Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050399.t1 | XP_003595135.2 | 93.7 | 127 | 8 | 0 | 48 | 174 | 111 | 237 | 1.50E-57 | 232.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050399.t1 | Q9SZI6 | 42.5 | 120 | 66 | 2 | 52 | 171 | 147 | 263 | 8.0e-15 | 82.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050399.t1 | G7IIE3 | 93.7 | 127 | 8 | 0 | 48 | 174 | 106 | 232 | 1.1e-57 | 232.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050399 | MS.gene050400 | 0.898243 | 6.42E-77 | -1.69E-46 |
| MS.gene050399 | MS.gene058444 | 0.807234 | 5.58E-50 | -1.69E-46 |
| MS.gene050399 | MS.gene065475 | 0.815473 | 9.08E-52 | -1.69E-46 |
| MS.gene050399 | MS.gene08083 | 0.808558 | 2.92E-50 | -1.69E-46 |
| MS.gene050399 | MS.gene29915 | 0.808272 | 3.36E-50 | -1.69E-46 |
| MS.gene050399 | MS.gene36504 | 0.801377 | 9.27E-49 | -1.69E-46 |
| MS.gene050399 | MS.gene38232 | 0.806227 | 9.11E-50 | -1.69E-46 |
| MS.gene050399 | MS.gene46956 | 0.801564 | 8.49E-49 | -1.69E-46 |
| MS.gene050399 | MS.gene63115 | 0.801346 | 9.40E-49 | -1.69E-46 |
| MS.gene050399 | MS.gene64994 | 0.802713 | 4.92E-49 | -1.69E-46 |
| MS.gene050399 | MS.gene73235 | 0.801556 | 8.52E-49 | -1.69E-46 |
| MS.gene050399 | MS.gene81254 | 0.806073 | 9.82E-50 | -1.69E-46 |
| MS.gene050399 | MS.gene92559 | 0.813397 | 2.61E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTGTTTGATCTTGATTT+TGG | 0.229038 | 2.3:-50540229 | None:intergenic |
| TTAATCATTCTCTTCTTTGA+TGG | 0.270861 | 2.3:-50540653 | None:intergenic |
| ATCAAACAAGATCTTATTCT+TGG | 0.275784 | 2.3:+50540240 | MS.gene050399:CDS |
| TTTCAAACAACAGCACTGTT+AGG | 0.320261 | 2.3:+50540780 | MS.gene050399:CDS |
| AACTTCTTTCCCTTCACATA+TGG | 0.359398 | 2.3:-50541637 | None:intergenic |
| ACCATTTGCAGCTGCTGCAA+GGG | 0.392222 | 2.3:-50541590 | None:intergenic |
| GCCCTTGCAGCAGCTGCAAA+TGG | 0.406328 | 2.3:+50541589 | MS.gene050399:CDS |
| CTCTGGAGAAGTGGACCAAC+TGG | 0.407869 | 2.3:+50540836 | MS.gene050399:CDS |
| CACCATTTGCAGCTGCTGCA+AGG | 0.426560 | 2.3:-50541591 | None:intergenic |
| CAAAGAAGAGAATGATTAAG+AGG | 0.444414 | 2.3:+50540657 | MS.gene050399:CDS |
| TCAAACAAGATCTTATTCTT+GGG | 0.457205 | 2.3:+50540241 | MS.gene050399:CDS |
| TTCTCAGTTCCTACTAGATC+AGG | 0.466474 | 2.3:-50540740 | None:intergenic |
| GAGAATGATTAAGAGGATGA+TGG | 0.470043 | 2.3:+50540664 | MS.gene050399:CDS |
| TTCAAACAACAGCACTGTTA+GGG | 0.471012 | 2.3:+50540781 | MS.gene050399:CDS |
| TAGAGAAGAAGATGAAGAAG+TGG | 0.474019 | 2.3:-50540186 | None:intergenic |
