AlfalfaGEDB | MS.gene90263

M. sativa cultivar XinJiangDaYe / MS.gene90263

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00439.28 PF17035.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90263.t1	KEH31196.1	90.7	484	45	0	1	484	1	484	6.90E-222	780

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90263.t1	Q7Y214	37.7	557	246	11	1	484	1	529	2.2e-75	284.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90263.t1	A0A072UP94	90.7	484	45	0	1	484	1	484	5.0e-222	780.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene90263	0.850253	1.91E-60	-1.69E-46
MS.gene049807	MS.gene90263	0.803433	3.49E-49	-1.69E-46
MS.gene049983	MS.gene90263	0.827859	1.24E-54	-1.69E-46
MS.gene04998	MS.gene90263	0.847014	1.51E-59	-1.69E-46
MS.gene050359	MS.gene90263	0.830094	3.56E-55	-1.69E-46
MS.gene05035	MS.gene90263	0.815751	7.88E-52	-1.69E-46
MS.gene050414	MS.gene90263	0.888052	8.30E-73	-1.69E-46
MS.gene050646	MS.gene90263	0.84434	8.03E-59	-1.69E-46
MS.gene050654	MS.gene90263	0.849607	2.90E-60	-1.69E-46
MS.gene050945	MS.gene90263	0.816152	6.41E-52	-1.69E-46
MS.gene051043	MS.gene90263	0.819147	1.35E-52	-1.69E-46
MS.gene051184	MS.gene90263	0.80028	1.55E-48	-1.69E-46
MS.gene051226	MS.gene90263	0.805328	1.41E-49	-1.69E-46
MS.gene051279	MS.gene90263	0.816167	6.36E-52	-1.69E-46
MS.gene051628	MS.gene90263	0.802549	5.32E-49	-1.69E-46
MS.gene051819	MS.gene90263	0.818364	2.04E-52	-1.69E-46
MS.gene051852	MS.gene90263	0.845474	3.97E-59	-1.69E-46
MS.gene051856	MS.gene90263	0.800992	1.11E-48	-1.69E-46
MS.gene052016	MS.gene90263	0.86514	7.33E-65	-1.69E-46
MS.gene052052	MS.gene90263	0.800461	1.43E-48	-1.69E-46
MS.gene052239	MS.gene90263	-0.815692	8.11E-52	-1.69E-46
MS.gene052242	MS.gene90263	-0.81873	1.68E-52	-1.69E-46
MS.gene052307	MS.gene90263	0.85531	6.87E-62	-1.69E-46
MS.gene052463	MS.gene90263	0.838306	3.11E-57	-1.69E-46
MS.gene052493	MS.gene90263	0.809857	1.54E-50	-1.69E-46
MS.gene052533	MS.gene90263	0.845033	5.23E-59	-1.69E-46
MS.gene052609	MS.gene90263	0.84718	1.36E-59	-1.69E-46
MS.gene052775	MS.gene90263	0.80006	1.72E-48	-1.69E-46
MS.gene052776	MS.gene90263	0.820881	5.43E-53	-1.69E-46
MS.gene052932	MS.gene90263	0.81416	1.77E-51	-1.69E-46
MS.gene053079	MS.gene90263	0.831563	1.56E-55	-1.69E-46
MS.gene053143	MS.gene90263	0.823961	1.04E-53	-1.69E-46
MS.gene053486	MS.gene90263	0.806365	8.52E-50	-1.69E-46
MS.gene053575	MS.gene90263	0.800971	1.12E-48	-1.69E-46
MS.gene053618	MS.gene90263	0.82835	9.44E-55	-1.69E-46
MS.gene053620	MS.gene90263	0.873581	1.32E-67	-1.69E-46
MS.gene053726	MS.gene90263	0.82193	3.11E-53	-1.69E-46
MS.gene054178	MS.gene90263	0.844534	7.12E-59	-1.69E-46
MS.gene054222	MS.gene90263	0.815187	1.05E-51	-1.69E-46
MS.gene054576	MS.gene90263	0.811117	8.22E-51	-1.69E-46
MS.gene054641	MS.gene90263	0.812109	5.00E-51	-1.69E-46
MS.gene054824	MS.gene90263	0.825861	3.72E-54	-1.69E-46
MS.gene054892	MS.gene90263	0.871504	6.50E-67	-1.69E-46
MS.gene054893	MS.gene90263	0.875403	3.17E-68	-1.69E-46
MS.gene055231	MS.gene90263	0.805871	1.08E-49	-1.69E-46
MS.gene055386	MS.gene90263	0.804633	1.97E-49	-1.69E-46
MS.gene05539	MS.gene90263	0.813861	2.07E-51	-1.69E-46
MS.gene055513	MS.gene90263	0.830641	2.62E-55	-1.69E-46
MS.gene055864	MS.gene90263	0.835485	1.63E-56	-1.69E-46
MS.gene056045	MS.gene90263	0.826118	3.23E-54	-1.69E-46
MS.gene056097	MS.gene90263	0.806502	7.97E-50	-1.69E-46
MS.gene056181	MS.gene90263	0.81215	4.90E-51	-1.69E-46
MS.gene056507	MS.gene90263	0.82608	3.30E-54	-1.69E-46
MS.gene056699	MS.gene90263	0.805015	1.64E-49	-1.69E-46
MS.gene05686	MS.gene90263	0.802035	6.79E-49	-1.69E-46
MS.gene056973	MS.gene90263	0.80122	9.98E-49	-1.69E-46
MS.gene057034	MS.gene90263	0.805756	1.14E-49	-1.69E-46
MS.gene057102	MS.gene90263	0.814338	1.62E-51	-1.69E-46
MS.gene057919	MS.gene90263	0.83557	1.55E-56	-1.69E-46
MS.gene058159	MS.gene90263	0.802056	6.72E-49	-1.69E-46
MS.gene058289	MS.gene90263	0.844467	7.42E-59	-1.69E-46
MS.gene058330	MS.gene90263	0.817808	2.72E-52	-1.69E-46
MS.gene058638	MS.gene90263	0.818783	1.64E-52	-1.69E-46
MS.gene058639	MS.gene90263	0.821803	3.32E-53	-1.69E-46
MS.gene058649	MS.gene90263	0.80812	3.62E-50	-1.69E-46
MS.gene058758	MS.gene90263	0.800795	1.22E-48	-1.69E-46
MS.gene059007	MS.gene90263	0.820055	8.39E-53	-1.69E-46
MS.gene059170	MS.gene90263	0.816499	5.36E-52	-1.69E-46
MS.gene059965	MS.gene90263	0.849605	2.90E-60	-1.69E-46
MS.gene060259	MS.gene90263	0.820374	7.10E-53	-1.69E-46
MS.gene060373	MS.gene90263	0.806888	6.61E-50	-1.69E-46
MS.gene060413	MS.gene90263	0.800345	1.51E-48	-1.69E-46
MS.gene060435	MS.gene90263	0.823438	1.39E-53	-1.69E-46
MS.gene06052	MS.gene90263	0.815327	9.78E-52	-1.69E-46
MS.gene060840	MS.gene90263	0.808821	2.57E-50	-1.69E-46
