AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32688


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32688.t1 AT2G32070 78.030 264 57 1 10 272 6 269 8.23e-155 432
MS.gene32688.t1 AT1G80780 77.567 263 59 0 10 272 6 268 6.29e-154 430
MS.gene32688.t1 AT1G80780 77.567 263 59 0 10 272 6 268 6.29e-154 430
MS.gene32688.t1 AT1G80780 77.567 263 59 0 10 272 6 268 6.67e-154 431
MS.gene32688.t1 AT5G10960 76.806 263 61 0 10 272 6 268 6.14e-153 428
MS.gene32688.t1 AT1G15920 61.091 275 101 1 1 269 1 275 9.35e-120 344
MS.gene32688.t1 AT1G15920 61.091 275 101 1 1 269 1 275 9.35e-120 344
MS.gene32688.t1 AT3G44260 53.846 273 118 7 6 271 8 279 2.09e-92 275
MS.gene32688.t1 AT5G22250 52.985 268 118 6 12 271 10 277 4.12e-89 266
MS.gene32688.t1 AT1G06450 36.680 259 146 7 17 270 10 255 2.38e-45 156
MS.gene32688.t1 AT1G61470 35.743 249 140 6 18 260 4 238 3.56e-37 133
MS.gene32688.t1 AT3G44240 37.719 228 128 6 30 254 3 219 9.04e-36 128
MS.gene32688.t1 AT1G27820 32.806 253 154 6 18 265 8 249 4.39e-31 117
MS.gene32688.t1 AT1G27890 33.468 248 149 6 18 260 3 239 1.80e-28 110