| CAAGTATCACCTGATCTAGT+AGG | 0.482391 | 2.3:+50540731 | MS.gene050399:CDS |
| GAGGATGATGGAAGATCAAA+GGG | 0.487515 | 2.3:+50540676 | MS.gene050399:CDS |
| TTGGTCCACTTCTCCAGAGA+GGG | 0.496789 | 2.3:-50540832 | None:intergenic |
| CTACACCATCACTTAGTATG+TGG | 0.505698 | 2.3:-50540271 | None:intergenic |
| ACACAACTAAGACCCCTCTC+TGG | 0.508622 | 2.3:+50540819 | MS.gene050399:CDS |
| AATGTGCACCACAATTCAAA+AGG | 0.514128 | 2.3:+50541691 | MS.gene050399:CDS |
| GGAAAGAAGTTGCAAATCAA+AGG | 0.523496 | 2.3:+50541649 | MS.gene050399:CDS |
| GTTGATTAGTATTGAAGAGA+TGG | 0.528693 | 2.3:-50540160 | None:intergenic |
| AAGTTGCAAATCAAAGGAAA+AGG | 0.532008 | 2.3:+50541655 | MS.gene050399:CDS |
| TGCACCACAATTCAAAAGGA+AGG | 0.532928 | 2.3:+50541695 | MS.gene050399:CDS |
| AGAGGATGATGGAAGATCAA+AGG | 0.536944 | 2.3:+50540675 | MS.gene050399:CDS |
| AGGATAGAACTTCAATGAAG+TGG | 0.543169 | 2.3:+50540627 | MS.gene050399:intron |
| AAGACCCCTCTCTGGAGAAG+TGG | 0.553472 | 2.3:+50540827 | MS.gene050399:CDS |
| GGCAGAGAAACCATATGTGA+AGG | 0.565007 | 2.3:+50541627 | MS.gene050399:CDS |
| CATACTAAGTGATGGTGTAG+AGG | 0.591541 | 2.3:+50540274 | MS.gene050399:CDS |
| AAGTGGACCAACTGGTCCCA+AGG | 0.591915 | 2.3:+50540844 | MS.gene050399:CDS |
| AGAGAGGGGTCTTAGTTGTG+TGG | 0.598153 | 2.3:-50540817 | None:intergenic |
| ATATGTACCTTGGGACCAGT+TGG | 0.609719 | 2.3:-50540851 | None:intergenic |
| TGGTCCACTTCTCCAGAGAG+GGG | 0.626088 | 2.3:-50540831 | None:intergenic |
| GCAGAGAAACCATATGTGAA+GGG | 0.631828 | 2.3:+50541628 | MS.gene050399:CDS |
| TGCAAATGGTGAGACTCTTG+TGG | 0.634220 | 2.3:+50541603 | MS.gene050399:CDS |
| GCACCACAATTCAAAAGGAA+GGG | 0.637198 | 2.3:+50541696 | MS.gene050399:CDS |
| TCAAACCACATACTAAGTGA+TGG | 0.647670 | 2.3:+50540266 | MS.gene050399:CDS |
| CAGTCACTTTGCAATGCCTG+CGG | 0.658493 | 2.3:+50541541 | MS.gene050399:intron |
| GTTGGTCCACTTCTCCAGAG+AGG | 0.682429 | 2.3:-50540833 | None:intergenic |
| AAATGGTGAGACTCTTGTGG+TGG | 0.684853 | 2.3:+50541606 | MS.gene050399:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGATAATAATGAAATTTTA+GGG | + | chr2.3:50540601-50540620 | MS.gene050399:intron | 10.0% |
| !! | CAATAGTAATAAAAAAGAAT+TGG | - | chr2.3:50541019-50541038 | None:intergenic | 15.0% |
| !! | TAAACAAAGGTTATTAATTA+TGG | + | chr2.3:50541247-50541266 | MS.gene050399:intron | 15.0% |
| !!! | GAAGATAATAATGAAATTTT+AGG | + | chr2.3:50540600-50540619 | MS.gene050399:intron | 15.0% |
| !!! | TTACTATTGAGAAAATTTTA+TGG | + | chr2.3:50541030-50541049 | MS.gene050399:intron | 15.0% |