MS.gene060932	MS.gene90263	0.838634	2.56E-57	-1.69E-46
MS.gene060933	MS.gene90263	0.846011	2.84E-59	-1.69E-46
MS.gene060937	MS.gene90263	0.81021	1.29E-50	-1.69E-46
MS.gene061006	MS.gene90263	0.806894	6.59E-50	-1.69E-46
MS.gene061100	MS.gene90263	0.8407	7.42E-58	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene90263	MS.gene029093	PPI
MS.gene064542	MS.gene90263	PPI
MS.gene90263	MS.gene019660	PPI
MS.gene056804	MS.gene90263	PPI
MS.gene029217	MS.gene90263	PPI
MS.gene035805	MS.gene90263	PPI
MS.gene23912	MS.gene90263	PPI
MS.gene037356	MS.gene90263	PPI
MS.gene84252	MS.gene90263	PPI
MS.gene93135	MS.gene90263	PPI
MS.gene066439	MS.gene90263	PPI
MS.gene029219	MS.gene90263	PPI
MS.gene90263	MS.gene90825	PPI
MS.gene90263	MS.gene73119	PPI
MS.gene90263	MS.gene97346	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90263.t1	MTR_4g092900	95.872	533	22	0	1	533	1	533	0.0	976
MS.gene90263.t1	MTR_4g092900	95.740	493	21	0	1	493	1	493	0.0	969
MS.gene90263.t1	MTR_4g092880	82.657	542	53	7	1	533	1	510	0.0	822
MS.gene90263.t1	MTR_4g092880	83.155	374	47	5	1	366	1	366	0.0	615
MS.gene90263.t1	MTR_2g091130	45.763	295	132	6	131	425	283	549	7.29e-69	233
MS.gene90263.t1	MTR_2g091135	44.027	293	120	7	141	425	304	560	8.40e-64	219
MS.gene90263.t1	MTR_2g091135	44.027	293	120	7	141	425	304	560	1.08e-63	219
MS.gene90263.t1	MTR_4g079650	40.278	288	101	9	145	425	656	879	5.75e-50	185
MS.gene90263.t1	MTR_4g079650	40.278	288	101	9	145	425	656	879	5.76e-50	185
MS.gene90263.t1	MTR_4g079650	42.041	245	98	8	145	382	656	863	6.91e-46	172
MS.gene90263.t1	MTR_3g075040	27.651	481	233	14	51	487	79	488	1.19e-40	155
MS.gene90263.t1	MTR_3g075040	27.651	481	233	14	51	487	79	488	3.03e-40	155
MS.gene90263.t1	MTR_6g033375	26.933	375	212	10	97	460	13	336	9.22e-27	111
MS.gene90263.t1	MTR_2g018270	40.571	175	91	5	95	266	29	193	1.31e-26	113
MS.gene90263.t1	MTR_8g070660	25.786	318	188	8	98	411	46	319	2.95e-24	104
MS.gene90263.t1	MTR_8g070660	36.184	152	88	5	98	245	46	192	8.74e-21	92.8
MS.gene90263.t1	MTR_5g069490	25.969	258	132	7	159	413	111	312	1.35e-16	81.6
MS.gene90263.t1	MTR_2g437920	44.444	81	42	2	167	244	149	229	4.30e-14	74.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90263.t1	AT1G73150	38.287	397	208	9	37	429	23	386	8.62e-63	212
MS.gene90263.t1	AT1G73150	38.287	397	208	9	37	429	23	386	1.08e-62	213
MS.gene90263.t1	AT1G17790	37.995	379	194	10	55	420	49	399	4.63e-59	203
MS.gene90263.t1	AT1G06230	42.647	272	133	6	144	414	421	670	1.17e-53	194
MS.gene90263.t1	AT1G06230	42.647	272	133	6	144	414	421	670	1.17e-53	194
MS.gene90263.t1	AT1G06230	42.647	272	133	6	144	414	421	670	1.17e-53	194
MS.gene90263.t1	AT1G06230	42.647	272	133	6	144	414	421	670	1.17e-53	194
MS.gene90263.t1	AT5G65630	60.265	151	52	1	337	487	390	532	2.14e-48	177
MS.gene90263.t1	AT5G10550	56.000	150	59	2	337	486	385	527	1.21e-42	160
MS.gene90263.t1	AT5G14270	30.317	442	219	14	77	503	64	431	1.01e-39	154
MS.gene90263.t1	AT5G14270	30.317	442	219	14	77	503	64	431	1.01e-39	154
MS.gene90263.t1	AT5G14270	30.317	442	219	14	77	503	64	431	1.27e-39	153
MS.gene90263.t1	AT5G63320	28.821	458	216	17	64	487	60	441	1.22e-38	147
MS.gene90263.t1	AT5G63320	28.821	458	216	17	64	487	60	441	1.22e-38	147
MS.gene90263.t1	AT5G63320	28.821	458	216	17	64	487	60	441	6.52e-37	146
MS.gene90263.t1	AT3G27260	31.522	368	176	13	104	469	142	435	1.58e-33	135
MS.gene90263.t1	AT3G27260	31.522	368	176	13	104	469	142	435	2.07e-33	135
MS.gene90263.t1	AT3G27260	31.522	368	176	13	104	469	142	435	2.24e-33	135
MS.gene90263.t1	AT3G27260	31.522	368	176	13	104	469	93	386	2.28e-33	135
MS.gene90263.t1	AT3G01770	30.588	425	206	15	82	492	60	409	8.47e-33	131
MS.gene90263.t1	AT3G01770	32.386	352	162	12	145	492	130	409	5.93e-32	130
MS.gene90263.t1	AT3G52280	27.206	272	151	6	143	411	93	320	2.89e-24	104
MS.gene90263.t1	AT5G46550	40.741	135	67	2	148	282	73	194	4.28e-24	105
MS.gene90263.t1	AT3G52280	27.206	272	151	6	143	411	110	337	4.44e-24	104
MS.gene90263.t1	AT2G34900	27.062	388	222	11	87	460	45	385	1.92e-23	102
MS.gene90263.t1	AT2G34900	27.907	301	169	5	166	460	17	275	5.44e-23	99.4
MS.gene90263.t1	AT1G58025	36.036	111	61	3	134	243	209	310	4.99e-11	65.9
MS.gene90263.t1	AT1G58025	36.036	111	61	3	134	243	209	310	4.99e-11	65.9
MS.gene90263.t1	AT1G58025	36.036	111	61	3	134	243	209	310	4.99e-11	65.9
MS.gene90263.t1	AT1G58025	36.036	111	61	3	134	243	209	310	5.08e-11	65.9
MS.gene90263.t1	AT1G58025	36.036	111	61	3	134	243	209	310	5.08e-11	65.9
MS.gene90263.t1	AT1G58025	36.036	111	61	3	134	243	209	310	5.42e-11	65.5

Find 159 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACATTCATTTCCCTCTTATT+TGG	0.124253	4.2:+20247301	None:intergenic
CTTTGGCTTGGGCTGCTTCA+AGG	0.184318	4.2:+20247333	None:intergenic
