAAATCTCATG+AGG 0.642726 3.3:-65765190 None:intergenic
TGCATCGGGTTTGAAGCAGA+AGG 0.650671 3.3:-65765206 None:intergenic
ATTGGACAACTATACCAACC+AGG 0.701067 3.3:-65764397 None:intergenic
CAGGTACGGAGCAAGAGTTG+CGG 0.709613 3.3:-65764378 None:intergenic
ACCAGATGCAAATCACACCA+AGG 0.715295 3.3:-65765154 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATCCTTATTAACTAT+TGG + chr3.3:65764626-65764645 MS.gene25981:intron 15.0%
!! AATAGTTAATAAGGATAATT+TGG - chr3.3:65764627-65764646 None:intergenic 15.0%
!! GATTATCAAAATATGAATTT+AGG - chr3.3:65764849-65764868 None:intergenic 15.0%
!!! CATTTTGAAACAATTTTTTT+TGG + chr3.3:65764753-65764772 MS.gene25981:intron 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG + chr3.3:65764650-65764669 MS.gene25981:intron 15.0%
!! AAACTAACCAATAGTTAATA+AGG - chr3.3:65764636-65764655 None:intergenic 20.0%
!! AATGATTAAAAGAAAGAGAA+TGG + chr3.3:65764593-65764612 MS.gene25981:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + chr3.3:65764713-65764732 MS.gene25981:intron 20.0%
!!! CAAATTAAGTTGTTATTTAC+AGG + chr3.3:65764995-65765014 MS.gene25981:intron 20.0%
! GTTCTTACTTGAAAGATTAA+TGG - chr3.3:65764476-65764495 None:intergenic 25.0%
!! TTTATCCAGTTTTACCTAAT+TGG + chr3.3:65764820-65764839 MS.gene25981:intron 25.0%
!!! ACAATTTTTTTTGGCTAAAG+CGG + chr3.3:65764762-65764781 MS.gene25981:intron 25.0%
ATGAATTTAGGCAACCAATT+AGG - chr3.3:65764837-65764856 None:intergenic 30.0%
GAATTGTAGGACATTTAGAA+GGG - chr3.3:65765135-65765154 None:intergenic 30.0%
GTATATTCATTCATTTCACC+AGG - chr3.3:65764516-65764535 None:intergenic 30.0%
!! AAACGACACTCATTTTGAAA+CGG - chr3.3:65764544-65764563 None:intergenic 30.0%
!! CTTAGTTCTCTATCTTTTCT+TGG + chr3.3:65764900-65764919 MS.gene25981:intron 30.0%
!! GTTGTTATTTACAGGTGTAT+AGG + chr3.3:65765003-65765022 MS.gene25981:intron 30.0%
!! TCTTTTCTTGGATTGATTCT+CGG + chr3.3:65764912-65764931 MS.gene25981:intron 30.0%
AAATCACACCAAGGAATTGT+AGG - chr3.3:65765148-65765167 None:intergenic 35.0%
ATGGCTATAAACAGCTCTTT+GGG + chr3.3:65765107-65765126 MS.gene25981:CDS 35.0%
CATACTGCTCAAATGCAAAT+TGG - chr3.3:65764418-65764437 None:intergenic 35.0%
CTAAATGTCCTACAATTCCT+TGG + chr3.3:65765137-65765156 MS.gene25981:CDS 35.0%
GCTTTATCATTCACAGTTAG+AGG + chr3.3:65765062-65765081 MS.gene25981:CDS 35.0%
GGAATTGTAGGACATTTAGA+AGG - chr3.3:65765136-65765155 None:intergenic 35.0%
TTGAGCAGTATGCAAGATAT+CGG + chr3.3:65764426-65764445 MS.gene25981:CDS 35.0%
! CAGAAGGTATAAATCTCATG+AGG - chr3.3:65765193-65765212 None:intergenic 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG + chr3.3:65764675-65764694 MS.gene25981:intron 35.0%
! TGAGTTGACTTCACATAACA+TGG + chr3.3:65765088-65765107 MS.gene25981:CDS 35.0%
! TGTGCAGAGTATTAATTGGA+GGG + chr3.3:65764679-65764698 MS.gene25981:intron 35.0%
!!! AACGACACTCATTTTGAAAC+GGG - chr3.3:65764543-65764562 None:intergenic 35.0%
AATGGAATTGCAGACCATGA+AGG - chr3.3:65764458-65764477 None:intergenic 40.0%
ACAACTATACCAACCAGGTA+CGG - chr3.3:65764395-65764414 None:intergenic 40.0%