| !! | ACTTTAATGATTGTAAACAA+AGG | + | chr2.3:50541234-50541253 | MS.gene050399:intron | 20.0% |
| !! | ATATCAGATAACAATTCAAA+TGG | + | chr2.3:50541057-50541076 | MS.gene050399:intron | 20.0% |
| !! | TACAAAATTCTTAACCTAAA+AGG | - | chr2.3:50540520-50540539 | None:intergenic | 20.0% |
| !! | TGTAATTATTTGCAACATAA+TGG | - | chr2.3:50541284-50541303 | None:intergenic | 20.0% |
| !!! | GGTTAATTAATTTGTATAAC+AGG | + | chr2.3:50540988-50541007 | MS.gene050399:intron | 20.0% |
| ! | ATCAAACAAGATCTTATTCT+TGG | + | chr2.3:50540240-50540259 | MS.gene050399:CDS | 25.0% |
| ! | TCAAACAAGATCTTATTCTT+GGG | + | chr2.3:50540241-50540260 | MS.gene050399:CDS | 25.0% |
| ! | TTAATCATTCTCTTCTTTGA+TGG | - | chr2.3:50540656-50540675 | None:intergenic | 25.0% |
| !! | TTCATGATTAAGCTCTTTTA+TGG | + | chr2.3:50541397-50541416 | MS.gene050399:intron | 25.0% |
| !!! | ATAATGAAATTTTAGGGATG+AGG | + | chr2.3:50540607-50540626 | MS.gene050399:intron | 25.0% |
| !!! | ATCTTGTTTGATCTTGATTT+TGG | - | chr2.3:50540232-50540251 | None:intergenic | 25.0% |
| !!! | TGTTTTAAAGATGAAGTTTG+AGG | + | chr2.3:50540967-50540986 | MS.gene050399:intron | 25.0% |
| AAGTTGCAAATCAAAGGAAA+AGG | + | chr2.3:50541655-50541674 | MS.gene050399:CDS | 30.0% | |
| AGGAAAAGGATGAAAAAGAT+TGG | + | chr2.3:50540571-50540590 | MS.gene050399:intron | 30.0% | |
| ATATTAGATGTCAGTCTATG+AGG | + | chr2.3:50541090-50541109 | MS.gene050399:intron | 30.0% | |
| CAAAGAAGAGAATGATTAAG+AGG | + | chr2.3:50540657-50540676 | MS.gene050399:CDS | 30.0% | |
| GAAAACTAAAGCCTCTATTA+TGG | - | chr2.3:50541137-50541156 | None:intergenic | 30.0% | |
| GAACAAATCTTATACAAGCA+AGG | - | chr2.3:50541380-50541399 | None:intergenic | 30.0% | |
| TTGTTTCAGAACCATAATAG+AGG | + | chr2.3:50541123-50541142 | MS.gene050399:intron | 30.0% | |
| ! | GTTGATTAGTATTGAAGAGA+TGG | - | chr2.3:50540163-50540182 | None:intergenic | 30.0% |
| AACTTCTTTCCCTTCACATA+TGG | - | chr2.3:50541640-50541659 | None:intergenic | 35.0% | |
| AATGTGCACCACAATTCAAA+AGG | + | chr2.3:50541691-50541710 | MS.gene050399:CDS | 35.0% | |
| AGGATAGAACTTCAATGAAG+TGG | + | chr2.3:50540627-50540646 | MS.gene050399:intron | 35.0% | |
| GAGAATGATTAAGAGGATGA+TGG | + | chr2.3:50540664-50540683 | MS.gene050399:CDS | 35.0% | |
| GGAAAGAAGTTGCAAATCAA+AGG | + | chr2.3:50541649-50541668 | MS.gene050399:CDS | 35.0% | |
| GTTAATGCGAAGTTATATCG+AGG | - | chr2.3:50541435-50541454 | None:intergenic | 35.0% | |
| TAGAGAAGAAGATGAAGAAG+TGG | - | chr2.3:50540189-50540208 | None:intergenic | 35.0% | |