CTTCATGCAGATTCGGATTC+AGG	0.197155	4.2:-20246615	MS.gene90263:intron
TTCAAAGCCGTTGCATTTAC+AGG	0.229221	4.2:+20247823	None:intergenic
CCTCTGTGTTGCAGCCAATT+TGG	0.258953	4.2:+20248303	None:intergenic
TCCAGTGGTTCCAGCAGCTC+TGG	0.278947	4.2:-20246730	MS.gene90263:CDS
ACCCTCTCCCTTTCCCTCTC+TGG	0.292850	4.2:+20247520	None:intergenic
TTGTTACTAAATTTAGGGTT+AGG	0.292960	4.2:+20248246	None:intergenic
TCAGGGAGCAAGAAGGTTTC+CGG	0.305508	4.2:-20247991	MS.gene90263:CDS
GAGTCTTAAGCTGACGAATT+TGG	0.320746	4.2:+20248076	None:intergenic
GGATCCCTGGCCTTTGGCTT+GGG	0.322222	4.2:+20247322	None:intergenic
TTCCCTCTTATTTGGATCCC+TGG	0.326630	4.2:+20247309	None:intergenic
TGGATCCCTGGCCTTTGGCT+TGG	0.328127	4.2:+20247321	None:intergenic
TTTGGCTTGGGCTGCTTCAA+GGG	0.331789	4.2:+20247334	None:intergenic
GCATCAACCTTCTCCCTATC+AGG	0.335506	4.2:+20246907	None:intergenic
TCGGATTCAGGGAGTTCTTC+AGG	0.341320	4.2:-20246603	MS.gene90263:CDS
TCACTGGAAGTGCTCGCGTT+GGG	0.343551	4.2:+20247359	None:intergenic
TTATTTGGATCCCTGGCCTT+TGG	0.344705	4.2:+20247316	None:intergenic
ACCCCATTGCCTTTGTTTGA+AGG	0.345351	4.2:+20247040	None:intergenic
GGCTGCTTCAAGGGCTTCAC+TGG	0.348486	4.2:+20247343	None:intergenic
ACCACCGTTGTGGTTTAGCC+TGG	0.356873	4.2:+20248029	None:intergenic
ACCGGTGGAGGGATTGGAAT+TGG	0.357465	4.2:+20247481	None:intergenic
CTGTTGACACGGAGACTCTC+TGG	0.367522	4.2:-20247144	MS.gene90263:CDS
CAGGCTCAGGCAGTGGCTCT+TGG	0.369449	4.2:+20247449	None:intergenic
AATTAAGCATCCTATGGATC+TGG	0.371201	4.2:-20247779	MS.gene90263:CDS
CGAACTGGTGACTGAGCTAA+TGG	0.371584	4.2:+20247391	None:intergenic
ACAACCGTAACGAACCAAAT+TGG	0.373779	4.2:-20248317	MS.gene90263:CDS
GATTAAGCGGCAGGCGCTAA+TGG	0.380098	4.2:-20247077	MS.gene90263:CDS
AAGAAATCAAAGTCAGAGAT+TGG	0.382024	4.2:-20247934	MS.gene90263:CDS
TCATCAAATTTCTCGTGTAT+TGG	0.386676	4.2:+20247592	None:intergenic
TTCTGTTTCTTTCCCTCAGA+AGG	0.387137	4.2:+20246877	None:intergenic
CTTGCTCCCTGATTTCTTAG+TGG	0.395685	4.2:+20248002	None:intergenic
TTGCTCCCTGATTTCTTAGT+GGG	0.400065	4.2:+20248003	None:intergenic
GATTGAGCAAGATAAAGATA+TGG	0.403309	4.2:-20246782	MS.gene90263:CDS
GGTGGTCCCACTAAGAAATC+AGG	0.406385	4.2:-20248009	MS.gene90263:CDS
TTTGCTACCGGTGGAGGGAT+TGG	0.415792	4.2:+20247475	None:intergenic
CTTAAGACTCGAATTGAATC+AGG	0.417831	4.2:-20248063	MS.gene90263:CDS
TGCCTTCAAACAAAGGCAAT+GGG	0.422340	4.2:-20247042	MS.gene90263:CDS
AGGAAAAGGTCGTTTGTTGC+CGG	0.425299	4.2:+20247972	None:intergenic
ACTACTTGTTCCATCTTCTC+TGG	0.427581	4.2:+20247235	None:intergenic
AAGGAAGAGAAATGGGCATT+TGG	0.428825	4.2:-20247203	MS.gene90263:CDS
CTAAATTTAGGGTTAGGGTT+AGG	0.433996	4.2:+20248252	None:intergenic
AGAAATCAAAGTCAGAGATT+GGG	0.434900	4.2:-20247933	MS.gene90263:CDS
GGATGAGATTGAGCTTGATA+TGG	0.436408	4.2:-20247170	MS.gene90263:CDS
ATTTACAGGAATTGCCTGAT+AGG	0.443388	4.2:-20246921	MS.gene90263:intron
CCCAGAGCTGCTGGAACCAC+TGG	0.444706	4.2:+20246729	None:intergenic
TCGATCGATTGGTTACCAAT+TGG	0.445939	4.2:-20247117	MS.gene90263:CDS
TGTTACTAAATTTAGGGTTA+GGG	0.446081	4.2:+20248247	None:intergenic
AGGCGGTGGAGCTGAATTCA+TGG	0.450284	4.2:-20248283	MS.gene90263:CDS
TGATATAATTAAGCATCCTA+TGG	0.454884	4.2:-20247785	MS.gene90263:CDS
ACAGGTGGGAAATCGTTTGC+TGG	0.455112	4.2:+20246808	None:intergenic
AATTCCTTCACCGCAGGAAA+AGG	0.459833	4.2:+20247958	None:intergenic
AGATCATAAGGAAGAGAAAT+GGG	0.462775	4.2:-20247210	MS.gene90263:CDS
AACAAGTAGTACAGATCATA+AGG	0.463491	4.2:-20247222	MS.gene90263:CDS
ATGGGCATTTGGAGCAAGAT+GGG	0.463782	4.2:-20247192	MS.gene90263:CDS
GCAGCCAATTTGGTTCGTTA+CGG	0.464960	4.2:+20248313	None:intergenic
TCAGGAAGTGATTCTGAAGC+TGG	0.466135	4.2:-20246585	MS.gene90263:CDS
ATTGAGCAAGATAAAGATAT+GGG	0.469983	4.2:-20246781	MS.gene90263:CDS
GTTTCTTTCCCTCAGAAGGA+GGG	0.472060	4.2:+20246881	None:intergenic
GGGGATTCAAAGTGCTTGGC+TGG	0.473093	4.2:+20247413	None:intergenic
CAGATCATAAGGAAGAGAAA+TGG	0.474728	4.2:-20247211	MS.gene90263:CDS
GTTGGGGAGGGTATGCGAAC+TGG	0.475235	4.2:+20247376	None:intergenic
TGGAAGTGCTCGCGTTGGGG+AGG	0.475254	4.2:+20247363	None:intergenic
TTCATGCAGATTCGGATTCA+GGG	0.475639	4.2:-20246614	MS.gene90263:intron
GATAAAGATATGGGTGGTAC+TGG	0.476434	4.2:-20246772	MS.gene90263:CDS
GGAATCACTCCCAGAGCTGC+TGG	0.477857	4.2:+20246720	None:intergenic
TTCACTGGAAGTGCTCGCGT+TGG	0.478335	4.2:+20247358	None:intergenic
AGCAGCCCAAGCCAAAGGCC+AGG	0.478818	4.2:-20247327	MS.gene90263:CDS
GTGTTAACATCATGGCCCTT+GGG	0.480899	4.2:+20247658	None:intergenic
AGGCCAGGGATCCAAATAAG+AGG	0.482051	4.2:-20247312	MS.gene90263:CDS
TTGATGAGGATGTTGACATC+GGG	0.489567	4.2:-20246840	MS.gene90263:CDS
AGAGATTGGGGATGCGATGA+AGG	0.491863	4.2:-20247920	MS.gene90263:CDS
TGTTTCTTTCCCTCAGAAGG+AGG	0.493964	4.2:+20246880	None:intergenic
GTACTAGCAGGCTCAGGCAG+TGG	0.495175	4.2:+20247442	None:intergenic