ACTAAATAGAGCAGCACAAC+TGG + chr3.3:65765034-65765053 MS.gene25981:CDS 40.0%
ATTGGACAACTATACCAACC+AGG - chr3.3:65764400-65764419 None:intergenic 40.0%
CATGGCTATAAACAGCTCTT+TGG + chr3.3:65765106-65765125 MS.gene25981:CDS 40.0%
GGCAACCAATTAGGTAAAAC+TGG - chr3.3:65764828-65764847 None:intergenic 40.0%
TTCATTTCACCAGGAAACTG+AGG - chr3.3:65764507-65764526 None:intergenic 40.0%
! GTGTGCAGAGTATTAATTGG+AGG + chr3.3:65764678-65764697 MS.gene25981:intron 40.0%
! GTTGTGCTGCTCTATTTAGT+TGG - chr3.3:65765034-65765053 None:intergenic 40.0%
!! AAGCGGCACTCATTTTGAAA+CGG + chr3.3:65764779-65764798 MS.gene25981:intron 40.0%
!!! ACGACACTCATTTTGAAACG+GGG - chr3.3:65764542-65764561 None:intergenic 40.0%
ACCAGATGCAAATCACACCA+AGG - chr3.3:65765157-65765176 None:intergenic 45.0%
ATCTCATGAGGAAGCTTGAC+AGG - chr3.3:65765181-65765200 None:intergenic 45.0%
CAATCCAATCCTCAGTTTCC+TGG + chr3.3:65764495-65764514 MS.gene25981:intron 45.0%
GATATCGGAAGTCACCTTCA+TGG + chr3.3:65764441-65764460 MS.gene25981:CDS 45.0%
TCTCATGAGGAAGCTTGACA+GGG - chr3.3:65765180-65765199 None:intergenic 45.0%
TTCACCAGGAAACTGAGGAT+TGG - chr3.3:65764502-65764521 None:intergenic 45.0%
! TCCTTGGTGTGATTTGCATC+TGG + chr3.3:65765153-65765172 MS.gene25981:CDS 45.0%
!!! CGACACTCATTTTGAAACGG+GGG - chr3.3:65764541-65764560 None:intergenic 45.0%
!!! GACACTCATTTTGAAACGGG+GGG - chr3.3:65764540-65764559 None:intergenic 45.0%
!!! GGCACTCATTTTGAAACGGA+GGG + chr3.3:65764783-65764802 MS.gene25981:intron 45.0%
! TGCATCGGGTTTGAAGCAGA+AGG - chr3.3:65765209-65765228 None:intergenic 50.0%
! TGGTCACTATGGGTTTGGTC+TGG + chr3.3:65764333-65764352 MS.gene25981:CDS 50.0%
!!! CGGCACTCATTTTGAAACGG+AGG + chr3.3:65764782-65764801 MS.gene25981:intron 50.0%
ACTCTTGCTCCGTACCTGGT+TGG + chr3.3:65764383-65764402 MS.gene25981:CDS 55.0%
CAGGTACGGAGCAAGAGTTG+CGG - chr3.3:65764381-65764400 None:intergenic 55.0%
! CTATGGGTTTGGTCTGGCCA+TGG + chr3.3:65764339-65764358 MS.gene25981:CDS 55.0%
AGCAAGAGTTGCGGAGGCTG+CGG - chr3.3:65764372-65764391 None:intergenic 60.0%
CGCAACTCTTGCTCCGTACC+TGG + chr3.3:65764379-65764398 MS.gene25981:CDS 60.0%
GTACGGAGCAAGAGTTGCGG+AGG - chr3.3:65764378-65764397 None:intergenic 60.0%
! GGTTTGGTCTGGCCATGGAC+TGG + chr3.3:65764344-65764363 MS.gene25981:CDS 60.0%
CAAGAGTTGCGGAGGCTGCG+GGG - chr3.3:65764370-65764389 None:intergenic 65.0%
GCAAGAGTTGCGGAGGCTGC+GGG - chr3.3:65764371-65764390 None:intergenic 65.0%
GAGGCTGCGGGGCCAGTCCA+TGG - chr3.3:65764359-65764378 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 65764332 65765232 65764332 ID=MS.gene25981
chr3.3 mRNA 65764332 65765232 65764332 ID=MS.gene25981.t1;Parent=MS.gene25981
chr3.3 exon 65764332 65764487 65764332 ID=MS.gene25981.t1.exon1;Parent=MS.gene25981.t1
chr3.3 CDS 65764332 65764487 65764332 ID=cds.MS.gene25981.t1;Parent=MS.gene25981.t1
chr3.3 exon 65765017 65765232 65765017 ID=MS.gene25981.t1.exon2;Parent=MS.gene25981.t1
chr3.3 CDS 65765017 65765232 65765017 ID=cds.MS.gene25981.t1;Parent=MS.gene25981.t1
Gene Sequence