Find 0 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATACTTATCAACAATTTCA+CGG + 24279:17856-17875 None:intergenic 20.0%
! TGTTTGATATCATACACAAT+CGG + 24279:18358-18377 None:intergenic 25.0%
!! GTTGAATGATAATGTTCATT+GGG - 24279:18225-18244 MS.gene32688:CDS 25.0%
!! TGTTGAATGATAATGTTCAT+TGG - 24279:18224-18243 MS.gene32688:CDS 25.0%
!!! TTAACTTTTTCTGATGAGAA+TGG - 24279:18007-18026 MS.gene32688:CDS 25.0%
!!! TTTTAACTATCAGACTTTGA+AGG - 24279:17949-17968 MS.gene32688:CDS 25.0%
AAATATTCTGGTGTGTTGTA+TGG - 24279:18541-18560 MS.gene32688:CDS 30.0%
ATATGCTTAAAATTCCCAAC+AGG + 24279:17923-17942 None:intergenic 30.0%
ATTATCATTCAACACAATCC+CGG + 24279:18218-18237 None:intergenic 30.0%
GTTTGATATCATACACAATC+GGG + 24279:18357-18376 None:intergenic 30.0%
TAAGTATAACTATGTTGCGA+TGG - 24279:17868-17887 MS.gene32688:CDS 30.0%
TCTTAAAATCAATTCCGCAT+TGG + 24279:18141-18160 None:intergenic 30.0%
TTAACGAAGGATTTACAGAA+AGG - 24279:17770-17789 MS.gene32688:CDS 30.0%
TTGTACATTTAGGAAGTTGA+GGG - 24279:18492-18511 MS.gene32688:CDS 30.0%
! TTTCACAGTGGTTATGATTT+TGG - 24279:18253-18272 MS.gene32688:CDS 30.0%
AAAAATCCAGCTTGTGTATC+CGG + 24279:18313-18332 None:intergenic 35.0%
AAATTGAATTGCCAGATGCA+AGG + 24279:18061-18080 None:intergenic 35.0%
AAGTTCTGTAACAGTCTTCA+TGG - 24279:18385-18404 MS.gene32688:CDS 35.0%
AATGGAAATTTACCTACCTG+TGG - 24279:18025-18044 MS.gene32688:CDS 35.0%
AGATTCGATTCAAATCAGAG+AGG - 24279:17793-17812 MS.gene32688:CDS 35.0%
CTTGTACATTTAGGAAGTTG+AGG - 24279:18491-18510 MS.gene32688:CDS 35.0%
TACTCACAGCTTGTACATTT+AGG - 24279:18482-18501 MS.gene32688:CDS 35.0%
TTGAGGGAAACATTCTTCAA+CGG - 24279:18508-18527 MS.gene32688:CDS 35.0%
! ATGTTGAAGCTGATTCAGTT+AGG - 24279:17983-18002 MS.gene32688:CDS 35.0%
!! CTGGATTTTTCGATTTGATC+GGG - 24279:18323-18342 MS.gene32688:CDS 35.0%
!! GCTGGATTTTTCGATTTGAT+CGG - 24279:18322-18341 MS.gene32688:CDS 35.0%
ATGGAACGATAATCTGGAAG+AGG - 24279:17817-17836 MS.gene32688:CDS 40.0%
CGATTCAAATCAGAGAGGTA+TGG - 24279:17798-17817 MS.gene32688:CDS 40.0%
GGCGAAACAGAGAAATATTC+TGG - 24279:18529-18548 MS.gene32688:CDS 40.0%
TCTGGTGTGTTGTATGGATT+AGG - 24279:18547-18566 MS.gene32688:CDS 40.0%
TTCTGTAACAGTCTTCATGG+TGG - 24279:18388-18407 MS.gene32688:CDS 40.0%
TTTAAGAAGAACAGCGAGCA+AGG - 24279:18154-18173 MS.gene32688:CDS 40.0%
! AGAGGTATGGAACGATAATC+TGG - 24279:17811-17830 MS.gene32688:CDS 40.0%
!! AGTTTCCTGGTGTTGTTCTT+CGG - 24279:17897-17916 MS.gene32688:CDS 40.0%
!! TTTGAAGCTTTTGACGTGCA+GGG - 24279:18282-18301 MS.gene32688:CDS 40.0%
!!! CATTGGGTTACTTTTCACAG+TGG - 24279:18241-18260 MS.gene32688:CDS 40.0%
!!! TTTTGAAGCTTTTGACGTGC+AGG - 24279:18281-18300 MS.gene32688:CDS 40.0%
AACTATCCGATCCAGCTTGA+TGG + 24279:18462-18481 None:intergenic 45.0%
GAGAACTTCTGATGTCATCC+GGG - 24279:18197-18216 MS.gene32688:CDS 45.0%
GGAATCGACGTGAACAGATT+TGG - 24279:18175-18194 MS.gene32688:CDS 45.0%
GGAATTGCTGGAAGTTGAGA+GGG - 24279:18426-18445 MS.gene32688:CDS 45.0%
GGAGAACTTCTGATGTCATC+CGG - 24279:18196-18215 MS.gene32688:CDS 45.0%
TGGTGGTTTGAACAAGCTTG+CGG - 24279:18405-18424 MS.gene32688:CDS 45.0%
CGGAATTGCTGGAAGTTGAG+AGG - 24279:18425-18444 MS.gene32688:CDS 50.0%
GAACAAGCTTGCGGAATTGC+TGG - 24279:18414-18433 MS.gene32688:CDS 50.0%
GCGATGGATACAGAGTTTCC+TGG - 24279:17884-17903 MS.gene32688:CDS 50.0%
TGTGTGCCATCAAGCTGGAT+CGG - 24279:18453-18472 MS.gene32688:CDS 50.0%
TTGCTGGAAGTTGAGAGGGT+TGG - 24279:18430-18449 MS.gene32688:CDS 50.0%
! ATCGAGCTTTTGCGCCAATG+CGG - 24279:18124-18143 MS.gene32688:CDS 50.0%
! GCACTGATAGTCCTTGCATC+TGG - 24279:18047-18066 MS.gene32688:CDS 50.0%
! GTGTTGTTCTTCGGCCTGTT+GGG - 24279:17906-17925 MS.gene32688:CDS 50.0%
ACAGGCCGAAGAACAACACC+AGG + 24279:17905-17924 None:intergenic 55.0%
GCAAGGACTATCAGTGCCAC+AGG + 24279:18044-18063 None:intergenic 55.0%
GCTTTGCCGGATACACAAGC+TGG - 24279:18304-18323 MS.gene32688:CDS 55.0%
GGACTATCAGTGCCACAGGT+AGG + 24279:18040-18059 None:intergenic 55.0%
GTTGGTGTGTGCCATCAAGC+TGG - 24279:18448-18467 MS.gene32688:CDS 55.0%
TTTGACGTGCAGGGCTTTGC+CGG - 24279:18291-18310 MS.gene32688:CDS 55.0%
!! GGTGTTGTTCTTCGGCCTGT+TGG - 24279:17905-17924 MS.gene32688:CDS 55.0%