GGAACCACTGGAACTACTAC+TGG	0.498470	4.2:+20246741	None:intergenic
TTTCTTTCCCTCAGAAGGAG+GGG	0.500315	4.2:+20246882	None:intergenic
GCAGCCCAAGCCAAAGGCCA+GGG	0.500330	4.2:-20247326	MS.gene90263:CDS
CCAGTGGTTCCAGCAGCTCT+GGG	0.500740	4.2:-20246729	MS.gene90263:CDS
AGTGTTAACATCATGGCCCT+TGG	0.504161	4.2:+20247657	None:intergenic
GTTGGATCGTCGATTGATTG+AGG	0.506931	4.2:+20248191	None:intergenic
TTCTTCAATTCCTTCACCGC+AGG	0.508468	4.2:+20247952	None:intergenic
TTTCCCTCTCTGGTTCAACA+TGG	0.510397	4.2:+20247530	None:intergenic
ATGTTGAACCAGAGAGGGAA+AGG	0.513945	4.2:-20247528	MS.gene90263:CDS
TTATCTTGCTCAATCTCCAC+AGG	0.515091	4.2:+20246790	None:intergenic
AACCAGAGAGGGAAAGGGAG+AGG	0.515203	4.2:-20247522	MS.gene90263:CDS
GGTGAGCAAGATTAAGCGGC+AGG	0.516928	4.2:-20247086	MS.gene90263:CDS
TTTACAGGAATTGCCTGATA+GGG	0.522059	4.2:-20246920	MS.gene90263:intron
AATGGGCATTTGGAGCAAGA+TGG	0.522107	4.2:-20247193	MS.gene90263:CDS
ATTGAGGAAGCGTCATCTGA+TGG	0.524535	4.2:+20248207	None:intergenic
TGGCTGCAACACAGAGGCGG+TGG	0.525481	4.2:-20248297	MS.gene90263:CDS
GTGCCTTCAAACAAAGGCAA+TGG	0.528594	4.2:-20247043	MS.gene90263:CDS
CACTGGAAGTGCTCGCGTTG+GGG	0.529411	4.2:+20247360	None:intergenic
CCACCGTTGTGGTTTAGCCT+GGG	0.530502	4.2:+20248030	None:intergenic
TTGAGGAAGCGTCATCTGAT+GGG	0.538515	4.2:+20248208	None:intergenic
ATTAAGCATCCTATGGATCT+GGG	0.539150	4.2:-20247778	MS.gene90263:CDS
GAACTGGTGACTGAGCTAAT+GGG	0.540318	4.2:+20247392	None:intergenic
GGAAGTGCTCGCGTTGGGGA+GGG	0.542081	4.2:+20247364	None:intergenic
TTAGAAGTACTAGCAGGCTC+AGG	0.542104	4.2:+20247436	None:intergenic
GTATTGTGAATGATTATCGT+TGG	0.542418	4.2:+20248173	None:intergenic
GAAATCAAAGTCAGAGATTG+GGG	0.550032	4.2:-20247932	MS.gene90263:CDS
TGATGCAACCCCTCCTTCTG+AGG	0.557199	4.2:-20246890	MS.gene90263:CDS
AATAATGTGCCTTCAAACAA+AGG	0.557916	4.2:-20247049	MS.gene90263:CDS
ACTTGTTCCATCTTCTCTGG+TGG	0.558187	4.2:+20247238	None:intergenic
GGCTGGTTAGAAGTACTAGC+AGG	0.564980	4.2:+20247430	None:intergenic
TGTTGACACGGAGACTCTCT+GGG	0.565659	4.2:-20247143	MS.gene90263:CDS
TGTTGAACCAGAGAGGGAAA+GGG	0.566133	4.2:-20247527	MS.gene90263:CDS
GGCGGTGGAGCTGAATTCAT+GGG	0.566531	4.2:-20248282	MS.gene90263:CDS
CTTGAAGCAGCCCAAGCCAA+AGG	0.568683	4.2:-20247332	MS.gene90263:CDS
ACCAGAGAGGGAAAGGGAGA+GGG	0.569026	4.2:-20247521	MS.gene90263:CDS
TCCAATTCCAATCCCTCCAC+CGG	0.570278	4.2:-20247482	MS.gene90263:CDS
TCTTGCTCAATCTCCACAGG+TGG	0.571533	4.2:+20246793	None:intergenic
TCTCTGGGAACTCGATCGAT+TGG	0.573945	4.2:-20247128	MS.gene90263:CDS
TCGCCGCAGTGTTAACATCA+TGG	0.579958	4.2:+20247650	None:intergenic
ATGAATTACAGGCTAGCTCG+TGG	0.582142	4.2:-20247555	MS.gene90263:CDS
AGCAAACGATTTCCCACCTG+TGG	0.582684	4.2:-20246806	MS.gene90263:CDS
CATCGAATCGTACACAAAGA+CGG	0.584140	4.2:-20248139	MS.gene90263:CDS
TAGTGGGACCACCACCGTTG+TGG	0.584586	4.2:+20248019	None:intergenic
AACTGGTGACTGAGCTAATG+GGG	0.588096	4.2:+20247393	None:intergenic
TGCATTGACATACAATCCCA+AGG	0.588241	4.2:-20247674	MS.gene90263:CDS
AGTGATTCTGAAGCTGGCAA+TGG	0.592321	4.2:-20246579	MS.gene90263:CDS
AGGCTAAACCACAACGGTGG+TGG	0.594359	4.2:-20248027	MS.gene90263:CDS
TTGACAGTACCCAGATCCAT+AGG	0.596367	4.2:+20247769	None:intergenic
GAAGATAGATGCTGTTGATG+AGG	0.601289	4.2:-20246854	MS.gene90263:CDS
GATGCAACCCCTCCTTCTGA+GGG	0.606162	4.2:-20246889	MS.gene90263:CDS
TAACACTCCTGTAAATGCAA+CGG	0.607221	4.2:-20247830	MS.gene90263:CDS
CACTGGAACTACTACTGGCA+CGG	0.608769	4.2:+20246746	None:intergenic
GGCCAGGGATCCAAATAAGA+GGG	0.609397	4.2:-20247311	MS.gene90263:CDS
GAACCATGTTGAACCAGAGA+GGG	0.610145	4.2:-20247533	MS.gene90263:CDS
AAAGATATGGGTGGTACTGG+TGG	0.610352	4.2:-20246769	MS.gene90263:CDS
GTGGTCCCACTAAGAAATCA+GGG	0.612863	4.2:-20248008	MS.gene90263:CDS
TAAGAGGGAAATGAATGTTG+AGG	0.616464	4.2:-20247296	MS.gene90263:CDS
TGATATGGAGGCTGTTGACA+CGG	0.617215	4.2:-20247155	MS.gene90263:CDS
GGAATTGCCTGATAGGGAGA+AGG	0.617711	4.2:-20246914	MS.gene90263:CDS
ACATCAGCAGCAAAATCCGC+CGG	0.619449	4.2:+20247715	None:intergenic
TGAGGAAGCGTCATCTGATG+GGG	0.626247	4.2:+20248209	None:intergenic
GGGAGTGATTCCTCGTCGAG+TGG	0.628680	4.2:-20246709	MS.gene90263:intron
AGATGGTGAGCAAGATTAAG+CGG	0.630774	4.2:-20247090	MS.gene90263:CDS
TAAGAAATCAGGGAGCAAGA+AGG	0.642181	4.2:-20247998	MS.gene90263:CDS
CGTGCCAGTAGTAGTTCCAG+TGG	0.651947	4.2:-20246745	MS.gene90263:CDS
CTTGCTCAATCTCCACAGGT+GGG	0.652511	4.2:+20246794	None:intergenic
TGGGCATTTGGAGCAAGATG+GGG	0.655326	4.2:-20247191	MS.gene90263:CDS
GTTGATGAGGATGTTGACAT+CGG	0.656686	4.2:-20246841	MS.gene90263:CDS
TGAGATTGAGCTTGATATGG+AGG	0.664012	4.2:-20247167	MS.gene90263:CDS
GGAACCATGTTGAACCAGAG+AGG	0.667661	4.2:-20247534	MS.gene90263:CDS
GGGGATGCGATGAAGGCTTG+TGG	0.668675	4.2:-20247913	MS.gene90263:CDS
