AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80201


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80201.t1 AT2G32070 78.030 264 57 1 10 272 6 269 8.23e-155 432
MS.gene80201.t1 AT1G80780 77.567 263 59 0 10 272 6 268 6.29e-154 430
MS.gene80201.t1 AT1G80780 77.567 263 59 0 10 272 6 268 6.29e-154 430
MS.gene80201.t1 AT1G80780 77.567 263 59 0 10 272 6 268 6.67e-154 431
MS.gene80201.t1 AT5G10960 76.806 263 61 0 10 272 6 268 6.14e-153 428
MS.gene80201.t1 AT1G15920 61.091 275 101 1 1 269 1 275 9.35e-120 344
MS.gene80201.t1 AT1G15920 61.091 275 101 1 1 269 1 275 9.35e-120 344
MS.gene80201.t1 AT3G44260 53.846 273 118 7 6 271 8 279 2.09e-92 275
MS.gene80201.t1 AT5G22250 52.985 268 118 6 12 271 10 277 4.12e-89 266
MS.gene80201.t1 AT1G06450 36.680 259 146 7 17 270 10 255 2.38e-45 156
MS.gene80201.t1 AT1G61470 35.743 249 140 6 18 260 4 238 3.56e-37 133
MS.gene80201.t1 AT3G44240 37.719 228 128 6 30 254 3 219 9.04e-36 128
MS.gene80201.t1 AT1G27820 32.806 253 154 6 18 265 8 249 4.39e-31 117
MS.gene80201.t1 AT1G27890 33.468 248 149 6 18 260 3 239 1.80e-28 110

