AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42653


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42653.t1 AT2G32070 78.030 264 57 1 10 272 6 269 8.23e-155 432
MS.gene42653.t1 AT1G80780 77.567 263 59 0 10 272 6 268 6.29e-154 430
MS.gene42653.t1 AT1G80780 77.567 263 59 0 10 272 6 268 6.29e-154 430
MS.gene42653.t1 AT1G80780 77.567 263 59 0 10 272 6 268 6.67e-154 431
MS.gene42653.t1 AT5G10960 76.806 263 61 0 10 272 6 268 6.14e-153 428
MS.gene42653.t1 AT1G15920 61.091 275 101 1 1 269 1 275 9.35e-120 344
MS.gene42653.t1 AT1G15920 61.091 275 101 1 1 269 1 275 9.35e-120 344
MS.gene42653.t1 AT3G44260 53.846 273 118 7 6 271 8 279 2.09e-92 275
MS.gene42653.t1 AT5G22250 52.985 268 118 6 12 271 10 277 4.12e-89 266
MS.gene42653.t1 AT1G06450 36.680 259 146 7 17 270 10 255 2.38e-45 156
MS.gene42653.t1 AT1G61470 35.743 249 140 6 18 260 4 238 3.56e-37 133
MS.gene42653.t1 AT3G44240 37.719 228 128 6 30 254 3 219 9.04e-36 128
MS.gene42653.t1 AT1G27820 32.806 253 154 6 18 265 8 249 4.39e-31 117
MS.gene42653.t1 AT1G27890 33.468 248 149 6 18 260 3 239 1.80e-28 110