TAAGAATGCTTACTCTACGC+CGG	0.674848	4.2:-20247734	MS.gene90263:CDS
GCATTGACATACAATCCCAA+GGG	0.679665	4.2:-20247673	MS.gene90263:CDS
GAGCAAGATAAAGATATGGG+TGG	0.688901	4.2:-20246778	MS.gene90263:CDS
AATTGGCTGCAACACAGAGG+CGG	0.689298	4.2:-20248300	MS.gene90263:CDS
AATCAGGTGAGTTTAAACCC+AGG	0.697049	4.2:-20248047	MS.gene90263:CDS
GCCTTCAAACAAAGGCAATG+GGG	0.698333	4.2:-20247041	MS.gene90263:intron
ACTGGTGACTGAGCTAATGG+GGG	0.699815	4.2:+20247394	None:intergenic
GAATGCTTACTCTACGCCGG+CGG	0.711544	4.2:-20247731	MS.gene90263:CDS
CCCAGGCTAAACCACAACGG+TGG	0.730798	4.2:-20248030	MS.gene90263:CDS
CCAAATTGGCTGCAACACAG+AGG	0.734243	4.2:-20248303	MS.gene90263:CDS
AGAGGTTATACCACTCGACG+AGG	0.738536	4.2:+20246699	None:intergenic
AAACCCAGGCTAAACCACAA+CGG	0.749218	4.2:-20248033	MS.gene90263:CDS
GGGCCATGATGTTAACACTG+CGG	0.770423	4.2:-20247653	MS.gene90263:CDS
TGATGAGGATGTTGACATCG+GGG	0.772888	4.2:-20246839	MS.gene90263:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCTTTTTGTTACTAAATTTA+GGG	+	chr4.2:20246667-20246686	None:intergenic	15.0%
!!	ATCATTCAACAGAATAAAAA+TGG	+	chr4.2:20247905-20247924	None:intergenic	20.0%
!!!	CTCTTTTTGTTACTAAATTT+AGG	+	chr4.2:20246668-20246687	None:intergenic	20.0%
!	ATTGAGCAAGATAAAGATAT+GGG	-	chr4.2:20248124-20248143	MS.gene90263:CDS	25.0%
!	CAGAATAAAAATGGACAATA+AGG	+	chr4.2:20247896-20247915	None:intergenic	25.0%
!	TGATATAATTAAGCATCCTA+TGG	-	chr4.2:20247120-20247139	MS.gene90263:CDS	25.0%
!!	TGTTACTAAATTTAGGGTTA+GGG	+	chr4.2:20246661-20246680	None:intergenic	25.0%
!!	TTGTTACTAAATTTAGGGTT+AGG	+	chr4.2:20246662-20246681	None:intergenic	25.0%
!!!	AAGTTTTGATGATGAATTAC+AGG	-	chr4.2:20247339-20247358	MS.gene90263:CDS	25.0%
	AACAAGTAGTACAGATCATA+AGG	-	chr4.2:20247683-20247702	MS.gene90263:CDS	30.0%
	AAGAAATCAAAGTCAGAGAT+TGG	-	chr4.2:20246971-20246990	MS.gene90263:intron	30.0%
	AAGCTCATGAAAAACAAAAG+TGG	-	chr4.2:20247028-20247047	MS.gene90263:intron	30.0%
	AATAATGTGCCTTCAAACAA+AGG	-	chr4.2:20247856-20247875	MS.gene90263:CDS	30.0%
	ACATTCATTTCCCTCTTATT+TGG	+	chr4.2:20247607-20247626	None:intergenic	30.0%
	AGAAATCAAAGTCAGAGATT+GGG	-	chr4.2:20246972-20246991	MS.gene90263:intron	30.0%
	AGATCATAAGGAAGAGAAAT+GGG	-	chr4.2:20247695-20247714	MS.gene90263:CDS	30.0%
	GATTGAGCAAGATAAAGATA+TGG	-	chr4.2:20248123-20248142	MS.gene90263:CDS	30.0%
	GTATTGTGAATGATTATCGT+TGG	+	chr4.2:20246735-20246754	None:intergenic	30.0%
	GTTGAGGAAAAACATAAGTT+GGG	-	chr4.2:20247625-20247644	MS.gene90263:CDS	30.0%
	TCATCAAATTTCTCGTGTAT+TGG	+	chr4.2:20247316-20247335	None:intergenic	30.0%
	TCATGAAAAACAAAAGTGGT+TGG	-	chr4.2:20247032-20247051	MS.gene90263:intron	30.0%
	TGTTGAGGAAAAACATAAGT+TGG	-	chr4.2:20247624-20247643	MS.gene90263:CDS	30.0%
!	AAAAACATAAGTTGGGACTA+GGG	-	chr4.2:20247632-20247651	MS.gene90263:CDS	30.0%
!	TTTTGATCTTCATGCAGATT+CGG	-	chr4.2:20248283-20248302	MS.gene90263:CDS	30.0%
!!	AATTTGTTGTGTGCATTTAC+AGG	-	chr4.2:20247970-20247989	MS.gene90263:CDS	30.0%
!!	TTGTACTCTGAAATCATCAT+GGG	-	chr4.2:20248248-20248267	MS.gene90263:CDS	30.0%
!!	TTTGTACTCTGAAATCATCA+TGG	-	chr4.2:20248247-20248266	MS.gene90263:CDS	30.0%
	AATTAAGCATCCTATGGATC+TGG	-	chr4.2:20247126-20247145	MS.gene90263:CDS	35.0%
	ATTAAGCATCCTATGGATCT+GGG	-	chr4.2:20247127-20247146	MS.gene90263:CDS	35.0%
	ATTTACAGGAATTGCCTGAT+AGG	-	chr4.2:20247984-20248003	MS.gene90263:CDS	35.0%
	CAGATCATAAGGAAGAGAAA+TGG	-	chr4.2:20247694-20247713	MS.gene90263:CDS	35.0%
	CTGCATGAAGATCAAAATTG+AGG	+	chr4.2:20248280-20248299	None:intergenic	35.0%
	CTTAAGACTCGAATTGAATC+AGG	-	chr4.2:20246842-20246861	MS.gene90263:CDS	35.0%
	GAAATCAAAGTCAGAGATTG+GGG	-	chr4.2:20246973-20246992	MS.gene90263:intron	35.0%
	GGTTACCAATTGGAAAAAGA+TGG	-	chr4.2:20247798-20247817	MS.gene90263:CDS	35.0%
	TAACACTCCTGTAAATGCAA+CGG	-	chr4.2:20247075-20247094	MS.gene90263:CDS	35.0%
	TAAGAGGGAAATGAATGTTG+AGG	-	chr4.2:20247609-20247628	MS.gene90263:CDS	35.0%
	TTTACAGGAATTGCCTGATA+GGG	-	chr4.2:20247985-20248004	MS.gene90263:CDS	35.0%
!	CTAAATTTAGGGTTAGGGTT+AGG	+	chr4.2:20246656-20246675	None:intergenic	35.0%
!	GAAAAACATAAGTTGGGACT+AGG	-	chr4.2:20247631-20247650	MS.gene90263:CDS	35.0%
!	GTGGTTGGATTTTTAACACT+TGG	-	chr4.2:20247047-20247066	MS.gene90263:CDS	35.0%
	AAGGAAGAGAAATGGGCATT+TGG	-	chr4.2:20247702-20247721	MS.gene90263:CDS	40.0%
	AATCAGGTGAGTTTAAACCC+AGG	-	chr4.2:20246858-20246877	MS.gene90263:CDS	40.0%
	ACAACCGTAACGAACCAAAT+TGG	-	chr4.2:20246588-20246607	MS.gene90263:CDS	40.0%
	ACTACTTGTTCCATCTTCTC+TGG	+	chr4.2:20247673-20247692	None:intergenic	40.0%
	AGATGGTGAGCAAGATTAAG+CGG	-	chr4.2:20247815-20247834	MS.gene90263:CDS	40.0%
	CATCGAATCGTACACAAAGA+CGG	-	chr4.2:20246766-20246785	MS.gene90263:CDS	40.0%
	GAAGATAGATGCTGTTGATG+AGG	-	chr4.2:20248051-20248070	MS.gene90263:CDS	40.0%