Find 50 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCACAGTGGTTATGATTT+TGG 0.162019 3.4:+92608239 MS.gene80201:CDS
TACTCACAGCTTGTACATTT+AGG 0.316594 3.4:+92608468 MS.gene80201:CDS
GGAATCGACGTGAACAGATT+TGG 0.350401 3.4:+92608161 MS.gene80201:CDS
GAACAAGCTTGCGGAATTGC+TGG 0.383855 3.4:+92608400 MS.gene80201:CDS
AGAGGTATGGAACGATAATC+TGG 0.393193 3.4:+92607797 MS.gene80201:CDS
TTTGACGTGCAGGGCTTTGC+CGG 0.395764 3.4:+92608277 MS.gene80201:CDS
AGTTTCCTGGTGTTGTTCTT+CGG 0.398208 3.4:+92607883 MS.gene80201:CDS
GGCGAAACAGAGAAATATTC+TGG 0.400040 3.4:+92608515 MS.gene80201:CDS
TATACTTATCAACAATTTCA+CGG 0.412664 3.4:-92607839 None:intergenic
AACTATCCGATCCAGCTTGA+TGG 0.415822 3.4:-92608445 None:intergenic
TCTGGTGTGTTGTATGGATT+AGG 0.418411 3.4:+92608533 MS.gene80201:CDS
AAGTTCTGTAACAGTCTTCA+TGG 0.419529 3.4:+92608371 MS.gene80201:CDS
GGTGTTGTTCTTCGGCCTGT+TGG 0.419609 3.4:+92607891 MS.gene80201:CDS
AAATATTCTGGTGTGTTGTA+TGG 0.424598 3.4:+92608527 MS.gene80201:CDS
GGAGAACTTCTGATGTCATC+CGG 0.426593 3.4:+92608182 MS.gene80201:CDS
GTGTTGTTCTTCGGCCTGTT+GGG 0.429390 3.4:+92607892 MS.gene80201:CDS
CGGAATTGCTGGAAGTTGAG+AGG 0.432657 3.4:+92608411 MS.gene80201:CDS
ATGTTGAAGCTGATTCAGTT+AGG 0.437086 3.4:+92607969 MS.gene80201:CDS
TGTTGAATGATAATGTTCAT+TGG 0.444197 3.4:+92608210 MS.gene80201:CDS
ATATGCTTAAAATTCCCAAC+AGG 0.468744 3.4:-92607906 None:intergenic
TGTTTGATATCATACACAAT+CGG 0.482779 3.4:-92608341 None:intergenic
TTGTACATTTAGGAAGTTGA+GGG 0.486839 3.4:+92608478 MS.gene80201:CDS
TTAACGAAGGATTTACAGAA+AGG 0.493060 3.4:+92607756 MS.gene80201:CDS
GTTGAATGATAATGTTCATT+GGG 0.498652 3.4:+92608211 MS.gene80201:CDS
GTTTGATATCATACACAATC+GGG 0.509746 3.4:-92608340 None:intergenic
GCACTGATAGTCCTTGCATC+TGG 0.511115 3.4:+92608033 MS.gene80201:CDS
CGATTCAAATCAGAGAGGTA+TGG 0.516845 3.4:+92607784 MS.gene80201:CDS
GTTGGTGTGTGCCATCAAGC+TGG 0.516903 3.4:+92608434 MS.gene80201:CDS
TCTTAAAATCAATTCCGCAT+TGG 0.519845 3.4:-92608124 None:intergenic
GCGATGGATACAGAGTTTCC+TGG 0.522554 3.4:+92607870 MS.gene80201:CDS
GAGAACTTCTGATGTCATCC+GGG 0.551352 3.4:+92608183 MS.gene80201:CDS
TTTAAGAAGAACAGCGAGCA+AGG 0.554251 3.4:+92608140 MS.gene80201:CDS
CTTGTACATTTAGGAAGTTG+AGG 0.555789 3.4:+92608477 MS.gene80201:CDS
GCTTTGCCGGATACACAAGC+TGG 0.557400 3.4:+92608290 MS.gene80201:CDS
ATGGAACGATAATCTGGAAG+AGG 0.557973 3.4:+92607803 MS.gene80201:CDS
GCAAGGACTATCAGTGCCAC+AGG 0.564639 3.4:-92608027 None:intergenic
TGGTGGTTTGAACAAGCTTG+CGG 0.579595 3.4:+92608391 MS.gene80201:CDS
AAATTGAATTGCCAGATGCA+AGG 0.592828 3.4:-92608044 None:intergenic
TAAGTATAACTATGTTGCGA+TGG 0.597473 3.4:+92607854 MS.gene80201:CDS
TTGCTGGAAGTTGAGAGGGT+TGG 0.602040 3.4:+92608416 MS.gene80201:CDS
ATTATCATTCAACACAATCC+CGG 0.602210 3.4:-92608201 None:intergenic
TTGAGGGAAACATTCTTCAA+CGG 0.604445 3.4:+92608494 MS.gene80201:CDS
ACAGGCCGAAGAACAACACC+AGG 0.618032 3.4:-92607888 None:intergenic
GGAATTGCTGGAAGTTGAGA+GGG 0.625969 3.4:+92608412 MS.gene80201:CDS
GGACTATCAGTGCCACAGGT+AGG 0.632411 3.4:-92608023 None:intergenic
TGAAATGACGGATTTAACGA+AGG 0.651784 3.4:+92607743 None:intergenic
TGTGTGCCATCAAGCTGGAT+CGG 0.653723 3.4:+92608439 MS.gene80201:CDS
TTCTGTAACAGTCTTCATGG+TGG 0.661335 3.4:+92608374 MS.gene80201:CDS
AATGGAAATTTACCTACCTG+TGG 0.695151 3.4:+92608011 MS.gene80201:CDS
AGATTCGATTCAAATCAGAG+AGG 0.775869 3.4:+92607779 MS.gene80201:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATACTTATCAACAATTTCA+CGG - chr3.4:92607842-92607861 None:intergenic 20.0%
! TGTTTGATATCATACACAAT+CGG - chr3.4:92608344-92608363 None:intergenic 25.0%
!! GTTGAATGATAATGTTCATT+GGG + chr3.4:92608211-92608230 MS.gene80201:CDS 25.0%
!! TGTTGAATGATAATGTTCAT+TGG + chr3.4:92608210-92608229 MS.gene80201:CDS 25.0%
!!! TTAACTTTTTCTGATGAGAA+TGG + chr3.4:92607993-92608012 MS.gene80201:CDS 25.0%
!!! TTTTAACTATCAGACTTTGA+AGG + chr3.4:92607935-92607954 MS.gene80201:CDS 25.0%
AAATATTCTGGTGTGTTGTA+TGG + chr3.4:92608527-92608546 MS.gene80201:CDS 30.0%
ATATGCTTAAAATTCCCAAC+AGG - chr3.4:92607909-92607928 None:intergenic 30.0%
ATTATCATTCAACACAATCC+CGG - chr3.4:92608204-92608223 None:intergenic 30.0%