Find 0 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATACTTATCAACAATTTCA+CGG - 24280:13086-13105 MS.gene42653:intergenic 20.0%
! TGTTTGATATCATACACAAT+CGG - 24280:13588-13607 MS.gene42653:intergenic 25.0%
!! GTTGAATGATAATGTTCATT+GGG + 24280:13455-13474 MS.gene42653:CDS 25.0%
!! TGTTGAATGATAATGTTCAT+TGG + 24280:13454-13473 MS.gene42653:CDS 25.0%
!!! TTAACTTTTTCTGATGAGAA+TGG + 24280:13237-13256 MS.gene42653:CDS 25.0%
!!! TTTTAACTATCAGACTTTGA+AGG + 24280:13179-13198 MS.gene42653:CDS 25.0%
AAATATTCTGGTGTGTTGTA+TGG + 24280:13771-13790 MS.gene42653:CDS 30.0%
ATATGCTTAAAATTCCCAAC+AGG - 24280:13153-13172 MS.gene42653:intergenic 30.0%
ATTATCATTCAACACAATCC+CGG - 24280:13448-13467 MS.gene42653:intergenic 30.0%
GTTTGATATCATACACAATC+GGG - 24280:13587-13606 MS.gene42653:intergenic 30.0%
TAAGTATAACTATGTTGCGA+TGG + 24280:13098-13117 MS.gene42653:CDS 30.0%
TCTTAAAATCAATTCCGCAT+TGG - 24280:13371-13390 MS.gene42653:intergenic 30.0%
TTAACGAAGGATTTACAGAA+AGG + 24280:13000-13019 MS.gene42653:CDS 30.0%
TTGTACATTTAGGAAGTTGA+GGG + 24280:13722-13741 MS.gene42653:CDS 30.0%
! TTTCACAGTGGTTATGATTT+TGG + 24280:13483-13502 MS.gene42653:CDS 30.0%
AAAAATCCAGCTTGTGTATC+CGG - 24280:13543-13562 MS.gene42652:intergenic 35.0%
AAATTGAATTGCCAGATGCA+AGG - 24280:13291-13310 MS.gene42653:intergenic 35.0%
AAGTTCTGTAACAGTCTTCA+TGG + 24280:13615-13634 MS.gene42653:CDS 35.0%
AATGGAAATTTACCTACCTG+TGG + 24280:13255-13274 MS.gene42653:CDS 35.0%
AGATTCGATTCAAATCAGAG+AGG + 24280:13023-13042 MS.gene42653:CDS 35.0%
CTTGTACATTTAGGAAGTTG+AGG + 24280:13721-13740 MS.gene42653:CDS 35.0%
TACTCACAGCTTGTACATTT+AGG + 24280:13712-13731 MS.gene42653:CDS 35.0%
TTGAGGGAAACATTCTTCAA+CGG + 24280:13738-13757 MS.gene42653:CDS 35.0%
! ATGTTGAAGCTGATTCAGTT+AGG + 24280:13213-13232 MS.gene42653:CDS 35.0%
!! CTGGATTTTTCGATTTGATC+GGG + 24280:13553-13572 MS.gene42653:CDS 35.0%
!! GCTGGATTTTTCGATTTGAT+CGG + 24280:13552-13571 MS.gene42653:CDS 35.0%
ATGGAACGATAATCTGGAAG+AGG + 24280:13047-13066 MS.gene42653:CDS 40.0%
CGATTCAAATCAGAGAGGTA+TGG + 24280:13028-13047 MS.gene42653:CDS 40.0%
GGCGAAACAGAGAAATATTC+TGG + 24280:13759-13778 MS.gene42653:CDS 40.0%
TCTGGTGTGTTGTATGGATT+AGG + 24280:13777-13796 MS.gene42653:CDS 40.0%
TTCTGTAACAGTCTTCATGG+TGG + 24280:13618-13637 MS.gene42653:CDS 40.0%
TTTAAGAAGAACAGCGAGCA+AGG + 24280:13384-13403 MS.gene42653:CDS 40.0%
! AGAGGTATGGAACGATAATC+TGG + 24280:13041-13060 MS.gene42653:CDS 40.0%
!! AGTTTCCTGGTGTTGTTCTT+CGG + 24280:13127-13146 MS.gene42653:CDS 40.0%
!! TTTGAAGCTTTTGACGTGCA+GGG + 24280:13512-13531 MS.gene42653:CDS 40.0%
!!! CATTGGGTTACTTTTCACAG+TGG + 24280:13471-13490 MS.gene42653:CDS 40.0%
!!! TTTTGAAGCTTTTGACGTGC+AGG + 24280:13511-13530 MS.gene42653:CDS 40.0%
AACTATCCGATCCAGCTTGA+TGG - 24280:13692-13711 MS.gene42653:intergenic 45.0%
GAGAACTTCTGATGTCATCC+GGG + 24280:13427-13446 MS.gene42653:CDS 45.0%
GGAATCGACGTGAACAGATT+TGG + 24280:13405-13424 MS.gene42653:CDS 45.0%
GGAATTGCTGGAAGTTGAGA+GGG + 24280:13656-13675 MS.gene42653:CDS 45.0%
GGAGAACTTCTGATGTCATC+CGG + 24280:13426-13445 MS.gene42653:CDS 45.0%
TGGTGGTTTGAACAAGCTTG+CGG + 24280:13635-13654 MS.gene42653:CDS 45.0%
CGGAATTGCTGGAAGTTGAG+AGG + 24280:13655-13674 MS.gene42653:CDS 50.0%
GAACAAGCTTGCGGAATTGC+TGG + 24280:13644-13663 MS.gene42653:CDS 50.0%
GCGATGGATACAGAGTTTCC+TGG + 24280:13114-13133 MS.gene42653:CDS 50.0%
TGTGTGCCATCAAGCTGGAT+CGG + 24280:13683-13702 MS.gene42653:CDS 50.0%
TTGCTGGAAGTTGAGAGGGT+TGG + 24280:13660-13679 MS.gene42653:CDS 50.0%
! ATCGAGCTTTTGCGCCAATG+CGG + 24280:13354-13373 MS.gene42653:CDS 50.0%
! GCACTGATAGTCCTTGCATC+TGG + 24280:13277-13296 MS.gene42653:CDS 50.0%
! GTGTTGTTCTTCGGCCTGTT+GGG + 24280:13136-13155 MS.gene42653:CDS 50.0%
ACAGGCCGAAGAACAACACC+AGG - 24280:13135-13154 MS.gene42653:intergenic 55.0%
GCAAGGACTATCAGTGCCAC+AGG - 24280:13274-13293 MS.gene42653:intergenic 55.0%
GCTTTGCCGGATACACAAGC+TGG + 24280:13534-13553 MS.gene42653:CDS 55.0%
GGACTATCAGTGCCACAGGT+AGG - 24280:13270-13289 MS.gene42653:intergenic 55.0%
GTTGGTGTGTGCCATCAAGC+TGG + 24280:13678-13697 MS.gene42653:CDS 55.0%
TTTGACGTGCAGGGCTTTGC+CGG + 24280:13521-13540 MS.gene42653:CDS 55.0%
!! GGTGTTGTTCTTCGGCCTGT+TGG + 24280:13135-13154 MS.gene42653:CDS 55.0%