>MS.gene25981

ATGGTCACTATGGGTTTGGTCTGGCCATGGACTGGCCCCGCAGCCTCCGCAACTCTTGCTCCGTACCTGGTTGGTATAGTTGTCCAATTTGCATTTGAGCAGTATGCAAGATATCGGAAGTCACCTTCATGGTCTGCAATTCCATTAATCTTTCAAGTAAGAACAATCCAATCCTCAGTTTCCTGGTGAAATGAATGAATATACTCCCCCCGTTTCAAAATGAGTGTCGTTTTAAGTGTTTTCACACATATTAAGAAATGCAATGATTAAAAGAAAGAGAATGGTTATTTTACCAAATTATCCTTATTAACTATTGGTTAGTTTTAAAAGTAATACTTTGGAAGTAGTGTGCAGAGTATTAATTGGAGGGTACAATTGAAAAGAAAAAGTTATTATTGCATTGGAAACTGAAAGTGACATTCATTTTGAAACAATTTTTTTTGGCTAAAGCGGCACTCATTTTGAAACGGAGGGAGTAATAAAGTAGATTTATCCAGTTTTACCTAATTGGTTGCCTAAATTCATATTTTGATAATCTGAGCTTATAAATTGTTATATTTTAATTCTGCTTAGTTCTCTATCTTTTCTTGGATTGATTCTCGGATTGAATAAACTAAAACATTTCAAATACTTCTGTGATAACTTTTTTCAAATTTCATATAACAAATTAAGTTGTTATTTACAGGTGTATAGGCTGCACCAACTAAATAGAGCAGCACAACTGGTGACAGCTTTATCATTCACAGTTAGAGGAGCTGAGTTGACTTCACATAACATGGCTATAAACAGCTCTTTGGGTACCCTTCTAAATGTCCTACAATTCCTTGGTGTGATTTGCATCTGGTCCCTGTCAAGCTTCCTCATGAGATTTATACCTTCTGCTTCAAACCCGATGCAGTGA

Protein sequence

>MS.gene25981.t1

MVTMGLVWPWTGPAASATLAPYLVGIVVQFAFEQYARYRKSPSWSAIPLIFQVYRLHQLNRAAQLVTALSFTVRGAELTSHNMAINSSLGTLLNVLQFLGVICIWSLSSFLMRFIPSASNPMQ