GTTTGATATCATACACAATC+GGG - chr3.4:92608343-92608362 None:intergenic 30.0%
TAAGTATAACTATGTTGCGA+TGG + chr3.4:92607854-92607873 MS.gene80201:CDS 30.0%
TCTTAAAATCAATTCCGCAT+TGG - chr3.4:92608127-92608146 None:intergenic 30.0%
TTAACGAAGGATTTACAGAA+AGG + chr3.4:92607756-92607775 MS.gene80201:CDS 30.0%
TTGTACATTTAGGAAGTTGA+GGG + chr3.4:92608478-92608497 MS.gene80201:CDS 30.0%
! TTTCACAGTGGTTATGATTT+TGG + chr3.4:92608239-92608258 MS.gene80201:CDS 30.0%
AAAAATCCAGCTTGTGTATC+CGG - chr3.4:92608299-92608318 None:intergenic 35.0%
AAATTGAATTGCCAGATGCA+AGG - chr3.4:92608047-92608066 None:intergenic 35.0%
AAGTTCTGTAACAGTCTTCA+TGG + chr3.4:92608371-92608390 MS.gene80201:CDS 35.0%
AATGGAAATTTACCTACCTG+TGG + chr3.4:92608011-92608030 MS.gene80201:CDS 35.0%
AGATTCGATTCAAATCAGAG+AGG + chr3.4:92607779-92607798 MS.gene80201:CDS 35.0%
CTTGTACATTTAGGAAGTTG+AGG + chr3.4:92608477-92608496 MS.gene80201:CDS 35.0%
TACTCACAGCTTGTACATTT+AGG + chr3.4:92608468-92608487 MS.gene80201:CDS 35.0%
TTGAGGGAAACATTCTTCAA+CGG + chr3.4:92608494-92608513 MS.gene80201:CDS 35.0%
! ATGTTGAAGCTGATTCAGTT+AGG + chr3.4:92607969-92607988 MS.gene80201:CDS 35.0%
!! CTGGATTTTTCGATTTGATC+GGG + chr3.4:92608309-92608328 MS.gene80201:CDS 35.0%
!! GCTGGATTTTTCGATTTGAT+CGG + chr3.4:92608308-92608327 MS.gene80201:CDS 35.0%
ATGGAACGATAATCTGGAAG+AGG + chr3.4:92607803-92607822 MS.gene80201:CDS 40.0%
CGATTCAAATCAGAGAGGTA+TGG + chr3.4:92607784-92607803 MS.gene80201:CDS 40.0%
GGCGAAACAGAGAAATATTC+TGG + chr3.4:92608515-92608534 MS.gene80201:CDS 40.0%
TCTGGTGTGTTGTATGGATT+AGG + chr3.4:92608533-92608552 MS.gene80201:CDS 40.0%
TTCTGTAACAGTCTTCATGG+TGG + chr3.4:92608374-92608393 MS.gene80201:CDS 40.0%
TTTAAGAAGAACAGCGAGCA+AGG + chr3.4:92608140-92608159 MS.gene80201:CDS 40.0%
! AGAGGTATGGAACGATAATC+TGG + chr3.4:92607797-92607816 MS.gene80201:CDS 40.0%
!! AGTTTCCTGGTGTTGTTCTT+CGG + chr3.4:92607883-92607902 MS.gene80201:CDS 40.0%
!! TTTGAAGCTTTTGACGTGCA+GGG + chr3.4:92608268-92608287 MS.gene80201:CDS 40.0%
!!! CATTGGGTTACTTTTCACAG+TGG + chr3.4:92608227-92608246 MS.gene80201:CDS 40.0%
!!! TTTTGAAGCTTTTGACGTGC+AGG + chr3.4:92608267-92608286 MS.gene80201:CDS 40.0%
AACTATCCGATCCAGCTTGA+TGG - chr3.4:92608448-92608467 None:intergenic 45.0%
GAGAACTTCTGATGTCATCC+GGG + chr3.4:92608183-92608202 MS.gene80201:CDS 45.0%
GGAATCGACGTGAACAGATT+TGG + chr3.4:92608161-92608180 MS.gene80201:CDS 45.0%
GGAATTGCTGGAAGTTGAGA+GGG + chr3.4:92608412-92608431 MS.gene80201:CDS 45.0%
GGAGAACTTCTGATGTCATC+CGG + chr3.4:92608182-92608201 MS.gene80201:CDS 45.0%
TGGTGGTTTGAACAAGCTTG+CGG + chr3.4:92608391-92608410 MS.gene80201:CDS 45.0%
CGGAATTGCTGGAAGTTGAG+AGG + chr3.4:92608411-92608430 MS.gene80201:CDS 50.0%
GAACAAGCTTGCGGAATTGC+TGG + chr3.4:92608400-92608419 MS.gene80201:CDS 50.0%
GCGATGGATACAGAGTTTCC+TGG + chr3.4:92607870-92607889 MS.gene80201:CDS 50.0%
TGTGTGCCATCAAGCTGGAT+CGG + chr3.4:92608439-92608458 MS.gene80201:CDS 50.0%
TTGCTGGAAGTTGAGAGGGT+TGG + chr3.4:92608416-92608435 MS.gene80201:CDS 50.0%
! ATCGAGCTTTTGCGCCAATG+CGG + chr3.4:92608110-92608129 MS.gene80201:CDS 50.0%
! GCACTGATAGTCCTTGCATC+TGG + chr3.4:92608033-92608052 MS.gene80201:CDS 50.0%
! GTGTTGTTCTTCGGCCTGTT+GGG + chr3.4:92607892-92607911 MS.gene80201:CDS 50.0%
ACAGGCCGAAGAACAACACC+AGG - chr3.4:92607891-92607910 None:intergenic 55.0%
GCAAGGACTATCAGTGCCAC+AGG - chr3.4:92608030-92608049 None:intergenic 55.0%
GCTTTGCCGGATACACAAGC+TGG + chr3.4:92608290-92608309 MS.gene80201:CDS 55.0%
GGACTATCAGTGCCACAGGT+AGG - chr3.4:92608026-92608045 None:intergenic 55.0%
GTTGGTGTGTGCCATCAAGC+TGG + chr3.4:92608434-92608453 MS.gene80201:CDS 55.0%
TTTGACGTGCAGGGCTTTGC+CGG + chr3.4:92608277-92608296 MS.gene80201:CDS 55.0%
!! GGTGTTGTTCTTCGGCCTGT+TGG + chr3.4:92607891-92607910 MS.gene80201:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 92607747 92608574 92607747 ID=MS.gene80201
chr3.4 mRNA 92607747 92608574 92607747 ID=MS.gene80201.t1;Parent=MS.gene80201
chr3.4 exon 92607747 92608574 92607747 ID=MS.gene80201.t1.exon1;Parent=MS.gene80201.t1
chr3.4 CDS 92607747 92608574 92607747 ID=cds.MS.gene80201.t1;Parent=MS.gene80201.t1
Gene Sequence