Chromosome Type Strat End Strand Name
24279 gene 17761 18588 17761 ID=MS.gene32688
24279 mRNA 17761 18588 17761 ID=MS.gene32688.t1;Parent=MS.gene32688
24279 exon 17761 18588 17761 ID=MS.gene32688.t1.exon1;Parent=MS.gene32688.t1
24279 CDS 17761 18588 17761 ID=cds.MS.gene32688.t1;Parent=MS.gene32688.t1
Gene Sequence

>MS.gene32688

ATGACGGATTTAACGAAGGATTTACAGAAAGGAGATTCGATTCAAATCAGAGAGGTATGGAACGATAATCTGGAAGAGGAATTTGTGTTGATCCGTGAAATTGTTGATAAGTATAACTATGTTGCGATGGATACAGAGTTTCCTGGTGTTGTTCTTCGGCCTGTTGGGAATTTTAAGCATATTAACGATTTTAACTATCAGACTTTGAAGGATAACGTTGATATGTTGAAGCTGATTCAGTTAGGTTTAACTTTTTCTGATGAGAATGGAAATTTACCTACCTGTGGCACTGATAGTCCTTGCATCTGGCAATTCAATTTTCGCGAGTTTAATGTTAGCGAAGATATTTTCGCTGCTGATTCGATCGAGCTTTTGCGCCAATGCGGAATTGATTTTAAGAAGAACAGCGAGCAAGGAATCGACGTGAACAGATTTGGAGAACTTCTGATGTCATCCGGGATTGTGTTGAATGATAATGTTCATTGGGTTACTTTTCACAGTGGTTATGATTTTGGTTATCTTTTGAAGCTTTTGACGTGCAGGGCTTTGCCGGATACACAAGCTGGATTTTTCGATTTGATCGGGATTTATTTCCCGATTGTGTATGATATCAAACATTTGATGAAGTTCTGTAACAGTCTTCATGGTGGTTTGAACAAGCTTGCGGAATTGCTGGAAGTTGAGAGGGTTGGTGTGTGCCATCAAGCTGGATCGGATAGTTTACTCACAGCTTGTACATTTAGGAAGTTGAGGGAAACATTCTTCAACGGCGAAACAGAGAAATATTCTGGTGTGTTGTATGGATTAGGTGTTGAGAAAACAGACTAA

Protein sequence

>MS.gene32688.t1

MTDLTKDLQKGDSIQIREVWNDNLEEEFVLIREIVDKYNYVAMDTEFPGVVLRPVGNFKHINDFNYQTLKDNVDMLKLIQLGLTFSDENGNLPTCGTDSPCIWQFNFREFNVSEDIFAADSIELLRQCGIDFKKNSEQGIDVNRFGELLMSSGIVLNDNVHWVTFHSGYDFGYLLKLLTCRALPDTQAGFFDLIGIYFPIVYDIKHLMKFCNSLHGGLNKLAELLEVERVGVCHQAGSDSLLTACTFRKLRETFFNGETEKYSGVLYGLGVEKTD