	GAGCAAGATAAAGATATGGG+TGG	-	chr4.2:20248127-20248146	MS.gene90263:CDS	40.0%
	GAGTCTTAAGCTGACGAATT+TGG	+	chr4.2:20246832-20246851	None:intergenic	40.0%
	GATAAAGATATGGGTGGTAC+TGG	-	chr4.2:20248133-20248152	MS.gene90263:CDS	40.0%
	GCAGCTTCTTGAAAAATTCG+AGG	-	chr4.2:20247279-20247298	MS.gene90263:CDS	40.0%
	GCTGTCTAATGTAGCATTGT+TGG	-	chr4.2:20247928-20247947	MS.gene90263:CDS	40.0%
	GGATGAGATTGAGCTTGATA+TGG	-	chr4.2:20247735-20247754	MS.gene90263:CDS	40.0%
	GTACAAACCACAGCATTTAG+AGG	+	chr4.2:20248234-20248253	None:intergenic	40.0%
	TAAGAAATCAGGGAGCAAGA+AGG	-	chr4.2:20246907-20246926	MS.gene90263:CDS	40.0%
	TAAGAATGCTTACTCTACGC+CGG	-	chr4.2:20247171-20247190	MS.gene90263:CDS	40.0%
	TGAGATTGAGCTTGATATGG+AGG	-	chr4.2:20247738-20247757	MS.gene90263:CDS	40.0%
	TGCCTTCAAACAAAGGCAAT+GGG	-	chr4.2:20247863-20247882	MS.gene90263:CDS	40.0%
	TTATCTTGCTCAATCTCCAC+AGG	+	chr4.2:20248118-20248137	None:intergenic	40.0%
	TTCAAAGCCGTTGCATTTAC+AGG	+	chr4.2:20247085-20247104	None:intergenic	40.0%
	TTCATGCAGATTCGGATTCA+GGG	-	chr4.2:20248291-20248310	MS.gene90263:CDS	40.0%
	TTGATGAGGATGTTGACATC+GGG	-	chr4.2:20248065-20248084	MS.gene90263:CDS	40.0%
	TTGCTCCCTGATTTCTTAGT+GGG	+	chr4.2:20246905-20246924	None:intergenic	40.0%
!	GCATTGACATACAATCCCAA+GGG	-	chr4.2:20247232-20247251	MS.gene90263:CDS	40.0%
!	GCTCACCATCTTTTTCCAAT+TGG	+	chr4.2:20247806-20247825	None:intergenic	40.0%
!	GTTGATGAGGATGTTGACAT+CGG	-	chr4.2:20248064-20248083	MS.gene90263:CDS	40.0%
!	TCGATCGATTGGTTACCAAT+TGG	-	chr4.2:20247788-20247807	MS.gene90263:CDS	40.0%
!	TGCATTGACATACAATCCCA+AGG	-	chr4.2:20247231-20247250	MS.gene90263:CDS	40.0%
!	TTCTGTTTCTTTCCCTCAGA+AGG	+	chr4.2:20248031-20248050	None:intergenic	40.0%
	AAACCCAGGCTAAACCACAA+CGG	-	chr4.2:20246872-20246891	MS.gene90263:CDS	45.0%
	AACTGGTGACTGAGCTAATG+GGG	+	chr4.2:20247515-20247534	None:intergenic	45.0%
	AATTCCTTCACCGCAGGAAA+AGG	+	chr4.2:20246950-20246969	None:intergenic	45.0%
	ACTTGTTCCATCTTCTCTGG+TGG	+	chr4.2:20247670-20247689	None:intergenic	45.0%
	AGTGTTAACATCATGGCCCT+TGG	+	chr4.2:20247251-20247270	None:intergenic	45.0%
	ATGTTGAACCAGAGAGGGAA+AGG	-	chr4.2:20247377-20247396	MS.gene90263:CDS	45.0%
	CCACAGCATTTAGAGGAAAG+AGG	+	chr4.2:20248227-20248246	None:intergenic	45.0%
	CCTCTTTCCTCTAAATGCTG+TGG	-	chr4.2:20248224-20248243	MS.gene90263:CDS	45.0%
	CTTCATGCAGATTCGGATTC+AGG	-	chr4.2:20248290-20248309	MS.gene90263:CDS	45.0%
	CTTGCTCCCTGATTTCTTAG+TGG	+	chr4.2:20246906-20246925	None:intergenic	45.0%
	GAACCATGTTGAACCAGAGA+GGG	-	chr4.2:20247372-20247391	MS.gene90263:CDS	45.0%
	GAACTGGTGACTGAGCTAAT+GGG	+	chr4.2:20247516-20247535	None:intergenic	45.0%
	GCCTTCAAACAAAGGCAATG+GGG	-	chr4.2:20247864-20247883	MS.gene90263:CDS	45.0%
	GTGCCTTCAAACAAAGGCAA+TGG	-	chr4.2:20247862-20247881	MS.gene90263:CDS	45.0%
	GTGGTCCCACTAAGAAATCA+GGG	-	chr4.2:20246897-20246916	MS.gene90263:CDS	45.0%
	GTGTTAACATCATGGCCCTT+GGG	+	chr4.2:20247250-20247269	None:intergenic	45.0%
	GTTTCTTTCCCTCAGAAGGA+GGG	+	chr4.2:20248027-20248046	None:intergenic	45.0%
	TGATATGGAGGCTGTTGACA+CGG	-	chr4.2:20247750-20247769	MS.gene90263:CDS	45.0%
	TGATGAGGATGTTGACATCG+GGG	-	chr4.2:20248066-20248085	MS.gene90263:CDS	45.0%
	TGTTGAACCAGAGAGGGAAA+GGG	-	chr4.2:20247378-20247397	MS.gene90263:CDS	45.0%
	TGTTTCTTTCCCTCAGAAGG+AGG	+	chr4.2:20248028-20248047	None:intergenic	45.0%
	TTCTTCAATTCCTTCACCGC+AGG	+	chr4.2:20246956-20246975	None:intergenic	45.0%
	TTGACAGTACCCAGATCCAT+AGG	+	chr4.2:20247139-20247158	None:intergenic	45.0%
	TTTCTTTCCCTCAGAAGGAG+GGG	+	chr4.2:20248026-20248045	None:intergenic	45.0%
!	AAAGATATGGGTGGTACTGG+TGG	-	chr4.2:20248136-20248155	MS.gene90263:CDS	45.0%
!	AATGGGCATTTGGAGCAAGA+TGG	-	chr4.2:20247712-20247731	MS.gene90263:CDS	45.0%
!	AATGGGGGATTCAAAGTGCT+TGG	+	chr4.2:20247499-20247518	None:intergenic	45.0%
!	ACCCCATTGCCTTTGTTTGA+AGG	+	chr4.2:20247868-20247887	None:intergenic	45.0%
!	AGGAAAAGGTCGTTTGTTGC+CGG	+	chr4.2:20246936-20246955	None:intergenic	45.0%
!	ATGAATTACAGGCTAGCTCG+TGG	-	chr4.2:20247350-20247369	MS.gene90263:CDS	45.0%
!	ATGGGCATTTGGAGCAAGAT+GGG	-	chr4.2:20247713-20247732	MS.gene90263:CDS	45.0%
!	CAACAAACGACCTTTTCCTG+CGG	-	chr4.2:20246937-20246956	MS.gene90263:intron	45.0%
!	GATTTTGCCACCAGAGAAGA+TGG	-	chr4.2:20247660-20247679	MS.gene90263:CDS	45.0%
!	GCAGCCAATTTGGTTCGTTA+CGG	+	chr4.2:20246595-20246614	None:intergenic	45.0%
!	GTTGGATCGTCGATTGATTG+AGG	+	chr4.2:20246717-20246736	None:intergenic	45.0%
!	TTATTTGGATCCCTGGCCTT+TGG	+	chr4.2:20247592-20247611	None:intergenic	45.0%
!	TTCCCTCTTATTTGGATCCC+TGG	+	chr4.2:20247599-20247618	None:intergenic	45.0%
!	TTTCCCTCTCTGGTTCAACA+TGG	+	chr4.2:20247378-20247397	None:intergenic	45.0%
!!	AGTGATTCTGAAGCTGGCAA+TGG	-	chr4.2:20248326-20248345	MS.gene90263:CDS	45.0%