>MS.gene80201

ATGACGGATTTAACGAAGGATTTACAGAAAGGAGATTCGATTCAAATCAGAGAGGTATGGAACGATAATCTGGAAGAGGAATTTGTGTTGATCCGTGAAATTGTTGATAAGTATAACTATGTTGCGATGGATACAGAGTTTCCTGGTGTTGTTCTTCGGCCTGTTGGGAATTTTAAGCATATTAACGATTTTAACTATCAGACTTTGAAGGATAACGTTGATATGTTGAAGCTGATTCAGTTAGGTTTAACTTTTTCTGATGAGAATGGAAATTTACCTACCTGTGGCACTGATAGTCCTTGCATCTGGCAATTCAATTTTCGCGAGTTTAATGTTAGCGAAGATATTTTCGCTGCTGATTCGATCGAGCTTTTGCGCCAATGCGGAATTGATTTTAAGAAGAACAGCGAGCAAGGAATCGACGTGAACAGATTTGGAGAACTTCTGATGTCATCCGGGATTGTGTTGAATGATAATGTTCATTGGGTTACTTTTCACAGTGGTTATGATTTTGGTTATCTTTTGAAGCTTTTGACGTGCAGGGCTTTGCCGGATACACAAGCTGGATTTTTCGATTTGATCGGGATTTATTTCCCGATTGTGTATGATATCAAACATTTGATGAAGTTCTGTAACAGTCTTCATGGTGGTTTGAACAAGCTTGCGGAATTGCTGGAAGTTGAGAGGGTTGGTGTGTGCCATCAAGCTGGATCGGATAGTTTACTCACAGCTTGTACATTTAGGAAGTTGAGGGAAACATTCTTCAACGGCGAAACAGAGAAATATTCTGGTGTGTTGTATGGATTAGGTGTTGAGAAAACAGACTAA

Protein sequence

>MS.gene80201.t1

MTDLTKDLQKGDSIQIREVWNDNLEEEFVLIREIVDKYNYVAMDTEFPGVVLRPVGNFKHINDFNYQTLKDNVDMLKLIQLGLTFSDENGNLPTCGTDSPCIWQFNFREFNVSEDIFAADSIELLRQCGIDFKKNSEQGIDVNRFGELLMSSGIVLNDNVHWVTFHSGYDFGYLLKLLTCRALPDTQAGFFDLIGIYFPIVYDIKHLMKFCNSLHGGLNKLAELLEVERVGVCHQAGSDSLLTACTFRKLRETFFNGETEKYSGVLYGLGVEKTD