| TCAAACCACATACTAAGTGA+TGG | + | chr2.3:50540266-50540285 | MS.gene050399:CDS | 35.0% | |
| TGTACCTTCTAGTGAATCAT+GGG | + | chr2.3:50540536-50540555 | MS.gene050399:intron | 35.0% | |
| TTCAAACAACAGCACTGTTA+GGG | + | chr2.3:50540781-50540800 | MS.gene050399:CDS | 35.0% | |
| TTGTACCTTCTAGTGAATCA+TGG | + | chr2.3:50540535-50540554 | MS.gene050399:intron | 35.0% | |
| TTTCAAACAACAGCACTGTT+AGG | + | chr2.3:50540780-50540799 | MS.gene050399:CDS | 35.0% | |
| ! | CATGTGATTGTCATCCTTTT+AGG | + | chr2.3:50540503-50540522 | MS.gene050399:intron | 35.0% |
| ! | GATTAAGCTCTTTTATGGCT+AGG | + | chr2.3:50541402-50541421 | MS.gene050399:intron | 35.0% |
| ! | TTACCCTTCCTTTTGAATTG+TGG | - | chr2.3:50541702-50541721 | None:intergenic | 35.0% |
| !! | TTATGGTTCTGAAACAATGC+AGG | - | chr2.3:50541120-50541139 | None:intergenic | 35.0% |
| ATCATGGGATCATCAAGAAG+AGG | + | chr2.3:50540551-50540570 | MS.gene050399:intron | 40.0% | |
| CAAGTATCACCTGATCTAGT+AGG | + | chr2.3:50540731-50540750 | MS.gene050399:CDS | 40.0% | |
| CTACACCATCACTTAGTATG+TGG | - | chr2.3:50540274-50540293 | None:intergenic | 40.0% | |
| GCACCACAATTCAAAAGGAA+GGG | + | chr2.3:50541696-50541715 | MS.gene050399:CDS | 40.0% | |
| GCAGAGAAACCATATGTGAA+GGG | + | chr2.3:50541628-50541647 | MS.gene050399:CDS | 40.0% | |
| GGATCATCAAGAAGAGGAAA+AGG | + | chr2.3:50540557-50540576 | MS.gene050399:intron | 40.0% | |
| TGATCCCATGATTCACTAGA+AGG | - | chr2.3:50540543-50540562 | None:intergenic | 40.0% | |
| TGCACCACAATTCAAAAGGA+AGG | + | chr2.3:50541695-50541714 | MS.gene050399:CDS | 40.0% | |
| TTCTCAGTTCCTACTAGATC+AGG | - | chr2.3:50540743-50540762 | None:intergenic | 40.0% | |
| ! | CATACTAAGTGATGGTGTAG+AGG | + | chr2.3:50540274-50540293 | MS.gene050399:CDS | 40.0% |
| !! | AGAGGATGATGGAAGATCAA+AGG | + | chr2.3:50540675-50540694 | MS.gene050399:CDS | 40.0% |
| !! | GAGGATGATGGAAGATCAAA+GGG | + | chr2.3:50540676-50540695 | MS.gene050399:CDS | 40.0% |
| AAATGGTGAGACTCTTGTGG+TGG | + | chr2.3:50541606-50541625 | MS.gene050399:CDS | 45.0% | |
| ATATGTACCTTGGGACCAGT+TGG | - | chr2.3:50540854-50540873 | None:intergenic | 45.0% | |
| CCCAAGGTACATATTGCTCA+CGG | + | chr2.3:50540860-50540879 | MS.gene050399:intron | 45.0% | |
| CCGTGAGCAATATGTACCTT+GGG | - | chr2.3:50540863-50540882 | None:intergenic | 45.0% | |
| GGCAGAGAAACCATATGTGA+AGG | + | chr2.3:50541627-50541646 | MS.gene050399:CDS | 45.0% | |
| TCCGTGAGCAATATGTACCT+TGG | - | chr2.3:50540864-50540883 | None:intergenic | 45.0% | |