Chromosome Type Strat End Strand Name
24280 gene 12991 13818 12991 ID=MS.gene42653
24280 mRNA 12991 13818 12991 ID=MS.gene42653.t1;Parent=MS.gene42653
24280 exon 12991 13818 12991 ID=MS.gene42653.t1.exon1;Parent=MS.gene42653.t1
24280 CDS 12991 13818 12991 ID=cds.MS.gene42653.t1;Parent=MS.gene42653.t1
Gene Sequence

>MS.gene42653

ATGACGGATTTAACGAAGGATTTACAGAAAGGAGATTCGATTCAAATCAGAGAGGTATGGAACGATAATCTGGAAGAGGAATTTGTGTTGATCCGTGAAATTGTTGATAAGTATAACTATGTTGCGATGGATACAGAGTTTCCTGGTGTTGTTCTTCGGCCTGTTGGGAATTTTAAGCATATTAACGATTTTAACTATCAGACTTTGAAGGATAACGTTGATATGTTGAAGCTGATTCAGTTAGGTTTAACTTTTTCTGATGAGAATGGAAATTTACCTACCTGTGGCACTGATAGTCCTTGCATCTGGCAATTCAATTTTCGCGAGTTTAATGTTAGCGAAGATATTTTCGCTGCTGATTCGATCGAGCTTTTGCGCCAATGCGGAATTGATTTTAAGAAGAACAGCGAGCAAGGAATCGACGTGAACAGATTTGGAGAACTTCTGATGTCATCCGGGATTGTGTTGAATGATAATGTTCATTGGGTTACTTTTCACAGTGGTTATGATTTTGGTTATCTTTTGAAGCTTTTGACGTGCAGGGCTTTGCCGGATACACAAGCTGGATTTTTCGATTTGATCGGGATTTATTTCCCGATTGTGTATGATATCAAACATTTGATGAAGTTCTGTAACAGTCTTCATGGTGGTTTGAACAAGCTTGCGGAATTGCTGGAAGTTGAGAGGGTTGGTGTGTGCCATCAAGCTGGATCGGATAGTTTACTCACAGCTTGTACATTTAGGAAGTTGAGGGAAACATTCTTCAACGGCGAAACAGAGAAATATTCTGGTGTGTTGTATGGATTAGGTGTTGAGAAAACAGACTAA

Protein sequence

>MS.gene42653.t1

MTDLTKDLQKGDSIQIREVWNDNLEEEFVLIREIVDKYNYVAMDTEFPGVVLRPVGNFKHINDFNYQTLKDNVDMLKLIQLGLTFSDENGNLPTCGTDSPCIWQFNFREFNVSEDIFAADSIELLRQCGIDFKKNSEQGIDVNRFGELLMSSGIVLNDNVHWVTFHSGYDFGYLLKLLTCRALPDTQAGFFDLIGIYFPIVYDIKHLMKFCNSLHGGLNKLAELLEVERVGVCHQAGSDSLLTACTFRKLRETFFNGETEKYSGVLYGLGVEKTD