!!	ATTGAGGAAGCGTCATCTGA+TGG	+	chr4.2:20246701-20246720	None:intergenic	45.0%
!!	TCAGGAAGTGATTCTGAAGC+TGG	-	chr4.2:20248320-20248339	MS.gene90263:CDS	45.0%
!!	TTAGAAGTACTAGCAGGCTC+AGG	+	chr4.2:20247472-20247491	None:intergenic	45.0%
!!	TTGAGGAAGCGTCATCTGAT+GGG	+	chr4.2:20246700-20246719	None:intergenic	45.0%
	AATTGGCTGCAACACAGAGG+CGG	-	chr4.2:20246605-20246624	MS.gene90263:CDS	50.0%
	ACAGGTGGGAAATCGTTTGC+TGG	+	chr4.2:20248100-20248119	None:intergenic	50.0%
	ACATCAGCAGCAAAATCCGC+CGG	+	chr4.2:20247193-20247212	None:intergenic	50.0%
	ACTGGTGACTGAGCTAATGG+GGG	+	chr4.2:20247514-20247533	None:intergenic	50.0%
	AGAGATTGGGGATGCGATGA+AGG	-	chr4.2:20246985-20247004	MS.gene90263:intron	50.0%
	AGAGGTTATACCACTCGACG+AGG	+	chr4.2:20248209-20248228	None:intergenic	50.0%
	AGCAAACGATTTCCCACCTG+TGG	-	chr4.2:20248099-20248118	MS.gene90263:CDS	50.0%
	AGGCCAGGGATCCAAATAAG+AGG	-	chr4.2:20247593-20247612	MS.gene90263:CDS	50.0%
	CACTGGAACTACTACTGGCA+CGG	+	chr4.2:20248162-20248181	None:intergenic	50.0%
	CCAAATTGGCTGCAACACAG+AGG	-	chr4.2:20246602-20246621	MS.gene90263:CDS	50.0%
	CCTCTGTGTTGCAGCCAATT+TGG	+	chr4.2:20246605-20246624	None:intergenic	50.0%
	CGAACTGGTGACTGAGCTAA+TGG	+	chr4.2:20247517-20247536	None:intergenic	50.0%
	CTTGCTCAATCTCCACAGGT+GGG	+	chr4.2:20248114-20248133	None:intergenic	50.0%
	GCATCAACCTTCTCCCTATC+AGG	+	chr4.2:20248001-20248020	None:intergenic	50.0%
	GGAACCACTGGAACTACTAC+TGG	+	chr4.2:20248167-20248186	None:intergenic	50.0%
	GGAACCATGTTGAACCAGAG+AGG	-	chr4.2:20247371-20247390	MS.gene90263:CDS	50.0%
	GGAATTGCCTGATAGGGAGA+AGG	-	chr4.2:20247991-20248010	MS.gene90263:CDS	50.0%
	GGCCAGGGATCCAAATAAGA+GGG	-	chr4.2:20247594-20247613	MS.gene90263:CDS	50.0%
	GGGCCATGATGTTAACACTG+CGG	-	chr4.2:20247252-20247271	MS.gene90263:CDS	50.0%
	GGTGGTCCCACTAAGAAATC+AGG	-	chr4.2:20246896-20246915	MS.gene90263:CDS	50.0%
	TCAGGGAGCAAGAAGGTTTC+CGG	-	chr4.2:20246914-20246933	MS.gene90263:CDS	50.0%
	TCCAATTCCAATCCCTCCAC+CGG	-	chr4.2:20247423-20247442	MS.gene90263:CDS	50.0%
	TCGCCGCAGTGTTAACATCA+TGG	+	chr4.2:20247258-20247277	None:intergenic	50.0%
	TCGGATTCAGGGAGTTCTTC+AGG	-	chr4.2:20248302-20248321	MS.gene90263:CDS	50.0%
	TCTTGCTCAATCTCCACAGG+TGG	+	chr4.2:20248115-20248134	None:intergenic	50.0%
	TGTTGACACGGAGACTCTCT+GGG	-	chr4.2:20247762-20247781	MS.gene90263:CDS	50.0%
!	TCTCTGGGAACTCGATCGAT+TGG	-	chr4.2:20247777-20247796	MS.gene90263:CDS	50.0%
!	TGGGCATTTGGAGCAAGATG+GGG	-	chr4.2:20247714-20247733	MS.gene90263:CDS	50.0%
!!	GGCTGGTTAGAAGTACTAGC+AGG	+	chr4.2:20247478-20247497	None:intergenic	50.0%
!!	TGAGGAAGCGTCATCTGATG+GGG	+	chr4.2:20246699-20246718	None:intergenic	50.0%
!!	TTTGGCTTGGGCTGCTTCAA+GGG	+	chr4.2:20247574-20247593	None:intergenic	50.0%
	AACCAGAGAGGGAAAGGGAG+AGG	-	chr4.2:20247383-20247402	MS.gene90263:CDS	55.0%
	ACCACCGTTGTGGTTTAGCC+TGG	+	chr4.2:20246879-20246898	None:intergenic	55.0%
	ACCAGAGAGGGAAAGGGAGA+GGG	-	chr4.2:20247384-20247403	MS.gene90263:CDS	55.0%
	ACCGGTGGAGGGATTGGAAT+TGG	+	chr4.2:20247427-20247446	None:intergenic	55.0%
	AGGCGGTGGAGCTGAATTCA+TGG	-	chr4.2:20246622-20246641	MS.gene90263:intron	55.0%
	AGGCTAAACCACAACGGTGG+TGG	-	chr4.2:20246878-20246897	MS.gene90263:CDS	55.0%
	CCACCGTTGTGGTTTAGCCT+GGG	+	chr4.2:20246878-20246897	None:intergenic	55.0%
	CGTGCCAGTAGTAGTTCCAG+TGG	-	chr4.2:20248160-20248179	MS.gene90263:CDS	55.0%
	CTGTTGACACGGAGACTCTC+TGG	-	chr4.2:20247761-20247780	MS.gene90263:CDS	55.0%
	CTTGAAGCAGCCCAAGCCAA+AGG	-	chr4.2:20247573-20247592	MS.gene90263:CDS	55.0%
	GAATGCTTACTCTACGCCGG+CGG	-	chr4.2:20247174-20247193	MS.gene90263:CDS	55.0%
	GATGCAACCCCTCCTTCTGA+GGG	-	chr4.2:20248016-20248035	MS.gene90263:CDS	55.0%
	GATTAAGCGGCAGGCGCTAA+TGG	-	chr4.2:20247828-20247847	MS.gene90263:CDS	55.0%
	GGCGGTGGAGCTGAATTCAT+GGG	-	chr4.2:20246623-20246642	MS.gene90263:intron	55.0%
	GGTGAGCAAGATTAAGCGGC+AGG	-	chr4.2:20247819-20247838	MS.gene90263:CDS	55.0%
	TGATGCAACCCCTCCTTCTG+AGG	-	chr4.2:20248015-20248034	MS.gene90263:CDS	55.0%
	TTTGCTACCGGTGGAGGGAT+TGG	+	chr4.2:20247433-20247452	None:intergenic	55.0%
!	ACGACCTTTTCCTGCGGTGA+AGG	-	chr4.2:20246943-20246962	MS.gene90263:intron	55.0%
!	GGCTCTTGGCGTTTTGCTAC+CGG	+	chr4.2:20247445-20247464	None:intergenic	55.0%
!	GGGGATTCAAAGTGCTTGGC+TGG	+	chr4.2:20247495-20247514	None:intergenic	55.0%
!	TCTTGGCGTTTTGCTACCGG+TGG	+	chr4.2:20247442-20247461	None:intergenic	55.0%
!!	CTTTGGCTTGGGCTGCTTCA+AGG	+	chr4.2:20247575-20247594	None:intergenic	55.0%
!!	TCACTGGAAGTGCTCGCGTT+GGG	+	chr4.2:20247549-20247568	None:intergenic	55.0%
!!	TTCACTGGAAGTGCTCGCGT+TGG	+	chr4.2:20247550-20247569	None:intergenic	55.0%
	ACCCTCTCCCTTTCCCTCTC+TGG	+	chr4.2:20247388-20247407	None:intergenic	60.0%
	CCAGTGGTTCCAGCAGCTCT+GGG	-	chr4.2:20248176-20248195	MS.gene90263:CDS	60.0%