| TGCAAATGGTGAGACTCTTG+TGG | + | chr2.3:50541603-50541622 | MS.gene050399:CDS | 45.0% | |
| ! | TTTTCTTTGTCGAATGCCGC+AGG | - | chr2.3:50541560-50541579 | None:intergenic | 45.0% |
| !!! | AGATATATATAAAATTTTAA+AGG | - | chr2.3:50540348-50540367 | None:intergenic | 5.0% |
| !!! | ATATATATAAAATTTTAAAG+GGG | - | chr2.3:50540346-50540365 | None:intergenic | 5.0% |
| !!! | GAAAAAAATATTATTTTAAA+AGG | - | chr2.3:50540892-50540911 | None:intergenic | 5.0% |
| !!! | GATATATATAAAATTTTAAA+GGG | - | chr2.3:50540347-50540366 | None:intergenic | 5.0% |
| ACACAACTAAGACCCCTCTC+TGG | + | chr2.3:50540819-50540838 | MS.gene050399:CDS | 50.0% | |
| ACCATTTGCAGCTGCTGCAA+GGG | - | chr2.3:50541593-50541612 | None:intergenic | 50.0% | |
| AGAGAGGGGTCTTAGTTGTG+TGG | - | chr2.3:50540820-50540839 | None:intergenic | 50.0% | |
| TTGGTCCACTTCTCCAGAGA+GGG | - | chr2.3:50540835-50540854 | None:intergenic | 50.0% | |
| ! | CAGTCACTTTGCAATGCCTG+CGG | + | chr2.3:50541541-50541560 | MS.gene050399:intron | 50.0% |
| AAGTGGACCAACTGGTCCCA+AGG | + | chr2.3:50540844-50540863 | MS.gene050399:CDS | 55.0% | |
| CACCATTTGCAGCTGCTGCA+AGG | - | chr2.3:50541594-50541613 | None:intergenic | 55.0% | |
| GTTGGTCCACTTCTCCAGAG+AGG | - | chr2.3:50540836-50540855 | None:intergenic | 55.0% | |
| TGGTCCACTTCTCCAGAGAG+GGG | - | chr2.3:50540834-50540853 | None:intergenic | 55.0% | |
| ! | AAGACCCCTCTCTGGAGAAG+TGG | + | chr2.3:50540827-50540846 | MS.gene050399:CDS | 55.0% |
| ! | CTCTGGAGAAGTGGACCAAC+TGG | + | chr2.3:50540836-50540855 | MS.gene050399:CDS | 55.0% |
| GCCCTTGCAGCAGCTGCAAA+TGG | + | chr2.3:50541589-50541608 | MS.gene050399:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 50540150 | 50541756 | 50540150 | ID=MS.gene050399 |
| chr2.3 | mRNA | 50540150 | 50541756 | 50540150 | ID=MS.gene050399.t1;Parent=MS.gene050399 |
| chr2.3 | exon | 50540150 | 50540295 | 50540150 | ID=MS.gene050399.t1.exon1;Parent=MS.gene050399.t1 |
| chr2.3 | CDS | 50540150 | 50540295 | 50540150 | ID=cds.MS.gene050399.t1;Parent=MS.gene050399.t1 |
| chr2.3 | exon | 50540629 | 50540865 | 50540629 | ID=MS.gene050399.t1.exon2;Parent=MS.gene050399.t1 |
| chr2.3 | CDS | 50540629 | 50540865 | 50540629 | ID=cds.MS.gene050399.t1;Parent=MS.gene050399.t1 |
| chr2.3 | exon | 50541544 | 50541756 | 50541544 | ID=MS.gene050399.t1.exon3;Parent=MS.gene050399.t1 |
| chr2.3 | CDS | 50541544 | 50541756 | 50541544 | ID=cds.MS.gene050399.t1;Parent=MS.gene050399.t1 |
| Gene Sequence |
| Protein sequence |