	CCCAGGCTAAACCACAACGG+TGG	-	chr4.2:20246875-20246894	MS.gene90263:CDS	60.0%
	GGAATCACTCCCAGAGCTGC+TGG	+	chr4.2:20248188-20248207	None:intergenic	60.0%
	GGCTGCTTCAAGGGCTTCAC+TGG	+	chr4.2:20247565-20247584	None:intergenic	60.0%
	GGGAGTGATTCCTCGTCGAG+TGG	-	chr4.2:20248196-20248215	MS.gene90263:CDS	60.0%
	GGGGATGCGATGAAGGCTTG+TGG	-	chr4.2:20246992-20247011	MS.gene90263:intron	60.0%
	GTACTAGCAGGCTCAGGCAG+TGG	+	chr4.2:20247466-20247485	None:intergenic	60.0%
	TAGTGGGACCACCACCGTTG+TGG	+	chr4.2:20246889-20246908	None:intergenic	60.0%
	TCCAGTGGTTCCAGCAGCTC+TGG	-	chr4.2:20248175-20248194	MS.gene90263:CDS	60.0%
!	GGATCCCTGGCCTTTGGCTT+GGG	+	chr4.2:20247586-20247605	None:intergenic	60.0%
!	GGCGTTTTGCTACCGGTGGA+GGG	+	chr4.2:20247438-20247457	None:intergenic	60.0%
!	GTTGGGGAGGGTATGCGAAC+TGG	+	chr4.2:20247532-20247551	None:intergenic	60.0%
!	TGGATCCCTGGCCTTTGGCT+TGG	+	chr4.2:20247587-20247606	None:intergenic	60.0%
!	TGGCGTTTTGCTACCGGTGG+AGG	+	chr4.2:20247439-20247458	None:intergenic	60.0%
!!	CACTGGAAGTGCTCGCGTTG+GGG	+	chr4.2:20247548-20247567	None:intergenic	60.0%
	AGCAGCCCAAGCCAAAGGCC+AGG	-	chr4.2:20247578-20247597	MS.gene90263:CDS	65.0%
	CAGGCTCAGGCAGTGGCTCT+TGG	+	chr4.2:20247459-20247478	None:intergenic	65.0%
	CCCAGAGCTGCTGGAACCAC+TGG	+	chr4.2:20248179-20248198	None:intergenic	65.0%
	GCAGCCCAAGCCAAAGGCCA+GGG	-	chr4.2:20247579-20247598	MS.gene90263:CDS	65.0%
	TGGCTGCAACACAGAGGCGG+TGG	-	chr4.2:20246608-20246627	MS.gene90263:CDS	65.0%
!!	GGAAGTGCTCGCGTTGGGGA+GGG	+	chr4.2:20247544-20247563	None:intergenic	65.0%
!!	TGGAAGTGCTCGCGTTGGGG+AGG	+	chr4.2:20247545-20247564	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	20246569	20248358	20246569	ID=MS.gene90263
chr4.2	mRNA	20246569	20248358	20246569	ID=MS.gene90263.t1;Parent=MS.gene90263
chr4.2	exon	20247042	20248358	20247042	ID=MS.gene90263.t1.exon1;Parent=MS.gene90263.t1
chr4.2	CDS	20247042	20248358	20247042	ID=cds.MS.gene90263.t1;Parent=MS.gene90263.t1
chr4.2	exon	20246710	20246935	20246710	ID=MS.gene90263.t1.exon2;Parent=MS.gene90263.t1
chr4.2	CDS	20246710	20246935	20246710	ID=cds.MS.gene90263.t1;Parent=MS.gene90263.t1
chr4.2	exon	20246569	20246627	20246569	ID=MS.gene90263.t1.exon3;Parent=MS.gene90263.t1
chr4.2	CDS	20246569	20246627	20246569	ID=cds.MS.gene90263.t1;Parent=MS.gene90263.t1

Gene Sequence

>MS.gene90263

ATGGCATCGGCTTTATTAGACAACCGTAACGAACCAAATTGGCTGCAACACAGAGGCGGTGGAGCTGAATTCATGGGTAAAGCACCTAACCCTAACCCTAAATTTAGTAACAAAAAGAGAACTCAATCCCCATCAGATGACGCTTCCTCAATCAATCGACGATCCAACGATAATCATTCACAATACGTTACTTTCAACATCGAATCGTACACAAAGACGGAGCTATACGAGCTCAAAAATCGACTCGTATCAGAACTCGACCAAATTCGTCAGCTTAAGACTCGAATTGAATCAGGTGAGTTTAAACCCAGGCTAAACCACAACGGTGGTGGTCCCACTAAGAAATCAGGGAGCAAGAAGGTTTCCGGCAACAAACGACCTTTTCCTGCGGTGAAGGAATTGAAGAAATCAAAGTCAGAGATTGGGGATGCGATGAAGGCTTGTGGTCAGATTTTGACGAAGCTCATGAAAAACAAAAGTGGTTGGATTTTTAACACTTGGATCTTTAACACTCCTGTAAATGCAACGGCTTTGAATCTTCATGATTACTTTGATATAATTAAGCATCCTATGGATCTGGGTACTGTCAAGTCAAAACTTGCTAAGAATGCTTACTCTACGCCGGCGGATTTTGCTGCTGATGTGAAGTTAACGTTCAACAATGCATTGACATACAATCCCAAGGGCCATGATGTTAACACTGCGGCGAAGCAGCTTCTTGAAAAATTCGAGGAGCTTTATCGACCAATACACGAGAAATTTGATGAAAAAAGTTTTGATGATGAATTACAGGCTAGCTCGTGGAACCATGTTGAACCAGAGAGGGAAAGGGAGAGGGTGAAGAAGAAAGATAATCCAATTCCAATCCCTCCACCGGTAGCAAAACGCCAAGAGCCACTGCCTGAGCCTGCTAGTACTTCTAACCAGCCAAGCACTTTGAATCCCCCATTAGCTCAGTCACCAGTTCGCATACCCTCCCCAACGCGAGCACTTCCAGTGAAGCCCTTGAAGCAGCCCAAGCCAAAGGCCAGGGATCCAAATAAGAGGGAAATGAATGTTGAGGAAAAACATAAGTTGGGACTAGGGTTGCAGATTTTGCCACCAGAGAAGATGGAACAAGTAGTACAGATCATAAGGAAGAGAAATGGGCATTTGGAGCAAGATGGGGATGAGATTGAGCTTGATATGGAGGCTGTTGACACGGAGACTCTCTGGGAACTCGATCGATTGGTTACCAATTGGAAAAAGATGGTGAGCAAGATTAAGCGGCAGGCGCTAATGGATAATAATAATGTGCCTTCAAACAAAGGCAATGGGGTAAAAACCTTATTGTCCATTTTTATTCTGTTGAATGATGTTGCTGTCTAATGTAGCATTGTTGGCAGTGTTTTATCACATTCTAATTTGTTGTGTGCATTTACAGGAATTGCCTGATAGGGAGAAGGTTGATGCAACCCCTCCTTCTGAGGGAAAGAAACAGAAGAAGATAGATGCTGTTGATGAGGATGTTGACATCGGGGATGATATGCCAGCAAACGATTTCCCACCTGTGGAGATTGAGCAAGATAAAGATATGGGTGGTACTGGTGGCCGTGCCAGTAGTAGTTCCAGTGGTTCCAGCAGCTCTGGGAGTGATTCCTCGTCGAGTGGTATAACCTCTTTCCTCTAAATGCTGTGGTTTGTACTCTGAAATCATCATGGGTAGAAACCTCAATTTTGATCTTCATGCAGATTCGGATTCAGGGAGTTCTTCAGGAAGTGATTCTGAAGCTGGCAATGGTCATTTGTAG

Protein sequence

>MS.gene90263.t1

MASALLDNRNEPNWLQHRGGGAEFMGKAPNPNPKFSNKKRTQSPSDDASSINRRSNDNHSQYVTFNIESYTKTELYELKNRLVSELDQIRQLKTRIESGEFKPRLNHNGGGPTKKSGSKKVSGNKRPFPAVKELKKSKSEIGDAMKACGQILTKLMKNKSGWIFNTWIFNTPVNATALNLHDYFDIIKHPMDLGTVKSKLAKNAYSTPADFAADVKLTFNNALTYNPKGHDVNTAAKQLLEKFEELYRPIHEKFDEKSFDDELQASSWNHVEPERERERVKKKDNPIPIPPPVAKRQEPLPEPASTSNQPSTLNPPLAQSPVRIPSPTRALPVKPLKQPKPKARDPNKREMNVEEKHKLGLGLQILPPEKMEQVVQIIRKRNGHLEQDGDEIELDMEAVDTETLWELDRLVTNWKKMVSKIKRQALMDNNNVPSNKGNGELPDREKVDATPPSEGKKQKKIDAVDEDVDIGDDMPANDFPPVEIEQDKDMGGTGGRASSSSSGSSSSGSDSSSSDSDSGSSSGSDSEAGNGHL